MethodsX最新文献_第4页

Efficient data replication in distributed clouds via quantum entanglement algorithms 通过量子纠缠算法在分布式云中进行有效的数据复制

IF 1.9 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX

Pub Date : 2025-12-11 DOI: 10.1016/j.mex.2025.103762

Prabhu Shankar B , RajKumar N , Jayavadivel Ravi , Viji C , Gobinath J , Govindharaj I , Dinesh Kumar K , Elango Muthusamy

In cloud computing, it remains difficult to make data available in a cloud service such that the data is replicated and maintained consistently across various data centers. Traditional replication systems are sufficient, even though they take too long to process, cause significant data transfers, and face problems with final data consistency. This work presents a new method named Quantum Entanglement-Based Replication Algorithm (QERA), which makes use of quantum entanglement to ensure quick and high-performance synchronization of cloud data across all nodes. In this proposed work, the QERA approach encodes data changes in the primary cloud node onto quantum states and entangled qubit pairs to the related replica nodes. As a result, any change is quickly shown on all replicas without the usual overhead and delay of message broadcasts. It simulates how QERA is designed to decrease latency, promote consistency, and make better use of resources in cloud environments. This paper creates a theoretical framework using IBM Qiskit and Microsoft Quantum Development Kit simulators to compare classical and quantum baseline algorithms. The results show that QERA may greatly enhance the way updates and replications are managed across many cloud systems.

It demonstrates how QERA can ensure a very synchronized replication among the remote cloud nodes.

Employs a qubit pair entangled to minimize latency and decrease bandwidth expenses as it goes through updates.

Combines the idea of quantum teleportation with methods of non-invasive verification made to maintain the integrity of the state without altering the quantum system.

在云计算中，仍然很难在云服务中提供数据，以便在各个数据中心之间一致地复制和维护数据。传统的复制系统就足够了，尽管它们需要很长时间来处理，导致大量的数据传输，并且面临最终数据一致性的问题。本文提出了一种基于量子纠缠的复制算法（QERA），该算法利用量子纠缠来保证云数据在所有节点之间的快速、高性能同步。在这项工作中，QERA方法将主云节点中的数据变化编码为量子态，并将纠缠的量子比特对编码到相关的复制节点。因此，任何更改都会迅速显示在所有副本上，而没有通常的开销和消息广播的延迟。它模拟了如何设计QERA来减少延迟、提高一致性和更好地利用云环境中的资源。本文创建了一个理论框架，使用IBM Qiskit和微软量子开发工具包模拟器来比较经典和量子基线算法。结果表明，QERA可以大大增强跨许多云系统管理更新和复制的方式。它演示了QERA如何确保远程云节点之间的复制非常同步。采用纠缠的量子位对，以最大限度地减少延迟并减少更新时的带宽费用。结合了量子隐形传态的思想和非侵入性验证的方法，在不改变量子系统的情况下保持状态的完整性。

{"title":"Efficient data replication in distributed clouds via quantum entanglement algorithms","authors":"Prabhu Shankar B , RajKumar N , Jayavadivel Ravi , Viji C , Gobinath J , Govindharaj I , Dinesh Kumar K , Elango Muthusamy","doi":"10.1016/j.mex.2025.103762","DOIUrl":"10.1016/j.mex.2025.103762","url":null,"abstract":"<div><div>In cloud computing, it remains difficult to make data available in a cloud service such that the data is replicated and maintained consistently across various data centers. Traditional replication systems are sufficient, even though they take too long to process, cause significant data transfers, and face problems with final data consistency. This work presents a new method named Quantum Entanglement-Based Replication Algorithm (QERA), which makes use of quantum entanglement to ensure quick and high-performance synchronization of cloud data across all nodes. In this proposed work, the QERA approach encodes data changes in the primary cloud node onto quantum states and entangled qubit pairs to the related replica nodes. As a result, any change is quickly shown on all replicas without the usual overhead and delay of message broadcasts. It simulates how QERA is designed to decrease latency, promote consistency, and make better use of resources in cloud environments. This paper creates a theoretical framework using IBM Qiskit and Microsoft Quantum Development Kit simulators to compare classical and quantum baseline algorithms. The results show that QERA may greatly enhance the way updates and replications are managed across many cloud systems.</div><div>It demonstrates how QERA can ensure a very synchronized replication among the remote cloud nodes.</div><div>Employs a qubit pair entangled to minimize latency and decrease bandwidth expenses as it goes through updates.</div><div>Combines the idea of quantum teleportation with methods of non-invasive verification made to maintain the integrity of the state without altering the quantum system.</div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103762"},"PeriodicalIF":1.9,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145798570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AI-powered mental health application with data privacy preservation 具有数据隐私保护功能的人工智能心理健康应用程序

IF 1.9 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX

Pub Date : 2025-12-11 DOI: 10.1016/j.mex.2025.103756

Pooja Bagane , Anushree Dahiya , Shardul Kacheria , Ansh Sehgal , Shrishti Bajpai , Obsa Amenu Jebessa

Anxiety disorders, emotional conditions, and stress are becoming an increasing tendency in modern society, which makes digital mental health technologies that provide support in these cases highly demanded. This paper focuses on proposing an AI-driven framework that combines Natural Language Processing (NLP) with privacy-protective systems to recognize emotions in the text of the user. The AES-256 encryption, Supabase authentication, and role-based access control are used to secure data security. It is presented as a multi-label problem of emotion classification, which is fine-tuned on the BERT-base-uncased and ModernBERT Large transformer. ModernBERT exhibited rapid convergence and enhanced situational sensitivity especially when it comes to sarcasm and mixed emotional states.

Key contributions include:

•
Planning an emotion feedback loop offering contextual response and customized mindfulness teaching.
•
Performing a Class imbalance analysis of emotions and trade-offs in precision and recall.
•
Illustrating privacy-first architecture that is able to integrate with digital-therapy and clinician assisted decision software.

在现代社会中，焦虑症、情绪状况和压力正成为一种日益增长的趋势，这使得在这些情况下提供支持的数字心理健康技术受到高度要求。本文的重点是提出一个人工智能驱动的框架，该框架将自然语言处理（NLP）与隐私保护系统相结合，以识别用户文本中的情绪。采用AES-256加密、Supabase认证和基于角色的访问控制，保证数据安全。它是一个多标签的情感分类问题，在bert -base-uncase和ModernBERT Large变压器上进行微调。现代伯特表现出快速收敛和增强的情境敏感性，特别是在涉及讽刺和混合情绪状态时。主要贡献包括：•规划一个情感反馈循环，提供情境反应和定制的正念教学。•执行类的不平衡分析的情绪和权衡的精度和召回。•说明了能够与数字治疗和临床医生辅助决策软件集成的隐私优先架构。

{"title":"AI-powered mental health application with data privacy preservation","authors":"Pooja Bagane , Anushree Dahiya , Shardul Kacheria , Ansh Sehgal , Shrishti Bajpai , Obsa Amenu Jebessa","doi":"10.1016/j.mex.2025.103756","DOIUrl":"10.1016/j.mex.2025.103756","url":null,"abstract":"<div><div>Anxiety disorders, emotional conditions, and stress are becoming an increasing tendency in modern society, which makes digital mental health technologies that provide support in these cases highly demanded. This paper focuses on proposing an AI-driven framework that combines Natural Language Processing (NLP) with privacy-protective systems to recognize emotions in the text of the user. The AES-256 encryption, Supabase authentication, and role-based access control are used to secure data security. It is presented as a multi-label problem of emotion classification, which is fine-tuned on the BERT-base-uncased and ModernBERT Large transformer. ModernBERT exhibited rapid convergence and enhanced situational sensitivity especially when it comes to sarcasm and mixed emotional states.</div><div>Key contributions include:<ul><li><span>•</span><span><div>Planning an emotion feedback loop offering contextual response and customized mindfulness teaching.</div></span></li><li><span>•</span><span><div>Performing a Class imbalance analysis of emotions and trade-offs in precision and recall.</div></span></li><li><span>•</span><span><div>Illustrating privacy-first architecture that is able to integrate with digital-therapy and clinician assisted decision software.</div></span></li></ul></div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103756"},"PeriodicalIF":1.9,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145798169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bifurcation analysis and phase portraits for chiral solitons with bohm potential in quantum hall effect 量子霍尔效应中具有玻姆势的手性孤子的分岔分析和相刻画

IF 1.9 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX

Pub Date : 2025-12-11 DOI: 10.1016/j.mex.2025.103761

Lu Tang , Yakup Yildirim , Ahmed H. Arnous , Ahmed Shaker Mahmood , Ibrahim Zeghaiton Chaloob , Anjan Biswas

This paper presents a comprehensive bifurcation analysis and phase portrait investigation of chiral solitons governed by the chiral nonlinear Schrödinger equation with Bohm potential in the Quantum Hall Effect framework. The equation accounts for chirality, quantum corrections, and nonlinear interactions, making it a valuable model for soliton behavior in quantum fluids. We analyze the system’s dynamical properties, equilibrium points, and solution regimes using bifurcation theory, revealing stability transitions and structural changes in soliton solutions. Phase plane techniques visualize qualitative behaviors under varying parameters. Additionally, we derive exact optical soliton solutions, demonstrating the Bohm potential’s influence on soliton formation and evolution. These findings offer insights into nonlinear wave propagation in chiral quantum systems and have potential applications in condensed matter physics and optical fiber systems.

The paper analyzes chiral solitons using bifurcation theory and phase portraits to reveal stability transitions and dynamic behaviors.

Exact soliton solutions are derived, highlighting the effects of Bohm potential and chirality on soliton formation.

The results provide insights into nonlinear wave propagation in quantum fluids and optical fiber systems.

本文在量子霍尔效应框架下，对具有玻姆势的手性非线性Schrödinger方程控制的手性孤子进行了全面的分岔分析和相画像研究。该方程考虑了手性、量子修正和非线性相互作用，使其成为量子流体中孤子行为的一个有价值的模型。我们利用分岔理论分析了系统的动力学性质、平衡点和解状态，揭示了孤子解的稳定性转变和结构变化。相平面技术可视化在不同参数下的定性行为。此外，我们得到了精确的光学孤子解，证明了玻姆势对孤子形成和演化的影响。这些发现为研究手性量子系统中的非线性波传播提供了新的思路，并在凝聚态物理和光纤系统中具有潜在的应用前景。利用分岔理论和相画像分析手性孤子，揭示其稳定性转变和动力学行为。推导了精确的孤子解，强调了玻姆势和手性对孤子形成的影响。该结果为量子流体和光纤系统中的非线性波传播提供了见解。

{"title":"Bifurcation analysis and phase portraits for chiral solitons with bohm potential in quantum hall effect","authors":"Lu Tang , Yakup Yildirim , Ahmed H. Arnous , Ahmed Shaker Mahmood , Ibrahim Zeghaiton Chaloob , Anjan Biswas","doi":"10.1016/j.mex.2025.103761","DOIUrl":"10.1016/j.mex.2025.103761","url":null,"abstract":"<div><div>This paper presents a comprehensive bifurcation analysis and phase portrait investigation of chiral solitons governed by the chiral nonlinear Schrödinger equation with Bohm potential in the Quantum Hall Effect framework. The equation accounts for chirality, quantum corrections, and nonlinear interactions, making it a valuable model for soliton behavior in quantum fluids. We analyze the system’s dynamical properties, equilibrium points, and solution regimes using bifurcation theory, revealing stability transitions and structural changes in soliton solutions. Phase plane techniques visualize qualitative behaviors under varying parameters. Additionally, we derive exact optical soliton solutions, demonstrating the Bohm potential’s influence on soliton formation and evolution. These findings offer insights into nonlinear wave propagation in chiral quantum systems and have potential applications in condensed matter physics and optical fiber systems.</div><div>The paper analyzes chiral solitons using bifurcation theory and phase portraits to reveal stability transitions and dynamic behaviors.</div><div>Exact soliton solutions are derived, highlighting the effects of Bohm potential and chirality on soliton formation.</div><div>The results provide insights into nonlinear wave propagation in quantum fluids and optical fiber systems.</div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103761"},"PeriodicalIF":1.9,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145798170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reconciled multiplicative relational two-stage network data envelopment analysis 调和乘法关系两阶段网络数据包络分析

IF 1.9 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX

Pub Date : 2025-12-11 DOI: 10.1016/j.mex.2025.103758

M. Burak Erturan

Relational two-stage network DEA (data envelopment analysis) is an approach for two-stage systems, where all input, output and the intermediate products have same weights regardless of which process they are related. One of the main approaches to relational model is the multiplicative relational approach where main process efficiency is the product of two sub-process efficiencies. A shortcoming of the relational model is that the solution of the sub-process efficiencies may not be unique. Researchers have developed some models to overcome this problem, like prioritizing one sub-process to the other or assessing the sub-process efficiencies first and then the main process efficiency. In this study, a novel methodology is presented for a fairer efficiency assessment, where none of the processes is prioritized.

•
Reconciled Multiplicative Relational (RMR) model presented in this study uses data reconciliation method to determine maximum efficiency values for all processes simultaneously within the relational constraints.
•
RMR model has unique efficiency assessment solution, which solves the non-uniqueness problem and useful where none of the DMUs are preferred to another or there is no previous information in that matter.

关系两阶段网络DEA（数据包络分析）是一种针对两阶段系统的方法，其中所有的输入、输出和中间产品无论与哪个过程相关都具有相同的权重。关系模型的主要方法之一是乘法关系方法，其中主流程效率是两个子流程效率的乘积。关系模型的一个缺点是子流程效率的解决方案可能不是唯一的。研究人员已经开发了一些模型来克服这一问题，比如将一个子过程优先于另一个子过程，或者先评估子过程的效率，然后再评估主过程的效率。在这项研究中，提出了一种新的方法来进行更公平的效率评估，其中没有一个过程是优先考虑的。•本研究提出的调和乘法关系（RMR）模型使用数据调和方法在关系约束下同时确定所有流程的最大效率值。•RMR模型具有独特的效率评估方案，解决了非唯一性问题，在没有一个dmu优先于另一个dmu或在该事项中没有先前信息的情况下非常有用。

{"title":"Reconciled multiplicative relational two-stage network data envelopment analysis","authors":"M. Burak Erturan","doi":"10.1016/j.mex.2025.103758","DOIUrl":"10.1016/j.mex.2025.103758","url":null,"abstract":"<div><div>Relational two-stage network DEA (data envelopment analysis) is an approach for two-stage systems, where all input, output and the intermediate products have same weights regardless of which process they are related. One of the main approaches to relational model is the multiplicative relational approach where main process efficiency is the product of two sub-process efficiencies. A shortcoming of the relational model is that the solution of the sub-process efficiencies may not be unique. Researchers have developed some models to overcome this problem, like prioritizing one sub-process to the other or assessing the sub-process efficiencies first and then the main process efficiency. In this study, a novel methodology is presented for a fairer efficiency assessment, where none of the processes is prioritized.<ul><li><span>•</span><span><div>Reconciled Multiplicative Relational (RMR) model presented in this study uses data reconciliation method to determine maximum efficiency values for all processes simultaneously within the relational constraints.</div></span></li><li><span>•</span><span><div>RMR model has unique efficiency assessment solution, which solves the non-uniqueness problem and useful where none of the DMUs are preferred to another or there is no previous information in that matter.</div></span></li></ul></div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103758"},"PeriodicalIF":1.9,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145798162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EMI reduction method of CISPR 36 pre-compliance testing using affordable rubber-based materials CISPR 36预合规测试的EMI减少方法，使用价格合理的橡胶基材料

IF 1.9 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX

Pub Date : 2025-12-10 DOI: 10.1016/j.mex.2025.103760

Arief Rufiyanto , Gamantyo Hendrantoro , Reza Septiawan , Eko Setijadi , Budi Sulistya , Sardjono Trihatmo

Electric motor performance is greatly affected by emissions from the automotive drive system (drivetrain), necessitating research to mitigate electromagnetic interference (EMI). This study proposes a set of methods that employs simple and inexpensive rubber-based materials as shielding to reduce EMI in electric vehicle modules and further explores suitable materials to reduce emissions. The effectiveness of three different rubber compositions as shielding EMI, focusing on the frequency ranges regulated in the CISPR 36 standard, is investigated as pre-compliance testing in radial and transversal orientations of the measurement antenna. The study shows that using these methods together, the rubber-based materials under test can reduce EMI emissions by shielding effectiveness (SE) from 37.742 dB to 37.362 dB for single layer and 74.874 dB to 75.479 dB for combination of 2 layers with up to 50 % probability across several frequency ranges, especially the frequencies regulated in the CISPR 36 standard.

A realistic method to provide a reasonably cost-effective solution to reduce EMI, particularly for electric cars in the pre-compliance stage, using simple and inexpensive materials, mainly rubber-based materials,

EMI mitigation method using organic material as an absorber for pre-compliance testing in the frequency range of the CISPR 36 standard,

Method to determine the best combination of materials to reduce the emissions that arise from the electrical module of the DUT.

电动马达的性能受到汽车驱动系统（传动系统）排放的极大影响，因此需要研究如何减轻电磁干扰（EMI）。本研究提出了一套方法，采用简单和廉价的橡胶基材料作为屏蔽，以减少电动汽车模块中的电磁干扰，并进一步探索合适的材料，以减少排放。在CISPR 36标准规定的频率范围内，研究了三种不同橡胶成分作为屏蔽EMI的有效性，并对测量天线的径向和横向方向进行了预合规测试。研究表明，在多个频率范围内，特别是CISPR 36标准规定的频率范围内，橡胶基材料单层屏蔽效能（SE）从37.742 dB降低到37.362 dB，两层组合屏蔽效能（SE）从74.874 dB降低到75.479 dB，概率高达50%。一种切实可行的方法，提供一种合理的经济有效的解决方案，以减少电磁干扰，特别是在预合规阶段的电动汽车，使用简单和廉价的材料，主要是橡胶基材料；电磁干扰缓解方法，使用有机材料作为吸收剂，在CISPR 36标准的频率范围内进行预合规测试；确定材料的最佳组合，以减少由被测设备的电气模块产生的排放。

{"title":"EMI reduction method of CISPR 36 pre-compliance testing using affordable rubber-based materials","authors":"Arief Rufiyanto , Gamantyo Hendrantoro , Reza Septiawan , Eko Setijadi , Budi Sulistya , Sardjono Trihatmo","doi":"10.1016/j.mex.2025.103760","DOIUrl":"10.1016/j.mex.2025.103760","url":null,"abstract":"<div><div>Electric motor performance is greatly affected by emissions from the automotive drive system (drivetrain), necessitating research to mitigate electromagnetic interference (EMI). This study proposes a set of methods that employs simple and inexpensive rubber-based materials as shielding to reduce EMI in electric vehicle modules and further explores suitable materials to reduce emissions. The effectiveness of three different rubber compositions as shielding EMI, focusing on the frequency ranges regulated in the CISPR 36 standard, is investigated as pre-compliance testing in radial and transversal orientations of the measurement antenna. The study shows that using these methods together, the rubber-based materials under test can reduce EMI emissions by shielding effectiveness (SE) from 37.742 dB to 37.362 dB for single layer and 74.874 dB to 75.479 dB for combination of 2 layers with up to 50 % probability across several frequency ranges, especially the frequencies regulated in the CISPR 36 standard.</div><div>A realistic method to provide a reasonably cost-effective solution to reduce EMI, particularly for electric cars in the pre-compliance stage, using simple and inexpensive materials, mainly rubber-based materials,</div><div>EMI mitigation method using organic material as an absorber for pre-compliance testing in the frequency range of the CISPR 36 standard,</div><div>Method to determine the best combination of materials to reduce the emissions that arise from the electrical module of the DUT.</div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103760"},"PeriodicalIF":1.9,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145798166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of a thermoelectric cooling approach for localized hypothermia in a murine model 热电冷却方法在小鼠模型局部低温治疗中的应用

IF 1.9 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX

Pub Date : 2025-12-09 DOI: 10.1016/j.mex.2025.103754

Kosala D. Waduthanthri , Gregory S. Korbutt , Andrew R. Pepper , Larry D. Unsworth

Achieving localized and adjustable hypothermia is critical for various clinical and experimental applications, including reducing oxidative stress, modulating inflammatory responses, and enabling temperature-triggered drug delivery. However, existing cooling techniques such as ice packs, and cryogenic sprays limitations in precision, efficiency, duration, and cooling capacity. In this study, we used a commercially available thermoelectric cooling module to construct a simple and low-cost cooling system, and applied it in a preclinical mouse model to achieve focal hypothermia at a subcutaneous transplant site.

•
The system, assembled using a TES1–4903 thermoelectric module, a heat sink, and a power supply, achieved rapid temperature reduction rates. At 5 V, the subcutaneous temperature decreased at an average rate of ∼1.5 °C/s during the first 10 s, reaching a stable temperature of ∼8 °C within 120 s. At 2 V, the average rate was ∼0.4 °C/s, stabilizing at ∼17 °C over the same period.
•
The system demonstrated precise temperature control with minimal variability, maintaining temperature steps of <2 °C and ensuring a stable temperature range.
•
Compared to literature, our system highlights the utility of thermoelectric modules for biomedical cooling applications, demonstrating faster and safer subcutaneous hypothermia with more precise temperature control than other approaches.

实现局部和可调节的低温对于各种临床和实验应用至关重要，包括减少氧化应激，调节炎症反应，以及实现温度触发的药物递送。然而，现有的冷却技术，如冰袋和低温喷雾，在精度、效率、持续时间和冷却能力方面存在局限性。在本研究中，我们使用市售的热电冷却模块构建了一个简单、低成本的冷却系统，并将其应用于临床前小鼠模型，实现皮下移植部位的局灶性低温。•该系统使用TES1-4903热电模块、散热器和电源组装，实现了快速降温速率。在5 V下，皮下温度在前10 s内以平均~ 1.5°C/s的速度下降，在120 s内达到稳定的~ 8°C。在2 V时，平均速率为~ 0.4°C/s，在相同的时间内稳定在~ 17°C。•该系统具有精确的温度控制，最小的变化，保持温度步长为<；2°C，并确保稳定的温度范围。•与文献相比，我们的系统突出了热电模块在生物医学冷却应用中的实用性，展示了比其他方法更快，更安全的皮下低温，更精确的温度控制。

{"title":"Application of a thermoelectric cooling approach for localized hypothermia in a murine model","authors":"Kosala D. Waduthanthri , Gregory S. Korbutt , Andrew R. Pepper , Larry D. Unsworth","doi":"10.1016/j.mex.2025.103754","DOIUrl":"10.1016/j.mex.2025.103754","url":null,"abstract":"<div><div>Achieving localized and adjustable hypothermia is critical for various clinical and experimental applications, including reducing oxidative stress, modulating inflammatory responses, and enabling temperature-triggered drug delivery. However, existing cooling techniques such as ice packs, and cryogenic sprays limitations in precision, efficiency, duration, and cooling capacity. In this study, we used a commercially available thermoelectric cooling module to construct a simple and low-cost cooling system, and applied it in a preclinical mouse model to achieve focal hypothermia at a subcutaneous transplant site.<ul><li><span>•</span><span><div>The system, assembled using a TES1–4903 thermoelectric module, a heat sink, and a power supply, achieved rapid temperature reduction rates. At 5 V, the subcutaneous temperature decreased at an average rate of ∼1.5 °C/s during the first 10 s, reaching a stable temperature of ∼8 °C within 120 s. At 2 V, the average rate was ∼0.4 °C/s, stabilizing at ∼17 °C over the same period.</div></span></li><li><span>•</span><span><div>The system demonstrated precise temperature control with minimal variability, maintaining temperature steps of <2 °C and ensuring a stable temperature range.</div></span></li><li><span>•</span><span><div>Compared to literature, our system highlights the utility of thermoelectric modules for biomedical cooling applications, demonstrating faster and safer subcutaneous hypothermia with more precise temperature control than other approaches.</div></span></li></ul></div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103754"},"PeriodicalIF":1.9,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145749538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A method for reconstituting the motility of membrane-bound myosin on the surface of the cell-sized W/O droplet 一种在细胞大小的W/O液滴表面重建膜结合肌球蛋白运动的方法

IF 1.9 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX

Pub Date : 2025-12-09 DOI: 10.1016/j.mex.2025.103755

Yusei Sato , Rieko Sumiyoshi , Masahito Hayashi , Masahiko Yamagishi , Junichiro Yajima

Membrane-bound myosin generates force through interactions with the cytoskeletal actin filament beneath the cell membrane and constitutes the mechanical basis for living cells. Myosin ID, a membrane-bound myosin, drives the gliding motion of actin filaments and binds to phospholipids in the lipid membrane of a living cell. Here, we describe the in-droplet actin filament gliding assay, a method that reconstitutes the motility of actomyosin that dynamically interacts with lipid membranes within water-in-oil (W/O) droplets, which mimic the confined geometry of the intracellular environment. Our method enables quantification of the gliding velocity of actin filaments driven by myosin ID on the inner surface of W/O droplets surrounded by a phospholipid membrane. The in-droplet actin filament gliding assay provides a valuable platform for reconstituting the motile properties of other membrane-bound myosins on membrane surfaces in confined spaces and for analyzing the dynamics of actomyosin networks. The main features and applications of this method are as follows:

•
Reconstitution of actin filament gliding driven by membrane-bound myosin ID within confined water-in-oil droplets.
•
Quantitative evaluation of actomyosin dynamics on the inner surface of the lipid membrane within water-in-oil-droplets.
•
Broadly applicable assay platform for studying the motile properties of membrane-associated myosin families.

膜结合肌球蛋白通过与细胞膜下的细胞骨架肌动蛋白丝相互作用产生力，构成活细胞的机械基础。肌凝蛋白ID是一种膜结合的肌凝蛋白，它驱动肌动蛋白丝的滑动运动，并与活细胞脂质膜中的磷脂结合。在这里，我们描述了液滴内肌动蛋白丝滑动实验，这是一种重建肌动蛋白运动的方法，它与油包水（W/O）液滴内的脂膜动态相互作用，模拟了细胞内环境的受限几何形状。我们的方法可以量化由肌球蛋白ID驱动的肌动蛋白丝在磷脂膜包围的W/O液滴内表面的滑动速度。液滴内肌动蛋白丝滑动试验为重建其他膜结合肌动蛋白在密闭空间膜表面的运动特性和分析肌动蛋白网络的动力学提供了一个有价值的平台。该方法的主要特点和应用如下：•由膜结合肌球蛋白ID驱动的肌动蛋白丝在受限油包水滴内的滑动重构。•定量评价油包水液滴内脂膜表面肌动球蛋白动力学。•广泛适用于研究膜相关肌球蛋白家族运动特性的分析平台。

{"title":"A method for reconstituting the motility of membrane-bound myosin on the surface of the cell-sized W/O droplet","authors":"Yusei Sato , Rieko Sumiyoshi , Masahito Hayashi , Masahiko Yamagishi , Junichiro Yajima","doi":"10.1016/j.mex.2025.103755","DOIUrl":"10.1016/j.mex.2025.103755","url":null,"abstract":"<div><div>Membrane-bound myosin generates force through interactions with the cytoskeletal actin filament beneath the cell membrane and constitutes the mechanical basis for living cells. Myosin ID, a membrane-bound myosin, drives the gliding motion of actin filaments and binds to phospholipids in the lipid membrane of a living cell. Here, we describe the <em>in-droplet</em> actin filament gliding assay, a method that reconstitutes the motility of actomyosin that dynamically interacts with lipid membranes within water-in-oil (W/O) droplets, which mimic the confined geometry of the intracellular environment. Our method enables quantification of the gliding velocity of actin filaments driven by myosin ID on the inner surface of W/O droplets surrounded by a phospholipid membrane. The <em>in-droplet</em> actin filament gliding assay provides a valuable platform for reconstituting the motile properties of other membrane-bound myosins on membrane surfaces in confined spaces and for analyzing the dynamics of actomyosin networks. The main features and applications of this method are as follows:<ul><li><span>•</span><span><div>Reconstitution of actin filament gliding driven by membrane-bound myosin ID within confined water-in-oil droplets.</div></span></li><li><span>•</span><span><div>Quantitative evaluation of actomyosin dynamics on the inner surface of the lipid membrane within water-in-oil-droplets.</div></span></li><li><span>•</span><span><div>Broadly applicable assay platform for studying the motile properties of membrane-associated myosin families.</div></span></li></ul></div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103755"},"PeriodicalIF":1.9,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145798163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An approach to in vivo low-magnification optical coherence tomography for selective monitoring of tissue glucose concentration 一种用于选择性监测组织葡萄糖浓度的体内低倍光学相干断层扫描方法

IF 1.9 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX

Pub Date : 2025-12-08 DOI: 10.1016/j.mex.2025.103752

Akitoshi Seiyama , Fengcheng Wei , Masato Ohmi

The low-magnification optical coherence tomography (LM-OCT) is expected to enable the quantification of tissue glucose concentration (TGC) by maintaining high spatial resolution while reducing artifacts caused by tissue heterogeneity. In the present study, we report the practical application of LM-OCT using a conventional OCT system, i.e., without compromising spatial resolution, for the selective monitoring of changes in TGC through in vivo experiments using a hairless rat model.

A commercially available high-spatial resolution OCT device with a center wavelength of 1300 nm was used to detect the three-dimensional (3D) skin surface structure and the scattering coefficient related to the OCT slope.

The grand-averaged OCT signal was employed to estimate the OCT slope, while the original high-resolution OCT signals were used to reconstruct the 3D-structural image.

A strong linear correlation was observed between changes in the OCT slope and that in TGC, but not with 2-deoxy-glucose, lactate, or Intralipos (soybean oil).

低倍率光学相干断层扫描（LM-OCT）有望通过保持高空间分辨率，同时减少由组织异质性引起的伪影，实现组织葡萄糖浓度（TGC）的量化。在本研究中，我们报告了LM-OCT在传统OCT系统的实际应用，即在不影响空间分辨率的情况下，通过无毛大鼠模型的体内实验，选择性地监测TGC的变化。利用市售的中心波长为1300 nm的高空间分辨率OCT装置检测皮肤的三维（3D）表面结构和与OCT斜率相关的散射系数。利用大平均OCT信号估计OCT斜率，利用原始高分辨率OCT信号重建三维结构图像。OCT斜率的变化与TGC有很强的线性相关性，但与2-脱氧葡萄糖、乳酸或脂肪内酯（大豆油）没有相关性。

引用次数: 0

Application of multiple parallel mineralization method to grow lettuce, Swiss chard, and peppers in a simplified deep flow technique hydroponic system 多重平行矿化法在简化深流技术水培系统中种植生菜、甜菜和辣椒的应用

IF 1.9 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX

Pub Date : 2025-12-06 DOI: 10.1016/j.mex.2025.103749

Jada Tarvin-Imeokparia , Adeyemi Abejoye , Isabella Vega , Max Abel Alphonse Mendoza , Campbell Moses , Myalia Durno , Meghan S. Cahill , Christian O. Dimkpa , Jason C. White , Shelley Durocher , Frederick Pettit , Mark Meyering , Jonathan M. Jacobs , Maria Soledad Benitez Ponce , Nathaniel Heiden

Organic fertilizers are not widely used in hydroponics due to an absence of mineralizing microbes that process organic inputs into plant available forms. The Multiple Parallel Mineralization (MPM) method enables this microbial processing to occur in hydroponic systems. The objective of this method is to provide an accessible protocol for use of the MPM method in simplified deep flow technique hydroponic systems without a need for laboratory testing of nutrient content. The method described here provides hydroponic growers with an alternative option to mineral fertilizers. We provide information about yields and the affordability of this method that growers can use to determine the economic viability of this method in their respective markets.

Swiss chard, lettuce, and bell peppers were grown with this method

Aquarium nitrate test kits are used to replace lab testing typically used with the MPM method to validate the presence of nitrate as an indicator of microbial activity

有机肥料在水培中没有广泛使用，因为没有矿化微生物将有机输入转化为植物可用的形式。多重平行矿化（MPM）方法使这种微生物处理发生在水培系统。该方法的目的是为在简化的深流技术水培系统中使用MPM方法提供一个可访问的协议，而无需实验室测试营养成分。这里描述的方法为水培种植者提供了矿物肥料的另一种选择。我们提供了有关产量和这种方法的可负担性的信息，种植者可以使用这些信息来确定这种方法在各自市场上的经济可行性。瑞士甜菜、生菜和甜椒都是用这种方法种植的。硝酸水族馆测试试剂盒用来取代通常用MPM方法进行的实验室测试，以验证硝酸盐作为微生物活动指标的存在

{"title":"Application of multiple parallel mineralization method to grow lettuce, Swiss chard, and peppers in a simplified deep flow technique hydroponic system","authors":"Jada Tarvin-Imeokparia , Adeyemi Abejoye , Isabella Vega , Max Abel Alphonse Mendoza , Campbell Moses , Myalia Durno , Meghan S. Cahill , Christian O. Dimkpa , Jason C. White , Shelley Durocher , Frederick Pettit , Mark Meyering , Jonathan M. Jacobs , Maria Soledad Benitez Ponce , Nathaniel Heiden","doi":"10.1016/j.mex.2025.103749","DOIUrl":"10.1016/j.mex.2025.103749","url":null,"abstract":"<div><div>Organic fertilizers are not widely used in hydroponics due to an absence of mineralizing microbes that process organic inputs into plant available forms. The Multiple Parallel Mineralization (MPM) method enables this microbial processing to occur in hydroponic systems. The objective of this method is to provide an accessible protocol for use of the MPM method in simplified deep flow technique hydroponic systems without a need for laboratory testing of nutrient content. The method described here provides hydroponic growers with an alternative option to mineral fertilizers. We provide information about yields and the affordability of this method that growers can use to determine the economic viability of this method in their respective markets.</div><div>Swiss chard, lettuce, and bell peppers were grown with this method</div><div>Aquarium nitrate test kits are used to replace lab testing typically used with the MPM method to validate the presence of nitrate as an indicator of microbial activity</div></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":"16 ","pages":"Article 103749"},"PeriodicalIF":1.9,"publicationDate":"2025-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145749534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “From detection to grading: A hybrid KOA-YOLOv5-RF model for knee osteoarthritis diagnosis” [MethodsX, Volume 15, December 2025, 103725] “从检测到分级：一种混合KOA-YOLOv5-RF模型用于膝骨关节炎诊断”的勘误表[MethodsX， vol . 15, December 2025, 103725]

IF 1.9 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX

Pub Date : 2025-12-06 DOI: 10.1016/j.mex.2025.103751

Manikandaprabhu Perumalsamy , Priya Govindarajan , Rinhas Bran , Adarsh Krishna KP , Niranjan V Jyothi , M Batumalay

引用次数: 0