Mitochondrial DNA. Part B, Resources最新文献

Begonia pedatifida has persistently been utilized as a traditional folk herbal medicine. This study has sequenced the chloroplast genome of B. pedatifida to establish its genomic characteristics and to discern its phylogenetic relationships with other closely related species. The chloroplast genome structure of B. pedatifida reveals a circular molecule with a length of 169,606 bp, including a large single copy (LSC) region of 76,086 bp, a small single copy (SSC) region of 18,314 bp, and a pair of inverted repeats (IRS) region of 37,603 bp. The entire genome contains 138 genes, which consist of 88 protein-coding genes, 42 tRNA genes, and 8 rRNA genes. Phylogenetic analysis suggests that B. pedatifida is closely related to Begonia emeiensis, Begonia jinyunensis, and Begonia pulchrifolia, sharing a common ancestor and forming sister lineages. This research provides genetic information for further study on B. pedatifida.

Elaeocarpus duclouxii, an evergreen tree species, is renowned for its fruits rich in flavonoids exhibiting potent antioxidant properties. Despite its significance, the chloroplast genome of this plant has remained unexplored until now. Our study presents the first comprehensive sequencing and analysis of the E. duclouxii chloroplast genome, revealing a circular DNA molecule of 158,148 base pairs. This genome comprises a large single-copy region of 85,700 base pairs, a small single-copy region of 17,672 base pairs, and a pair of inverted repeat regions totaling 27,388 base pairs. The genome encodes 132 genes, including 87 protein-coding genes, 37 transfer RNA genes, and 8 ribosomal RNA genes. Phylogenetic analyses indicate a close evolutionary relationship between E. duclouxii and E. sylvestris. This study not only represents the first phylogenetic investigation of E. duclouxii but also establishes a crucial genomic foundation for future research area such as conservation genetics, evolutionary biology, and potential biotechnological applications.

Rorippa palustris Besser 1821, a species of Brassicaceae, is widely distributed around the world and used for both food and traditional Chinese medicinal purposes. Despite the plant's significance, its genetic diversity must be better understood. In this study, we have successfully assembled and characterized a complete plastome of R. palustris, marking a significant advancement toward comprehending its genetic composition. The plastome is 154,674 bp long and harbors 128 genes, including 83 protein-coding genes, 37 tRNA genes, and eight rRNA genes. Our phylogenomic analysis indicated that R. palustris is closely related to R. curvipes. These findings are crucial for conserving and utilizing this important plant species. They also highlight the potential for future research into the evolution and preservation of R. palustris, which could be advantageous in pharmaceutical applications.

In this study, the complete mitochondrial genome (mitogenome) of Cotylorhiza tuberculata (Scyphozoa; Rhizostomeae; Cepheidae) was assembled by the next-generation sequencing data. The complete mitogenome spanned 16,590 bp and contained 14 protein-coding genes, two transfer RNA genes, and two ribosomal RNA genes. Total AT% content was 67.7%, comprising A 30.22%, C 16.16%, G 17.05%, and T 36.56%. The gene arrangement exhibited consistency with the known mitogenomes of other jellyfish species. Furthermore, the phylogenetic relationship of C. tuberculata was investigated based on analysis of the 13 common protein-coding genes. Results indicated a close relationship between C. tuberculata and both Cassiopea xamachana and Cassiopea andromeda. These findings provide a valuable reference for advancing understanding of the phylogenetic relationships, taxonomic classification, and phylogeography of jellyfish species.

Mesembryanthemum cordifolium, a perennial plant with crassulacean acid metabolism (CAM) in the Aizoaceae family, has significant ornamental and medicinal values. In this study, we reported the first complete chloroplast genome sequence of this species. The total genome size was 153,734 bp in length, including a large single-copy (LSC) region of 85,692 bp, a small single-copy (SSC) region of 18,212 bp, and a pair of inverted repeat (IR) regions of 24,915 bp by each. The overall GC content of the M. cordifolium chloroplast genome was 37.08%. The genome encodes 131 genes, comprising 87 protein-coding genes (PCGs), 36 transfer RNA genes (tRNAs), and eight ribosomal RNA genes (rRNAs). Phylogenetic analysis shows this species was relatively close to M. crystallinum. This chloroplast genome sequence will be valuable for species discrimination and for understanding phylogenetic relationships within the genus Mesembryanthemum.

The complete mitochondrial genome of Leptychaster arcticus, deep-sea inhabited asteroid, was examined in this study. The complete mitogenome of L. arcticus is 16,253 bp in length and contains 13 protein-coding genes, 22 transfer RNA genes, and two ribosomal RNA genes. No gene rearrangements or deletions were observed in compared to other Paxillosida. The ND4L and ND3 genes have 'ATT' as its start codon, which is a feature that has been found in previous echinoderm mitochondrial studies. In the ML tree analysis based on the superorder Valvatacea, it was difficult to establish the molecular phylogenetic relationship at lower taxonomic levels, such as order and family, due to the lack of asteroid molecular data available. Therefore, we expect to contribute to the expansion of the data and determine the phylogenetic positioning in future studies.

The Manchurian hare (Lepus mandshuricus) is widely distributed in eastern Russia and northeastern China, but due to limited research, its taxonomic status remains somewhat ambiguous. The mitochondrial genome of the Manchurian hare was 16,705 bp in length, which was consisted of 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes and one control region. The overall nucleotide composition is 31.7% A, 29.4% T, 13.3% G, and 25.6% C, indicating a high AT content. Phylogenetic analysis reveals a closer relationship of the Manchurian hare with the Korean hare (Lepus coreanus) and the Iberian hare (Lepus granatensis), while its relationship with the Hainan rabbit (Lepus hainanus), European hare (Lepus europaeus) and the snowshoe hare (Lepus americanus) is more distant. The mitochondrial genome of the Manchurian hare is of vital importance for the phylogenetic analysis of lagomorphs and provides valuable data for deeper evolutionary inquiries.

Dryas octopetala L. var. asiatica (Nakai) Nakai 1918 is a dwarf shrub that mainly grow in alpine and arctic zones of the Northern Hemisphere, representing an endemic variety in Asia. In the present study, the complete chloroplast (cp) genome of D. octopetala var. asiatica was first characterized and used for its phylogenetic analysis. The cp genome span 158,271 bp with an overall GC content of 36.5%. A total of 129 genes were identified, including 84 protein-coding genes (PCGs), 37 tRNA genes, and 8 rRNA genes. In addition, repetitive sequences and microsatellites were detected within this species. Phylogenetic analysis involving 39 cp genomes from Rosaceae family indicated that D. octopetala var. asiatica was sister to the clade of Amygdaloideae. This study contributes fundamental insights into the cp genome of Dryas octopetala var. asiatica, which will have expanded its use in photosynthesis and evolutionary study.

Triplophysa grahami Regan 1906 is a member of the family Nemacheilidae, Cypriniformes, and native loach in Yunnan. In this study, the complete mitochondrial genome (mitogenome) of T. grahami Regan 1906 was firstly reported and analyzed. The mitogenome of T. grahami Regan 1906 is 16,566 bp in length, including 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs), and one control region (D-loop). The arrangement and orientation of protein coding genes and RNAs in T. grahami Regan 1906 are identical to other species of Nemacheilidae. The base composition of T. grahami Regan 1906 mitogenome was 29.25% A, 28.55% T, 25.03% C, and 17.17% G. The phylogenetic analysis based on the mitogenome showed that T. grahami Regan 1906 belongs to the clade of genus Triplophysa and the monophyly of Triplophysa is identified. This study contributed valuable genetic data for T. grahami Regan 1906 and explored the phylogenetic relationships in Nemacheilidae.

Chlaenius bimaculatus Dejean, 1826 (Coleoptera: Carabidea) is a predator of several lepidopteran pests, including Spodoptera frugiperda, S. litura and Helcystogramma triannulella. However, there has been little research into using C. bimaculatus to control crop pests. In this study, we sequenced the complete mitochondrial genome of C. bimaculatus. The results showed that the entire mitochondrial genome was 16,419 bp and contained 24% GC. 13 protein-coding, 22 transfer RNA, and two ribosomal RNA genes were identified. C. bimaculatus shares the same genetic arrangement and composition as other Coleoptera insects. In addition, phylogenetic analysis revealed that C. bimaculatus is closely related to Diplocheila zeelandica.