Mitochondrial DNA. Part B, Resources最新文献

The Mycenaceae family comprises one of the most taxonomically diverse and ecologically important groups of saprotrophic fungi, yet no mitochondrial genome (mitogenome) has been reported to date. This study reports the first mitogenome of Mycenaceae, sequenced from Mycena pura (Pers.) P. Kumm. 1871. The 107,684 bp circular mitogenome (GC 27.83%) contains 14 conserved protein-coding genes (PCGs), 25 tRNAs, 2 rRNAs, and 11 non-conserved open reading frames (ORFs), with 18 introns (mostly harboring ORFs) identified in seven genes. Phylogenetic analysis based on mitochondrial amino acid sequences validated its Mycenaceae placement, providing crucial genomic resources for future evolutionary studies.

The mitochondrial genome of Grapsus adscensionis (Osbeck, 1765), a rocky shore crab distributed across the Macaronesian archipelagos and the eastern Atlantic, was sequenced and annotated for the first time. The circular mitogenome is 15,553 bp in length and comprises 13 protein-coding genes (PCGs), 22 tRNAs, two rRNAs, and a non-coding control region. It exhibits a strong AT bias, with negative AT- and GC-skews. Gene arrangement and composition are consistent with other brachyuran crabs. These findings provide novel molecular data for the species, representing the third mitogenome published for the genus Grapsus, and contribute to a better understanding of brachyuran evolutionary relationships.

The silver-backed chevrotain (Tragulus versicolor Thomas, 1910) is the only endemic ungulate species in Vietnam and among the most little-known ungulates in the world. The species currently has a very narrow distribution area and is facing many threats of extinction due to habitat loss and illegal hunting. In this study, we describe complete mitochondrial genome of T. versicolor and its taxonomic position within Tragulidae. The circular genome is 16,317 bp in length, comprising 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and one control region, with a GC content of 40.3%. Phylogenetic analysis based on complete mitochondrial genomes using the maximum-likelihood method confirms the monophyly of Tragulidae and the distinct position of T. versicolor within Tragulus. This is the first publicly available mitogenome of T. versicolor (GenBank accession no. PV872897), which provides an essential resource for future studies on evolution, taxonomy, and conservation of this elusive species.

Zehneria japonica (Thunb.) H. Y. Liu is a traditional Chinese medicinal plant. We sequenced and annotated its complete chloroplast genome, which is 157,052 bp in length with 36.99% GC content. It exhibits a typical quadripartite structure, including a large single-copy region of 86,849 bp, a small single-copy region of 17,867 bp, and two inverted repeat regions of 26,168 bp. The genome encodes 131 genes (86 protein-coding, 37 tRNA, and 8 rRNA). Phylogenetic analysis based on chloroplast genomes supports the monophyly of Zehneria species. These results enrich genomic resources and provide a foundation for future phylogenetic and evolutionary studies within Zehneria.

Bibio Geoffroy, 1762 is a species-rich genus within the family Bibionidae. In this study, we present the complete mitochondrial genome of Bibio hordeiphagus Yang and Luo 1988 for the first time. Complete mitochondrial genome of B. hordeiphagus is 15,496 bp in length, comprising 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and a control region. The nucleotide composition of mitochondrial genome of B. hordeiphagus was 40.55% of A, 39.02% of T, 8.37% of G, and 12.05% of C. Based on the sequencing and annotating results, we conducted a preliminary phylogenetic analysis of Bibionomorpha. Our findings support the monophyly of both family Bibionidae and genus Bibio, with the family Bibionidae identified as the sister group to Sciaridae and Mycetophilidae. This study encompasses all reliable annotated mitochondrial genome data for Bibionidae and analyzed the higher-level phylogenetic relationships within the Bibionomorpha from the perspective of mitochondrial genome data.

Persicaria hydropiper (L.) Spach and P. pubescens (Blume) Hara (Polygonaceae) are widely used as traditional Chinese medicinal plants. In this study, the complete chloroplast (cp) genomes of P. hydropiper and P. pubescens were sequenced, assembled, and annotated. The cp genome sizes of P. hydropiper and P. pubescens are 159,054 bp and 159,502 bp, respectively. The cp genomes of P. hydropiper and P. pubescens each contain a total of 130 genes, including 86 protein-coding, 37 tRNA, and 8 rRNA genes. Phylogenetic analysis reveals that P. hydropiper is close to P. pubescens and P. japonica, and Persicaria is mostly closely related to Bistorta.

Amynthas susakii (Kobayashi 1936) is an earthworm species endemic to Korea that typically inhabits mountainous forest environments. The specimens were collected from Mount Jiri, South Korea. The complete mitogenome of A. susakii was sequenced, assembled, and annotated. The mitogenome of A. susakii is a circular DNA molecule of 15,115 bp with an A + T content of 67.7%, comprising 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and 1 control region. Phylogenetic analysis showed that A. susakii clustered with other Amynthas species in the well-supported Megascolecidae family.

This study performed mitochondrial genome sequencing on Minettia omei, which belongs to Lauxaniinae, a subfamily of Lauxaniidae. The complete mitochondrial genome spans 16,201 bp and comprises 37 genes, including 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a control region. Base composition: A 38.81%, T 38.81%, C 12.93%, G 9.44% , A + T bias 77.62%. COI uses start codon CAA; COI, COII, NAD2, NAD1, NAD4 have incomplete T + tRNA stop codons. Phylogenetic analysis elucidates the species' evolutionary relationships, establishing a critical genetic foundation for future studies on Lauxaniidae.

Tephrinectes sinensis, an endemic flatfish from China's coastal waters, has remained taxonomically contentious owing to its distinctive morphology and osteology. We sequenced and annotated the first complete mitochondrial genome of this species using Illumina HiSeq 2500. The circular mitogenome spans 17,366 bp, encoding 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and a control region. A maximum-likelihood phylogeny places T. sinensis as a single-taxon lineage sister to the remaining Paralichthyidae. This matrilineal pattern is congruent with the long-standing morphological debate over its familial placement.The complete mitogenome provides a foundational genomic resource for resolving relationships within Pleuronectiformes.

Euphorbia neriifolia L. is a medicinal and ornamental species widely distributed in India and China, yet its complete chloroplast genome has not been reported. Here, we present its first complete plastome (164,435 bp; GC: 34.97%), featuring a quadripartite structure with a large single-copy (92,653 bp), a small single-copy (18,282 bp), and two inverted repeat (26,750 bp each) regions. The genome encodes 124 genes, including 79 protein-coding genes, 37 tRNAs, and eight rRNAs. Phylogenetic analysis revealed a close relationship with E. royleana. This genome provides a fundamental resource for phylogenetic studies and further genetic research in Euphorbia.