Minerva dental and oral science最新文献

The began of the dentistry as medicine's concept has a long history dating back to 7000 BCE, making it one of the earliest medical specialties. In its early days, dental diseases were often attributed to supernatural causes like "evil spirits" and "tooth worms." However, today, dentistry leverages cutting-edge technologies, including artificial intelligence, for diagnosis and treatment. This journey reflects the remarkable progress made in the field. Despite these advancements, there is still room for improvement in integrating dental knowledge and skills with medical science and engineering backgrounds. Bridging these disciplines could lead to even greater advancements in the diagnosis and maintenance of oral health. Thus, over time, it has transformed from a primitive form of medicine into a modern field that emphasizes preventative dental care, advanced diagnostics, and state-of-the-art treatment.

Background: Temporomandibular disorders (TMDs) are a heterogeneous group of conditions involving the temporomandibular joint complex, and surrounding musculature and osseous components. They are a multifactorial disease that can be determined by organic, genetic aspects, oral parafunctional habits, and psychological stress. They have an annual incidence of more than 5% of the population and about 6% to 12% of the population is affected by symptoms. The diagnostic criteria (DC), introduced by Dworkin, is considered the standard system to diagnose this disease in a specific way. Imaging can support the diagnosis of TMD when history and physical examination findings are equivocal. The aim of the study was to evaluate instrumental examinations and therapies, clinicians prefer to use in different cases of TMDs.

Methods: An anonymized survey, available in two languages (Italian and English), was given to 450 patients, 398 (120 males, 274 females and 4 who did not answer) of different private dental practices were considered, using Google Form (Google LLC, Mountain View, CA, USA) and used an electronic platform, from September 2021 to February 2022. There was no reminder sent to patients to let them feel free to answer.

Results: We performed binary regression for oral bite prescription considering common symptoms and found that the most representative is jaw block (P=0.007, exponential value [EXP]=0.509), followed by TMJ noises (P=0.01, EXP=0.503) and feeling stressed (P=0.04, EXP=1.125) while headache and tinnitus resulted not significant.

Conclusions: The study highlighted that magnetic resonance imaging (MRI) is the most instrumental examination adopted by the clinicians and the oral splint(bite) is the therapy most widespread nowadays, even if TMDs are a multifactorial disease that is not, probably, only linked to an alteration of dental occlusion.

Background: Periodontal disease poses a significant global health challenge. Traditional treatments focus on reducing inflammation and bacterial load, yet novel approaches are continually being investigated. Recent research suggests that IL-37, a potent anti-inflammatory cytokine, may play a crucial role in modulating the inflammatory processes associated with periodontal disease. In conjunction with IL-37, low-level laser therapy (LLLT) has gained attention for its potential in promoting tissue repair, reducing inflammation, and enhancing cellular processes. This study aims to investigate the effects of LLLT on IL-37 in periodontal disease management.

Methods: Thirty patients were enrolled: the G1 group patients were treated with only scaling and root planning-SRP, the G2 group was treated with SRP and LLLT. Before treatment (T0) all periodontal probing pocket depth and bleeding on probing were obtained. Before (T0) and 10 (T1), 30 (T2) and 60 (T3) days after treatment, was achieved plaque sample and specimens of gingival crevicular fluid. Diode laser wavelength range was used between 600-1000 nm and 0.04-60 J/cm² energy density for 3-s spotlights.

Results: In all patients PPD, BOP and IL-37 have shown healing improved parameters.

Conclusions: Although LLLT is widely recommended for its biostimulatory and anti-inflammatory roles, it only showed additional short-term merits in reducing the pocket depth after conventional SRP. Its long-term adjunctive benefits remain unclear. Future RCTs with better study designs, adequate sample power and longer durations of follow-up are required to assess the effectiveness of LLLT as an adjunctive treatment strategy in patients with periodontal disease.

Background: Yogurt, due to its rich casein phosphopeptide (CPP) content could promote remineralization of enamel. Contrary to the age-old usage of animal milk based yogurt, vegan dairy products are gaining momentum due to multiple reasons. Considering this change, the aim of the present study was to assess the in vitro effect of animal and plant-based yogurt extracts on demineralization of enamel.

Methods: Enamel windows were prepared on the crowns of sixty premolar teeth using nail paint. Teeth were divided into four groups of fifteen each and were treated with distilled water, demineralizing agent, solution with a mixture of demineralizing agent and yogurt supernatants, respectively for 96 hrs. Quantitative analysis was done using EDXRF (baseline and postexperimental calcium and phosphorus content). Additionally, confocal microscopic analysis was performed to assess the extent of demineralization.

Results: The animal-based yogurt (Group III) had the highest post experimental calcium value (mean±SD=81.15±5.02) and positive percentage of change in calcium level (15%; P=0.007) among the groups. This was followed by plant-based yogurt (Group IV) (mean calcium= 76.18±5.12; positive percentage change =8.11%; P=0.003).

Conclusions: Animal based yogurt could provide higher protection against enamel demineralization when compared to plant-based yogurt.

Background: Primary stability of dental implant is an important prerequisite for achieving osseointegration. This study was conducted to evaluate the effects of photobiomodulation therapy on bone formation-around implants by measuring the implant removal torque and implant stability quotient.

Methods: This study was conducted in six adult male sheep. Four implants were placed on each side of the lower border of the mandible. The implant beds were prepared to a size of 10 mm in length and 4.8 mm in width, to receive an implant of 8 mm in length and 4 mm in width. Laser application to the socket was performed just before implant placement, and was immediately administered to the surface of the implant and the peri-implant bone before suturing of the wound. The therapy was continued twice daily for the next seven consecutive days. The animals were sacrificed at 4, 8, and 12 weeks, with two animals per time point. The implant-removal torque was determined with an electronic wrench, and the implant stability quotient (ISQ) was assessed with an Ostell device.

Results: The laser treated sides showed significantly higher removal torque and ISQ, at the three-time points (P<0.05). At 4 weeks, the ISQ was 61.44 (±10.4) in the laser group and 48.2 (±16.7) in the control group. At 8 weeks, the ISQ increased to 62.2 (±5.5) in the laser group and 56.1 (±4.3) in the control group. At 12 weeks, the ISQ was 67 (±4.5) in the laser group and 61.875 (±6.3) in the control group. The removal torque at 4 weeks was 218.6 (±62.6) in the laser group and 147.6 (±40.9) in the control group. At 8 weeks, the removal torque increased to 370.5 (±33.3) in the laser group and 250.2 (±25.0) in the control group. At 12 weeks, the removal torque increased to 912.6 (±177.2) in the laser group and 512.1 (±122.6) in the control group.

Conclusions: Photobiomodulation enhances bone formation and improves implant stability in implants with overzealously prepared oversized implant beds.

Background: The aim of the current study was to propose a classification of impacted cuspids, and bicuspids based on their spatial position in the maxillary and mandibular arches and their radiological appearance.

Methods: This prospective study was conducted including all the patients who reported to our center for extractions, orthodontic treatment, missing permanent cuspids/bicuspids, removal of third molar teeth. All patients who reported within a time frame of 6 months were enrolled in the study. Orthopantomogram was advised for all the patients as a part of diagnostic work up. The panoramic radiographs and clinical data were reviewed and patients with evidence of impacted bicuspids and cuspids were included in the study and were advised to undergo a cone beam computed tomography to evaluate the 3-dimensional position of the impacted teeth. Treatment plan was decided based on the position of the impacted teeth and feasibility for orthodontic movement. Standard surgical protocol was followed for all the patients. The duration of the procedure from the time of incision till the suturing were tabulated and the difficulty of extraction was correlated with the current Difficulty Index to validate the same.

Results: A total number of 4165 patients were enrolled in the study and based on the inclusion and exclusion criteria, 3680 patients were excluded and 487 patients with impacted cuspids/bicuspids were included in the study. A total number of 231 patients had impacted maxillary cuspids and bicuspids and 256 cases had impacted mandibular cuspids/bicuspids. Amongst the 256 mandibular cuspid and bicuspid that were impacted, 62% were in position C while the rest of 38% were in position B. More than ⅓ of the teeth which were in position C had transmigrated (35%). Seventy-three percent of the maxillary teeth were in position C and 69% of these teeth were either transmigrated (29%), or were horizontally (38%) placed in the alveolus or inverted (2%). The rest of the 31% were found to be either mesially inclined or vertical. The average Difficulty Index for maxillary teeth was 8 and mandibular teeth was 9. Mandibular cuspids and bicuspids in position B and C took longer time for surgical removal compared to the maxillary teeth and this was statistically significant.

Conclusions: The proposed clinical classification on impacted mandibular cuspids and bicuspids provides a structured approach to plan the treatment based on the 3-dimensional position of the teeth in the arch.

Introduction: Ossification disorders can affect the stylohyoid ligament as well the stylomandibular ligament. The symptomathology between those syndromes and other pathologies it has been a problem because there is not a guideline.

Evidence acquisition: With the aim to establish prevalence, diagnosis and etiopathogenesis of Eagle and Ernest syndromes, a scoping review was performed base on PRISMA criteria.

Evidence synthesis: To review stylohyoid ligament and Eagle Syndrome 93 articles were selected. Three articles regarding the stylomandibular ligament and Ernest Syndrome were founded and selected.

Conclusions: Both syndromes should be clinically diagnosed and corroborate with imaging (2D or 3D), although imaging in Ernest Syndrome is not clear as well in Eagle Syndrome. A decisional algorithm for diagnosis of Eagle Syndrome and Ernest Syndrome is presented.

Background: The purpose of this study was to compare the color stability and marginal adaption of lithium disilicate veneers before and after thermocycling using various resin composite materials employed as cement.

Methods: Laminate veneer preparation was done on an acrylic tooth according to standardized procedures. The veneer preparations were duplicated into twenty-one dies. The veneers were fabricated from lithium disilicate using the CEREC in Lab system. According to the cement type, the twenty-one die specimens were randomly divided into three equal groups: group I, with light cured adhesive resin cement (N.=7), group II, with flowable light cured composite, and group III, with preheated nano filled composite. The cemented specimens underwent 5000 thermocycling cycles. Color was assessed using a reflective spectrophotometer. The vertical marginal gap, following cementation and thermocycling, was measured using a digital microscope. One-way ANOVA followed by Tukey's post-hoc test was used to statistically assess the data.

Results: After thermocycling, there was no statistically significant difference in ΔE among cement type groups for color stability (P=0.369). However, the preheated composite showed the lowest ΔE mean values. Within adhesive resin cement and flowable composite groups, marginal gap means values recorded after thermocycling were significantly higher than those after cementation (P=0.013 and P<0.001, respectively).

Conclusions: Although preheated composite cement showed the highest marginal discrepancy, it would be the best choice regarding color stability.

Background: The purpose of the current study was to evaluate the effect of Diode LLLT 650 nm, TEMPO oxidized Nano-fibrillated cellulose mixed with Nano-Amorphous calcium phosphate, and their combination on bone healing in rabbit tibia using H&E staining and computed tomography.

Methods: Eighteen adult male New Zealand rabbits were selected, two circular bone defects were created in each tibia, resulting in four bony defects in each rabbit, representing the four tested groups; group A (negative control), group B (filled with mineralized nano-cellulose), group C (combination), group D (laser). Animals were euthanized after two weeks and one month, defects were assessed by CT for bone density, then histological samples were examined by H&E stain.

Results: In both evaluation periods, group D recorded the greatest mean area percent of new bone formation and bone density, followed by group A, while group C recorded the lowest value. Groups A and D showed full closure of the defects, while groups B and C showed partial defect closure with retained bone graft material. H&E and CT showed that Laser group had the best results of defects healing, bone density and new bone formation, followed by the negative control group.

Conclusions: Diode laser 650nm photobiomodulation significantly improved bone defects healing. Mineralized nano-cellulose experimental bone substitute material showed a delayed effect in bone healing and graft material resorption. The combination of LLLT with the graft material had no positive outcome on bone defect healing.