Pub Date : 2023-01-01DOI: 10.1080/21501203.2022.2155261
Nivisti Singh, Bruce Sithole, Roshini Govinden
The search for a novel microbial producer of cellulases including a glucose tolerant β-glucosidase is a challenge as most are inhibited by their product glucose. This study aims to screen for cellulolytic fungi using qualitative and quantitative screening methods. Primary screening revealed 34 of 46 fungal isolates with β-glucosidase activity. Eleven and 13 of these also displayed endoglucanase and exoglucanase activities, respectively. During secondary screening, this number was reduced to 26 β-glucosidase producers with 13 also having endoglucanase and exoglucanase activities. Isolate C1 displayed enhanced production of β-glucosidases in the presence of 0.05 M glucose (69% higher activity). Optimisation of growth conditions for β-glucosidase production by one variable at a time experiments improved production for (isolates) PS1 (64%), MB5 (84%), and C2 (69%). Isolate PS1 identified as Chaetomella sp. BBA70074 displayed the highest tolerance to glucose, retaining 10% of β-glucosidase activity in the presence of 0.8 M glucose. Tolerance to glucose increased to 14% when produced under optimal conditions. β-Glucosidase had a molecular weight of 170 kDa with a pH and temperature optima of 6 and 70°C, respectively. Future studies will include optimisation of the production of the glucose tolerant enzyme by Chaetomella sp. BBA70074.
{"title":"Screening for cellulases and preliminary optimisation of glucose tolerant β-glucosidase production and characterisation.","authors":"Nivisti Singh, Bruce Sithole, Roshini Govinden","doi":"10.1080/21501203.2022.2155261","DOIUrl":"https://doi.org/10.1080/21501203.2022.2155261","url":null,"abstract":"<p><p>The search for a novel microbial producer of cellulases including a glucose tolerant β-glucosidase is a challenge as most are inhibited by their product glucose. This study aims to screen for cellulolytic fungi using qualitative and quantitative screening methods. Primary screening revealed 34 of 46 fungal isolates with β-glucosidase activity. Eleven and 13 of these also displayed endoglucanase and exoglucanase activities, respectively. During secondary screening, this number was reduced to 26 β-glucosidase producers with 13 also having endoglucanase and exoglucanase activities. Isolate C1 displayed enhanced production of β-glucosidases in the presence of 0.05 M glucose (69% higher activity). Optimisation of growth conditions for β-glucosidase production by one variable at a time experiments improved production for (isolates) PS1 (64%), MB5 (84%), and C2 (69%). Isolate PS1 identified as <i>Chaetomella</i> sp. BBA70074 displayed the highest tolerance to glucose, retaining 10% of β-glucosidase activity in the presence of 0.8 M glucose. Tolerance to glucose increased to 14% when produced under optimal conditions. β-Glucosidase had a molecular weight of 170 kDa with a pH and temperature optima of 6 and 70°C, respectively. Future studies will include optimisation of the production of the glucose tolerant enzyme by <i>Chaetomella</i> sp. BBA70074.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10161942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9485713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.1080/21501203.2022.2096144
Meng Pan, Lu Lin, Chengming Tian, Xinlei Fan
Golden rain trees (Koelreuteria paniculata) are largely cultivated because of their important ornamental, medicinal, and economic value. However, they are affected by canker and dieback disease to a large extent. To determine the fungi associated with canker and dieback disease of golden rain trees, isolations were obtained from diseased branches and twigs during 2019 and 2020 in greenbelts and nurseries in Beijing, China. Isolates were identified as six species (Allocryptovalsa castaneicola, Botryosphaeria dothidea, Cytospora koelreutericola sp. nov., Dothiorella acericola, Eutypella citricola, and Peroneutypa scoparia) based on morphological features and phylogenetic analyses of ITS, act, rpb2, tef1-α, and tub2. The results of pathogenicity tests indicated that all fungi produced discoloration and Botryosphaeria dothidea was highly aggressive to golden rain tree. In conclusion, this study explored the taxonomy, phylogeny, and pathogenicity of different fungal species associated with canker and dieback disease on golden rain tree and provided fundamental knowledge to improve disease management.
{"title":"Identification and pathogenicity of six fungal species causing canker and dieback disease on golden rain tree in Beijing, China.","authors":"Meng Pan, Lu Lin, Chengming Tian, Xinlei Fan","doi":"10.1080/21501203.2022.2096144","DOIUrl":"https://doi.org/10.1080/21501203.2022.2096144","url":null,"abstract":"<p><p>Golden rain trees (<i>Koelreuteria paniculata</i>) are largely cultivated because of their important ornamental, medicinal, and economic value. However, they are affected by canker and dieback disease to a large extent. To determine the fungi associated with canker and dieback disease of golden rain trees, isolations were obtained from diseased branches and twigs during 2019 and 2020 in greenbelts and nurseries in Beijing, China. Isolates were identified as six species (<i>Allocryptovalsa castaneicola, Botryosphaeria dothidea, Cytospora koelreutericola</i> sp. nov., <i>Dothiorella acericola, Eutypella citricola</i>, and <i>Peroneutypa scoparia</i>) based on morphological features and phylogenetic analyses of ITS, <i>act, rpb2, tef1-α</i>, and <i>tub2</i>. The results of pathogenicity tests indicated that all fungi produced discoloration and <i>Botryosphaeria dothidea</i> was highly aggressive to golden rain tree. In conclusion, this study explored the taxonomy, phylogeny, and pathogenicity of different fungal species associated with canker and dieback disease on golden rain tree and provided fundamental knowledge to improve disease management.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9930857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9329374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The genus Armillaria has high edible and medical values, with zones of antagonism often occurring when different species are paired in culture on agar media, while the antagonism-induced metabolic alteration remains unclear. Here, the metabolome of mycelial exudates of two Chinese Armillaria biological species, C and G, co-cultured or cultured separately was analysed to discover the candidate biomarkers and the key metabolic pathways involved in Armillaria antagonists. A total of 2,377 metabolites were identified, mainly organic acids and derivatives, lipids and lipid-like molecules, and organoheterocyclic compounds. There were 248 and 142 differentially expressed metabolites between group C-G and C, C-G, and G, respectively, and fourteen common differentially expressed metabolites including malate, uracil, Leu-Gln-Arg, etc. Metabolic pathways like TCA cycle and pyrimidine metabolism were significantly affected by C-G co-culture. Additionally, 156 new metabolites (largely organic acids and derivatives) including 32 potential antifungal compounds, primarily enriched into biosynthesis of secondary metabolites pathways were identified in C-G co-culture mode. We concluded that malate and uracil could be used as the candidate biomarkers, and TCA cycle and pyrimidine metabolism were the key metabolic pathways involved in Armillaria antagonists. The metabolic changes revealed in this study provide insights into the mechanisms underlying fungal antagonists.
{"title":"Metabolomics analysis of mycelial exudates provides insights into fungal antagonists of <i>Armillaria</i>.","authors":"Jian Zhan, Jing Yuan, Jianwei Liu, Fengming Zhang, Fuqiang Yu, Yanliang Wang","doi":"10.1080/21501203.2023.2238753","DOIUrl":"https://doi.org/10.1080/21501203.2023.2238753","url":null,"abstract":"<p><p>The genus <i>Armillaria</i> has high edible and medical values, with zones of antagonism often occurring when different species are paired in culture on agar media, while the antagonism-induced metabolic alteration remains unclear. Here, the metabolome of mycelial exudates of two Chinese <i>Armillaria</i> biological species, C and G, co-cultured or cultured separately was analysed to discover the candidate biomarkers and the key metabolic pathways involved in <i>Armillaria</i> antagonists. A total of 2,377 metabolites were identified, mainly organic acids and derivatives, lipids and lipid-like molecules, and organoheterocyclic compounds. There were 248 and 142 differentially expressed metabolites between group C-G and C, C-G, and G, respectively, and fourteen common differentially expressed metabolites including malate, uracil, Leu-Gln-Arg, etc. Metabolic pathways like TCA cycle and pyrimidine metabolism were significantly affected by C-G co-culture. Additionally, 156 new metabolites (largely organic acids and derivatives) including 32 potential antifungal compounds, primarily enriched into biosynthesis of secondary metabolites pathways were identified in C-G co-culture mode. We concluded that malate and uracil could be used as the candidate biomarkers, and TCA cycle and pyrimidine metabolism were the key metabolic pathways involved in <i>Armillaria</i> antagonists. The metabolic changes revealed in this study provide insights into the mechanisms underlying fungal antagonists.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/02/ff/TMYC_14_2238753.PMC10424624.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10547148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-28eCollection Date: 2022-01-01DOI: 10.1080/21501203.2022.2138599
Venkatramanan Mahendrarajan, Vinay Kumar Bari
Candida albicans and its related species can cause opportunistic infections such as "candidiasis" in immunocompromised individuals with a high morbidity and mortality rate. Several antifungal drugs available in the market are often used to treat infections caused by pathogenic fungi. However, in fungi, the development of resistance against these drugs quickly evolved. Candida is a dimorphic fungus that can switch between yeast to hyphae form, requires an active biosynthesis of membrane constituents. Sphingolipid and ergosterol molecules, are the major fungal plasma membrane components, and their interaction with the antifungal drug can modulate drug susceptibility. A lipophilic compound farnesol acts as a quorum-sensing molecule synthesised by the isoprenoid biosynthesis pathway in the fungal pathogen Candida. Farnesol is secreted in a cell density-dependent manner inhibits hyphae germination and biofilm formation. In this study, we have investigated whether the farnesol molecules affect the drug susceptibility of the antifungal drug Amphotericin B (AmB) which mainly binds with ergosterol, and Aureobasidin A (AbA), a complex sphingolipid biosynthesis inhibitor. Our studies revealed that a non-toxic and low concentration of farnesol can reduce the efficacy of AmB and AbA on yeast cells. This reduction is probably through the alteration in the complex sphingolipid biosynthesis and ATP-binding cassette (ABC) type membrane transport activity. These findings may shed light on a new direction to explore the role of lipid molecules in the antifungal drug resistance mechanisms in pathogenic yeast.
白色念珠菌及其相关菌种可在免疫功能低下的个体中引起机会性感染,如“念珠菌病”,发病率和死亡率高。市场上几种抗真菌药物通常用于治疗由致病性真菌引起的感染。然而,真菌对这些药物的耐药性发展很快。念珠菌是一种二态真菌,可以在酵母和菌丝形态之间转换,需要活性的生物合成膜成分。鞘脂和麦角甾醇分子是真菌质膜的主要成分,它们与抗真菌药物的相互作用可以调节药物的敏感性。一种亲脂化合物法尼醇作为群体感应分子,在真菌病原体念珠菌中通过类异戊二烯生物合成途径合成。法尼醇以细胞密度依赖的方式分泌,抑制菌丝萌发和生物膜的形成。在这项研究中,我们研究了法尼醇分子是否影响抗真菌药物两性霉素B (AmB)的药物敏感性,两性霉素B主要与麦角甾醇结合,而Aureobasidin A (AbA)是一种复杂的鞘脂生物合成抑制剂。我们的研究表明,无毒和低浓度的法尼醇可以降低AmB和AbA对酵母细胞的作用。这种减少可能是由于复杂鞘脂生物合成和atp结合盒(ABC)型膜运输活性的改变。这些发现可能为探索脂质分子在病原菌抗真菌耐药机制中的作用提供了新的方向。
{"title":"A critical role of farnesol in the modulation of Amphotericin B and Aureobasidin A antifungal drug susceptibility.","authors":"Venkatramanan Mahendrarajan, Vinay Kumar Bari","doi":"10.1080/21501203.2022.2138599","DOIUrl":"https://doi.org/10.1080/21501203.2022.2138599","url":null,"abstract":"<p><p><i>Candida albicans</i> and its related species can cause opportunistic infections such as \"candidiasis\" in immunocompromised individuals with a high morbidity and mortality rate. Several antifungal drugs available in the market are often used to treat infections caused by pathogenic fungi. However, in fungi, the development of resistance against these drugs quickly evolved. <i>Candida</i> is a dimorphic fungus that can switch between yeast to hyphae form, requires an active biosynthesis of membrane constituents. Sphingolipid and ergosterol molecules, are the major fungal plasma membrane components, and their interaction with the antifungal drug can modulate drug susceptibility. A lipophilic compound farnesol acts as a quorum-sensing molecule synthesised by the isoprenoid biosynthesis pathway in the fungal pathogen <i>Candida</i>. Farnesol is secreted in a cell density-dependent manner inhibits hyphae germination and biofilm formation. In this study, we have investigated whether the farnesol molecules affect the drug susceptibility of the antifungal drug Amphotericin B (AmB) which mainly binds with ergosterol, and Aureobasidin A (AbA), a complex sphingolipid biosynthesis inhibitor. Our studies revealed that a non-toxic and low concentration of farnesol can reduce the efficacy of AmB and AbA on yeast cells. This reduction is probably through the alteration in the complex sphingolipid biosynthesis and ATP-binding cassette (ABC) type membrane transport activity. These findings may shed light on a new direction to explore the role of lipid molecules in the antifungal drug resistance mechanisms in pathogenic yeast.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2022-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40485814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-27eCollection Date: 2022-01-01DOI: 10.1080/21501203.2022.2104950
Paris Chakravarty
Fungal contamination in water-damaged houses has become a major concern because of their potential health effects. During our survey in 100 water-damaged houses in southern California, we have reported 42 outdoor and 14 indoor fungal species throughout the year. Six commonly occurring indoor fungal species are Alternaria alternata, Aspergillus niger, Chaetomium globosum, Cladosporium herbarum, Penicillium chrysogenum and Stachybotrys chartarum. In the damp building materials, S. chartarum was found to be associated with A. niger, C. globosum and P. chrysogenum but not with A. alternata and C. herbarum. Stachybotrys chartarum showed strong antagonistic effect against A. alternata and C. herbarum and significantly inhibited in vitro growth of A. alternata and C. herbarum but had no effect on A. niger, C. globosum and P. chrysogenum. Two trichothecenes, produced by Stachybotrys sp., trichodermin and trichodermol, significantly inhibited spore germination and in vitro growth of A. alternata and C. herbarum but had no effect on A. niger, C. globosum, P. chrysogenum and S. chartarum. In the damp building materials (drywall, ceiling tile, and oak wood), S. chartarum significantly inhibited the growth of A. alternata and C. herbarum and had no effect on A. niger, C. globosum and P. chrysogenum in these substrata.
{"title":"Mycobiota and mycotoxin-producing fungi in southern California: their colonisation and <i>in vitro</i> interactions.","authors":"Paris Chakravarty","doi":"10.1080/21501203.2022.2104950","DOIUrl":"https://doi.org/10.1080/21501203.2022.2104950","url":null,"abstract":"<p><p>Fungal contamination in water-damaged houses has become a major concern because of their potential health effects. During our survey in 100 water-damaged houses in southern California, we have reported 42 outdoor and 14 indoor fungal species throughout the year. Six commonly occurring indoor fungal species are <i>Alternaria alternata, Aspergillus niger, Chaetomium globosum, Cladosporium herbarum, Penicillium chrysogenum</i> and <i>Stachybotrys chartarum</i>. In the damp building materials, <i>S. chartarum</i> was found to be associated with <i>A. niger, C. globosum</i> and <i>P. chrysogenum</i> but not with <i>A. alternata</i> and <i>C. herbarum. Stachybotrys chartarum</i> showed strong antagonistic effect against <i>A. alternata</i> and <i>C. herbarum</i> and significantly inhibited <i>in vitro</i> growth of <i>A. alternata</i> and <i>C. herbarum</i> but had no effect on <i>A. niger, C. globosum</i> and <i>P. chrysogenum</i>. Two trichothecenes, produced by <i>Stachybotrys</i> sp., trichodermin and trichodermol, significantly inhibited spore germination and <i>in vitro</i> growth of <i>A. alternata</i> and <i>C. herbarum</i> but had no effect on <i>A. niger, C. globosum, P. chrysogenum</i> and <i>S. chartarum</i>. In the damp building materials (drywall, ceiling tile, and oak wood), <i>S. chartarum</i> significantly inhibited the growth of <i>A. alternata</i> and <i>C. herbarum</i> and had no effect on <i>A. niger, C. globosum and P. chrysogenum</i> in these substrata.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2022-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40485810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-05eCollection Date: 2022-01-01DOI: 10.1080/21501203.2022.2094012
Ming Zhang, De-Chun Xie, Chao-Qun Wang, Wang Qiu Deng, Tai-Hui Li
Species of Gyroporus from southern China were studied in this study. Based on morphology and molecular phylogenetic analyses of DNA sequences from the nuclear ribosomal internal transcribed spacer (ITS), the nuclear ribosomal large subunit (nrLSU), and the mitochondrial adenosine triphosphate ATP synthase subunit 6 (atp6), Gyroporus was divided into four main branches in the phylogenetic tree, and four sections were firstly proposed i.e. Gyroporus sect. Castaneus, G. sect. Cyanescens, G. sect. Longicystidiatus and G. sect. Pallidus. Five new species, i.e. G. alboluteus, G. atrocyanescens, G. pseudolongicystidiatus, G. pallidus and G. subcaerulescens, were revealed from China, and their phylogenetic positions were also analysed. Among them, G. alboluteus and G. pallidus were nested into the sect. Pallidus, although morphologically similar to G. castaneus; G. atrocyanescens and G. subcaerulescens, with obvious cyanescent oxidation reactions, were nested into the sect. Cyanescens; and G. pseudolongicystidiatus characterised by its long cystidia and was nested into the sect. Longicystidiatus. The new species were formally described and illustrated in the present study, and a key to the sections and species of Gyroporus in China was provided.
{"title":"New insights into the genus <i>Gyroporus</i> (Gyroporaceae, Boletales), with establishment of four new sections and description of five new species from China.","authors":"Ming Zhang, De-Chun Xie, Chao-Qun Wang, Wang Qiu Deng, Tai-Hui Li","doi":"10.1080/21501203.2022.2094012","DOIUrl":"10.1080/21501203.2022.2094012","url":null,"abstract":"<p><p>Species of <i>Gyroporus</i> from southern China were studied in this study. Based on morphology and molecular phylogenetic analyses of DNA sequences from the nuclear ribosomal internal transcribed spacer (ITS), the nuclear ribosomal large subunit (nrLSU), and the mitochondrial adenosine triphosphate ATP synthase subunit 6 (<i>atp6), Gyroporus</i> was divided into four main branches in the phylogenetic tree, and four sections were firstly proposed i.e. <i>Gyroporus</i> sect. <i>Castaneus, G</i>. sect. <i>Cyanescens, G</i>. sect. <i>Longicystidiatus</i> and <i>G</i>. sect. <i>Pallidus</i>. Five new species, i.e. <i>G. alboluteus, G. atrocyanescens, G. pseudolongicystidiatus, G. pallidus</i> and <i>G. subcaerulescens</i>, were revealed from China, and their phylogenetic positions were also analysed. Among them, <i>G. alboluteus</i> and <i>G. pallidus</i> were nested into the sect. <i>Pallidus</i>, although morphologically similar to <i>G. castaneus; G. atrocyanescens</i> and <i>G. subcaerulescens</i>, with obvious cyanescent oxidation reactions, were nested into the sect. <i>Cyanescens</i>; and <i>G. pseudolongicystidiatus</i> characterised by its long cystidia and was nested into the sect. <i>Longicystidiatus</i>. The new species were formally described and illustrated in the present study, and a key to the sections and species of <i>Gyroporus</i> in China was provided.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2022-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40609163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-20eCollection Date: 2022-01-01DOI: 10.1080/21501203.2022.2079745
Priyashini Dhaver, Brett Pletschke, Bruce Sithole, Roshini Govinden
Fungi are renowned for their ability to produce extracellular enzymes into their surrounding environment. Xylanases are hydrolytic enzymes capable of xylan degradation. The objectives of this study were to isolate, screen for potential xylanolytic fungi from soil and tree bark samples from three locations in South Africa and to determine their growth conditions for maximum xylanase production. Forty-six isolates were obtained based on clearing zone formation on xylan-enriched agar plates using Congo red indicator. Xylanase activity was quantified during submerged fermentation. Isolate MS5, identified as Trichoderma harzianum with the highest enzyme activity (38.17 U/ml) was selected for further studies based on thermophilic properties (70°C) and pH (5.0). The culture conditions; incubation period (5 days), agitation speed (160 rpm) wheat bran (1%) and ammonium sulphate (1.2%) were optimised further. Biochemical characterisation of the crude enzyme revealed two pH and temperature optima (pH 6.0 at 60°C and 70°C, pH 8.0 at 55°C and 75°C). The enzyme retained >70% activity after 4 h at pH 6.0 at 70°C. SDS-PAGE revealed multiple protein bands with a prominent band at 70 kDa. Substrate Native PAGE revealed multiple isoforms between 55 and 130 kDa. This enzyme will be beneficial for applications in the animal feed and biofuels industries.
{"title":"Isolation, screening, preliminary optimisation and characterisation of thermostable xylanase production under submerged fermentation by fungi in Durban, South Africa.","authors":"Priyashini Dhaver, Brett Pletschke, Bruce Sithole, Roshini Govinden","doi":"10.1080/21501203.2022.2079745","DOIUrl":"https://doi.org/10.1080/21501203.2022.2079745","url":null,"abstract":"<p><p>Fungi are renowned for their ability to produce extracellular enzymes into their surrounding environment. Xylanases are hydrolytic enzymes capable of xylan degradation. The objectives of this study were to isolate, screen for potential xylanolytic fungi from soil and tree bark samples from three locations in South Africa and to determine their growth conditions for maximum xylanase production. Forty-six isolates were obtained based on clearing zone formation on xylan-enriched agar plates using Congo red indicator. Xylanase activity was quantified during submerged fermentation. Isolate MS5, identified as <i>Trichoderma harzianum</i> with the highest enzyme activity (38.17 U/ml) was selected for further studies based on thermophilic properties (70°C) and pH (5.0). The culture conditions; incubation period (5 days), agitation speed (160 rpm) wheat bran (1%) and ammonium sulphate (1.2%) were optimised further. Biochemical characterisation of the crude enzyme revealed two pH and temperature optima (pH 6.0 at 60°C and 70°C, pH 8.0 at 55°C and 75°C). The enzyme retained >70% activity after 4 h at pH 6.0 at 70°C. SDS-PAGE revealed multiple protein bands with a prominent band at 70 kDa. Substrate Native PAGE revealed multiple isoforms between 55 and 130 kDa. This enzyme will be beneficial for applications in the animal feed and biofuels industries.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2022-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673795/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40485811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-20eCollection Date: 2022-01-01DOI: 10.1080/21501203.2022.2089755
Viktor Papp, Yu-Cheng Dai
In recent years, the scientific names of many cultivated and well-known medicinal fungal species have been changed. However, the results of taxonomic and nomenclature works on these economically important fungi are often overlooked or ignored in applied researches. The incorrect use of scientific names may cause uncertainty in research and in the global medicinal mushroom market. In this paper, we briefly review the current taxonomy and nomenclature of "Fuling" medicinal mushroom and make a proposal for biochemists, pharmacists and businessmen on the correct use of scientific names related to this species. Based on the recent taxonomic results and nomenclatural proposals, the use of the names Wolfiporia extensa, W. cocos and especially Poria cocos for the "Fuling" mushroom are incorrect and misleading; therefore, the acceptance of the names Pachyma hoelen or Wolfiporia hoelen is recommended.
近年来,许多栽培和知名的药用真菌物种的学名发生了变化。然而,对这些具有重要经济意义的真菌的分类和命名工作的结果在应用研究中往往被忽视或忽视。学名的不正确使用可能会导致研究和全球药用蘑菇市场的不确定性。本文简要回顾了“涪陵”药用菌的分类和命名现状,并对生化学家、药剂师和商人正确使用“涪陵”药用菌的学名提出了建议。根据最近的分类结果和命名建议,将“涪陵”蘑菇命名为Wolfiporia extensa, W. cocos,特别是Poria cocos是不正确的,具有误导性;因此,建议接受Pachyma hoelen或Wolfiporia hoelen的名称。
{"title":"What is the correct scientific name for \"Fuling\" medicinal mushroom?","authors":"Viktor Papp, Yu-Cheng Dai","doi":"10.1080/21501203.2022.2089755","DOIUrl":"https://doi.org/10.1080/21501203.2022.2089755","url":null,"abstract":"<p><p>In recent years, the scientific names of many cultivated and well-known medicinal fungal species have been changed. However, the results of taxonomic and nomenclature works on these economically important fungi are often overlooked or ignored in applied researches. The incorrect use of scientific names may cause uncertainty in research and in the global medicinal mushroom market. In this paper, we briefly review the current taxonomy and nomenclature of \"Fuling\" medicinal mushroom and make a proposal for biochemists, pharmacists and businessmen on the correct use of scientific names related to this species. Based on the recent taxonomic results and nomenclatural proposals, the use of the names <i>Wolfiporia extensa, W. cocos</i> and especially <i>Poria cocos</i> for the \"Fuling\" mushroom are incorrect and misleading; therefore, the acceptance of the names <i>Pachyma hoelen</i> or <i>Wolfiporia hoelen</i> is recommended.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":null,"pages":null},"PeriodicalIF":4.2,"publicationDate":"2022-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354628/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40609164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Polysaccharides in boletes (Boletales) are economically significant to both function food and medicinal industries. The polysaccharides were extracted from the fruit bodies of eight boletes, namely, Aureoboletus longicollis, Butyriboletus hainanensis, Crocinoboletus rufoaureus, Hemioporus japonicus, Neoboletus infuscatus, Neoboletus obscureumbrinus, Tylopilus otsuensis, Xanthoconium fusciceps, which were collected from tropical China; their physicochemical properties and antioxidant activities were characterised and evaluated, respectively. The results revealed that the polysaccharides among the eight boletes were mainly composed of glucose, mannose, and galactose, with a broad molecular weight range, and contained a pyranose ring revealed by FT-IR and NMR spectral analyses. Many factors such as different species of boletes, geographic conditions, molecular weight, configuration, and monosaccharide content may affect the antioxidant power of polysaccharides, simultaneously, instead of one single factor. The antioxidant activities of the polysaccharides were measured according to in vitro assays of DPPH scavenging, superoxide anion scavenging, and ferrous ion reducing tests. The polysaccharide of C. rufoaureus has greatly superior antioxidant activity and it could serve as potential functional food or medicine.
{"title":"Preparation, structural characterisation, and antioxidant activities of polysaccharides from eight boletes (Boletales) in tropical China.","authors":"Run Tian, Hui Chai, Jun-Qiang Qiu, Zhi-Qun Liang, Hui-Jing Xie, Yong Wang, Nian-Kai Zeng","doi":"10.1080/21501203.2022.2069172","DOIUrl":"10.1080/21501203.2022.2069172","url":null,"abstract":"<p><p>Polysaccharides in boletes (Boletales) are economically significant to both function food and medicinal industries. The polysaccharides were extracted from the fruit bodies of eight boletes, namely, <i>Aureoboletus longicollis, Butyriboletus hainanensis, Crocinoboletus rufoaureus, Hemioporus japonicus, Neoboletus infuscatus, Neoboletus obscureumbrinus, Tylopilus otsuensis, Xanthoconium fusciceps</i>, which were collected from tropical China; their physicochemical properties and antioxidant activities were characterised and evaluated, respectively. The results revealed that the polysaccharides among the eight boletes were mainly composed of glucose, mannose, and galactose, with a broad molecular weight range, and contained a pyranose ring revealed by FT-IR and NMR spectral analyses. Many factors such as different species of boletes, geographic conditions, molecular weight, configuration, and monosaccharide content may affect the antioxidant power of polysaccharides, simultaneously, instead of one single factor. The antioxidant activities of the polysaccharides were measured according to <i>in vitro</i> assays of DPPH scavenging, superoxide anion scavenging, and ferrous ion reducing tests. The polysaccharide of <i>C. rufoaureus</i> has greatly superior antioxidant activity and it could serve as potential functional food or medicine.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":null,"pages":null},"PeriodicalIF":4.6,"publicationDate":"2022-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354634/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40609162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}