Mitochondrial DNA. Part B, Resources最新文献

Heterojapygidae is a relict and poorly studied family of Diplura that holds significant importance for understanding dipluran evolutionary history. It has the fewest described species among the 10 dipluran families. This study presents the complete mitochondrial genome of Heterojapyx souliei Bouvier, 1905, the largest dipluran species known to date, from the Tibetan Plateau. This represents the first report of a complete mitochondrial genome from Heterojapygidae. The circular mitochondrial genome is 15,146 bp in length and encodes the 37 typical mitochondrial genes, including 13 protein-coding genes (PCGs), 22 tRNA genes, and 2 rRNA genes, arranged in the same order as in other diplurans. The base composition of the majority strand is 35.26% for A, 28.06% for C, 15.76% for G, and 20.92% for T. Phylogenetic trees inferred from 13 PCGs using the maximum-likelihood (ML) methods show that Heterojapygidae is a sister to Japygidae + Evalljapygidae, suggesting that the divergence of Heterojapygidae occurred later than Parajapygidae, but earlier than Japygidae and Evalljapygidae. Our study fills a critical gap and provides new insights into the phylogeny of Diplura.

Aesculus pavia L., a member of the genus Aesculus in the family Sapindaceae, holds significant value as both a medicinal and ornamental plant. In this study, we assembled and annotated the complete chloroplast genome of A. pavia and conducted the phylogenetic analysis among the genus Aesculus. The complete chloroplast genome of A. pavia is 156,394 bp in length, with a GC content of 37.90%. It exhibits a typical quadripartite structure, consisting of a large single-copy (LSC) region (85,927 bp), a small single-copy (SSC) region (18,751 bp), and a pair of inverted repeats (IRs) regions (25,858 bp). A total of 133 genes were annotated, including 88 protein-coding genes (PCG), 37 tRNA genes, and 8 rRNA genes. Phylogenetic analyses revealed that A. pavia is clustered with A. turbinata and A. hippocastanum, suggesting a close relationship between A. pavia and the two species. This study provides a new plastome sequence for evolutionary and phylogenetic studies of the genus Aesculus.

In this study, the complete mitochondrial genome of Leucoptera malifoliella (Costa, 1836) (Lepidoptera: Lyonetiidae) was sequenced and annotated. The genome is 15,214 bp in size, comprises 37 typical genes and a control region, and shows a positive AT skew. The gene content and order are similar to those of other lepidopterans. Phylogenetic analysis confirmed that families within Yponomeutoidea are monophyletic. The relationships among families are inferred as (Praydidae + Attevidae) + (Lyonetiidae + (Scythropiidae + Plutellidae)). This research improves our understanding of the relationships among major Yponomeutoidea lineages.

Polygala chinensis L. is a medicinal herb with anti-inflammatory activity and traditional use against snake envenomation. We assembled and annotated its complete chloroplast genome using Illumina data. The chloroplast genome is 163,097 bp and exhibits a typical quadripartite structure, comprising a large single-copy region (83,285 bp), two inverted repeats (34,925 bp each), and a small single-copy region (9,962 bp). It contains 113 unique genes, including 78 protein-coding genes, 30 tRNA genes, and 5 rRNA genes. Phylogenetic analysis placed P. chinensis as sister to Polygala subopposita. This work provides the first species-specific chloroplast genome reference for P. chinensis, enabling precise molecular identification and evolutionary studies.

Bergenia crassifolia (L.) Fritsch. is a traditional medicinal species. This study reports its complete chloroplast (cp) genome for the first time. This cp genome is a quadripartite structure with 156,378 base pairs (bp) long and its GC contain is 38%. It is comprised of 128 annotated genes, including 84 protein-coding genes, 36 tRNAs, and 8 rRNAs. Phylogenetic analysis revealed a sister relationship among B. crassifolia, B. purpurascens and B. scopulosa, and it formed a monophyletic group with other closely associated species. The genomic data and findings from the phylogenetic studies of B. crassifolia provide valuable insights for further research on the evolution patterns of this understudied species and Bergenia.

We have sequenced and annotated the complete mitochondrial genome of Carabus brandti (Coleoptera: Carabidae). This mitogenome is a total of 16,794 bp in length and encodes 13 protein-coding genes (PCGs), 22 transfer RNA genes, two ribosomal RNA unit genes (12S and 16S), and one non-coding control region (CR). The Bayesian inference phylogenetic analysis confirmed the taxonomic status of C. brandti, showing its close relationship with C. smaragdinus and C. granulatus. This study provides the first comprehensive characterization of the mitochondrial genome of C. brandti, providing valuable insights for phylogenetic systematic studies within the subfamily Carabinae.

In this study, we conducted a comprehensive sequencing and characterization of the chloroplast genome of the Asian endemic species Calystegia pubescens 'Anestia'. The complete genome is 152,079 bp long, with a GC content of 37.37%. It consists of a large single-copy region (87,891 bp), a small single-copy region (19,888 bp), and a pair of inverted repeats regions (22,149 bp). A total of 126 genes were identified, including 81 protein-coding genes, 37 tRNA genes, and eight rRNA genes. Phylogenetic analysis revealed a close relationship between C. pubescens 'Anestia' and C. hederacea. These results provide valuable genetic insights for species identification and phylogenetic studies within the genus Calystegia.

The complete mitochondrial genome of Rasbora atridorsalis Kottelat and Chu (Spixiana. 10(3):313-318) is first characterized herein (GenBank accession: OM617726.1). The circular mitogenome is 16,524 base pairs (bp) in length, containing 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and two non-coding regions, with an AT bias of 58.7%. A Bayesian phylogenetic reconstruction, based on the amino acid sequences of 13 mitochondrial protein-coding genes from R. atridorsalis and 41 congeneric species, resolved the evolutionary relationships within the genus, providing molecular evidence for intra-generic affinities. This work fills a critical genomic gap in Rasbora phylogenetics, provides a molecular basis for taxonomic revisions, and enhances understanding of the evolutionary history and interspecific relationships in Rasborinae.

Anemone sylvestris Linnaeus (1753) is a species in the Ranunculaceae family within the genus Anemone. It is mainly distributed in China, Croatia, and Poland. The chloroplast genome has a total length of 161,033 bp, comprising a large single-copy region of 81,144 bp, a small single-copy region of 17,393 bp, and two inverted repeat regions of 31,248 bp each. The GC content is 37.54%. A total of 134 genes were annotated, including 90 protein-coding genes, 8 rRNA genes, and 36 tRNA genes. Phylogenetic analysis using the maximum likelihood method robustly supported the close relationship of A. sylvestris with other Anemone species, namely A. raddeana, A. reflexa, and A. altaica, forming a well-defined monophyletic clade. The complete chloroplast genome of A. sylvestris reported here provides a useful resource for subsequent phylogenetic, identification, and resource related studies.

Globba racemosa, a Zingiberaceae species native to southern and southwestern China, the complete chloroplast genome of G. racemosa was sequenced and reported for the first time. The genome was 163,321 bp in total length, possess typical quadripartite structures that consist of a large single copy (LSC, 88,111 bp), a small single copy (SSC, 15,770 bp) and a pair of inverted repeats (IRs, 29,720 bp). The complete chloroplast genome contained 134 genes (87 protein-coding, 39 tRNA, 8 rRNA), with an overall GC content of 35.8%. phylogenetic analysis based on 10 chloroplast genomes indicated its close affinity to R. multiflora in Glolla. This newly reported chloroplast genome can provide fundamental genetic data for future research on the genus Globba.