Minerva dental and oral science最新文献

Exfoliative cytology has proven to be a valuable diagnostic tool in the early detection of malignant neoplasms. However, its application in the oral cavity has been met with skepticism and limited investigation due to the perception that clinical examination alone is sufficient for early diagnosis. Nonetheless, recent research efforts have focused on the utility of exfoliative cytology in oral cavity neoplasms, motivated by the high mortality rate associated with oral cancer. This manuscript reviews the historical development of exfoliative cytology in the oral cavity, highlighting key findings and advancements in the field. Early studies demonstrated the presence of neoplastic cells in oral cavity lesions and emphasized the diagnostic potential of cytology. Subsequent research explored different staining methods and observed the differential reaction of oral cavity cells to radiation therapy. The diagnostic accuracy of exfoliative cytology was reported to be as high as 92%, with a significant number of cases diagnosed as carcinomas without any clinical suspicion. The technique also proved valuable in identifying precancerous and dysplastic lesions and obviating the need for unnecessary biopsies. The manuscript delves into the difficulties encountered in sampling techniques and stresses the significance of appropriate specimen collection and handling. Various staining methods, including Papanicolaou and acridine orange, were utilized for cytological analysis. The manuscript emphasizes the extensive use of exfoliative cytology in oral cavity examinations and stresses the importance of recognizing it as a crucial diagnostic tool. Various studies have reported high positivity rates and the detection of clinically unsuspected malignant lesions. The authors recommend extensive use of exfoliative cytology in routine practice and underscore the necessity of proper sampling and evaluation techniques.

Background: Oral cancer is a predominant and aggressive form of head and neck cancer with limited treatment options. Stevioside, a naturally occurring biocompatible compound, has gained attention for its potential therapeutic properties, although its molecular mechanistic role in OSCC merely understood. This study aims to elucidate the impact of stevioside on OSCC cells, focusing on its inhibitory effects on cell proliferation and epithelial-mesenchymal transitions (EMT).

Methods: KB cells, representative of OSCC, were subjected to stevioside treatment in a time-dependent manner. The findings obtained from the MTT assay revealed a notable suppression of KB cell growth following 48 hours of treatment with stevioside.

Results: The IC50 values, which represent the concentration at which the inhibitory effect of stevioside is at 50% at 110.54 µM, as determined by this assay, were subsequently utilized further for the analysis of gene expression through RT-PCR. Furthermore, our investigation involved the examination of EMT-related genes (ECADH, SNAIL1, SLUG, and VIM) using molecular docking analysis.

Conclusions: In conclusion, this study sheds light on the significant role of stevioside in oral cancer. The observed inhibition of KB cell proliferation and its pronounced impact on EMT-related gene expression indicate the potential of stevioside as a promising therapeutic agent in the context of OSCC. The multifaceted effects of stevioside on OSCC cells present an exciting avenue for advancing our understanding of this devastating disease and the development of innovative therapeutic strategies.

Introduction: The number of retracted articles in the bio-medical field, including dentistry, is increasing. The objective of this study was to identify retracted articles in endodontic-related literature and outline their main characteristics.

Evidence acquisition: The Medline and Scopus databases were searched in January 2024. Additional records were identified by consulting the online database www.retractiondatabase.org. Retracted articles concerning endodontic topics and the relative retraction notes were retrieved and relevant data extracted.

Evidence synthesis: Sixty articles were identified, published between 2004 and 2022, with a mean of 3.0±2.3 retracted articles per year. The number of retracted articles per year did not significantly grow over time. The average time between the article and retraction note publication was 36.1±22.3 months. The majority of articles originated from Asia and were in-vitro studies. Only 6.7% of the articles were retracted due to honest errors. The main reasons of retraction were image/data manipulation (20%), plagiarism (18%), unavailability of raw data (10%), authorship issues (10%) and publication process issues (10%). A single research group accounted for 17 retractions.

Conclusions: Unlike the dental field, the number of retracted articles in endodontics has not increased significantly over the time period considered. Despite this, controls against research misconduct must be increased to preserve the credibility of scientific research.

Background: The objective of the in vitro study was to evaluate the impact of two adhesive removal techniques on the surface roughness of the human enamel surface using laser scanning confocal microscopy (LSCM).

Methods: Forty healthy first upper molar teeth were included in this experiment (20 teeth per each group). T0 measurement of surface roughness parameter of the teeth were conducted using LSCM. The teeth were bonded with metal brackets. Following 24 hours of sample storage in distilled water, the brackets were debonded. A modified adhesive remnant index (ARI) was used to exclude samples with below 90% adhesive remnants on their surface. The remaining 36 teeth were then randomly divided into two groups (18 teeth per group) with the following debonding procedures: group TC (single stage method using a Smoozie tungsten carbide bur) and group TCP (two stage method using a Smoozie tungsten carbide bur, followed by a Smoozie polisher). The time needed for complete adhesive removal was recorded and T1 surface roughness measurements were conducted again after adhesive removal using LSCM.

Results: Significantly higher surface roughness values were recorded in group TC, compared to group TCP (P<0.01). Method TCP, on the other hand, was significantly more time-consuming than method TC (P<0.01).

Conclusions: Despite the fact that the two-stage method (group TCP) was more time consuming, the lower level of enamel surface roughness in this method suggests the superiority of this method.

The aims of the present case report are to describe the non-surgical management in addition to hyaluronic acid application of two gingival recessions with cleft and to perform a narrative review on the newest evidence of non-surgical treatment of gingival recessions. A 47-year-old female patient with dentine hypersensitivity and pain during brushing has type 1 gingival recession with red Stillman's cleft on 1.4 and 1.5. The gingival defects have been treated with a Gracey curette n° 7/8 to cause bleeding. Sodium hyaluronate gel has been applied in the gingival sulcus of both elements affected. Three months after treatment the gingival tissue on 1.5 and 1.4 revealed complete healing of the Stillman's cleft and the mean value of REC depth reduced by 0.50 mm. Furthermore, four articles about this topic were selected for a narrative review. Based on the recent literature, the non-surgical treatment of Stillman's clefts and gingival recessions is not highly rated. The clinical result of this case report suggests a non-surgical method to treat Stillman's cleft. However, the evidence from a single case report is weak and more clinical trials are required to deepen the knowledge on the topic.

Background: Anxiety and fear are significant challenges in pediatric dentistry, often leading to avoidance of dental treatment. Some studies suggest a potential link between the dopamine beta-hydroxylase gene and the ABO gene. As ABO blood group type is determined genetically, it could serve as a bioindicator for assessing the impact of genetics on personality traits. The association between blood types and personality characteristics has long been debated.

Methods: Eighty eligible children, meeting the inclusion criteria, were enrolled in the study. Blood group data were obtained from parents/guardians. Dental anxiety assessment was done using the RMS-Picture scale and pulse oximeter.

Results: The collected data were analyzed statistically.

Conclusions: The study found no correlation between dental anxiety and ABO blood grouping.

Background: In recent years, there has been a gradual increase in cases of dentin hypersensitivity, often related to gingival recession, enamel abrasion from brushing, and wear of root cementum. This condition can affect patients of all ages but is more common between 20 and 40 years of age, especially in females and individuals with periodontal disease. The study aimed to evaluate and compare the efficacy over time of a product for at-home treatment of dentin hypersensitivity.

Methods: One hundred five patients aged between 28 and 65 years were recruited, each with at least two hypersensitive teeth due to erosions, abrasions, gingival recessions, and/or periodontal diseases. Participants were selected through the Schiff Test and anamnestic evaluations, with compilation of periodontal records. Patients are instructed to use the product three times a day, to be applied with a soft-bristled toothbrush and/or topically as a gel on teeth with strong sensitivity and deep cervical lesions and gingival recessions.

Results: There were no statistically significant differences between measurements at baseline and after scaling. However, significant differences were observed in follow-ups from the 1^st to the 8^th week, with a progressive decrease in values. The average trend of product efficacy increases over time according to the Schiff Test, with a significant improvement observed at follow-ups conducted after four and eight weeks.

Conclusions: The results indicate that the product enriched with biomimetic hydroxyapatite and lactoferrin is effective in reducing the painful symptoms of dentin hypersensitivity and in counteracting the inflammatory state of the supporting tissues.

The field of immediate loading implantology has grown rapidly, offering quicker solutions for prosthetic rehabilitation. However, the complexity of this technique raises significant medico-legal concerns regarding patient safety, treatment planning, and professional liability. These issues are particularly relevant in elective procedures where failure could lead to litigation. A comprehensive review of current literature, including clinical studies, legal rulings, and malpractice claims, was conducted to assess the legal implications of immediate loading implantology. The analysis focused on professional liability, patient consent, and complications during treatment. The evidence indicates that a high standard of care, including detailed preoperative planning, risk assessment, and clear patient communication, is essential in minimizing legal risks. Malpractice claims often arise from inadequate patient information or failure to follow established protocols. Proper documentation of informed consent and adherence to evidence-based dentistry (EBD) guidelines are crucial to protect both patients and practitioners. Immediate loading implantology offers numerous benefits, but it requires meticulous planning and patient management to avoid medico-legal issues. Ensuring comprehensive patient consent and following evidence-based practices are key to reducing professional liability and improving outcomes. Further studies should explore standardized protocols for reducing complications and legal disputes in implantology.

Background: In daily surgical practice, the dentist deals with various types of oral surgery. At the first visit, the patient fills out a questionnaire about their state of health. If the questionnaire does not reveal any particular hematological problems, no special tests are usually required to determine the platelet count. The aim of this prospective study was to evaluate the healing process of patients and its correlation with platelet levels before surgery.

Methods: A total of 50 patients were included in whom blood chemistry tests were performed with special attention to the platelet count and before their mandibular eights were extracted .The type of healing (EHI) and pain on the second day (VAS) were assessed using methods validated in the literature.

Results: After the EHI and VAS scores were noted in the medical records, envelopes containing the platelet scores of the previously requested blood samples, which were not read at the time of observation, were opened. An increase in EHI scores and a decrease in VAS scores was observed that were statistically significantly correlated with increasing platelet scores.

Conclusions: These results suggest that the dentist should request chemistry tests on preoperative blood, especially if the patient is to undergo a particularly demanding oral surgical procedure, such as implants, guided bone regeneration or complex extractions.

Background: Removable partial denture (RPD) is still a widely used prosthesis today to solve non-implant cases, despite its aesthetic contraindications. The aim of the following work is to evaluate how much the communication and explanation to the patient of the insertion axis of the removable partial prosthesis in the periodontium and teeth has an impact on his degree of acceptance of the aesthetic limits of this prosthesis.

Methods: For this study, 150 patients were examined from 10 dentists, 5 in Lombardy and 5 in Sicily, using inclusion criteria and exclusion criteria. All selected patients required removable partial dentures. The data was obtained by completing 2 questionnaires, at three different times, at prosthesis delivery, at 6 and 12 months.

Results: Questionnaires highlighted overlapping data in the two regions and a total absence of communication of the insertion axis by the dental technician laboratories was highlighted with a percentage of 100%. 28% of the patients complained of pain during the insertion of the prosthesis at 12 months and 39.33% of patients had mobility of remaining teeth with clasps at 12 months. All patients experienced no discomfort, and no important data were reported at the first 6-month follow-up.

Conclusions: For mobile partial dentures, respecting the insertion axis can improve the predictability of the periodontal tissue and the resistance of the remaining teeth with clasps. The drawing of an arrow in the model shows the insertion axis of the prosthesis. It would be desirable to include information regarding the insertion axis and advice for the management of the removable partial denture in the first days of use in the declaration of conformity given to the doctor and to thepatient, in the indications section. It can be said that correct insertion of the removable partial denture, helped by verbal and written instructions from the doctor, can reduce possible periodontal alterations of the remaining teeth, by eliminating spurious stresses during insertion and removal of the prosthesis.