Mitochondrial DNA. Part B, Resources最新文献

Rhamnella franguloides (Maxim.) Weberb. (Rhamnaceae) is a shrub native to East Asia that supports forest understories, and has potential medicinal and ornamental value. In this study, the complete plastome of R. franguloides was sequenced and assembled. The 161,545 bp circular plastome showed a typical quadripartite structure consisting of a pair of inverted repeats (IRs; 26,723 bp), a large single-copy region (LSC; 88,828 bp), and a small single-copy region (SSC; 19,269 bp). It comprises 132 annotated genes including 87 protein-coding genes (PCGs), 37 tRNA genes, and 8 rRNA genes with an overall GC content of 37.1%. Phylogenetic analysis based on complete chloroplast genomes revealed that R. franguloides forms a well-supported sister relationship within the Rhamnella clade, indicating its intermediate phylogenetic position within the genus. This plastome sequence provides a valuable genomic resource for further taxonomic and evolutionary studies of the Rhamnaceae.

Strophanthus divaricatus (Lour.) Hook. & Arn. (1837) is an important medicinal plant utilized in traditional Chinese and Yao ethnomedicine. Here, we report its complete chloroplast genome based on Illumina sequencing. The circular genome is 155,409 bp in length, with an overall GC content of 38.28%, and displays a typical quadripartite structure comprising a large single-copy region (85,683 bp), a small single-copy region (18,182 bp), and two inverted repeats (25,772 bp each). A total of 130 genes were annotated, including 85 protein-coding genes, 37 tRNAs, and 8 rRNAs. Phylogenetic analysis revealed that S. divaricatus is closely related to S. wallichii.

Enicospilus ramidulus (Hymenoptera: Ichneumonidae) is a common parasitoid of Noctuidae larvae, with potential role in natural pest control. Here, we sequenced and annotated the complete mitochondrial genome of E. ramidulus. The mitochondrial genome spans 15,424 bp with an A + T content of 83.7% It comprises 13 protein-coding genes (PCGs), 22 tRNAs, two rRNAs, and a control region (CR). Phylogenetic analyses conducted using the maximum-likelihood method supported a close relationship between E. ramidulus and E. sp. within the subfamily Ophioninae. This study enhances our understanding of the mitochondrial genome structure of E. ramidulus, and contributes to the broader research field of Ichneumonidae genomics.

We report the complete mitochondrial genome of the halotolerant green alga Dunaliella salina CS-265, isolated from a hypersaline lake in central Australia. The genome is a circular DNA molecule of 30,073 bp, encoding seven protein-coding genes, nine rRNAs, and three tRNAs. Four core genes (cox1, cob, nad1, and nad5) are fragmented by multiple introns, whereas others remain intact. The absence of ATP synthase subunits and ribosomal protein genes reflects ongoing reductive evolution in Dunaliella mitochondria. This genome adds a new organellar resource from an Australian isolate, complementing previous studies and providing further insight into mitochondrial genome dynamics in halotolerant green algae.

Malus komarovii (Sarg.) Rehder is an endangered species endemic to China. Using Deep Genome Skimming (DGS) data, we assembled the complete chloroplast genome, which is 159,982 bp in length and comprises a Large Single-Copy (LSC, 88,119 bp), a Small Single-Copy (SSC, 19,157 bp), and two Inverted Repeats (IR, 26,353 bp each). The genome encodes 131 genes, including 86 protein-coding genes, 37 tRNA genes, and eight rRNA genes. Phylogenetic analyses show that M. komarovii forms a distinct lineage among the seven major clades, indicating pronounced genetic distinctiveness and potential value as a germplasm resource for breeding. This chloroplast genome provides valuable data for future studies on species identification and phylogenetic relationships within Malus.

The leafroller moths Cydia glandicolana and Cydia kurokoi are economically important pests. Here, we report the first complete mitochondrial genomes (mitogenomes) of these two species,with length of 15,395 bp and 15,246 bp, respectively. Each mitogenome contains13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and a single A + T-rich (D-loop) region. Their GC contents are 20.4% and 21.1%, respectively. Phylogenetic analysis, based on currently available sampling, supports the monophyly of the genus Cydia and indicates it is sister to the genus Leguminivora. Our study provides valuable genomic resources for taxonomic identification, biomonitoring, phylogenomics, and studies of genome evolution.

The snapping shrimp Alpheus digitalis De Haan, 1844 represents a commercially valuable fishery resource within the family Alpheidae. In this study, we sequenced and characterized the complete mitochondrial genome of A. digitalis, followed by phylogenetic reconstruction to elucidate its evolutionary relationships. The genome is 15,735 bp in length and contains 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), 2 ribosomal RNA genes (rRNAs), and 1 D-Loop control regions, with a pronounced A + T bias (60.66%). Phylogenetic analysis based on 13 PCGs robustly supports a sister-group relationship between A. digitalis and Alpheus hoplocheles. These findings furnish essential mitogenomic resources for advancing phylogenomic, taxonomic, and evolutionary investigations of caridean shrimps.

The complete mitochondrial genome of Leptobotia bellacauda was sequenced and characterized for the first time. The mitogenome was a 16,591 bp circular molecule, including 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA genes (rRNA), and a control region. Nucleotide composition: 24.4% A, 30.6% T, 28.6% C, and 16.4% G. Phylogenetic analysis confirmed the evolutionary position of L. bellacauda within its congeneric species and indicated that L. bellacauda was more closely related to L. punctata. This study provides crucial molecular biological evidence for investigating the taxonomy and phylogeny of L. bellacauda, and enhances our understanding of the molecular phylogeny and evolution of the genus Leptobotia.

Actinidia trichogyna is primarily distributed in the high-altitude regions of southwest China and its genomic data resources are still lacking. In this study, we assembled and annotated its complete chloroplast genome, which spanned 156,507 bp and consisted of a large single-copy region of 88,295 bp, a small single-copy region of 20,610 bp and two inverted repeat regions of 23,801 bp. The genome contained 131 genes, including 84 protein-coding genes, 8 ribosomal RNA genes, and 39 transfer RNA genes. Phylogenetic analysis revealed that it formed an independent branch to other species in the Sect. Maculatae, suggesting its unique evolutionary history. This study provides valuable data resources for kiwifruit evolutionary and functional genomic studies.

Berchemiella wilsonii var. pubipetiolata H. Qian, a member of the Rhamnaceae family, is endemic to mountainous regions in two eastern Chinese provinces. Here, we present the first assembly of its chloroplast (cp) genome, which exhibits a typical quadripartite structure, spans 160,317 bp long and has a 37.20% GC content. Genome annotation identified 131 genes, including 84 messenger RNA (mRNA) genes, 37 transfer RNA (tRNA) genes, and eight ribosomal RNA genes. Phylogenetic analysis confirms its close relation to B. wilsonii, thereby providing valuable genomic resources for conservation efforts and evolutionary studies of East Asian Berchemiella species.