Pub Date : 2024-08-03DOI: 10.1177/1934578x241265216
Ji Hye Kim, Sooyeon Kang, Gyu-Ri Lee, Seong-Gyu Ko
BackgroundTumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is well known to selectively induce apoptotic cell death in cancer cells, not in normal cells, with death receptors (DRs)—DR4 and DR5. In consequence of this specialty, this cytokine and its receptors are considered for candidates of target therapy in clinic. SH003, a new traditional medicine-based polyherbal preparation, consists of Astragalus membranaceus (Am), Angelica gigas (Ag) and Trichosanthes kirilowii Maximowicz (Tk). In this study, we investigated whether SH003 can induce apoptosis through DRs in non-small-cell lung cancer (NSCLC) cells.MethodsCell proliferation and cytotoxicity were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), clonogenic assay, and, trypan blue exclusion staining, protein expression by western blot analysis, and apoptosis by fluorescence-activated cell sorting analysis.ResultsWe found that SH003-induced apoptosis in NSCLC cells through several mechanisms. First of all, MTT and colony formation assay confirmed the growth-inhibitory effect of SH003 in H460 cells. Second, SH003 upregulated the expression of DR4 and DR5. Third, it activated caspase-8, caspase-7, and caspase-3 cascades, which are essential for DR-mediated extrinsic apoptosis. The effect of SH003-induced apoptosis was significantly abolished by inhibition of caspases enzymes. And also, SH003 cleaved caspse-9. Fourth, SH003 reduced AKT kinase phosphorylation, and overexpression of AKT abrogated the caspase-dependent apoptosis by SH003. Fifth, SH003 inactivated ERK, but, constitutive ERK expression did not completely reduce SH003-mediated growth inhibition and apoptosis.ConclusionsSH003 potentiates caspase-dependent apoptosis of NSCLC through the upregulation of DRs, activation of caspase cascades and downregulation of AKT cell survival pathways.
{"title":"SH003 Induces DR5-Mediated Caspase-Dependent Apoptosis of NSCLC Through Inhibition of AKT Survival Pathway","authors":"Ji Hye Kim, Sooyeon Kang, Gyu-Ri Lee, Seong-Gyu Ko","doi":"10.1177/1934578x241265216","DOIUrl":"https://doi.org/10.1177/1934578x241265216","url":null,"abstract":"BackgroundTumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is well known to selectively induce apoptotic cell death in cancer cells, not in normal cells, with death receptors (DRs)—DR4 and DR5. In consequence of this specialty, this cytokine and its receptors are considered for candidates of target therapy in clinic. SH003, a new traditional medicine-based polyherbal preparation, consists of Astragalus membranaceus (Am), Angelica gigas (Ag) and Trichosanthes kirilowii Maximowicz (Tk). In this study, we investigated whether SH003 can induce apoptosis through DRs in non-small-cell lung cancer (NSCLC) cells.MethodsCell proliferation and cytotoxicity were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), clonogenic assay, and, trypan blue exclusion staining, protein expression by western blot analysis, and apoptosis by fluorescence-activated cell sorting analysis.ResultsWe found that SH003-induced apoptosis in NSCLC cells through several mechanisms. First of all, MTT and colony formation assay confirmed the growth-inhibitory effect of SH003 in H460 cells. Second, SH003 upregulated the expression of DR4 and DR5. Third, it activated caspase-8, caspase-7, and caspase-3 cascades, which are essential for DR-mediated extrinsic apoptosis. The effect of SH003-induced apoptosis was significantly abolished by inhibition of caspases enzymes. And also, SH003 cleaved caspse-9. Fourth, SH003 reduced AKT kinase phosphorylation, and overexpression of AKT abrogated the caspase-dependent apoptosis by SH003. Fifth, SH003 inactivated ERK, but, constitutive ERK expression did not completely reduce SH003-mediated growth inhibition and apoptosis.ConclusionsSH003 potentiates caspase-dependent apoptosis of NSCLC through the upregulation of DRs, activation of caspase cascades and downregulation of AKT cell survival pathways.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"2 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141932952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Selected compounds from Centella asiatica with proven neuroprotective potential and analyzed strong binding affinity with molecular docking can be considered as potential inhibitors for alpha-1-antichymotrypsin in Alzheimer's disease.
{"title":"Asiatic Acid, Quercetin, and Kaempferol From Centella asiatica as Potential Inhibitors of Alpha-1-Antichymotrypsin in Alzheimer's Disease","authors":"Jhinuk Chatterjee, Apoorva Atmuri, Eshaana Janardhan Raichur, Gouri Anil","doi":"10.1177/1934578x241264637","DOIUrl":"https://doi.org/10.1177/1934578x241264637","url":null,"abstract":"Selected compounds from Centella asiatica with proven neuroprotective potential and analyzed strong binding affinity with molecular docking can be considered as potential inhibitors for alpha-1-antichymotrypsin in Alzheimer's disease.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"57 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141932955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-02DOI: 10.1177/1934578x241264468
Ali Aberoumand, Najmeh Ahmadi Nooradinvand, Rania Kouti, Fatemeh Hyderi
Objective/background: Different processing methods can maintain quality and ensure access to fish throughout year. In Iran, fish can be major source of affordable dietary protein for human nutrition. Fish ( Carangoides armatus) locally known as Gish, is most preferred and highest value commercial food fish species obtained from southern Iran. Methods: This article examined impact of different processes on the physicochemical properties of fresh fish. Results: Highest and lowest values for crude protein reported in fish processed using high temperature short time (HTST) in autoclave (17.51%) and salting (8.47%), respectively ( P < .05), compared to (16.69%) for fresh fish (control). Fish processed with 20% salt concluded significantly higher crude fat content (69.45%) ( P < .05) followed by samples prepared using 10% salt (68.88%), while lowest value (9.12%) found for fish marinated T1. The results suggested that fish processed with HTST found higher nutritional quality principally due to relatively high content of most needed nutrient—protein. Adopting and encouraging use of autoclave could also be a way of saving energy. Fish components that affected by processes are proximate composition and energy. Conclusions: Changes in the physicochemical and nutritional factors of processed fish affect its final quality. Gish fish ( C armatus) caught from the southern regions of Iran had a high percentage of protein and fat, which has a good potential for the growth of children and to prevent stunting.
{"title":"Effect of Different Processes to Nutrient Profile of Dead Fresh Fish Carangoides armatus as Potential Protein-Rich Seafood","authors":"Ali Aberoumand, Najmeh Ahmadi Nooradinvand, Rania Kouti, Fatemeh Hyderi","doi":"10.1177/1934578x241264468","DOIUrl":"https://doi.org/10.1177/1934578x241264468","url":null,"abstract":"Objective/background: Different processing methods can maintain quality and ensure access to fish throughout year. In Iran, fish can be major source of affordable dietary protein for human nutrition. Fish ( Carangoides armatus) locally known as Gish, is most preferred and highest value commercial food fish species obtained from southern Iran. Methods: This article examined impact of different processes on the physicochemical properties of fresh fish. Results: Highest and lowest values for crude protein reported in fish processed using high temperature short time (HTST) in autoclave (17.51%) and salting (8.47%), respectively ( P < .05), compared to (16.69%) for fresh fish (control). Fish processed with 20% salt concluded significantly higher crude fat content (69.45%) ( P < .05) followed by samples prepared using 10% salt (68.88%), while lowest value (9.12%) found for fish marinated T1. The results suggested that fish processed with HTST found higher nutritional quality principally due to relatively high content of most needed nutrient—protein. Adopting and encouraging use of autoclave could also be a way of saving energy. Fish components that affected by processes are proximate composition and energy. Conclusions: Changes in the physicochemical and nutritional factors of processed fish affect its final quality. Gish fish ( C armatus) caught from the southern regions of Iran had a high percentage of protein and fat, which has a good potential for the growth of children and to prevent stunting.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"75 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141881987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-02DOI: 10.1177/1934578x241266706
Sungmin Hwang, Ji Soo Kim, Go Kyoung Na, Hyeon Jeong Lee, Bohyun Yun, Ji-Won Park, Seahee Han, Min-Ju Park, WonWoo Lee, Kyung-Min Choi, Jung Up Park
ObjectiveThe rich botanical biodiversity resulting from diverse climates and geographical distinctiveness offers a plethora of biological resources that can be pivotal in developing innovative biomaterials. This investigation sought to evaluate the functional properties of indigenous Korean plant species.MethodsForty-six indigenous plants during a year-long expedition across inhabited and uninhabited Korean islands were collected. The plant specimens were divided into 5 parts (flower, fruit, stem, leaf, and whole body) and subjected to extraction using water, 30% ethanol, and 70% ethanol, followed by characterizations of anti-inflammatory, immune response, and anti-bacterial activity.ResultsThirty-eight percent of the extracts (59 extracts from 31 species) exhibited statistically significant anti-inflammatory effects. Concurrently, 22% of the extracts (35 extracts from 24 species) demonstrated notable immune boosting effects. Additionally, 17 extracts exhibited significant inhibitory effects against the growth of pathogenic bacteria.ConclusionThis study highlights the potential utility of extracts of plants from Korean islands as natural agents for next-generation pharmaceutical and medical applications by emphasizing their effectiveness in combating inflammation, enhancing immune responses, and conferring anti-bacterial benefits.
{"title":"Characterization of the Anti-Inflammatory, Immune Response, and Anti-Bacterial Properties of the Polar Extracts of Indigenous Korean Plants","authors":"Sungmin Hwang, Ji Soo Kim, Go Kyoung Na, Hyeon Jeong Lee, Bohyun Yun, Ji-Won Park, Seahee Han, Min-Ju Park, WonWoo Lee, Kyung-Min Choi, Jung Up Park","doi":"10.1177/1934578x241266706","DOIUrl":"https://doi.org/10.1177/1934578x241266706","url":null,"abstract":"ObjectiveThe rich botanical biodiversity resulting from diverse climates and geographical distinctiveness offers a plethora of biological resources that can be pivotal in developing innovative biomaterials. This investigation sought to evaluate the functional properties of indigenous Korean plant species.MethodsForty-six indigenous plants during a year-long expedition across inhabited and uninhabited Korean islands were collected. The plant specimens were divided into 5 parts (flower, fruit, stem, leaf, and whole body) and subjected to extraction using water, 30% ethanol, and 70% ethanol, followed by characterizations of anti-inflammatory, immune response, and anti-bacterial activity.ResultsThirty-eight percent of the extracts (59 extracts from 31 species) exhibited statistically significant anti-inflammatory effects. Concurrently, 22% of the extracts (35 extracts from 24 species) demonstrated notable immune boosting effects. Additionally, 17 extracts exhibited significant inhibitory effects against the growth of pathogenic bacteria.ConclusionThis study highlights the potential utility of extracts of plants from Korean islands as natural agents for next-generation pharmaceutical and medical applications by emphasizing their effectiveness in combating inflammation, enhancing immune responses, and conferring anti-bacterial benefits.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"53 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141881986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: The Zuo Jin pill (ZJP), a classic formula for treating gastrointestinal diseases, has demonstrated good efficacy in the treatment of ulcerative colitis (UC). In this study, we aimed to elucidate the mechanism of action of ZJP in UC through network pharmacology and experimental validation. Methods: Bioactive compounds and targets of ZJP, along with UC-related targets, were retrieved from public databases. The intersecting targets of ZJP and UC were visualized using a Venn diagram. Protein–protein interactions and complex target networks were established using Cytoscape software. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were used to enrich the biological functions and pathways of potential targets and to establish a biological process-target-pathway network. Relevant pathways were screened based on literature review and node size, and the interactions between ZJP and UC as well as hub target proteins were verified through animal experiments. Results: The findings suggested that STAT3, AKT1, EGFR, PIK3R1, and various cancer pathways exhibited potential as pivotal targets and biological processes contributing to the therapeutic efficacy of ZJP in UC. These targets were intricately associated with molecular functions, drug response, protein autophosphorylation, and protein kinase binding. Subsequent experimental intervention using ZJP on dextran sulfate sodium-induced UC mice revealed that its mode of action may involve inhibiting the PI3K/AKT/mTOR signaling pathway, mitigating intestinal mucosal inflammation, and modulating apoptosis in intestinal epithelial cells. Conclusions: The findings suggested that STAT3, AKT1, EGFR, PIK3R1, and various cancer pathways exhibited potential as pivotal targets and biological processes contributing to the therapeutic efficacy of ZJP in UC. These targets were associated with drug response, protein autophosphorylation, and protein kinase binding. Further experiments revealed that its mode of action may involve inhibiting the PI3K/AKT/mTOR signaling pathway, mitigating intestinal mucosal inflammation, and modulating apoptosis in intestinal epithelial cells.
{"title":"Investigating Molecular Mechanisms Underlying the Therapeutic Effects of Zuo Jin Pill in Ulcerative Colitis: A Combined Approach of Network Pharmacology and Experimental Validation","authors":"Xiao Xiao, Lihong Wu, Chuanyu Zhan, Zenghua Xiao, Wei Ge, Wu Liao, Leichang Zhang","doi":"10.1177/1934578x241264999","DOIUrl":"https://doi.org/10.1177/1934578x241264999","url":null,"abstract":"Objective: The Zuo Jin pill (ZJP), a classic formula for treating gastrointestinal diseases, has demonstrated good efficacy in the treatment of ulcerative colitis (UC). In this study, we aimed to elucidate the mechanism of action of ZJP in UC through network pharmacology and experimental validation. Methods: Bioactive compounds and targets of ZJP, along with UC-related targets, were retrieved from public databases. The intersecting targets of ZJP and UC were visualized using a Venn diagram. Protein–protein interactions and complex target networks were established using Cytoscape software. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were used to enrich the biological functions and pathways of potential targets and to establish a biological process-target-pathway network. Relevant pathways were screened based on literature review and node size, and the interactions between ZJP and UC as well as hub target proteins were verified through animal experiments. Results: The findings suggested that STAT3, AKT1, EGFR, PIK3R1, and various cancer pathways exhibited potential as pivotal targets and biological processes contributing to the therapeutic efficacy of ZJP in UC. These targets were intricately associated with molecular functions, drug response, protein autophosphorylation, and protein kinase binding. Subsequent experimental intervention using ZJP on dextran sulfate sodium-induced UC mice revealed that its mode of action may involve inhibiting the PI3K/AKT/mTOR signaling pathway, mitigating intestinal mucosal inflammation, and modulating apoptosis in intestinal epithelial cells. Conclusions: The findings suggested that STAT3, AKT1, EGFR, PIK3R1, and various cancer pathways exhibited potential as pivotal targets and biological processes contributing to the therapeutic efficacy of ZJP in UC. These targets were associated with drug response, protein autophosphorylation, and protein kinase binding. Further experiments revealed that its mode of action may involve inhibiting the PI3K/AKT/mTOR signaling pathway, mitigating intestinal mucosal inflammation, and modulating apoptosis in intestinal epithelial cells.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"41 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141778362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-25DOI: 10.1177/1934578x241250204
Van Cuong Pham, Thi Hue Nguyen, Thuy Linh Nguyen, Mai Anh Nguyen, Thi Quyen Vu, Thi Thu Huyen Vu, Thi Hong Minh Le, Thi Mai Huong Doan
ObjectivesThis study focused on the isolation, structural elucidation, and antimicrobial activities of compounds from the culture broth of the strain Penicillium sp. OM01 (isolated from the marine mollusk sample Spondylus squamosus (Schreibers, 1793)).MethodsVarious chromatographic methods were used to isolate compounds from the ethyl acetate and MeOH extracts of the strain Penicillium sp. OM01. Their structures were elucidated using HR-ESI-MS and NMR data. The compounds were evaluated for antimicrobial effects using the dilution turbidimetric broth method.ResultsOne new alkaloid, penicritin (1), together with seven known compounds, including phenol A acid (2), topsentisteron D3 (3), ergostanol (4), (22 E,24 R)-24-methylcholesta-5,22-diene-3 β, 7 β-diol (5), 5 α,6 α-epoxy-(22 E,24 R)-ergosta-8,22-diene-3 β,7 α-diol (6), staphylopeptide A (7), and 4-hydroxybenzoic acid (8) were isolated from the culture broth of the marine-derived fungus Penicillium sp. OM01. Compounds 2-4 and 6 exhibited antibacterial activity against Gram-(+) bacteria, Enterococcus faecalis, Staphylococcus aureus, Bacillus cereus, and yeast Candida albicans with MIC values ranging from 32 to 256 µg/mL.ConclusionsThis is the first report of the strain Penicillium sp. OM01 from the marine mollusk sample S. squamosus (Schreibers, 1793). One new and seven known compounds were isolated from the strain Penicillium sp. OM01 (isolated from the marine mollusk sample S. squamosus (Schreibers, 1793). Compound 4 is a potential antibacterial agent.
{"title":"Antimicrobial Activities of a New Alkaloid from Marine-Derived Fungus, Penicillium sp. OM01","authors":"Van Cuong Pham, Thi Hue Nguyen, Thuy Linh Nguyen, Mai Anh Nguyen, Thi Quyen Vu, Thi Thu Huyen Vu, Thi Hong Minh Le, Thi Mai Huong Doan","doi":"10.1177/1934578x241250204","DOIUrl":"https://doi.org/10.1177/1934578x241250204","url":null,"abstract":"ObjectivesThis study focused on the isolation, structural elucidation, and antimicrobial activities of compounds from the culture broth of the strain Penicillium sp. OM01 (isolated from the marine mollusk sample Spondylus squamosus (Schreibers, 1793)).MethodsVarious chromatographic methods were used to isolate compounds from the ethyl acetate and MeOH extracts of the strain Penicillium sp. OM01. Their structures were elucidated using HR-ESI-MS and NMR data. The compounds were evaluated for antimicrobial effects using the dilution turbidimetric broth method.ResultsOne new alkaloid, penicritin (1), together with seven known compounds, including phenol A acid (2), topsentisteron D<jats:sub>3</jats:sub> (3), ergostanol (4), (22 E,24 R)-24-methylcholesta-5,22-diene-3 β, 7 β-diol (5), 5 α,6 α-epoxy-(22 E,24 R)-ergosta-8,22-diene-3 β,7 α-diol (6), staphylopeptide A (7), and 4-hydroxybenzoic acid (8) were isolated from the culture broth of the marine-derived fungus Penicillium sp. OM01. Compounds 2-4 and 6 exhibited antibacterial activity against Gram-(+) bacteria, Enterococcus faecalis, Staphylococcus aureus, Bacillus cereus, and yeast Candida albicans with MIC values ranging from 32 to 256 µg/mL.ConclusionsThis is the first report of the strain Penicillium sp. OM01 from the marine mollusk sample S. squamosus (Schreibers, 1793). One new and seven known compounds were isolated from the strain Penicillium sp. OM01 (isolated from the marine mollusk sample S. squamosus (Schreibers, 1793). Compound 4 is a potential antibacterial agent.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"8 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141778364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-25DOI: 10.1177/1934578x241262909
Shaojun Wang, Fanglin Lai, Ting Xiang, Yan Xu
ObjectiveTo systematically explore the targets and signaling pathways of sinomenine (SIN) in the treatment of osteoarthritis (OA) using integrated network pharmacology, molecular docking, and experimental validation.MethodsThe TCMSP, SwissADME, and Pharmmapper databases were used to predict SIN targets, while the databases of GeneCards, DisGeNET, OMIM, and DrugBank were selected to acquire OA targets. Subsequently, the intersection targets of SIN and OA disease were collected using the Veeny platform. Then, the protein-protein interaction (PPI) network map of “SIN-targets-OA” was established using String database and Cytoscape software. Additionally, the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed through the Database for Annotation, Visualization and Integrated Discovery (DAVID). Additionally, the potential proteins of SIN against OA were validated via molecular docking technique. Finally, the experimental validation was performed in SW1353 cells induced by interleukin (IL)-1β.ResultsA total of 315 potential targets of SIN and 4300 OA-associated targets were collected from public databases, and 42 intersecting potential targets of SIN and OA disease acquired. Then, the PPI network diagram of “SIN-targets-OA” was acquired that comprised a total of 43 nodes and 82 edges. Moreover, 173 GO and 21 KEGG pathway entries were screened with a P-value <.05. Among them, peroxisome proliferator-activated receptor (PPAR) and IL-17 are the core signaling pathways. Molecular docking technique indicated strong binding energies of SIN with PPAR (−6.1 kcal/mol) and IL-17 (−6.3 kcal/mol). Lastly, SIN at the concentration of 50 μmol/L has a significant effect on IL-1β-induced SW1353 cells by the inhibition of PPAR-γ and IL-17A proteins without cytotoxicity.ConclusionThis work revealed the underlying targets and signaling pathways of SIN against OA using integrated network pharmacology molecular docking, and experimental validation. These findings provide scientific evidence for the clinical application of SIN for OA treatment.
方法 利用 TCMSP、SwissADME 和 Pharmmapper 数据库预测 SIN 靶点，同时选择 GeneCards、DisGeNET、OMIM 和 DrugBank 数据库获取 OA 靶点。随后，利用 Veeny 平台收集了 SIN 和 OA 疾病的交叉靶点。然后，利用 String 数据库和 Cytoscape 软件建立了 "SIN-靶点-OA "的蛋白-蛋白相互作用（PPI）网络图。此外，还通过注释、可视化和综合发现数据库（DAVID）进行了基因本体（GO）和京都基因组百科全书（KEGG）通路富集分析。此外，还通过分子对接技术验证了 SIN 针对 OA 的潜在蛋白。最后，在白细胞介素（IL）-1β诱导的SW1353细胞中进行了实验验证。结果从公共数据库中共收集到315个SIN潜在靶点和4300个OA相关靶点，并获得了42个SIN和OA疾病的交叉潜在靶点。然后，得到了 "SIN-靶点-OA "PPI 网络图，该网络图由 43 个节点和 82 条边组成。此外，还筛选出 173 个 P 值为 0.05 的 GO 和 21 个 KEGG 通路条目。其中，过氧化物酶体增殖激活受体（PPAR）和 IL-17 是核心信号通路。分子对接技术表明，SIN 与 PPAR（-6.1 kcal/mol）和 IL-17 （-6.3 kcal/mol）的结合能很强。最后，浓度为 50 μmol/L 的 SIN 通过抑制 PPAR-γ 和 IL-17A 蛋白，对 IL-1β 诱导的 SW1353 细胞有显著效果，且无细胞毒性。这些发现为 SIN 治疗 OA 的临床应用提供了科学依据。
{"title":"Integrated Network Pharmacology, Molecular Docking, and Experimental Validation to Explore Potential Mechanisms of Sinomenine in the Treatment of Osteoarthritis","authors":"Shaojun Wang, Fanglin Lai, Ting Xiang, Yan Xu","doi":"10.1177/1934578x241262909","DOIUrl":"https://doi.org/10.1177/1934578x241262909","url":null,"abstract":"ObjectiveTo systematically explore the targets and signaling pathways of sinomenine (SIN) in the treatment of osteoarthritis (OA) using integrated network pharmacology, molecular docking, and experimental validation.MethodsThe TCMSP, SwissADME, and Pharmmapper databases were used to predict SIN targets, while the databases of GeneCards, DisGeNET, OMIM, and DrugBank were selected to acquire OA targets. Subsequently, the intersection targets of SIN and OA disease were collected using the Veeny platform. Then, the protein-protein interaction (PPI) network map of “SIN-targets-OA” was established using String database and Cytoscape software. Additionally, the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed through the Database for Annotation, Visualization and Integrated Discovery (DAVID). Additionally, the potential proteins of SIN against OA were validated via molecular docking technique. Finally, the experimental validation was performed in SW1353 cells induced by interleukin (IL)-1β.ResultsA total of 315 potential targets of SIN and 4300 OA-associated targets were collected from public databases, and 42 intersecting potential targets of SIN and OA disease acquired. Then, the PPI network diagram of “SIN-targets-OA” was acquired that comprised a total of 43 nodes and 82 edges. Moreover, 173 GO and 21 KEGG pathway entries were screened with a P-value <.05. Among them, peroxisome proliferator-activated receptor (PPAR) and IL-17 are the core signaling pathways. Molecular docking technique indicated strong binding energies of SIN with PPAR (−6.1 kcal/mol) and IL-17 (−6.3 kcal/mol). Lastly, SIN at the concentration of 50 μmol/L has a significant effect on IL-1β-induced SW1353 cells by the inhibition of PPAR-γ and IL-17A proteins without cytotoxicity.ConclusionThis work revealed the underlying targets and signaling pathways of SIN against OA using integrated network pharmacology molecular docking, and experimental validation. These findings provide scientific evidence for the clinical application of SIN for OA treatment.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"11 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141778363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Walnut green husk (WGH) is the immature exocarp of Juglans regia L., a famous traditional herbal medicine that has long been used in China, Korea, Iran, and other places for the treatment of psoriasis, baldness, type II diabetes mellitus, intestinal worms, promotion of wound healing, and other diseases. With the advancement of modern scientific research, many studies have identified that WGH contains quinones, diarylheptane, triterpenoids, and flavonoids, and has excellent antitumor, anti-inflammatory, antibacterial, and antioxidant properties. These findings support the ethnopharmacological application of WGH and highlight the need for further investigation of the plant's potential uses in pharmaceutical fields. In this article, we made a comprehensive review of the traditional applications, botany, chemical components, pharmacological effects, and factors affecting the therapeutic properties of WGH based on a scientific literature review with a view to provide the latest information and research direction for further study and potential use of WGH. All data are gathered from scientific databases including Google Scholar, Pubmed, Sci-Finder, Web of Science, ScienceDirect, and China National Knowledge Infrastructure.
核桃青皮（WGH）是胡桃（Juglans regia L.）的未成熟外果皮，是一种著名的传统中药材，在中国、韩国、伊朗等地长期用于治疗牛皮癣、斑秃、Ⅱ型糖尿病、肠道蠕虫、促进伤口愈合等疾病。随着现代科学研究的发展，许多研究发现，WGH 含有醌类化合物、二芳基庚烷、三萜类化合物和黄酮类化合物，具有良好的抗肿瘤、抗炎、抗菌和抗氧化特性。这些发现支持了WGH的民族药理学应用，并强调了进一步研究该植物在医药领域潜在用途的必要性。本文在科学文献综述的基础上，对WGH的传统应用、植物学、化学成分、药理作用和影响治疗特性的因素进行了全面综述，以期为WGH的进一步研究和潜在用途提供最新信息和研究方向。所有数据均来自科学数据库，包括 Google Scholar、Pubmed、Sci-Finder、Web of Science、ScienceDirect 和中国国家知识基础设施。
{"title":"Traditional Applications, Ethnopharmacology, and Phytochemistry of Walnut Green Husk (Juglans regia L.): A Review","authors":"Bingge Li, Chengqian Cui, Caifang Zhang, Junmei Liu, Fusheng Hao, Lu Han, Changcai Bai, Shijie Wei","doi":"10.1177/1934578x241262156","DOIUrl":"https://doi.org/10.1177/1934578x241262156","url":null,"abstract":"Walnut green husk (WGH) is the immature exocarp of Juglans regia L., a famous traditional herbal medicine that has long been used in China, Korea, Iran, and other places for the treatment of psoriasis, baldness, type II diabetes mellitus, intestinal worms, promotion of wound healing, and other diseases. With the advancement of modern scientific research, many studies have identified that WGH contains quinones, diarylheptane, triterpenoids, and flavonoids, and has excellent antitumor, anti-inflammatory, antibacterial, and antioxidant properties. These findings support the ethnopharmacological application of WGH and highlight the need for further investigation of the plant's potential uses in pharmaceutical fields. In this article, we made a comprehensive review of the traditional applications, botany, chemical components, pharmacological effects, and factors affecting the therapeutic properties of WGH based on a scientific literature review with a view to provide the latest information and research direction for further study and potential use of WGH. All data are gathered from scientific databases including Google Scholar, Pubmed, Sci-Finder, Web of Science, ScienceDirect, and China National Knowledge Infrastructure.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"24 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141778603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-25DOI: 10.1177/1934578x241262895
Qing Liu, Yingfeng Ma, Hanxiao Fan, Jialu Li, Jinbao Tang, Tianjie Wang, Ying Fan, Jianming Su, Hongyu Lei
Objectives: Chikusetsu saponin IVa (CHS-IVa) is a saponin compound widely found in herbs such as Panax japonicus C.A.Mey, which has various functions. The purpose of this study was to investigate the protective effect of CHS-IVa on oxidative stress and inflammation in porcine intestinal epithelial cells (IPEC-J2) under the action of hydrogen peroxide (H2O2). Methods: Cell viability was detected by MTT method. The cell scratch experiment was used to test the repair ability of CHS-IVa on cells. The levels of lactate dehydrogenase (LDH), catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), thioredoxin reductase (TrxR), and malondialdehyde (MDA) were tested using the relevant kits. The expression levels of TNF-α, NF-κB, IL-6, IL-1α, and P65 were detected by qPCR. Results: MTT results showed that CHS-IVa at 50 to 100 μg/mL could enhance the activity of IPEC-J2 cells within 24 hours. CHS-IVa at 80 and 100 μg/mL could promote damage repair and effectively inhibit the release of LDH production in IPEC-J2 cells after exposure to H2O2. Additionally, it had a restorative effect on the loss of TrxR, SOD, GSH, and CAT. At the same time, the treatment of CHS-IVa significantly decreased the mRNA expression of NF-κB, IL-6, and P65 after H2O2 treatment. Conclusion: These findings indicate that a specific concentration of CHS-IVa has a protective effect on IPEC during oxidative stress, thereby enhancing their ability to repair damage.
{"title":"Protective Effect of Chikusetsu saponin IVa on Hydrogen Peroxide Induced Injury of IPEC-J2 Cells","authors":"Qing Liu, Yingfeng Ma, Hanxiao Fan, Jialu Li, Jinbao Tang, Tianjie Wang, Ying Fan, Jianming Su, Hongyu Lei","doi":"10.1177/1934578x241262895","DOIUrl":"https://doi.org/10.1177/1934578x241262895","url":null,"abstract":"Objectives: Chikusetsu saponin IVa (CHS-IVa) is a saponin compound widely found in herbs such as Panax japonicus C.A.Mey, which has various functions. The purpose of this study was to investigate the protective effect of CHS-IVa on oxidative stress and inflammation in porcine intestinal epithelial cells (IPEC-J2) under the action of hydrogen peroxide (H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub>). Methods: Cell viability was detected by MTT method. The cell scratch experiment was used to test the repair ability of CHS-IVa on cells. The levels of lactate dehydrogenase (LDH), catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), thioredoxin reductase (TrxR), and malondialdehyde (MDA) were tested using the relevant kits. The expression levels of TNF-α, NF-κB, IL-6, IL-1α, and P65 were detected by qPCR. Results: MTT results showed that CHS-IVa at 50 to 100 μg/mL could enhance the activity of IPEC-J2 cells within 24 hours. CHS-IVa at 80 and 100 μg/mL could promote damage repair and effectively inhibit the release of LDH production in IPEC-J2 cells after exposure to H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub>. Additionally, it had a restorative effect on the loss of TrxR, SOD, GSH, and CAT. At the same time, the treatment of CHS-IVa significantly decreased the mRNA expression of NF-κB, IL-6, and P65 after H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> treatment. Conclusion: These findings indicate that a specific concentration of CHS-IVa has a protective effect on IPEC during oxidative stress, thereby enhancing their ability to repair damage.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"41 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141778610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-25DOI: 10.1177/1934578x241258290
Karin Valant-Vetschera, Lothar Brecker, Johann Schinnerl
Flavone is a very simple and unsubstituted flavonoid of restricted distribution in the plant kingdom. Historically it is known for a long time as major constituent of many Primula and Dionysia exudates produced by glandular hairs in unusually large amounts, often as a farinose (mealy) deposit on aerial plant parts. The ecological function of this unique farinose deposit and its main compound flavone is still not really understood. Concerning flavone, a knowledge gap exists about its biosynthetic origin, when compared to all of the flavonoid biosynthetic routes known to date. Thus, this review addresses not only the history of this compound and its occurrence in plants and in their respective compartments, but also discusses ideas on its putative biosynthesis. Furthermore, we provide up-to-date analytical data on this compound from a natural source, rather than from chemosynthesis, in a comprehensive way. Eco-functional aspects complement this study that is intended to be highly stimulating for future research in ecology and evolution.
{"title":"Flavone From Plants—Uncommonly Common? A Comprehensive Review","authors":"Karin Valant-Vetschera, Lothar Brecker, Johann Schinnerl","doi":"10.1177/1934578x241258290","DOIUrl":"https://doi.org/10.1177/1934578x241258290","url":null,"abstract":"Flavone is a very simple and unsubstituted flavonoid of restricted distribution in the plant kingdom. Historically it is known for a long time as major constituent of many Primula and Dionysia exudates produced by glandular hairs in unusually large amounts, often as a farinose (mealy) deposit on aerial plant parts. The ecological function of this unique farinose deposit and its main compound flavone is still not really understood. Concerning flavone, a knowledge gap exists about its biosynthetic origin, when compared to all of the flavonoid biosynthetic routes known to date. Thus, this review addresses not only the history of this compound and its occurrence in plants and in their respective compartments, but also discusses ideas on its putative biosynthesis. Furthermore, we provide up-to-date analytical data on this compound from a natural source, rather than from chemosynthesis, in a comprehensive way. Eco-functional aspects complement this study that is intended to be highly stimulating for future research in ecology and evolution.","PeriodicalId":19019,"journal":{"name":"Natural Product Communications","volume":"69 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141778605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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