{"title":"Erratum: Study the Profile of Some Antioxidant Markers in Diabetic Mellitus and Non-diabetic Patients with Cardiovascular Disease","authors":"","doi":"10.4103/MJBL.Erratum_2","DOIUrl":"https://doi.org/10.4103/MJBL.Erratum_2","url":null,"abstract":"","PeriodicalId":18326,"journal":{"name":"Medical Journal of Babylon","volume":"20 1","pages":"220 - 220"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44798492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.4103/MJBL.MJBL_294_22
Balqees Hasan, Wasan H Saud
Background: Disturbances induced by chelating agents in the essential metal ions, including zinc and copper, can adversely impact the health of patients with transfusion-dependent thalassemia (TDT). Objectives: The aim of this study was to investigate the effect of different iron chelators on the levels of zinc and copper among patients with TDT. Materials and Methods: This case-control study involved 94 patients with TDT and 59 healthy controls, their ages ranged from 5 to 37 years. Patients with TDT were subdivided into two groups: 51 patients were on deferasirox and 43 were on deferoxamine. Blood samples were collected from all participants for complete blood counts and serum ferritin, iron, zinc, and copper levels, which were determined by spectrophotometry. Results: Serum iron, ferritin, and zinc levels were significantly higher among patients with TDT, while the Hb level was significantly lower than that in the healthy population (P < 0.05). Serum copper levels did not differ significantly between the groups. A high frequency of zinc deficiency was reported among patients with TDT on deferasirox (56.9%) and control group (47.5%), compared to 16.3% for patients with TDT on deferoxamine (P < 0.001). For serum copper, most of them had normal levels (81.4%–90.2%). Patients on deferoxamine had significantly higher serum zinc and copper levels than those on deferasirox and control group, although the mean serum values were within the normal range of values for the three groups (P < 0.01). Conclusions: Low serum zinc was reported in a considerable percentage of patients with TDT receiving deferasirox compared to those receiving deferoxamine, while serum copper was within normal range in the majority of patients with TDT.
{"title":"Zinc and copper in transfusion-dependent thalassemia patients on different iron chelators in Basrah: A case-control study","authors":"Balqees Hasan, Wasan H Saud","doi":"10.4103/MJBL.MJBL_294_22","DOIUrl":"https://doi.org/10.4103/MJBL.MJBL_294_22","url":null,"abstract":"Background: Disturbances induced by chelating agents in the essential metal ions, including zinc and copper, can adversely impact the health of patients with transfusion-dependent thalassemia (TDT). Objectives: The aim of this study was to investigate the effect of different iron chelators on the levels of zinc and copper among patients with TDT. Materials and Methods: This case-control study involved 94 patients with TDT and 59 healthy controls, their ages ranged from 5 to 37 years. Patients with TDT were subdivided into two groups: 51 patients were on deferasirox and 43 were on deferoxamine. Blood samples were collected from all participants for complete blood counts and serum ferritin, iron, zinc, and copper levels, which were determined by spectrophotometry. Results: Serum iron, ferritin, and zinc levels were significantly higher among patients with TDT, while the Hb level was significantly lower than that in the healthy population (P < 0.05). Serum copper levels did not differ significantly between the groups. A high frequency of zinc deficiency was reported among patients with TDT on deferasirox (56.9%) and control group (47.5%), compared to 16.3% for patients with TDT on deferoxamine (P < 0.001). For serum copper, most of them had normal levels (81.4%–90.2%). Patients on deferoxamine had significantly higher serum zinc and copper levels than those on deferasirox and control group, although the mean serum values were within the normal range of values for the three groups (P < 0.01). Conclusions: Low serum zinc was reported in a considerable percentage of patients with TDT receiving deferasirox compared to those receiving deferoxamine, while serum copper was within normal range in the majority of patients with TDT.","PeriodicalId":18326,"journal":{"name":"Medical Journal of Babylon","volume":"20 1","pages":"124 - 129"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48423242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.4103/MJBL.MJBL_255_22
Zahraa Abdulhakeem, A. Odda, S. Abdulsattar
Background: Diabetes mellitus (DM) is a metabolic disorder known as hyperglycemia, which is brought on by impaired insulin secretion, inefficient insulin utilization, or both. Objectives: This study’s aim was to assess the lipase and amylase amylase pancreatic enzymes levels in sera of (T2DM) patients and healthy control subjects. Materials and Methods: A case-control research was conducted from December 2021 to March 2022 at the Hilla District Center for Diabetes and Endocrinology in Babylon, Iraq.It included a group of 39 patients with type 2 DM and 39 healthy people between the ages of 35 to 60 years. Results: Results were indicating a significant difference in FBS among groups, The mean levels of FBS for DM was (203.7 ± 72.3) mg/dL which was significantly greater than it was for the control group (88.6 ± 7.64) mg/dL,(P ≤ 0.001). The mean of HbA1c for DM (9.5 ± 2.35) was significantly higher than for control (4.9 ± 0.48), P ≤ 0.001.The mean levels of the Amylase and Lipase activity in the DM patients group were (71.4 ± 29.11) and (49.7 ± 14.69) (U/L). When compared to the control group’s mean values, which were (72.7 ± 27.19) and (42.0 ± 7.35) (U/L). The mean levels of Lipase activity were shown a significant difference in T2DM patients compared to the healthy control group, (P < 0.05). Conclusion: Increased serum level of lipase without any symptoms of pancreatitis was indicated in type II diabetes mellitus patients.
{"title":"Relationship of serum ghrelin, amylase and lipase with insulin level in type 2 diabetes mellitus patients","authors":"Zahraa Abdulhakeem, A. Odda, S. Abdulsattar","doi":"10.4103/MJBL.MJBL_255_22","DOIUrl":"https://doi.org/10.4103/MJBL.MJBL_255_22","url":null,"abstract":"Background: Diabetes mellitus (DM) is a metabolic disorder known as hyperglycemia, which is brought on by impaired insulin secretion, inefficient insulin utilization, or both. Objectives: This study’s aim was to assess the lipase and amylase amylase pancreatic enzymes levels in sera of (T2DM) patients and healthy control subjects. Materials and Methods: A case-control research was conducted from December 2021 to March 2022 at the Hilla District Center for Diabetes and Endocrinology in Babylon, Iraq.It included a group of 39 patients with type 2 DM and 39 healthy people between the ages of 35 to 60 years. Results: Results were indicating a significant difference in FBS among groups, The mean levels of FBS for DM was (203.7 ± 72.3) mg/dL which was significantly greater than it was for the control group (88.6 ± 7.64) mg/dL,(P ≤ 0.001). The mean of HbA1c for DM (9.5 ± 2.35) was significantly higher than for control (4.9 ± 0.48), P ≤ 0.001.The mean levels of the Amylase and Lipase activity in the DM patients group were (71.4 ± 29.11) and (49.7 ± 14.69) (U/L). When compared to the control group’s mean values, which were (72.7 ± 27.19) and (42.0 ± 7.35) (U/L). The mean levels of Lipase activity were shown a significant difference in T2DM patients compared to the healthy control group, (P < 0.05). Conclusion: Increased serum level of lipase without any symptoms of pancreatitis was indicated in type II diabetes mellitus patients.","PeriodicalId":18326,"journal":{"name":"Medical Journal of Babylon","volume":"20 1","pages":"71 - 76"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47949293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Erratum: Assessment of Electroencephalographic Changes and Clinical Characteristics among Patients with Substance-related Disorder","authors":"","doi":"10.4103/MJBL.Erratum_3","DOIUrl":"https://doi.org/10.4103/MJBL.Erratum_3","url":null,"abstract":"","PeriodicalId":18326,"journal":{"name":"Medical Journal of Babylon","volume":"20 1","pages":"221 - 221"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47829754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.4103/mjbl.mjbl_314_23
SalimShamkhi Jaafar, HasanainKhaleel Shareef
{"title":"Association between single nucleotide polymorphisms of F3 gene (rs12029080 T>G and rs11165176 C>T) with thrombosis in SARS-CoV-2 patients in Babylon Province","authors":"SalimShamkhi Jaafar, HasanainKhaleel Shareef","doi":"10.4103/mjbl.mjbl_314_23","DOIUrl":"https://doi.org/10.4103/mjbl.mjbl_314_23","url":null,"abstract":"","PeriodicalId":18326,"journal":{"name":"Medical Journal of Babylon","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135949753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.4103/MJBL.MJBL_243_22
Aseel AL-Bairmani, H. Al-masoudi
Background: Children are most frequently infected with Enterobius vermicularis, a human-pathogenic intestinal parasite that belongs to the nematodes and causes enterobiasis. Objectives: Evaluation of interleukin (IL)-1B and IL-2 levels in pinworm-infected youngsters is the goal. Materials and Methods: A total of 87 children—including 30 control subjects—participated in this study. Their ages ranged from 2 to 13 years old, and both sexes (50 females and 37 males) were represented. IL-1B, IL-2, and IL-10 levels were measured using an enzyme-linked immunosorbent assay test. Results: When using the cellophane tape method, the results showed that 53 (60.92%) clinically infected patients had laboratory examinations, whereas all 87 (100.0%) clinically infected patients had positive diagnostic results. Additionally, the level of proinflammatory cytokines (IL-1B and IL-2) was slightly higher in the intestinal E. vermicularis, a human-pathogenic organism shown to be somewhat more prevalent in patients than in the control group in this investigation. Thirty children participated in the study. Additionally, the level of IL-10 did not increase much (P = 0.005). Conclusion: There was increasing serum of IL-1B, IL-2, and IL-10 in patients with enterobiasis.
{"title":"Assessment of the serum level of IL-1B, IL-2, and IL-10 in children infected with Enterobius vermicularis in Babylon province","authors":"Aseel AL-Bairmani, H. Al-masoudi","doi":"10.4103/MJBL.MJBL_243_22","DOIUrl":"https://doi.org/10.4103/MJBL.MJBL_243_22","url":null,"abstract":"Background: Children are most frequently infected with Enterobius vermicularis, a human-pathogenic intestinal parasite that belongs to the nematodes and causes enterobiasis. Objectives: Evaluation of interleukin (IL)-1B and IL-2 levels in pinworm-infected youngsters is the goal. Materials and Methods: A total of 87 children—including 30 control subjects—participated in this study. Their ages ranged from 2 to 13 years old, and both sexes (50 females and 37 males) were represented. IL-1B, IL-2, and IL-10 levels were measured using an enzyme-linked immunosorbent assay test. Results: When using the cellophane tape method, the results showed that 53 (60.92%) clinically infected patients had laboratory examinations, whereas all 87 (100.0%) clinically infected patients had positive diagnostic results. Additionally, the level of proinflammatory cytokines (IL-1B and IL-2) was slightly higher in the intestinal E. vermicularis, a human-pathogenic organism shown to be somewhat more prevalent in patients than in the control group in this investigation. Thirty children participated in the study. Additionally, the level of IL-10 did not increase much (P = 0.005). Conclusion: There was increasing serum of IL-1B, IL-2, and IL-10 in patients with enterobiasis.","PeriodicalId":18326,"journal":{"name":"Medical Journal of Babylon","volume":"20 1","pages":"59 - 63"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47374799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.4103/MJBL.MJBL_287_22
R. Naji, R. AL-Daher, D. Abood, K. Madlum
Background: Plants are the source of many important drugs. Ricinus communis is utilized as a traditional folkloric remedy for the treatment of a wide extend of diseases around the world. Large number of phytochemicals such as alkaloids, terpenes, and phenolic compounds that have an established anticancer, antimicrobial, and immunological impact presence in many plant families. Objective: The aim of the present study was to investigate the immunological impact of R. communis leaves extract on human immune response using isolated human peripheral blood mononuclear cells (PBMNC). Materials and Methods: Cells were isolated using the gradient centrifugation method and treated with R. communis leaves extract at different concentrations, and then, the cytotoxic effect was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and the immunologic effect was estimated by measuring the cytokine levels via the enzyme-linked immunosorbent assay technique. Results: Plant extract showed a low cytotoxic effect on PBMNC at therapeutic doses and high doses (1000 µg/mL) used. Plant extract caused a significant reduction in the levels of interleukin (IL)-4 and IL-17 at higher doses and increased IFN-ɣ at both doses used. Conclusion: R. communis leaves extract appears to be safe for medical uses and has immunomodulatory effects.
{"title":"Immunological impact of Ricinus communis leaves extract on isolated human peripheral blood mononuclear cells","authors":"R. Naji, R. AL-Daher, D. Abood, K. Madlum","doi":"10.4103/MJBL.MJBL_287_22","DOIUrl":"https://doi.org/10.4103/MJBL.MJBL_287_22","url":null,"abstract":"Background: Plants are the source of many important drugs. Ricinus communis is utilized as a traditional folkloric remedy for the treatment of a wide extend of diseases around the world. Large number of phytochemicals such as alkaloids, terpenes, and phenolic compounds that have an established anticancer, antimicrobial, and immunological impact presence in many plant families. Objective: The aim of the present study was to investigate the immunological impact of R. communis leaves extract on human immune response using isolated human peripheral blood mononuclear cells (PBMNC). Materials and Methods: Cells were isolated using the gradient centrifugation method and treated with R. communis leaves extract at different concentrations, and then, the cytotoxic effect was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and the immunologic effect was estimated by measuring the cytokine levels via the enzyme-linked immunosorbent assay technique. Results: Plant extract showed a low cytotoxic effect on PBMNC at therapeutic doses and high doses (1000 µg/mL) used. Plant extract caused a significant reduction in the levels of interleukin (IL)-4 and IL-17 at higher doses and increased IFN-ɣ at both doses used. Conclusion: R. communis leaves extract appears to be safe for medical uses and has immunomodulatory effects.","PeriodicalId":18326,"journal":{"name":"Medical Journal of Babylon","volume":"20 1","pages":"120 - 123"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43086874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.4103/MJBL.MJBL_307_22
A. Alahmar, Aqeel Al Jothery, Q. AL-Daami, A. Abbas, A. Al-Hassnawi
Background: Oxidative stress is a contributing factor in 30%–80% of infertile men. Objective: The aim of this study was to investigate the impact of coenzyme Q10 on dexamethasone-induced oxidative stress in rats testes. Materials and Methods: Sixteen male Wistar rats were divided into four groups of four: group 1 (control) received 1 mL of distilled water daily orally; group 2 received dexamethasone 0.2 mg/kg/day intraperitoneally; group 3 received coenzyme Q10 30 mg/kg/day orally; and group 4 received dexamethasone and coenzyme Q10 via the same routes. Testicular total antioxidant capacity (TAC), superoxide dismutase (SOD), and catalase (CAT) activities as well as testicular histopathological changes were compared among groups. Results: Although testicular SOD was lower in groups 3 and 4, dexamethasone and coenzyme Q10 treatment did not significantly alter TAC, SOD, CAT, or histopathological features of rats testes. Conclusion: Dexamethasone (0.2 mg/kg/day) and coenzyme Q10 (30 mg/kg/day) may have no impact on gonadal oxidative stress, antioxidant capacity, or spermatogenesis in rats testes with these doses.
{"title":"The effect of coenzyme Q10 on dexamethasone-induced oxidative stress in rats testes","authors":"A. Alahmar, Aqeel Al Jothery, Q. AL-Daami, A. Abbas, A. Al-Hassnawi","doi":"10.4103/MJBL.MJBL_307_22","DOIUrl":"https://doi.org/10.4103/MJBL.MJBL_307_22","url":null,"abstract":"Background: Oxidative stress is a contributing factor in 30%–80% of infertile men. Objective: The aim of this study was to investigate the impact of coenzyme Q10 on dexamethasone-induced oxidative stress in rats testes. Materials and Methods: Sixteen male Wistar rats were divided into four groups of four: group 1 (control) received 1 mL of distilled water daily orally; group 2 received dexamethasone 0.2 mg/kg/day intraperitoneally; group 3 received coenzyme Q10 30 mg/kg/day orally; and group 4 received dexamethasone and coenzyme Q10 via the same routes. Testicular total antioxidant capacity (TAC), superoxide dismutase (SOD), and catalase (CAT) activities as well as testicular histopathological changes were compared among groups. Results: Although testicular SOD was lower in groups 3 and 4, dexamethasone and coenzyme Q10 treatment did not significantly alter TAC, SOD, CAT, or histopathological features of rats testes. Conclusion: Dexamethasone (0.2 mg/kg/day) and coenzyme Q10 (30 mg/kg/day) may have no impact on gonadal oxidative stress, antioxidant capacity, or spermatogenesis in rats testes with these doses.","PeriodicalId":18326,"journal":{"name":"Medical Journal of Babylon","volume":"20 1","pages":"130 - 135"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48568202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.4103/MJBL.MJBL_256_22
A. Salman, Zinah Ali, N. Selman
Background: The most prevalent disease in the world, is chronic hepatitis B virus (CHB). It has several causes like, drugs, alcohol consumption, toxicity and auto-immune disease, but in most cases it is caused by hepatitis viruses (viral hepatitis). Hepcidin regulates the body iron negatively by binding to the cellular iron exporter ferroportin in target cells, like macrophages, enterocytes and making its internalization and deprivation, thus reducing iron efflux into plasma causing decrease serum iron level and an elevated in intracellular iron. Materials and Methods: One hundred subjects; 50 diagnosed with chronic hepatitis B virus and 50 healthy subjects were enrolled in this study. Age ranged between (20–60) years (for patients and control). Hepcidin and ferritin level in serum were estimated by enzyme- linked immunosorbent assay (ELISA) technique, while serum iron concentration was calculated by the Siemens Dimension by Direct method (Ferene). Results: In chronic hepatitis B virus group serum hepcidin, ferritin and iron levels significantly elevated compared with control group (P < 0.01). On the other hand, the current study observed significant (p value <0.05) positive correlation for hepcidin with ferritin and iron in (CHB) patients. Conclusion: Among chronic hepatitis B patients in Babylon province, increase the level of some iron regulatory parameters; indicate the significant linked with the progression of chronic hepatitis B virus.
{"title":"Hepcidin level in sera of patients with chronic hepatitis B virus in Babylon province","authors":"A. Salman, Zinah Ali, N. Selman","doi":"10.4103/MJBL.MJBL_256_22","DOIUrl":"https://doi.org/10.4103/MJBL.MJBL_256_22","url":null,"abstract":"Background: The most prevalent disease in the world, is chronic hepatitis B virus (CHB). It has several causes like, drugs, alcohol consumption, toxicity and auto-immune disease, but in most cases it is caused by hepatitis viruses (viral hepatitis). Hepcidin regulates the body iron negatively by binding to the cellular iron exporter ferroportin in target cells, like macrophages, enterocytes and making its internalization and deprivation, thus reducing iron efflux into plasma causing decrease serum iron level and an elevated in intracellular iron. Materials and Methods: One hundred subjects; 50 diagnosed with chronic hepatitis B virus and 50 healthy subjects were enrolled in this study. Age ranged between (20–60) years (for patients and control). Hepcidin and ferritin level in serum were estimated by enzyme- linked immunosorbent assay (ELISA) technique, while serum iron concentration was calculated by the Siemens Dimension by Direct method (Ferene). Results: In chronic hepatitis B virus group serum hepcidin, ferritin and iron levels significantly elevated compared with control group (P < 0.01). On the other hand, the current study observed significant (p value <0.05) positive correlation for hepcidin with ferritin and iron in (CHB) patients. Conclusion: Among chronic hepatitis B patients in Babylon province, increase the level of some iron regulatory parameters; indicate the significant linked with the progression of chronic hepatitis B virus.","PeriodicalId":18326,"journal":{"name":"Medical Journal of Babylon","volume":"20 1","pages":"77 - 80"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42741122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.4103/MJBL.MJBL_326_22
Sarah Hasan Al-khafaji, Zainab Al-mahdi, Wisam W. Alhamadi
Background: Crossbite is any discrepancy in the relationship between the upper and lower teeth. Crossbites have a relationship with oral microorganisms, such as S. mutans and lactobacillus infections. Viral infections can spread to the oral cavity especially its soft tissue especially herpes simplex virus & cytomegalovirus. Secretory immunoglobulin represents a factor for acquired immunity in the oral cavity which is associated with oral infection and variation. Objectives: Isolation of microbes from crossbite patients, identifying by molecular techniques, determining sIgA level, and selecting appropriate antibiotics for the most common microbe. Materials and Methods: A total of 60 (30 samples of saliva and swab before Orthodontic Treatment and 30 samples of saliva after Orthodontic) during the period from October 2021 to April 2022, Sample were taken from patients and microbiological identification by microscopical and biochemical tests. An antibiotic sensitivity test for S. mutans. Genetic identification was detected in the S. mutans samples by using specificity Sm479 primers, and HCMV primers for cytomegalovirus. Secretory IgA was determined by ELISA Kit. Results: A number of gram-positive bacteria were more than gram-negative (51% and 49%) respectively. The S. mutans and Lactobacillus are the most frequent among the other species, followed by Candida, Klebsiella, and S. epidermidis. 53% in male, while 47% in female, and age group (1315) was the most prevalent among samples 53%. Regarding S. mutans, the highest sensitive rate is Erythromycin 81%, followed by Amoxiclav 57%, Vancomycin 52%, and Nalidixic acid 43%. Conclusion: Most infectious cases were in ages (14-15) years. S. mutans is more prevalent among bacterial infections, molecular detection by PCR more specifically for S.mutans and CMV virus, sIgA level non-significant inverse correlations between age and sIgA level before treatment, while significant inverse correlation between age and sIgA level after treatment, and S. mutans, reported the highest sensitive rate is Erythromycin (81%), followed by Amoxiclav, Vancomycin,and Nalidixic acid.
{"title":"Microbial distribution and secretory IgA level among crossbite patients at an early stage of comprehensive orthodontic treatment","authors":"Sarah Hasan Al-khafaji, Zainab Al-mahdi, Wisam W. Alhamadi","doi":"10.4103/MJBL.MJBL_326_22","DOIUrl":"https://doi.org/10.4103/MJBL.MJBL_326_22","url":null,"abstract":"Background: Crossbite is any discrepancy in the relationship between the upper and lower teeth. Crossbites have a relationship with oral microorganisms, such as S. mutans and lactobacillus infections. Viral infections can spread to the oral cavity especially its soft tissue especially herpes simplex virus & cytomegalovirus. Secretory immunoglobulin represents a factor for acquired immunity in the oral cavity which is associated with oral infection and variation. Objectives: Isolation of microbes from crossbite patients, identifying by molecular techniques, determining sIgA level, and selecting appropriate antibiotics for the most common microbe. Materials and Methods: A total of 60 (30 samples of saliva and swab before Orthodontic Treatment and 30 samples of saliva after Orthodontic) during the period from October 2021 to April 2022, Sample were taken from patients and microbiological identification by microscopical and biochemical tests. An antibiotic sensitivity test for S. mutans. Genetic identification was detected in the S. mutans samples by using specificity Sm479 primers, and HCMV primers for cytomegalovirus. Secretory IgA was determined by ELISA Kit. Results: A number of gram-positive bacteria were more than gram-negative (51% and 49%) respectively. The S. mutans and Lactobacillus are the most frequent among the other species, followed by Candida, Klebsiella, and S. epidermidis. 53% in male, while 47% in female, and age group (1315) was the most prevalent among samples 53%. Regarding S. mutans, the highest sensitive rate is Erythromycin 81%, followed by Amoxiclav 57%, Vancomycin 52%, and Nalidixic acid 43%. Conclusion: Most infectious cases were in ages (14-15) years. S. mutans is more prevalent among bacterial infections, molecular detection by PCR more specifically for S.mutans and CMV virus, sIgA level non-significant inverse correlations between age and sIgA level before treatment, while significant inverse correlation between age and sIgA level after treatment, and S. mutans, reported the highest sensitive rate is Erythromycin (81%), followed by Amoxiclav, Vancomycin,and Nalidixic acid.","PeriodicalId":18326,"journal":{"name":"Medical Journal of Babylon","volume":"20 1","pages":"160 - 167"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47983418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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