Pub Date : 2019-10-30DOI: 10.22037/AMLS.V1I2.10296
S. Alavian, S. Jazayeri
Nowadays, the presence of HBV DNA in the absence of HBsAg; occult hepatitis B infection; (OBI), is a known clinical entity along with the rapid influx of research being conducted on its clinical relevance. Biologists and clinicians alike have a recent-standing interest in this regards. OBI has been described in several clinical settings. However, the data on its prevalence among immunized and non-immunized healthy general population, in particular, among health care workers (HCWs) is ambigous. This review attemps to explore the significance of OBI in vaccinated groups as a special subject. The prevalence of OBI among general population, vaccinated children/general population and health care workers were: 157 (5.2%), 222 (6.7%) and 33 (1.8%), respectively. The prevalence of anti-HBc among OBI-positive subjects were: 64 (40.7%), 133 (82.7%) and 27 (81.8%), respectively. OBI is partly prevalent in general population and in vaccinated individuals, especially in those who born to HBsAg positive mothers. HBV serological surveys are not enough adequate and sensitive to rule out the presence of HBV DNA. For high-risk groups (subjects born to HBsAg mothers, health care workers, isolated anti-HBc, etc) sensitive molecular tests based on real time PCR should be applied for a proper diagnosis.
{"title":"Occult Hepatitis B infection (OBI) in vaccinated groups, a metanalysis","authors":"S. Alavian, S. Jazayeri","doi":"10.22037/AMLS.V1I2.10296","DOIUrl":"https://doi.org/10.22037/AMLS.V1I2.10296","url":null,"abstract":"Nowadays, the presence of HBV DNA in the absence of HBsAg; occult hepatitis B infection; (OBI), is a known clinical entity along with the rapid influx of research being conducted on its clinical relevance. Biologists and clinicians alike have a recent-standing interest in this regards. OBI has been described in several clinical settings. However, the data on its prevalence among immunized and non-immunized healthy general population, in particular, among health care workers (HCWs) is ambigous. This review attemps to explore the significance of OBI in vaccinated groups as a special subject. The prevalence of OBI among general population, vaccinated children/general population and health care workers were: 157 (5.2%), 222 (6.7%) and 33 (1.8%), respectively. The prevalence of anti-HBc among OBI-positive subjects were: 64 (40.7%), 133 (82.7%) and 27 (81.8%), respectively. OBI is partly prevalent in general population and in vaccinated individuals, especially in those who born to HBsAg positive mothers. HBV serological surveys are not enough adequate and sensitive to rule out the presence of HBV DNA. For high-risk groups (subjects born to HBsAg mothers, health care workers, isolated anti-HBc, etc) sensitive molecular tests based on real time PCR should be applied for a proper diagnosis.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80746275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-14DOI: 10.22037/AMLS.V5I4.32145
Mohammad Panji, Vahideh Behmard, Y. Varghaiyan, Zakieh Sadat Sheikhalishahi, Sajjad Peyvasteh, Effat Seyedhashemi, Mana Zakeri, Farkhonde Pooyanfar, Haleh Barmaki
Background: Neonatal jaundice affects one of two newborns around the World and occurs when a baby has a high level of bilirubin in the blood. In recent decades, the cesarean section has increased, accompanied by higher neonatal jaundice risks compared with vaginal delivery. This study aimed to investigate the relationship between the jaundice severity and the delivery type and gender among newborns referred to a Qamar Bani Hashem Hospital in Khoy city (West Azerbaijan Province, Iran). Materials and Methods: In this cross-sectional study, we investigated randomly 309 newborns with asymptomatic jaundice, who referred to the hospital, from 2014 to 2018. The results of laboratory tests were recorded for all participating infants. Results: 49.19% of newborns with jaundice were born by cesarean section, and 49.19% were born by vaginal delivery. 141 (45.63%) of babies with jaundice were males, and 168 (54.37%) were females. Mean blood sugar (BS) (p=0.52), urea (p=0.48), creatinine(Cr) (p=0.69), Na (p=0.46), K (p=0.69), Ca (p=0.29), TB (p=0.58) and neonatal weight (p=0.14) within days 3 to 10 were not significantly different between vaginal delivery group and cesarean section group (p>0.05), while direct bilirubin showed significant variations (p<0.05). Mean serum direct bilirubin was 0.22±0.07 mg/dL in the normal vaginal delivery group and 0.25±0.09 mg/dL in the cesarean section group (p<0.05). Also, the level of K (P=0.04) was significantly higher in the male group compared to the female group. Conclusion: Based on our study, there is no significant relationship between the severity of jaundice and the type of delivery and gender in newborns.
{"title":"Relationship between Delivery Type and Jaundice Severity among Newborns Referred to Hospital","authors":"Mohammad Panji, Vahideh Behmard, Y. Varghaiyan, Zakieh Sadat Sheikhalishahi, Sajjad Peyvasteh, Effat Seyedhashemi, Mana Zakeri, Farkhonde Pooyanfar, Haleh Barmaki","doi":"10.22037/AMLS.V5I4.32145","DOIUrl":"https://doi.org/10.22037/AMLS.V5I4.32145","url":null,"abstract":"Background: Neonatal jaundice affects one of two newborns around the World and occurs when a baby has a high level of bilirubin in the blood. In recent decades, the cesarean section has increased, accompanied by higher neonatal jaundice risks compared with vaginal delivery. This study aimed to investigate the relationship between the jaundice severity and the delivery type and gender among newborns referred to a Qamar Bani Hashem Hospital in Khoy city (West Azerbaijan Province, Iran). Materials and Methods: In this cross-sectional study, we investigated randomly 309 newborns with asymptomatic jaundice, who referred to the hospital, from 2014 to 2018. The results of laboratory tests were recorded for all participating infants. Results: 49.19% of newborns with jaundice were born by cesarean section, and 49.19% were born by vaginal delivery. 141 (45.63%) of babies with jaundice were males, and 168 (54.37%) were females. Mean blood sugar (BS) (p=0.52), urea (p=0.48), creatinine(Cr) (p=0.69), Na (p=0.46), K (p=0.69), Ca (p=0.29), TB (p=0.58) and neonatal weight (p=0.14) within days 3 to 10 were not significantly different between vaginal delivery group and cesarean section group (p>0.05), while direct bilirubin showed significant variations (p<0.05). Mean serum direct bilirubin was 0.22±0.07 mg/dL in the normal vaginal delivery group and 0.25±0.09 mg/dL in the cesarean section group (p<0.05). Also, the level of K (P=0.04) was significantly higher in the male group compared to the female group. Conclusion: Based on our study, there is no significant relationship between the severity of jaundice and the type of delivery and gender in newborns.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"78 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2019-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84085073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-14DOI: 10.22037/AMLS.V4I2.25212
A. Ghahghaei, Khatoon Heidari nasab, Fariba Mohammadi Tahroodi, S. Rahimi
Background: Formation of amyloid fibrils has been associated with different protein aggregation diseases. Many studies indicate that many proteins can be converted in vitro into amyloid structures. Isolated ҡ-casein (ҡ-CN) spontaneously forms amyloid fibrils under physiological conditions, so it is a convenient model for researching generic aspect of fibril formation. Materials and Methods: In this study the effect of aqueous extract of S. rosmarinus on the amyloid formation of ҡ-CN in the presence and absence of crowding agent, dextran, have been examined using Thioflavin T binding (ThT) assay, fluorescence spectroscopy, and circular dichroism (CD) spectroscopy. Results: ThT binding assay showed that dextran increased the rate of amyloid fibril formation and S. rosmarinus extract retarded the amyloid fibril formation in κ-CN. In the presence of dextran however, the effect of S. rosmarinus extract on the amyloid formation of ҡ-CN was less than in its absence. Fluorescence spectroscopy results also demonstrated that dextran led to unfolding and increased the exposure hydrophobic area in ҡ-CN. S. rosmarinus extract efficiency decreased the exposure of hydrophobic regions in κ-CN, whereas in the presence of dextran this effect of extract was reduced. CD spectroscopy results exhibited that incubation of κ-CN with S. rosmarinus extract prevented a structural transition to a β-sheet. CD spectroscopy results also indicated that by adding dextran to reduced κ-CN β-sheet structures observed, which indicates structural change. S. rosmarinus extract however, prevented transition to β-sheet structural. Conclusion: In conclusion our finding suggests that S. rosmarinus extract prevents amyloid fibril formation in κ-CN, although this effect decreased in the presence of dextran.
{"title":"Investigating the inhibitory effects of Seidlitzia rosmarinus extract on the amyloid fibril formation of ҡ-casein in the presence of dextran","authors":"A. Ghahghaei, Khatoon Heidari nasab, Fariba Mohammadi Tahroodi, S. Rahimi","doi":"10.22037/AMLS.V4I2.25212","DOIUrl":"https://doi.org/10.22037/AMLS.V4I2.25212","url":null,"abstract":"Background: Formation of amyloid fibrils has been associated with different protein aggregation diseases. Many studies indicate that many proteins can be converted in vitro into amyloid structures. Isolated ҡ-casein (ҡ-CN) spontaneously forms amyloid fibrils under physiological conditions, so it is a convenient model for researching generic aspect of fibril formation. Materials and Methods: In this study the effect of aqueous extract of S. rosmarinus on the amyloid formation of ҡ-CN in the presence and absence of crowding agent, dextran, have been examined using Thioflavin T binding (ThT) assay, fluorescence spectroscopy, and circular dichroism (CD) spectroscopy. Results: ThT binding assay showed that dextran increased the rate of amyloid fibril formation and S. rosmarinus extract retarded the amyloid fibril formation in κ-CN. In the presence of dextran however, the effect of S. rosmarinus extract on the amyloid formation of ҡ-CN was less than in its absence. Fluorescence spectroscopy results also demonstrated that dextran led to unfolding and increased the exposure hydrophobic area in ҡ-CN. S. rosmarinus extract efficiency decreased the exposure of hydrophobic regions in κ-CN, whereas in the presence of dextran this effect of extract was reduced. CD spectroscopy results exhibited that incubation of κ-CN with S. rosmarinus extract prevented a structural transition to a β-sheet. CD spectroscopy results also indicated that by adding dextran to reduced κ-CN β-sheet structures observed, which indicates structural change. S. rosmarinus extract however, prevented transition to β-sheet structural. Conclusion: In conclusion our finding suggests that S. rosmarinus extract prevents amyloid fibril formation in κ-CN, although this effect decreased in the presence of dextran.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87461967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Dirofilariasis is a common helminthic parasitic disease in both domestic and wild animals around the world, with canines as the principal reservoir host and mosquitoes as the vector. Human dirofilariasis has been reported from many parts of the world, including Africa, Australia, the Americas, Europe, and Asia. Case Report: A 40 year old woman from Abadan city Southwest of Iran referred to surgeon with a nodule on her right breast. Ultrasound and mammography revealed a nodule diagnosed as parasitic lesion. After resection of the nodule, tissue was placed in 10% formaldehyde and sent to pathology laboratory. In histopathological examination, cross section of a worm surrounded with necrotic tissue, associated with infiltration of Neutrophils, Eosinophils, and foreign body giant cells observed which was morphologically compatible with Dirofilaria repens. Conclusion: Human dirofilariasis should be considered in endemic regions.
{"title":"Human Dirofilaria repens infection of the breast: a case report","authors":"S. Maraghi, Ali Sameri, Yasser Jeddi","doi":"10.22037/AMLS.V1I1.9403","DOIUrl":"https://doi.org/10.22037/AMLS.V1I1.9403","url":null,"abstract":"Background: Dirofilariasis is a common helminthic parasitic disease in both domestic and wild animals around the world, with canines as the principal reservoir host and mosquitoes as the vector. Human dirofilariasis has been reported from many parts of the world, including Africa, Australia, the Americas, Europe, and Asia. Case Report: A 40 year old woman from Abadan city Southwest of Iran referred to surgeon with a nodule on her right breast. Ultrasound and mammography revealed a nodule diagnosed as parasitic lesion. After resection of the nodule, tissue was placed in 10% formaldehyde and sent to pathology laboratory. In histopathological examination, cross section of a worm surrounded with necrotic tissue, associated with infiltration of Neutrophils, Eosinophils, and foreign body giant cells observed which was morphologically compatible with Dirofilaria repens. Conclusion: Human dirofilariasis should be considered in endemic regions.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86279274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-13DOI: 10.22037/AMLS.V4I1.23075
Shahin Aghamiri, Farhad Hajializadeh, A. Jafarpour, Shiva Bayat, Soodeh Namjoo, F. Zaker
Acute lymphoblastic leukemia (ALL) is a hematological malignant disease characterized by an enhanced self-renewal ability of precursor lymphoid cells whose cell division takes more time than their normal counterparts. ALL occurs most between 2 to 5 years of age and during the sixth decade of life. There is a strong relationship between the time ALL occurs in children and the genetic abnormalities which are identified by the rate of leukemic concordance between identical twins. About 90% of ALL cases do not have a clear etiological mechanism. Genetic syndromes, polymorphic variants genes, germline mutations, and some environmental factors are responsible for less than 10% of ALL predisposition but the pathogenesis mechanism of ALL is not identified precisely. Here we review the recent findings and earlier studies about the pathogenesis of acute lymphoblastic leukemia and its incidence. This article also summarizes the identification of predictive factors for ALL and options available to predict disease recurrence.
{"title":"Pathogenesis of Acute Lymphoblastic Leukemia","authors":"Shahin Aghamiri, Farhad Hajializadeh, A. Jafarpour, Shiva Bayat, Soodeh Namjoo, F. Zaker","doi":"10.22037/AMLS.V4I1.23075","DOIUrl":"https://doi.org/10.22037/AMLS.V4I1.23075","url":null,"abstract":"Acute lymphoblastic leukemia (ALL) is a hematological malignant disease characterized by an enhanced self-renewal ability of precursor lymphoid cells whose cell division takes more time than their normal counterparts. ALL occurs most between 2 to 5 years of age and during the sixth decade of life. There is a strong relationship between the time ALL occurs in children and the genetic abnormalities which are identified by the rate of leukemic concordance between identical twins. About 90% of ALL cases do not have a clear etiological mechanism. Genetic syndromes, polymorphic variants genes, germline mutations, and some environmental factors are responsible for less than 10% of ALL predisposition but the pathogenesis mechanism of ALL is not identified precisely. Here we review the recent findings and earlier studies about the pathogenesis of acute lymphoblastic leukemia and its incidence. This article also summarizes the identification of predictive factors for ALL and options available to predict disease recurrence.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79194668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-13DOI: 10.22037/AMLS.V4I1.23078
F. Piri, M. Ebrahimi, K. Amini
Background: Pseudomonas aeruginosa (P. aeruginosa) is one of the most important causes of infection in burns and intensive care units. Extended-spectrum β-lactamases (ESBLs) production in P. aeruginosa is a major factor in the antibiotic resistance and is thought of as a serious threat to the currently available antibiotic armory. The purpose of this study was to determine the prevalence of CTX-M gene in ESBL-producing P. aeruginosa isolates in burn wound samples. Materials and methods: In this cross-sectional survey, a total of 60 clinical isolates of P. aeruginosa were obtained from patients suffering from burn wound infection referred to major hospitals of Tehran, Iran. After verification by biochemical tests and antimicrobial susceptibility testing, CTX-M gene was identified using PCR method. Results: The results of the molecular analysis of CTX-M gene showed that the prevalence of isolates of P. aeruginosa harboring CTX-M gene was 20% (12/60). Conclusion: The results from this study showed high levels of antibiotic resistance and CTX-M gene among P. aeruginosa isolated samples of burn-wound infections which condition may result in the increased the emergence of multidrug-resistant strains and the failure of therapy This study suggests that detailed data on the CTX-M gene frequency can be useful to achieve the best therapy for infections caused by ESBLs producing P. aeruginosa.
{"title":"Molecular investigation of CTX-M gene in Extended Spectrum β Lactamases (ESBLs) producing Pseudomonas aeruginosa isolated from Iranian patients with burn wound infection","authors":"F. Piri, M. Ebrahimi, K. Amini","doi":"10.22037/AMLS.V4I1.23078","DOIUrl":"https://doi.org/10.22037/AMLS.V4I1.23078","url":null,"abstract":"Background: Pseudomonas aeruginosa (P. aeruginosa) is one of the most important causes of infection in burns and intensive care units. Extended-spectrum β-lactamases (ESBLs) production in P. aeruginosa is a major factor in the antibiotic resistance and is thought of as a serious threat to the currently available antibiotic armory. The purpose of this study was to determine the prevalence of CTX-M gene in ESBL-producing P. aeruginosa isolates in burn wound samples. Materials and methods: In this cross-sectional survey, a total of 60 clinical isolates of P. aeruginosa were obtained from patients suffering from burn wound infection referred to major hospitals of Tehran, Iran. After verification by biochemical tests and antimicrobial susceptibility testing, CTX-M gene was identified using PCR method. Results: The results of the molecular analysis of CTX-M gene showed that the prevalence of isolates of P. aeruginosa harboring CTX-M gene was 20% (12/60). Conclusion: The results from this study showed high levels of antibiotic resistance and CTX-M gene among P. aeruginosa isolated samples of burn-wound infections which condition may result in the increased the emergence of multidrug-resistant strains and the failure of therapy This study suggests that detailed data on the CTX-M gene frequency can be useful to achieve the best therapy for infections caused by ESBLs producing P. aeruginosa.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76769174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-13DOI: 10.22037/AMLS.V4I1.23058
N. Afzali, A. Nakhaei, S. J. H. Vashan, M. Torshizi
Background : This experiment was conducted to evaluate the effects of the specific egg yolk immunoglobulin (IgY) on reducing the defects of experimental aflatoxicosis in broilers. Materials and Methods : In a completely randomized design, a total of 128 Ross 308 broiler chicks were used in 4 treatments, 4 replicates and 8 observations (chicks) for 42 days. Treatments were: 1) control; 2) diet containing 1 ppm aflatoxin B1; 3) diet contaminated with 1 ppm aflatoxin B1 + 0.75 % of egg yolk containing IgY; 4) diet contaminated with 1 ppm aflatoxin B1 + 1.5 % of egg yolk containing IgY. Results : The results showed that the use of aflatoxin containing diet significantly increased the feed conversion ratio, serum cholesterol concentration, serum gamma-glutamyl transferase (GGT), and aspartate aminotransferase (AST). Also, experimental aflatoxicosis resulted in reduced feed intake, weight gain, serum total protein and albumin concentrations (p<0.05); as well as the liver histopathologic lesions. IgY containing egg yolk (0.75% and 1.5%) added to the contaminated ration improved feed intake, weight gain and feed conversion ratio compared to treatment 2 (p<0.05). Compared to treatment 2, serum cholesterol concentration decreased while total protein concentration increased in treatments 3 and 4 (p<0.05). Liver tissue was approximately normal with mild effects on hepatocytes and mild cytoplasmic changes in chicks receiving treatments 3 and 4. Conclusion : It can be concluded that specific IgY is effective in reducing the defects of experimental aflatoxicosis as well as improving performance in broilers.
{"title":"To Reduce the Effects of Experimental Aflatoxicosis in Broiler Chicks Using Specific Egg Yolk Immunoglobulin (IgY)","authors":"N. Afzali, A. Nakhaei, S. J. H. Vashan, M. Torshizi","doi":"10.22037/AMLS.V4I1.23058","DOIUrl":"https://doi.org/10.22037/AMLS.V4I1.23058","url":null,"abstract":"Background : This experiment was conducted to evaluate the effects of the specific egg yolk immunoglobulin (IgY) on reducing the defects of experimental aflatoxicosis in broilers. Materials and Methods : In a completely randomized design, a total of 128 Ross 308 broiler chicks were used in 4 treatments, 4 replicates and 8 observations (chicks) for 42 days. Treatments were: 1) control; 2) diet containing 1 ppm aflatoxin B1; 3) diet contaminated with 1 ppm aflatoxin B1 + 0.75 % of egg yolk containing IgY; 4) diet contaminated with 1 ppm aflatoxin B1 + 1.5 % of egg yolk containing IgY. Results : The results showed that the use of aflatoxin containing diet significantly increased the feed conversion ratio, serum cholesterol concentration, serum gamma-glutamyl transferase (GGT), and aspartate aminotransferase (AST). Also, experimental aflatoxicosis resulted in reduced feed intake, weight gain, serum total protein and albumin concentrations (p<0.05); as well as the liver histopathologic lesions. IgY containing egg yolk (0.75% and 1.5%) added to the contaminated ration improved feed intake, weight gain and feed conversion ratio compared to treatment 2 (p<0.05). Compared to treatment 2, serum cholesterol concentration decreased while total protein concentration increased in treatments 3 and 4 (p<0.05). Liver tissue was approximately normal with mild effects on hepatocytes and mild cytoplasmic changes in chicks receiving treatments 3 and 4. Conclusion : It can be concluded that specific IgY is effective in reducing the defects of experimental aflatoxicosis as well as improving performance in broilers.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85239710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-13DOI: 10.22037/AMLS.V4I1.24090
M. Khosravani, M. Dallal, M. Norouzi
Background: Salmonella enterica subsp. enterica serovar Enteritidis is a food-borne pathogenic bacterium that has recently become resistant to most quinolone antibiotics. The MarA efflux pump plays a significant role in the development of ciprofloxacin resistance in S. Enteritidis strains. The aim of this study was comparative evaluation of anti-efflux activity of Artemisia tournefortiana extract and commercial efflux inhibitor, carbonyl cyanide 3-chlorophenylhydrazone (CCCP) on marA efflux pump gene expression in S. Enteritidis clinical strains. M aterials and Methods: In this experimental study, Artemisia tournefortiana extract was prepared using maceration method. Subsequently, MarA efflux pump was detected in 20 clinical strains of S. Enteritidis via cartwheel and PCR methods. Finally, after treatment of strains with subMIC concentration of extract and 20 µg/L and CCCP, their anti-efflux activity against MarA efflux pump was studied using Real Time PCR. R es ults: The results of cartwheel and PCR methods indicated that all of ciprofloxacin resistant strains had MarA efflux pump. Subsequently, after treatment of strains with subMIC concentration of extract and CCCP, results show that both component have the ability to inhibit the MarA efflux pump, significantly. C onclusion: Considering the results of MarA efflux inhibition by A. tournefortiana and CCCP, it seems that this plant can be used as a potential source of drug use as a suppository pump inhibitor instead of CCCP.
背景:肠沙门氏菌亚种。血清型肠炎是一种食源性致病菌，最近对大多数喹诺酮类抗生素产生耐药性。MarA外排泵在肠炎沙门氏菌耐环丙沙星的发展中起重要作用。本研究的目的是比较评价tournefortiana提取物与市售外排抑制剂羰基氰化物3-氯苯基肼(CCCP)对肠链球菌marA外排泵基因表达的抑制作用。材料与方法:本实验采用浸渍法制备金针蒿提取物。随后，采用翻轮法和PCR法在20株肠炎沙门氏菌中检测到MarA外排泵。最后，用亚mic浓度的提取物和20µg/L的CCCP处理菌株后，用Real Time PCR研究其对MarA外排泵的抗外排活性。结果:侧翻法和PCR法结果表明，所有耐药菌株均存在MarA外排泵。随后，用亚mic浓度的提取物和CCCP处理菌株后，结果表明，这两种成分都有显著抑制MarA外排泵的能力。结论:考虑到金针蒿和CCCP对MarA外排的抑制效果，金针蒿可以作为栓剂泵抑制剂替代CCCP，成为潜在的药物来源。
{"title":"marA efflux pump gene expression in Salmonella enteritidis strains treated with Artemisia tournefortiana hydroalcoholic extract and comparison with commercial efflux pump inhibitor, carbonyl cyanide 3-chlorophenylhydrazone (CCCP)","authors":"M. Khosravani, M. Dallal, M. Norouzi","doi":"10.22037/AMLS.V4I1.24090","DOIUrl":"https://doi.org/10.22037/AMLS.V4I1.24090","url":null,"abstract":"Background: Salmonella enterica subsp. enterica serovar Enteritidis is a food-borne pathogenic bacterium that has recently become resistant to most quinolone antibiotics. The MarA efflux pump plays a significant role in the development of ciprofloxacin resistance in S. Enteritidis strains. The aim of this study was comparative evaluation of anti-efflux activity of Artemisia tournefortiana extract and commercial efflux inhibitor, carbonyl cyanide 3-chlorophenylhydrazone (CCCP) on marA efflux pump gene expression in S. Enteritidis clinical strains. M aterials and Methods: In this experimental study, Artemisia tournefortiana extract was prepared using maceration method. Subsequently, MarA efflux pump was detected in 20 clinical strains of S. Enteritidis via cartwheel and PCR methods. Finally, after treatment of strains with subMIC concentration of extract and 20 µg/L and CCCP, their anti-efflux activity against MarA efflux pump was studied using Real Time PCR. R es ults: The results of cartwheel and PCR methods indicated that all of ciprofloxacin resistant strains had MarA efflux pump. Subsequently, after treatment of strains with subMIC concentration of extract and CCCP, results show that both component have the ability to inhibit the MarA efflux pump, significantly. C onclusion: Considering the results of MarA efflux inhibition by A. tournefortiana and CCCP, it seems that this plant can be used as a potential source of drug use as a suppository pump inhibitor instead of CCCP.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87234788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-15DOI: 10.22037/AMLS.V4I2.26275
R. Ebrahimi, Naghmeh Jannat Alipour, S. Emamgholipour
Background: Obesity with a rapid grow in developed and developing countries has a close association with higher disposition to related diseases such as hypertension. Intracellular functions of sodium, potassium, calcium, phosphate, and iron have been an interested subject in obese patients since their dysregulations are linked to a higher risk of hypertension and other metabolic disorders. Materials and Methods: In this study, the circulating levels of sodium, potassium, calcium, phosphate, and iron were determined in the serum of obese patients compared to normal-weight people. Moreover, we examined the correlation of such electrolytes with the well-known indices of obesity such as body mass index (BMI), waist circumference (WC), hip, triglycerides (TG), cholesterol and other characterizations. Results : The mean levels of sodium, potassium, calcium, phosphate, and iron were significantly different (p < 0.05) in obese patients compared to normal-weight subjects. We observed a positive partial correlation between the levels of these electrolytes and obesity indices such as BMI, WC, hip, and cholesterol. Conclusion : Collectively, the present study suggests the positive correlation between obesity and the indices of metabolic disorders such as hypertension and renal failure according to the observed imbalances in the concentration of electrolytes. Moreover, efforts for diet modification may be helpful in the programs aimed at decreasing the burden of obesity and related disorders.
{"title":"The association between intracellular electrolytes and obesity indices","authors":"R. Ebrahimi, Naghmeh Jannat Alipour, S. Emamgholipour","doi":"10.22037/AMLS.V4I2.26275","DOIUrl":"https://doi.org/10.22037/AMLS.V4I2.26275","url":null,"abstract":"Background: Obesity with a rapid grow in developed and developing countries has a close association with higher disposition to related diseases such as hypertension. Intracellular functions of sodium, potassium, calcium, phosphate, and iron have been an interested subject in obese patients since their dysregulations are linked to a higher risk of hypertension and other metabolic disorders. Materials and Methods: In this study, the circulating levels of sodium, potassium, calcium, phosphate, and iron were determined in the serum of obese patients compared to normal-weight people. Moreover, we examined the correlation of such electrolytes with the well-known indices of obesity such as body mass index (BMI), waist circumference (WC), hip, triglycerides (TG), cholesterol and other characterizations. Results : The mean levels of sodium, potassium, calcium, phosphate, and iron were significantly different (p < 0.05) in obese patients compared to normal-weight subjects. We observed a positive partial correlation between the levels of these electrolytes and obesity indices such as BMI, WC, hip, and cholesterol. Conclusion : Collectively, the present study suggests the positive correlation between obesity and the indices of metabolic disorders such as hypertension and renal failure according to the observed imbalances in the concentration of electrolytes. Moreover, efforts for diet modification may be helpful in the programs aimed at decreasing the burden of obesity and related disorders.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77221293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-15DOI: 10.22037/AMLS.V4I1.21919
H. Musavi, Mojdeh Mahdiannasser, A. Shamshirian, S. Azizi
Background : Type 2 diabetes includes a series of disorders characterized by hyperglycemia, which are followed by dysfunction in insulin secretion. In the clinical laboratory, A1C test defines blood glucose levels over a three-month period. The aim of this study is to evaluate the correlation between HbA1c and hyperlipidemia in patients with type 2 diabetes. Materials and Methods : An cross-sectional study was performed on 209 diabetic patients men (n=109) and women (n=100) with a mean age of 57.1 ± 11.1 years. Diagnosis of diabetes was performed according to the WHO criteria. In this study, venous blood (5 ml) was collected from participants after overnight fasting, and HbA1C levels and lipid profiles were determined using enzymatic methods and auto-analyzer device. Risk factor values (TCH/HDL-C) and atherogenic index of plasma (AIP) (logTG/HDL-c), as well as LDL-C/HDL-C ratio, were calculated. Results : HDL-C and TCH / HDL-C were significantly increased in women. On the other hand, LDL-C/HDL-C and HbA1c levels were significantly higher in men. There was no significant difference in the levels of cholesterol, triglyceride, fast blood sugar (FBS), LDL-C, and AIP between women and men. Cholesterol, FBS, LDL-C and LDL-C/HDL-C also showed a significant direct correlation with HbA1c, but no significant correlation was observed between triglyceride, HDL, AIP, and risk ratio with HbA1c. Conclusion : HbA1c may be considered as a biomarker for dyslipidemia screening in patients with type 2 diabetes
{"title":"A Survey on the association of glycosylated hemoglobin A1C and hyperlipidemia in patients with type 2 diabetes","authors":"H. Musavi, Mojdeh Mahdiannasser, A. Shamshirian, S. Azizi","doi":"10.22037/AMLS.V4I1.21919","DOIUrl":"https://doi.org/10.22037/AMLS.V4I1.21919","url":null,"abstract":"Background : Type 2 diabetes includes a series of disorders characterized by hyperglycemia, which are followed by dysfunction in insulin secretion. In the clinical laboratory, A1C test defines blood glucose levels over a three-month period. The aim of this study is to evaluate the correlation between HbA1c and hyperlipidemia in patients with type 2 diabetes. Materials and Methods : An cross-sectional study was performed on 209 diabetic patients men (n=109) and women (n=100) with a mean age of 57.1 ± 11.1 years. Diagnosis of diabetes was performed according to the WHO criteria. In this study, venous blood (5 ml) was collected from participants after overnight fasting, and HbA1C levels and lipid profiles were determined using enzymatic methods and auto-analyzer device. Risk factor values (TCH/HDL-C) and atherogenic index of plasma (AIP) (logTG/HDL-c), as well as LDL-C/HDL-C ratio, were calculated. Results : HDL-C and TCH / HDL-C were significantly increased in women. On the other hand, LDL-C/HDL-C and HbA1c levels were significantly higher in men. There was no significant difference in the levels of cholesterol, triglyceride, fast blood sugar (FBS), LDL-C, and AIP between women and men. Cholesterol, FBS, LDL-C and LDL-C/HDL-C also showed a significant direct correlation with HbA1c, but no significant correlation was observed between triglyceride, HDL, AIP, and risk ratio with HbA1c. Conclusion : HbA1c may be considered as a biomarker for dyslipidemia screening in patients with type 2 diabetes","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"532 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91550454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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