Mitochondrial DNA. Part B, Resources最新文献

Coelophora saucia is an important natural enemy insect in agricultural production. We determined the complete mitochondrial genome of the C. saucia by high-throughput sequencing, and the mitogenome was 18,068 bp in length, with a GC content of 20.9%, encodes 2 ribosomal RNA genes, 22 transporter RNA genes, 13 protein-coding genes and with a non-coding control region. A phylogenetic tree was constructed using the maximum likelihood (ML) method, and the results indicated that the C. saucia was most closely related to Propylea japonica and Propylea quattuordecimpunctata.

Iris confusa Sealy is highly valued for its ornamental and medicinal applications. Here, we report the complete chloroplast genome sequence of I. confusa obtained using Illumina pair-end sequencing. The complete circular genome of I. confusa is 152,470 bp in length and contains a large single-copy region (83,251 bp), a small single-copy region (18,503 bp), and two short inverted repeat regions (25,358 bp). The genome sequence encodes 133 genes including 87 protein-coding genes, 8 ribosomal RNA genes, and 38 transfer RNA genes. This study will facilitate the understanding of chloroplast genome features of genus Iris and contribute to further studies on phylogenetic analyses.

Sorbaria sorbifolia var. glandulifolia, endemic to Korea, is distinguished by glandular trichomes on the abaxial leaflet surface. In this study, we present its first complete chloroplast genome (159,476 bp) with a typical quadripartite structure comprising 112 unique genes, including 78 protein-coding genes, 30 transfer RNAs, and four ribosomal RNAs. Phylogenetic analysis of 18 complete chloroplast genomes revealed that S. sorbifolia var. glandulifolia forms a monophyletic group with other Sorbaria species and clusters with Adenostoma. Furthermore, two well-supported clades within Sorbaria corresponded to the sections Sorbaria and Kirilowiana. This study provides the first chloroplast genome report for S. sorbifolia var. glandulifolia, offering valuable genomic insights for future phylogenetic and taxonomic research on the genus Sorbaria.

Tinospora sinensis (Lour.) Merr. (Menispermaceae) is a medicinal plant with limited genomic resources available. Here, we assembled and annotated its 160,664 bp chloroplast genome, identifying 126 genes with a GC content of 39.31%. We detected 256 SSRs and 50 dispersed repeats, which represent potential molecular markers. IR boundary analysis showed slight expansions/contractions involving rps19, ndhF, and ycf1. Phylogenetic analysis confirmed its close evolutionary relationship with other Tinospora species. This is the first comprehensive genomic characterization of T. sinensis, providing valuable resources for taxonomy, evolution, and medicinal research in Menispermaceae.

The two Lamioideae species, Lamium barbatum Siebold & Zucc. 1846, is distributed across Europe, North Africa, Asia, and North America, while Leucas ciliata Hochst. ex Benth. 1829 is found in southern China and Southeast Asia. We sequenced their complete chloroplast genomes using the Illumina platform, obtaining lengths of 150,887 bp and 151,259 bp, respectively. Phylogenetic analysis showed Lamium barbatum is closely related to four other Lamium species, including Lamium takeshimense, Lamium album, Lamium amplexicaule, and Lamium galeobdolon. The Leucas ciliata species clusters with Leucas mollissima. These findings provide valuable data for species identification, evolutionary research, and conservation genetics.

Genomic data of the Eurasian eagle-owl (Bubo bubo Kiautschenis; B. bubo), an endangered species in South Korea is lacking. This study presents its first complete mitochondrial genome of Bubo bubo (GenBank accession no. OR756278). The resulting mitochondrial genome was 18,957 bp long and included two ribosomal RNAs, 13 protein-coding genes, 23 transfer RNAs, and two control regions, respectively. The mitogenome of B. bubo consisted of 29.6% A, 22.5% T, 33.8% C, 14.1% G, and 52.1% AT. Phylogenetic analysis revealed that B. bubo individuals were well grouped within the Strigidae.

This study describes the mitogenome of Ceratina smaragdula (Hymenoptera: Apidae), which consists of 15,591 base pairs with an AT content of 85.7%. It contains 37 genes, including 13 protein-coding genes, two rRNA genes, 22 tRNA genes, and a control region. Phylogenetic analysis of 40 Apidae insect mitogenomes shows that Xylocopinae and Nomadinae are monophyletic, while Apinae is paraphyletic. C. smaragdula is placed within Xylocopinae, forming a sister group with Ceratina okinawana. The genus Apis is closer to Lepidotrigona than to Melipona or Bombus.

Reeves' muntjac (Muntiacus reevesi Ogilby, 1839) is a small deer species native to China, primarily residing in the mountainous regions of the southeastern part of the country. In this study, the mitochondrial genome of a Reeves' muntjac specimen from Chongqing, southeastern China, was sequenced. The genome consists of 16,356 base pairs (GenBank: PP506676), including 13 protein-coding genes, 22 tRNA genes, two rRNA genes, OL, and control region. Phylogenetic analysis disclosed significant genetic differences between the Reeves' muntjac from Chongqing and those previously reported, indicating that its population may possess rich genetic diversity or there might be undiscovered, geographically isolated groups.

We report the complete mitochondrial genome of the Korean Clawed Salamander (Onychodactylus koreanus), endemic to the Korean Peninsula. The 16,445 bp mitogenome, assembled from Illumina HiSeq data, contains 37 genes (13 PCGs, 22 tRNAs and 2 rRNAs) plus the control region and has an A + T content of 68.6%. Phylogenetic analysis confirms it is a distinct lineage within Onychodactylus, closely related to O. fischeri and O. zhaoermii. These findings enhance our understanding of Hynobiidae diversity and evolution.

Mealybugs are aggressive pests worldwide, but few complete mitochondrial genomes have been reported. Here, we got the complete mitochondrial genome of the cotton mealybug, Phenacoccus solenopsis Tinsley, using Oxford Nanopore Technology. It is 14743 bp in length, encodes 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), and 2 ribosomal RNAs (rRNAs), and exhibits a high AT content of 90.33%. Maximum likelihood phylogenetic tree revealed that P. solenopsis is closely related to P. manihoti. This study presents the first complete mitogenome of a mealybug obtained through long-read sequencing, providing a valuable genomic resource for further studies of the mealybug family.