Postoperative corneal biomechanical evaluation is of great significance in clinical monitoring and management since corneal transplantation is one of the main methods to improve visual function. In this paper, we propose an OCE system based on a small ultrasound transducer to realize the in vivo detection of postoperative corneal elasticity in different directions. It was first validated and analyzed by different agar, and then the elasticity changes in normal cornea and post-transplant corneal implants and implant beds were further investigated. Compared with normal corneas, the shear wave velocity of the postoperative cornea decreased from 7.42 ± 1.71 m/s to 4.95 ± 0.35 m/s. Meanwhile, the shear wave velocity of the corneal implant bed was lower than that of the implanted sheet. Therefore, this study reports the first biomechanical measurement of corneal grafts based on the OCE technique, which might provide a potential tool for the postoperative evaluation of clinical patients.
{"title":"In Vivo Imaging and Evaluation of Corneal Biomechanics After Corneal Transplantation by Optical Coherence Elastography","authors":"Gang Shi, Yubao Zhang, Sizhu Ai, Yidi Wang, Yingji Li, Xingdao He, Xinhe Zheng","doi":"10.1002/jbio.202400207","DOIUrl":"10.1002/jbio.202400207","url":null,"abstract":"<div>\u0000 \u0000 <p>Postoperative corneal biomechanical evaluation is of great significance in clinical monitoring and management since corneal transplantation is one of the main methods to improve visual function. In this paper, we propose an OCE system based on a small ultrasound transducer to realize the in vivo detection of postoperative corneal elasticity in different directions. It was first validated and analyzed by different agar, and then the elasticity changes in normal cornea and post-transplant corneal implants and implant beds were further investigated. Compared with normal corneas, the shear wave velocity of the postoperative cornea decreased from 7.42 ± 1.71 m/s to 4.95 ± 0.35 m/s. Meanwhile, the shear wave velocity of the corneal implant bed was lower than that of the implanted sheet. Therefore, this study reports the first biomechanical measurement of corneal grafts based on the OCE technique, which might provide a potential tool for the postoperative evaluation of clinical patients.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhao Yizhuo, Ren Yu, Cai Hongxing, Wang Tingting, Li Dongliang, Wang Yu, Liu Jianguo, Li Teng, Hua Yangyang
Regular monitoring of blood oxygenation is important for disease prevention and treatment. Image photoplethysmography (IPPG) technology is a non-contact physiological parameter detection technology, which has been widely used in blood oxygenation detection. However, traditional imaging devices still have issues such as low detection accuracy, narrower receiving spectral range. In this paper, we proposed two improved detection methods based on the dual-wavelength measurement principle, that is, dual-band IPPG signal ratio method and dual-band IPPG signal AC/DC method. To verify the effectiveness of the two methods, we used different heartbeat period IPPG signals as sample data sets, and combined PLS and RF algorithms for model training, thus obtaining the best data processing method. The experimental results showed that the dual-band IPPG signal AC/DC method can effectively reduce the model training time. This method meets the strong demand for non-contact blood oxygen measurement and provides a new measurement idea.
{"title":"A Study of Data Processing Methods for Non-Contact Multispectral Method of Blood Oxygen Saturation","authors":"Zhao Yizhuo, Ren Yu, Cai Hongxing, Wang Tingting, Li Dongliang, Wang Yu, Liu Jianguo, Li Teng, Hua Yangyang","doi":"10.1002/jbio.202400338","DOIUrl":"10.1002/jbio.202400338","url":null,"abstract":"<div>\u0000 \u0000 <p>Regular monitoring of blood oxygenation is important for disease prevention and treatment. Image photoplethysmography (IPPG) technology is a non-contact physiological parameter detection technology, which has been widely used in blood oxygenation detection. However, traditional imaging devices still have issues such as low detection accuracy, narrower receiving spectral range. In this paper, we proposed two improved detection methods based on the dual-wavelength measurement principle, that is, dual-band IPPG signal ratio method and dual-band IPPG signal AC/DC method. To verify the effectiveness of the two methods, we used different heartbeat period IPPG signals as sample data sets, and combined PLS and RF algorithms for model training, thus obtaining the best data processing method. The experimental results showed that the dual-band IPPG signal AC/DC method can effectively reduce the model training time. This method meets the strong demand for non-contact blood oxygen measurement and provides a new measurement idea.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rapid and accurate identification of bacterial species is essential for the effective treatment of infectious diseases and suppression of antibiotic-resistant strains. The unique autofluorescence properties of bacterial cells are exploited for rapid and cost-effective identification that is suitable for point-of-care applications. Fluorescence spectroscopy is combined with machine learning to improve the diagnostic accuracy. Good training data for machine learning can be obtained to achieve the same diagnostic accuracy for bacterial species as when each wavelength is measured in detail over a broad spectral width. Experiments were performed testing 14 bacterial strains. The excitation-emission matrix was analyzed, and Bayesian optimization was used to identify the most effective combinations of wavelengths. The results showed that fluorescence spectra using three specific excitation light regions or excitation spectra using two broad fluorescence detection regions could be used as supervised data to realize diagnostic accuracy comparable to that obtained with more complex instruments.
{"title":"Leveraging Broad-Spectrum Fluorescence Data and Machine Learning for High-Accuracy Bacterial Species Identification","authors":"Daisuke Mito, Shin-ichiro Okihara, Masakazu Kurita, Nami Hatayama, Yusuke Yoshino, Yoshinobu Watanabe, Katsuhiro Ishii","doi":"10.1002/jbio.202400300","DOIUrl":"10.1002/jbio.202400300","url":null,"abstract":"<div>\u0000 \u0000 <p>Rapid and accurate identification of bacterial species is essential for the effective treatment of infectious diseases and suppression of antibiotic-resistant strains. The unique autofluorescence properties of bacterial cells are exploited for rapid and cost-effective identification that is suitable for point-of-care applications. Fluorescence spectroscopy is combined with machine learning to improve the diagnostic accuracy. Good training data for machine learning can be obtained to achieve the same diagnostic accuracy for bacterial species as when each wavelength is measured in detail over a broad spectral width. Experiments were performed testing 14 bacterial strains. The excitation-emission matrix was analyzed, and Bayesian optimization was used to identify the most effective combinations of wavelengths. The results showed that fluorescence spectra using three specific excitation light regions or excitation spectra using two broad fluorescence detection regions could be used as supervised data to realize diagnostic accuracy comparable to that obtained with more complex instruments.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Juliana Sales Rodrigues Costa, Gabriela Silva, Isabela Carvalho Guimarães, Luis Filipe Rocha Silva, Saulo Soares da Silva, João Paulo de Paula Almeida, Cândido Celso Coimbra, Nivaldo Antonio Parizotto, Fernando Gripp, Marco Fabrício Dias-Peixoto, Elizabethe Adriana Esteves, Fabiano Trigueiro Amorim, Cleber Ferraresi, Flavio de Castro Magalhaes
The aim was to investigate the effects of different volumes of strength training (ST) in association with photobiomodulation (PBMt) in mice fed a high-fat diet (HFD) on insulin resistance (IR). Male Swiss albino mice were fed HFD and performed high- or low-volume (one-third) ST (3 days/week), associated with PBMt (660 nm + 850 nm; ~42 J delivered) or not (lights off). ST improved IR, lowered visceral adiposity and circulating cytokines, and increased skeletal muscle hypertrophy and mitochondrial activity. The smaller volume of ST did not interfere with the improvement in IR, mitochondrial activity, or inflammatory profile, but exerted a smaller effect on visceral adiposity and skeletal muscle hypertrophy. Association with PBMt further improved IR, regardless of ST volume, although it did not affect adiposity, mitochondrial activity, and the inflammatory profile. Interestingly, PBMt positively affected quadriceps, but attenuated gluteus maximus hypertrophy. The association with PBMt induced greater improvement than ST alone.
这项研究的目的是调查不同量的力量训练(ST)与光生物调节(PBMt)相结合对高脂饮食(HFD)小鼠胰岛素抵抗(IR)的影响。雄性瑞士白化小鼠以高脂肪饮食为食,进行大运动量或小运动量(三分之一)ST(3 天/周),同时进行或不进行光生物调节(660 纳米 + 850 纳米;~42 焦耳)(关灯)。ST 改善了红外辐射,降低了内脏脂肪和循环细胞因子,并增加了骨骼肌肥大和线粒体活性。较小容量的 ST 不会影响 IR、线粒体活性或炎症状况的改善,但对内脏脂肪和骨骼肌肥大的影响较小。无论 ST 容量如何,与 PBMt 联合使用可进一步改善 IR,尽管它不会影响脂肪含量、线粒体活性和炎症特征。有趣的是,PBMt 对股四头肌有积极影响,但却削弱了臀大肌肥大。与单纯的 ST 相比,与 PBMt 联合使用能带来更大的改善。
{"title":"Photobiomodulation Enhances the Effect of Strength Training on Insulin Resistance Regardless of Exercise Volume in Mice Fed a High-Fat Diet","authors":"Juliana Sales Rodrigues Costa, Gabriela Silva, Isabela Carvalho Guimarães, Luis Filipe Rocha Silva, Saulo Soares da Silva, João Paulo de Paula Almeida, Cândido Celso Coimbra, Nivaldo Antonio Parizotto, Fernando Gripp, Marco Fabrício Dias-Peixoto, Elizabethe Adriana Esteves, Fabiano Trigueiro Amorim, Cleber Ferraresi, Flavio de Castro Magalhaes","doi":"10.1002/jbio.202400274","DOIUrl":"10.1002/jbio.202400274","url":null,"abstract":"<div>\u0000 \u0000 <p>The aim was to investigate the effects of different volumes of strength training (ST) in association with photobiomodulation (PBMt) in mice fed a high-fat diet (HFD) on insulin resistance (IR). Male Swiss albino mice were fed HFD and performed high- or low-volume (one-third) ST (3 days/week), associated with PBMt (660 nm + 850 nm; ~42 J delivered) or not (lights off). ST improved IR, lowered visceral adiposity and circulating cytokines, and increased skeletal muscle hypertrophy and mitochondrial activity. The smaller volume of ST did not interfere with the improvement in IR, mitochondrial activity, or inflammatory profile, but exerted a smaller effect on visceral adiposity and skeletal muscle hypertrophy. Association with PBMt further improved IR, regardless of ST volume, although it did not affect adiposity, mitochondrial activity, and the inflammatory profile. Interestingly, PBMt positively affected quadriceps, but attenuated gluteus maximus hypertrophy. The association with PBMt induced greater improvement than ST alone.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Evaluating the biomechanical properties of the lens and lens capsule is important for the clinical diagnosis and treatment of age-related cataracts and presbyopia. In this study, we developed an optical coherent elastography technique to assess the elasticity of the lens and lens capsule in the human eye. With age, the mean Young's modulus of the lens increased from 12.28 ± 0.87 kPa to 18.59 ± 1.45 kPa, and the lens capsule increased from 6.33 ± 0.36 kPa to 13.33 ± 0.74 kPa. The results showed that the Young's modulus of the lens capsule and lens increased with age, with the Young's modulus of the lens significantly higher than that of the lens capsule. This study reports the assessment of the elasticity of the human lens and lens capsule by the OCE technique, indicating that it may provide a potential clinical tool for advancing research on diseases affecting the lens.
{"title":"Quantitative Evaluation of Human Lens and Lens Capsule Elasticity by Optical Coherence Elastography Based on a Rayleigh Wave Model","authors":"Gang Shi, Yubao Zhang, Yidi Wang, Sizhu Ai, Chaozhong Zhang, Xingdao He, Xinhe Zheng","doi":"10.1002/jbio.202400322","DOIUrl":"10.1002/jbio.202400322","url":null,"abstract":"<div>\u0000 \u0000 <p>Evaluating the biomechanical properties of the lens and lens capsule is important for the clinical diagnosis and treatment of age-related cataracts and presbyopia. In this study, we developed an optical coherent elastography technique to assess the elasticity of the lens and lens capsule in the human eye. With age, the mean Young's modulus of the lens increased from 12.28 ± 0.87 kPa to 18.59 ± 1.45 kPa, and the lens capsule increased from 6.33 ± 0.36 kPa to 13.33 ± 0.74 kPa. The results showed that the Young's modulus of the lens capsule and lens increased with age, with the Young's modulus of the lens significantly higher than that of the lens capsule. This study reports the assessment of the elasticity of the human lens and lens capsule by the OCE technique, indicating that it may provide a potential clinical tool for advancing research on diseases affecting the lens.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lara Maria Bataglia Espósito, Maria Carolina Derencio Oliveira, Luis Henrique Oliveira de Moraes, Camila Pereira Sabadini, Krissia Franco de Godoy, Rafael Afonso Derencio Oliveira, Ana Cláudia Garcia de Oliveira Duarte, Fernanda de Freitas Anibal, Gerson Jhonatan Rodrigues, Patricia Brassolatti, Richard Eloin Liebano
Objective
To evaluate the photobiomodulation effects on the receptor area for full-thickness skin graft integration.
Methods
Thirty-Six Wistar rats were divided: red laser (660 nm), infrared laser (808 nm), and control. A skin segment with 5 × 3 cm was removed. In the control, the skin was reallocated after a 180° rotation. For the 660 nm and 808 nm, the receptor area was first irradiated, and then the skin was reallocated the same as the control. Euthanasia occurred on the third and seventh days after the procedure, and macroscopical of necrosis and histological analysis were realized.
Results
The 660 nm reached the lowest necrosis percentage on Day 7. In the 808 nm, necrosis increased between the two periods. Similar morphological findings were observed for the control and 660 nm; however, the 808 nm showed significant alterations in fibrosis and inflammatory infiltrate.
Conclusion
The infrared wavelength showed inferior performance on skin graft integration compared to the control and the red wavelength.
{"title":"Photobiomodulation on Full-Thickness Skin Graft Survival in Rats","authors":"Lara Maria Bataglia Espósito, Maria Carolina Derencio Oliveira, Luis Henrique Oliveira de Moraes, Camila Pereira Sabadini, Krissia Franco de Godoy, Rafael Afonso Derencio Oliveira, Ana Cláudia Garcia de Oliveira Duarte, Fernanda de Freitas Anibal, Gerson Jhonatan Rodrigues, Patricia Brassolatti, Richard Eloin Liebano","doi":"10.1002/jbio.202400285","DOIUrl":"10.1002/jbio.202400285","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To evaluate the photobiomodulation effects on the receptor area for full-thickness skin graft integration.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Thirty-Six Wistar rats were divided: red laser (660 nm), infrared laser (808 nm), and control. A skin segment with 5 × 3 cm was removed. In the control, the skin was reallocated after a 180° rotation. For the 660 nm and 808 nm, the receptor area was first irradiated, and then the skin was reallocated the same as the control. Euthanasia occurred on the third and seventh days after the procedure, and macroscopical of necrosis and histological analysis were realized.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The 660 nm reached the lowest necrosis percentage on Day 7. In the 808 nm, necrosis increased between the two periods. Similar morphological findings were observed for the control and 660 nm; however, the 808 nm showed significant alterations in fibrosis and inflammatory infiltrate.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The infrared wavelength showed inferior performance on skin graft integration compared to the control and the red wavelength.</p>\u0000 </section>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Silvana Torres Perez, Lucas Andreo, José Antonio Silva Junior, Sandra Kalil Bussadori, Anna Carolina Ratto Tempestini Horliana, Raquel Agnelli Mesquita-Ferrari, Kristianne Porta Santos Fernandes
Photobiomodulation (PBM) has been shown to be promising for the promotion of angiogenesis. The present study investigated the effects of PBM on vascularization in an animal model of peripheral artery disease. Wistar rats were divided into three groups. The control group received no procedures. The ischemia group was submitted to ligation of the femoral artery of the hindleg. The ischemia + PBM group was submitted to ligation of the femoral artery followed by PBM (660 and 808 nm, 100 mW, 4 J) over the site. Animals with ischemia treated with PBM exhibited comparable results to the control group with regards to the diameter of the α-SMA+ vessels, cross-sectional area of muscle fibers, percentage of collagen and serum concentration of IL-17A, as well as similarities in terms of vertical mobility, temperature of the hindleg, number of acts of grooming, and percentage of movement, indicating a condition like that of limbs unaffected by ischemia.
{"title":"Effect of Photobiomodulation on an Experimental Model of Lower Limb Ischemia","authors":"Silvana Torres Perez, Lucas Andreo, José Antonio Silva Junior, Sandra Kalil Bussadori, Anna Carolina Ratto Tempestini Horliana, Raquel Agnelli Mesquita-Ferrari, Kristianne Porta Santos Fernandes","doi":"10.1002/jbio.202400305","DOIUrl":"10.1002/jbio.202400305","url":null,"abstract":"<div>\u0000 \u0000 <p>Photobiomodulation (PBM) has been shown to be promising for the promotion of angiogenesis. The present study investigated the effects of PBM on vascularization in an animal model of peripheral artery disease. Wistar rats were divided into three groups. The control group received no procedures. The ischemia group was submitted to ligation of the femoral artery of the hindleg. The ischemia + PBM group was submitted to ligation of the femoral artery followed by PBM (660 and 808 nm, 100 mW, 4 J) over the site. Animals with ischemia treated with PBM exhibited comparable results to the control group with regards to the diameter of the α-SMA+ vessels, cross-sectional area of muscle fibers, percentage of collagen and serum concentration of IL-17A, as well as similarities in terms of vertical mobility, temperature of the hindleg, number of acts of grooming, and percentage of movement, indicating a condition like that of limbs unaffected by ischemia.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hao Guo, Ashley E. Stueck, Jason B. Doppenberg, Yun Suk Chae, Alexey B. Tikhomirov, Haishan Zeng, Marten A. Engelse, Boris L. Gala-Lopez, Anita Mahadevan-Jansen, Ian P. J. Alwayn, Andrea K. Locke, Kevin C. Hewitt
Background and Aims
Hepatic steatosis (HS), particularly macrovesicular steatosis (MaS), influences transplant outcomes. Accurate assessment of MaS is crucial for graft selection. While traditional assessment methods have limitations, non-invasive spectroscopic techniques like Raman and reflectance spectroscopy offer promise. This study aimed to evaluate the efficacy of a portable ambient light-compatible spectroscopic system in assessing global HS and MaS in human liver specimens.
Methods
A two-stage approach was employed on thawed snap-frozen human liver specimens under ambient room light: biochemical validation involving a comparison of fat content from Raman and reflectance intensities with triglyceride (TG) quantifications and histopathological validation, contrasting Raman-derived fat content with evaluations by an expert pathologist and a “Positive Pixel Count” algorithm. Raman and reflectance intensities were combined to discern significant (≥ 10%) discrepancies in global HS and MaS.
Results
The initial set of 16 specimens showed a positive correlation between Raman and reflectance-derived fat content and TG quantifications. The Raman system effectively differentiated minimum-to-severe global and macrovesicular steatosis in the subsequent 66 specimens. A dual-variable prediction algorithm was developed, effectively classifying significant discrepancies (> 10%) between algorithm-estimated global HS and pathologist-estimated MaS.
Conclusion
Our study established the viability and reliability of a portable spectroscopic system for non-invasive HS and MaS assessment in human liver specimens. The compatibility with ambient light conditions and the ability to address limitations of previous methods marks a significant advancement in this field. By offering promising differentiation between global HS and MaS, our system introduces an innovative approach to real-time and quantitative donor HS assessments. The proposed method holds the promise of refining donor liver assessment during liver recovery and ultimately enhancing transplantation outcomes.
{"title":"Evaluation of Minimum-to-Severe Global and Macrovesicular Steatosis in Human Liver Specimens: A Portable Ambient Light-Compatible Spectroscopic Probe","authors":"Hao Guo, Ashley E. Stueck, Jason B. Doppenberg, Yun Suk Chae, Alexey B. Tikhomirov, Haishan Zeng, Marten A. Engelse, Boris L. Gala-Lopez, Anita Mahadevan-Jansen, Ian P. J. Alwayn, Andrea K. Locke, Kevin C. Hewitt","doi":"10.1002/jbio.202400292","DOIUrl":"10.1002/jbio.202400292","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background and Aims</h3>\u0000 \u0000 <p>Hepatic steatosis (HS), particularly macrovesicular steatosis (MaS), influences transplant outcomes. Accurate assessment of MaS is crucial for graft selection. While traditional assessment methods have limitations, non-invasive spectroscopic techniques like Raman and reflectance spectroscopy offer promise. This study aimed to evaluate the efficacy of a portable ambient light-compatible spectroscopic system in assessing global HS and MaS in human liver specimens.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A two-stage approach was employed on thawed snap-frozen human liver specimens under ambient room light: biochemical validation involving a comparison of fat content from Raman and reflectance intensities with triglyceride (TG) quantifications and histopathological validation, contrasting Raman-derived fat content with evaluations by an expert pathologist and a “Positive Pixel Count” algorithm. Raman and reflectance intensities were combined to discern significant (≥ 10%) discrepancies in global HS and MaS.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The initial set of 16 specimens showed a positive correlation between Raman and reflectance-derived fat content and TG quantifications. The Raman system effectively differentiated minimum-to-severe global and macrovesicular steatosis in the subsequent 66 specimens. A dual-variable prediction algorithm was developed, effectively classifying significant discrepancies (> 10%) between algorithm-estimated global HS and pathologist-estimated MaS.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Our study established the viability and reliability of a portable spectroscopic system for non-invasive HS and MaS assessment in human liver specimens. The compatibility with ambient light conditions and the ability to address limitations of previous methods marks a significant advancement in this field. By offering promising differentiation between global HS and MaS, our system introduces an innovative approach to real-time and quantitative donor HS assessments. The proposed method holds the promise of refining donor liver assessment during liver recovery and ultimately enhancing transplantation outcomes.</p>\u0000 </section>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202400292","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xin Su, Jianhui Wan, Zixi Zheng, Xinyue Xing, Shengde Liu, Shuxian Yang, Liyun Zhong, Xiaoxu Lu
Cerebral microbleeds (CMBs) lead to cognitive decline, linked to the axonal structure composed of phospholipid bilayers. Current methods are difficult to obtain in situ changes of biochemical component concentration during CMB. In this study, by Raman spectrum and two-photon imaging, we achieve in situ changes in the information of biochemical components concentration during CMB. The overall concentration of phospholipids in the damaged tissue significantly decreases after CMB, forming a large region of low concentration, but the relative concentration of phosphatidylinositol (PI) increases, reflecting the inhibition role of the phosphatidylinositol 3 kinase/protein kinase B (PI3K/Akt) pathway. Accordingly, two-photon images of neurons show a clear decrease in the number of axons, indicating a close correlation between phospholipid hydrolysis and axon damage, as well as cognitive impairment. Therefore, the decrease in phospholipid concentration and the increase in the PI concentration might serve as a pair of indicators for characterizing CMB and its relationship with cognitive decline.
脑微出血(CMB)会导致认知能力下降,这与由磷脂双分子层组成的轴突结构有关。目前的方法难以获得 CMB 期间生化成分浓度的原位变化。在本研究中,我们通过拉曼光谱和双光子成像技术实现了 CMB 过程中生化成分浓度信息的原位变化。CMB后,受损组织中磷脂的整体浓度明显降低,形成大片低浓度区域,但磷脂酰肌醇(PI)的相对浓度增加,反映了磷脂酰肌醇3激酶/蛋白激酶B(PI3K/Akt)通路的抑制作用。相应地,神经元的双光子图像显示轴突数量明显减少,表明磷脂水解和轴突损伤以及认知障碍之间存在密切联系。因此,磷脂浓度的降低和 PI 浓度的升高可作为描述 CMB 及其与认知功能衰退关系的一对指标。
{"title":"A Pair of Indicators for Characterizing Cerebral Microbleeds Based on Raman Spectrum and Two-Photon Imaging","authors":"Xin Su, Jianhui Wan, Zixi Zheng, Xinyue Xing, Shengde Liu, Shuxian Yang, Liyun Zhong, Xiaoxu Lu","doi":"10.1002/jbio.202400247","DOIUrl":"10.1002/jbio.202400247","url":null,"abstract":"<div>\u0000 \u0000 <p>Cerebral microbleeds (CMBs) lead to cognitive decline, linked to the axonal structure composed of phospholipid bilayers. Current methods are difficult to obtain in situ changes of biochemical component concentration during CMB. In this study, by Raman spectrum and two-photon imaging, we achieve in situ changes in the information of biochemical components concentration during CMB. The overall concentration of phospholipids in the damaged tissue significantly decreases after CMB, forming a large region of low concentration, but the relative concentration of phosphatidylinositol (PI) increases, reflecting the inhibition role of the phosphatidylinositol 3 kinase/protein kinase B (PI3K/Akt) pathway. Accordingly, two-photon images of neurons show a clear decrease in the number of axons, indicating a close correlation between phospholipid hydrolysis and axon damage, as well as cognitive impairment. Therefore, the decrease in phospholipid concentration and the increase in the PI concentration might serve as a pair of indicators for characterizing CMB and its relationship with cognitive decline.</p>\u0000 </div>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142402503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alexey N. Semenov, Andrei E. Lugovtsov, Petr B. Ermolinskiy, Alexander V. Priezzhev
The blood rheology in vitro in glass or plastic microfluidic chips is different from that in vivo in blood vessels with similar geometry. Absence of vascular endothelium is suggested to cause these discrepancies. This work aims to perform in vitro measurements of blood microrheologic parameters in a slit microfluidic channel covered with endothelial cells (HUVEC). The laser aggregometry was employed to measure the intensity of laser light, backscattered from the blood flow, as a function of shear stress to evaluate the hydrodynamic strength of red blood cells (RBC) aggregates in terms of critical shear stress (CSS). The results demonstrated a decrease in CSS accompanied by an increase in the accuracy of its measurement at similar shear stresses when endothelial cells were present in the channel. The findings hold valuable implications for advanced approaches for endothelization of microfluidic devices, facilitating the study of blood flow dynamics in physiologically more relevant environment.
{"title":"Laser Aggregometry Assessment of Blood Microrheology in a Slit Fluidic Channel Covered With Endothelial Cells","authors":"Alexey N. Semenov, Andrei E. Lugovtsov, Petr B. Ermolinskiy, Alexander V. Priezzhev","doi":"10.1002/jbio.202400379","DOIUrl":"10.1002/jbio.202400379","url":null,"abstract":"<p>The blood rheology in vitro in glass or plastic microfluidic chips is different from that in vivo in blood vessels with similar geometry. Absence of vascular endothelium is suggested to cause these discrepancies. This work aims to perform in vitro measurements of blood microrheologic parameters in a slit microfluidic channel covered with endothelial cells (HUVEC). The laser aggregometry was employed to measure the intensity of laser light, backscattered from the blood flow, as a function of shear stress to evaluate the hydrodynamic strength of red blood cells (RBC) aggregates in terms of critical shear stress (CSS). The results demonstrated a decrease in CSS accompanied by an increase in the accuracy of its measurement at similar shear stresses when endothelial cells were present in the channel. The findings hold valuable implications for advanced approaches for endothelization of microfluidic devices, facilitating the study of blood flow dynamics in physiologically more relevant environment.</p>","PeriodicalId":184,"journal":{"name":"Journal of Biophotonics","volume":"17 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jbio.202400379","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142402505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}