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Screening for cellulases and preliminary optimisation of glucose tolerant β-glucosidase production and characterisation. 纤维素酶的筛选和葡萄糖耐受性β-葡萄糖苷酶的初步优化生产和表征。
IF 4.2 2区 生物学 Q1 Medicine Pub Date : 2023-01-01 DOI: 10.1080/21501203.2022.2155261
Nivisti Singh, Bruce Sithole, Roshini Govinden

The search for a novel microbial producer of cellulases including a glucose tolerant β-glucosidase is a challenge as most are inhibited by their product glucose. This study aims to screen for cellulolytic fungi using qualitative and quantitative screening methods. Primary screening revealed 34 of 46 fungal isolates with β-glucosidase activity. Eleven and 13 of these also displayed endoglucanase and exoglucanase activities, respectively. During secondary screening, this number was reduced to 26 β-glucosidase producers with 13 also having endoglucanase and exoglucanase activities. Isolate C1 displayed enhanced production of β-glucosidases in the presence of 0.05 M glucose (69% higher activity). Optimisation of growth conditions for β-glucosidase production by one variable at a time experiments improved production for (isolates) PS1 (64%), MB5 (84%), and C2 (69%). Isolate PS1 identified as Chaetomella sp. BBA70074 displayed the highest tolerance to glucose, retaining 10% of β-glucosidase activity in the presence of 0.8 M glucose. Tolerance to glucose increased to 14% when produced under optimal conditions. β-Glucosidase had a molecular weight of 170 kDa with a pH and temperature optima of 6 and 70°C, respectively. Future studies will include optimisation of the production of the glucose tolerant enzyme by Chaetomella sp. BBA70074.

寻找一种新的纤维素酶微生物生产者包括葡萄糖耐受性β-葡萄糖苷酶是一个挑战,因为大多数被其产物葡萄糖抑制。本研究旨在利用定性和定量筛选方法筛选纤维素分解真菌。初步筛选发现46株真菌中有34株具有β-葡萄糖苷酶活性。其中11个和13个分别表现出内切葡聚糖酶和外切葡聚糖酶活性。在二次筛选时,这一数量减少到26个β-葡萄糖苷酶生产者,其中13个还具有内切葡聚糖酶和外切葡聚糖酶活性。分离物C1在0.05 M葡萄糖的存在下显示出β-葡萄糖苷酶的增强(活性提高69%)。通过单变量优化β-葡萄糖苷酶的生长条件,提高了(分离株)PS1(64%)、MB5(84%)和C2(69%)的产量。分离物PS1鉴定为Chaetomella sp. BBA70074对葡萄糖的耐受性最高,在0.8 M葡萄糖存在下保持10%的β-葡萄糖苷酶活性。在最佳条件下生产时,对葡萄糖的耐受性提高到14%。β-葡萄糖苷酶分子量为170 kDa,最适pH为6℃,最适温度为70℃。未来的研究将包括优化毛霉sp. BBA70074的葡萄糖耐受性酶的生产。
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引用次数: 3
Identification and pathogenicity of six fungal species causing canker and dieback disease on golden rain tree in Beijing, China. 北京地区金雨树溃疡病和枯死病6种真菌的鉴定和致病性。
IF 4.2 2区 生物学 Q1 Medicine Pub Date : 2023-01-01 DOI: 10.1080/21501203.2022.2096144
Meng Pan, Lu Lin, Chengming Tian, Xinlei Fan

Golden rain trees (Koelreuteria paniculata) are largely cultivated because of their important ornamental, medicinal, and economic value. However, they are affected by canker and dieback disease to a large extent. To determine the fungi associated with canker and dieback disease of golden rain trees, isolations were obtained from diseased branches and twigs during 2019 and 2020 in greenbelts and nurseries in Beijing, China. Isolates were identified as six species (Allocryptovalsa castaneicola, Botryosphaeria dothidea, Cytospora koelreutericola sp. nov., Dothiorella acericola, Eutypella citricola, and Peroneutypa scoparia) based on morphological features and phylogenetic analyses of ITS, act, rpb2, tef1-α, and tub2. The results of pathogenicity tests indicated that all fungi produced discoloration and Botryosphaeria dothidea was highly aggressive to golden rain tree. In conclusion, this study explored the taxonomy, phylogeny, and pathogenicity of different fungal species associated with canker and dieback disease on golden rain tree and provided fundamental knowledge to improve disease management.

金雨树(Koelreuteria paniculata)因其重要的观赏、药用和经济价值而被大量种植。然而,它们在很大程度上受到溃疡病和枯死病的影响。为了确定与金雨树溃疡病和枯死病相关的真菌,我们于2019年和2020年在中国北京的绿化带和苗圃中从病枝和细枝中分离出真菌。根据ITS、act、rpb2、tef1-α和tub2的形态特征和系统发育分析,分离菌株鉴定为6个种(异源隐球菌castaneicola、斑点孢球孢菌dotryosphaeria dothicola、斑点孢胞孢子菌koelreutericola sp. nov、斑点微孢子菌Dothiorella acericola、柠檬酸微孢子菌citricola和scoparia Peroneutypa)。致病性试验结果表明,所有真菌均产生变色现象,对金雨树具有极强的侵染性。总之,本研究探讨了与金雨树溃疡病和枯死病相关的不同真菌种类的分类、系统发育和致病性,为提高病害管理水平提供了基础知识。
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引用次数: 2
Metabolomics analysis of mycelial exudates provides insights into fungal antagonists of Armillaria. 菌丝渗出物的代谢组学分析提供了蜜环菌拮抗真菌的见解。
IF 4.2 2区 生物学 Q1 Medicine Pub Date : 2023-01-01 DOI: 10.1080/21501203.2023.2238753
Jian Zhan, Jing Yuan, Jianwei Liu, Fengming Zhang, Fuqiang Yu, Yanliang Wang

The genus Armillaria has high edible and medical values, with zones of antagonism often occurring when different species are paired in culture on agar media, while the antagonism-induced metabolic alteration remains unclear. Here, the metabolome of mycelial exudates of two Chinese Armillaria biological species, C and G, co-cultured or cultured separately was analysed to discover the candidate biomarkers and the key metabolic pathways involved in Armillaria antagonists. A total of 2,377 metabolites were identified, mainly organic acids and derivatives, lipids and lipid-like molecules, and organoheterocyclic compounds. There were 248 and 142 differentially expressed metabolites between group C-G and C, C-G, and G, respectively, and fourteen common differentially expressed metabolites including malate, uracil, Leu-Gln-Arg, etc. Metabolic pathways like TCA cycle and pyrimidine metabolism were significantly affected by C-G co-culture. Additionally, 156 new metabolites (largely organic acids and derivatives) including 32 potential antifungal compounds, primarily enriched into biosynthesis of secondary metabolites pathways were identified in C-G co-culture mode. We concluded that malate and uracil could be used as the candidate biomarkers, and TCA cycle and pyrimidine metabolism were the key metabolic pathways involved in Armillaria antagonists. The metabolic changes revealed in this study provide insights into the mechanisms underlying fungal antagonists.

蜜环菌属具有很高的食用和药用价值,当不同物种在琼脂培养基上配对培养时,经常出现拮抗区,而拮抗诱导的代谢改变尚不清楚。本研究分析了中国两种蜜环菌种C和G共培养或单独培养的菌丝渗出物的代谢组学,以发现蜜环菌拮抗剂的候选生物标志物和关键代谢途径。共鉴定出2377种代谢物,主要是有机酸及其衍生物、脂质和类脂质分子以及有机杂环化合物。C-G组与C组、C-G组和G组差异表达代谢物分别为248种和142种,共有14种差异表达代谢物,包括苹果酸盐、尿嘧啶、Leu-Gln-Arg等。C-G共培养显著影响了TCA循环和嘧啶代谢等代谢途径。此外,在C-G共培养模式下,鉴定出156种新的代谢物(主要是有机酸及其衍生物),包括32种潜在的抗真菌化合物,主要富集到次生代谢物的生物合成途径中。我们认为苹果酸和尿嘧啶可以作为候选生物标志物,TCA循环和嘧啶代谢是蜜环菌拮抗剂的关键代谢途径。本研究揭示的代谢变化为真菌拮抗剂的机制提供了见解。
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引用次数: 0
A critical role of farnesol in the modulation of Amphotericin B and Aureobasidin A antifungal drug susceptibility. 法尼醇在两性霉素B和Aureobasidin A抗真菌药物敏感性调节中的关键作用。
IF 4.2 2区 生物学 Q1 Medicine Pub Date : 2022-10-28 eCollection Date: 2022-01-01 DOI: 10.1080/21501203.2022.2138599
Venkatramanan Mahendrarajan, Vinay Kumar Bari

Candida albicans and its related species can cause opportunistic infections such as "candidiasis" in immunocompromised individuals with a high morbidity and mortality rate. Several antifungal drugs available in the market are often used to treat infections caused by pathogenic fungi. However, in fungi, the development of resistance against these drugs quickly evolved. Candida is a dimorphic fungus that can switch between yeast to hyphae form, requires an active biosynthesis of membrane constituents. Sphingolipid and ergosterol molecules, are the major fungal plasma membrane components, and their interaction with the antifungal drug can modulate drug susceptibility. A lipophilic compound farnesol acts as a quorum-sensing molecule synthesised by the isoprenoid biosynthesis pathway in the fungal pathogen Candida. Farnesol is secreted in a cell density-dependent manner inhibits hyphae germination and biofilm formation. In this study, we have investigated whether the farnesol molecules affect the drug susceptibility of the antifungal drug Amphotericin B (AmB) which mainly binds with ergosterol, and Aureobasidin A (AbA), a complex sphingolipid biosynthesis inhibitor. Our studies revealed that a non-toxic and low concentration of farnesol can reduce the efficacy of AmB and AbA on yeast cells. This reduction is probably through the alteration in the complex sphingolipid biosynthesis and ATP-binding cassette (ABC) type membrane transport activity. These findings may shed light on a new direction to explore the role of lipid molecules in the antifungal drug resistance mechanisms in pathogenic yeast.

白色念珠菌及其相关菌种可在免疫功能低下的个体中引起机会性感染,如“念珠菌病”,发病率和死亡率高。市场上几种抗真菌药物通常用于治疗由致病性真菌引起的感染。然而,真菌对这些药物的耐药性发展很快。念珠菌是一种二态真菌,可以在酵母和菌丝形态之间转换,需要活性的生物合成膜成分。鞘脂和麦角甾醇分子是真菌质膜的主要成分,它们与抗真菌药物的相互作用可以调节药物的敏感性。一种亲脂化合物法尼醇作为群体感应分子,在真菌病原体念珠菌中通过类异戊二烯生物合成途径合成。法尼醇以细胞密度依赖的方式分泌,抑制菌丝萌发和生物膜的形成。在这项研究中,我们研究了法尼醇分子是否影响抗真菌药物两性霉素B (AmB)的药物敏感性,两性霉素B主要与麦角甾醇结合,而Aureobasidin A (AbA)是一种复杂的鞘脂生物合成抑制剂。我们的研究表明,无毒和低浓度的法尼醇可以降低AmB和AbA对酵母细胞的作用。这种减少可能是由于复杂鞘脂生物合成和atp结合盒(ABC)型膜运输活性的改变。这些发现可能为探索脂质分子在病原菌抗真菌耐药机制中的作用提供了新的方向。
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引用次数: 4
Mycobiota and mycotoxin-producing fungi in southern California: their colonisation and in vitro interactions. 南加州的菌群和产生霉菌毒素的真菌:它们的定植和体外相互作用。
IF 4.2 2区 生物学 Q1 Medicine Pub Date : 2022-07-27 eCollection Date: 2022-01-01 DOI: 10.1080/21501203.2022.2104950
Paris Chakravarty

Fungal contamination in water-damaged houses has become a major concern because of their potential health effects. During our survey in 100 water-damaged houses in southern California, we have reported 42 outdoor and 14 indoor fungal species throughout the year. Six commonly occurring indoor fungal species are Alternaria alternata, Aspergillus niger, Chaetomium globosum, Cladosporium herbarum, Penicillium chrysogenum and Stachybotrys chartarum. In the damp building materials, S. chartarum was found to be associated with A. niger, C. globosum and P. chrysogenum but not with A. alternata and C. herbarum. Stachybotrys chartarum showed strong antagonistic effect against A. alternata and C. herbarum and significantly inhibited in vitro growth of A. alternata and C. herbarum but had no effect on A. niger, C. globosum and P. chrysogenum. Two trichothecenes, produced by Stachybotrys sp., trichodermin and trichodermol, significantly inhibited spore germination and in vitro growth of A. alternata and C. herbarum but had no effect on A. niger, C. globosum, P. chrysogenum and S. chartarum. In the damp building materials (drywall, ceiling tile, and oak wood), S. chartarum significantly inhibited the growth of A. alternata and C. herbarum and had no effect on A. niger, C. globosum and P. chrysogenum in these substrata.

由于真菌对健康的潜在影响,被水破坏的房屋中的真菌污染已成为一个主要问题。在我们对南加州100所被水破坏的房屋进行的调查中,我们报告了全年42种室外真菌和14种室内真菌。六种常见的室内真菌是互交霉、黑曲霉、球毛菌、植物枝孢霉、黄化青霉和沙氏葡萄球菌。在潮湿的建筑材料中,炭疽菌与黑孢霉、球孢霉和黄孢霉存在关联,而与交替孢霉和植物孢霉不存在关联。白葡萄球菌对黑蚜和金丝桃均有较强的拮抗作用,并能显著抑制金丝桃和金丝桃的体外生长,但对黑蚜、全球金丝桃和金丝桃无抑制作用。由Stachybotrys sp.、trichodermin和trichodermol产生的两种毛霉烯均能显著抑制a.a alternata和c.a herbarum的孢子萌发和离体生长,但对a.a niger、c.g lobosum、p.c rysogenum和s.c chartarum没有影响。在潮湿的建筑材料(干墙、天花板砖和橡木)中,沙氏菌对交替孢霉和植物孢霉的生长有显著抑制作用,对黑孢霉、球孢霉和黄孢霉的生长没有影响。
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引用次数: 1
New insights into the genus Gyroporus (Gyroporaceae, Boletales), with establishment of four new sections and description of five new species from China. 对石龙子属(石龙子科,Boletales)的新认识,建立了四个新部分,并描述了来自中国的五个新种。
IF 4.6 2区 生物学 Q1 MYCOLOGY Pub Date : 2022-07-05 eCollection Date: 2022-01-01 DOI: 10.1080/21501203.2022.2094012
Ming Zhang, De-Chun Xie, Chao-Qun Wang, Wang Qiu Deng, Tai-Hui Li

Species of Gyroporus from southern China were studied in this study. Based on morphology and molecular phylogenetic analyses of DNA sequences from the nuclear ribosomal internal transcribed spacer (ITS), the nuclear ribosomal large subunit (nrLSU), and the mitochondrial adenosine triphosphate ATP synthase subunit 6 (atp6), Gyroporus was divided into four main branches in the phylogenetic tree, and four sections were firstly proposed i.e. Gyroporus sect. Castaneus, G. sect. Cyanescens, G. sect. Longicystidiatus and G. sect. Pallidus. Five new species, i.e. G. alboluteus, G. atrocyanescens, G. pseudolongicystidiatus, G. pallidus and G. subcaerulescens, were revealed from China, and their phylogenetic positions were also analysed. Among them, G. alboluteus and G. pallidus were nested into the sect. Pallidus, although morphologically similar to G. castaneus; G. atrocyanescens and G. subcaerulescens, with obvious cyanescent oxidation reactions, were nested into the sect. Cyanescens; and G. pseudolongicystidiatus characterised by its long cystidia and was nested into the sect. Longicystidiatus. The new species were formally described and illustrated in the present study, and a key to the sections and species of Gyroporus in China was provided.

本研究对我国南方的陀螺孢属植物进行了研究。根据核核糖体内部转录间隔区(ITS)、核核糖体大亚基(nrLSU)和线粒体三磷酸腺苷ATP合酶6亚基(atp6)DNA序列的形态学和分子系统发育分析,在系统发育树中将Gyroporus分为四个主要分支,并首次提出四个部分,即Gyroporussect。Castaneus,G.教派。青色,G.教派。长孢子虫和G。Pallidus。本文报道了我国白球藻(G.alboluteus)、萎缩绿球藻(G.atrocyanescens)、假长囊藻(G.pseudo-longycystiatatus)、苍白球藻和近囊藻(G.subcerulescens)5个新种,并对它们的系统发育位置进行了分析。其中白球藻和苍白球藻嵌套于该教派。Pallidus,尽管在形态上与G.castaneus相似;萎缩绿脓杆菌和亚绿脓杆菌具有明显的青色氧化反应,嵌套在该节中。青色;和G.假长孢子虫,其特征是其长孢子虫并嵌套在该节中。长孢子虫属。本研究对这些新种进行了正式的描述和说明,并提供了中国陀螺孢属的剖面和种的关键。
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引用次数: 0
Correction notice. 更正通知。
IF 4.2 2区 生物学 Q1 Medicine Pub Date : 2022-06-22 eCollection Date: 2022-01-01 DOI: 10.1080/21501203.2022.2073697

[This corrects the article DOI: 10.1080/21501203.2020.1816584.].

[这更正了文章DOI: 10.1080/21501203.2020.1816584.]。
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引用次数: 0
Isolation, screening, preliminary optimisation and characterisation of thermostable xylanase production under submerged fermentation by fungi in Durban, South Africa. 南非德班地区真菌深层发酵产热稳定木聚糖酶的分离、筛选、初步优化和特性研究。
IF 4.2 2区 生物学 Q1 Medicine Pub Date : 2022-06-20 eCollection Date: 2022-01-01 DOI: 10.1080/21501203.2022.2079745
Priyashini Dhaver, Brett Pletschke, Bruce Sithole, Roshini Govinden

Fungi are renowned for their ability to produce extracellular enzymes into their surrounding environment. Xylanases are hydrolytic enzymes capable of xylan degradation. The objectives of this study were to isolate, screen for potential xylanolytic fungi from soil and tree bark samples from three locations in South Africa and to determine their growth conditions for maximum xylanase production. Forty-six isolates were obtained based on clearing zone formation on xylan-enriched agar plates using Congo red indicator. Xylanase activity was quantified during submerged fermentation. Isolate MS5, identified as Trichoderma harzianum with the highest enzyme activity (38.17 U/ml) was selected for further studies based on thermophilic properties (70°C) and pH (5.0). The culture conditions; incubation period (5 days), agitation speed (160 rpm) wheat bran (1%) and ammonium sulphate (1.2%) were optimised further. Biochemical characterisation of the crude enzyme revealed two pH and temperature optima (pH 6.0 at 60°C and 70°C, pH 8.0 at 55°C and 75°C). The enzyme retained >70% activity after 4 h at pH 6.0 at 70°C. SDS-PAGE revealed multiple protein bands with a prominent band at 70 kDa. Substrate Native PAGE revealed multiple isoforms between 55 and 130 kDa. This enzyme will be beneficial for applications in the animal feed and biofuels industries.

真菌以其在周围环境中产生胞外酶的能力而闻名。木聚糖酶是能够降解木聚糖的水解酶。本研究的目的是从南非三个地点的土壤和树皮样品中分离、筛选潜在的木聚糖分解真菌,并确定其最大木聚糖酶产量的生长条件。利用刚果红指示剂对富木聚糖琼脂板上形成的清除带进行筛选,得到46株分离菌。在深层发酵过程中定量测定木聚糖酶活性。分离物MS5经鉴定为哈兹木霉,酶活性最高(38.17 U/ml),根据其嗜热特性(70°C)和pH(5.0)选择进一步研究。培养条件;进一步优化培养时间(5 d)、搅拌速度(160 rpm)、麦麸(1%)和硫酸铵(1.2%)。粗酶的生化特性显示两个pH和温度最优(60℃和70℃时pH 6.0, 55℃和75℃时pH 8.0)。在pH 6.0、70℃条件下,酶活性维持4 h,活性保持>70%。SDS-PAGE显示在70 kDa处有多条突出的蛋白带。底物Native PAGE在55 ~ 130 kDa范围内显示多个同工异构体。这种酶将有利于动物饲料和生物燃料工业的应用。
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引用次数: 9
What is the correct scientific name for "Fuling" medicinal mushroom? “涪陵”药菇的正确学名是什么?
IF 4.2 2区 生物学 Q1 Medicine Pub Date : 2022-06-20 eCollection Date: 2022-01-01 DOI: 10.1080/21501203.2022.2089755
Viktor Papp, Yu-Cheng Dai

In recent years, the scientific names of many cultivated and well-known medicinal fungal species have been changed. However, the results of taxonomic and nomenclature works on these economically important fungi are often overlooked or ignored in applied researches. The incorrect use of scientific names may cause uncertainty in research and in the global medicinal mushroom market. In this paper, we briefly review the current taxonomy and nomenclature of "Fuling" medicinal mushroom and make a proposal for biochemists, pharmacists and businessmen on the correct use of scientific names related to this species. Based on the recent taxonomic results and nomenclatural proposals, the use of the names Wolfiporia extensa, W. cocos and especially Poria cocos for the "Fuling" mushroom are incorrect and misleading; therefore, the acceptance of the names Pachyma hoelen or Wolfiporia hoelen is recommended.

近年来,许多栽培和知名的药用真菌物种的学名发生了变化。然而,对这些具有重要经济意义的真菌的分类和命名工作的结果在应用研究中往往被忽视或忽视。学名的不正确使用可能会导致研究和全球药用蘑菇市场的不确定性。本文简要回顾了“涪陵”药用菌的分类和命名现状,并对生化学家、药剂师和商人正确使用“涪陵”药用菌的学名提出了建议。根据最近的分类结果和命名建议,将“涪陵”蘑菇命名为Wolfiporia extensa, W. cocos,特别是Poria cocos是不正确的,具有误导性;因此,建议接受Pachyma hoelen或Wolfiporia hoelen的名称。
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引用次数: 1
Preparation, structural characterisation, and antioxidant activities of polysaccharides from eight boletes (Boletales) in tropical China. 中国热带地区八种牛肝菌多糖的制备、结构特征和抗氧化活性。
IF 4.6 2区 生物学 Q1 MYCOLOGY Pub Date : 2022-05-06 eCollection Date: 2022-01-01 DOI: 10.1080/21501203.2022.2069172
Run Tian, Hui Chai, Jun-Qiang Qiu, Zhi-Qun Liang, Hui-Jing Xie, Yong Wang, Nian-Kai Zeng

Polysaccharides in boletes (Boletales) are economically significant to both function food and medicinal industries. The polysaccharides were extracted from the fruit bodies of eight boletes, namely, Aureoboletus longicollis, Butyriboletus hainanensis, Crocinoboletus rufoaureus, Hemioporus japonicus, Neoboletus infuscatus, Neoboletus obscureumbrinus, Tylopilus otsuensis, Xanthoconium fusciceps, which were collected from tropical China; their physicochemical properties and antioxidant activities were characterised and evaluated, respectively. The results revealed that the polysaccharides among the eight boletes were mainly composed of glucose, mannose, and galactose, with a broad molecular weight range, and contained a pyranose ring revealed by FT-IR and NMR spectral analyses. Many factors such as different species of boletes, geographic conditions, molecular weight, configuration, and monosaccharide content may affect the antioxidant power of polysaccharides, simultaneously, instead of one single factor. The antioxidant activities of the polysaccharides were measured according to in vitro assays of DPPH scavenging, superoxide anion scavenging, and ferrous ion reducing tests. The polysaccharide of C. rufoaureus has greatly superior antioxidant activity and it could serve as potential functional food or medicine.

牛肝菌(牛肝菌属)中的多糖对功能食品和医药行业都具有重要的经济意义。本研究从中国热带地区采集的八种牛肝菌(Aureoboletus longicollis、Butyriboletus hainanensis、Crocinoboletus rufoaureus、Hemioporus japonicus、Neoboletus infuscatus、Neoboletus obscureumbrinus、Tylopilus otsuensis、Xanthoconium fusciceps)的子实体中提取了多糖;分别对其理化性质和抗氧化活性进行了表征和评价。结果表明,八种牛肝菌的多糖主要由葡萄糖、甘露糖和半乳糖组成,分子量范围较宽,且含有吡喃糖环。不同种类的牛肝菌、地理条件、分子量、构型和单糖含量等多种因素可能同时影响多糖的抗氧化能力,而不是单一因素。多糖的抗氧化活性是通过 DPPH 清除、超氧阴离子清除和亚铁离子还原等体外试验来测定的。红豆杉多糖具有极高的抗氧化活性,可作为潜在的功能性食品或药物。
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引用次数: 0
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Mycology
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