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A randomized single-blinded, parallel-arm group feasibility trial evaluating role of pectoral nerve block on serum vascular endothelial growth factor levels in patients undergoing unilateral modified radical mastectomy. 一项随机单盲平行臂组可行性试验,评估胸神经阻滞对单侧改良根治性乳房切除术患者血清血管内皮生长因子水平的影响。
IF 3 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2020-10-01 DOI: 10.4103/2045-9912.299465
Nishith Govil, Manisha Naithani, Bina Ravi, Prateek Sharda, Mukesh Tripathi, Bharat Bhushan Bhardwaj

Metastatic breast cancer cells carry adult and neonatal variants of NaV1.5 voltage-gated activated Na+ channels involved in cell invasion. We hypothesize that instilling lignocaine near the surgical field to anesthetize the pectoral nerves for analgesia will decrease angiogenesis by blocking voltage-gated activated Na+ channels. Twenty patients undergoing unilateral modified radical mastectomy were randomized in a single-blinded, parallel-arm group feasibility pilot study in two groups. In Group I a catheter was placed between the pectoralis major and minor muscle under direct vision before skin closure. Ten milliliters of 2% lignocaine was given as an initial bolus followed by 10 mL of 2% lignocaine every 8 hours up to 24 hours. Group II did not receive any regional block. Primary measure outcomes were pre and postoperative changes in levels of vascular endothelial growth factor. Secondary outcomes were postoperative pain scores and total rescue analgesia used. Nine patients in each group were analyzed. Baseline demographic data of all females were similar with respect to age, body mass, height and duration of anesthesia. Postoperative mean serum levels of vascular endothelial growth factor were decreased by 46.60% from baseline in Group I, while were increased by 84.27% as compared to preoperative values in Group II. Postoperative average pain scores were less in Group I. Postoperative rescue analgesia in 24 hours in Group I was lower than that in Group II. There was no postoperative adverse event related to catheter or lignocaine administration at given doses. Instilling lignocaine to block pectoral nerves provides better postoperative analgesia and decreases a marker of angiogenesis. The study protocol was approved by the Institutional Ethical Committee of the Tertiary Centre (All India Institute of Medical Sciences Rishikesh India) (No. AIIMS/IEC/19/1002) on August 9, 2019, and the larger expansion trial was prospectively registered on Clinical Trial Registry India (No. CTRI/2020/01/022784) on January 15, 2020.

转移性乳腺癌细胞携带参与细胞侵袭的 NaV1.5 电压门控活化 Na+ 通道的成人和新生儿变体。我们假设,在手术野附近灌注木质素麻醉胸神经以达到镇痛目的,将通过阻断电压门控活化的 Na+ 通道来减少血管生成。在一项单盲平行臂组可行性试验研究中,20 名接受单侧改良根治性乳房切除术的患者被随机分为两组。第一组在皮肤闭合前,在直视下将导管置于胸大肌和胸小肌之间。首先注射 10 毫升 2% 的木质素,然后每 8 小时注射 10 毫升 2% 的木质素,直至 24 小时。第二组没有接受任何区域阻滞。主要测量结果为术前和术后血管内皮生长因子水平的变化。次要指标是术后疼痛评分和使用的镇痛抢救药物总量。每组有九名患者接受了分析。在年龄、体重、身高和麻醉持续时间方面,所有女性的基线人口统计学数据相似。第一组患者术后血清中血管内皮生长因子的平均水平比基线值降低了 46.60%,而第二组患者的血清中血管内皮生长因子的平均水平比术前值升高了 84.27%。第一组术后平均疼痛评分低于第二组。术后没有发生与导管或给药剂量的木质碱相关的不良事件。灌注木质素卡因阻断胸神经可提供更好的术后镇痛效果,并降低血管生成的一种标志物。该研究方案于2019年8月9日获得三级中心(印度全印度医学科学院里希凯什分院)机构伦理委员会批准(编号:AIIMS/IEC/19/1002),并于2020年1月15日在印度临床试验注册中心(编号:CTRI/2020/01/022784)对更大规模的扩展试验进行了前瞻性注册。
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引用次数: 0
Two-week continuous supplementation of hydrogenrich water increases peak oxygen uptake during an incremental cycling exercise test in healthy humans: a randomized, single-blinded, placebo-controlled study. 连续两周补充富氢水可提高健康人在增量自行车运动测试中的峰值摄氧量:一项随机、单盲、安慰剂对照研究。
IF 3 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2020-10-01 DOI: 10.4103/2045-9912.304223
Amane Hori, Sayaka Sobue, Ryosuke Kurokawa, Shin-Ichi Hirano, Masatoshi Ichihara, Norio Hotta

The various beneficial effects of the intake of molecular hydrogen (H2) have been demonstrated in the field of sports science. Although supplementation of H2 has been reported to increase mitochondrial metabolism in animal studies, the effects of the administration of H2 on aerobic capacity during exercise in humans are still not clear. We investigated whether a single or 2-week continuous intake of H2-rich water (HW) enhanced the aerobic capacity during incremental exercise in healthy humans. In this randomized, single-blinded, placebo-controlled experimental study, the participants performed an incremental cycling exercise to measure peak oxygen uptake and peak load before and after a single (500 mL) or a 2-week supplementation (total 5 L) of HW. In the latter experiment, the participants drank the 500 mL of HW on all weekdays (i.e., 10 times). The single intake of HW did not significantly increase peak oxygen uptake and peak load, and did not significantly alter the responses in oxidative stress, antioxidant activity, and lactate levels. However, importantly, the 2-week continuous consumption of HW significantly augmented peak oxygen uptake and tended to increase the peak load without any significant changes in lactate levels, oxidative stress, and antioxidant responses. In conclusion, the continuous supplementation of HW potentially augments the aerobic capacity, implying that continuous supplementation of H2 might help improve aerobic exercise performance and physical health. This study protocol was approved by the Ethical Committee of Chubu University (approval No. 260086-2) on March 29, 2018.

摄入分子氢(H2)的各种有益作用已在运动科学领域得到证实。虽然在动物实验中发现补充氢气能提高线粒体的新陈代谢,但服用氢气对人体运动中有氧能力的影响仍不清楚。我们研究了单次或连续两周摄入富含 H2 的水(HW)是否能增强健康人在增量运动中的有氧能力。在这项随机、单盲、安慰剂对照的实验研究中,参与者在单次(500 毫升)或连续 2 周(总计 5 升)补充 HW 之前和之后进行了增量自行车运动,以测量峰值摄氧量和峰值负荷。在后一项实验中,参与者在所有工作日(即 10 次)都饮用了 500 毫升 HW。单次摄入 HW 并没有明显增加峰值摄氧量和峰值负荷,也没有明显改变氧化应激、抗氧化活性和乳酸水平的反应。然而,重要的是,连续两周摄入 HW 能明显提高峰值摄氧量,并有增加峰值负荷的趋势,而乳酸水平、氧化应激和抗氧化反应却没有明显变化。总之,持续补充 HW 有可能增强有氧能力,这意味着持续补充 H2 可能有助于改善有氧运动表现和身体健康。本研究方案于2018年3月29日获得中部大学伦理委员会批准(批准号:260086-2)。
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引用次数: 0
Argon treatment after experimental subarachnoid hemorrhage: evaluation of microglial activation and neuronal survival as a subanalysis of a randomized controlled animal trial. 氩气治疗实验性蛛网膜下腔出血后:评价小胶质细胞活化和神经元存活作为一个随机对照动物试验的亚分析。
IF 2.9 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2020-07-01 DOI: 10.4103/2045-9912.296039
Benedikt Kremer, Mark Coburn, Agnieszka Weinandy, Kay Nolte, Hans Clusmann, Michael Veldeman, Anke Höllig

Hereinafter, we evaluate argon's neuroprotective and immunomodulatory properties after experimental subarachnoid hemorrhage (SAH) examining various localizations (hippocampal and cortical regions) with respect to neuronal damage and microglial activation 6, 24 and 72 hours after SAH. One hour after SAH (endovascular perforation rat model) or sham surgery, a mixture of gas containing 50% argon (argon group) or 50% nitrogen (control group) was applied for 1 hour. At 6 hours after SAH, argon reduced neuronal damage in the hippocampal regions in the argon group compared to the control group (P < 0.034). Hippocampal microglial activation did not differ between the treatment groups over time. The basal cortical regions did not show a different lesion pattern, but microglial activation was significantly reduced in the argon group 72 hours after SAH (P = 0.034 vs. control group). Whereas callosal microglial activation was significantly reduced at 24 hours in the argon-treated group (P = 0.018). Argon treatment ameliorated only early hippocampal neuronal damage after SAH. Inhibition of microglial activation was seen in some areas later on. Thus, argon may influence the microglial inflammatory response and neuronal survival after SAH; however, due to low sample sizes the interpretation of our results is limited. The study protocol was approved by the Government Agency for Animal Use and Protection (Protocol number: TVA 10416G1; initially approved by the "Landesamt für Natur, Umwelt und Verbraucherschutz NRW," Recklinghausen, Germany, on April 28, 2009).

接下来,我们评估氩气在实验性蛛网膜下腔出血(SAH)后的神经保护和免疫调节特性,检测不同部位(海马和皮质区域)在SAH后6、24和72小时的神经元损伤和小胶质细胞激活。在SAH(血管内穿孔大鼠模型)或假手术1小时后,给予含50%氩气(氩气组)或含50%氮气(对照组)的混合气体1小时。在SAH后6小时,氩气治疗组海马区神经元损伤较对照组明显减轻(P < 0.034)。随着时间的推移,海马小胶质细胞的激活在治疗组之间没有差异。基底皮质区没有表现出不同的病变模式,但在SAH后72小时,氩气组的小胶质细胞激活明显降低(P = 0.034)。而氩处理组在24小时时胼胝体小胶质细胞的激活显著降低(P = 0.018)。氩气治疗仅能改善SAH后早期海马神经元损伤。随后在一些区域发现了小胶质细胞活化的抑制。因此,氩气可能影响SAH后小胶质细胞的炎症反应和神经元存活;然而,由于样本量小,我们的结果的解释是有限的。本研究方案经政府动物使用和保护署批准(方案号:TVA 10416G1;2009年4月28日,德国Recklinghausen,“NRW自然,世界和Verbraucherschutz Landesamt fr nature, umwelund Verbraucherschutz”初步批准。
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引用次数: 8
Proposal of next-generation medical care "Mega-hydrogen Therapy". 下一代医疗保健“超级氢疗法”的建议。
IF 2.9 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2020-07-01 DOI: 10.4103/2045-9912.296045
Yusuke Ichikawa, Bunpei Satoh, Shin-Ichi Hirano, Ryosuke Kurokawa, Yoshiyasu Takefuji, Fumitake Satoh
Hydroxyl radicals in mitochondria: There are various types of reactive oxygen species (ROS) generated in mitochondria during cellular respiration, this is not an exaggeration that the ROS that causes cytotoxicity is only hydroxyl radical.2 Although humans have enzymes that scavenge some ROSs, we do not have enough enzymes that specifically can scavenge hydroxyl radical which is known to attack almost all components of cells and to cause aging and diseases. The inside of mitochondria is a place where various ROS are generated during respiration together with hydroxyl radicals that are also generated.3 In order to prevent and alleviate disease, it is important to scavenge hydroxyl radicals effectively not only at the cytoplasm, but also at the inside of organelles including mitochondria and cell nucleus. The important key issue is to establish a technique for scavenging hydroxyl radicals entirely in the matrix of mitochondria.
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引用次数: 1
Effects of hydrogen-rich water prepared by alternating-current-electrolysis on antioxidant activity, DNA oxidative injuries, and diabetes-related markers. 交变电流电解法制备的富氢水对抗氧化活性、DNA 氧化损伤和糖尿病相关指标的影响
IF 3 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2020-07-01 DOI: 10.4103/2045-9912.296041
Ryoko Asada, Kenji Tazawa, Shinkichi Sato, Nobuhiko Miwa

Hydrogen-rich water is conventionally prepared by direct current-electrolysis, but has been not or scarcely prepared by alternating current (AC)-electrolysis. The AC preparations from tap water for 20-30 minutes exhibit a dissolved hydrogen concentration of 1.55 mg/L, which was close to the theoretical maximum value of 1.6 mg/L. These preparations also displayed an oxidation-reduction potential of -270 mV (tap water: +576 mV) and pH of 7.7-7.8, being closer to physiological values of body fluids than general types of direct current-electrolytic hydrogen-rich water. We examined whether AC-electrolytic hydrogen-water is retained for hydrogen-abundance after boiling or for antioxidant abilities, and whether the oral administration of this water is clinically effective for diabetes and prevention against systemic DNA-oxidative injuries. 5,5-Dimethyl-1-pyrroline-N-oxide spin trapping and electron spin resonance revealed that the hydrogen-rich water generated by AC-electrolysis exhibited hydroxyl-radical-scavenging activities. Laser nanoparticle tracking method revealed that nanoparticle suspensions as abundant as 5.4 × 107/mL were efficiently retained (up to 3.5 × 107/mL) even after boiling for 10 minutes, being thermodynamically contrary to Henry's law. Oral intake of hydrogen-rich water, 1500 mL per day, lasted for 8 weeks in nine people with the diabetes-related serum markers beyond the normal ranges. The subjects exhibited significant tendencies for the decreased fasting blood glucose and fructosamine, and for the increased 1,5-anhydro-D-glucitol, concomitantly with significant decreases in urinary 8-hydroxy-2-deoxyguanosine contents and its rate of generation. Hydrogen-rich water prepared by AC-electrolysis may be effective in improving diverse diabetes-related markers and systemic DNA oxidative injuries through the formation of abundant heat-resistant nanobubbles and the increased hydrogen concentrations. The study protocol was officially approved by the Medical Ethics Committee of the Japanese Center for Anti-Aging Medical Sciences (approval No. 01S02) on September 15, 2009.

富氢水通常通过直流电解法制备,但很少或没有通过交流电解法制备。从自来水中提取 20-30 分钟的交流电制备的溶解氢浓度为 1.55 毫克/升,接近 1.6 毫克/升的理论最大值。这些制备物的氧化还原电位为 -270 mV(自来水:+576 mV),pH 值为 7.7-7.8,比一般类型的直流电解富氢水更接近体液的生理值。我们研究了交流电解氢水在煮沸后是否保留了氢的丰富性或抗氧化能力,以及口服这种水对糖尿病和预防全身 DNA 氧化损伤是否具有临床疗效。5,5-二甲基-1-吡咯啉-N-氧化物自旋阱和电子自旋共振显示,交流电解产生的富氢水具有清除羟基自由基的活性。激光纳米粒子跟踪法显示,纳米粒子悬浮液的含量高达 5.4 × 107 个/毫升,即使在沸腾 10 分钟后也能有效保留(高达 3.5 × 107 个/毫升),这在热力学上与亨利定律相悖。每天口服 1500 毫升富氢水,9 名糖尿病相关血清指标超出正常范围的人持续了 8 周。受试者的空腹血糖和果糖胺明显下降,1,5-脱水-D-葡萄糖醇明显增加,同时尿液中 8-羟基-2-脱氧鸟苷的含量及其生成率明显下降。通过交流电解制备的富氢水可形成大量耐热纳米气泡并增加氢浓度,从而有效改善多种糖尿病相关指标和全身 DNA 氧化损伤。研究方案于 2009 年 9 月 15 日获得日本抗衰老医学中心医学伦理委员会的正式批准(批准号:01S02)。
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引用次数: 0
Inhalation of molecular hydrogen increases breath acetone excretion during submaximal exercise: a randomized, single-blinded, placebo-controlled study. 在次极限运动中吸入氢分子增加呼吸丙酮排泄:一项随机、单盲、安慰剂对照研究。
IF 2.9 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2020-07-01 DOI: 10.4103/2045-9912.296038
Amane Hori, Masatoshi Ichihara, Hayata Kimura, Hisayoshi Ogata, Takaharu Kondo, Norio Hotta

Aerobic exercise is widely accepted as a beneficial option for reducing fat in humans. Recently, it has been suggested that molecular hydrogen (H2) augments mitochondrial oxidative phosphorylation. Therefore, the hypothesis that inhaling H2 could facilitate lipid metabolism during aerobic exercise was investigated in the current study by measuring the breath acetone levels, which could be used as non-invasive indicators of lipid metabolism. This study aimed to investigate the effect of inhaling H2 on breath acetone output during submaximal exercise using a randomized, single-blinded, placebo-controlled, and cross-over experimental design. After taking a 20-minute baseline measurement, breath acetone levels were measured in ten male subjects who performed a 60% peak oxygen uptake-intensity cycling exercise for 20 minutes while inhaling either 1% H2 or a control gas. In another experiment, six male subjects remained in a sitting position for 45 minutes while inhaling either 1% H2 or a control gas. H2 significantly augmented breath acetone and enhanced oxygen uptake during exercise (P < 0.01). However, it did not significantly change oxidative stress or antioxidant activity responses to exercise, nor did it significantly alter the breath acetone or oxygen uptake during prolonged resting states. These results suggest that inhaling H2 gas promotes an exercise-induced increase in hepatic lipid metabolism. The study was approved by the Ethical Committee of Chubu University, Japan (approved No. 260086-2) on March 29, 2018.

有氧运动被广泛认为是人类减少脂肪的有益选择。最近,有人提出分子氢(H2)增强线粒体氧化磷酸化。因此,本研究通过测量呼吸丙酮水平,探讨了有氧运动中吸入H2可以促进脂质代谢的假设,呼气丙酮水平可作为脂质代谢的无创指标。本研究采用随机、单盲、安慰剂对照和交叉实验设计,旨在研究吸入H2对亚极限运动中呼吸丙酮输出的影响。在进行了20分钟的基线测量后,对10名男性受试者进行了20分钟的呼吸丙酮水平测量,这些受试者在吸入1%氢气或对照气体的同时进行了60%峰值氧气吸收强度的自行车运动。在另一项实验中,六名男性受试者在吸入1%氢气或对照气体的同时保持坐姿45分钟。H2显著增加运动时呼吸丙酮和氧摄取(P < 0.01)。然而,它并没有显著改变运动后的氧化应激或抗氧化活性反应,也没有显著改变长时间静息状态下的呼吸丙酮或氧摄取。这些结果表明,吸入H2气体促进了运动诱导的肝脂质代谢的增加。本研究于2018年3月29日获得日本中部大学伦理委员会批准(批准号260086-2)。
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引用次数: 5
A narrative review of hydrogen oncology: from real world survey to real world evidence. 氢肿瘤学的叙事回顾:从真实世界的调查到真实世界的证据。
IF 2.9 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2020-07-01 DOI: 10.4103/2045-9912.296044
Ji-Bing Chen, You-Yong Lu, Ke-Cheng Xu

The use of hydrogen for cancer control has made great progress in cytology and animal experiments. With the increasing number of hydrogen products on the market, larger numbers of advanced cancer patients have participated in clinical trials or received treatment at home after purchase. Our study reported a real-world survey from 82 patients with good cancer control using hydrogen products, including real world evidence from patients who received ineffective traditional treatment, patients who received traditional treatment that failed, or patients who refused traditional treatment. Two typical cases were reported herein. Subsequently, we included studies on the mechanism of hydrogen oncology. The mechanism of cancer control using hydrogen includes the inhibition of tumor cells and the activation of exhausted lymphocytes. Large-scale real world evidence has shown clinical value, and yet remains to be further developed and researched.

利用氢气控制癌症在细胞学和动物实验方面取得了很大进展。随着市场上氢产品的增多,越来越多的晚期癌症患者在购买后参与临床试验或在家接受治疗。我们的研究报告了对82名使用氢产品的癌症控制良好的患者的真实世界调查,包括接受传统治疗无效的患者,接受传统治疗失败的患者或拒绝传统治疗的患者的真实世界证据。本文报告两例典型病例。随后,我们纳入了氢肿瘤机制的研究。氢对肿瘤的控制机制包括抑制肿瘤细胞和激活衰竭淋巴细胞。大规模的真实世界证据已经显示出临床价值,但仍有待进一步开发和研究。
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引用次数: 4
Rationale for ozone-therapy as an adjuvant therapy in COVID-19: a narrative review. 臭氧治疗作为COVID-19辅助治疗的基本原理:叙述性回顾。
IF 2.9 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2020-07-01 DOI: 10.4103/2045-9912.289462
Giovanni Tommaso Ranaldi, Emanuele Rocco Villani, Laura Franza

Coronavirus disease 2019 (COVID-19) is the respiratory disease caused by the novel severe acute respiratory syndrome-coronavirus-2 and is characterized by clinical manifestations ranging from mild, flu-like symptoms to severe respiratory insufficiency and multi-organ failure. Patients with more severe symptoms may require intensive care treatments and face a high mortality risk. Also, thrombotic complications such as pulmonary embolisms and disseminated intravascular coagulation are frequent in these patients. Indeed, COVID-19 is characterized by an abnormal inflammatory response resembling a cytokine storm, which is associated to endothelial dysfunction and microvascular complications. To date, no specific treatments are available for COVID-19 and its life-threatening complication. Immunomodulatory drugs, such as hydroxychloroquine and interleukin-6 inhibitors, as well as antithrombotic drugs such as heparin and low molecular weight heparin, are currently being administered with some benefit. Ozone therapy consists in the administration of a mixture of ozone and oxygen, called medical ozone, which has been used for over a century as an unconventional medicine practice for several diseases. Medical ozone rationale in COVID-19 is the possibility of contrasting endothelial dysfunction, modulating the immune response and acting as a virustatic agent. Thus, medical ozone could help to decrease lung inflammation, slow down viral growth, regulate lung circulation and oxygenation and prevent microvascular thrombosis. Ozone-therapy could be considered a feasible, cost-effective and easy to administer adjuvant therapy while waiting for the synthesis of a therapy or the development of the vaccine.

2019冠状病毒病(COVID-19)是由新型严重急性呼吸综合征-冠状病毒-2引起的呼吸道疾病,临床表现为轻微流感样症状到严重呼吸功能不全和多器官衰竭。症状较严重的患者可能需要重症监护治疗,并面临较高的死亡风险。此外,血栓并发症如肺栓塞和弥散性血管内凝血在这些患者中也很常见。事实上,COVID-19的特征是类似于细胞因子风暴的异常炎症反应,这与内皮功能障碍和微血管并发症有关。迄今为止,没有针对COVID-19及其危及生命的并发症的特定治疗方法。免疫调节药物,如羟氯喹和白细胞介素-6抑制剂,以及抗血栓药物,如肝素和低分子量肝素,目前正在使用,并有一些益处。臭氧疗法包括使用臭氧和氧气的混合物,称为医用臭氧,作为一种治疗几种疾病的非常规医学实践已经使用了一个多世纪。在COVID-19中,臭氧的医学原理是对比内皮功能障碍、调节免疫反应和作为抗病毒药物的可能性。因此,医用臭氧可以减轻肺部炎症,减缓病毒生长,调节肺部循环和氧合,防止微血管血栓形成。在等待合成一种疗法或研制出疫苗期间,臭氧疗法可被认为是一种可行、具有成本效益和易于实施的辅助疗法。
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引用次数: 10
Ozone therapy for the treatment of recurrent pigmented villonodular synovitis of the knee. 臭氧疗法治疗复发性膝色素绒毛结节性滑膜炎。
IF 2.9 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2020-07-01 DOI: 10.4103/2045-9912.296046
Hasan Oguz, Mustafa Turgut Yildizgoren
Dear Editor, Pigmented villonodular synovitis (PVNS) is a benign proliferative condition that develops in the synovial membranes of joints, bursa or tendon sheaths most frequently in the knee joints. The patient usually has mono-articular pain and swelling. Aspiration of the joint characteristically reveals blood-tinged fluid. The synovial tissue has a characteristic brownish discoloration due to hemosiderin deposits. As the condition reaches an advanced stage, erosive lesions can be detected in the adjacent bony structures. As the weight-bearing extremities are most prone to this, the knee joint (70%) is particularly affected, although there can be involvement of the ankle, shoulder, wrist, and other joints. Patients usually complain of progressive joint swelling and discomfort with insidious onset. PVNS can occur in all age groups, but those aged 20–50 years are the most frequently affected.1 Plain radiographs usually show non-specific features such as bony erosions or soft tissue swelling. Computed tomography and ultrasonography can also show the hypertrophic synovium as a slightly hyper dense/echogenic soft-tissue mass. In the differentiation of PVNS from other synovial diseases, magnetic resonance imaging is of benefit as hyperplastic synovium or localized mass lesions are indicated by an abnormally low signal intensity on both T1 and T2 weighed images. Magnetic resonance imaging has the advantages of showing mass-like synovial proliferation with lobulated margins, with low signal intensity and hemosiderin deposits seen as “blooming” artifact on gradient echo.2 Here, the case is described of a patient with a history of knee pain and swelling, who was diagnosed with localized PVNS, and was treated successfully with local ozone therapy. This is the first case which describes treatment with ozone of PVNS in a patient refractory to surgical treatment. A 30-year old female presented with complaints of pain and swelling in the left knee which had been ongoing for 3 years. Initial assessment of pain was 7 on the Numeric Rating Scale. The patient had a history of arthroscopic synoviectomy 10 years previously because of PVNS and the pain was relieved after surgery. On examination, the range of motion of the knee joint was limited to 120° of active flexion, with full extension. Laboratory test results showed: C-reactive protein = 2 mg/L (normal range: 0–5 mg/L), erythrocyte sedimentation rate = 15 mm/h (normal range: 0–20 mm/h), rheumatoid factor = 9 IU/mL (normal range: 0–20 IU/mL), anti-cyclic citrullinated peptide = 0.5 U/mL (normal range: 0–20 U/mL). In addition, complete blood count, hepatic panel, blood urea nitrogen and creatinine levels were within normal limits. Radiographs were normal. Magnetic resonance imaging showed a 22 mm × 12 mm hypodense nodular lesion around the posterior longitudinal ligament (Figure 1A). PVNS was diagnosed based on these findings. The patient was treated with arthroscopic plica excision and synoviectomy. Two months
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引用次数: 1
The impact of carbon monoxide inhalation on developing noise-induced hearing loss in guinea pigs. 吸入一氧化碳对豚鼠发生噪音性听力损失的影响。
IF 2.9 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Pub Date : 2020-07-01 DOI: 10.4103/2045-9912.296040
Fereshte Bagheri, Mahbubeh Sheikhzadeh, Ahmadreza Raisi, Mohammad Kamali, Mohammad Faridan

Carbon monoxide (CO) poisoning is one of the most common types of fatal poisonings worldwide. Acute exposure to high levels of CO as well as chronic exposure to low levels of CO and excessive noise can lead to high frequency hearing loss. In this study, twelve guinea pigs were randomly divided into two groups: (1) exposed to noise and (2) exposed to noise plus CO. Auditory brainstem responses (ABRs) were measured prior to the experiment and immediately, 5, 10 and 15 days post exposures. There was a significant difference between the ABR thresholds before and immediately after exposure to noise at frequencies of 4, 8, and 16 kHz and the most threshold shift was observed at 8 kHz. There was also a significant difference between the ABR thresholds before and immediately after exposure to noise and CO at frequencies of 2, 4, 8, and 16 kHz which demonstrated a temporary hearing loss after exposure to noise and CO and the major impact of CO on developing noise induced hearing loss occurred at 8 kHz. No significant difference was observed between the ABR thresholds recorded before conducting the experiments and the ones obtained 5, 10 and 15 days after simultaneous exposure to noise and CO at none of frequencies. Simultaneous exposure to noise and CO contributes to transient hearing loss in guinea pigs with the most evident temporary shift at 8 kHz. The methods were accepted in the Ethics Committee of Iran University of Medical Science (registration No. CTRI/2016/01/017170) on January 18, 2016.

一氧化碳中毒是世界上最常见的致命中毒之一。急性暴露于高水平的一氧化碳以及长期暴露于低水平的一氧化碳和过度的噪音可导致高频听力丧失。本研究将12只豚鼠随机分为两组:(1)噪声暴露组和(2)噪声加CO暴露组。分别在实验前、实验后即刻、实验后5、10和15天测量听觉脑干反应(ABRs)。4、8和16 kHz噪声暴露前后的ABR阈值有显著差异,8 kHz时阈值移位最多。在2、4、8和16 kHz的频率下,接触噪音和一氧化碳前后的ABR阈值也存在显著差异,这表明在接触噪音和一氧化碳后会出现暂时的听力损失,而CO对发生噪音性听力损失的主要影响发生在8 kHz。实验前记录的ABR阈值与同时暴露于噪声和CO的5、10和15天后的ABR阈值之间没有显著差异。同时暴露于噪音和一氧化碳会导致豚鼠短暂性听力丧失,在8千赫时最明显的暂时性移位。这些方法已被伊朗医科大学伦理委员会接受(注册号:CTRI/2016/01/017170),于2016年1月18日发布。
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引用次数: 0
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Medical Gas Research
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