Mitochondrial DNA. Part B, Resources最新文献

Rhabdophis chiwen is currently found so far in Sichuan Province, China, where it predominantly feeds on earthworms and firefly larvae. In this study, we sequenced and analyzed the mitochondrial genome of R. chiwen, which measured 17,646 bp in length and encompassed 37 genes along with two control regions. The base composition revealed percentages of 33.20% A, 25.94% T, 13.27% G, and 27.59% C. Phylogenetic analyses indicate that R. chiwen belongs to the family Colubridae and forms a sister branch with R. tigrinus. This study successfully obtained the first complete mitochondrial genome of R. chiwen, offering crucial genetic data for its evolutionary history and conservation.

Periclimenes brevicarpalis is widely distributed in the Indo West Pacific oceans. The mitochondrial genome of P. brevicarpalis was sequenced and assembled firstly by next generation sequencing technology in our study. The complete mitochondrial genome of P. brevicarpalis was 16,673 bp in size, consisted of 22 transfer RNA genes, 13 protein coding genes and two ribosomal RNA genes. The contents of the four bases were C (25.62%), T (28.73%), A (31.64%), and G (14.01%). The result of phylogenetic analysis showed that P. brevicarpaliswas clustered with Anchistus australis. In conclusion, our research provided valuable data for phylogenetic analysis of the Palaemonidae family as the first report about mitochondrial genomes in Periclimenes.

Liparis gigantea is a Chinese traditional medicinal herb in the Orchidaceae family. It is a rare and special Liparis species that exhibits relatively large flowers. To illuminate its phylogenetic status and augment genomic resources, the complete chloroplast (cp) genome of L. gigantea was first sequenced and assembled using whole genome next-generation sequencing in this study. The cp genome size is 158,462 bp with a total GC content of 36.9%. Characterized by a quadripartite structure, the genome consists of a large single-copy (LSC) region of 86,032 bp, a small single-copy (SSC) region of 18,322 bp, which is separated by a pair of 27,054 bp inverted repeat regions (IRs). A total of 133 genes were annotated, including 87 protein-coding genes, 38 tRNA genes, and 8 rRNA genes. Phylogenetic analysis strongly supported L. gigantea as the sister to two closely related terrestrial species, Liparis nervosa and L. vivipara. The results of this study provide genomic information for future research and application of this medicinal herb.

Primula medogensis W.B Ju, B. Xu & X.F. Gao 2023, a new species categorized under P. sect. Cordifoliae, was officially described in 2023. Given its recent classification, the genetic resources for this species are currently very limited. Here, we sequenced and assembled the first complete chloroplast genome of P. medogensis using Illumina sequencing technology. The complete chloroplast genome of P. medogensis is 151,486 bp in length, exhibiting a typical quadripartite structure. It consists of a large single-copy region (LSC; 83,407 bp) and a small single-copy region (SSC;17675 bp), separated by a pair of inverted repeat regions (IRs; 25202 bp). A total of 131 genes were annotated, including 86 protein-coding, 37 tRNA, and eight rRNA genes. The overall GC content was 37.1%. Phylogenetic analysis of 59 Primula species revealed a close relationship between P. medogensis and P. calliantha subsp. bryophila.

Artemisia sylvatica Maximowicz 1859 is one of the medicinal herbs in Artemisia. This study presents the complete chloroplast genome of A. sylvatica, sequenced using the Illumina NovaSeq platform. The genome is 151,161 bp in length, featuring a GC content of 38%. It consists of a large single-copy (LSC) region of 82,892 bp, a small single-copy (SSC) region of 18,353 bp, and two inverted repeat (IR) regions of 24,958 bp each. In total, the genome contains 132 genes, including 87 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Phylogenetic analysis positions A. sylvatica within the subgenus Artemisia, highlighting its evolutionary relationships within this diverse genus. The first chloroplast genome of A. sylvatica was reported in this work contributes to the enrichment of genomic data for the genus Artemisia.

This study presented the first sequencing and analysis of the mitochondrial genome of Paraclius curvispinus Yang and Saigusa, 2001, a predator fly belonging to the family Dolichopodidae (long-legged fly). The complete mitochondrial genome of P. curvispinus was 16,882 bp long (GenBank accession number: PP498802), which contained 13 protein-coding genes, 22 tRNAs, 2 rRNAs, and a control region. The nucleotide composition of mitochondrial genome of P. curvispinus was 38.48% of A, 36.00% of T, 10.67% of G, and 14.85% of C. Maximum-likelihood (ML) analysis revealed the phylogenetic relationships within the subfamily Dolichopodinae, suggesting the following grouping: ((Dolichopus + Lichtwardtia)+((Hercostomus + Poecilobothrus)+ Gymnopternus))+ Paraclius. This study provides a basis for discussing the evolution of mosquito larval predation habits of Dolichopodinae.

Arytera littoralis Blume 1847 is an evergreen small tree belonging to the Sapindaceae family. It is distributed in South China to SE Asia and the Solomon Islands. However, the chloroplast genome of A. littoralis has yet to be reported. In this study, the complete chloroplast genome of Arytera littoralis was determined. The total genome size was 161,091 bp in length, consisting of two inverted repeats (IRs) (28,432 bp) separated by the large single-copy (LSC) (85,737 bp) and small single-copy (SSC) (18,490 bp) regions. The genome contained 133 genes, including 87 protein-coding genes, 37 tRNA genes, and eight rRNA genes. The GC content of the complete chloroplast genome was 37.8%. A maximum-likelihood (ML) phylogenetic tree of A. littoralis and 13 related species from the family Sapindaceae indicated that A. littoralis was close to a clade composed of Sapindus, Nephelium, Litchi, and Dimocarpus. This study will offer essential genetic resources of A. littoralis and provide insights into the phylogeny and evolution of Sapindaceae.

Garcinia esculenta Y. H. Li. 1981 (Clusiaceae) is an endemic tree species in China, primarily found in western and northwestern Yunnan Province. In this research, the complete chloroplast genome of G. esculenta was sequenced using the Illumina NovaSeq6000 platform. The result showed that the length of the complete chloroplast genome was 155,853 bp, which was composed of a large single-copy region (LSC) of 84,534 bp, a small single-copy region (SSC) of 17,175 bp, and a pair of inverted repeat (IR) regions of 27,072 bp. The overall GC content was 36.1%. The complete chloroplast genome encompassed 128 genes, comprising 83 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Phylogenetic analysis of the complete chloroplast genome sequences of 22 species revealed that G. esculenta is most closely related to G. oblongifolia.

Hordeum pusillum (Hordeum pusillum Nutt., 1818) is an annual barley that is native to the west of the North America and widespread in southern United States and tropical America. In this study, we have provided the first complete characterization of the chloroplast genome of H. pusillum. Our research revealed that the circular chloroplast genome of H. pusillum consists of a large single-copy region (LSC: 80,853 bp), a small single-copy region (SSC: 12,745 bp), and a pair of inverted repeat regions (IRs: 21,587 bp), totaling 136,772 bp in length. Within the chloroplast genome of H. pusillum, 91 protein-coding genes, 38 tRNA genes, and ten rRNA genes were identified. To determine the evolutionary relationship of Hordeum species with reported chloroplast genome sequences, we constructed a phylogenetic tree using the entire chloroplast genome sequences. The evolutionary position of H. pusillum corresponds to its geographical location. The chloroplast genome of H. pusillum provided in this study may have significant implications for the phylogenetic study of Poaceae species.

Baeckea frutescens Linaeus 1753, as a traditional folk medicine in South East Asia, possesses sesquiterpenes, phloroglucinols, chromones, and essential oil, and is utilized for traditional Chinese medicinal purposes. The genetic diversity of the plant must be better understood, considering its significance. The complete chloroplast (cp) genome of B. frutescens was sequenced and assembled by using Illumina paired-end data, marking a significant advancement toward comprehending its genetic composition. The complete cp genome is 158,939 bp in length and contains 128 genes, consisting of 83 protein-coding genes, 8 ribosomal RNA genes, and 37 transfer RNA genes. Phylogenetic analyses indicated that B. frutescens and other the 13 were clustered to the family of Myrtaceae. These findings are crucial for the conservation and utilization of this important plant species. Additionally, they underscore the potential for future research on the evolution and preservation of B. frutescens, which could be advantageous in pharmaceutical applications.