Pub Date : 2024-09-06DOI: 10.23736/S2724-6329.24.04852-6
Marco DI Blasio, Giuseppe Minervini, Marzia Segù, Giuseppe Pedrazzi, Alberto DI Blasio, Diana Cassi, Marco Cicciù, Bendetta Vaienti
Background: It is well known that in bidimensional photography the orientation of the head is fundamental to obtain a correct reproduction of the subject. Even minimal errors in yaw, roll and pitch of the head can be cause of unreliability of the photography itself. This is not the case in three-dimensional photography in which head positioning may have no influence on the reliability of the results since on the computer the 3D reconstruction of the face can be oriented as desired, allowing any small errors to be corrected. The authors, in a previous in-vitro study, demonstrated this using a mannequin of the head. In the present study, the Authors repeat the study in vivo to verify any differences.
Methods: Seventeen anthropometric points were marked on the face of a young adult female (26 years old) with eyeliner. Images of the face were then acquired with a stereo-photogrammetric system (Face Shape 3D MaxiLine) in natural head position and with varying degrees of roll, pitch and yaw. The anthropometric points marked on the skin were then recognized and marked on a digital software (Viewbox, dHAL Software, 6 Menandrou Street, Kifissia 14561, Greece) on each individual image. 14 linear distances between these points were measured using the same software.
Results: The linear distances of these points in the stereophotos taken at centered positions were then compared with those taken with different degrees of roll, pitch and yaw. This study confirms previous results obtained in vitro regarding the presence of areas of non-equivalence (P<0.05) when comparing the frontal and lateral regions of the face at various angles.
Conclusions: However, it introduces important suggestions on the collection of data from specific anthropometric points. If these points are to be assessed, the medical staff will have to take special precautions to obtain reliable images.
{"title":"An in-vivo study on the effect of head orientation in the measurement of anthropometric points in stereophotogrammetry.","authors":"Marco DI Blasio, Giuseppe Minervini, Marzia Segù, Giuseppe Pedrazzi, Alberto DI Blasio, Diana Cassi, Marco Cicciù, Bendetta Vaienti","doi":"10.23736/S2724-6329.24.04852-6","DOIUrl":"https://doi.org/10.23736/S2724-6329.24.04852-6","url":null,"abstract":"<p><strong>Background: </strong>It is well known that in bidimensional photography the orientation of the head is fundamental to obtain a correct reproduction of the subject. Even minimal errors in yaw, roll and pitch of the head can be cause of unreliability of the photography itself. This is not the case in three-dimensional photography in which head positioning may have no influence on the reliability of the results since on the computer the 3D reconstruction of the face can be oriented as desired, allowing any small errors to be corrected. The authors, in a previous in-vitro study, demonstrated this using a mannequin of the head. In the present study, the Authors repeat the study in vivo to verify any differences.</p><p><strong>Methods: </strong>Seventeen anthropometric points were marked on the face of a young adult female (26 years old) with eyeliner. Images of the face were then acquired with a stereo-photogrammetric system (Face Shape 3D MaxiLine) in natural head position and with varying degrees of roll, pitch and yaw. The anthropometric points marked on the skin were then recognized and marked on a digital software (Viewbox, dHAL Software, 6 Menandrou Street, Kifissia 14561, Greece) on each individual image. 14 linear distances between these points were measured using the same software.</p><p><strong>Results: </strong>The linear distances of these points in the stereophotos taken at centered positions were then compared with those taken with different degrees of roll, pitch and yaw. This study confirms previous results obtained in vitro regarding the presence of areas of non-equivalence (P<0.05) when comparing the frontal and lateral regions of the face at various angles.</p><p><strong>Conclusions: </strong>However, it introduces important suggestions on the collection of data from specific anthropometric points. If these points are to be assessed, the medical staff will have to take special precautions to obtain reliable images.</p>","PeriodicalId":18709,"journal":{"name":"Minerva dental and oral science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142140561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-01Epub Date: 2024-07-04DOI: 10.23736/S2724-6329.24.04979-9
Domenico Ciavarella, Mauro Lorusso, Martina Leone, Donatella Ferrara, Carlotta Fanelli, Gaetano Illuzzi, Eleonora Ortu, Lorenzo Lo Muzio, Michele Tepedino
Background: The objective of this study was to evaluate the risk of maxillary canine impaction through cephalometric evaluation.
Methods: One hundred and eighty-two Caucasian patients (80 males and 102 females) with a mean age of 12.4±0.5 were retrospectively enrolled in the present study. Ninety patients with impacted canines were compared with ninety-two patients with physiologically erupted canines. The following cephalometric variables were analyzed: SNA, ANB, SN-GOME, GO-ME, SP-CL, SELLA TURCICA DEPHT, A-Pt LINE, B-Pt LINE, A-Pt LINE°, B-Pt LINE°, A1s-Pt, A1i-Pt, A1s-Pt°, A1i-PT°, A-SN, B-SN and SN-U1. When applicable, a paired sample t-test was performed to investigate the difference in means between the two sample groups, while the Mann-Whitney Test was used as a non-parametric test in the case of a non-normal distribution of data. Statistical significance was set at P<0.05.
Results: The group with impacted canines showed an increase in Go-Me distance, SNA angle and A1s-Pt angle compared with the control group. In addition, the same group showed a decrease in A-SN and SP-Cl distance compared with the control group.
Conclusions: An increase in mandibular length (GO-ME) and SNA angle associated with a reduction in A-Sn distance and interclinoid distance (SP-Cl) were observed in patients with impacted canines.
{"title":"Craniofacial morphology in patients with impacted canine: a case control-study.","authors":"Domenico Ciavarella, Mauro Lorusso, Martina Leone, Donatella Ferrara, Carlotta Fanelli, Gaetano Illuzzi, Eleonora Ortu, Lorenzo Lo Muzio, Michele Tepedino","doi":"10.23736/S2724-6329.24.04979-9","DOIUrl":"10.23736/S2724-6329.24.04979-9","url":null,"abstract":"<p><strong>Background: </strong>The objective of this study was to evaluate the risk of maxillary canine impaction through cephalometric evaluation.</p><p><strong>Methods: </strong>One hundred and eighty-two Caucasian patients (80 males and 102 females) with a mean age of 12.4±0.5 were retrospectively enrolled in the present study. Ninety patients with impacted canines were compared with ninety-two patients with physiologically erupted canines. The following cephalometric variables were analyzed: SNA, ANB, SN-GOME, GO-ME, SP-CL, SELLA TURCICA DEPHT, A-Pt LINE, B-Pt LINE, A-Pt LINE°, B-Pt LINE°, A1s-Pt, A1i-Pt, A1s-Pt°, A1i-PT°, A-SN, B-SN and SN-U1. When applicable, a paired sample t-test was performed to investigate the difference in means between the two sample groups, while the Mann-Whitney Test was used as a non-parametric test in the case of a non-normal distribution of data. Statistical significance was set at P<0.05.</p><p><strong>Results: </strong>The group with impacted canines showed an increase in Go-Me distance, SNA angle and A1s-Pt angle compared with the control group. In addition, the same group showed a decrease in A-SN and SP-Cl distance compared with the control group.</p><p><strong>Conclusions: </strong>An increase in mandibular length (GO-ME) and SNA angle associated with a reduction in A-Sn distance and interclinoid distance (SP-Cl) were observed in patients with impacted canines.</p>","PeriodicalId":18709,"journal":{"name":"Minerva dental and oral science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141498440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-01Epub Date: 2024-06-13DOI: 10.23736/S2724-6329.24.04996-9
Alessandro Motta, Gennaro Musella, Tommaso Dai Prà, Andrea Ballini, Mario Dioguardi, Maria E Bizzoca, Lorenzo Lo Muzio, Stefania Cantore
This review provides practical recommendations for dental practitioners in dealing with oral lesions associated with sexually transmitted diseases (STDs), offering clinically relevant insights to increase the awareness of these diseases in the mainstream of everyday practice. STDs are acquired through unprotected vaginal, anal or oral sex and are caused by more than 30 different types of bacteria, viruses and protozoa. Saliva, precum, semen, vaginal secretions and menstrual blood could be likely vehicles of infections and defensive barriers to infection by pathogenic microbes could be represented via intact mucosal membrane, the diluent function of saliva, and the antimicrobial action of salivary enzymes that collectively contribute to oral health and protection. STD, can directly and indirectly affect mucous membranes, manifesting with characteristic diagnostic signs and lesions. Given their potential oral manifestations, dental professionals need a comprehensive understanding of STD. The findings of this review lay a foundation for comprehending several STDs, emphasizing the importance of physicians as well dental practitioners being open to discussing sexuality issues with patients and providing appropriate therapeutic interventions.
{"title":"The literature discusses oral manifestations caused by sexually transmitted viruses: a narrative review.","authors":"Alessandro Motta, Gennaro Musella, Tommaso Dai Prà, Andrea Ballini, Mario Dioguardi, Maria E Bizzoca, Lorenzo Lo Muzio, Stefania Cantore","doi":"10.23736/S2724-6329.24.04996-9","DOIUrl":"10.23736/S2724-6329.24.04996-9","url":null,"abstract":"<p><p>This review provides practical recommendations for dental practitioners in dealing with oral lesions associated with sexually transmitted diseases (STDs), offering clinically relevant insights to increase the awareness of these diseases in the mainstream of everyday practice. STDs are acquired through unprotected vaginal, anal or oral sex and are caused by more than 30 different types of bacteria, viruses and protozoa. Saliva, precum, semen, vaginal secretions and menstrual blood could be likely vehicles of infections and defensive barriers to infection by pathogenic microbes could be represented via intact mucosal membrane, the diluent function of saliva, and the antimicrobial action of salivary enzymes that collectively contribute to oral health and protection. STD, can directly and indirectly affect mucous membranes, manifesting with characteristic diagnostic signs and lesions. Given their potential oral manifestations, dental professionals need a comprehensive understanding of STD. The findings of this review lay a foundation for comprehending several STDs, emphasizing the importance of physicians as well dental practitioners being open to discussing sexuality issues with patients and providing appropriate therapeutic interventions.</p>","PeriodicalId":18709,"journal":{"name":"Minerva dental and oral science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141311126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-01Epub Date: 2024-06-13DOI: 10.23736/S2724-6329.24.04950-7
Francesco S Ludovichetti, Nicoletta Zerman, Edoardo Stellini, Giulia Zambon, Sergio Mazzoleni, Andrea Zuccon
Background: Teeth bleaching is a common practice performed by dental hygienists and dentists to correct aesthetic problems due to dental discoloration. Patients' opinions on this procedure may be different according to their personal experiences, expectations and individual sensitivities. The aim of this study is to assess the degree of satisfaction and related sensitivity of patients to professional and home bleaching treatment.
Methods: A questionnaire consisting of 12 multiple-choice questions was submitted to 625 subjects among general population. These were related to: 1) patient's age and whether the patient is a smoker; 2) personal satisfaction; and 3) post-treatment tooth sensitivity. Descriptive analyses of all variables in the questionnaire were performed, and statistical analyses between variables were carried out by Chi-square test and Fisher's Exact test.
Results: Of the people surveyed, 66% performed home bleaching, while the remaining performed the treatment in a dental office. Thirty-three percent of patients had previously undergone teeth bleaching and the 95.7% stated that they were satisfied with the treatment. Of these, only 36.4% reported experiencing postoperative hypersensitivity. Most patients (91.3%) received professional scaling before undergoing bleaching treatment.
Conclusions: Patient satisfaction with tooth whitening treatment can vary from patient to patient and represents a strong indicator of treatment efficacy and suitability. A proper communication, careful management and individualized patient assessment are key to maximizing satisfaction and ensuring a positive and fulfilling patient experience.
{"title":"Dental bleaching: patient perception and satisfaction.","authors":"Francesco S Ludovichetti, Nicoletta Zerman, Edoardo Stellini, Giulia Zambon, Sergio Mazzoleni, Andrea Zuccon","doi":"10.23736/S2724-6329.24.04950-7","DOIUrl":"10.23736/S2724-6329.24.04950-7","url":null,"abstract":"<p><strong>Background: </strong>Teeth bleaching is a common practice performed by dental hygienists and dentists to correct aesthetic problems due to dental discoloration. Patients' opinions on this procedure may be different according to their personal experiences, expectations and individual sensitivities. The aim of this study is to assess the degree of satisfaction and related sensitivity of patients to professional and home bleaching treatment.</p><p><strong>Methods: </strong>A questionnaire consisting of 12 multiple-choice questions was submitted to 625 subjects among general population. These were related to: 1) patient's age and whether the patient is a smoker; 2) personal satisfaction; and 3) post-treatment tooth sensitivity. Descriptive analyses of all variables in the questionnaire were performed, and statistical analyses between variables were carried out by Chi-square test and Fisher's Exact test.</p><p><strong>Results: </strong>Of the people surveyed, 66% performed home bleaching, while the remaining performed the treatment in a dental office. Thirty-three percent of patients had previously undergone teeth bleaching and the 95.7% stated that they were satisfied with the treatment. Of these, only 36.4% reported experiencing postoperative hypersensitivity. Most patients (91.3%) received professional scaling before undergoing bleaching treatment.</p><p><strong>Conclusions: </strong>Patient satisfaction with tooth whitening treatment can vary from patient to patient and represents a strong indicator of treatment efficacy and suitability. A proper communication, careful management and individualized patient assessment are key to maximizing satisfaction and ensuring a positive and fulfilling patient experience.</p>","PeriodicalId":18709,"journal":{"name":"Minerva dental and oral science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141311125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-01Epub Date: 2024-07-02DOI: 10.23736/S2724-6329.24.04879-4
Mohammad S Alrashdan, Hisham Al-Shorman, Ahmed Al-Dwairi, Abubaker Qutieshat, Mahmoud K Al-Omiri
Background: Salivary oxidative stress has been extensively studied with attempts to correlate changes in the oxidative stress markers with local and systemic factors, including smoking. The objective of this study was to evaluate the influence of two forms of smoking, cigarettes and waterpipe smoking (WPS), on selected oxidative stress biomarkers in saliva.
Methods: Three groups of participants were enrolled into the study, controls (never smokers), cigarette smokers and WPS. Participants were clinically free from periodontitis and systemic conditions known to affect the saliva constituents. Unstimulated whole saliva samples were collected according to a standard protocol and concentrations of 8-hydroxy-2'-deoxyguanosine (8-OHdG), myeloperoxidase (MPO), malondialdehyde (MDA), total antioxidant capacity (TAC), and cortisol. The one-way ANOVA test was used to compare the levels of each oxidative stress biomarker between the three study groups and the hierarchical linear regression analysis was used to test the levels of salivary cortisol for prediction of other oxidative stress biomarkers. Significance levels were set at 95% confidence intervals and probability values ≤0.05.
Results: 8-OHdG was highest in WPS group (mean±SE 11,030.35±1829.16 pg/mL) while MDA and cortisol levels were highest in the cigarette smokers group (mean±SE 3.33±0.52 µM and 3.99±0.48 ng/mL, respectively) and MPO was highest in the control group (mean±SE 7.760±1.55 ng/mL). WPS group showed the highest TAC (mean±SE 0.3±0.03 mM). However, none of the tested makers reached a statistically significant difference.
Conclusions: Despite subtle changes in some biomarkers, the salivary oxidative stress does not appear to be significantly influenced by smoking habits in periodontitis-free smokers.
{"title":"Salivary oxidative stress biomarkers in periodontitis-free smokers: a cross sectional study.","authors":"Mohammad S Alrashdan, Hisham Al-Shorman, Ahmed Al-Dwairi, Abubaker Qutieshat, Mahmoud K Al-Omiri","doi":"10.23736/S2724-6329.24.04879-4","DOIUrl":"10.23736/S2724-6329.24.04879-4","url":null,"abstract":"<p><strong>Background: </strong>Salivary oxidative stress has been extensively studied with attempts to correlate changes in the oxidative stress markers with local and systemic factors, including smoking. The objective of this study was to evaluate the influence of two forms of smoking, cigarettes and waterpipe smoking (WPS), on selected oxidative stress biomarkers in saliva.</p><p><strong>Methods: </strong>Three groups of participants were enrolled into the study, controls (never smokers), cigarette smokers and WPS. Participants were clinically free from periodontitis and systemic conditions known to affect the saliva constituents. Unstimulated whole saliva samples were collected according to a standard protocol and concentrations of 8-hydroxy-2'-deoxyguanosine (8-OHdG), myeloperoxidase (MPO), malondialdehyde (MDA), total antioxidant capacity (TAC), and cortisol. The one-way ANOVA test was used to compare the levels of each oxidative stress biomarker between the three study groups and the hierarchical linear regression analysis was used to test the levels of salivary cortisol for prediction of other oxidative stress biomarkers. Significance levels were set at 95% confidence intervals and probability values ≤0.05.</p><p><strong>Results: </strong>8-OHdG was highest in WPS group (mean±SE 11,030.35±1829.16 pg/mL) while MDA and cortisol levels were highest in the cigarette smokers group (mean±SE 3.33±0.52 µM and 3.99±0.48 ng/mL, respectively) and MPO was highest in the control group (mean±SE 7.760±1.55 ng/mL). WPS group showed the highest TAC (mean±SE 0.3±0.03 mM). However, none of the tested makers reached a statistically significant difference.</p><p><strong>Conclusions: </strong>Despite subtle changes in some biomarkers, the salivary oxidative stress does not appear to be significantly influenced by smoking habits in periodontitis-free smokers.</p>","PeriodicalId":18709,"journal":{"name":"Minerva dental and oral science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141492627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Preservation of primary teeth in children is highly important. Pulpotomy is a commonly performed treatment procedure for primary teeth with extensive caries. Thus, biocompatibility of pulpotomy agents is highly important. Biodentine, calcium enriched mixture (CEM) cement, ferric sulfate, and mineral trioxide aggregate (MTA) Angelus are commonly used for this purpose. Thus, this study aimed to assess the apoptotic effects of Biodentine, CEM cement, ferric sulfate, and MTA on stem cells isolated from the human pulp of exfoliated deciduous teeth.
Methods: In this in-vitro, experimental study, stem cells isolated from the human pulp of exfoliated deciduous teeth were exposed to three different concentrations of Biodentine, CEM cement, ferric sulfate, and MTA for different time periods. The cytotoxicity of the materials was evaluated by flow cytometry using the annexin propidium iodide (PI) kit. Data were analyzed by ANOVA and Tukey's test at P<0.05 level of significance.
Results: All four tested materials induced significantly greater apoptosis compared with the control group. The difference in cell apoptosis caused by the first concentration of ferric sulfate and MTA was not significant at 24 hours. In other comparisons, the cytotoxicity of ferric sulfate was significantly lower than that of other materials. Biodentine showed higher cytotoxicity than MTA at first; but this difference faded over time. The cytotoxicity of CEM cement was comparable to that of MTA. The highest cell viability was noted at 24 hours in presence of the minimum concentration of ferric sulfate. The lowest cell viability was noted at 72 hours in presence of the maximum concentration of CEM cement.
Conclusions: In comparison with other materials, ferric sulfate showed minimum cytotoxicity; the cytotoxicity of the three cements was comparable. It appears that the concentration of ferric sulfate and the composition of cements are responsible for different levels of cytotoxicity.
背景:保护儿童的乳牙非常重要。牙髓切断术是对有大面积龋齿的基牙常用的治疗方法。因此,牙髓切断剂的生物相容性非常重要。常用的牙髓切断剂有生物正丁(Biodentine)、富钙混合物(CEM)水泥、硫酸铁和三氧化二铝矿物骨料(MTA)Angelus。因此,本研究旨在评估 Biodentine、CEM 水泥、硫酸铁和 MTA 对从脱落牙齿的人类牙髓中分离出来的干细胞的凋亡作用:在这项体外实验研究中,从脱落牙齿的人类牙髓中分离出来的干细胞在不同时间段内暴露于三种不同浓度的 Biodentine、CEM 水泥、硫酸铁和 MTA。使用附件素碘化丙啶(PI)试剂盒,通过流式细胞术评估这些材料的细胞毒性。结果采用方差分析和 Tukey's 检验进行数据分析:与对照组相比,所有四种测试材料都能诱导细胞凋亡。在 24 小时内,第一浓度硫酸铁和 MTA 引起的细胞凋亡差异不显著。在其他比较中,硫酸铁的细胞毒性明显低于其他材料。生物硫化碱的细胞毒性起初高于 MTA,但随着时间的推移,这种差异逐渐减弱。CEM 水泥的细胞毒性与 MTA 相当。在存在最低浓度硫酸铁的 24 小时内,细胞存活率最高。结论:与其他材料相比,硫酸铁的细胞毒性最小;三种水泥的细胞毒性相当。硫酸铁的浓度和水泥的成分似乎是造成不同程度细胞毒性的原因。
{"title":"Apoptotic effects of biodentine, calcium-enriched mixture (CEM) cement, ferric sulfate, and mineral trioxide aggregate (MTA) on human mesenchymal stem cells isolated from the human pulp of exfoliated deciduous teeth.","authors":"Bahareh Nazemi Salman, Mahshid Mohebbi Rad, Ehsan Saburi","doi":"10.23736/S2724-6329.24.04826-5","DOIUrl":"10.23736/S2724-6329.24.04826-5","url":null,"abstract":"<p><strong>Background: </strong>Preservation of primary teeth in children is highly important. Pulpotomy is a commonly performed treatment procedure for primary teeth with extensive caries. Thus, biocompatibility of pulpotomy agents is highly important. Biodentine, calcium enriched mixture (CEM) cement, ferric sulfate, and mineral trioxide aggregate (MTA) Angelus are commonly used for this purpose. Thus, this study aimed to assess the apoptotic effects of Biodentine, CEM cement, ferric sulfate, and MTA on stem cells isolated from the human pulp of exfoliated deciduous teeth.</p><p><strong>Methods: </strong>In this in-vitro, experimental study, stem cells isolated from the human pulp of exfoliated deciduous teeth were exposed to three different concentrations of Biodentine, CEM cement, ferric sulfate, and MTA for different time periods. The cytotoxicity of the materials was evaluated by flow cytometry using the annexin propidium iodide (PI) kit. Data were analyzed by ANOVA and Tukey's test at P<0.05 level of significance.</p><p><strong>Results: </strong>All four tested materials induced significantly greater apoptosis compared with the control group. The difference in cell apoptosis caused by the first concentration of ferric sulfate and MTA was not significant at 24 hours. In other comparisons, the cytotoxicity of ferric sulfate was significantly lower than that of other materials. Biodentine showed higher cytotoxicity than MTA at first; but this difference faded over time. The cytotoxicity of CEM cement was comparable to that of MTA. The highest cell viability was noted at 24 hours in presence of the minimum concentration of ferric sulfate. The lowest cell viability was noted at 72 hours in presence of the maximum concentration of CEM cement.</p><p><strong>Conclusions: </strong>In comparison with other materials, ferric sulfate showed minimum cytotoxicity; the cytotoxicity of the three cements was comparable. It appears that the concentration of ferric sulfate and the composition of cements are responsible for different levels of cytotoxicity.</p>","PeriodicalId":18709,"journal":{"name":"Minerva dental and oral science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141498384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-01Epub Date: 2024-07-02DOI: 10.23736/S2724-6329.24.04902-7
Ishwarya Sundaravadivelu, Kaviyarasi Renu, Sankaran Kavitha, Veeraraghavan V Priya, Rengasamy Gayathri, Vincenzo Ronsivalle, Marco Cicciù, Giuseppe Minervini
Background: Oral cancer is a serious health issue in both the developing and developed worlds, and it is one of the most common forms of cancer of the head and neck. In accordance with the 2017 World Health Organization classification, oral cancer can affect any part of the mouth, including the buccal mucosa, the front two-thirds of the tongue, the lip, the palate, the vestibule, the alveolus, the floor of the mouth, and the gingivae. Hematology and electrolyte balance have been proposed as tumor indicators and paths into cancer's genesis. Examining the patient's blood count and electrolyte levels in order to better understand their oral cancer.
Methods: Electrolyte abnormalities are common in cancer patients and may be caused by the disease itself or by treatment. Hyponatremia is the most frequent electrolyte problem in cancer patients, and it is typically caused by the syndrome of improper ADH secretion. Although electrolyte problems are associated with a worse prognosis for cancer patients, timely and effective therapy has the potential to enhance both short- and long-term results and quality of life. Hematological tests on patients with oral cancer, including differential cell count, white blood cell count, and hemoglobin level.
Results: Compared to healthy controls, oral cancer patients show statistically significant differences in a number of biochemical parameters, including electrolytes (sodium, P<0.05; potassium, P=0.89; chloride, P<0.05); differential count (neutrophils, P<0.05; basophils, P<0.05). A significant risk factor for cancer patients is an electrolyte imbalance, which has been linked to inappropriate anti-diabetic hormone release.
Conclusions: Clinicians will find these shifts in electrolytic level helpful in diagnosing and tracking oral cancer. Potentially malignant oral disorders and Oral squamous cell carcinoma may be better predicted using a combination of TLC, neutrophil, and lymphocyte counts, as shown in this study.
{"title":"Elucidating hematological profile and electrolyte balance in oral cancer patients.","authors":"Ishwarya Sundaravadivelu, Kaviyarasi Renu, Sankaran Kavitha, Veeraraghavan V Priya, Rengasamy Gayathri, Vincenzo Ronsivalle, Marco Cicciù, Giuseppe Minervini","doi":"10.23736/S2724-6329.24.04902-7","DOIUrl":"10.23736/S2724-6329.24.04902-7","url":null,"abstract":"<p><strong>Background: </strong>Oral cancer is a serious health issue in both the developing and developed worlds, and it is one of the most common forms of cancer of the head and neck. In accordance with the 2017 World Health Organization classification, oral cancer can affect any part of the mouth, including the buccal mucosa, the front two-thirds of the tongue, the lip, the palate, the vestibule, the alveolus, the floor of the mouth, and the gingivae. Hematology and electrolyte balance have been proposed as tumor indicators and paths into cancer's genesis. Examining the patient's blood count and electrolyte levels in order to better understand their oral cancer.</p><p><strong>Methods: </strong>Electrolyte abnormalities are common in cancer patients and may be caused by the disease itself or by treatment. Hyponatremia is the most frequent electrolyte problem in cancer patients, and it is typically caused by the syndrome of improper ADH secretion. Although electrolyte problems are associated with a worse prognosis for cancer patients, timely and effective therapy has the potential to enhance both short- and long-term results and quality of life. Hematological tests on patients with oral cancer, including differential cell count, white blood cell count, and hemoglobin level.</p><p><strong>Results: </strong>Compared to healthy controls, oral cancer patients show statistically significant differences in a number of biochemical parameters, including electrolytes (sodium, P<0.05; potassium, P=0.89; chloride, P<0.05); differential count (neutrophils, P<0.05; basophils, P<0.05). A significant risk factor for cancer patients is an electrolyte imbalance, which has been linked to inappropriate anti-diabetic hormone release.</p><p><strong>Conclusions: </strong>Clinicians will find these shifts in electrolytic level helpful in diagnosing and tracking oral cancer. Potentially malignant oral disorders and Oral squamous cell carcinoma may be better predicted using a combination of TLC, neutrophil, and lymphocyte counts, as shown in this study.</p>","PeriodicalId":18709,"journal":{"name":"Minerva dental and oral science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141492626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-01Epub Date: 2024-07-04DOI: 10.23736/S2724-6329.24.04854-X
Guilherme J Lopes de Oliveira, Jackeline do Nascimento Tsurumaki, Mauricio A Aroni, Elcio Marcantonio, Rosemary A Marcantonio
Background: The aim of this study was to evaluate the effect of the avocado/soybean unsaponifiables (ASU) in the treatment of induced periodontitis in rats with experimental arthritis.
Methods: Sixty rats were randomly assigned to 4 groups according to the type of treatment and the systemic condition of the animals: CTR-S: healthy animals in which saline solution (SS) was administered; ASU-S: healthy animals in which ASU (0.6 mg/kg) was administered; AR/ASU-S: animals with induced arthritis in which ASU was administered; AR-S: animals with induced arthritis in which SS was administered. Periodontitis was induced by ligatures, maintained for 15 days. Subsequently, the treatment was performed by scaling with hand instruments. The SS and ASU were administered daily by gavage until euthanasia of the animals that occurred at 7, 15 or 30 days after the scaling procedure (N.=5 animals/group). Bone resorption, inflammatory infiltrate composition, and osteoclastogenesis were assessed.
Results: The AR-S group had greater bone loss, smaller amounts of fibroblasts and larger amounts of inflammatory cells than all other groups. In addition, the AR-S group had greater osteoclastogenesis in relation to the healthy animal groups.
Conclusions: The use of ASU improved the healing pattern after treatment for experimental periodontitis in animals with arthritis reducing the periodontal bone loss.
{"title":"Periodontal host-modulation therapy with avocado/soybean unsaponifiables in rats with arthritis.","authors":"Guilherme J Lopes de Oliveira, Jackeline do Nascimento Tsurumaki, Mauricio A Aroni, Elcio Marcantonio, Rosemary A Marcantonio","doi":"10.23736/S2724-6329.24.04854-X","DOIUrl":"10.23736/S2724-6329.24.04854-X","url":null,"abstract":"<p><strong>Background: </strong>The aim of this study was to evaluate the effect of the avocado/soybean unsaponifiables (ASU) in the treatment of induced periodontitis in rats with experimental arthritis.</p><p><strong>Methods: </strong>Sixty rats were randomly assigned to 4 groups according to the type of treatment and the systemic condition of the animals: CTR-S: healthy animals in which saline solution (SS) was administered; ASU-S: healthy animals in which ASU (0.6 mg/kg) was administered; AR/ASU-S: animals with induced arthritis in which ASU was administered; AR-S: animals with induced arthritis in which SS was administered. Periodontitis was induced by ligatures, maintained for 15 days. Subsequently, the treatment was performed by scaling with hand instruments. The SS and ASU were administered daily by gavage until euthanasia of the animals that occurred at 7, 15 or 30 days after the scaling procedure (N.=5 animals/group). Bone resorption, inflammatory infiltrate composition, and osteoclastogenesis were assessed.</p><p><strong>Results: </strong>The AR-S group had greater bone loss, smaller amounts of fibroblasts and larger amounts of inflammatory cells than all other groups. In addition, the AR-S group had greater osteoclastogenesis in relation to the healthy animal groups.</p><p><strong>Conclusions: </strong>The use of ASU improved the healing pattern after treatment for experimental periodontitis in animals with arthritis reducing the periodontal bone loss.</p>","PeriodicalId":18709,"journal":{"name":"Minerva dental and oral science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141498441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-01Epub Date: 2024-03-18DOI: 10.23736/S2724-6329.23.04904-5
Alessandro Quaranta, Wang L Hui
{"title":"L.O.V.E.R.R.S.: a conceptual framework to shape your purpose and a life of fulfilment.","authors":"Alessandro Quaranta, Wang L Hui","doi":"10.23736/S2724-6329.23.04904-5","DOIUrl":"10.23736/S2724-6329.23.04904-5","url":null,"abstract":"","PeriodicalId":18709,"journal":{"name":"Minerva dental and oral science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140158478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-15DOI: 10.23736/S2724-6329.23.04865-9
Mahesh Shenoy K, Talal M Alkhelaiwi, Abdullah H Alasiri, Badr R Hejazi, Khaled R Alkathiri, Shahzeb H Ansari, Diana Russo, Vincenzo Ronsivalle, Marco Cicciù, Giuseppe Minervini
Background: According to Centers for Medicare and Medicaid Services HHS 2017, when a medical disease suddenly manifests itself with acute symptoms of sufficient intensity (including extreme pain) endangering the patient's health, seriously impairing body functions or bringing an organ or its portion to be seriously dysfunctional, a medical emergency has occurred. Therefore, this study aimed to assess the knowledge, attitude, and perceived confidence in handling medical emergencies among dental students by the means of conducting this investigation.
Methods: This is a cross-sectional study conducted among the different hierarchies of dentists of Riyadh using an online survey; 387 dentists from varying clinical levels (9 to 12) were included in this study. The questionnaire used for this study consisted of questions related to demographic data followed by questions including knowledge, attitude, and confidence towards handling medical emergencies in clinics.
Results: In this study, male participants represented 64.4% and female subjects represented 35.6% of the total population. Most of the undergraduates were in their 5th year of dentistry school and accounted for 36.9%, followed by 6th year 36.2% and 4th year 26.8%. 67.1% of the undergraduates never encountered any medical emergency during their study, while 32.9% encountered an emergency. Furthermore, 58.4% of the analyzed subjects recorded medical history thoroughly, while priority in an emergency was adrenaline, with a percentage of 45%. For the patient suffering syncope the Trendelenburg position was chosen. Also, the first action choice with unresponsive patients was CPR.
Conclusions: In the present study, the findings revealed that the majority of the population never encountered an emergency in their career, but their knowledge about drug priority and patient's position was good. Most of them do not measure vital signs routinely; specifically, females do not usually measure, while males measure when needed. About Basic Life Support (BLS), participants took BLS or were interested in taking it in the future; furthermore, in group population, both have taken it and are interested in further carrying it.
{"title":"The knowledge, attitude and perceived confidence in handling medical emergencies among dental students.","authors":"Mahesh Shenoy K, Talal M Alkhelaiwi, Abdullah H Alasiri, Badr R Hejazi, Khaled R Alkathiri, Shahzeb H Ansari, Diana Russo, Vincenzo Ronsivalle, Marco Cicciù, Giuseppe Minervini","doi":"10.23736/S2724-6329.23.04865-9","DOIUrl":"https://doi.org/10.23736/S2724-6329.23.04865-9","url":null,"abstract":"<p><strong>Background: </strong>According to Centers for Medicare and Medicaid Services HHS 2017, when a medical disease suddenly manifests itself with acute symptoms of sufficient intensity (including extreme pain) endangering the patient's health, seriously impairing body functions or bringing an organ or its portion to be seriously dysfunctional, a medical emergency has occurred. Therefore, this study aimed to assess the knowledge, attitude, and perceived confidence in handling medical emergencies among dental students by the means of conducting this investigation.</p><p><strong>Methods: </strong>This is a cross-sectional study conducted among the different hierarchies of dentists of Riyadh using an online survey; 387 dentists from varying clinical levels (9 to 12) were included in this study. The questionnaire used for this study consisted of questions related to demographic data followed by questions including knowledge, attitude, and confidence towards handling medical emergencies in clinics.</p><p><strong>Results: </strong>In this study, male participants represented 64.4% and female subjects represented 35.6% of the total population. Most of the undergraduates were in their 5<sup>th</sup> year of dentistry school and accounted for 36.9%, followed by 6<sup>th</sup> year 36.2% and 4<sup>th</sup> year 26.8%. 67.1% of the undergraduates never encountered any medical emergency during their study, while 32.9% encountered an emergency. Furthermore, 58.4% of the analyzed subjects recorded medical history thoroughly, while priority in an emergency was adrenaline, with a percentage of 45%. For the patient suffering syncope the Trendelenburg position was chosen. Also, the first action choice with unresponsive patients was CPR.</p><p><strong>Conclusions: </strong>In the present study, the findings revealed that the majority of the population never encountered an emergency in their career, but their knowledge about drug priority and patient's position was good. Most of them do not measure vital signs routinely; specifically, females do not usually measure, while males measure when needed. About Basic Life Support (BLS), participants took BLS or were interested in taking it in the future; furthermore, in group population, both have taken it and are interested in further carrying it.</p>","PeriodicalId":18709,"journal":{"name":"Minerva dental and oral science","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141616853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}