Junqi Jin, Qiao He, Shuqin Li, Xuan Zhou, Yongqiao Fang
The absence of organic solutions during the preparation of waterborne polyurethane has resulted in a decline in its overall performance. To address this issue, this study introduces nano zirconia as a modifier to enhance the properties of the polyurethane material. A novel composite material combining waterborne polyurethane and zirconium oxide nanoparticles (ZrO 2 NPs) was proposed, and its performance was experimentally analyzed. The particle size of ZrO 2 @CA is as high as 80 nm, which exceeds the 23 nm of ZrO 2 @MA and the 21 nm of ZrO 2 @AA. Transmission electron microscopy reveals that the emulsion droplets in the composite lotion exhibit excellent spherical uniformity and dispersion. Moreover, there is not a significant difference in size between composite lotions with different zirconia contents. Remarkably, even with a zirconia content of only 0.5 wt%, the specimen exhibits high mechanical properties, with the tensile strength increasing from 11.9 MPa to 14.3 MPa and the elongation at break increasing from 560% to 712%. Additionally, a higher doping amount of ZrO 2 leads to an increased water absorption rate, with the actual water absorption rate rising from 7% to 14.4%. In practical engineering projects A and B, the initial water output of four holes in engineering A is maintained at 48–580 m 3 /h. However, as time progresses, the water output gradually decreases, approaching 0 m 3 /h. In project B, the composite material coating exhibits a tensile strength greater than 15 MPa, reaching 18.26 MPa, which significantly surpasses the performance of the comparison material. Overall, the composite materials demonstrate excellent mechanical and water protection properties, proving to be crucial for practical water inrush protection applications.
{"title":"Properties of waterborne polyurethane chemically modified composite materials and their application in gushing water protection","authors":"Junqi Jin, Qiao He, Shuqin Li, Xuan Zhou, Yongqiao Fang","doi":"10.1166/mex.2023.2531","DOIUrl":"https://doi.org/10.1166/mex.2023.2531","url":null,"abstract":"The absence of organic solutions during the preparation of waterborne polyurethane has resulted in a decline in its overall performance. To address this issue, this study introduces nano zirconia as a modifier to enhance the properties of the polyurethane material. A novel composite material combining waterborne polyurethane and zirconium oxide nanoparticles (ZrO 2 NPs) was proposed, and its performance was experimentally analyzed. The particle size of ZrO 2 @CA is as high as 80 nm, which exceeds the 23 nm of ZrO 2 @MA and the 21 nm of ZrO 2 @AA. Transmission electron microscopy reveals that the emulsion droplets in the composite lotion exhibit excellent spherical uniformity and dispersion. Moreover, there is not a significant difference in size between composite lotions with different zirconia contents. Remarkably, even with a zirconia content of only 0.5 wt%, the specimen exhibits high mechanical properties, with the tensile strength increasing from 11.9 MPa to 14.3 MPa and the elongation at break increasing from 560% to 712%. Additionally, a higher doping amount of ZrO 2 leads to an increased water absorption rate, with the actual water absorption rate rising from 7% to 14.4%. In practical engineering projects A and B, the initial water output of four holes in engineering A is maintained at 48–580 m 3 /h. However, as time progresses, the water output gradually decreases, approaching 0 m 3 /h. In project B, the composite material coating exhibits a tensile strength greater than 15 MPa, reaching 18.26 MPa, which significantly surpasses the performance of the comparison material. Overall, the composite materials demonstrate excellent mechanical and water protection properties, proving to be crucial for practical water inrush protection applications.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"62 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135323184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To effectively promote the healing of periodontal bone defect (BD), an innovative approach was adopted, utilizing a combination of chitosan scaffold (CS) and dental pulp stem cells (DPSCs) for periodontal regeneration. The CS was prepared with a pore size of approximately 150 μ m and a porosity of 75%, exhibiting water absorption and swelling rates of 90% and 72%, respectively. Furthermore, the CS exhibited excellent antibacterial properties and effectively inhibited the proliferation activity of Porphyromonas gingivalis , thereby creating a favorable environment for DPSCs to proliferate. In cellular experiments, CS effectively enhanced the activity of DPSCs and demonstrated good osteoconductivity and osteoinduction, effectively enhancing the migration and mineralization ability of DPSCs. In animal studies, the combined application of CS and DPSCs improved the repair of periodontal BD, promoted the regeneration of bone trabecular, and increased bone density at the defect site. Therefore, the combined application of CS and DPSCs holds significant potential for the treatment of periodontal BD.
{"title":"Enhanced activity and differentiation of periodontal ligament stem cells by chitosan scaffold and its application in periodontal bone regeneration","authors":"Guoqiang Zhou, Ye Liu, Ding Wang, Fengqiu Zhang","doi":"10.1166/mex.2023.2509","DOIUrl":"https://doi.org/10.1166/mex.2023.2509","url":null,"abstract":"To effectively promote the healing of periodontal bone defect (BD), an innovative approach was adopted, utilizing a combination of chitosan scaffold (CS) and dental pulp stem cells (DPSCs) for periodontal regeneration. The CS was prepared with a pore size of approximately 150 μ m and a porosity of 75%, exhibiting water absorption and swelling rates of 90% and 72%, respectively. Furthermore, the CS exhibited excellent antibacterial properties and effectively inhibited the proliferation activity of Porphyromonas gingivalis , thereby creating a favorable environment for DPSCs to proliferate. In cellular experiments, CS effectively enhanced the activity of DPSCs and demonstrated good osteoconductivity and osteoinduction, effectively enhancing the migration and mineralization ability of DPSCs. In animal studies, the combined application of CS and DPSCs improved the repair of periodontal BD, promoted the regeneration of bone trabecular, and increased bone density at the defect site. Therefore, the combined application of CS and DPSCs holds significant potential for the treatment of periodontal BD.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"113 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135324742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hydrogel of methylcoumarino-tripeptidospiropyran (MC-TPSP) with a conformational transition at the C-terminus of the peptide was synthesised in this study. This LMWG can be shifted between gel and solution due to the isomerization of spiropyran- and merocyanine-typed MC-TPSP and MC-TPMC, respectively. UV was used to investigate the isomerisation process, and SEM was used to characterise the morphology of the MC-TPMC. The transition temperature was determined to be 75 °C, and the minimum gel concentration was 14.5 mM. On account of its structural biocompatibility and responsive regulatory mechanism, it is anticipated that it will develop into an outstanding bio-functional material.
{"title":"Synthesis and gelation character of photothermal responsive methylcoumarinotripeptidospiropyran","authors":"Guang Tian, Chuanguang Qin","doi":"10.1166/mex.2023.2522","DOIUrl":"https://doi.org/10.1166/mex.2023.2522","url":null,"abstract":"Hydrogel of methylcoumarino-tripeptidospiropyran (MC-TPSP) with a conformational transition at the C-terminus of the peptide was synthesised in this study. This LMWG can be shifted between gel and solution due to the isomerization of spiropyran- and merocyanine-typed MC-TPSP and MC-TPMC, respectively. UV was used to investigate the isomerisation process, and SEM was used to characterise the morphology of the MC-TPMC. The transition temperature was determined to be 75 °C, and the minimum gel concentration was 14.5 mM. On account of its structural biocompatibility and responsive regulatory mechanism, it is anticipated that it will develop into an outstanding bio-functional material.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"113 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135323172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Due to the increased incidence of cardiovascular diseases, the incidence of cardiac ischemia/reperfusion injury (IRI) is also on the rise. Luteolin has a certain protective effect on myocardium. Therefore, this study focuses on whether luteolin can protect IRI in rats with myocardial infarction. A myocardial infarction rat model was prepared and the isolated heart was treated to induce cardiac ischemia-reperfusion injury. British biodegradable packaging company (BIOPAC) biological signal collector detects myocardial indicators; semi-automatic biochemical analyzer detects myocardial infarct size; hematoxylin-eosin (HE) staining observes pathological changes along with analysis of the levels of ERK, JNK, and Caspase-3 and proliferation and apoptosis of myocardial cells. Luteolin can improve the myocardial index level of rats with myocardial infarction and increase survival rate; the rats in luteolin group have the smallest myocardial infarction area, and the myocardial tissue is tightly arranged, the myocardial collagen is significantly reduced, and the myocardial injury index is significantly reduced. Luteolin inhibited the proliferation, migration and apoptosis of cardiomyocytes. Caspase-3, ERK, and JNK expression in the ERK/JNK agonist group was the most obvious. Their levels were highest in the luteolin+ERK/JNK agonist group (vs. other groups, P <0.05), which was higher than ERK/JNK agonist group. Luteolin can inhibit Caspase-3 expression by activating ERK/JNK signaling pathway, thereby reducing myocardial injury.
{"title":"Protective effect of luteolin on cardiac ischemia/reperfusion injury in rats with myocardial infarction through ERK/JNK signaling pathway","authors":"Meng Liu, Lei Lu, Ling Yuan, Xiaohu Chen","doi":"10.1166/mex.2023.2524","DOIUrl":"https://doi.org/10.1166/mex.2023.2524","url":null,"abstract":"Due to the increased incidence of cardiovascular diseases, the incidence of cardiac ischemia/reperfusion injury (IRI) is also on the rise. Luteolin has a certain protective effect on myocardium. Therefore, this study focuses on whether luteolin can protect IRI in rats with myocardial infarction. A myocardial infarction rat model was prepared and the isolated heart was treated to induce cardiac ischemia-reperfusion injury. British biodegradable packaging company (BIOPAC) biological signal collector detects myocardial indicators; semi-automatic biochemical analyzer detects myocardial infarct size; hematoxylin-eosin (HE) staining observes pathological changes along with analysis of the levels of ERK, JNK, and Caspase-3 and proliferation and apoptosis of myocardial cells. Luteolin can improve the myocardial index level of rats with myocardial infarction and increase survival rate; the rats in luteolin group have the smallest myocardial infarction area, and the myocardial tissue is tightly arranged, the myocardial collagen is significantly reduced, and the myocardial injury index is significantly reduced. Luteolin inhibited the proliferation, migration and apoptosis of cardiomyocytes. Caspase-3, ERK, and JNK expression in the ERK/JNK agonist group was the most obvious. Their levels were highest in the luteolin+ERK/JNK agonist group (vs. other groups, P <0.05), which was higher than ERK/JNK agonist group. Luteolin can inhibit Caspase-3 expression by activating ERK/JNK signaling pathway, thereby reducing myocardial injury.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135324547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mixing limestone powder (LP) in the self-leveling mortar (SLM) can not only solve the problems of LP waste randomly piled up and secondary utilization of resources, but also reduce the raw material cost of SLM and have excellent mechanical properties. The effect of replacing fly ash (FA) with LP and replacing cement with LP after completely replacing FA on fluidity and strength of SLM are studied. The microstructure of SLM is measured by mercury intrusion porosimetry and scanning electron microscope. The results show that the initial fluidity and the 20-min fluidity of SLM decrease gradually with the increase of LP content. The strength of SLM increases and then decreases with the increase of LP replacing FA, and the strength is the highest when the addition of LP is 40%. When LP replaces cement after completely replacing FA, the strength of SLM decreases with the increase of displacement. Excessive LP can greatly damage the mechanical properties of SLM. The appropriate content of LP can improve the microstructure of SLM and promote the formation of hydration products, which is helpful to reduce the porosity and thus improves the structure density. This may be due to the chemical reaction and the microfiller effect of LP.
{"title":"Effect of limestone powder on properties of self-leveling mortar","authors":"Hao Xu, Fei-Jin Chen, Hong-Yu Tao, Yi-Feng He, Wen-Qiang Zhai, Jian-Ping Zhang, Zhi-Hai He","doi":"10.1166/mex.2023.2523","DOIUrl":"https://doi.org/10.1166/mex.2023.2523","url":null,"abstract":"Mixing limestone powder (LP) in the self-leveling mortar (SLM) can not only solve the problems of LP waste randomly piled up and secondary utilization of resources, but also reduce the raw material cost of SLM and have excellent mechanical properties. The effect of replacing fly ash (FA) with LP and replacing cement with LP after completely replacing FA on fluidity and strength of SLM are studied. The microstructure of SLM is measured by mercury intrusion porosimetry and scanning electron microscope. The results show that the initial fluidity and the 20-min fluidity of SLM decrease gradually with the increase of LP content. The strength of SLM increases and then decreases with the increase of LP replacing FA, and the strength is the highest when the addition of LP is 40%. When LP replaces cement after completely replacing FA, the strength of SLM decreases with the increase of displacement. Excessive LP can greatly damage the mechanical properties of SLM. The appropriate content of LP can improve the microstructure of SLM and promote the formation of hydration products, which is helpful to reduce the porosity and thus improves the structure density. This may be due to the chemical reaction and the microfiller effect of LP.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"114 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135324308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chunyan Hu, Weiqiang Wang, Haoxi Yuan, Juan Li, Zuliang Liu, Ying Cao
This study aimed to compare the clinical efficacy of laparoscopic sacrocolpopexy (LSC) and laparoscopic pectopexy (LP) for the treatment of pelvic organ prolapse (POP) and to evaluate the antibacterial effect of nanosilver-coated urethral catheters. The clinical data of 112 patients with POP, including 56 patients who underwent LP and 56 patients who underwent LSC, from March 1, 2019 to March 31, 2021 were retrospectively evaluated. All patients underwent routine catheterization with nanosilver-coated urethral catheters after surgery. The baseline data, intraoperative and postoperative efficacy, serum inflammatory indexes and postoperative complications were compared. The surgery was successfully completed in both groups, and a total of 10 patients in both group underwent intraoperative repair of the anterior and posterior vaginal walls. The surgical duration was significantly shorter and the intraoperative blood loss was significantly lower in the LP group than in the LSC group ( P <0.05 for both). In both groups, the POP quantification stage, which was ≥I prior to surgery, was ≤I at six months after surgery. The PFIQ-7, PFDI-20 and PISQ-12 scores were significantly improved in both groups ( P <0.05 for all), and the improvements were significantly better in the LP group than in the LSC group ( P <0.01). During the 6-month follow-up period, no patient developed urinary tract infection. The levels of postoperative serum inflammatory factors WBC, ESR and CRP were increased in the LSC group and higher than those in the LP group, highlighting the significant anti-infection effect of the nanosilver-coated catheter. Mesh exposure occurred in one patient in the LSC group. In the LSC group, postoperative symptoms including abnormal defecation, lower abdominal pain/soreness, new stress urinary incontinence, and dyspareunia. In the LP group, abnormal defecation and lower abdominal pain were present in one patient each. The rate of postoperative complications was higher in the LSC group than in the LP group. While both LSC and LP could restore pelvic floor anatomic structure and organ function in patients with POP, shorter surgical duration, less blood loss, and better therapeutic effect were observed in patients undergoing LP. Nanosilver-coated antibacterial catheters exhibited good antibacterial effect in patients with routine catheterization after surgery. This study provides a new treatment plan for the treatment of pelvic organ prolapse, and also provides a new nursing method for postoperative urinary tract infection.
{"title":"Efficacy of laparoscopic pectopexy versus laparoscopic sacrocolpopexy and antibacterial performance of nanosilver-coated urethral catheter for postoperative catheterization in patients with pelvic organ prolapse","authors":"Chunyan Hu, Weiqiang Wang, Haoxi Yuan, Juan Li, Zuliang Liu, Ying Cao","doi":"10.1166/mex.2023.2515","DOIUrl":"https://doi.org/10.1166/mex.2023.2515","url":null,"abstract":"This study aimed to compare the clinical efficacy of laparoscopic sacrocolpopexy (LSC) and laparoscopic pectopexy (LP) for the treatment of pelvic organ prolapse (POP) and to evaluate the antibacterial effect of nanosilver-coated urethral catheters. The clinical data of 112 patients with POP, including 56 patients who underwent LP and 56 patients who underwent LSC, from March 1, 2019 to March 31, 2021 were retrospectively evaluated. All patients underwent routine catheterization with nanosilver-coated urethral catheters after surgery. The baseline data, intraoperative and postoperative efficacy, serum inflammatory indexes and postoperative complications were compared. The surgery was successfully completed in both groups, and a total of 10 patients in both group underwent intraoperative repair of the anterior and posterior vaginal walls. The surgical duration was significantly shorter and the intraoperative blood loss was significantly lower in the LP group than in the LSC group ( P <0.05 for both). In both groups, the POP quantification stage, which was ≥I prior to surgery, was ≤I at six months after surgery. The PFIQ-7, PFDI-20 and PISQ-12 scores were significantly improved in both groups ( P <0.05 for all), and the improvements were significantly better in the LP group than in the LSC group ( P <0.01). During the 6-month follow-up period, no patient developed urinary tract infection. The levels of postoperative serum inflammatory factors WBC, ESR and CRP were increased in the LSC group and higher than those in the LP group, highlighting the significant anti-infection effect of the nanosilver-coated catheter. Mesh exposure occurred in one patient in the LSC group. In the LSC group, postoperative symptoms including abnormal defecation, lower abdominal pain/soreness, new stress urinary incontinence, and dyspareunia. In the LP group, abnormal defecation and lower abdominal pain were present in one patient each. The rate of postoperative complications was higher in the LSC group than in the LP group. While both LSC and LP could restore pelvic floor anatomic structure and organ function in patients with POP, shorter surgical duration, less blood loss, and better therapeutic effect were observed in patients undergoing LP. Nanosilver-coated antibacterial catheters exhibited good antibacterial effect in patients with routine catheterization after surgery. This study provides a new treatment plan for the treatment of pelvic organ prolapse, and also provides a new nursing method for postoperative urinary tract infection.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135324554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present study evaluated an improved Enzyme linked immunosorbent assay (ELISA) method based on gold nanoparticles by investigating the correlations of measured serum occludin and zonula occludin-1 (ZO-1) levels with cognitive impairment in elderly patients with acute cerebral infarction (ACI). The present study comprised a total of 98 patients with ACI that were divided into patients with normal cognitive function (normal group) and patients with cognitive impairment (PSCI group) according to mini-mental state examination (MMSE) scores. Serum levels of triglycerides, total cholesterol, and low-density lipoprotein were measured using an automatic biochemical analyzer. Serum occludin and ZO-1 levels in both groups were measured using a modified ELISA assay with nanogold particles. Neurological function in elderly patients with ACI was assessed using the National Institutes of Health Stroke Scale (NIHSS) score. Pearson’s correlation coefficient was calculated for the correlations between serum occludin levels, serum ZO-1 levels, and cognitive impairment in elderly patients with ACI. Receiver operating characteristics (ROC) curves were used to analyze the diagnostic value of serum occludin and ZO-1 levels in detecting cognitive impairment in elderly patients with ACI. Logistic regression analysis was used to identify factors associated with cognitive impairment in elderly patients with ACI. In the PSCI group, a significantly higher proportion of patients had hypertension, coronary heart disease, and diabetes compared to the normal group. Serum occludin and ZO-1 levels were significantly lower in the PSCI group compared to the normal group ( P <0.05). Serum occludin and ZO-1 levels in elderly patients with ACI were negatively associated with NIHSS score ( P <0.05). Receiver operating characteristic analysis demonstrated that the area under the curve (AUC) of serum occludin and ZO-1 in detecting cognitive impairment in elderly patients with ACI was 0.926 and 0.824 respectively, with a combined AUC of 0.945 indicating a synergistic effect in detecting cognitive impairment. Logistic regression analysis showed that hypertension, diabetes, coronary heart disease, and lower serum occludin, and ZO-1 levels were all independent risk factors for cognitive impairment in elderly patients with ACI. Lower serum levels of occludin and ZO-1 level had diagnostic value in detecting cognitive impairment in elderly patients with ACI and may have utility as an auxiliary diagnostic index for cognitive dysfunction in patients with ACI.
{"title":"Development of an improved ELISA method based on gold nanoparticles to evaluate the correlation of serum occludin and zonula occludin-1 (ZO-1) levels with cognitive impairment in elderly patients with acute cerebral infarction","authors":"Li Gao, Jianjun Zhang, Jianhui Wei, Jiangang Li","doi":"10.1166/mex.2023.2512","DOIUrl":"https://doi.org/10.1166/mex.2023.2512","url":null,"abstract":"The present study evaluated an improved Enzyme linked immunosorbent assay (ELISA) method based on gold nanoparticles by investigating the correlations of measured serum occludin and zonula occludin-1 (ZO-1) levels with cognitive impairment in elderly patients with acute cerebral infarction (ACI). The present study comprised a total of 98 patients with ACI that were divided into patients with normal cognitive function (normal group) and patients with cognitive impairment (PSCI group) according to mini-mental state examination (MMSE) scores. Serum levels of triglycerides, total cholesterol, and low-density lipoprotein were measured using an automatic biochemical analyzer. Serum occludin and ZO-1 levels in both groups were measured using a modified ELISA assay with nanogold particles. Neurological function in elderly patients with ACI was assessed using the National Institutes of Health Stroke Scale (NIHSS) score. Pearson’s correlation coefficient was calculated for the correlations between serum occludin levels, serum ZO-1 levels, and cognitive impairment in elderly patients with ACI. Receiver operating characteristics (ROC) curves were used to analyze the diagnostic value of serum occludin and ZO-1 levels in detecting cognitive impairment in elderly patients with ACI. Logistic regression analysis was used to identify factors associated with cognitive impairment in elderly patients with ACI. In the PSCI group, a significantly higher proportion of patients had hypertension, coronary heart disease, and diabetes compared to the normal group. Serum occludin and ZO-1 levels were significantly lower in the PSCI group compared to the normal group ( P <0.05). Serum occludin and ZO-1 levels in elderly patients with ACI were negatively associated with NIHSS score ( P <0.05). Receiver operating characteristic analysis demonstrated that the area under the curve (AUC) of serum occludin and ZO-1 in detecting cognitive impairment in elderly patients with ACI was 0.926 and 0.824 respectively, with a combined AUC of 0.945 indicating a synergistic effect in detecting cognitive impairment. Logistic regression analysis showed that hypertension, diabetes, coronary heart disease, and lower serum occludin, and ZO-1 levels were all independent risk factors for cognitive impairment in elderly patients with ACI. Lower serum levels of occludin and ZO-1 level had diagnostic value in detecting cognitive impairment in elderly patients with ACI and may have utility as an auxiliary diagnostic index for cognitive dysfunction in patients with ACI.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"62 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135323176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this paper, experimental studies of hybrid fibre reinforced polymer (HyFRP) laminates consisting of carbon ply fibre reinforced polymer (CFRP) and glass ply fibre reinforced polymer (GFRP) are reported on in conjunction with studies of concrete beams with high strength (HSC) under the category of with and without HyFRP laminates. The aim of this experimental work is to examine how alternative sequences of CFRP and GFRP ply of hybrid FRPs effect the enhancement of the high strength concrete beams. For the purpose of this study, there are a total of seventeen HSC beams cast. One of the beams was cast without HyFRP to act as a guiding beam, and the residual sixteen beams were cast with HyFRP using reinforced concrete and various combinations of carbon ply fibre reinforced polymer (CFRP) and glass ply fibre reinforced polymer (GFRP). The sequences in which hybrid FRP layers are attached serve as the primary variables in this test. Concrete with reinforcements after laminating beams with two, three, and even four layers of HyFRP, a load was applied in the form of static to the beams until they failed. The findings of the tests lead one to the conclusion that the repercussions of hybrid FRPs on the ductility of HSC beams are strengthened differently depending the order of the FRP layers.
{"title":"Behaviour of hybrid fibre reinforced polymer (HyFRP) laminates on high strength concrete (HSC) beams under flexural loading","authors":"P. Somiyadevi, V. Ramasamy","doi":"10.1166/mex.2023.2516","DOIUrl":"https://doi.org/10.1166/mex.2023.2516","url":null,"abstract":"In this paper, experimental studies of hybrid fibre reinforced polymer (HyFRP) laminates consisting of carbon ply fibre reinforced polymer (CFRP) and glass ply fibre reinforced polymer (GFRP) are reported on in conjunction with studies of concrete beams with high strength (HSC) under the category of with and without HyFRP laminates. The aim of this experimental work is to examine how alternative sequences of CFRP and GFRP ply of hybrid FRPs effect the enhancement of the high strength concrete beams. For the purpose of this study, there are a total of seventeen HSC beams cast. One of the beams was cast without HyFRP to act as a guiding beam, and the residual sixteen beams were cast with HyFRP using reinforced concrete and various combinations of carbon ply fibre reinforced polymer (CFRP) and glass ply fibre reinforced polymer (GFRP). The sequences in which hybrid FRP layers are attached serve as the primary variables in this test. Concrete with reinforcements after laminating beams with two, three, and even four layers of HyFRP, a load was applied in the form of static to the beams until they failed. The findings of the tests lead one to the conclusion that the repercussions of hybrid FRPs on the ductility of HSC beams are strengthened differently depending the order of the FRP layers.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135324741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Research reports have found that sinomenine has a potentially inhibitory effect on lung cancer. At the same time, sinomenine and P53 gene show a close relationship with ferroptosis. In order to further discover the mechanism of action, this research intends to evaluate whether sinomenine can drive P53 gene through DKN2A to promote the growth of iron in lung cancer. Lung cancer cells were transfected with DKN2A overexpression plasmid followed by analysis of cell proliferation, expression of DKN2A and P53. Protein expression and cell viability were observed after P53 was inhibited by the chemical inhibitor PFT- α . To further verify histological expression, epithelial injury and apoptosis expression were detected by hematoxylin and eosin stain (H&E) and immunofluorescence. Sinomenine affects activities of lung cancer cells. After sinomenine treatment, cell shape became round, with increased cell shrinkage and death. Overexpression of DKN2A inhibited sinomenine-induced ferroptosis. P53 negatively modulated DKN2A. STAT3 was upregulated with induction of ferroptosis during lung injury. In conclusion, Sinomenine promotes ferroptosis of lung cancer cells by regulating DKN2A and P53, thereby affecting the proliferation of lung cancer cell proliferation.
{"title":"Sinomenine promotes ferroptosis in lung cancer cells by driving P53 gene","authors":"Zhengkai Xiang, Xiang Wang, Bingfeng Li, Xiaochuan Dong","doi":"10.1166/mex.2023.2514","DOIUrl":"https://doi.org/10.1166/mex.2023.2514","url":null,"abstract":"Research reports have found that sinomenine has a potentially inhibitory effect on lung cancer. At the same time, sinomenine and P53 gene show a close relationship with ferroptosis. In order to further discover the mechanism of action, this research intends to evaluate whether sinomenine can drive P53 gene through DKN2A to promote the growth of iron in lung cancer. Lung cancer cells were transfected with DKN2A overexpression plasmid followed by analysis of cell proliferation, expression of DKN2A and P53. Protein expression and cell viability were observed after P53 was inhibited by the chemical inhibitor PFT- α . To further verify histological expression, epithelial injury and apoptosis expression were detected by hematoxylin and eosin stain (H&E) and immunofluorescence. Sinomenine affects activities of lung cancer cells. After sinomenine treatment, cell shape became round, with increased cell shrinkage and death. Overexpression of DKN2A inhibited sinomenine-induced ferroptosis. P53 negatively modulated DKN2A. STAT3 was upregulated with induction of ferroptosis during lung injury. In conclusion, Sinomenine promotes ferroptosis of lung cancer cells by regulating DKN2A and P53, thereby affecting the proliferation of lung cancer cell proliferation.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135324743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hypoxic-ischemic brain damage (HIBD) is a severe complication of neonatal asphyxia that contributes significantly to neonatal mortality, cerebral palsy, and delays in intellectual and motor development. Neuropeptide S (NPS) plays a role in the regulation of various physiological processes. This study aimed to determine the morphological localization of NPS in hypothalamic target neurons during HIBD, providing a basis for further investigation of HIBD. Seven-day-old SD neonatal male rats were assigned to a sham group and a model group to establish the HIBD model. Then, the rats in the model group were further averagely divided into the NPS group and the normal saline group. Immunohistochemical staining of Fos immunoreactivity (Fos-IR) found that NPS administration resulted in a significant increase in the count of Fos-IR neurons in the suprachiasmatic nucleus (122%), paraventricular nucleus (108%), dorsal tuberomammillary nucleus (174% and 386%), ventromedial hypothalamic nucleus (116%), arcuate nucleus (167%), perifornical nucleus (320%), ventral tuberomammillary nucleus (441%), and lateral hypothalamic area (278%) ( P < 0.0001), compared to the normal saline group. During HIBD, NPS can protect the above neurons and activate the above target neurons in the hypothalamus to participate in the sleep and wake cycle, mood, diet, circadian rhythm, temperature and neuroendocrine regulation.
{"title":"Study on the protective mechanism of neuropeptide S in neonates with hypoxic ischemic encephalopathy","authors":"Jun Wang, Qiong Yan","doi":"10.1166/mex.2023.2525","DOIUrl":"https://doi.org/10.1166/mex.2023.2525","url":null,"abstract":"Hypoxic-ischemic brain damage (HIBD) is a severe complication of neonatal asphyxia that contributes significantly to neonatal mortality, cerebral palsy, and delays in intellectual and motor development. Neuropeptide S (NPS) plays a role in the regulation of various physiological processes. This study aimed to determine the morphological localization of NPS in hypothalamic target neurons during HIBD, providing a basis for further investigation of HIBD. Seven-day-old SD neonatal male rats were assigned to a sham group and a model group to establish the HIBD model. Then, the rats in the model group were further averagely divided into the NPS group and the normal saline group. Immunohistochemical staining of Fos immunoreactivity (Fos-IR) found that NPS administration resulted in a significant increase in the count of Fos-IR neurons in the suprachiasmatic nucleus (122%), paraventricular nucleus (108%), dorsal tuberomammillary nucleus (174% and 386%), ventromedial hypothalamic nucleus (116%), arcuate nucleus (167%), perifornical nucleus (320%), ventral tuberomammillary nucleus (441%), and lateral hypothalamic area (278%) ( P < 0.0001), compared to the normal saline group. During HIBD, NPS can protect the above neurons and activate the above target neurons in the hypothalamus to participate in the sleep and wake cycle, mood, diet, circadian rhythm, temperature and neuroendocrine regulation.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135324748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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