Mitochondrial DNA. Part B, Resources最新文献

Fraxinus paxiana distributed in Shaanxi, Gansu, Hubei, Hunan, and Sichuan provinces, is used as a local medicine in Shaanxi and also serves as an adulterant of traditional Chinese medicine Qinpi. Here, we assembled its 155,692 bp chloroplast genome (GC content 37.87%) with a typical quadripartite structure. A total of 133 genes were annotated, including 88 protein-coding genes, 37 tRNA genes, and eight rRNA genes. We identified 43 SSRs, predominantly composed of mononucleotide repeats. Phylogenetic analysis showed that the genus Fraxinus is divided into two branches, with F. paxiana and F. insularis having the closest phylogenetic relationship.

Seseli giganteum Lipsky, an endemic Apiaceae species from the Kyrgyz Republic, was characterized based on its complete plastid genome. The circular genome is 147,554 bp long and exhibits a typical quadripartite structure, comprising an LSC (92,927 bp), SSC (17,631 bp), and two IRs (18,498 bp each). Phylogenetic analyses using 79 plastid protein-coding genes under maximum likelihood and Bayesian inference yielded highly congruent trees with strong support. The results indicate that Seseli L. is non-monophyletic, with Ligusticum L. and Peucedanum L. nested within it. S. giganteum is sister to S. valentinae Popov, highlighting a close Central Asian relationship and suggesting a need to reassess generic boundaries.

This study reports the first complete mitochondrial genome of Opsariichthys jishuiensis. The circular genome (16,614 bp) contains 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and a control region. Phylogenetic analysis using Bayesian inference and maximum-likelihood methods identifies O. jishuiensis as most closely related to O. acutipinnis and O. evolans, forming a monophyletic clade within the subfamily Xenocyprinae. These findings confirm the taxonomic placement of Opsariichthys and provide essential genetic resources for the evolutionary biology, taxonomy, and conservation of freshwater fishes in the Huanghe-Huaihe River Basin.

Actinidia melliana is a unique Actinidia species whose genomic data is extremely lacking. In this study, we reported the complete chloroplast genome of A. melliana for the first time, which has a total length of 156,159 bp, consisting of a large single-copy (LSC) region of 90,525 bp, a small single-copy (SSC) region of 20,578 bp, and a pair of inverted repeat (IR) regions with 22,528 bp each. A total of 133 genes were annotated, including 84 protein-coding genes, 41 transfer RNA genes (tRNA) and 8 ribosomal RNA genes (rRNA). Phylogenetic analysis alongside 28 other species in the Actinidiaceae family revealed that A. melliana is most closely related to A. cylindrica var. cylindrica. This study provides new genetic resources for evolutionary studies of Actinidia and supports future efforts in species identification and conservation within the genus.

The clearwing moth Synanthedon namdoelegans Kim, Kim and Choi, 2025 (Lepidoptera: Sesiidae) was newly discovered in South Korea in 2024. We sequenced 15,578 bp-long complete mitochondrial genome of the species. It contained the trnQ-trnS2-trnM-trnI arrangement at the A + T-rich region and ND2 junction (the underline indicates counterclockwise direction) unique to tribe Synanthedonini. Phylogenetic analyses of 18 Cossoidea species supported the monophyly of each Synanthedonini and Synanthedon, and revealed a sister relationship between S. namdoelegans and Synanthedon bicingulata (Staudinger 1887). These data will be useful in taxonomic, molecular ecological, and pest management research.

The Zygaenidae family comprises diurnal moths recognized for their bright coloration and chemical defenses. Despite its ecological and evolutionary significance, mitochondrial genome (mitogenome) data remain scarce. Here, we present the mitogenome of Neochalcosia remota, the first to be elucidated in members of the Neochalcosia genus, in the Chalcosiinae subfamily. The complete mitogenome is 15,246 bp and contains the standard set of 37 genes, with 13 protein-coding genes (PCGs), 22 tRNAs, and 2 rRNAs, as well as an A + T-rich region. An inverted translocation of the trnE-trnS₁ block (underlined gene names indicate a counter-clockwise transcriptional direction), instead of the typical trnS₁ -trnE arrangement in Lepidoptera, present in all Zygaenidae species, was also detected in N. remota. Phylogenetic analyses based on the 13 PCGs and two rRNAs in the Zygaenidae strongly supported the monophylies of this family and its subfamilies (Chalcosiinae, Procridinae, and Zygaeninae), placing N. remota as a sister taxon to Eterusia aedea. As a new contribution to the mitogenome database, the mitogenome of N. remota could provide valuable genetic data leading to a diverse taxonomic hierarchy in phylogenetic studies.

We sequenced and annotated the complete mitochondrial genome of Aoria bowringii. The circular mitogenome is 17,054 bp in length and exhibits a strong A + T bias, with nucleotide composition A 47.71%, T 30.63%, C 16.00%, and G 5.67%. It contains the typical set of 37 metazoan mitochondrial genes, including 13 protein-coding genes (PCGs), 22 transfer RNA genes, and two ribosomal RNA genes, as well as a putative control region. A maximum-likelihood phylogenetic analysis based on 13 PCGs places A. bowringii as a highly supported sister species to its congener Aoria nigripes. This mitogenome provides a valuable molecular resource for future phylogenetic and population genetic studies of this insect pest.

Eriocrania semipurpurella alpina Xu, 1990 is a leaf miner of birch trees in Qinghai Province, China. The complete mitogenome of E. semipurpurella alpina was reported in this study. The mitogenome contains 15,107 base pairs. Its nucleotide composition is 40.5% A, 8.1% G, 40.1% T, and 11.3% C. The genome encodes a set of 37 genes, including 13 protein-coding genes (PCGs), 22 tRNA genes, 2 rRNA genes, and one control region. Phylogenetic analysis supports the monophyly and primitive origin of Eriocraniidae.

The chloroplast genome size of Cardamine leucantha L. is 155,040 bp, including a large single-copy (LSC) region of 84,258 bp, a small single-copy (SSC) region of 17,936 bp, and two inverted repeats (IRs) of 26,423 bp, with an overall GC content of 36.33%. The chloroplast genome encodes 130 genes, comprising 85 protein-coding genes, eight rRNA genes, and 37 tRNA genes. Phylogenetic analysis using maximum-likelihood estimation indicates that C. leucantha is closely related to other species within the Cardamineae genus. The data from this study provide genomic resources for phylogenetic genetics and resource development of C. leucantha.

The fungal genus Ganoderma recently expanded with the description of Ganoderma shanxiense L. Fan & H. Liu 2019. This study presents the complete mitogenome of the species. The 97,036-bp circular mitogenome contains 60 functional genes, including 14 standard protein-coding genes, 27 tRNA genes, two rRNA genes, and 17 non-conserved ORFs. Notably, 32 introns are identified within 10 genes, harboring 31 intronic ORFs. Phylogenetic analysis based on mitochondrial protein sequences robustly places G. shanxiense within the Ganodermataceae family. This mitogenome provides a critical molecular resource for future taxonomic and evolutionary studies of this economically and ecologically significant fungal genus.