Pub Date : 2022-06-15DOI: 10.1088/2050-6120/ac7943
Karolina Maleckaitė, Jelena Dodonova-Vaitkūnienė, Rugilė Žilėnaitė, S. Tumkevičius, Aurimas Vyšniauskas
Microviscosity has a strong impact for diffusion-controlled processes in biological environments. BODIPY molecular rotors are viscosity-sensitive fluorophores that provide a simple and non-invasive way to visualise microviscosity. Although green fluorescent probes are already well developed for imaging, thick biological samples require longer wavelengths for investigation. This work focuses on the examination of novel β-substituted meso-phenyl-BODIPYs possessing a red emission. We report a new red fluorescent BODIPY-based probe BP-Vinyl-NO2 suitable for sensing microviscosity in rigid environments of over 100 000 cP viscosities. Furthermore, we demonstrate that changing the methyl position from ortho to meta on the β-phenyl-substituted conjugate BP-PH-m2M-NO2 redshifts absorbance and fluorescence spectra while maintaining viscosity sensitivity. Finally, we show that nitro-substitution of meso-phenyl is a versatile approach to improve the sensitivity to viscosity while suppressing sensitivity to polarity and temperature of such derivatives. In summary, we present two nitro-substituted red fluorescent probes that could be used as lifetime-based microviscosity sensors.
{"title":"Red fluorescent BODIPY molecular rotor for high microviscosity environments","authors":"Karolina Maleckaitė, Jelena Dodonova-Vaitkūnienė, Rugilė Žilėnaitė, S. Tumkevičius, Aurimas Vyšniauskas","doi":"10.1088/2050-6120/ac7943","DOIUrl":"https://doi.org/10.1088/2050-6120/ac7943","url":null,"abstract":"Microviscosity has a strong impact for diffusion-controlled processes in biological environments. BODIPY molecular rotors are viscosity-sensitive fluorophores that provide a simple and non-invasive way to visualise microviscosity. Although green fluorescent probes are already well developed for imaging, thick biological samples require longer wavelengths for investigation. This work focuses on the examination of novel β-substituted meso-phenyl-BODIPYs possessing a red emission. We report a new red fluorescent BODIPY-based probe BP-Vinyl-NO2 suitable for sensing microviscosity in rigid environments of over 100 000 cP viscosities. Furthermore, we demonstrate that changing the methyl position from ortho to meta on the β-phenyl-substituted conjugate BP-PH-m2M-NO2 redshifts absorbance and fluorescence spectra while maintaining viscosity sensitivity. Finally, we show that nitro-substitution of meso-phenyl is a versatile approach to improve the sensitivity to viscosity while suppressing sensitivity to polarity and temperature of such derivatives. In summary, we present two nitro-substituted red fluorescent probes that could be used as lifetime-based microviscosity sensors.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43344188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-15DOI: 10.1088/2050-6120/ac7944
Mengting Zhang, Yulu Zhang, Mingyu Gan, Liping Xie, Jing Wang, Wei‐Hua Jia, W. Bian, S. Shuang, Martin M. F. Choi
A fluorescent sulfur and oxygen co-doped graphitic carbon nitride quantum dots (S,O-CNQDs) were prepared from ethylenediaminetetraacetic acid disodium salt dihydrate and thiourea as the carbon and sulfur sources. The morphology and surface functional groups of S,O-CNQDs were characterized by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. The fluorescence of S,O-CNQDs could be quenched efficiently by Cu2+ under the optimum conditions. The S,O-CNQDs could function as an excellent fluorescent probe for Cu2+ detection with a wide linear range of 0.50–15 μM and a low detection limit of 0.58 nM. In addition, this fluorescent probe was employed for monitoring Cu2+ in samples of tap water, lake water, human serum and urine with good recoveries from 99.0% to 110.0%. Moreover, the S,O-CNQDs with high cell penetration and low cytotoxicity were utilized for Cu2+ detection in living cells. Owing to the excellent properties of S,O-CNQDs, the as-prepared S,O-CNQDs can be a potential candidate for biological applications.
{"title":"Facile synthesis of sulfur and oxygen co-doped graphitic carbon nitride quantum dots for on-off detection of Cu2+ in real samples and living cells","authors":"Mengting Zhang, Yulu Zhang, Mingyu Gan, Liping Xie, Jing Wang, Wei‐Hua Jia, W. Bian, S. Shuang, Martin M. F. Choi","doi":"10.1088/2050-6120/ac7944","DOIUrl":"https://doi.org/10.1088/2050-6120/ac7944","url":null,"abstract":"A fluorescent sulfur and oxygen co-doped graphitic carbon nitride quantum dots (S,O-CNQDs) were prepared from ethylenediaminetetraacetic acid disodium salt dihydrate and thiourea as the carbon and sulfur sources. The morphology and surface functional groups of S,O-CNQDs were characterized by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. The fluorescence of S,O-CNQDs could be quenched efficiently by Cu2+ under the optimum conditions. The S,O-CNQDs could function as an excellent fluorescent probe for Cu2+ detection with a wide linear range of 0.50–15 μM and a low detection limit of 0.58 nM. In addition, this fluorescent probe was employed for monitoring Cu2+ in samples of tap water, lake water, human serum and urine with good recoveries from 99.0% to 110.0%. Moreover, the S,O-CNQDs with high cell penetration and low cytotoxicity were utilized for Cu2+ detection in living cells. Owing to the excellent properties of S,O-CNQDs, the as-prepared S,O-CNQDs can be a potential candidate for biological applications.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45328556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-13DOI: 10.1088/2050-6120/ac784d
Sharmistha Das, Shirsendu Das, A. Singh, A. Datta
3-Aminoquinoline (3AQ) has been used as a fluorescent probe for preferential solvation in hexane-ethanol solvent mixtures. Results of the present experiment have been put into context by comparison with prior observations with 5-aminoquinoline (5AQ) as the probe. 3AQ exhibits a relatively small change of dipole moment (Δμ = 2.2 D) upon photoexcitation, compared to 5AQ (Δμ = 6.1D), which might appear to be a hindrance in the way of its use as a solvation probe. Indeed, the values of parameters like spectral shifts are smaller for the present experiment with 3AQ. At the smallest concentration of alcohol used, its local mole fraction around the probe is significantly lower than in the previous experiments with 5AQ. However, these apparent disadvantages are outweighed by the significant increase in fluorescence intensity and lifetime observed with increasing concentration of ethanol in the solvent mixture, as opposed to the drastic fluorescence quenching that occurs for 5AQ. This is a marked advantage in the use of 3AQ in studies like the present one. The local mole fraction of ethanol and preferential solvation index experienced by 3AQ are in line with those reported for 5AQ. The disadvantage of the smaller magnitude of Δμ persists in the time resolved fluorescence experiments, for solvent mixtures with very low ethanol content. Negligible wavelength dependence of fluorescence transients of 3AQ is observed for x p = 0.002,. However, this effect is outweighed at higher alcohol concentrations, for which nanosecond dynamics of preferential solvation is observed.
用3-氨基喹啉(3AQ)作为荧光探针，在己烷-乙醇混合溶剂中优先溶剂化。将实验结果与先前以5-氨基喹啉(5AQ)为探针的观察结果进行了比较。与5AQ (Δμ = 6.1D)相比，3AQ在光激发下的偶极矩变化相对较小(Δμ = 2.2 D)，这可能会阻碍其作为溶剂化探针的使用。事实上，在目前的3AQ实验中，谱移等参数的值更小。在最小酒精浓度下，其在探针周围的局部摩尔分数明显低于先前使用5AQ的实验。然而，随着溶剂混合物中乙醇浓度的增加，荧光强度和寿命显著增加，而不是发生在5AQ中的剧烈荧光猝灭，这些明显的缺点被抵消了。在像现在这样的研究中使用3AQ是一个明显的优势。3AQ的局部乙醇摩尔分数和优先溶剂化指数与5AQ一致。在时间分辨荧光实验中，对于乙醇含量非常低的溶剂混合物，仍然存在Δμ量级较小的缺点。在x p = 0.002时，观察到3AQ荧光瞬态的波长依赖性可以忽略不计。然而，在较高的酒精浓度下，这种效应被抵消了，因为可以观察到纳秒级的优先溶剂化动力学。
{"title":"3-aminoquinoline: a turn-on fluorescent probe for preferential solvation in binary solvent mixtures","authors":"Sharmistha Das, Shirsendu Das, A. Singh, A. Datta","doi":"10.1088/2050-6120/ac784d","DOIUrl":"https://doi.org/10.1088/2050-6120/ac784d","url":null,"abstract":"3-Aminoquinoline (3AQ) has been used as a fluorescent probe for preferential solvation in hexane-ethanol solvent mixtures. Results of the present experiment have been put into context by comparison with prior observations with 5-aminoquinoline (5AQ) as the probe. 3AQ exhibits a relatively small change of dipole moment (Δμ = 2.2 D) upon photoexcitation, compared to 5AQ (Δμ = 6.1D), which might appear to be a hindrance in the way of its use as a solvation probe. Indeed, the values of parameters like spectral shifts are smaller for the present experiment with 3AQ. At the smallest concentration of alcohol used, its local mole fraction around the probe is significantly lower than in the previous experiments with 5AQ. However, these apparent disadvantages are outweighed by the significant increase in fluorescence intensity and lifetime observed with increasing concentration of ethanol in the solvent mixture, as opposed to the drastic fluorescence quenching that occurs for 5AQ. This is a marked advantage in the use of 3AQ in studies like the present one. The local mole fraction of ethanol and preferential solvation index experienced by 3AQ are in line with those reported for 5AQ. The disadvantage of the smaller magnitude of Δμ persists in the time resolved fluorescence experiments, for solvent mixtures with very low ethanol content. Negligible wavelength dependence of fluorescence transients of 3AQ is observed for x p = 0.002,. However, this effect is outweighed at higher alcohol concentrations, for which nanosecond dynamics of preferential solvation is observed.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44887679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-19DOI: 10.1088/2050-6120/ac7199
Jia Li, Cuiping Zhou, Hong Yang, Xiongzhi Wu, Liqiang Yan
Zinc (Zn2+) and lead (Pb2+) ions in the environment have important effects on human health and environmental safety. Therefore, it is necessary to effectively detect them by a convenient and reliable analysis method. In this study, two near-infrared fluorescent probes for the fast determination of Zn2+ and Pb2+ were synthesized by a simple Schiff base reaction between the dicyanoisophorone skeleton and carbohydrazide derivatives. Among them, the probe with the thiophene-2-carbohydrazide group showed a selective fluorescence response to Zn2+ and Pb2+ with a maximum emission wavelength of 670 nm. And the detection limits of the probe for Zn2+ and Pb2+ were 1.59 nM and 1.65 nM, respectively. In contrast the probe modified by the furan-2-carbohydrazide group achieved quantitative detection of Zn2+, with a detection limit of 2.7 nM. These results were attributed to the fact that the probes bind to Zn2+ and Pb2+ in stoichiometric ratios of 1:1, blocking the intramolecular PET effect. Furthermore, these two probes can be recycled through the action of EDTA and have been successfully used to detect Zn2+ and Pb2+ in real water samples.
{"title":"Two near-infrared fluorescent probes based on dicyanoisfluorone for rapid monitoring of Zn2+ and Pb2+","authors":"Jia Li, Cuiping Zhou, Hong Yang, Xiongzhi Wu, Liqiang Yan","doi":"10.1088/2050-6120/ac7199","DOIUrl":"https://doi.org/10.1088/2050-6120/ac7199","url":null,"abstract":"Zinc (Zn2+) and lead (Pb2+) ions in the environment have important effects on human health and environmental safety. Therefore, it is necessary to effectively detect them by a convenient and reliable analysis method. In this study, two near-infrared fluorescent probes for the fast determination of Zn2+ and Pb2+ were synthesized by a simple Schiff base reaction between the dicyanoisophorone skeleton and carbohydrazide derivatives. Among them, the probe with the thiophene-2-carbohydrazide group showed a selective fluorescence response to Zn2+ and Pb2+ with a maximum emission wavelength of 670 nm. And the detection limits of the probe for Zn2+ and Pb2+ were 1.59 nM and 1.65 nM, respectively. In contrast the probe modified by the furan-2-carbohydrazide group achieved quantitative detection of Zn2+, with a detection limit of 2.7 nM. These results were attributed to the fact that the probes bind to Zn2+ and Pb2+ in stoichiometric ratios of 1:1, blocking the intramolecular PET effect. Furthermore, these two probes can be recycled through the action of EDTA and have been successfully used to detect Zn2+ and Pb2+ in real water samples.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2022-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46908505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A novel fluorescent probe TSOC (thiazole salicylaldehyde oxazole chlorinated) was synthesized based on benzothiazole conjugated olefinic bonds with salicylicaldehyde unit as fluorophore and a phenyl oxazole unit as bonding unit. The probe could reversibly detect of Cu2+ and S2− over other common ions with longer emission and large stokes shift in an aqueous solution at pH 7.3 (DMSO-Hepes, v/v, 5:1, 10 mM). The bonding mechanism was supported through the titration experiment of fluorescence and absorption spectroscopy, 1H-NMR titration, HR-MS and DFT calculations. Moreover, the probe further exhibited good cell permeability and were successfully used to visualize Cu2+ and S2− in living cells.
{"title":"A novel reversible fluorescent probe for Cu2+ and S2− ions and imaging in living cells","authors":"Shuaici Cheng, Ran Sun, Zhuo-Hui Wu, Huihui Mei, Haocheng Yang, Qingqing Kong, Kuoxi Xu","doi":"10.1088/2050-6120/ac719a","DOIUrl":"https://doi.org/10.1088/2050-6120/ac719a","url":null,"abstract":"A novel fluorescent probe TSOC (thiazole salicylaldehyde oxazole chlorinated) was synthesized based on benzothiazole conjugated olefinic bonds with salicylicaldehyde unit as fluorophore and a phenyl oxazole unit as bonding unit. The probe could reversibly detect of Cu2+ and S2− over other common ions with longer emission and large stokes shift in an aqueous solution at pH 7.3 (DMSO-Hepes, v/v, 5:1, 10 mM). The bonding mechanism was supported through the titration experiment of fluorescence and absorption spectroscopy, 1H-NMR titration, HR-MS and DFT calculations. Moreover, the probe further exhibited good cell permeability and were successfully used to visualize Cu2+ and S2− in living cells.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2022-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45128759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-18DOI: 10.1088/2050-6120/ac7123
Han Dong, Mogos Girmatsion, Ruoyu Wang, Gang Lu, Yunfei Xie, Yahui Guo, H. Qian, Weirong Yao
Chemical pollutants such as heavy metals and antibiotics in the environment pose a huge threat to humans and animals. Our studies have demonstrated that the fluorescence of phycocyanin showed quenching responses towards both mercury (Hg2+) and ciprofloxacin (CIP), which acted in accordance with the ‘OR’ molecular logic gate. In order to discriminate Hg2+ and CIP in application scenarios, cysteine (Cys) was utilized to design another ‘INHIBIT’ logic gate, in which Hg2+ and Cys were the two inputs. Thus, an intelligent biosensor with dual-target identification capacity was successfully developed by using a fluorescent natural protein in an ingenious logic gate system.
{"title":"Construction of fluorescent logic gates for the detection of mercury(II) and ciprofloxacin based on phycocyanin","authors":"Han Dong, Mogos Girmatsion, Ruoyu Wang, Gang Lu, Yunfei Xie, Yahui Guo, H. Qian, Weirong Yao","doi":"10.1088/2050-6120/ac7123","DOIUrl":"https://doi.org/10.1088/2050-6120/ac7123","url":null,"abstract":"Chemical pollutants such as heavy metals and antibiotics in the environment pose a huge threat to humans and animals. Our studies have demonstrated that the fluorescence of phycocyanin showed quenching responses towards both mercury (Hg2+) and ciprofloxacin (CIP), which acted in accordance with the ‘OR’ molecular logic gate. In order to discriminate Hg2+ and CIP in application scenarios, cysteine (Cys) was utilized to design another ‘INHIBIT’ logic gate, in which Hg2+ and Cys were the two inputs. Thus, an intelligent biosensor with dual-target identification capacity was successfully developed by using a fluorescent natural protein in an ingenious logic gate system.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2022-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43880530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-17DOI: 10.1088/2050-6120/ac70ab
Vishab Kesarwani, V. K. Rai
The present article reports the optical absorption and upconversion (UC) studies of 1.0 mol% Er3+/2.0 mol% Yb3+ doped/codoped glasses prepared by melt-quenching technique. The elements present and the composition of the prepared glass have been confirmed from XPS and XRF analysis respectively. Judd-Ofelt intensity parameters have been calculated using the absorption spectrum which is further utilized to predict the nature of Er_O bond, the transition probabilities, branching ratios and radiative lifetimes. The CIE study shows non-colour tunable and highly pure green emission (94.2%). The temperature-dependent UC emission spectra of the 2.0 mol% Yb3+ sensitized glass have been recorded at three different pump power densities to establish a reliable FIR based temperature scale. Furthermore, the Arrhenius fitting of the temperature-dependent spectra reveals low thermal quenching of green luminescence in the codoped glass.
{"title":"Fluorescence intensity ratio technique and its reliability","authors":"Vishab Kesarwani, V. K. Rai","doi":"10.1088/2050-6120/ac70ab","DOIUrl":"https://doi.org/10.1088/2050-6120/ac70ab","url":null,"abstract":"The present article reports the optical absorption and upconversion (UC) studies of 1.0 mol% Er3+/2.0 mol% Yb3+ doped/codoped glasses prepared by melt-quenching technique. The elements present and the composition of the prepared glass have been confirmed from XPS and XRF analysis respectively. Judd-Ofelt intensity parameters have been calculated using the absorption spectrum which is further utilized to predict the nature of Er_O bond, the transition probabilities, branching ratios and radiative lifetimes. The CIE study shows non-colour tunable and highly pure green emission (94.2%). The temperature-dependent UC emission spectra of the 2.0 mol% Yb3+ sensitized glass have been recorded at three different pump power densities to establish a reliable FIR based temperature scale. Furthermore, the Arrhenius fitting of the temperature-dependent spectra reveals low thermal quenching of green luminescence in the codoped glass.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"10 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2022-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60499051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-17DOI: 10.1088/2050-6120/ac70aa
J. Schuyt, G. Williams, K. Shinohara, T. Shimizu, K. Yamanoi, M. Cadatal-Raduban
The luminescence properties of NaMgF3:Sm and NaMgF3:Ce,Sm were studied in the vacuum ultraviolet spectral region. Excitation bands corresponding to the charge transfer processes F− → Sm3+, O2− → Sm3+, and O2− → Ce3+, and the energy transfer processes Ce3+ → Sm3+ and O2− → Sm3+, were observed. The energies of the Sm3+ charge transfer transitions and the crystal field split Ce3+ 4f 05d 1 transitions were used to construct a complete host referred binding energy diagram for the series of lanthanide-doped NaMgF3:Ln compounds. We demonstrate that the optical and luminescence properties predicted by the binding energy diagram are in good agreement with those predicted by the binding energy diagram constructed via the alternative impurity-informed method, and all available experimental data regarding the NaMgF3:Ln compounds. We demonstrate that NaMgF3:Ln compounds are model systems for the study of charge trapping phenomena and divalent lanthanide luminescence. Ultimately, we validate that the impurity-informed method can be used to establish the energy levels of lanthanides in fluoride systems.
{"title":"Vacuum ultraviolet photoluminescence of NaMgF3:Sm and NaMgF3:Sm,Ce: energy levels of the lanthanides in NaMgF3:Ln compounds","authors":"J. Schuyt, G. Williams, K. Shinohara, T. Shimizu, K. Yamanoi, M. Cadatal-Raduban","doi":"10.1088/2050-6120/ac70aa","DOIUrl":"https://doi.org/10.1088/2050-6120/ac70aa","url":null,"abstract":"The luminescence properties of NaMgF3:Sm and NaMgF3:Ce,Sm were studied in the vacuum ultraviolet spectral region. Excitation bands corresponding to the charge transfer processes F− → Sm3+, O2− → Sm3+, and O2− → Ce3+, and the energy transfer processes Ce3+ → Sm3+ and O2− → Sm3+, were observed. The energies of the Sm3+ charge transfer transitions and the crystal field split Ce3+ 4f 05d 1 transitions were used to construct a complete host referred binding energy diagram for the series of lanthanide-doped NaMgF3:Ln compounds. We demonstrate that the optical and luminescence properties predicted by the binding energy diagram are in good agreement with those predicted by the binding energy diagram constructed via the alternative impurity-informed method, and all available experimental data regarding the NaMgF3:Ln compounds. We demonstrate that NaMgF3:Ln compounds are model systems for the study of charge trapping phenomena and divalent lanthanide luminescence. Ultimately, we validate that the impurity-informed method can be used to establish the energy levels of lanthanides in fluoride systems.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2022-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48439118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-11DOI: 10.1088/2050-6120/ac6ecb
Ádám Golcs, Korinna Kovács, Panna Vezse, L. Bezúr, P. Huszthy, T. Tóth
We report here the development of a surface-modified quartz glass sheet, which affords an opportunity for converting conventional spectrofluorometers to ion-selective optochemical sensors by placing it diagonally into a photometric cuvette. Moreover, we describe a generalizable technique, which allows the usage of any polymerizable ionophores for developing multiple-use fluorescent chemosensors of various selectivity. A fluorescent bis(acridino)-crown ether containing allyl groups was photocatalytically copolymerized with a methacrylate-acrylamide-based monomer mixture to obtain an ion-selective sensor membrane layer on the surface of the cuvette-compatible glass sheet. This glass membrane-based direct optode enabled the analysis of Zn2+ above a lower limit of detection of 2.2 × 10–7 mol·l−1 with an excellent reusability. Limiting factors, like pH and competing ionic or organic agents were thoroughly investigated. Moreover, spiked river-water samples were measured to demonstrate applicability. The proposed sensor placed in any conventional spectrofluorometer provides an innovative method for perturbation-free analysis of Zn2+ for all the chemists in need of a fast, easy-to-use, portable and regenerable analyzer without the requirement of an analyte-specific instrumentation.
{"title":"A cuvette-compatible Zn2+ sensing tool for conventional spectrofluorometers prepared by copolymerization of macrocyclic fluoroionophores on quartz glass surface","authors":"Ádám Golcs, Korinna Kovács, Panna Vezse, L. Bezúr, P. Huszthy, T. Tóth","doi":"10.1088/2050-6120/ac6ecb","DOIUrl":"https://doi.org/10.1088/2050-6120/ac6ecb","url":null,"abstract":"We report here the development of a surface-modified quartz glass sheet, which affords an opportunity for converting conventional spectrofluorometers to ion-selective optochemical sensors by placing it diagonally into a photometric cuvette. Moreover, we describe a generalizable technique, which allows the usage of any polymerizable ionophores for developing multiple-use fluorescent chemosensors of various selectivity. A fluorescent bis(acridino)-crown ether containing allyl groups was photocatalytically copolymerized with a methacrylate-acrylamide-based monomer mixture to obtain an ion-selective sensor membrane layer on the surface of the cuvette-compatible glass sheet. This glass membrane-based direct optode enabled the analysis of Zn2+ above a lower limit of detection of 2.2 × 10–7 mol·l−1 with an excellent reusability. Limiting factors, like pH and competing ionic or organic agents were thoroughly investigated. Moreover, spiked river-water samples were measured to demonstrate applicability. The proposed sensor placed in any conventional spectrofluorometer provides an innovative method for perturbation-free analysis of Zn2+ for all the chemists in need of a fast, easy-to-use, portable and regenerable analyzer without the requirement of an analyte-specific instrumentation.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2022-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48692897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-11DOI: 10.1088/2050-6120/ac6eca
R. Shanmugapriya, P. S. Kumar, C. Nandhini, K. Satheeshkumar, K. Vennila, K. Elango
A highly selective and sensitive assay of Al(III) using ratiometric fluorescence enhancement is reported in an aqueous solution. The probe (named RS5) exhibits a red-shift of 54 nm upon binding with Al(III) ion. The significant enhancement response of RS5 at 481 nm is attributed to the formation of a 1:1 complex between the probe and Al(III), wherein RS5 acts as a tridentate NNN-donor ligand. The complexation process is ascertained by 1H, 13C, and 27Al NMR and HR-MS spectral techniques. The binding constant of the complex is determined to be 1.3 × 105 M−1. The ratiometric change in fluorescence upon complexation with Al(III) is ascribed to an increase in intramolecular charge transfer (ICT) transition along with chelation enhanced fluorescence (CHEF) processes. The probe can be applied for monitoring Al(III) in a pH range of 6–8. The limit of detection (LOD) of RS5 for the examination of Al(III) is found to be 0.3 μM. With an aim to understand the sensing behavior of RS5, the optical properties of the probe and its Al(III) complex are investigated using density functional theory (DFT) and time-dependent density functional theory (TD-DFT) methods. The probe is successfully employed for the determination of Al(III), with very high recovery percentages, in natural matrices like deep well water, tap water, drinking water, pond water, river water, bovine serum albumin (BSA) solution and blood serum.
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