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Fabry-Pérot Microscopy for Improved Contrast Enhancement and Quantitative Phase Imaging 用于改进对比度增强和定量相位成像的法布里-帕姆罗显微术。
IF 2 3区 物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS Pub Date : 2026-01-18 DOI: 10.1002/jbio.70217
Johannes Pittrich, Georg von Köller, Christoph Dillitzer, Daniel Sandner, Ellen Emken, Julia Sistermanns, Zsuzsanna Wolf, Martin Schlegel, Gregor Weirich, Reinhard Kienberger, Oliver Hayden, Hristo Iglev

Fabry-Pérot microscopy (FPM) is a label-free imaging technique that combines a microfluidic optical cavity with a tunable light source to enhance contrast and enable quantitative phase imaging. It integrates a fixed-length Fabry-Pérot cavity into a coated microfluidic channel and utilizes resonance-based interference to selectively highlight structures with specific optical thickness. This design preserves lateral resolution while providing > 20-fold contrast enhancement compared to conventional wide-field microscopy. Demonstrations with human oral epithelial cells, blood components, and E. coli show clear visualization of subcellular features and differentiation of cell types. Notably, FPM achieves imaging through spectral tuning rather than mechanical scanning, ensuring faster and more stable operation. The system is compatible with standard microscope optics and works under both static and flow conditions, offering significant potential for high-throughput cytometry, biological research, and in vitro diagnostics. These results establish FPM as a versatile extension of wide-field microscopy, enabling contrast-tunable, quantitative imaging of biomedical samples.

FPM是一种无标签成像技术,它将微流体光学腔与可调光源相结合,以增强对比度并实现定量相位成像。它将固定长度的法布里-帕姆罗特腔集成到涂层微流体通道中,并利用基于共振的干涉来选择性地突出具有特定光学厚度的结构。与传统的宽视场显微镜相比,这种设计保留了横向分辨率,同时提供了20倍的对比度增强。人类口腔上皮细胞、血液成分和大肠杆菌的实验显示亚细胞特征和细胞类型分化的清晰可视化。值得注意的是,FPM通过光谱调谐而不是机械扫描实现成像,确保了更快、更稳定的运行。该系统与标准显微镜光学兼容,可在静态和流动条件下工作,为高通量细胞术、生物研究和体外诊断提供了巨大的潜力。这些结果建立了FPM作为宽视场显微镜的多功能扩展,使生物医学样品的对比度可调,定量成像。
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引用次数: 0
Use of High-Power Diode Laser in Neonatal Lingual Frenulum Release Surgery: Clinical Approach and Assessment of Healing in a Case Series 高功率二极管激光在新生儿舌系带释放手术中的应用:临床方法和病例系列的愈合评估。
IF 2 3区 物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS Pub Date : 2026-01-18 DOI: 10.1002/jbio.70218
Adriana Cátia Mazzoni, Amanda Rafaelly Honório Mandetta, Manoela Domingues Martins, Cinthya Cosme Gutierrez Duran, Raquel Agnelli Mesquita-Ferrari, Anna Carolina Ratto Tempestini Horliana, Kristianne Porta Santos Fernandes, Lara Jansiski Motta, Sandra Kalil Bussadori

Ankyloglossia is a congenital condition characterized by restricted tongue mobility due to an abnormal lingual frenum, which may impair breastfeeding in newborns and infants. Early diagnosis and treatment are essential to prevent functional complications. This case series describes 35 newborns and infants with ankyloglossia who underwent frenectomy using a super-pulsed dual-wavelength diode laser. The procedures were performed using a diode laser operating at wavelengths of 810 nm (1.2 W) and 980 nm (1.4 W), delivering a final output power of 1.1 W, in super-pulsed mode (20 Hz frequency, 0.05 ms pulse width), with a 400-μm fiber optic tip. Healing and tongue mobility were evaluated immediately after surgery and at seven and 15 days. Most cases showed balanced tissue repair, absence of fibrosis, and improved tongue mobility, resulting in effective breastfeeding. Unfavorable outcomes were associated with external factors such as pathological healing history and poor adherence to postoperative care.

强直性咬合是一种先天性疾病,其特征是由于舌系带异常导致舌头活动受限,这可能会损害新生儿和婴儿的母乳喂养。早期诊断和治疗对于预防功能性并发症至关重要。本病例系列描述了35例新生儿和婴儿的强直性咬合,他们接受了超脉冲双波长二极管激光系带切除术。该过程使用工作波长为810 nm (1.2 W)和980 nm (1.4 W)的二极管激光器进行,在超脉冲模式(20 Hz频率,0.05 ms脉冲宽度)下,最终输出功率为1.1 W,尖端为400 μm光纤。术后立即、术后7天和术后15天分别评估患者的愈合情况和舌头活动能力。大多数病例显示组织修复平衡,没有纤维化,舌头活动能力改善,导致有效的母乳喂养。不良结果与病理愈合史和术后护理依从性差等外部因素有关。
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引用次数: 0
Multi-Parameter Deep Learning Combined With Fluorescence Lifetime Imaging Microscopy for Non-Invasive and Label-Free Endometrial Cancer Screening 多参数深度学习结合荧光寿命成像显微镜用于无创无标签子宫内膜癌筛查。
IF 2 3区 物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS Pub Date : 2026-01-18 DOI: 10.1002/jbio.70228
Wenjia Zhao, Yonghui Xie, Jiacheng Zhou, Duantao Hou, Yulan Wang, Yuzhe Liu, Yiyan Fei, Jiong Ma, Lan Mi

This study presents a noninvasive and label-free endometrial cancer screening approach combining fluorescence lifetime imaging microscopy (FLIM) with a multi-parameter deep learning algorithm. The autofluorescence signals of NAD(P)H in cervical exfoliated cells of participants (n = 71) were detected by FLIM. Two key metabolic parameters—mean fluorescence lifetime (tm) and protein-bound fraction (a2)—were extracted using FLIM data, which reflect malignancy-associated biochemical changes. Single-parameter models based on individual parameters (tm or a2) and a multi-parameter model integrating both parameters were constructed. The results showed that single-parameter models suffered from sensitivity-specificity imbalance while the multi-parameter model leveraging feature complementarity significantly improved predictive performance—achieving 100% sensitivity and 92% specificity in external testing, with an AUC of 0.92, representing an improvement of 0.17–0.31 over single-parameter models. These findings suggest that combining multi-parameter deep learning strategies with FLIM holds strong potential for risk prediction of endometrial cancer, offering a new approach for noninvasive clinical screening.

本研究提出了一种结合荧光寿命成像显微镜(FLIM)和多参数深度学习算法的无创、无标记子宫内膜癌筛查方法。用FLIM法检测71例受试者宫颈脱落细胞中NAD(P)H的自身荧光信号。两个关键的代谢参数-平均荧光寿命(tm)和蛋白结合分数(a2)-提取使用FLIM数据,反映恶性肿瘤相关的生化变化。分别构建了基于单个参数(tm或a2)的单参数模型和集成这两个参数的多参数模型。结果表明,单参数模型存在敏感性-特异性不平衡,而利用特征互补的多参数模型显著提高了预测性能,在外部测试中达到100%的灵敏度和92%的特异性,AUC为0.92,比单参数模型提高了0.17-0.31。这些发现表明,将多参数深度学习策略与FLIM相结合在子宫内膜癌的风险预测中具有很强的潜力,为无创临床筛查提供了一种新的途径。
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引用次数: 0
Elesclomol Diminishes Redox Imbalance in Peripheral Tissues of Mblac1 Knockout Mice 埃来氯莫尔降低Mblac1基因敲除小鼠外周组织氧化还原失衡。
IF 2 3区 物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS Pub Date : 2026-01-18 DOI: 10.1002/jbio.70224
Mehrnoosh Neghabi, Parisa Nategh, Anna M. Stauffer, Maureen K. Hahn, Randy D. Blakely, Mahsa Ranji

Objective

To determine whether loss of Mblac1, a gene implicated in copper metabolism and Alzheimer's disease, causes systemic mitochondrial redox imbalance and whether treatment with the copper chaperone elesclomol (ES) can restore this balance.

Methods

We employed 3D cryoimaging to quantify redox ratio (RR) in kidneys and livers of Mblac1 knockout (KO) mice and their wildtype (WT) littermates. Mice of both sexes were administered either vehicle control (C) or ES (10 mg/kg, i.p.).

Results

KO tissues exhibited reduced RR, indicating an oxidized metabolic state. ES treatment recovered 77% of the RR deficit in kidneys and 48% in livers, restoring RR to WT levels.

Conclusion

Mblac1 deletion disrupts mitochondrial redox homeostasis in peripheral tissues, while ES partially reverses this imbalance by restoring Cu(I)-dependent metabolism.

Significance

Optical metabolic imaging reveals Mblac1 as a key regulator of systemic redox balance and supports ES as a potential therapy for Cu-linked metabolic dysfunctions.

目的:确定与铜代谢和阿尔茨海默病有关的Mblac1基因的缺失是否会导致全身性线粒体氧化还原失衡,以及铜伴侣埃雷斯克洛莫尔(ES)治疗是否可以恢复这种平衡。方法:采用三维冷冻成像技术定量Mblac1基因敲除(KO)小鼠及其野生型(WT)幼崽肾脏和肝脏的氧化还原比(RR)。雌雄小鼠分别给予对照(C)或ES (10 mg/kg, i.p.)。结果:KO组织表现出降低的RR,表明氧化代谢状态。ES治疗恢复了肾脏77%的RR缺陷和肝脏48%的RR缺陷,使RR恢复到WT水平。结论:Mblac1缺失破坏了外周组织线粒体氧化还原稳态,而ES通过恢复Cu(I)依赖性代谢部分逆转了这种不平衡。意义:光学代谢成像显示Mblac1是系统性氧化还原平衡的关键调节因子,并支持ES作为铜相关代谢功能障碍的潜在治疗方法。
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引用次数: 0
Confocal Raman Microscopy to Study Silicone Breast Implant's Early-Stage Degradation in Reconstructive Surgery 用共聚焦拉曼显微镜研究硅胶乳房假体在乳房再造手术中的早期退化。
IF 2 3区 物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS Pub Date : 2026-01-18 DOI: 10.1002/jbio.202500510
Duong Phu Le, Richard Younes, Frédéric Cuisinier, Frédéric Bodin, Nadia Bahlouli, Simon Chatelin, Hamideh Salehi

The adverse effects of breast implants, especially silicone breast implants, are increasingly being shown. In addition to complications related to surgical technique and individual patient factors, the implant aging is suspected of being one of the main causes. This study employs the Raman technique, a label-free, non-destructive method that provides chemical composition information, to clarify the structure of the membrane and its gel-bleeding prevention function. The results identified two important markers—diphenylsiloxane and CH3 vibration—that can distinguish the degradation state of silicone breast implants before and after implantation. In addition, two Raman bands at 1159 and 1524 cm−1, indicative of gel yellowing, were identified, providing potential insights into the permeation of external substances into the gel through the membrane. The results are consistent with previous observations and highlight the potential of deep learning models to determine degradation time thresholds based on these identified markers.

隆胸植入物,尤其是硅胶隆胸植入物的不良影响越来越明显。除了与手术技术和患者个体因素相关的并发症外,种植体老化被怀疑是主要原因之一。本研究采用拉曼技术,一种提供化学成分信息的无标记、非破坏性方法,来阐明膜的结构及其凝胶出血预防功能。结果发现,二苯基硅氧烷和CH3振动是区分硅胶隆胸植入前后降解状态的重要标志。此外,在1159和1524 cm-1处发现了两条拉曼带,表明凝胶变黄,这为外界物质通过膜渗透到凝胶中提供了潜在的见解。研究结果与之前的观察结果一致,并突出了深度学习模型基于这些已识别的标记确定降解时间阈值的潜力。
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引用次数: 0
Feasibility of Depth-in-Color En Face Optical Coherence Tomography for Colorectal Polyp Classification Using Ensemble Learning and Score-Level Fusion 基于集成学习和分数级融合的深度彩色人脸光学相干断层扫描在结肠直肠息肉分类中的可行性。
IF 2 3区 物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS Pub Date : 2026-01-18 DOI: 10.1002/jbio.202500292
Andrew D. Thrapp, Sean D'Mello, Constantinos Pitris, Christos Photiou, Genevieve Lamphier, Erica Villareyna-Lopez, Anita Chung, Catriona Grant, Hinnerk Schulz-Hildenbrandt, Oscar Caravaca-Mora, Tiffany Miller, Du-Ri Song, Hamed Khalili, Norman S. Nishioka, Guillermo Tearney

In colorectal cancer screening, accurate detection of precursor lesions is challenging due to their ambiguous surface appearance. Depth-sensitive optical coherence tomography (OCT) with deep learning may improve accuracy. OCT imaging was performed on polyps from 300 subjects. Depth was encoded (surface, mid, deep) in color to generate en face OCT projections. En face projections were then annotated. The projections were then used to train an ensemble network based on the malignant potential of polyps. The area under the curve (AUC) for detecting malignant potential of all polyps was 0.90, and for diminutive polyps (≤ 5 mm), it was 0.88. These results demonstrate a high degree of accuracy in classifying malignant potential ex vivo. Should these results hold in vivo, this algorithm would meet the ASGE's PIVI criteria for NPV, supporting clinical use of OCT for either a lower colon ‘diagnose and leave’ strategy and/or ‘resect and discard’ strategy for diminutive polyps.

在结直肠癌筛查中,由于其表面外观模糊,准确检测前驱病变具有挑战性。结合深度学习的深度敏感光学相干层析成像(OCT)可以提高精度。对300名受试者的息肉进行OCT成像。深度用颜色编码(表面、中间、深层)以生成正面OCT投影。然后对面部投影进行注释。然后用这些投影来训练一个基于息肉恶性潜能的集合网络。所有息肉的曲线下面积(AUC)为0.90,小息肉(≤5mm)的AUC为0.88。这些结果表明,在分类恶性潜在的离体高度准确。如果这些结果在体内成立,该算法将满足ASGE对NPV的PIVI标准,支持临床使用OCT进行下结肠“诊断和离开”策略和/或“切除和丢弃”策略治疗小息肉。
{"title":"Feasibility of Depth-in-Color En Face Optical Coherence Tomography for Colorectal Polyp Classification Using Ensemble Learning and Score-Level Fusion","authors":"Andrew D. Thrapp,&nbsp;Sean D'Mello,&nbsp;Constantinos Pitris,&nbsp;Christos Photiou,&nbsp;Genevieve Lamphier,&nbsp;Erica Villareyna-Lopez,&nbsp;Anita Chung,&nbsp;Catriona Grant,&nbsp;Hinnerk Schulz-Hildenbrandt,&nbsp;Oscar Caravaca-Mora,&nbsp;Tiffany Miller,&nbsp;Du-Ri Song,&nbsp;Hamed Khalili,&nbsp;Norman S. Nishioka,&nbsp;Guillermo Tearney","doi":"10.1002/jbio.202500292","DOIUrl":"10.1002/jbio.202500292","url":null,"abstract":"<p>In colorectal cancer screening, accurate detection of precursor lesions is challenging due to their ambiguous surface appearance. Depth-sensitive optical coherence tomography (OCT) with deep learning may improve accuracy. OCT imaging was performed on polyps from 300 subjects. Depth was encoded (surface, mid, deep) in color to generate en face OCT projections. En face projections were then annotated. The projections were then used to train an ensemble network based on the malignant potential of polyps. The area under the curve (AUC) for detecting malignant potential of all polyps was 0.90, and for diminutive polyps (≤ 5 mm), it was 0.88. These results demonstrate a high degree of accuracy in classifying malignant potential ex vivo. Should these results hold in vivo, this algorithm would meet the ASGE's PIVI criteria for NPV, supporting clinical use of OCT for either a lower colon ‘diagnose and leave’ strategy and/or ‘resect and discard’ strategy for diminutive polyps.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12812504/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146000191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biomolecular Fingerprint of Crohn's Disease: A Comparative Raman Spectroscopic Study of Blood and Tissue Samples 克罗恩病的生物分子指纹:血液和组织样本的拉曼光谱比较研究。
IF 2 3区 物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS Pub Date : 2026-01-18 DOI: 10.1002/jbio.70219
M. Daniyal Ghauri, Maria Victoria Fernandez, Giovanni Santacroce, Sumedha Chanda, Irene Zammarchi, Ivan Capobianco, Sanathana Konugolu Venkata Sekar, Subrata Ghosh, Katarzyna Komolibus, Stefan Andersson-Engels, Marietta Iacucci, Rekha Gautam

Crohn's disease (CD) is typically diagnosed through endoscopy and histological analysis of biopsies. Currently, specific noninvasive biomarkers for accurate diagnosis and monitoring of CD remain unavailable. This study investigates Raman spectroscopy (RS) as a noninvasive diagnostic and monitoring tool for CD. Plasma and biopsy samples from 66 participants (55—CD, 11—controls) were analyzed to identify disease-specific molecular alterations. RS revealed significant spectral differences (p < 0.05) in amino acids, proteins, carbohydrates, and carotenoids. Decreased valine and arginine levels were consistent with mucosal damage and inflammation, supported by reduced carbohydrate signals indicating barrier impairment. Carotenoid depletion reflected oxidative stress-related inflammation. Correlations between plasma and tissue spectra suggested that plasma RS can reflect local tissue inflammation. Despite the small and heterogeneous cohort, RS detected biochemical shifts toward healthy profiles following biologic therapy. These findings support RS as a minimally invasive approach for detecting inflammation-associated molecular alterations, holding potential to guide personalized disease management.

克罗恩病(CD)通常通过内窥镜检查和活检的组织学分析来诊断。目前,用于准确诊断和监测乳糜泻的特异性非侵入性生物标志物仍然缺乏。本研究探讨了拉曼光谱(RS)作为CD的无创诊断和监测工具。分析了66名参与者(55名CD, 11名对照)的血浆和活检样本,以确定疾病特异性的分子改变。RS显示了显著的光谱差异(p
{"title":"Biomolecular Fingerprint of Crohn's Disease: A Comparative Raman Spectroscopic Study of Blood and Tissue Samples","authors":"M. Daniyal Ghauri,&nbsp;Maria Victoria Fernandez,&nbsp;Giovanni Santacroce,&nbsp;Sumedha Chanda,&nbsp;Irene Zammarchi,&nbsp;Ivan Capobianco,&nbsp;Sanathana Konugolu Venkata Sekar,&nbsp;Subrata Ghosh,&nbsp;Katarzyna Komolibus,&nbsp;Stefan Andersson-Engels,&nbsp;Marietta Iacucci,&nbsp;Rekha Gautam","doi":"10.1002/jbio.70219","DOIUrl":"10.1002/jbio.70219","url":null,"abstract":"<p>Crohn's disease (CD) is typically diagnosed through endoscopy and histological analysis of biopsies. Currently, specific noninvasive biomarkers for accurate diagnosis and monitoring of CD remain unavailable. This study investigates Raman spectroscopy (RS) as a noninvasive diagnostic and monitoring tool for CD. Plasma and biopsy samples from 66 participants (55—CD, 11—controls) were analyzed to identify disease-specific molecular alterations. RS revealed significant spectral differences (<i>p</i> &lt; 0.05) in amino acids, proteins, carbohydrates, and carotenoids. Decreased valine and arginine levels were consistent with mucosal damage and inflammation, supported by reduced carbohydrate signals indicating barrier impairment. Carotenoid depletion reflected oxidative stress-related inflammation. Correlations between plasma and tissue spectra suggested that plasma RS can reflect local tissue inflammation. Despite the small and heterogeneous cohort, RS detected biochemical shifts toward healthy profiles following biologic therapy. These findings support RS as a minimally invasive approach for detecting inflammation-associated molecular alterations, holding potential to guide personalized disease management.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12812440/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146000267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Photochemical Crosslinking of Eyelid Tissue by Trans-Conjunctival Exposure to UV-A Radiation: Implications for Biological Safety 经结膜暴露于UV-A辐射后眼睑组织的光化学交联:对生物安全的影响。
IF 2 3区 物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS Pub Date : 2026-01-18 DOI: 10.1002/jbio.202500458
Nadja E. Pop, Shuko Suzuki, Robert G. Tripon, Alexandra I. Manta, Kah Meng Lee, Marc Parisot, Andrei G. Cocisiu, Mark H. B. Radford, Damien G. Harkin, Traian V. Chirila

A procedure based on exposure to ultraviolet-A (UV-A) radiation is in current development for treating pathological conditions of the eyelid generated through the laxity of the tissues. By irradiating the eyelid in the presence of a photosensitizer, photochemical crosslinking of tarsal collagen is induced, leading to its mechanical reinforcement, thus preventing further laxity increase. Here, we investigated potential radiation-induced damage to the target tissues. Ex vivo specimens of ovine tarsal plates were irradiated trans-conjunctivally with UV-A radiation (365 nm) in the presence of riboflavin at increasing irradiances. The specimens were processed histologically and analyzed. Except for episodic minor conjunctival epithelial damage, there was no evidence of injury to resident cells, connective tissue, meibomian glands, or muscles. Based on in vitro histological analysis, the study demonstrated negligible tissue damage induced by irradiation with UV-A, thereby suggesting the crosslinking of tarsal collagen as a possible therapeutic approach for treating eyelid laxity.

目前正在开发一种基于暴露于紫外线-A (UV-A)辐射的程序,用于治疗因组织松弛而产生的眼睑病理状况。通过在光敏剂的作用下照射眼睑,可以诱导跗骨胶原蛋白的光化学交联,导致其机械强化,从而防止松弛进一步增加。在这里,我们研究了潜在的辐射引起的靶组织损伤。在核黄素的作用下,用UV-A (365nm)经结膜照射离体羊跗骨板标本,辐照度增加。对标本进行组织学处理和分析。除了偶发性轻微结膜上皮损伤外,没有证据表明存在细胞、结缔组织、睑板腺或肌肉损伤。基于体外组织学分析,该研究显示UV-A照射引起的组织损伤可忽略,从而提示睑窝胶原交联可能是治疗眼睑松弛的一种治疗方法。
{"title":"Photochemical Crosslinking of Eyelid Tissue by Trans-Conjunctival Exposure to UV-A Radiation: Implications for Biological Safety","authors":"Nadja E. Pop,&nbsp;Shuko Suzuki,&nbsp;Robert G. Tripon,&nbsp;Alexandra I. Manta,&nbsp;Kah Meng Lee,&nbsp;Marc Parisot,&nbsp;Andrei G. Cocisiu,&nbsp;Mark H. B. Radford,&nbsp;Damien G. Harkin,&nbsp;Traian V. Chirila","doi":"10.1002/jbio.202500458","DOIUrl":"10.1002/jbio.202500458","url":null,"abstract":"<div>\u0000 \u0000 <p>A procedure based on exposure to ultraviolet-A (UV-A) radiation is in current development for treating pathological conditions of the eyelid generated through the laxity of the tissues. By irradiating the eyelid in the presence of a photosensitizer, photochemical crosslinking of tarsal collagen is induced, leading to its mechanical reinforcement, thus preventing further laxity increase. Here, we investigated potential radiation-induced damage to the target tissues. Ex vivo specimens of ovine tarsal plates were irradiated trans-conjunctivally with UV-A radiation (365 nm) in the presence of riboflavin at increasing irradiances. The specimens were processed histologically and analyzed. Except for episodic minor conjunctival epithelial damage, there was no evidence of injury to resident cells, connective tissue, meibomian glands, or muscles. Based on in vitro histological analysis, the study demonstrated negligible tissue damage induced by irradiation with UV-A, thereby suggesting the crosslinking of tarsal collagen as a possible therapeutic approach for treating eyelid laxity.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146000264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ex Vivo Assessment of Keratinocytic Carcinomas Using a Fast Mid-Infrared Probe 使用快速中红外探针对角化细胞癌的体外评估。
IF 2 3区 物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS Pub Date : 2026-01-15 DOI: 10.1002/jbio.70226
Maria G. Vazquez de Vasquez, Rebecca C. Bradley, Charles L. Hitchcock, Angela S. Casey, Austin Cusick, James V. Coe, Ronald Siegle

A fast mid-infrared spectral probe was used to record infrared (IR) spectra of fresh skin tissue removed from patients with biopsy-proven keratinocytic cancer (KC) during Mohs surgery. A 150-patient library of IR spectral data was obtained to see if KC on the epidermal side (outside) of tissue samples can be differentiated from adjacent nonmalignant tissue and normal skin. A linear support vector machine model minimized overtraining predicting the contributions to decisions at each step in the spectrum. Training with an equal cost for KC and nontumor cases samples demonstrated a KC sensitivity of 93% ± 5% and specificity 82% ± 6% (threefold testing esd) on the full library. Success in this test problem warrants future studies to see if differentiation of melanoma from benign melanocytic as well as other skin lesions is possible on live skin. This study was approved by the Advarra Institutional Review Board, #PRO00053468.

使用快速中红外光谱探针记录Mohs手术中活检证实的角化细胞癌(KC)患者新鲜皮肤组织的红外(IR)光谱。我们获得了150例患者的红外光谱数据库,以观察组织样本表皮侧(外部)的KC是否可以与邻近的非恶性组织和正常皮肤区分开来。线性支持向量机模型最大限度地减少了过度训练,预测了频谱中每一步对决策的贡献。对KC和非肿瘤病例样本进行同等费用的训练,在全文库上KC敏感性为93%±5%,特异性为82%±6%(三倍测试esd)。这个测试问题的成功保证了未来的研究,看看是否有可能在活皮肤上从良性黑色素细胞和其他皮肤病变中分化出黑色素瘤。该研究已获得Advarra机构审查委员会批准,编号为#PRO00053468。
{"title":"Ex Vivo Assessment of Keratinocytic Carcinomas Using a Fast Mid-Infrared Probe","authors":"Maria G. Vazquez de Vasquez,&nbsp;Rebecca C. Bradley,&nbsp;Charles L. Hitchcock,&nbsp;Angela S. Casey,&nbsp;Austin Cusick,&nbsp;James V. Coe,&nbsp;Ronald Siegle","doi":"10.1002/jbio.70226","DOIUrl":"10.1002/jbio.70226","url":null,"abstract":"<p>A fast mid-infrared spectral probe was used to record infrared (IR) spectra of fresh skin tissue removed from patients with biopsy-proven keratinocytic cancer (KC) during Mohs surgery. A 150-patient library of IR spectral data was obtained to see if KC on the epidermal side (outside) of tissue samples can be differentiated from adjacent nonmalignant tissue and normal skin. A linear support vector machine model minimized overtraining predicting the contributions to decisions at each step in the spectrum. Training with an equal cost for KC and nontumor cases samples demonstrated a KC sensitivity of 93% ± 5% and specificity 82% ± 6% (threefold testing esd) on the full library. Success in this test problem warrants future studies to see if differentiation of melanoma from benign melanocytic as well as other skin lesions is possible on live skin. This study was approved by the Advarra Institutional Review Board, #PRO00053468.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"19 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12807626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145992413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of a Multi-Wavelength Photobiomodulation System for Soothing Skincare 多波长舒缓护肤光生物调节系统的研制
IF 2 3区 物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS Pub Date : 2026-01-14 DOI: 10.1002/jbio.70220
Jinyu Chen, Zihao Zheng, Shanduan Zhang, Jianbo Sun

The skin serves not only as a physical barrier but also plays key roles in immune regulation and sensory perception. This study developed a multi-wavelength photobiomodulation system combining 530 nm (green), 590 nm (yellow), and 630 nm (red) light at an irradiance ratio of 1:2:6, leveraging green light's anti-inflammatory properties and red light's barrier-repair efficacy. In both UV- and sodium lauryl sulfate-induced skin damage models, the combined light significantly reduced inflammation, improved barrier function, and restored expression of Claudin-1 and KRT1. Safety evaluations in rabbits confirmed no ocular or dermal irritation at doses of 6–12 J/cm2. These findings demonstrate that the optimized multi-wavelength approach is both safe and effective, providing a strong scientific foundation for its use in soothing functional skincare products.

皮肤不仅是一个物理屏障,而且在免疫调节和感官知觉中起着关键作用。本研究利用绿光的抗炎特性和红光的屏障修复功效,开发了一种由530nm(绿光)、590nm(黄光)和630nm(红光)组成的多波长光生物调节系统,辐照比为1:2:6。在紫外线和十二烷基硫酸钠诱导的皮肤损伤模型中,联合光照均能显著减轻炎症,改善屏障功能,恢复Claudin-1和KRT1的表达。家兔的安全性评估证实,6-12 J/cm2剂量对眼部或皮肤没有刺激。这些结果表明,优化后的多波长方法安全有效,为其在舒缓功能护肤品中的应用提供了强有力的科学依据。
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引用次数: 0
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Journal of Biophotonics
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