Mitochondrial DNA. Part B, Resources最新文献

Protea cynaroides 'Red Rex' (James Petiver 1698) is a perennial evergreen shrub of the Proteaceae family valued for ornamental and medicinal uses, yet its genomic data remain unexplored. Here, we report the complete chloroplast genome sequence (157,894 bp) of P. cynaroides 'Red Rex'. The genome exhibits a quadripartite structure, including a large single-copy region (86,427 bp), a short single-copy region (18,553 bp), and two inverted repeats (26,457 bp each), with 38.06% GC content. A total of 130 genes were annotated. Phylogenetic analysis confirmed close relationships within Proteaceae, advancing insights into the genetics and evolution of Protea.

Luciogobius parvulus (Snyder, 1909) is distributed in restricted areas of Japan and South Korea. Here, we report the first complete mitochondrial genome of this species. The 16,488 bp mitogenome contains 13 protein-coding genes, 22 tRNAs, and two rRNAs, with no gene rearrangements detected. Four protein-coding genes (COX2, COX3, ND4, and CytB) exhibit incomplete stop codons. Maximum-likelihood analysis of Gobionellinae indicates that two Luciogobius species may require morphological reexamination. Only five of the 17 Luciogobius species currently have complete mitogenomes. These results provide essential genomic data for future phylogenetic and taxonomic studies.

Polylepis rugulosa Bitter is an endangered species native to the central and northern Andes and is one of the woody species that form the highest altitude woodlands in the world. Despite its ecological importance, genomic information for P. rugulosa is scarce, which limits our understanding of its evolutionary relationships and hinders the development of conservation strategies. In this study, we assembled the complete chloroplast genome of P. rugulosa and conducted phylogenetic analyses using 25 chloroplast genomes. The chloroplast genome is composed of 155,163 bp, with a typical quadripartite structure: a large single copy (85,290 bp), a small single copy (18,761 bp), and two inverted repeats (26,556 bp each). A total of 129 genes were identified, including 84 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Phylogenetic analysis confirmed the placement of P. rugulosa within the Sanguisorbinae clade (Sanguisorbeae tribe). This is the first report of the complete plastome genome of a Polylepis species, providing valuable genomic data for conservation strategies and for reconstructing the biogeographical history of the genus Polylepis in South America.

The complete mitochondrial genome (mitogenome) of the symmetrical brain coral Pseudodiploria strigosa (Dana, 1846) is sequenced for the first time in this study. The newly assembled mitogenome is 16,906 bp long and encodes 13 protein coding genes (PCGs), 2 tRNA genes (trnM[cat] and trnW[tca]), and 2 ribosomal genes (rrnL and rrnS). A group I intron bisected the PCG nad5. The studied mitogenome also includes two relatively long non-coding regions, one 910 bp long, located between cox3 and cox2 and a second 799 b long, located between cox1 and trnM(cat). Mitochondrial synteny is identical to that previously reported in cofamilial corals. The mitogenome of P. strigosa is AT-rich with nucleotide use as follows: A = 24.84%, C = 13.21%, G = 20.41%, and T = 41.54%. A maximum-likelihood phylomitogenomic analysis that relied on the phylogenetic signal provided by translated PCGs supported the monophyly of the family Faviidae to which P. strigosa belongs. This newly assembled mitochondrial genome will be employed as a reference in studies biomonitoring this and other imperiled corals in the Caribbean basin using environmental DNA.

Bolbitis angustipinna (Hayata) H. Ito is a rare ornamental fern primarily distributed throughout East Asia and Southeast Asia. Its complete chloroplast genome was assembled, and a phylogenetic tree was constructed using 15 fern species. The genome is 152,562 base pairs, with a large single-copy region (82,712 bp), a small single-copy region (21,403 bp), and two inverted repeat regions (24,208 bp). It encodes 115 genes, including 72 protein-coding genes, 35 tRNA genes, and 8 rRNA genes. Phylogenetic analysis shows that B. angustipinna is most closely related to B. deltigera, providing valuable genetic and evolutionary insights.

Cerastoderma glaucum is a key intertidal bivalve in the north-east Atlantic, serving as a trophic resource for fish and birds. Its mitochondrial genome comprises 14,953 bp, encoding 13 protein-coding genes, 2 rRNA genes, and 22 tRNA genes. Gene structure resembles Cerastoderma edule but lacks an additional methionine tRNA. Phylogenetic analysis revealed close affinity between Baltic and English samples (p-distance = 0.027). No mitochondrial heteroplasmy was detected in mature male gonads, suggesting C. glaucum is not a doubly uniparental inheritance (DUI) species. These findings contribute to understanding mitogenomic variation and reproductive biology in bivalves inhabiting dynamic coastal ecosystems .

Coelophora saucia is an important natural enemy insect in agricultural production. We determined the complete mitochondrial genome of the C. saucia by high-throughput sequencing, and the mitogenome was 18,068 bp in length, with a GC content of 20.9%, encodes 2 ribosomal RNA genes, 22 transporter RNA genes, 13 protein-coding genes and with a non-coding control region. A phylogenetic tree was constructed using the maximum likelihood (ML) method, and the results indicated that the C. saucia was most closely related to Propylea japonica and Propylea quattuordecimpunctata.

The two Lamioideae species, Lamium barbatum Siebold & Zucc. 1846, is distributed across Europe, North Africa, Asia, and North America, while Leucas ciliata Hochst. ex Benth. 1829 is found in southern China and Southeast Asia. We sequenced their complete chloroplast genomes using the Illumina platform, obtaining lengths of 150,887 bp and 151,259 bp, respectively. Phylogenetic analysis showed Lamium barbatum is closely related to four other Lamium species, including Lamium takeshimense, Lamium album, Lamium amplexicaule, and Lamium galeobdolon. The Leucas ciliata species clusters with Leucas mollissima. These findings provide valuable data for species identification, evolutionary research, and conservation genetics.

Iris confusa Sealy is highly valued for its ornamental and medicinal applications. Here, we report the complete chloroplast genome sequence of I. confusa obtained using Illumina pair-end sequencing. The complete circular genome of I. confusa is 152,470 bp in length and contains a large single-copy region (83,251 bp), a small single-copy region (18,503 bp), and two short inverted repeat regions (25,358 bp). The genome sequence encodes 133 genes including 87 protein-coding genes, 8 ribosomal RNA genes, and 38 transfer RNA genes. This study will facilitate the understanding of chloroplast genome features of genus Iris and contribute to further studies on phylogenetic analyses.

Sorbaria sorbifolia var. glandulifolia, endemic to Korea, is distinguished by glandular trichomes on the abaxial leaflet surface. In this study, we present its first complete chloroplast genome (159,476 bp) with a typical quadripartite structure comprising 112 unique genes, including 78 protein-coding genes, 30 transfer RNAs, and four ribosomal RNAs. Phylogenetic analysis of 18 complete chloroplast genomes revealed that S. sorbifolia var. glandulifolia forms a monophyletic group with other Sorbaria species and clusters with Adenostoma. Furthermore, two well-supported clades within Sorbaria corresponded to the sections Sorbaria and Kirilowiana. This study provides the first chloroplast genome report for S. sorbifolia var. glandulifolia, offering valuable genomic insights for future phylogenetic and taxonomic research on the genus Sorbaria.