Sung Min Ha, Ethan Humm, Neil Q Wofford, Jessica R Sieber, Liudmilla Rubbi, Giorgia Del Vecchio, Matteo Pellegrini, Michael J McInerney, Robert P Gunsalus
We report the genome sequences of two syntrophic gram-negative bacteria, Syntrophomonas erecta GB4-38T and Syntrophomonas erecta subsp. sporosyntropha 5-3-ZT, which degrade C4-C8 short-chain fatty acids when grown in co-culture with a suitable hydrogen-consuming microbial strain.
{"title":"Genomes of <i>Syntrophomonas erecta</i> GB4-38<sup>T</sup> and <i>Syntrophomonas erecta</i> subsp. <i>sporosyntropha</i> 5-3-Z<sup>T</sup> that degrade C4-8 short-chain fatty acids.","authors":"Sung Min Ha, Ethan Humm, Neil Q Wofford, Jessica R Sieber, Liudmilla Rubbi, Giorgia Del Vecchio, Matteo Pellegrini, Michael J McInerney, Robert P Gunsalus","doi":"10.1128/mra.01419-25","DOIUrl":"https://doi.org/10.1128/mra.01419-25","url":null,"abstract":"<p><p>We report the genome sequences of two syntrophic gram-negative bacteria, <i>Syntrophomonas erecta</i> GB4-38<sup><b>T</b></sup> and <i>Syntrophomonas erecta</i> subsp. <i>sporosyntropha</i> 5-3-Z<sup><b>T</b></sup>, which degrade C4-C8 short-chain fatty acids when grown in co-culture with a suitable hydrogen-consuming microbial strain.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0141925"},"PeriodicalIF":0.7,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146165269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-12Epub Date: 2025-12-22DOI: 10.1128/mra.01001-25
Beth Davenport, Ryan McLaughlin, Tony Liu, Steven J Hallam
We report the complete genome sequence of Caulobacter vibrioides CB2A JS4038. This strain is a variant of C. vibrioides CB2A containing genotypic changes needed to host a plasmid-encoded S-layer display system. PacBio long-read sequencing and assembly provide a closed reference genome, constraining the chromosomal location of these changes.
{"title":"Complete genome sequence of <i>Caulobacter vibrioides</i> CB2A JS4038.","authors":"Beth Davenport, Ryan McLaughlin, Tony Liu, Steven J Hallam","doi":"10.1128/mra.01001-25","DOIUrl":"10.1128/mra.01001-25","url":null,"abstract":"<p><p>We report the complete genome sequence of <i>Caulobacter vibrioides</i> CB2A JS4038. This strain is a variant of <i>C. vibrioides</i> CB2A containing genotypic changes needed to host a plasmid-encoded S-layer display system. PacBio long-read sequencing and assembly provide a closed reference genome, constraining the chromosomal location of these changes.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0100125"},"PeriodicalIF":0.7,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145804920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-12Epub Date: 2025-12-19DOI: 10.1128/mra.01160-25
Junseok Bang, Byeollee Kim, Yung Mi Lee, Hackwon Do, Jun Hyuck Lee, Tae-Jin Oh
We report the complete genome sequence of Rahnella sp. PAMC25559, isolated from Zugspitz glacier, Austria. The complete genome assembly was generated, consisting of one chromosome and two putative plasmid sequences. The genome includes biosynthetic gene clusters potentially related to cold adaptation and plant growth-promoting activity.
{"title":"Complete genome sequence of <i>Rahnella</i> sp. PAMC25559, a psychrophilic plant growth-promoting bacterium isolated from an Alpine glacier.","authors":"Junseok Bang, Byeollee Kim, Yung Mi Lee, Hackwon Do, Jun Hyuck Lee, Tae-Jin Oh","doi":"10.1128/mra.01160-25","DOIUrl":"10.1128/mra.01160-25","url":null,"abstract":"<p><p>We report the complete genome sequence of <i>Rahnella</i> sp. PAMC25559, isolated from Zugspitz glacier, Austria. The complete genome assembly was generated, consisting of one chromosome and two putative plasmid sequences. The genome includes biosynthetic gene clusters potentially related to cold adaptation and plant growth-promoting activity.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0116025"},"PeriodicalIF":0.7,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145793815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-12Epub Date: 2025-12-23DOI: 10.1128/mra.01210-25
Prakash Chandra Shil, Md Abdullah Evna Hasan, Maysha Islam, Layla Yasmin, Najmun Nahar Popy, Md Bahanur Rahman, Mohammad Ferdousur Rahman Khan
We report the draft genome sequence of Mammaliicoccus sciuri strain BAU-MFRK-Mam-2025 isolated from phuchka samples in Mymensingh, Bangladesh. The 2.78 Mb genome (GC 32.8%, genome coverage 50×) harbors multiple antibiotic resistance genes, underscoring its potential threat to food safety, antimicrobial resistance dissemination, and public health.
Here, we report the complete genome sequencing of a new Mycobacterium sp. nov. from a compromised Japanese individual with hematological malignancy. This will represent the essential data for future phylogenetic and comparative genome studies and will be useful for better understanding the evolution of the pathogen.
{"title":"Complete genome sequence of a new <i>Mycobacterium</i> sp. nov. from a compromised Japanese individual.","authors":"Nobuhiro Asai, Yuriko Igarashi, Narimi Miyazaki, Asami Osugi, Yuki Matsumoto, Shota Nakamura, Satoshi Mitarai, Hiroshige Mikamo","doi":"10.1128/mra.00421-25","DOIUrl":"10.1128/mra.00421-25","url":null,"abstract":"<p><p>Here, we report the complete genome sequencing of a new <i>Mycobacterium</i> sp. nov. from a compromised Japanese individual with hematological malignancy. This will represent the essential data for future phylogenetic and comparative genome studies and will be useful for better understanding the evolution of the pathogen.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0042125"},"PeriodicalIF":0.7,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145850172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Peribacillus frigoritolerans is a widely distributed gram-positive bacterium that exhibits several notable characteristics. Here, we report the complete genome sequence of the P. frigoritolerans strain NBTC-101, which was isolated from the rhizosphere soil of melons. This genome sequence will help elucidate the ecological role of P. frigoritolerans.
{"title":"Complete genome sequence of <i>Peribacillus frigoritolerans</i> NBTC-101, isolated from melon rhizosphere soil.","authors":"Wei Chen, Wenrui Liu, Yong Min, Ben Rao, Yuxi Tian, Fei Li, Qian Deng, Yueying Wang, Xiaoyan Liu, Lei Zhu, Ling Chen","doi":"10.1128/mra.00937-25","DOIUrl":"10.1128/mra.00937-25","url":null,"abstract":"<p><p><i>Peribacillus frigoritolerans</i> is a widely distributed gram-positive bacterium that exhibits several notable characteristics. Here, we report the complete genome sequence of the <i>P. frigoritolerans</i> strain NBTC-101, which was isolated from the rhizosphere soil of melons. This genome sequence will help elucidate the ecological role of <i>P. frigoritolerans</i>.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0093725"},"PeriodicalIF":0.7,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145857177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-12Epub Date: 2025-12-29DOI: 10.1128/mra.00969-25
Said Laatri, Safaa El Kassimi, El Mehdi Bentaleb, MyDriss El Messaoudi, Bouchra Belkadi, Abdelkarim Filali-Maltouf, Hassan Ait Benhassou
We report draft whole-genome sequences of three extensively drug-resistant Mycobacterium tuberculosis strains (UM29, UM64, and UM66) isolated in Morocco in 2013. These assemblies support resistance profiling, molecular epidemiology, and comparative genomics. Raw sequencing reads and genome assemblies are publicly accessible through the NCBI database under BioProject PRJNA1302428.
{"title":"Draft genomes of three XDR <i>Mycobacterium tuberculosis</i> clinical isolates from Morocco.","authors":"Said Laatri, Safaa El Kassimi, El Mehdi Bentaleb, MyDriss El Messaoudi, Bouchra Belkadi, Abdelkarim Filali-Maltouf, Hassan Ait Benhassou","doi":"10.1128/mra.00969-25","DOIUrl":"10.1128/mra.00969-25","url":null,"abstract":"<p><p>We report draft whole-genome sequences of three extensively drug-resistant <i>Mycobacterium tuberculosis</i> strains (UM29, UM64, and UM66) isolated in Morocco in 2013. These assemblies support resistance profiling, molecular epidemiology, and comparative genomics. Raw sequencing reads and genome assemblies are publicly accessible through the NCBI database under BioProject PRJNA1302428.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0096925"},"PeriodicalIF":0.7,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145857225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study reports the draft genome of an Acinetobacter baumannii strain niger01 co-producing blaNDM-1, blaOXA-23, and blaOXA-69 genes. The genome size is 4,084,803 bp, 39.0% GC content, and harbors several insertion sequences. The emergence of new high-risk clones presents an escalating problem that significantly challenges the public health system.
{"title":"Draft genome of <i>Acinetobacter baumannii</i> strain niger01 co-producing <i>bla</i><sub>NDM-1</sub> and <i>bla</i><sub>OXA-23</sub> genes in Niger.","authors":"Abdourahamane Yacouba, Oumou Hamidou, Ahmed Olowo-Okere, Abdoulaye Ousmane, Massir Issoufou-Noma, Bassirou Abdou-Natali, Ounoussa Tapha, Mahamadou Doutchi, Saidou Mamadou","doi":"10.1128/mra.01300-25","DOIUrl":"10.1128/mra.01300-25","url":null,"abstract":"<p><p>This study reports the draft genome of an <i>Acinetobacter baumannii</i> strain niger01 co-producing <i>bla</i><sub>NDM-1</sub>, <i>bla</i><sub>OXA-23</sub>, and <i>bla</i><sub>OXA-69</sub> genes. The genome size is 4,084,803 bp, 39.0% GC content, and harbors several insertion sequences. The emergence of new high-risk clones presents an escalating problem that significantly challenges the public health system.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0130025"},"PeriodicalIF":0.7,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145857237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-12Epub Date: 2026-01-12DOI: 10.1128/mra.01155-25
Zuzana Sebechlebska, Michal Ziemski, Nicholas A Bokulich
Technical hurdles are a significant barrier for deposition of next-generation sequence data in public repositories. We present q2-ena-uploader, a software package for automated validation and upload of sequence data. It is BSD-3-licensed and available at https://github.com/bokulich-lab/q2-ena-uploader.
{"title":"FAIRer DNA sequence data with q2-ena-uploader: a QIIME 2 plugin for data deposition in the European Nucleotide Archive.","authors":"Zuzana Sebechlebska, Michal Ziemski, Nicholas A Bokulich","doi":"10.1128/mra.01155-25","DOIUrl":"10.1128/mra.01155-25","url":null,"abstract":"<p><p>Technical hurdles are a significant barrier for deposition of next-generation sequence data in public repositories. We present q2-ena-uploader, a software package for automated validation and upload of sequence data. It is BSD-3-licensed and available at https://github.com/bokulich-lab/q2-ena-uploader.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0115525"},"PeriodicalIF":0.7,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145952619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-02-12Epub Date: 2026-01-13DOI: 10.1128/mra.00953-25
Md Amdadul Huq, M Mizanur Rahman, Md Shahedur Rahman
This study reports the draft genome sequence of Flavobacterium sp. MAHUQ-57, a strain isolated from the soil sample of a rice field located in Dighalgram, Magura, Bangladesh, in 2019. The genome consists of a 3,472,804 base pair chromosome assembled into 23 contigs, encoding 3,202 predicted protein-coding genes.
{"title":"Genome sequence of <i>Flavobacterium</i> sp. MAHUQ-57 isolated from the soil sample of a rice field located in Dighalgram, Magura, Bangladesh.","authors":"Md Amdadul Huq, M Mizanur Rahman, Md Shahedur Rahman","doi":"10.1128/mra.00953-25","DOIUrl":"10.1128/mra.00953-25","url":null,"abstract":"<p><p>This study reports the draft genome sequence of <i>Flavobacterium</i> sp. MAHUQ-57, a strain isolated from the soil sample of a rice field located in Dighalgram, Magura, Bangladesh, in 2019. The genome consists of a 3,472,804 base pair chromosome assembled into 23 contigs, encoding 3,202 predicted protein-coding genes.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0095325"},"PeriodicalIF":0.7,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145959718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}