MethodsX最新文献_第3页

Construction and applications of iterative methods for finding approximate solutions of nonlinear equations having unknown zeros of multiplicity with fractal geometry and dynamical behavior 具有分形几何和动力学特性的未知零点非线性方程近似解的迭代方法的构造和应用

IF 1.9 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX

Pub Date : 2026-06-01 Epub Date: 2025-12-19 DOI: 10.1016/j.mex.2025.103778

Farooq Ahmed Shah , Iftikhar Haider , Muhammad Waseem , Alexey Mikhaylov , Nora Baranyai

In this study, several new iterative schemes are developed to compute the multiple zeros of nonlinear equations. The construction of these methods is based on the variational iteration approach which provides a systematic framework for formulating efficient and flexible algorithms. The proposed schemes generalize and encompass well-known classical methods such as Newton’s and Halley’s methods, along with their modified versions as special cases. This generality enhances their adaptability to a broader class of nonlinear problems involving both known and unknown multiplicities. To assess the effectiveness of the proposed iterative schemes, extensive numerical experiments are conducted, comparing their convergence speed and accuracy with existing methods. The results demonstrate that the newly developed methods exhibit superior performance in terms of stability, precision, and computational efficiency. Furthermore, to visualize and analyse the global convergence behaviour fractal basin plots are presented. These fractals illustrate the basins of attraction in the complex plane, providing deeper insight into the dynamical behaviour, convergence regions and boundary structures associated with each iterative process.

•
Developing efficient and flexible iterative methods using the variational iteration method.
•
Generalizing classical methods to tackle nonlinear problems with known and unknown multiplicity.
•
Validating performance of various methods through detailed numerical experiments and fractal basin plots.

在本研究中，提出了几种新的迭代格式来计算非线性方程的多重零。这些方法的构造基于变分迭代法，为制定高效灵活的算法提供了系统框架。所提出的方案概括并包含了著名的经典方法，如牛顿和哈雷的方法，以及作为特殊情况的修改版本。这种普遍性增强了它们对更广泛的非线性问题的适应性，包括已知和未知的多重性。为了评估所提出的迭代方案的有效性，进行了大量的数值实验，比较了它们与现有方法的收敛速度和精度。结果表明，新方法在稳定性、精度和计算效率方面表现出优异的性能。此外，为了可视化和分析全局收敛行为，提出了分形盆地图。这些分形说明了复杂平面上的吸引力盆地，为每个迭代过程相关的动力学行为、收敛区域和边界结构提供了更深入的了解。•使用变分迭代法开发高效灵活的迭代方法。•推广经典方法来解决具有已知和未知多重性的非线性问题。•通过详细的数值实验和分形盆地图验证各种方法的性能。

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引用次数: 0

Knowledge, support, and networking for Phelan-McDermid syndrome: a study protocol 费伦-麦克德米德综合征的知识、支持和网络：一项研究方案

IF 1.9 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX

Pub Date : 2026-06-01 Epub Date: 2025-12-16 DOI: 10.1016/j.mex.2025.103771

Luca Colnaghi , Giulia Villa , Ilaria Marcomini , Andrea Poliani , Maya Fedeli , Claudio Losi , Debora Rosa , Duilio Fiorenzo Manara

Background

Phelan-McDermid syndrome (PMS) is a rare neurodevelopmental disorder caused by deletions in chromosome 22q13.3 or pathogenic variants in the SHANK3 gene. Individuals present with intellectual disability, autism-spectrum traits, seizures, gastrointestinal and motor issues, and sleep disturbances, requiring lifelong multidisciplinary care. In Italy, PMS care is fragmented and unevenly distributed, with families often providing intensive home-based support at high personal, financial, and social costs.

Methods

This national participatory Citizen-Science study, conducted with the Italian Phelan-McDermid Syndrome Association (AISPHEM), will engage informal caregivers of individuals with genetically confirmed PMS across Italy. A qualitative phase using semi-structured online interviews will explore caregiving experiences, unmet needs, barriers to care, coping strategies, and social isolation. Insights will guide the creation of the first Italian PMS registry, capturing longitudinal clinical, socio-demographic, and caregiver-related data.

Expected Results & Conclusions

The project will generate novel evidence on caregiver needs, develop the first national PMS registry, and produce a service map to support equitable, coordinated PMS care and a stronger national caregiver network in Italy.

phan - mcdermid综合征（PMS）是一种罕见的神经发育障碍，由染色体22q13.3缺失或SHANK3基因的致病性变异引起。患有智力残疾、自闭症谱系特征、癫痫、胃肠道和运动问题以及睡眠障碍的个体，需要终身多学科护理。在意大利，经前症候群护理支离破碎，分布不均，家庭往往以高昂的个人、经济和社会成本提供密集的家庭支持。方法：这项与意大利费伦-麦克德米德综合征协会（AISPHEM）合作进行的全国性参与性公民科学研究将招募意大利各地经遗传证实的经前症候群患者的非正式护理人员。使用半结构化在线访谈的定性阶段将探讨护理经验、未满足的需求、护理障碍、应对策略和社会孤立。洞察将指导创建第一个意大利经前症候群注册，捕获纵向临床，社会人口统计和护理相关数据。预期结果和结论该项目将提供有关护理人员需求的新证据，建立首个全国经前综合症登记处，并绘制服务地图，以支持意大利公平、协调的经前综合症护理和更强大的全国护理人员网络。

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引用次数: 0

An approach to in vivo low-magnification optical coherence tomography for selective monitoring of tissue glucose concentration 一种用于选择性监测组织葡萄糖浓度的体内低倍光学相干断层扫描方法

IF 1.9 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX

Pub Date : 2026-06-01 Epub Date: 2025-12-08 DOI: 10.1016/j.mex.2025.103752

Akitoshi Seiyama , Fengcheng Wei , Masato Ohmi

The low-magnification optical coherence tomography (LM-OCT) is expected to enable the quantification of tissue glucose concentration (TGC) by maintaining high spatial resolution while reducing artifacts caused by tissue heterogeneity. In the present study, we report the practical application of LM-OCT using a conventional OCT system, i.e., without compromising spatial resolution, for the selective monitoring of changes in TGC through in vivo experiments using a hairless rat model.

A commercially available high-spatial resolution OCT device with a center wavelength of 1300 nm was used to detect the three-dimensional (3D) skin surface structure and the scattering coefficient related to the OCT slope.

The grand-averaged OCT signal was employed to estimate the OCT slope, while the original high-resolution OCT signals were used to reconstruct the 3D-structural image.

A strong linear correlation was observed between changes in the OCT slope and that in TGC, but not with 2-deoxy-glucose, lactate, or Intralipos (soybean oil).

低倍率光学相干断层扫描（LM-OCT）有望通过保持高空间分辨率，同时减少由组织异质性引起的伪影，实现组织葡萄糖浓度（TGC）的量化。在本研究中，我们报告了LM-OCT在传统OCT系统的实际应用，即在不影响空间分辨率的情况下，通过无毛大鼠模型的体内实验，选择性地监测TGC的变化。利用市售的中心波长为1300 nm的高空间分辨率OCT装置检测皮肤的三维（3D）表面结构和与OCT斜率相关的散射系数。利用大平均OCT信号估计OCT斜率，利用原始高分辨率OCT信号重建三维结构图像。OCT斜率的变化与TGC有很强的线性相关性，但与2-脱氧葡萄糖、乳酸或脂肪内酯（大豆油）没有相关性。

引用次数: 0

Corrigendum to “From detection to grading: A hybrid KOA-YOLOv5-RF model for knee osteoarthritis diagnosis” [MethodsX, Volume 15, December 2025, 103725] “从检测到分级：一种混合KOA-YOLOv5-RF模型用于膝骨关节炎诊断”的勘误表[MethodsX， vol . 15, December 2025, 103725]

IF 1.9 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX

Pub Date : 2026-06-01 Epub Date: 2025-12-06 DOI: 10.1016/j.mex.2025.103751

Manikandaprabhu Perumalsamy , Priya Govindarajan , Rinhas Bran , Adarsh Krishna KP , Niranjan V Jyothi , M Batumalay

引用次数: 0

Optimizing blue poo: A validated, cost-effective method for measuring whole gut transit time 优化蓝色粪便：一种有效的，具有成本效益的方法来测量整个肠道运输时间

IF 1.9 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX

Pub Date : 2026-06-01 Epub Date: 2025-11-26 DOI: 10.1016/j.mex.2025.103741

Cyra Schmandt , Julia Trunz , Claudio Perret , Anneke Hertig-Godeschalk , Zeno Stanga , Jivko Stoyanov

Whole gut transit time (WGTT) provides essential insights into gastrointestinal health, but traditional measurement methods are often expensive or invasive. This study optimizes and validates the "blue dye method," an affordable and minimally invasive approach to WGTT measurement. Using "Hollinger Farbpulver Blau" (containing food colors E131 and E132), dye concentrations ranging from 30 mg to 241 mg were tested across four modes of delivery: capsule with liquid, gummy bear, muffin, and capsule with rice crackers and liquid. Each presented limitations: capsules taken with liquid led to inconsistent transit times, gummy bears caused staining, and muffins were perishable. Measured WGTTs varied between 18 and 29 h depending on the mode of delivery and dye concentration. Optimal protocol was a capsule containing 60 mg of dye taken with two rice crackers and liquid, ensuring accurate detection without practical inconveniences. The standardized and optimized blue dye method provides valid WGTT measurements, making it well suited for large-scale population studies and clinical applications.

Uses a simple blue dye as a marker for gut transit.

Tested several modes of delivery and concentrations to find the most practical option.

Established a standardized protocol for reliable and reproducible measurement.

全肠道传递时间（WGTT）提供了对胃肠道健康的重要见解，但传统的测量方法往往昂贵或具有侵入性。本研究优化并验证了“蓝色染料法”，这是一种经济实惠且微创的WGTT测量方法。使用“Hollinger Farbpulver Blau”（含有食用色素E131和E132），染料浓度从30毫克到241毫克，测试了四种递送模式：液体胶囊、小熊软糖、松饼胶囊和米饼和液体胶囊。每一种都有局限性：胶囊与液体一起服用会导致运输时间不一致，小熊软糖会引起染色，松饼容易变质。根据递送方式和染料浓度的不同，测得的wgtt在18至29小时之间变化。最佳方案为含60毫克染料的胶囊，用两个米饼和液体服用，确保准确检测而不带来实际不便。标准化和优化的蓝色染料方法提供了有效的WGTT测量，使其非常适合大规模人群研究和临床应用。用一种简单的蓝色染料作为肠道运输的标记。测试了几种给药方式和浓度，以找到最实用的选择。建立了可靠和可重复测量的标准化方案。

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引用次数: 0

Field boundary delineation with seasonal sentinel 2 imagery using Segment Anything Model (SAM) 利用分段任意模型（SAM）对季节性哨兵2影像进行野外边界圈定

IF 1.9 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX

Pub Date : 2026-06-01 Epub Date: 2026-01-09 DOI: 10.1016/j.mex.2026.103794

Thuan Ha, Kwabena Abrefa Nketia, Hansanee Fernando, Sarah van Steenbergen, Shawn Neudorf, Steve J. Shirtliffe

Accurate field boundary delineation is critical for accurate modelling on crop yields and for precision agriculture (PA), enabling site-specific management to optimize resource use and crop productivity. Traditional boundary mapping methods, such as manual digitization and semi-automated extraction from farm machinery, are labor-intensive and challenging to apply at large scales. Advances in high-resolution land cover data and satellite imagery offer scalable solutions for automated field boundary extraction. In this study, we propose a fully automated workflow that integrates a pre-trained foundation model, the Segment Anything Model - SAM [1] with time-series Sentinel-2 imagery. Seasonal composites of Red, Green, and Blue bands were generated at different phenological stages to support segmentation. The method was applied across over 32 million hectares (79 million acres) of cultivated land in the Canadian Prairies, achieving an intersection-over-union (IoU) accuracy of 0.86 compared to manual segmentation. The workflow consists of four main steps: (1) setting the python working environment, (2) seasonal image acquisition and preprocessing using Google Earth Engine via Python API; (3) field boundary segmentation using SAM; and (4) post-processing and feature cleaning using ArcGIS Pro. This approach demonstrates a scalable, efficient solution for large-scale field boundary mapping to support PA applications.

•
Integrates a foundation segmentation model (SAM) with Sentinel-2 seasonal imagery
•
Demonstrates high-accuracy, large-scale automated field boundary delineation
•
Provides a reproducible workflow adaptable to other regions and datasets

准确的田间边界划定对于精确模拟作物产量和精准农业（PA）至关重要，它使特定地点的管理能够优化资源利用和作物生产力。传统的边界制图方法，如人工数字化和农业机械的半自动化提取，是劳动密集型的，难以大规模应用。高分辨率土地覆盖数据和卫星图像的进步为自动化野外边界提取提供了可扩展的解决方案。在这项研究中，我们提出了一个完全自动化的工作流程，该工作流集成了预训练的基础模型，即片段任意模型- SAM[1]和时间序列Sentinel-2图像。在不同物候阶段生成红、绿、蓝波段的季节组合，以支持分割。该方法应用于加拿大大草原超过3200万公顷（7900万英亩）的耕地，与人工分割相比，实现了0.86的交叉-超联合（IoU）精度。该工作流程包括四个主要步骤：(1)设置python工作环境；(2)通过python API使用谷歌Earth Engine进行季节图像采集和预处理；(3)利用SAM进行野外边界分割；(4)利用ArcGIS Pro进行后处理和特征清理。这种方法展示了一种可扩展的、有效的解决方案，用于支持PA应用程序的大规模场边界映射。•将基础分割模型（SAM）与Sentinel-2季节性图像集成在一起•展示高精度，大规模自动化现场边界划定•提供可重复的工作流程，适用于其他地区和数据集

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引用次数: 0

Systematic review of gabion-faced geogrid and pile systems for slope and embankment stability 格宾网面土工格栅和桩系统对边坡和路堤稳定性的系统评价

IF 1.9 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX

Pub Date : 2026-06-01 Epub Date: 2025-12-17 DOI: 10.1016/j.mex.2025.103767

Devi Oktaviana Latif, Virananda Samudera Rahmadhian, Amalia Ula Hazhiyah

Slope instability is a major geotechnical hazard intensified by rainfall infiltration, seismic loading, groundwater fluctuations, and human disturbances. Composite reinforcement systems—such as gabion-faced geogrid walls combined with piles or geosynthetic-encased columns (GECs)—are increasingly implemented to address multi-hazard conditions. This study presents a PRISMA-guided systematic review of empirical, numerical, centrifuge, and field investigations on hybrid slope-stabilization systems. The review advances prior work by explicitly incorporating multi-field coupling and soil–structure interaction (SSI) terms into the search strategy, applying transparent screening and data-extraction procedures supported by a reusable metadata codebook, and conducting cross-study triangulation across field evidence, centrifuge modelling, and 2D/3D numerical analyses. The synthesized evidence shows that hybrid systems can significantly enhance slope performance, with reported improvements of up to ∼45 % in factor of safety and >30 % reduction in settlement, depending on reinforcement configuration, soil conditions, and coupled rainfall–seismic effects. The study further highlights current limitations in optimisation practice, long-term monitoring, and design standardisation, and outlines directions for uncertainty-aware and performance-based slope design.

边坡失稳是受降雨入渗、地震荷载、地下水波动和人为干扰加剧的主要岩土工程灾害。复合加固系统，如石笼面土工格栅墙与桩或土工合成材料包裹柱（gec）相结合，越来越多地用于解决多重灾害条件。本研究提出了prisma指导的经验，数值，离心机和混合边坡稳定系统的实地调查系统综述。该综述通过明确地将多场耦合和土壤-结构相互作用（SSI）术语纳入搜索策略，应用透明筛选和数据提取程序（由可重复使用的元数据代码本支持），以及跨场证据进行交叉研究三角测量，离心机建模和2D/3D数值分析，推进了先前的工作。综合证据表明，混合系统可以显著提高边坡性能，据报道，根据加固配置、土壤条件和降雨-地震耦合效应，混合系统的安全系数提高了45%，沉降减少了30%。该研究进一步强调了当前优化实践、长期监测和设计标准化方面的局限性，并概述了不确定性意识和基于性能的边坡设计的方向。

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引用次数: 0

Ex-situ growth protocol for the invasive macrophyte Pontederia crassipes 入侵植物庞德马的迁地生长方案

IF 1.9 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX

Pub Date : 2026-06-01 Epub Date: 2026-01-16 DOI: 10.1016/j.mex.2026.103800

Letícia da Silva Brito , Sidinei Magela Thomaz , Heliana Teixeira , Ana I. Lillebø

Pontederia crassipes is known for its asexual reproduction and rapid growth. Outside its native range, it has been identified as an environmental threat, while it has also been widely used for ex-situ phytoremediation. To understand both its invasive potential and its phytoremediation capacity, it is necessary to examine the environmental factors that favor its growth beyond those already described in the literature, such as water temperature and nutrient availability. Previous studies also suggest that alkalinity, conductivity, dissolved oxygen, salinity, water depth and pH. These variables help define the species niche and highlight the importance of distinguishing between its fundamental niche, the full set of abiotic conditions that support growth and its realized niche, which reflects biotic interactions and local constraints. However, the scientific literature does not yet provide sufficient description of the ex-situ experimental conditions required for the successful cultivation of this aquatic plant in controlled settings. This protocol therefore reports the results and lessons learned from a series of mesocosm experiments. By standardizing procedures and documenting growth outcomes, the protocol enhances reproducibility, facilitates comparisons across studies and supports both basic and applied research on P. crassipes.

Pontederia crassipes以其无性繁殖和快速生长而闻名。在其原生范围之外，它已被确定为环境威胁，同时它也被广泛用于异地植物修复。为了了解其入侵潜力和植物修复能力，有必要研究除了文献中已经描述的环境因素之外，有利于其生长的环境因素，如水温和养分有效性。先前的研究还表明，碱度、电导率、溶解氧、盐度、水深和ph值有助于定义物种生态位，并强调了区分其基本生态位、支持生长的全部非生物条件和实现生态位的重要性，这些生态位反映了生物相互作用和局部约束。然而，科学文献尚未对在受控环境下成功培养这种水生植物所需的迁地实验条件提供足够的描述。因此，本议定书报告了从一系列中观实验中获得的结果和经验教训。通过标准化的程序和记录生长结果，该协议提高了可重复性，促进了研究之间的比较，并支持了P. crassipes的基础和应用研究。

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引用次数: 0

The neurocultural remake reflex model: An interdisciplinary tool for understanding film remakes 神经文化重拍反射模型：理解电影重拍的跨学科工具

IF 1.9 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX

Pub Date : 2026-06-01 Epub Date: 2026-01-08 DOI: 10.1016/j.mex.2026.103786

Marziya Begum, Akaitab Mukherjee

The Neurocultural Remake Reflex Model (NRRM) is an interdisciplinary qualitative and neuroscience-informed analytical method that integrates heuristic insights from intersubject correlation (ISC) literature, cultural memory theory and affect theory to make reception-centered, testable inferences about how remakes trigger recognition, emotion and reinterpretation. The six-steps involved in the framework, Attention Mapping, Cognitive Processing, Cultural Recall, Cultural Reframing, Emotional Resonance and Emotional Dissonanceare operationalized through systematic qualitative scene analysis rather than empirical neuroimaging. Most existing research on remakes has primarily focused on comparative textual analysis, often overlooking the neurocognitive and emotional aspects of audience reception. The NRRM addresses this gap by explaining how viewers attend to, interpret, remember, emotionally connect with, and sometimes resist or contest a remake’s narrative and aesthetic cues. The paper demonstrates the method through a scene-level application of two case studies to show how remakes function simultaneously as mnemonic devices and affective tools. In doing so, NRRM reveals how remakes preserve cultural legacies while generating new meanings, offering film scholars, media psychologists and cultural analysts a reproducible framework for tracing how remakes conserve, reframe or disrupt cultural memory and affective experience.

This study proposes a six-step systematic qualitative methodology for analyzing remakes.

By integrating cognitive and cultural approaches, it introduces an interdisciplinary framework for remake analysis.

This framework demonstrates how remakes function as neurocultural reflexes, triggering nostalgia through both resonance and disruption.

神经文化重拍反射模型（NRRM）是一种跨学科的定性和神经科学分析方法，它整合了主体间相关（ISC）文献、文化记忆理论和情感理论的启发式见解，对重拍如何触发识别、情感和重新解释做出以接受为中心的可测试推论。注意映射、认知加工、文化回忆、文化重构、情绪共鸣和情绪失调这六个步骤是通过系统的定性场景分析而不是经验神经成像来实现的。大多数关于翻拍的研究主要集中在比较文本分析上，往往忽视了观众接受的神经认知和情感方面。NRRM通过解释观众如何关注、解释、记忆、情感联系，有时抵制或质疑翻拍的叙事和美学线索，来解决这一差距。本文通过两个案例的场景级应用来演示该方法，以展示重塑如何同时作为助记工具和情感工具。在此过程中，NRRM揭示了翻拍电影是如何保存文化遗产的同时产生新的意义，为电影学者、媒体心理学家和文化分析师提供了一个可复制的框架，以追踪翻拍电影是如何保存、重构或破坏文化记忆和情感体验的。本研究提出一套六步制的系统定性分析方法。通过整合认知和文化方法，引入了一个跨学科的重塑分析框架。这个框架展示了重塑如何作为神经文化反射，通过共鸣和破坏触发怀旧。

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引用次数: 0

Application of multiple parallel mineralization method to grow lettuce, Swiss chard, and peppers in a simplified deep flow technique hydroponic system 多重平行矿化法在简化深流技术水培系统中种植生菜、甜菜和辣椒的应用

IF 1.9 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX

Pub Date : 2026-06-01 Epub Date: 2025-12-06 DOI: 10.1016/j.mex.2025.103749

Jada Tarvin-Imeokparia , Adeyemi Abejoye , Isabella Vega , Max Abel Alphonse Mendoza , Campbell Moses , Myalia Durno , Meghan S. Cahill , Christian O. Dimkpa , Jason C. White , Shelley Durocher , Frederick Pettit , Mark Meyering , Jonathan M. Jacobs , Maria Soledad Benitez Ponce , Nathaniel Heiden

Organic fertilizers are not widely used in hydroponics due to an absence of mineralizing microbes that process organic inputs into plant available forms. The Multiple Parallel Mineralization (MPM) method enables this microbial processing to occur in hydroponic systems. The objective of this method is to provide an accessible protocol for use of the MPM method in simplified deep flow technique hydroponic systems without a need for laboratory testing of nutrient content. The method described here provides hydroponic growers with an alternative option to mineral fertilizers. We provide information about yields and the affordability of this method that growers can use to determine the economic viability of this method in their respective markets.

Swiss chard, lettuce, and bell peppers were grown with this method

Aquarium nitrate test kits are used to replace lab testing typically used with the MPM method to validate the presence of nitrate as an indicator of microbial activity

有机肥料在水培中没有广泛使用，因为没有矿化微生物将有机输入转化为植物可用的形式。多重平行矿化（MPM）方法使这种微生物处理发生在水培系统。该方法的目的是为在简化的深流技术水培系统中使用MPM方法提供一个可访问的协议，而无需实验室测试营养成分。这里描述的方法为水培种植者提供了矿物肥料的另一种选择。我们提供了有关产量和这种方法的可负担性的信息，种植者可以使用这些信息来确定这种方法在各自市场上的经济可行性。瑞士甜菜、生菜和甜椒都是用这种方法种植的。硝酸水族馆测试试剂盒用来取代通常用MPM方法进行的实验室测试，以验证硝酸盐作为微生物活动指标的存在

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引用次数: 0