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Estimating carbon leakage from aviation by combining sectoral and general equilibrium models 结合部门模型和一般均衡模型估算航空业的碳泄漏量
IF 1.6 Q2 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-10-03 DOI: 10.1016/j.mex.2024.102975
This article describes a procedure for estimating carbon leakage from policies targeting aviation based on alternative scenarios. The key innovation to ensure greater robustness is that all scenarios are simulated by two different types of models: a sectoral model for aviation and a computable general equilibrium model.
  • The implementation of scenario simulation in both models is explained
  • The calculation of carbon leakage is explained step by step
本文介绍了一种基于替代方案的航空政策碳泄漏估算程序。为确保更高的稳健性,关键的创新之处在于所有情景均由两种不同类型的模型模拟:航空部门模型和可计算一般均衡模型。
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引用次数: 0
A sustainable methodological approach for mitigation of salt stress of rice seedlings in coastal regions: Identification of halotolerant rhizobacteria from Noakhali, Bangladesh and their impact 缓解沿海地区水稻秧苗盐胁迫的可持续方法:孟加拉国诺阿哈利耐盐根瘤菌的鉴定及其影响
IF 1.6 Q2 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-10-01 DOI: 10.1016/j.mex.2024.102981
Salinity hinders the growth of many crops common in the diet, such as rice, wheat and maize when cultivated in coastal salinity areas. Given the limited availability of cultivable land and the increasing growth of the population, it is necessary to enhance productivity. In this paper, we present an innovative approach to adopting Halotolerant Plant Growth Promoting Rhizobacteria (HPGPR) to enhance salt-tolerant rice varieties to solve salinity stress and enhance crop production. HPGPR has functions to overcome plant growth and development and is the most efficient bioinoculant for rice in saline environments. This approach can be considered a potential method because of the cost-effective and environmentally friendly impacts in agricultural production, which involves salt-affected areas.
在沿海盐碱地区种植水稻、小麦和玉米等许多常见作物时,盐碱会阻碍其生长。鉴于可耕地有限和人口日益增长,有必要提高生产力。本文提出了一种创新方法,即采用耐盐植物生长促进根瘤菌(HPGPR)来改良耐盐水稻品种,以解决盐胁迫问题,提高作物产量。HPGPR 具有促进植物生长发育的功能,是盐碱环境中水稻最有效的生物接种剂。这种方法可被视为一种潜在的方法,因为它在农业生产中具有成本效益和环境友好的影响,涉及受盐分影响的地区。
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引用次数: 0
Exploring the change management framework: An in-depth investigation 探索变革管理框架:深入调查
IF 1.6 Q2 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-09-28 DOI: 10.1016/j.mex.2024.102978
Change management is part of Software Development Life Cycle (SDLC), particularly during the challenging stage of Requirement Engineering (RE). Managing requirements and incorporating changes can be complex and demanding, Having high impacts on project duration, cost, and ultimately project success and failure, cost, and ultimately, the success or failure of the software project. This research paper aims to explore and analyses various change management frameworks that contribute to the effectiveness of project completion, with a focus on minimizing time and cost estimates while maximizing product quality. Additionally, this study investigates the success and failure rates of software projects, specifically examining the identification of change management frameworks and addressing critical challenges within the context of Global Software Development (GSD). This review provides valuable insights and recommendations for implementing effective change management practices in software development projects that may facilitate future advancement in change management. This paper reviews various research papers and identifies major problems associated with each existing framework on Time constraints, Quick cost estimates, Verification of used and excluded needs, and customer information. We believe that the finding of this paper helps to address those challenges and reduce overall development time and increase the success rate of projects through the review of Requirement change management identification of a framework.
变更管理是软件开发生命周期(SDLC)的一部分,尤其是在具有挑战性的需求工程(RE)阶段。管理需求和纳入变更可能既复杂又苛刻,对项目持续时间、成本以及最终的项目成败都有很大影响。本研究论文旨在探索和分析有助于提高项目完成效率的各种变更管理框架,重点是最大限度地减少时间和成本估算,同时最大限度地提高产品质量。此外,本研究还调查了软件项目的成功率和失败率,特别研究了在全球软件开发(GSD)背景下确定变更管理框架和应对关键挑战的问题。本综述为在软件开发项目中实施有效的变更管理实践提供了宝贵的见解和建议,可促进变更管理的未来发展。本文回顾了各种研究论文,并指出了与时间限制、快速成本估算、已使用和排除需求的验证以及客户信息等现有框架相关的主要问题。我们相信,本文的发现有助于解决这些挑战,并通过审查需求变更管理框架的确定,缩短整体开发时间,提高项目成功率。
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引用次数: 0
Enhancing protein signal detection in asexual and viviparous pea aphids: A guided protocol for tissue dissection and proteinase K treatment 加强对无性和有性豌豆蚜虫蛋白质信号的检测:组织解剖和蛋白酶 K 处理指导方案
IF 1.6 Q2 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-09-27 DOI: 10.1016/j.mex.2024.102982
Aphids, as hemipteran insects, reproduce via parthenogenesis and viviparity, resulting in rapid and exponential offspring production. To investigate the molecular mechanisms underlying parthenogenetic viviparity in asexual aphids, precise protein detection through immunostaining is essential. Our previous research demonstrated the need for proteinase K (PK) treatment to improve tissue permeability, enabling antibodies targeting the germ-cell marker Ap-Vas1 to access gastrulating and later-stage embryos. However, optimal PK digestion protocols have not been thoroughly explored. In this study, we propose strategies to optimize PK digestion conditions for early, middle, and late-stage pea aphid embryos, which have varying tissue thicknesses. Additionally, we extend the application of PK treatment to salivary glands, a representative somatic tissue, by optimizing conditions for antibody penetration against the salivary gland marker C002. To enhance spatial precision in signal detection, we provide a detailed protocol for tissue dissection specific to pea aphids, focusing on the preservation of tissue integrity. These comprehensive guidelines, covering tissue dissection and PK titration, are expected to improve the specificity and intensity of protein signals in pea aphids and other aphid species.
  • Provide aphid-specific dissection methods to obtain intact embryos and salivary glands.
  • Present strategies for optimizing PK treatment conditions across different tissue types.
蚜虫是一种半翅目昆虫,通过孤雌生殖和胎生繁殖,其后代繁殖迅速且呈指数增长。要研究无性蚜虫孤雌生殖的分子机制,必须通过免疫染色法精确检测蛋白质。我们之前的研究表明,需要用蛋白酶 K(PK)处理来提高组织的通透性,使针对生殖细胞标记物 Ap-Vas1 的抗体能够进入胚胎期和后期胚胎。然而,最佳的 PK 消化方案尚未得到深入探讨。在本研究中,我们提出了针对组织厚度不同的早期、中期和晚期豌豆蚜胚胎优化 PK 消化条件的策略。此外,我们还通过优化唾液腺标记物 C002 的抗体渗透条件,将 PK 处理扩展到唾液腺这一代表性体细胞组织。为了提高信号检测的空间精确度,我们提供了豌豆蚜特有的详细组织解剖方案,重点是保护组织的完整性。这些涵盖组织解剖和 PK 滴定的综合指南有望提高豌豆蚜和其他蚜虫物种蛋白质信号的特异性和强度。
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引用次数: 0
Building a physics-based virtual refrigerated container filled with fruit in ventilated packaging 构建基于物理学的虚拟冷藏集装箱,在通风包装中装满水果
IF 1.6 Q2 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-09-27 DOI: 10.1016/j.mex.2024.102984
We build a validated physics-based model of a refrigerated container filled with fruit in ventilated packaging. This model of a virtual container is the basis for simulations in an accompanying paper on citrus fruit shipped overseas from South Africa to Europe. The model is used to understand better how the cargo cools and when and where food quality is lost in these supply chains. We build a computational fluid dynamics model with a two-phase porous media approach that simulates the airflow in the container and the cooling process of every fruit. This container can be considered aerodynamically to be a slot-ventilated enclosure. We also model the fruit's thermally-driven quality loss. Using a two-phase porous media approach for the ventilated cargo and modeling temperature-driven fruit quality evolution are two steps forward compared to most existing physics-based refrigerated container models. We validate the porous media model implementation. We define and apply actionable metrics for every fruit inside the cargo, such as remaining shelf life upon arrival and seven-eighths cooling time.
  • This model can help reduce food loss and increase supply-chain resilience.
  • This model is an essential building block of a refrigerated container’s digital twin.
  • This model can support stakeholders in improving cargo temperature control and resulting fruit quality preservation.
我们建立了一个经过验证的基于物理的冷藏集装箱模型,其中装满了通风包装的水果。这个虚拟集装箱模型是一篇关于从南非运往欧洲的柑橘类水果海外运输的论文的模拟基础。该模型用于更好地了解货物如何冷却,以及在这些供应链中食品质量损失的时间和地点。我们用两相多孔介质方法建立了一个计算流体动力学模型,模拟集装箱内的气流和每个水果的冷却过程。从空气动力学角度看,这个容器可以被视为一个槽式通风外壳。我们还模拟了水果的热驱动质量损失。与大多数现有的基于物理的冷藏集装箱模型相比,使用两相多孔介质方法来处理通风货物和模拟温度驱动的水果质量演变是向前迈出的两步。我们验证了多孔介质模型的实现。我们为货物中的每种水果定义并应用了可操作的指标,如到达后的剩余保质期和八分之七的冷却时间。
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引用次数: 0
Spray inoculation and image analysis-based quantification of powdery mildew disease severity on pea leaves 基于喷雾接种和图像分析的豌豆叶片白粉病严重程度量化方法
IF 1.6 Q2 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-09-25 DOI: 10.1016/j.mex.2024.102980
Pea (Pisum sativum) is an important agricultural legume crop, but powdery mildew disease caused by the biotrophic fungus Erysiphe pisi regularly limits its annual yield. Assays to evaluate the efficacy of potential antifungal compounds or resistance genes for disease control require a simple fungal inoculation method that provides control over the initial inoculum concentration and enables uniform inoculum distribution within a leaf and across replicates as well as a method for the quantitative assessment of disease severity. Here, we present an easy spray inoculation method for the uniform distribution of a defined concentration of E. pisi conidia on the leaves of pea plants and a semi-automated image analysis-based quantification of disease symptoms. The uniformity in conidial distribution was validated using a novel grading system termed the uniformity index. In addition, RT-qPCR was used to validate the reproducibility of the spray inoculation method and image analysis-based disease quantification. These procedures permit the accurate quantification of powdery mildew disease severity at macroscopic and molecular levels.
  • Uniform and reproducible inoculum distribution on leaves using a simple and inexpensive spray device
  • Rapid and reproducible quantification of powdery mildew disease symptoms using open-source software without the requirement of computational expertise
豌豆(Pisum sativum)是一种重要的农用豆科作物,但由生物营养真菌 Erysiphe pisi 引起的白粉病经常限制其年产量。要评估潜在的抗真菌化合物或抗性基因对病害控制的效果,需要一种简单的真菌接种方法,这种方法能控制初始接种体浓度,使接种体在叶片内和各重复中分布均匀,还需要一种定量评估病害严重程度的方法。在此,我们介绍了一种简便的喷雾接种方法,可在豌豆植株叶片上均匀分布一定浓度的 E. pisi 分生孢子,并可通过半自动图像分析对病害症状进行量化。分生孢子分布的均匀性是通过一种称为均匀性指数的新型分级系统验证的。此外,还利用 RT-qPCR 验证了喷雾接种方法和基于图像分析的病害定量的可重复性。这些程序允许在宏观和分子水平上对白粉病的严重程度进行精确定量。
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引用次数: 0
Combined fluorometric analysis of biliverdin and bilirubin by the recombinant protein HUG 利用重组蛋白 HUG 对胆红素和胆红素进行联合荧光分析
IF 1.6 Q2 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-09-25 DOI: 10.1016/j.mex.2024.102979
Biliverdin is a secondary metabolite of heme catabolism. It is formed by the reaction catalyzed by heme oxygenase, which converts the heme group contained in proteins such as hemoglobin, myoglobin, cytochromes, and catalase into biliverdin, iron (II) and CO in equimolar amounts, consuming NADPH. Biliverdin is then reduced to bilirubin by biliverdin reductase. Biliverdin and bilirubin form a redox couple and are important for the redox homeostasis of cells. Heme oxygenase-1 is an inducible enzyme that is induced by hypoxic conditions, increased availability of heme or proinflammatory mechanisms such as LPS, UV radiation, etc. In addition, both heme oxygenase-1 and biliverdin reductase play roles other than catalysis by modulating specific metabolic pathways at the transcriptional level. There is a need for affordable assays to analyze these bile pigments in biological and clinical samples. Here we present a method for the combined determination of biliverdin and bilirubin that utilizes the specific binding of bilirubin to the fluorescent recombinant fusion protein HUG and the enzymatic conversion of biliverdin to bilirubin.
  • This method enables the combined measurement of bilirubin and biliverdin in the nM range.
  • The method does not require solvent extraction or protein precipitation of the samples.
胆绿素是血红素分解代谢的次级代谢产物。它由血红素加氧酶催化反应生成,该酶将血红蛋白、肌红蛋白、细胞色素和过氧化氢酶等蛋白质中所含的血红素基转化为等摩尔量的胆绿素、铁(II)和 CO,同时消耗 NADPH。然后,胆红素被胆红素还原酶还原成胆红素。胆红素和胆红素形成氧化还原偶联物,对细胞的氧化还原平衡非常重要。血红素加氧酶-1 是一种诱导型酶,缺氧条件、血红素供应量增加或 LPS、紫外线辐射等促炎机制都会诱导这种酶。此外,血红素加氧酶-1 和胆绿素还原酶除了催化作用外,还在转录水平上调节特定的代谢途径。我们需要经济实惠的检测方法来分析生物和临床样本中的这些胆色素。我们在此介绍一种联合测定胆红素和胆红素的方法,该方法利用胆红素与荧光重组融合蛋白 HUG 的特异性结合以及胆红素向胆红素的酶转化。
{"title":"Combined fluorometric analysis of biliverdin and bilirubin by the recombinant protein HUG","authors":"","doi":"10.1016/j.mex.2024.102979","DOIUrl":"10.1016/j.mex.2024.102979","url":null,"abstract":"<div><div>Biliverdin is a secondary metabolite of heme catabolism. It is formed by the reaction catalyzed by heme oxygenase, which converts the heme group contained in proteins such as hemoglobin, myoglobin, cytochromes, and catalase into biliverdin, iron (II) and CO in equimolar amounts, consuming NADPH. Biliverdin is then reduced to bilirubin by biliverdin reductase. Biliverdin and bilirubin form a redox couple and are important for the redox homeostasis of cells. Heme oxygenase-1 is an inducible enzyme that is induced by hypoxic conditions, increased availability of heme or proinflammatory mechanisms such as LPS, UV radiation, etc. In addition, both heme oxygenase-1 and biliverdin reductase play roles other than catalysis by modulating specific metabolic pathways at the transcriptional level. There is a need for affordable assays to analyze these bile pigments in biological and clinical samples. Here we present a method for the combined determination of biliverdin and bilirubin that utilizes the specific binding of bilirubin to the fluorescent recombinant fusion protein HUG and the enzymatic conversion of biliverdin to bilirubin.<ul><li><span>•</span><span><div>This method enables the combined measurement of bilirubin and biliverdin in the nM range.</div></span></li><li><span>•</span><span><div>The method does not require solvent extraction or protein precipitation of the samples.</div></span></li></ul></div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142419744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Psychological disorder detection: A multimodal approach using a transformer-based hybrid model 心理障碍检测:使用基于变压器的混合模型的多模式方法
IF 1.6 Q2 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-09-24 DOI: 10.1016/j.mex.2024.102976
Detecting psychological disorders, particularly depression, is a complex and critical task within the realm of mental health assessment. This research explores a novel approach to improve the identification of psychological distresses, such as depression, by addressing the subjectivity, complexity, and biasness inherent in traditional diagnostic techniques. Using multimodal data, such as voice characteristics and linguistic content from participant interviews, we developed a Transformer-Based Hybrid Model that combines advanced natural language processing and deep learning approaches. This model provides a complete assessment of an individual's psychological well-being by merging aural cues and textual data. This study investigates the theoretical underpinnings, technical complexities, and practical applications of this model in the context of psychological disorder detection. Additionally, the model's design and implementation details are thoroughly documented to ensure replicability by other researchers.
  • A unique way of strengthening emotional ailments (focusing on depression).
  • Transformer-Based Hybrid Model is proposed using multimodal data from interviews of participants.
  • The model integrates voice characteristics (aural cues) and linguistic content (textual data).
  • Comparative analysis of this research with existing approaches.
检测心理障碍,尤其是抑郁症,是心理健康评估领域一项复杂而关键的任务。本研究探索了一种新方法,通过解决传统诊断技术中固有的主观性、复杂性和偏差,改进对抑郁症等心理困扰的识别。我们利用多模态数据,如来自参与者访谈的语音特征和语言内容,开发了一种基于变压器的混合模型,该模型结合了先进的自然语言处理和深度学习方法。该模型通过合并听觉线索和文本数据,提供了对个人心理健康的完整评估。本研究探讨了该模型的理论基础、技术复杂性以及在心理障碍检测中的实际应用。此外,还详细记录了该模型的设计和实施细节,以确保其他研究人员可以复制。-加强情绪疾病(重点是抑郁症)的独特方法-利用参与者访谈中的多模态数据提出基于变压器的混合模型-该模型整合了声音特征(听觉线索)和语言内容(文本数据)-该研究与现有方法的比较分析。
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引用次数: 0
A method to evaluate enhanced rock weathering using intact soil monoliths under field conditions 在野外条件下利用完整土壤单体评估岩石风化强化的方法
IF 1.6 Q2 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-09-23 DOI: 10.1016/j.mex.2024.102971
Enhanced rock weathering (ERW) has attracted considerable attention as a carbon dioxide removal (CDR) strategy. However, a reliable method for accurately measuring, monitoring, and verifying carbon dioxide (CO2) removal, particularly under field conditions, remains elusive. Here we describe a method for installing soil monoliths in an in situ buried apparatus that allows collection of water draining through a soil, undisturbed by external environmental factors that may affect similar apparatus located above ground. The method provides a robust, cost-effective means of collecting, developing, and establishing soil monoliths, allowing through drainage soil water sample collection and analysis, and so facilitating estimation of ERW CO2 removal. A 200 mm diameter polyvinyl chloride (PVC) pipe is inserted into the soil to extract intact monoliths from a site of interest, withdrawn and then fitted with a basal double socket coupling and end cap for leachate collection. It is buried to reproduce soil environmental conditions, and water is collected via a sampling tube to surface. Validity was confirmed through an experimental trial with 36 monoliths over 6 months. This method enables accurate chemical analysis of solute draining through the soil monolith, which can be used to validate models of ERW efficacy.
  • PVC pipes are inserted into the target soil and subsequently extracted to retrieve intact soil monoliths
  • PVC sockets, equipped with a mesh and a geotextile membrane in the middle to retain the collected intact soil monolith and prevent soil particle transport, are then attached to the PVC pipe
  • PVC caps, featuring a small drainage tube attached to its outer side, are used to collect the leachate at the bottom part of the system.
强化岩石风化(ERW)作为一种二氧化碳去除(CDR)策略,已经引起了广泛关注。然而,准确测量、监测和验证二氧化碳(CO2)去除情况的可靠方法,尤其是在野外条件下的去除情况的可靠方法,仍然没有找到。在这里,我们介绍了一种在原地埋设装置中安装土壤单体的方法,这种方法可以收集通过土壤排出的水,不受外部环境因素的干扰,而这些因素可能会影响地面上的类似装置。该方法提供了一种稳健、经济高效的收集、开发和建立土壤单体的方法,允许通过排水系统收集和分析土壤水样本,从而有助于估算战争遗留爆炸物的二氧化碳去除率。将直径为 200 毫米的聚氯乙烯(PVC)管插入土壤中,从感兴趣的地点提取完整的单体,然后抽出,再安装一个基底双承插接头和端盖,用于收集沥滤液。将其埋入地下以再现土壤环境条件,并通过采样管将水收集到地表。通过对 36 块单体进行为期 6 个月的实验测试,证实了该方法的有效性。这种方法能够对通过土壤单体排出的溶质进行精确的化学分析,可用于验证战争遗留爆炸物的功效模型。将聚氯乙烯管插入目标土壤,然后抽出,以回收完整的土壤单体--然后将聚氯乙烯插座连接到聚氯乙烯管上,插座中间装有网眼和土工织物膜,以保留收集到的完整土壤单体并防止土壤颗粒迁移--聚氯乙烯管帽用于收集系统底部的沥滤液,管帽外侧装有小型排水管。
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引用次数: 0
Development of national post-fire restoration system to assess net GHG impacts and salvage biomass availability 开发国家火灾后恢复系统,以评估温室气体净影响和挽救生物量可用性
IF 1.6 Q2 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-09-21 DOI: 10.1016/j.mex.2024.102932
In light of the recent unprecedented wildfires in Canada and the potential for increasing burned areas in the future, there is a need to explore post-fire salvage harvest and restoration and the implications for greenhouse gas (GHG) emissions. Salvage logging and replanting initiatives offer a potential solution by regrowing forests more quickly while meeting societal demands for wood and bioenergy.
This study presents a comprehensive modeling framework to estimate post-fire salvage biomass and net GHG emissions relative to a ‘do-nothing’ baseline for all of Canada's harvest-eligible forests. Forest ecosystem carbon emissions and removals were modeled at 1-ha spatial resolution for Canadian forests using the Generic Carbon Budget Model (GCBM) from 1990 to 2070 using several forest inventory data sources with future harvest and wildfires.
Building upon previous research, our work integrated the Canadian Forest Fire Danger Rating System fire intensity to estimate fire severity of future wildfires. For 2024 to 2070, we assessed the changes in ecosystem carbon, emissions from harvested wood products, and substitution benefits from avoided emissions-intensive materials, relative to a forward-looking baseline. Our prototype system provides a comprehensive framework, configuration files, links to datasets to quantify the net GHG of post-fire restoration, and sample results for validation.
  • Developed spatially explicit forest carbon modeling system for all of Canada's forests.
  • Assessed the net GHG reduction from post-fire restoration.
  • Used system approach to consider forests, wood products and substitution benefits.
鉴于加拿大最近发生了前所未有的野火,而且未来烧毁面积可能会增加,因此有必要探讨火灾后的抢救性采伐和恢复以及对温室气体(GHG)排放的影响。本研究提出了一个全面的建模框架,用于估算加拿大所有符合采伐条件的森林的火灾后挽救性生物量和相对于 "无 "基线的温室气体净排放量。利用通用碳预算模型 (GCBM),以 1 公顷的空间分辨率对加拿大 1990 年至 2070 年的森林生态系统碳排放和碳清除进行了建模,其中使用了若干森林资源清查数据源以及未来的采伐和野火。与前瞻性基线相比,我们评估了 2024 年至 2070 年生态系统碳排放量、伐木产品排放量和避免排放密集型材料的替代效益的变化。我们的原型系统提供了一个综合框架、配置文件、数据集链接,用于量化火灾后恢复的温室气体净排放量,并提供样本结果供验证。
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引用次数: 0
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