Pub Date : 2026-02-12Epub Date: 2025-12-29DOI: 10.1128/mra.00969-25
Said Laatri, Safaa El Kassimi, El Mehdi Bentaleb, MyDriss El Messaoudi, Bouchra Belkadi, Abdelkarim Filali-Maltouf, Hassan Ait Benhassou
We report draft whole-genome sequences of three extensively drug-resistant Mycobacterium tuberculosis strains (UM29, UM64, and UM66) isolated in Morocco in 2013. These assemblies support resistance profiling, molecular epidemiology, and comparative genomics. Raw sequencing reads and genome assemblies are publicly accessible through the NCBI database under BioProject PRJNA1302428.
{"title":"Draft genomes of three XDR <i>Mycobacterium tuberculosis</i> clinical isolates from Morocco.","authors":"Said Laatri, Safaa El Kassimi, El Mehdi Bentaleb, MyDriss El Messaoudi, Bouchra Belkadi, Abdelkarim Filali-Maltouf, Hassan Ait Benhassou","doi":"10.1128/mra.00969-25","DOIUrl":"10.1128/mra.00969-25","url":null,"abstract":"<p><p>We report draft whole-genome sequences of three extensively drug-resistant <i>Mycobacterium tuberculosis</i> strains (UM29, UM64, and UM66) isolated in Morocco in 2013. These assemblies support resistance profiling, molecular epidemiology, and comparative genomics. Raw sequencing reads and genome assemblies are publicly accessible through the NCBI database under BioProject PRJNA1302428.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0096925"},"PeriodicalIF":0.7,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12896266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145857225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-12Epub Date: 2026-01-16DOI: 10.1128/mra.00789-25
Gillian Tanabe, David R Mack, Alain Stintzi
The bacterium Phascolarctobacterium faecium frequently colonizes the human gut and has been reported to have reduced abundance in people with Crohn's disease. Here, we report the complete genome sequences of two P. faecium strains isolated from mucosal-luminal interface samples taken from pediatric participants with/without Crohn's disease.
{"title":"Complete genomes of <i>Phascolarctobacterium faecium</i> isolates obtained from pediatric mucosal-luminal interface aspirate samples.","authors":"Gillian Tanabe, David R Mack, Alain Stintzi","doi":"10.1128/mra.00789-25","DOIUrl":"10.1128/mra.00789-25","url":null,"abstract":"<p><p>The bacterium <i>Phascolarctobacterium faecium</i> frequently colonizes the human gut and has been reported to have reduced abundance in people with Crohn's disease. Here, we report the complete genome sequences of two <i>P. faecium</i> strains isolated from mucosal-luminal interface samples taken from pediatric participants with/without Crohn's disease.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0078925"},"PeriodicalIF":0.7,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12896173/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145989984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-12Epub Date: 2026-01-23DOI: 10.1128/mra.01181-25
Mautusi Mitra, Ana Stanescu
A novel Sphingobium yanoikuyae strain, CC4533, was isolated from a contaminated tris-acetate-phosphate medium culture plate of a green microalga, Chlamydomonas reinhardtii. In this study, we present the complete genome sequence of this beta-carotene-synthesizing, xenobiotic-degrading, and heavy metal-tolerant strain, providing insights into its metabolic capabilities and phylogenetic relationships.
{"title":"Complete genomic sequences of the single chromosome and four putative plasmids of <i>Sphingobium yanoikuyae</i> strain CC4533.","authors":"Mautusi Mitra, Ana Stanescu","doi":"10.1128/mra.01181-25","DOIUrl":"10.1128/mra.01181-25","url":null,"abstract":"<p><p>A novel <i>Sphingobium yanoikuyae</i> strain, CC4533, was isolated from a contaminated tris-acetate-phosphate medium culture plate of a green microalga, <i>Chlamydomonas reinhardtii</i>. In this study, we present the complete genome sequence of this beta-carotene-synthesizing, xenobiotic-degrading, and heavy metal-tolerant strain, providing insights into its metabolic capabilities and phylogenetic relationships.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0118125"},"PeriodicalIF":0.7,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12896234/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146030240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
During an investigation of Burkholderia species from agricultural soils in Mexico, Burkholderia semiarida was isolated from the rhizosphere of Theobroma cacao L. growing in Chiapas, Mexico. Here, we present the draft genome of Burkholderia semiarida CLM7-1.
{"title":"Draft genome sequence of <i>Burkholderia semiarida</i> isolated from <i>Theobroma cacao</i> in Chiapas, Mexico.","authors":"Nadia-Paola Ortiz-Conde, Leslie-Mariana Morales-Ruíz, Jeniffer-Chris Kerber-Diaz, Violeta Larios-Serrato, Paulina Estrada-de Los Santos","doi":"10.1128/mra.01377-25","DOIUrl":"https://doi.org/10.1128/mra.01377-25","url":null,"abstract":"<p><p>During an investigation of <i>Burkholderia</i> species from agricultural soils in Mexico, <i>Burkholderia semiarida</i> was isolated from the rhizosphere of <i>Theobroma cacao</i> L. growing in Chiapas, Mexico. Here, we present the draft genome of <i>Burkholderia semiarida</i> CLM7-1.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0137725"},"PeriodicalIF":0.7,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146166155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-12Epub Date: 2025-12-19DOI: 10.1128/mra.01229-25
Alexey V Beletsky, Egor A Vasyagin, Maxim Yu Shalamitskiy, Elena V Ivanova, Nikolai V Ravin, Andrey V Mardanov
We report the complete genome sequence of the wine yeast strain Saccharomyces cerevisiae I-118. It was isolated from yeast sediment formed after spontaneous fermentation of Rkatsiteli grapes and is used to produce white wines. Genomic data provide the basis for linking the genotype of this strain with its oenological properties.
{"title":"Сomplete genome sequence of <i>Saccharomyces cerevisiae</i> I-118, a wine strain isolated from Rkatsiteli grapes in Uzbekistan.","authors":"Alexey V Beletsky, Egor A Vasyagin, Maxim Yu Shalamitskiy, Elena V Ivanova, Nikolai V Ravin, Andrey V Mardanov","doi":"10.1128/mra.01229-25","DOIUrl":"10.1128/mra.01229-25","url":null,"abstract":"<p><p>We report the complete genome sequence of the wine yeast strain <i>Saccharomyces cerevisiae</i> I-118. It was isolated from yeast sediment formed after spontaneous fermentation of Rkatsiteli grapes and is used to produce white wines. Genomic data provide the basis for linking the genotype of this strain with its oenological properties.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0122925"},"PeriodicalIF":0.7,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12896240/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145794054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-12Epub Date: 2026-01-12DOI: 10.1128/mra.01117-25
Jun Yonetamari, Masahiro Hayashi, Yoshinori Muto, Tetsuya Iida, Kaori Tanaka
The genus Cellulomonas comprises aerobic, Gram-positive, rod-shaped bacteria commonly present in the soil and aquatic environments. Herein, we report the isolation of Cellulomonas sp. GTC20042 from human bile and the complete genome sequence of this strain, consisting of a 4,145,753-bp circular chromosome.
{"title":"Complete genome sequence of <i>Cellulomonas</i> sp. GTC 20042 isolated from human bile.","authors":"Jun Yonetamari, Masahiro Hayashi, Yoshinori Muto, Tetsuya Iida, Kaori Tanaka","doi":"10.1128/mra.01117-25","DOIUrl":"10.1128/mra.01117-25","url":null,"abstract":"<p><p>The genus <i>Cellulomonas</i> comprises aerobic, Gram-positive, rod-shaped bacteria commonly present in the soil and aquatic environments. Herein, we report the isolation of <i>Cellulomonas</i> sp. GTC20042 from human bile and the complete genome sequence of this strain, consisting of a 4,145,753-bp circular chromosome.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0111725"},"PeriodicalIF":0.7,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12896168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145952577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-12Epub Date: 2025-12-29DOI: 10.1128/mra.01124-25
Tony X Liu, Sean Formby, Beth Davenport, Resmi Capron, Andras Szeitz, Steven J Hallam
Here, we report on the complete genome sequence of Escherichia coli EPI300. This strain harbors an inducible plasmid copy number control system supporting DNA library production and functional screening applications. PacBio long-read sequencing and assembly resolved a closed circular reference genome useful for mapping and editing the EPI300 genetic operating system.
{"title":"Complete genome sequence of <i>Escherichia coli</i> EPI300.","authors":"Tony X Liu, Sean Formby, Beth Davenport, Resmi Capron, Andras Szeitz, Steven J Hallam","doi":"10.1128/mra.01124-25","DOIUrl":"10.1128/mra.01124-25","url":null,"abstract":"<p><p>Here, we report on the complete genome sequence of <i>Escherichia coli</i> EPI300. This strain harbors an inducible plasmid copy number control system supporting DNA library production and functional screening applications. PacBio long-read sequencing and assembly resolved a closed circular reference genome useful for mapping and editing the EPI300 genetic operating system.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0112425"},"PeriodicalIF":0.7,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12896278/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145850152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-12Epub Date: 2026-01-14DOI: 10.1128/mra.01311-25
Sabrina Wamp, Sven Halbedel
Here, we present the closed genome sequences of nine representative clinical Listeria monocytogenes isolates from nine invasive listeriosis outbreaks in Germany that were associated with contaminated salmon products. More than half of the genomes contained two or more intact prophages.
{"title":"Closed genome sequences of clinical <i>Listeria monocytogenes</i> isolates from salmon-associated listeriosis outbreaks.","authors":"Sabrina Wamp, Sven Halbedel","doi":"10.1128/mra.01311-25","DOIUrl":"10.1128/mra.01311-25","url":null,"abstract":"<p><p>Here, we present the closed genome sequences of nine representative clinical <i>Listeria monocytogenes</i> isolates from nine invasive listeriosis outbreaks in Germany that were associated with contaminated salmon products. More than half of the genomes contained two or more intact prophages.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0131125"},"PeriodicalIF":0.7,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12896246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145966456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-12Epub Date: 2026-01-27DOI: 10.1128/mra.01351-25
Martha Giles-Gómez, Diego Guerrero Corona, Elizabeth Martínez García, Francisco Bolívar, Adelfo Escalante
We report the complete genome sequences of three endophytic Pseudomonas putida isolates from Agave salmiana, assembled using Illumina NovaSeq and PacBio HiFi sequencing. Genome sizes were 6,282,325 bp (PP_P5), 6,511,513 bp (PP_P9), and 6,284,694 bp (PP_P43), respectively.
我们报道了从salmiana Agave中分离的三个内生恶臭假单胞菌的全基因组序列,使用Illumina NovaSeq和PacBio HiFi测序进行组装。基因组大小分别为6,282,325 bp (PP_P5), 6,511,513 bp (PP_P9)和6,284,694 bp (PP_P43)。
{"title":"Complete genomes of three endophytic <i>Pseudomonas putida</i> isolates from <i>Agave salmiana</i>, assembled using Illumina and PacBio sequencing.","authors":"Martha Giles-Gómez, Diego Guerrero Corona, Elizabeth Martínez García, Francisco Bolívar, Adelfo Escalante","doi":"10.1128/mra.01351-25","DOIUrl":"10.1128/mra.01351-25","url":null,"abstract":"<p><p>We report the complete genome sequences of three endophytic <i>Pseudomonas putida</i> isolates from <i>Agave salmiana</i>, assembled using Illumina NovaSeq and PacBio HiFi sequencing. Genome sizes were 6,282,325 bp (PP_P5), 6,511,513 bp (PP_P9), and 6,284,694 bp (PP_P43), respectively.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0135125"},"PeriodicalIF":0.7,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12896289/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146052972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-12Epub Date: 2025-12-23DOI: 10.1128/mra.01232-25
Mark J Rozmarynowycz, Katelyn M Brown, J D Gantz, Richard E Lee, Arthur Zastepa, Sue Watson, R Michael L McKay
Ice and water microbial communities from Lake Winnipeg were explored through V4 region 16S rRNA gene sequencing during a pronounced period of cold temperatures over North America's Great Plains and Great Lakes regions. Diatoms, cyanobacteria, and Xanthomonadales (Gammaproteobacteria) displayed patterns of partitioning into ice fractions.
{"title":"Amplicon sequencing of ice and water phytoplankton and bacterial communities during an extreme winter in a central Canadian great lake.","authors":"Mark J Rozmarynowycz, Katelyn M Brown, J D Gantz, Richard E Lee, Arthur Zastepa, Sue Watson, R Michael L McKay","doi":"10.1128/mra.01232-25","DOIUrl":"10.1128/mra.01232-25","url":null,"abstract":"<p><p>Ice and water microbial communities from Lake Winnipeg were explored through V4 region 16S rRNA gene sequencing during a pronounced period of cold temperatures over North America's Great Plains and Great Lakes regions. Diatoms, cyanobacteria, and Xanthomonadales (Gammaproteobacteria) displayed patterns of partitioning into ice fractions.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0123225"},"PeriodicalIF":0.7,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12896203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145809886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}