This study investigates the efficiency of the combination of nano-silver dressing and vacuum sealing drainage (VSD) with negative pressure treatment on healing diabetic foot ulcers (DFU). A total of 100 patients with DFU admitted to our hospital between January 2022 and February 2023 were selected and randomly divided into a control group and an observation group, each consisting of 50 cases. Both groups received nano-silver dressings after debridement, but the observation group also underwent closed negative pressure drainage. Scores for wound granulation morphology and comfort were assessed before and after treatment. Efficacy rate, healing time, and clinical outcomes were evaluated before and after treatment. Serum levels of procalcitonin (PCT), high-sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6), and cystatin C were measured before treatment and 30 days into treatment. After 10 days of treatment, the observation group showed a higher effective rate (96%) compared to the control group (88%). The observation group had better wound granulation morphology and comfort scores, shorter granulation and healing times, reduced hospitalization duration, and smaller wound areas than the control group. Both groups experienced decreased levels of IL-6, PCT, hs-CRP, and cystatin C after treatment. The observation group had lower levels of these markers compared to the control group. In conclusion, the combination of nano-silver dressing and VSD with negative pressure treatment improves the clinical efficacy of treating DFU. This approach reduces inflammation and promotes wound healing, as evidenced by improved wound scores, faster healing times, and reduced inflammatory marker levels.
{"title":"Effect of combining nano-silver dressing and vacuum sealing drainage with negative pressure treatment on patients with diabetic foot ulcers","authors":"Liang Jin, Chao Niu, Yulong Ni","doi":"10.1166/mex.2023.2566","DOIUrl":"https://doi.org/10.1166/mex.2023.2566","url":null,"abstract":"This study investigates the efficiency of the combination of nano-silver dressing and vacuum sealing drainage (VSD) with negative pressure treatment on healing diabetic foot ulcers (DFU). A total of 100 patients with DFU admitted to our hospital between January 2022 and February 2023\u0000 were selected and randomly divided into a control group and an observation group, each consisting of 50 cases. Both groups received nano-silver dressings after debridement, but the observation group also underwent closed negative pressure drainage. Scores for wound granulation morphology and\u0000 comfort were assessed before and after treatment. Efficacy rate, healing time, and clinical outcomes were evaluated before and after treatment. Serum levels of procalcitonin (PCT), high-sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6), and cystatin C were measured before treatment\u0000 and 30 days into treatment. After 10 days of treatment, the observation group showed a higher effective rate (96%) compared to the control group (88%). The observation group had better wound granulation morphology and comfort scores, shorter granulation and healing times, reduced hospitalization\u0000 duration, and smaller wound areas than the control group. Both groups experienced decreased levels of IL-6, PCT, hs-CRP, and cystatin C after treatment. The observation group had lower levels of these markers compared to the control group. In conclusion, the combination of nano-silver dressing\u0000 and VSD with negative pressure treatment improves the clinical efficacy of treating DFU. This approach reduces inflammation and promotes wound healing, as evidenced by improved wound scores, faster healing times, and reduced inflammatory marker levels.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":" 10","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138617054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In clinical practice, many diseases can lead to changes in serum albumin concentration (HSA) in patients. Accurate detection of HSA concentration is of great significance for disease diagnosis. Based on this, this study designed and synthesized 9-(4-amino-aniline)-acridine (AAA) as a fluorescent probe. By laser induction and capillary gel electrophoresis (CGE), a new rapid and highly specific HSA detection method based on fluorescence-CGE was established. Various experimental control factors were investigated, and the optimal experimental conditions were determined as follows: the running buffer was H3PO4–KH2PO4 (pH = 2.45, 15.0 mmol L−1), the separation voltage was 30 kV, and the experimental temperature was 25 °C. The sample solution injected 10 s with hydrodynamic mode (3.43×103 Pa), HSA could be directly determined by fluorescence-CGE method. The linear range was 0.10–1.0 μg L−1, the detection limit was 0.012 μg L−1, the relative standard deviation (RSD) was less than 0.30%. This method can be used for the determination of real HSA samples. In addition, in the presence of various biological macromolecules, small molecules, ions and ethanol, the accurate detection of HSA by fluorescence-CGE method will not be affected, suggesting that this method has a high specificity for HSA. The rapid and highly specific fluorescence-CGE method of HSA constructed in this study provides a new way to detect HSA, which is of great significance for the diagnosis of clinical diseases.
{"title":"Synthesis of fluorescent 9-(4-aminoaniline)-acridine for highly specific and rapid detection of human serum albumin by fluorescence-capillary gel electrophoresis","authors":"Jing Ye, Tianxiang Hu, Hui Ding, Xiawei Dong","doi":"10.1166/mex.2023.2571","DOIUrl":"https://doi.org/10.1166/mex.2023.2571","url":null,"abstract":"In clinical practice, many diseases can lead to changes in serum albumin concentration (HSA) in patients. Accurate detection of HSA concentration is of great significance for disease diagnosis. Based on this, this study designed and synthesized 9-(4-amino-aniline)-acridine (AAA) as\u0000 a fluorescent probe. By laser induction and capillary gel electrophoresis (CGE), a new rapid and highly specific HSA detection method based on fluorescence-CGE was established. Various experimental control factors were investigated, and the optimal experimental conditions were determined as\u0000 follows: the running buffer was H3PO4–KH2PO4 (pH = 2.45, 15.0 mmol L−1), the separation voltage was 30 kV, and the experimental temperature was 25 °C. The sample solution injected 10 s with hydrodynamic mode (3.43×103\u0000 Pa), HSA could be directly determined by fluorescence-CGE method. The linear range was 0.10–1.0 μg L−1, the detection limit was 0.012 μg L−1, the relative standard deviation (RSD) was less than 0.30%. This method can be used for the\u0000 determination of real HSA samples. In addition, in the presence of various biological macromolecules, small molecules, ions and ethanol, the accurate detection of HSA by fluorescence-CGE method will not be affected, suggesting that this method has a high specificity for HSA. The rapid and\u0000 highly specific fluorescence-CGE method of HSA constructed in this study provides a new way to detect HSA, which is of great significance for the diagnosis of clinical diseases.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":" 3","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138618394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Altwijri, Razan Bakhshwin, E. H. Mirza, Y. A. Algabri, S. Chatpun, Ravish Javed
Annually, more than two million deaths are caused due to the exposure of air pollution which cause damage to the lungs and the respiratory system. Topography, light-textured topsoil, drought, and desert climate make Saudi Arabia vulnerable to sand storms and dust. The study aims to examine the deposition of dust particles in the human airway and its relationship to asthma and investigate the possibility that the dust deposition would be changed with different particle size diameters. An idealized upper respiratory tract 3D model was constructed with computer-aided design software. Later a computational simulation was performed using computational fluid dynamics with 3 different sizes (0.03, 2, and 9 microns) of the dust particles to find out the change in airflow velocity and pressure. Our findings revealed that small size particles will travel more with less inertia, whereas bigger size particles will travel less but with higher inertia at airflow rates of 30 L/min. It was found that dust deposition in the respiratory system determines the probability of inhalation and total deposition in the alveoli greatly varies with particle diameter size. An idealized URT model replicate patient-specific URT geometry which helped in finding real-time airflow velocity and pressure of dust particles. Particles with smaller diameter are capable of 100% deposition and inhalation rate at the alveoli, whereas particles with bigger diameter tend to deposit less and with lower inhalation rates at the alveoli.
{"title":"3D modeling of the airflow and aerosol deposition in the existence of dust","authors":"O. Altwijri, Razan Bakhshwin, E. H. Mirza, Y. A. Algabri, S. Chatpun, Ravish Javed","doi":"10.1166/mex.2023.2563","DOIUrl":"https://doi.org/10.1166/mex.2023.2563","url":null,"abstract":"Annually, more than two million deaths are caused due to the exposure of air pollution which cause damage to the lungs and the respiratory system. Topography, light-textured topsoil, drought, and desert climate make Saudi Arabia vulnerable to sand storms and dust. The study aims to\u0000 examine the deposition of dust particles in the human airway and its relationship to asthma and investigate the possibility that the dust deposition would be changed with different particle size diameters. An idealized upper respiratory tract 3D model was constructed with computer-aided design\u0000 software. Later a computational simulation was performed using computational fluid dynamics with 3 different sizes (0.03, 2, and 9 microns) of the dust particles to find out the change in airflow velocity and pressure. Our findings revealed that small size particles will travel more with less\u0000 inertia, whereas bigger size particles will travel less but with higher inertia at airflow rates of 30 L/min. It was found that dust deposition in the respiratory system determines the probability of inhalation and total deposition in the alveoli greatly varies with particle diameter size.\u0000 An idealized URT model replicate patient-specific URT geometry which helped in finding real-time airflow velocity and pressure of dust particles. Particles with smaller diameter are capable of 100% deposition and inhalation rate at the alveoli, whereas particles with bigger diameter tend to\u0000 deposit less and with lower inhalation rates at the alveoli.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"72 2","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138622864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abnormal activation of androgen receptor (AR) occurs in prostate cancer (PC) progression and metastasis. Lysine-specific demethylase 1 (LSD1), the first demethylating enzyme, regulates AR-mediated transcriptional activity. Considering the effect of Forkhead box protein A1 (FOXA1) on the expression of AR, estrogen receptor (ER) and tumor suppressor genes, this study investigated the demethylation of FOXA1 upon treatment with LSD1 inhibitors and assessed the biological behaviors of PC cells. PC cells were cultured and infected with viruses. After transient transfection, CWR22-RV1-Cas9 cells were selected by puromycin with expression of LSD1 detected by Western blot. Apart from measurement of formaldehyde production, immunoprecipitation and chromatin immunoprecipitation (ChIP) were performed, followed by ATAC-seq detection, and Western blot. The data indicated the association between LSD1-binding sites and high levels of FOXA1. LSD1 inhibitor treatment resulted in a dramatic decline in overall FOXA1 binding, significantly reducing chromosomal accessibility and also increasing lysine-methylated FOXA1 level, but it failed to affect H3K4me2 levels at LSD1-FOXA1 occupied sites. Overexpression of LSD1-WT obtained reverse outcome. Besides, LSD1 inhibition diminished binding of FOXA1 and restored lysine-methylation of FOXA1 in methylation-deficient cells with mutant K270R. Moreover, silencing of LSD1 suppressed CWR22-RV1 tumor growth, resulting in increased H3K4me2 and decreased AR-FL/V7 gene expression. K270me is demethylated by LSD1. LSD1 inhibitor disrupts FOXA1 chromatin association, blocks FOXA1 K270-demethylation and hinders AR binding, thereby suppressing PC cell growth.
{"title":"LSD1 inhibitor hinders the demethylation of FOXA1 to inhibit prostate cancer progression","authors":"Yongzhen Zhu, Lijia Ma, Wen Zhang, Xuelian Wu","doi":"10.1166/mex.2023.2567","DOIUrl":"https://doi.org/10.1166/mex.2023.2567","url":null,"abstract":"Abnormal activation of androgen receptor (AR) occurs in prostate cancer (PC) progression and metastasis. Lysine-specific demethylase 1 (LSD1), the first demethylating enzyme, regulates AR-mediated transcriptional activity. Considering the effect of Forkhead box protein A1 (FOXA1) on\u0000 the expression of AR, estrogen receptor (ER) and tumor suppressor genes, this study investigated the demethylation of FOXA1 upon treatment with LSD1 inhibitors and assessed the biological behaviors of PC cells. PC cells were cultured and infected with viruses. After transient transfection,\u0000 CWR22-RV1-Cas9 cells were selected by puromycin with expression of LSD1 detected by Western blot. Apart from measurement of formaldehyde production, immunoprecipitation and chromatin immunoprecipitation (ChIP) were performed, followed by ATAC-seq detection, and Western blot. The data indicated\u0000 the association between LSD1-binding sites and high levels of FOXA1. LSD1 inhibitor treatment resulted in a dramatic decline in overall FOXA1 binding, significantly reducing chromosomal accessibility and also increasing lysine-methylated FOXA1 level, but it failed to affect H3K4me2 levels\u0000 at LSD1-FOXA1 occupied sites. Overexpression of LSD1-WT obtained reverse outcome. Besides, LSD1 inhibition diminished binding of FOXA1 and restored lysine-methylation of FOXA1 in methylation-deficient cells with mutant K270R. Moreover, silencing of LSD1 suppressed CWR22-RV1 tumor growth, resulting\u0000 in increased H3K4me2 and decreased AR-FL/V7 gene expression. K270me is demethylated by LSD1. LSD1 inhibitor disrupts FOXA1 chromatin association, blocks FOXA1 K270-demethylation and hinders AR binding, thereby suppressing PC cell growth.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":" 8","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138611471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
It has been reported that, quercetin can improve body’s inflammatory response through Toll-like receptor 4/nuclear factor kappa-B (TLR4/NF-κB) signaling, thereby playing a role in treating acute appendicitis. Firstly, acute appendicitis model was established in rats, where blank group, acute appendicitis model group, and quercetin intervention group (quercetin group) were set up to detect the effect of quercetin on acute appendicitis. In addition, the model, Toll-like receptor 4 inhibitor (TAK-242), Lipopolysaccharides (LPS), quercetin+ TAK-242, and quercetin+LPS groups were set to assess whether quercetin reduces Caspase-1 (CASP-1) through TLR4/NF-KB signaling and regulates the release of inflammatory factors to exert the effect on treating acute appendicitis. (1) Quercetin significantly inhibited the biological effects of tissue inflammation in rats with appendicitis and this process was related to decreased expression of TLR4 and NF-κB; (2) Quercetin also inhibited TLR4 and NF-κB expression and after using TLR4 inhibitors, the release of inflammatory factors in rat appendix tissue was controlled, especially in the quercetin+TAK-242 group. Quercetin can significantly block the development of inflammatory response in acute appendicitis, and this process is mainly achieved by reducing inflammatory factors. In addition, quercetin can effectively inhibit TLR4/NF-κB signaling related to anti-inflammatory effect, thereby down-regulating the expression of CASP-1, and finally inhibiting the inflammatory response of acute appendicitis.
有报道称，槲皮素可通过toll样受体4/核因子κ b (TLR4/NF-κB)信号通路改善机体炎症反应，从而起到治疗急性阑尾炎的作用。首先，建立大鼠急性阑尾炎模型，分别建立空白组、急性阑尾炎模型组和槲皮素干预组(槲皮素组)，检测槲皮素对急性阑尾炎的影响。此外，建立模型，设置toll样受体4抑制剂(TAK-242)、脂多糖(LPS)、槲皮素+ TAK-242、槲皮素+LPS组，评估槲皮素是否通过TLR4/NF-KB信号通路降低Caspase-1 (CASP-1)，调节炎症因子的释放，从而发挥治疗急性阑尾炎的作用。(1)槲皮素显著抑制阑尾炎大鼠组织炎症的生物学效应，这一过程与降低TLR4和NF-κB的表达有关;(2)槲皮素还能抑制TLR4和NF-κB的表达，使用TLR4抑制剂后，大鼠阑尾组织中炎症因子的释放得到控制，尤其是槲皮素+TAK-242组。槲皮素可以显著阻断急性阑尾炎炎症反应的发展，这一过程主要是通过降低炎症因子来实现的。槲皮素还能有效抑制与抗炎作用相关的TLR4/NF-κB信号通路，从而下调CASP-1的表达，最终抑制急性阑尾炎的炎症反应。
{"title":"Effect of quercetin on acute appendicitis by reduction of Caspase-1 and inflammatory factors release through Toll-like receptor 4/nuclear factor kappa-B pathway","authors":"Gang Wang, Dan Han, Qingyu Zhang","doi":"10.1166/mex.2023.2551","DOIUrl":"https://doi.org/10.1166/mex.2023.2551","url":null,"abstract":"It has been reported that, quercetin can improve body’s inflammatory response through Toll-like receptor 4/nuclear factor kappa-B (TLR4/NF-κB) signaling, thereby playing a role in treating acute appendicitis. Firstly, acute appendicitis model was established in rats,\u0000 where blank group, acute appendicitis model group, and quercetin intervention group (quercetin group) were set up to detect the effect of quercetin on acute appendicitis. In addition, the model, Toll-like receptor 4 inhibitor (TAK-242), Lipopolysaccharides (LPS), quercetin+ TAK-242, and quercetin+LPS\u0000 groups were set to assess whether quercetin reduces Caspase-1 (CASP-1) through TLR4/NF-KB signaling and regulates the release of inflammatory factors to exert the effect on treating acute appendicitis. (1) Quercetin significantly inhibited the biological effects of tissue inflammation in rats\u0000 with appendicitis and this process was related to decreased expression of TLR4 and NF-κB; (2) Quercetin also inhibited TLR4 and NF-κB expression and after using TLR4 inhibitors, the release of inflammatory factors in rat appendix tissue was controlled, especially\u0000 in the quercetin+TAK-242 group. Quercetin can significantly block the development of inflammatory response in acute appendicitis, and this process is mainly achieved by reducing inflammatory factors. In addition, quercetin can effectively inhibit TLR4/NF-κB signaling related to\u0000 anti-inflammatory effect, thereby down-regulating the expression of CASP-1, and finally inhibiting the inflammatory response of acute appendicitis.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":" 30","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138613599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yuan Fang, Shichang Gao, Lei Wu, Jing Wang, Chao Yang, Lijun Tang, Pengwei Su, Qi Li
This study investigates the remediation of groundwater contaminated with heavy metals in overexploited areas using a modified approach involving the use of corn plant parts to produce biochar. The biochar was modified using a hydrothermal method, employing nanoscale zero-valent iron (nZVI) material to create a composite material for adsorbing heavy metals from water bodies. Adsorption experiments were conducted on the presence of Cr, Cu, and Zn ions in the water. The experimental investigations focused on the dosage of adsorption materials, solution pH, and stability of the adsorption material to validate the enhanced capability of the nanoscale zero-valent iron modified biochar composite (Fe-CBC-MO) for removing and adsorbing heavy metal ions (Cu, Cr, and Zn) from water. The results indicate that the adsorption capacity follows the sequence: Cr > Zn > Cu. Increasing the adsorbent dosage provides more adsorption sites, thereby improving the removal efficiency of heavy metals from water bodies. Considering cost-effectiveness, an optimal dosage of 0.15 g was selected. Under alkaline conditions, Cu and Zn ions precipitated significantly, leading to sustained high removal rates of heavy metals. Correspondingly, the rate constants were also relatively high. In acidic environments, the rate constant for Cr decreased significantly due to corrosion passivation. The composite material Fe-CBC-MO exhibited remarkable removal efficiency for all three heavy metals (Cr, Cu, Zn), demonstrating a strong capability for remediating heavy metal pollution.
{"title":"The role of nanoscale zero-valent iron (nZVI) in remediation of heavy metal contamination in groundwater overexploitation areas","authors":"Yuan Fang, Shichang Gao, Lei Wu, Jing Wang, Chao Yang, Lijun Tang, Pengwei Su, Qi Li","doi":"10.1166/mex.2023.2555","DOIUrl":"https://doi.org/10.1166/mex.2023.2555","url":null,"abstract":"This study investigates the remediation of groundwater contaminated with heavy metals in overexploited areas using a modified approach involving the use of corn plant parts to produce biochar. The biochar was modified using a hydrothermal method, employing nanoscale zero-valent iron\u0000 (nZVI) material to create a composite material for adsorbing heavy metals from water bodies. Adsorption experiments were conducted on the presence of Cr, Cu, and Zn ions in the water. The experimental investigations focused on the dosage of adsorption materials, solution pH, and stability\u0000 of the adsorption material to validate the enhanced capability of the nanoscale zero-valent iron modified biochar composite (Fe-CBC-MO) for removing and adsorbing heavy metal ions (Cu, Cr, and Zn) from water. The results indicate that the adsorption capacity follows the sequence: Cr > Zn\u0000 > Cu. Increasing the adsorbent dosage provides more adsorption sites, thereby improving the removal efficiency of heavy metals from water bodies. Considering cost-effectiveness, an optimal dosage of 0.15 g was selected. Under alkaline conditions, Cu and Zn ions precipitated significantly,\u0000 leading to sustained high removal rates of heavy metals. Correspondingly, the rate constants were also relatively high. In acidic environments, the rate constant for Cr decreased significantly due to corrosion passivation. The composite material Fe-CBC-MO exhibited remarkable removal efficiency\u0000 for all three heavy metals (Cr, Cu, Zn), demonstrating a strong capability for remediating heavy metal pollution.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":"51 4","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138622691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Caries is the most widespread oral disease threatening human health, especially early childhood caries, traditional treatment is a big challenge in clinical. In order to prevent early enamel demineralization, casein phosphopeptides (CPPs) incorporation with mesoporous 58S bioactive glass (M58S) was used to evaluate the remineralization enamel ability in vitro. By sol–gel method M58S was synthesized and showed good pore size and pore volume. In simulated oral fluid (SOF), 3%wt CPPs/M58S showed a better mineralized ability, remineralized layer was smooth and dense, thickness of 20 nm for 24 h, and structure of crystals was similar to the apatite, Human oral adhesion keratinocytes cells (HOKs) can regulate expression and promoted cell proliferation and differentiation, which showed a good biocompatibility. It is expected that CPPs/M58S may be an effective agent in the treatment of early children caries and dental allergies.
{"title":"Effect of casein phosphopeptide incorporation with mesoporous 58S bioactive glass on remineralization in vitro for teeth enamel regeneration","authors":"Lu Tang, Yu Li, Suqi Wang, Jin Yang, Zhihong Dong","doi":"10.1166/mex.2023.2544","DOIUrl":"https://doi.org/10.1166/mex.2023.2544","url":null,"abstract":"Caries is the most widespread oral disease threatening human health, especially early childhood caries, traditional treatment is a big challenge in clinical. In order to prevent early enamel demineralization, casein phosphopeptides (CPPs) incorporation with mesoporous 58S bioactive\u0000 glass (M58S) was used to evaluate the remineralization enamel ability in vitro. By sol–gel method M58S was synthesized and showed good pore size and pore volume. In simulated oral fluid (SOF), 3%wt CPPs/M58S showed a better mineralized ability, remineralized layer was smooth and\u0000 dense, thickness of 20 nm for 24 h, and structure of crystals was similar to the apatite, Human oral adhesion keratinocytes cells (HOKs) can regulate expression and promoted cell proliferation and differentiation, which showed a good biocompatibility. It is expected that CPPs/M58S may be an\u0000 effective agent in the treatment of early children caries and dental allergies.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":" August","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138611349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Osteoprotegerin (OPG), as one of the tumor necrosis factor receptors, is believed to be related with Osteoprotegerin (OP) and arteriosclerosis (AS). This study aims to explore the effect of OPG on osteoblasts (OB) and AS. The nanocomplex Poly(ethylene glycol)-Poly(L-Lysine)-Osteoprotegerin (PPO) was prepared by introducing OPG plasmid to Poly(ethylene glycol)-Poly(L-Lysine). Healthy group, AS group, AS combined with OP group were designed to measure apoptosis, activity, adhesion, and calcification of OB in Polycaprolactone (PCL) by flow cytometry, MTT method, alizarin red staining, scanning electron microscope and other methods. The effect of PPO on bone mineral density and arteriosclerosis of ApoE−/−/RANKL+/+ mice was observed. Bone Mineral Density (BMD) was positively correlated with ABI while negatively correlated with pulse wave velocity. OPG in AS combined with OP group was higher than healthy group, and AS group was also higher than healthy group. PPO-administered mice had dense bone trabeculae and higher bone density while the control group was the opposite. The effect of PPO on the stable expression of OPG in mice reduced the plaque area and the degree of vascular calcification. PPO can enhance OB activity in vitro, inhibit cell apoptosis, promote cell calcification and PCL adhesion, decrease the area of atherosclerotic plaque and calcification, and increase the BMD of the femoral neck. PPO can promote the adhesion and calcification of MC3T3-E1 on PCL, which is of great significance for maintaining sufficient bone strength and reducing blood calcium. In addition, PPO compound drugs can increase bone density, reduce arterial plaque area and vascular calcification.
{"title":"The effect of osteoprotegerin nanocomplex on osteoprotegerin and arteriosclerosis in mice","authors":"Zhiwen He, Shuang Wang, Xiaoxuan Xia","doi":"10.1166/mex.2023.2573","DOIUrl":"https://doi.org/10.1166/mex.2023.2573","url":null,"abstract":"Osteoprotegerin (OPG), as one of the tumor necrosis factor receptors, is believed to be related with Osteoprotegerin (OP) and arteriosclerosis (AS). This study aims to explore the effect of OPG on osteoblasts (OB) and AS. The nanocomplex Poly(ethylene glycol)-Poly(L-Lysine)-Osteoprotegerin\u0000 (PPO) was prepared by introducing OPG plasmid to Poly(ethylene glycol)-Poly(L-Lysine). Healthy group, AS group, AS combined with OP group were designed to measure apoptosis, activity, adhesion, and calcification of OB in Polycaprolactone (PCL) by flow cytometry, MTT method, alizarin red staining,\u0000 scanning electron microscope and other methods. The effect of PPO on bone mineral density and arteriosclerosis of ApoE−/−/RANKL+/+ mice was observed. Bone Mineral Density (BMD) was positively correlated with ABI while negatively correlated with pulse wave\u0000 velocity. OPG in AS combined with OP group was higher than healthy group, and AS group was also higher than healthy group. PPO-administered mice had dense bone trabeculae and higher bone density while the control group was the opposite. The effect of PPO on the stable expression of OPG in\u0000 mice reduced the plaque area and the degree of vascular calcification. PPO can enhance OB activity in vitro, inhibit cell apoptosis, promote cell calcification and PCL adhesion, decrease the area of atherosclerotic plaque and calcification, and increase the BMD of the femoral neck.\u0000 PPO can promote the adhesion and calcification of MC3T3-E1 on PCL, which is of great significance for maintaining sufficient bone strength and reducing blood calcium. In addition, PPO compound drugs can increase bone density, reduce arterial plaque area and vascular calcification.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":" 15","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138615893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
With the increasing popularity of competitive sports, martial arts routines have attracted great attention. However, myocardial ischemic injury is one of the most common sports-related diseases in martial arts routines. In this study, γ-Fe2O3 nanoparticles were used as a treatment for myocardial injury. The safety of these nanoparticles was determined by assessing apoptosis rates and reactive oxygen species levels in myocardial cells, as well as biochemical indicators such as lactate dehydrogenase and superoxide dismutase. The results showed that the aforementioned indicators were normal, indicating that γ-Fe2O3 nanoparticles are safe for myocardial cells. Additionally, the application of γ-Fe2O3 nanoparticles to hypoxic environments significantly improved anti-hypoxia ability, alleviated calcium overload in myocardial cells, and reduced intracellular reactive oxygen species content, thereby protecting myocardial cells from hypoxia damage. Therefore, this study provides an effective strategy for treating ischemic injury in martial arts athletes and establishes an experimental foundation for the clinical management of myocardial ischemic injury.
{"title":"Preparation and application of amphiphilic polymer nanomaterials for ischemic injury","authors":"Jiayu Li, Yili Xu","doi":"10.1166/mex.2023.2557","DOIUrl":"https://doi.org/10.1166/mex.2023.2557","url":null,"abstract":"With the increasing popularity of competitive sports, martial arts routines have attracted great attention. However, myocardial ischemic injury is one of the most common sports-related diseases in martial arts routines. In this study, γ-Fe2O3 nanoparticles\u0000 were used as a treatment for myocardial injury. The safety of these nanoparticles was determined by assessing apoptosis rates and reactive oxygen species levels in myocardial cells, as well as biochemical indicators such as lactate dehydrogenase and superoxide dismutase. The results showed\u0000 that the aforementioned indicators were normal, indicating that γ-Fe2O3 nanoparticles are safe for myocardial cells. Additionally, the application of γ-Fe2O3 nanoparticles to hypoxic environments significantly improved anti-hypoxia\u0000 ability, alleviated calcium overload in myocardial cells, and reduced intracellular reactive oxygen species content, thereby protecting myocardial cells from hypoxia damage. Therefore, this study provides an effective strategy for treating ischemic injury in martial arts athletes and establishes\u0000 an experimental foundation for the clinical management of myocardial ischemic injury.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":" 46","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138617587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Most gastric cancer patients have cancer cell metastasis at the time of being diagnosed. Cisplatin chemotherapy can slow down the development of gastric cancer, but the drug resistance will develop after a long time of chemotherapy. Previous studies have found that quercetin improves resistance of chemotherapy drugs. Therefore, this study intends to explore quercetin’s role in gastric cancer. SGC-7901 drug-resistant cell line was cultured and intervened. MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay assessed cell proliferation, cell survival rate, IC50 value and sensitivity along with analysis of cell apoptosis, proliferation by colony formation assay and qRT-PCR (real-time reverse transcription-PCR) and Western blot detection of FOXD3 (Forkhead box D3) levels. Gastric cancer xenograft tumor mouse model was established to assess its in vivo role. The drug-resistant cell model of gastric cancer was successfully constructed and quercetin inhibited cell survival to a certain extent and improved its chemosensitivity. The pro-apoptotic effect of quercetin on cisplatin chemotherapy resistance in gastric cancer is related to the increased FOXD3 level. Quercetin can directly regulate the expression of FOXD3, which is an activation effect. The inhibition rate of gastric cancer mice in vivo was the most prominent in the quercetin+drug resistance group. The tumor-bearing site was significantly reduced and the number of surviving mice was the highest, whose tumor volume was consistently lower than that of other groups. Conclusively, quercetin has a strong anti-tumor effect. It can inhibit gastric cancer cell activity and accelerate apoptosis by activating FOXD3 signaling pathway.
{"title":"The inhibitory effect of quercetin on chemotherapeutic drug resistance of gastric cancer through forkhead box D3 signaling pathway","authors":"Liqian Chang, Yuan Gao, Na An","doi":"10.1166/mex.2023.2558","DOIUrl":"https://doi.org/10.1166/mex.2023.2558","url":null,"abstract":"Most gastric cancer patients have cancer cell metastasis at the time of being diagnosed. Cisplatin chemotherapy can slow down the development of gastric cancer, but the drug resistance will develop after a long time of chemotherapy. Previous studies have found that quercetin improves\u0000 resistance of chemotherapy drugs. Therefore, this study intends to explore quercetin’s role in gastric cancer. SGC-7901 drug-resistant cell line was cultured and intervened. MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay assessed cell proliferation, cell survival\u0000 rate, IC50 value and sensitivity along with analysis of cell apoptosis, proliferation by colony formation assay and qRT-PCR (real-time reverse transcription-PCR) and Western blot detection of FOXD3 (Forkhead box D3) levels. Gastric cancer xenograft tumor mouse model was established to assess\u0000 its in vivo role. The drug-resistant cell model of gastric cancer was successfully constructed and quercetin inhibited cell survival to a certain extent and improved its chemosensitivity. The pro-apoptotic effect of quercetin on cisplatin chemotherapy resistance in gastric cancer is\u0000 related to the increased FOXD3 level. Quercetin can directly regulate the expression of FOXD3, which is an activation effect. The inhibition rate of gastric cancer mice in vivo was the most prominent in the quercetin+drug resistance group. The tumor-bearing site was significantly reduced\u0000 and the number of surviving mice was the highest, whose tumor volume was consistently lower than that of other groups. Conclusively, quercetin has a strong anti-tumor effect. It can inhibit gastric cancer cell activity and accelerate apoptosis by activating FOXD3 signaling pathway.","PeriodicalId":18318,"journal":{"name":"Materials Express","volume":" 2","pages":""},"PeriodicalIF":0.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138614588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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