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Complete genome sequence of Desulfoscipio sp. strain L, a putative sulfate-reducing bacterium isolated from contaminated groundwater. 从受污染的地下水中分离出的硫酸盐还原细菌Desulfoscipio sp.菌株L的全基因组序列。
IF 0.7 Q4 MICROBIOLOGY Pub Date : 2026-02-12 Epub Date: 2025-12-23 DOI: 10.1128/mra.00451-25
Xiaocui Li, Hongyan Wang, Zongming Xiu, Haiwei Wei, Frank E Löffler, Yi Yang

We report the complete genome sequence of Desulfoscipio sp. strain L, a putative sulfate-reducing bacterium isolated from contaminated groundwater. The genome comprises a circular chromosome of 4,259,676 bp and a 1,530-bp circular plasmid.

我们报道了从受污染的地下水中分离出来的一种假定的硫酸盐还原细菌Desulfoscipio sp.菌株L的全基因组序列。该基因组包括一条4,259,676 bp的环状染色体和一个1,530 bp的环状质粒。
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引用次数: 0
Emergence of rare SARS-CoV-2 subvariant LF.7.3 obtained from Moroccan COVID-19 patients. 从摩洛哥COVID-19患者中获得的罕见SARS-CoV-2亚变体LF.7.3的出现。
IF 0.7 Q4 MICROBIOLOGY Pub Date : 2026-02-12 Epub Date: 2026-01-09 DOI: 10.1128/mra.00911-25
Hamza Ghammaz, Adil El Hamouchi, Nadia Touil, Hicham El Annaz, Marouane Melloul, Abdelhamid Barakat, Elmostafa El Fahime

We report the coding-complete genome sequence of a rare severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) subvariant (LF.7.3) identified in Morocco. Genomic analysis indicates that these strains belong to the LF.7.3 sublineage, a descendant of the JN.1 variant within the Omicron lineage.

我们报告了在摩洛哥发现的罕见的严重急性呼吸综合征冠状病毒2 (SARS-CoV-2)亚变体(LF.7.3)的编码完整基因组序列。基因组分析表明,这些菌株属于LF.7.3亚谱系,是Omicron谱系中JN.1变体的后代。
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引用次数: 0
Complete mitochondrial genome of the litchi pathogen Phytophthora litchii. 荔枝致病菌荔枝疫霉(Phytophthora litchii)线粒体全基因组。
IF 0.7 Q4 MICROBIOLOGY Pub Date : 2026-02-12 Epub Date: 2025-12-29 DOI: 10.1128/mra.01235-25
Jinhua Sun, Min Li, Gengxin Chen, Yajun Ran, Xianheng Lv, Meijiao Hu

The complete mitochondrial genome of the Phytophthora litchii strain hk-1 isolated from China was assembled and annotated. The circular genome has a length of 38,126 bp and is AT-rich (78.26%). It harbors two ribosomal RNA genes, 25 transfer RNA genes, and 41 protein-coding genes.

对中国产荔枝疫霉hk-1株线粒体全基因组进行了组装和注释。该环状基因组全长38126 bp, at丰富(78.26%)。它含有2个核糖体RNA基因,25个转移RNA基因和41个蛋白质编码基因。
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引用次数: 0
Complete genome sequence of Bacillus subtilis SNUY1, a novel strain enhancing antifungal activity in edible coatings. 枯草芽孢杆菌(Bacillus subtilis SNUY1)的全基因组序列研究
IF 0.7 Q4 MICROBIOLOGY Pub Date : 2026-02-12 Epub Date: 2025-12-23 DOI: 10.1128/mra.01061-25
Gwon Mo Yang, Na Yun Kim, Yohan Song, Donghwa Chung, Hyo Jin Kim

Bacillus subtilis SNUY1, isolated from Korean fermented food, strongly inhibits Botrytis cinerea. Its 4.19 Mb genome comprises 4,164 protein-coding genes and 108 pseudogenes (4,272 CDSs) without plasmids and includes 33 antifungal-related genes. These genetic traits highlight its potential as a natural food additive to control mold growth on perishable produce.

枯草芽孢杆菌(Bacillus subtilis SNUY1)从韩国发酵食品中分离得到,对灰葡萄孢具有较强的抑制作用。其4.19 Mb的基因组包含4164个蛋白质编码基因和108个无质粒的假基因(4272个CDSs),包括33个抗真菌相关基因。这些遗传特征突出了它作为一种天然食品添加剂控制易腐农产品霉菌生长的潜力。
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引用次数: 0
Draft genome sequence of a Klebsiella pneumoniae virulent bacteriophage vB_KpBD_211 isolated from wastewater in Dhaka, Bangladesh. 从孟加拉国达卡的废水中分离出的肺炎克雷伯菌毒力噬菌体vB_KpBD_211的基因组序列草图。
IF 0.7 Q4 MICROBIOLOGY Pub Date : 2026-02-12 Epub Date: 2025-12-23 DOI: 10.1128/mra.00909-25
Mst Morioum Sarkar, Tasnimul Arabi Anik, Sangita Ahmed

Klebsiella pneumoniae phage vB_KpBD_211, isolated from wastewater in Dhaka, Bangladesh, has a 47,938 bp double-stranded DNA genome (57.05% GC), assembled from Illumina MiSeq data, and lacks virulence or antibiotic resistance genes, supporting its potential in MDR K. pneumoniae therapy.

肺炎克雷伯菌噬菌体vB_KpBD_211从孟加拉国达卡的废水中分离出来,具有47,938 bp的双链DNA基因组(57.05% GC),由Illumina MiSeq数据组装,缺乏毒力或抗生素耐药基因,支持其在耐多药肺炎克雷伯菌治疗中的潜力。
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引用次数: 0
Draft genome sequences of Shewanella spp. isolated from the marine sediment in British Columbia, Canada. 绘制从加拿大不列颠哥伦比亚省海洋沉积物中分离的希瓦氏菌的基因组序列。
IF 0.7 Q4 MICROBIOLOGY Pub Date : 2026-02-12 Epub Date: 2025-12-23 DOI: 10.1128/mra.00976-25
Xiaoji Liu, Scott Hrycauk, Mruthula Narayan, Skyler Umlah

We present the draft genome sequences of 32 Shewanella spp. isolated from the marine sediment in British Columbia, Canada. The sediment was collected from and near a fallowed Atlantic salmon (Salmo salar) farm; thus, our research highlights the uniqueness of Shewanella spp. associated with the region.

我们报道了从加拿大不列颠哥伦比亚省海洋沉积物中分离的32种希瓦氏菌的基因组序列草图。这些沉积物是从一个休养的大西洋鲑鱼养殖场及其附近收集的;因此,我们的研究突出了与该地区相关的希瓦氏菌的独特性。
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引用次数: 0
Complete genome sequence of Bacillus cereus NBNT-070, isolated from activated sludge of a wastewater treatment facility. 从污水处理设施的活性污泥中分离出蜡样芽孢杆菌NBNT-070的全基因组序列。
IF 0.7 Q4 MICROBIOLOGY Pub Date : 2026-02-12 Epub Date: 2026-01-27 DOI: 10.1128/mra.01168-25
Yuxi Tian, Yong Min, Xiaoyan Liu, Yueying Wang, Yimin Qiu, Ben Rao, Ling Chen, Lei Zhu

Bacillus cereus is a widely distributed gram-positive bacterium that exhibits several notable characteristics. Here, we report the complete genome sequence of B. cereus strain NBNT-070, which was isolated from the activated sludge of a wastewater treatment facility. This data will be instrumental in elucidating the ecological role of B. cereus.

蜡样芽孢杆菌是一种广泛分布的革兰氏阳性细菌,具有几个显著特征。在这里,我们报告了蜡样芽孢杆菌菌株NBNT-070的全基因组序列,该菌株是从污水处理设施的活性污泥中分离出来的。这些数据将有助于阐明蜡样芽孢杆菌的生态作用。
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引用次数: 0
AmpliFuse: an amplicon simulation tool with enhanced chimera generation for Illumina platforms. AmpliFuse:用于Illumina平台的扩增子模拟工具,具有增强的嵌合体生成功能。
IF 0.7 Q4 MICROBIOLOGY Pub Date : 2026-02-12 Epub Date: 2026-01-27 DOI: 10.1128/mra.01439-25
Mingsong Kang

PCR-generated chimeric amplicons undermine the accuracy and reliability of subsequent amplicon-sequencing analyses. AmpliFuse, a Python-based tool, simulates realistic amplicon datasets through in silico PCR, chimera formation, and read simulation. By providing biologically relevant chimera-containing amplicon reads, AmpliFuse would facilitate benchmarking of chimera-detection algorithms and workflows, advancing microbiome and AMR research.

pcr产生的嵌合扩增子破坏了随后扩增子测序分析的准确性和可靠性。AmpliFuse是一个基于python的工具,通过硅PCR,嵌合体形成和读取模拟模拟现实的扩增子数据集。通过提供生物相关的嵌合体扩增子读取,AmpliFuse将促进嵌合体检测算法和工作流程的基准测试,推进微生物组和AMR研究。
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引用次数: 0
Microbiota composition of fruit flies and their environments in eastern USA orchards. 美国东部果园果蝇的微生物群组成及其环境。
IF 0.7 Q4 MICROBIOLOGY Pub Date : 2026-02-12 Epub Date: 2026-01-12 DOI: 10.1128/mra.01233-25
Wendy A Cisneros Cancino, Joseph T Gale, Aubrey Cluff, Amanda Morrison, Sarah J Gottfredson Morgan, Maggie Nosker, Jack K Beltz, Paul Schmidt, John M Chaston

We present a 16S rRNA analysis of the microbiota of fruit flies and their fruit and soil environments collected across a latitudinal gradient in the eastern USA. Collections varied according to fly taxonomy, location, sampling substrate, and starvation condition. These samples reveal variation in microbiota composition with several key variables.

我们提出了一个16S rRNA分析果蝇的微生物群和他们的果实和土壤环境收集跨纬度梯度在美国东部。根据蝇类分类、地点、取样基质和饥饿条件的不同,收集的蝇类有所不同。这些样品揭示了微生物群组成的几个关键变量的变化。
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引用次数: 0
PDIM/PGL-deficient Mycobacterium marinum shows disrupted protein secretion measured with bottom-up label-free quantitative analysis in data-dependent acquisition mode on a timsTOF Pro 2 mass spectrometer. 在timsTOF Pro 2质谱仪上,采用数据依赖获取模式,自下而上无标记定量分析显示PDIM/ pgl缺陷的海洋分枝杆菌蛋白分泌被破坏。
IF 0.7 Q4 MICROBIOLOGY Pub Date : 2026-02-12 Epub Date: 2025-12-29 DOI: 10.1128/mra.01161-25
Simon D Weaver, Daniel D Hu, Bradley S Jones, Patricia A Champion, Matthew M Champion

We investigated the chemical and functional consequences of a Mycobacterium marinum strain deficient in phthiocerol dimycocerosate and phenolic glycolipid (PDIM/PGL) virulence lipids using quantitative proteomics. The significant dysregulation of 1,006 proteins and altered ESAT-6 System-1 export and virulence were measured. PDIM/PGL deficiency specifically decreased the export of proteins with low isoelectric points.

我们利用定量蛋白质组学研究了一株海洋分枝杆菌缺乏硫代酚二真菌酸酯和酚醛糖脂(PDIM/PGL)毒力脂的化学和功能后果。测量了1006个蛋白的显著失调和ESAT-6 System-1输出和毒力的改变。PDIM/PGL缺陷特别减少了低等电点蛋白的输出。
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引用次数: 0
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