Mitochondrial DNA. Part B, Resources最新文献

Solanum carolinense Linnaeus, belonging to the family Solanaceae, is a perennial herb or subshrub. S. carolinense has become naturalized in Korea as an invasive species, forming a stable population that has grown naturally with native plants for more than 10 years. However, its chloroplast genome structure and complete sequence have not yet been reported. Therefore, we determined the complete chloroplast genome sequence of S. carolinense using genome sequencing, assembly, and annotation. The total length of the chloroplast genome was 155,315 bp with a GC content of 37.6%. It featured a quadripartite structure (a large single-copy region, 86,160 bp; a small single-copy region, 18,459 bp; and two inverted repeat regions, 25,348 bp each). It contains 129 genes, including 84 coding sequences (CDSs), 37 tRNA genes, 8 rRNA genes, and one pseudogene. Phylogenetic analysis of 78 CDSs revealed that S. carolinense is closely related to S. aridum Morong and S. hieronymi Kuntze. These results provide a molecular foundation for phylogenetic and evolutionary studies of the genus Solanum and present a fundamental chloroplast genomic resource for future invasion biology research.

This study reports the first complete mitochondrial genome (mitogenome) of Streblote castanea (Lepidoptera: Lasiocampidae), a keystone pest of tropical mangrove ecosystems. The mitogenome, spanning 15,400 bp, comprises 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two rRNA genes, and an A + T-rich control region, with a total AT content of 78.6%. This assembly addresses the critical genomic data gap for Streblote (Hübner, 1820) and Lasiocampidae, providing foundational insights for taxonomic revision, population dynamics tracing, and targeted biocontrol strategies in ecologically fragile mangrove habitats.

Guilandina minax (Hance) G. P. Lewis, commonly known as 'Whiteflower Cacalia', is a traditional medicinal plant extensively utilized by the Zhuang, Yao, and Dai ethnic minority groups. In this study, we sequenced and characterized the complete chloroplast genome of G. minax, and conducted a phylogenetic analysis to elucidate its evolutionary relationships within the Fabaceae. The chloroplast genome of G. minax was found to be 156,769 bp in length, with an overall GC content of 37.20%. It exhibited the typical quadripartite structure comprising a large single-copy (LSC) region of 85,264 bp, a small single-copy (SSC) region of 15,543 bp, and a pair of inverted repeat (IR) regions, each measuring 27,981 bp. A total of 127 genes were annotated from the chloroplast genome of G. minax, including 82 protein-coding genes, 37 transfer RNA (tRNA) genes, and 8 ribosomal RNA (rRNA) genes. A maximum-likelihood (ML) phylogenetic analysis based on the complete chloroplast genomes of G. minax along with 28 species from the Fabaceae family and 2 outgroup taxa indicated that G. minax is most closely related to Guilandina bonduc. This study provides valuable genomic resources for G. minax and contributes to a more resolved understanding of phylogenetic relationships within the Fabaceae.

Corydalis repens Mandl & Muhldorf is an early spring-blooming species within the genus Corydalis. In this study, we present the complete chloroplast genome of C. repens for the first time. The assembled genome is 188,101 base pairs (bp) long and is organized into a typical quadripartite structure consisting of a large single-copy (LSC) region of 89,844 bp, a small single-copy (SSC) region of 757 bp, and two inverted repeat (IR) regions totaling 48,750 bp. The overall GC content is 40.2%. The plastome encodes 141 genes, including 94 protein-coding genes, 38 transfer RNA genes, and eight ribosomal RNA genes, along with a single pseudogene (clpP). Phylogenetic reconstruction indicates that C. repens is nested within section Corydalis and shows the closest affinity to C. maculata. These findings provide an important genetic resource for future studies on the evolutionary history and conservation of the genus Corydalis.

The scyphozoan jellyfish Chrysaora chinensis has been frequently observed in the coastal waters of Fangchenggang, China, in recent years. This study assembled and analyzed the first complete mitochondrial genome (mitogenome) of C. chinensis from next-generation sequencing data. The mitogenome was 16,783 bp in length, exhibited an overall AT content of 69%, and encoded 13 typical protein-coding genes (PCGs), 2 ribosomal RNA (rRNA) genes (rrnS and rrnL), 2 transfer RNA (tRNA) genes (trnW-tca and trnM-cat), and an additional open reading frame (ORF), dpo. The mitochondrial gene order was largely consistent with that of previously reported jellyfish species. Furthermore, phylogenetic analysis of 13 mitochondrial PCGs from 19 jellyfish species indicated that Chrysaora chinensis is closely related to Chrysaora pacifica and Chrysaora quinquecirrha. These results provided a valuable reference for clarifying the taxonomy, evolutionary relationships, and phylogeography of scyphozoan jellyfish.

Fusicolla acetilerea (Nectriaceae, Ascomycota) is an emerging saprotrophic/plant-associated fungus whose genomic resources remain scarce. Here, we report the complete mitochondrial genome of F. acetilerea strain NIBRFGC000505922, assembled from Illumina NovaSeq reads. The circular-mapping mitogenome is 29,182 bp in length with a GC content of 28.7%. It encodes the canonical set of oxidative phosphorylation genes, two rRNA genes, 25 tRNA genes, and an intronic rps3 gene. A phylogenetic analysis based on 14 core protein-coding genes places F. acetilerea within Nectriaceae, forming a well-supported clade with Nectria cinnabarina. This mitogenome provides a reference for various genetic studies in Fusicolla and related taxa.

Gloiopeltis furcata is an annual red alga of Endocladiaceae, Florideophyceae, showing high economic and medical values. The chloroplast genome is instrumental for evolutionary studies, yet that of G. furcata remains unreported. Here, the chloroplast genome of G. furcata was assembled and analyzed. The genome size is 177,606 bp, which contains 184 genes (152 protein-coding genes, 29 tRNAs, and 3 rRNAs).  Four simple sequence repeat (SSR) types were detected. The AGA-encoded arginine had the highest relative synonymous codon usage (RSCU) value, while CGG-encoded arginine had the lowest. Phylogenetic analysis indicated that G. furcata is a sister to Chondrus crispus.

Camellia obtusifolia is a wild relative of the economically important oil crop Camellia oleifera. Here, we sequenced and analyzed the complete chloroplast genome of C. obtusifolia to clarify its phylogenetic position. The cp genome was 156,540 bp in length and exhibited a typical quadripartite structure comprising a LSC (86,254 bp), a SSC (18,400 bp), and two IR (25,943 bp) regions. It contained 134 genes, including 88 protein-coding genes, 37 tRNA genes, and eight rRNA genes, with a GC content of 37.3%. Comparative analysis revealed a 452-bp deletion in the atpH-atpI intergenic region, which served as a distinguishing feature between C. obtusifolia and C. oleifera. Phylogenetic analysis showed that C. obtusifolia clustered into a clade with Camellia sasanqua, Camellia brevistyla, and Camellia meiocarpa, demonstrating the closest relationship with C. sasanqua (bootstrap = 98%), contradicting previous morphological classifications. This study provided essential genomic resources for future phylogenetic and conservation studies in genus Camellia.

This study presents the complete mitogenome sequence of Atheraster arandae, providing valuable information for its genetic and taxonomic research. Through next-generation sequencing, we successfully obtained the complete mitogenome of A. arandae, with a length of 16,292 bp. The mitogenome consisted of 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, and 2 ribosomal RNA (rRNA) genes. Both Maximum Likelihood and Bayesian inference phylogenetic trees demonstrated that A. arandae clustered closely with Iconaster longimanus. By revealing its mitogenome and positioning its phylogenetic placement within the Goniasteridae lineage, this study provides insights into the phylogenetic context of A. arandae.

Zingiber ottensii Valeton (1918) is a perennial herb in Zingiberaceae, which has important medicinal and edible values. Here, we first reported and characterized the complete chloroplast genome (plastome) of Z. ottensii using the whole-genome resequencing technology. The plastome was 169,569 bp in size and contained 138 genes, including 92 protein-coding genes, 38 tRNA genes, and 8 rRNA genes. It exhibits a typical quadripartite structure with a large single-copy region (LSC, 86,760 bp), a small single-copy region (SSC, 7,615 bp), and a pair of inverted repeats (IRs, 37,597 bp). Phylogenetic analysis confirmed that Z. ottensii belonged to Zingiber sect. Zingiber and revealed its close relationship to Zingiber zerumbet (L.) Roscoe ex Sm. These results provided essential genomic resources for future phylogenetic and evolutionary studies of Zingiber Mill.