Pub Date : 2025-11-11DOI: 10.1038/s41580-025-00930-4
Mengxi Zhang
In this Tools of the Trade article, Zhang (Xiao Lab) highlights the development of a rotor-based fluorescent amino acid that acts as a sensor for crowded protein microenvironments, enabling researchers to monitor protein behaviour in vivo.
{"title":"Illuminating protein microenvironments with rotor-based fluorescent amino acids","authors":"Mengxi Zhang","doi":"10.1038/s41580-025-00930-4","DOIUrl":"10.1038/s41580-025-00930-4","url":null,"abstract":"In this Tools of the Trade article, Zhang (Xiao Lab) highlights the development of a rotor-based fluorescent amino acid that acts as a sensor for crowded protein microenvironments, enabling researchers to monitor protein behaviour in vivo.","PeriodicalId":19051,"journal":{"name":"Nature Reviews Molecular Cell Biology","volume":"27 2","pages":"92-92"},"PeriodicalIF":90.2,"publicationDate":"2025-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145484713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-11DOI: 10.1038/s41580-025-00917-1
Sushant Bangru,Rocky Diegmiller,Stefano Di Talia,Kenneth D Poss
Tissue regeneration has historically been the subject of intense scientific scrutiny, from basic biology to applications in regenerative medicine. Use of model organisms and cutting-edge technologies have uncovered various mechanisms of regeneration, but understanding how signals are regulated spatiotemporally to renew lost structures at scale remains a challenge. Recent insights into chromatin structure and enhancer regulation, immune-tissue crosstalk, bioelectric and metabolic cues and quantitative modelling are broadening and reshaping our understanding of how tissues repair and renew. The evolution of cutting-edge tools for in vivo profiling and tracking of single cells is providing unprecedented dynamic views of regeneration across scales. Here, we synthesize the current knowledge of signal control in regeneration, with emphasis on conceptual advances, technical innovations and future directions for a more quantitative understanding of regenerative biology.
{"title":"Signal control during tissue regeneration in adult animals.","authors":"Sushant Bangru,Rocky Diegmiller,Stefano Di Talia,Kenneth D Poss","doi":"10.1038/s41580-025-00917-1","DOIUrl":"https://doi.org/10.1038/s41580-025-00917-1","url":null,"abstract":"Tissue regeneration has historically been the subject of intense scientific scrutiny, from basic biology to applications in regenerative medicine. Use of model organisms and cutting-edge technologies have uncovered various mechanisms of regeneration, but understanding how signals are regulated spatiotemporally to renew lost structures at scale remains a challenge. Recent insights into chromatin structure and enhancer regulation, immune-tissue crosstalk, bioelectric and metabolic cues and quantitative modelling are broadening and reshaping our understanding of how tissues repair and renew. The evolution of cutting-edge tools for in vivo profiling and tracking of single cells is providing unprecedented dynamic views of regeneration across scales. Here, we synthesize the current knowledge of signal control in regeneration, with emphasis on conceptual advances, technical innovations and future directions for a more quantitative understanding of regenerative biology.","PeriodicalId":19051,"journal":{"name":"Nature Reviews Molecular Cell Biology","volume":"54 1","pages":""},"PeriodicalIF":112.7,"publicationDate":"2025-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145491606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-10DOI: 10.1038/s41580-025-00907-3
Emma T. Watson, William R. Wegeng, Stamatina Aravani, Andreas M. Ernst, Julia von Blume
The Golgi apparatus has a central role in the formation and trafficking of glycoproteins and lipids. It is organized into a series of flattened, membrane-bound compartments called cisternae, each housing a unique set of resident proteins that sequentially modify newly synthesized proteins and lipids as they move through the Golgi stack. In the final compartments, known as the trans-Golgi network (TGN), the processed cargoes are sorted and packaged into transport carriers. Despite substantial progress, key questions remain about how proteins and lipids are selectively sorted within the Golgi for delivery to specific destinations. In this Review we highlight recent insights on the biogenesis of membrane carriers at the TGN that enable transport of macromolecules along the secretory pathway and discuss how dysfunction of the molecular machinery gives rise to Golgi-related diseases. Protein and lipid cargoes are modified and sorted in the Golgi apparatus and packaged for delivery to diverse cellular destinations at the trans-Golgi network (TGN). This Review discusses recent insights into Golgi transport mechanisms and carrier biogenesis at the TGN.
{"title":"Mechanistic insights into cargo sorting and export from the Golgi apparatus","authors":"Emma T. Watson, William R. Wegeng, Stamatina Aravani, Andreas M. Ernst, Julia von Blume","doi":"10.1038/s41580-025-00907-3","DOIUrl":"10.1038/s41580-025-00907-3","url":null,"abstract":"The Golgi apparatus has a central role in the formation and trafficking of glycoproteins and lipids. It is organized into a series of flattened, membrane-bound compartments called cisternae, each housing a unique set of resident proteins that sequentially modify newly synthesized proteins and lipids as they move through the Golgi stack. In the final compartments, known as the trans-Golgi network (TGN), the processed cargoes are sorted and packaged into transport carriers. Despite substantial progress, key questions remain about how proteins and lipids are selectively sorted within the Golgi for delivery to specific destinations. In this Review we highlight recent insights on the biogenesis of membrane carriers at the TGN that enable transport of macromolecules along the secretory pathway and discuss how dysfunction of the molecular machinery gives rise to Golgi-related diseases. Protein and lipid cargoes are modified and sorted in the Golgi apparatus and packaged for delivery to diverse cellular destinations at the trans-Golgi network (TGN). This Review discusses recent insights into Golgi transport mechanisms and carrier biogenesis at the TGN.","PeriodicalId":19051,"journal":{"name":"Nature Reviews Molecular Cell Biology","volume":"26 12","pages":"940-956"},"PeriodicalIF":90.2,"publicationDate":"2025-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145478132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-06DOI: 10.1038/s41580-025-00914-4
Weili Miao, Douglas F. Porter, Vanessa Lopez-Pajares, Paul A. Khavari
{"title":"Regulation of RNA-binding proteins by small biomolecules","authors":"Weili Miao, Douglas F. Porter, Vanessa Lopez-Pajares, Paul A. Khavari","doi":"10.1038/s41580-025-00914-4","DOIUrl":"https://doi.org/10.1038/s41580-025-00914-4","url":null,"abstract":"","PeriodicalId":19051,"journal":{"name":"Nature Reviews Molecular Cell Biology","volume":"30 1","pages":""},"PeriodicalIF":112.7,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145447223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-06DOI: 10.1038/s41580-025-00927-z
Barbara Demmig-Adams
{"title":"First molecular evidence for zeaxanthin’s role in energy dissipation","authors":"Barbara Demmig-Adams","doi":"10.1038/s41580-025-00927-z","DOIUrl":"https://doi.org/10.1038/s41580-025-00927-z","url":null,"abstract":"","PeriodicalId":19051,"journal":{"name":"Nature Reviews Molecular Cell Biology","volume":"1 1","pages":""},"PeriodicalIF":112.7,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145455366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-05DOI: 10.1038/s41580-025-00929-x
Lisa Heinke
A study finds that mechanical activation leads to microtubule rearrangement, facilitating the proteasomal degradation of an inhibitor of YAP/TAZ, thus promoting YAP/TAZ nuclear translocation.
{"title":"Microtubules provide a mechanical link for YAP/TAZ signalling","authors":"Lisa Heinke","doi":"10.1038/s41580-025-00929-x","DOIUrl":"10.1038/s41580-025-00929-x","url":null,"abstract":"A study finds that mechanical activation leads to microtubule rearrangement, facilitating the proteasomal degradation of an inhibitor of YAP/TAZ, thus promoting YAP/TAZ nuclear translocation.","PeriodicalId":19051,"journal":{"name":"Nature Reviews Molecular Cell Biology","volume":"26 12","pages":"903-903"},"PeriodicalIF":90.2,"publicationDate":"2025-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145440939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-05DOI: 10.1038/s41580-025-00926-0
Crisanto Gutierrez
{"title":"Discovery of a human gene encoding a cyclin-dependent kinase","authors":"Crisanto Gutierrez","doi":"10.1038/s41580-025-00926-0","DOIUrl":"https://doi.org/10.1038/s41580-025-00926-0","url":null,"abstract":"","PeriodicalId":19051,"journal":{"name":"Nature Reviews Molecular Cell Biology","volume":"38 1","pages":""},"PeriodicalIF":112.7,"publicationDate":"2025-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145440936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-03DOI: 10.1038/s41580-025-00920-6
Fabricio Loayza-Puch
The discovery of noncanonical short open reading frames that produce functional microproteins upended our perception of large parts of our genome as ‘noncoding’.
产生功能性微蛋白的非规范短开放阅读框的发现颠覆了我们对大部分基因组为“非编码”的看法。
{"title":"Revealing the hidden coding potential of the human genome","authors":"Fabricio Loayza-Puch","doi":"10.1038/s41580-025-00920-6","DOIUrl":"10.1038/s41580-025-00920-6","url":null,"abstract":"The discovery of noncanonical short open reading frames that produce functional microproteins upended our perception of large parts of our genome as ‘noncoding’.","PeriodicalId":19051,"journal":{"name":"Nature Reviews Molecular Cell Biology","volume":"27 2","pages":"93-93"},"PeriodicalIF":90.2,"publicationDate":"2025-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145427582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-03DOI: 10.1038/s41580-025-00923-3
Drishan Dahal
In this Tools of the Trade article, Dahal (Rehling lab) discusses the development of a polymorpholino oligonucleotide chimera tool that enables selective and efficient silencing of mitochondrial mRNA.
{"title":"Silencing of mitochondrial gene expression using polymorpholino chimeras","authors":"Drishan Dahal","doi":"10.1038/s41580-025-00923-3","DOIUrl":"10.1038/s41580-025-00923-3","url":null,"abstract":"In this Tools of the Trade article, Dahal (Rehling lab) discusses the development of a polymorpholino oligonucleotide chimera tool that enables selective and efficient silencing of mitochondrial mRNA.","PeriodicalId":19051,"journal":{"name":"Nature Reviews Molecular Cell Biology","volume":"27 1","pages":"8-8"},"PeriodicalIF":90.2,"publicationDate":"2025-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145427581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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