Pub Date : 2021-09-25DOI: 10.26641/1997-9665.2020.4.64-71
I. S. Sobolevskaya, M. I. Krasnobaeva, O. D. Myadelets
Background. It has now been established that any change in the synchronized work of the circadian clock leads to a disruption of the body's regulatory systems, which consists in the development of metabolic disorders and tissue damage. Objective: to study the dynamics of the expression of the MT1 melatonin receptor in the skin of white male rats under light deprivation. Methods. In this work, using immunohistochemistry, the features of the expression of receptors for melatonin (MT1) were studied. The experiments were performed on outbred male rats weighing 170-220 g, which were divided into 2 groups; intact - animals kept under standard fixed lighting conditions (12 h light / 12 h dark); Group 2 - animals with simulated light deprivation in conditions of round-the-clock darkness (24 hours of darkness). Results. A strong circadian dependence of the expression of MT1 melatonin receptors in the general integument was established. In the cells of the epidermis and sebaceous glands, by the 14th day, MT1 expression significantly decreased, which then sharply increased by the 21st day. In the keratinocytes of the epidermis, a wavelike character of changes in indicators is noted throughout the study: an increase (7th day) - a decrease (14th day) - a sharp increase (21st day). Conclusion. Initially, light deprivation leads to a decrease in the number of immunopositive cells, and then their level increases compensatory, which is one of the manifestations of the body's adaptation mechanisms to chronodestruction.
{"title":"Features of the expression of melatonin MT1 receptors in the general cover of rats caused by light deprivation","authors":"I. S. Sobolevskaya, M. I. Krasnobaeva, O. D. Myadelets","doi":"10.26641/1997-9665.2020.4.64-71","DOIUrl":"https://doi.org/10.26641/1997-9665.2020.4.64-71","url":null,"abstract":"Background. It has now been established that any change in the synchronized work of the circadian clock leads to a disruption of the body's regulatory systems, which consists in the development of metabolic disorders and tissue damage. Objective: to study the dynamics of the expression of the MT1 melatonin receptor in the skin of white male rats under light deprivation. Methods. In this work, using immunohistochemistry, the features of the expression of receptors for melatonin (MT1) were studied. The experiments were performed on outbred male rats weighing 170-220 g, which were divided into 2 groups; intact - animals kept under standard fixed lighting conditions (12 h light / 12 h dark); Group 2 - animals with simulated light deprivation in conditions of round-the-clock darkness (24 hours of darkness). Results. A strong circadian dependence of the expression of MT1 melatonin receptors in the general integument was established. In the cells of the epidermis and sebaceous glands, by the 14th day, MT1 expression significantly decreased, which then sharply increased by the 21st day. In the keratinocytes of the epidermis, a wavelike character of changes in indicators is noted throughout the study: an increase (7th day) - a decrease (14th day) - a sharp increase (21st day). Conclusion. Initially, light deprivation leads to a decrease in the number of immunopositive cells, and then their level increases compensatory, which is one of the manifestations of the body's adaptation mechanisms to chronodestruction.","PeriodicalId":19107,"journal":{"name":"Morphologia","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87366749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-25DOI: 10.26641/1997-9665.2020.4.79-89
I. Tverdokhlib, D. Zinenko
Background. The development of acute pancreatitis is not limited to isolated damage to the pancreas. After creating models of acute pancreatitis using various substances that enhance the secretion of the gland, have a toxic or local activating effect, the researchers showed their dose-dependent effect. The question of the reaction of the hepatic microcirculation system during the development of acute pancreatitis, as well as their pathogenetic significance in the development of pathomorphological changes in the pancreas and liver in most aspects remains open. Objective. The purpose of the current study was to define the role of the hepatic mircocirculation in development of ultrastructural parenchymatous-stromal changes of the pancreas and liver in a model of acute pancreatitis using different doses of L-arginin. Methods. The variants of acute pancreatitis model were used with injection of L-arginin in dosage 3 g/kg; 4 g/kg and 5 g/kg. The morphological research of pancreas and liver were carried out in 1, 4, 8, 12, 24, 48 and 72 hours after initiation of inflammation. Results. The visible reaction of hepatic mircocirculation in the experimental model of acute pancreatitis was depended on character of pathomorphological changes in pancreas. This reaction demonstrated the phase character including: 1) activation of hepatic circulation, first of all in portal component, against a background of pancreatic enzyme toxemia; 2) development of inflammatory, dystrophic, destructive and necrotic changes in hepatic parenchyme together with mircocirculation disorders against a background of pancreatic necrotic toxemia; 3) recovery and adaptation or decompensation processes in mircocirculation system of liver and hepatic parenchyme depending on the degree of pancreatogenic toxemia|. Conclusion. Within 72 hours of the experiment, at the lowest and middling doses of L-arginin, in the context of reduction of acute pancreatitis, there is a gradual renovation of the structure of the microvessels and normalization of the microcirculation of the liver. In the maximum doses L-arginin cause degradation of the liver microvessels with the progression of hemorrhages, slit red blood cells and platelet aggregation, which causes blockage of the microcirculation and the development of necrotic changes in the hepatic parenchyma.
{"title":"Ultrastructure of the hemomicrocirculatory bed and parenchymatous-stromal elements of the pancreas and liver in a model of acute pancreatitis using different doses of L-arginin","authors":"I. Tverdokhlib, D. Zinenko","doi":"10.26641/1997-9665.2020.4.79-89","DOIUrl":"https://doi.org/10.26641/1997-9665.2020.4.79-89","url":null,"abstract":"Background. The development of acute pancreatitis is not limited to isolated damage to the pancreas. After creating models of acute pancreatitis using various substances that enhance the secretion of the gland, have a toxic or local activating effect, the researchers showed their dose-dependent effect. The question of the reaction of the hepatic microcirculation system during the development of acute pancreatitis, as well as their pathogenetic significance in the development of pathomorphological changes in the pancreas and liver in most aspects remains open. Objective. The purpose of the current study was to define the role of the hepatic mircocirculation in development of ultrastructural parenchymatous-stromal changes of the pancreas and liver in a model of acute pancreatitis using different doses of L-arginin. Methods. The variants of acute pancreatitis model were used with injection of L-arginin in dosage 3 g/kg; 4 g/kg and 5 g/kg. The morphological research of pancreas and liver were carried out in 1, 4, 8, 12, 24, 48 and 72 hours after initiation of inflammation. Results. The visible reaction of hepatic mircocirculation in the experimental model of acute pancreatitis was depended on character of pathomorphological changes in pancreas. This reaction demonstrated the phase character including: 1) activation of hepatic circulation, first of all in portal component, against a background of pancreatic enzyme toxemia; 2) development of inflammatory, dystrophic, destructive and necrotic changes in hepatic parenchyme together with mircocirculation disorders against a background of pancreatic necrotic toxemia; 3) recovery and adaptation or decompensation processes in mircocirculation system of liver and hepatic parenchyme depending on the degree of pancreatogenic toxemia|. Conclusion. Within 72 hours of the experiment, at the lowest and middling doses of L-arginin, in the context of reduction of acute pancreatitis, there is a gradual renovation of the structure of the microvessels and normalization of the microcirculation of the liver. In the maximum doses L-arginin cause degradation of the liver microvessels with the progression of hemorrhages, slit red blood cells and platelet aggregation, which causes blockage of the microcirculation and the development of necrotic changes in the hepatic parenchyma.","PeriodicalId":19107,"journal":{"name":"Morphologia","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91011036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-25DOI: 10.26641/1997-9665.2020.4.120-121
Б. И. Яремин, А. А. Супильников, Н. А. Лысов
НекрологПрофессор Валентина Дмитриевна ИвановаПрофессор Валентина Дмитриевна Иванова (1927-2020) является подлинной легендой российской морфологической и хирургической школы, её гордостью и достоянием. В 1944 году Валентина Дмитриевна поступила в Куйбышевский медицинский институт, а в 1946 переведена в Кишиневский медицинский институт, который окончила в 1949 г. По окончании ординатуры уезжает за мужем-офицером в город Холмск. Начав работать врачом-хирургом, через год Валентина Дмитриевна в возрасте 25 лет становится заведующей хирургическим отделением Холмской городской больницы в Сахалинской области и главным хирургом г. Холмска. Там она осваивает неотложную хирургию органов брюшной полости, гинекологическую хирургию и в 1954 году награждается знаком «отличник здравоохранения СССР». В должности заведующего отделением Валентина Дмитриевна оперирует известного певца Вадима Козина, оказавшегося в Холмске с флегмонозным аппендицитом. В 1955-1957 гг. проходит обучение на кафедре академика АМН СССР генерала Петра Андреевича Куприянова в Ленинграде. Именно в этом коллективе сформировались научные предпочтения Валентины Дмитриевны – сердечно-сосудистая хирургия, вопросы гемодинамики в неотложной медицине и интенсивной терапии. Продолжая эти направления, в 1957 году Валентина Дмитриевна возвращается на родину в аспирантуру на кафедру факультетской хирургии Куйбышевского медицинского института, которую возглавлял один из основоположников грудной хирургии в нашей стране и ученик П.А. Купирянова профессор С. Л. Либов. Через три года Валентина Дмитриевна успешно защитила кандидатскую диссертацию на тему «Некоторые вопросы хирургического лечения хронической коронарной недостаточности» в Горьковском медицинском институте. В связи с вынужденным отъездом С.Л. Либова из Куйбышева Валентина Дмитриевна способствует переезду на кафедру перспективного сосудистого хирурга Г.Л. Ратнера, в последствии ставшего основоположником куйбышевской научно-педагогической школы хирургов. Кафедре факультетской хирургии Валентина Дмитриевна отдала 11 лет, работала во всех хирургических отделениях, заведовала общехирургическим отделением. В 1971 году защитила докторскую диссертацию на тему «Эффективность экстракорпорального кровообращения при лечении терминальных состояний с гипотензией». Эта работа является фундаментальным экспериментально-клиническим исследованием, направленным на изучение вариантов перфузии внутренних органов человека при проведении баллонной контрпульсации, экстракорпорального кровообращения. В 1974 году переведена на должность заведующей кафедрой оперативной хирургии и топографической анатомии Куйбышевского медицинского института, кафедры, которая стала делом жизни Валентины Дмитриевны. В этой должности Валентина Дмитриевна проработала 25 лет, совершенствуя педагогическую, воспитательную работу на кафедре, вносила новое в оптимизацию и методику преподавания оперативной хирургии и топографической анатомии. Валентина Дмитриевна сформировала и возглавила яркий т
{"title":"News, chronicle, history","authors":"Б. И. Яремин, А. А. Супильников, Н. А. Лысов","doi":"10.26641/1997-9665.2020.4.120-121","DOIUrl":"https://doi.org/10.26641/1997-9665.2020.4.120-121","url":null,"abstract":"НекрологПрофессор Валентина Дмитриевна ИвановаПрофессор Валентина Дмитриевна Иванова (1927-2020) является подлинной легендой российской морфологической и хирургической школы, её гордостью и достоянием. В 1944 году Валентина Дмитриевна поступила в Куйбышевский медицинский институт, а в 1946 переведена в Кишиневский медицинский институт, который окончила в 1949 г. По окончании ординатуры уезжает за мужем-офицером в город Холмск. Начав работать врачом-хирургом, через год Валентина Дмитриевна в возрасте 25 лет становится заведующей хирургическим отделением Холмской городской больницы в Сахалинской области и главным хирургом г. Холмска. Там она осваивает неотложную хирургию органов брюшной полости, гинекологическую хирургию и в 1954 году награждается знаком «отличник здравоохранения СССР». В должности заведующего отделением Валентина Дмитриевна оперирует известного певца Вадима Козина, оказавшегося в Холмске с флегмонозным аппендицитом. В 1955-1957 гг. проходит обучение на кафедре академика АМН СССР генерала Петра Андреевича Куприянова в Ленинграде. Именно в этом коллективе сформировались научные предпочтения Валентины Дмитриевны – сердечно-сосудистая хирургия, вопросы гемодинамики в неотложной медицине и интенсивной терапии. Продолжая эти направления, в 1957 году Валентина Дмитриевна возвращается на родину в аспирантуру на кафедру факультетской хирургии Куйбышевского медицинского института, которую возглавлял один из основоположников грудной хирургии в нашей стране и ученик П.А. Купирянова профессор С. Л. Либов. Через три года Валентина Дмитриевна успешно защитила кандидатскую диссертацию на тему «Некоторые вопросы хирургического лечения хронической коронарной недостаточности» в Горьковском медицинском институте. В связи с вынужденным отъездом С.Л. Либова из Куйбышева Валентина Дмитриевна способствует переезду на кафедру перспективного сосудистого хирурга Г.Л. Ратнера, в последствии ставшего основоположником куйбышевской научно-педагогической школы хирургов. Кафедре факультетской хирургии Валентина Дмитриевна отдала 11 лет, работала во всех хирургических отделениях, заведовала общехирургическим отделением. В 1971 году защитила докторскую диссертацию на тему «Эффективность экстракорпорального кровообращения при лечении терминальных состояний с гипотензией». Эта работа является фундаментальным экспериментально-клиническим исследованием, направленным на изучение вариантов перфузии внутренних органов человека при проведении баллонной контрпульсации, экстракорпорального кровообращения. В 1974 году переведена на должность заведующей кафедрой оперативной хирургии и топографической анатомии Куйбышевского медицинского института, кафедры, которая стала делом жизни Валентины Дмитриевны. В этой должности Валентина Дмитриевна проработала 25 лет, совершенствуя педагогическую, воспитательную работу на кафедре, вносила новое в оптимизацию и методику преподавания оперативной хирургии и топографической анатомии. Валентина Дмитриевна сформировала и возглавила яркий т","PeriodicalId":19107,"journal":{"name":"Morphologia","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89790164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-25DOI: 10.26641/1997-9665.2020.4.101-107
J. Musayev, N. Metilli, R. Sholan, A. Hasanov, A. Damirli, K. Bakhshaliyeva
Background. Urine cytology is deemed a sensitive method in detection of high-grade urothelial carcinoma. In contrast, detection of low-grade urothelial carcinoma (LGUC) and its differentiation from reactive lesions is difficult with urinary cytology. Objective. Our study aims to determine the effectiveness of morphometric parameters in differentiating reactive urothelial cells from LGUC by cytological examination of urine specimens. Methods. Voided urine samples were used for the study, while the cases were randomized into two groups: those diagnosed with LGUC (first group; N=10) and those which were not diagnosed with LGUC (second group; N=10). The morphometric parameters of major nuclear diameter (MaND), minor nuclear diameter (MiND), mean nuclear area (MNA), cell diameter (CD), mean cell area (MCA), as well as MaND/CD, MiND/CD, MiND/MaND and MNA/MCA ratios were measured on 100 urothelial cells for each case through ScopeImage® 9.0 software. Results. A statistically significant difference was found between the mean values of MiND/CD (p=0.017) and MNA/MCA (p=0.002) ratios of groups. The mean value of both parameters in the first group constituted 0.2 and higher, and below 0.2 in the second group. Conclusion. The ratios of MiND/CD and MNA/MCA in urothelial cells proved significantly higher in patients with LGUC than benign/reactive cases. The reliability of these findings in differentiating LGUC from benign/reactive lesions needs to be verified through studies examining a large number of cases. These parameters can be assessed much faster through a special software enabling an automatic measurement and thus can be used in routine cytological examination.
{"title":"The importance of morphometric parameters in differentiating benign/reactive urothelial cells from low-grade urothelial carcinoma: computer-assisted study on urine specimens","authors":"J. Musayev, N. Metilli, R. Sholan, A. Hasanov, A. Damirli, K. Bakhshaliyeva","doi":"10.26641/1997-9665.2020.4.101-107","DOIUrl":"https://doi.org/10.26641/1997-9665.2020.4.101-107","url":null,"abstract":"Background. Urine cytology is deemed a sensitive method in detection of high-grade urothelial carcinoma. In contrast, detection of low-grade urothelial carcinoma (LGUC) and its differentiation from reactive lesions is difficult with urinary cytology. Objective. Our study aims to determine the effectiveness of morphometric parameters in differentiating reactive urothelial cells from LGUC by cytological examination of urine specimens. Methods. Voided urine samples were used for the study, while the cases were randomized into two groups: those diagnosed with LGUC (first group; N=10) and those which were not diagnosed with LGUC (second group; N=10). The morphometric parameters of major nuclear diameter (MaND), minor nuclear diameter (MiND), mean nuclear area (MNA), cell diameter (CD), mean cell area (MCA), as well as MaND/CD, MiND/CD, MiND/MaND and MNA/MCA ratios were measured on 100 urothelial cells for each case through ScopeImage® 9.0 software. Results. A statistically significant difference was found between the mean values of MiND/CD (p=0.017) and MNA/MCA (p=0.002) ratios of groups. The mean value of both parameters in the first group constituted 0.2 and higher, and below 0.2 in the second group. Conclusion. The ratios of MiND/CD and MNA/MCA in urothelial cells proved significantly higher in patients with LGUC than benign/reactive cases. The reliability of these findings in differentiating LGUC from benign/reactive lesions needs to be verified through studies examining a large number of cases. These parameters can be assessed much faster through a special software enabling an automatic measurement and thus can be used in routine cytological examination.","PeriodicalId":19107,"journal":{"name":"Morphologia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89732223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-25DOI: 10.26641/1997-9665.2020.4.95-100
O. I. Zalyubovska, N. О. Hladkykh
Background. Thyroid cancer has long been an urgent problem of health care in Ukraine and requires both improved timely detection of the disease and increased coverage by special treatment of this category of patients to improve its results. Objective. Investigate the complex of immunocytochemical and ultrasound characteristics of thyroid tumors to improve diagnostic algorithms in the preanalytical stage. Methods. During the the research work the results of diagnostics of 60 patients with thyroid neoplasms aged 21 to 83 years who underwent fine-needle aspiration. Patients were divided into three groups according to the Bethesda System (BSRTC) classification category. The 1st group included 18 patients of category II BSRTC (Benign. Group 2 included 20 patients with category V BSRTC (Suspicious for Malignancy – SFM). And other 22 patients with category VI BSRTC (malignant tumor) formed the 3rd group). Patients of all groups underwent questionnaires, ultrasound examination with FNA, cytological and immunocytochemical examination. Results. There is no clear link between E-cadherin expression and neoplasia. Negative expression occurred in 36.4% of cases of metastatic lesions. This partially satisfies the requirements of timely preoperative diagnosis. That is why E-cadherin cannot be used as a separate marker. The expression of cytokeratin-19 with different degrees of intensity of cytoplasmic staining of tumor cells was determined in all studied neoplasms of V and VI category on the BSRTC scale. Strong expression of cytokeratin-19 was observed in 40.0% of cases with BSRTC-5 and 45.4% with BSRTC-6, moderate - in 50.0% and 36.4% of cases, respectively, weak - in 10.0% and 18.2%, without significant differences between groups (p> 0.05). At the same time, in the benign process in 55.6% of cases there was no reaction of cytokeratin-19, and in 44.4% - weak expression (p <0.001 in comparison with the 2nd and 3rd groups). Conclusion. Benign thyroid disease (category II BSRTC) is likely to be associated with younger patients (46.6 ± 3.7 years), low serum REA (less than 0.95 ng / ml), poor thyroglobulin expression (77.8 %), negative reaction with TTF-1 (100%) and cytokeratin-19 (55.6%). Differential-prognostic markers of thyroid cancer with risk of malignancy (V category BSRTC) include elevated serum REA (0.95 ng / ml and above), the presence of a moderate reaction with antibodies to thyroglobulin (80.0%), a positive reaction - to TTF-1 (100.0%) and E-cadherin (90.0%), with moderate or strong expression of cytokeratin-19 (90.0%).
背景。甲状腺癌长期以来一直是乌克兰医疗保健的一个紧迫问题，需要更好地及时发现这种疾病，并通过对这类患者的特殊治疗来扩大覆盖面，以改善治疗效果。目标。探讨甲状腺肿瘤的免疫细胞化学和超声特征的复合体，以改进分析前阶段的诊断算法。方法。本文对60例21 ~ 83岁甲状腺肿瘤患者行细针穿刺的诊断结果进行了分析。根据Bethesda系统(BSRTC)分类将患者分为三组。第一组包括18例II型BSRTC (Benign;第二组包括20例V型BSRTC(可疑恶性肿瘤- SFM)患者。第三组为VI类BSRTC(恶性肿瘤)患者22例。各组患者均进行问卷调查、FNA超声检查、细胞学及免疫细胞化学检查。结果。e -钙粘蛋白的表达与肿瘤之间没有明确的联系。36.4%的转移灶出现阴性表达。这部分满足了术前及时诊断的要求。这就是为什么e -钙粘蛋白不能作为单独的标记物。采用BSRTC分级法，检测V、VI类肿瘤细胞中细胞角蛋白-19在不同细胞质染色强度下的表达。BSRTC-5和BSRTC-6患者中细胞角蛋白19的表达分别为40.0%和45.4%，中度表达分别为50.0%和36.4%，弱表达分别为10.0%和18.2%，组间差异无统计学意义(p> 0.05)。同时，55.6%的病例良性过程中细胞角蛋白-19无反应，44.4%的病例弱表达(与2、3组比较p <0.001)。结论。良性甲状腺疾病(II类BSRTC)可能与患者年轻化(46.6±3.7岁)、血清REA低(小于0.95 ng / ml)、甲状腺球蛋白表达差(77.8%)、TTF-1阴性(100%)和细胞角蛋白-19阴性(55.6%)相关。具有恶性肿瘤风险的甲状腺癌(V类BSRTC)的鉴别预后指标包括血清REA升高(0.95 ng / ml及以上)，甲状腺球蛋白抗体存在中度反应(80.0%)，TTF-1和E-cadherin阳性反应(100.0%)，细胞角蛋白19中度或强烈表达(90.0%)。
{"title":"The feasibility of using immunocytochemical research in the preoperative diagnosis of thyroid tumors","authors":"O. I. Zalyubovska, N. О. Hladkykh","doi":"10.26641/1997-9665.2020.4.95-100","DOIUrl":"https://doi.org/10.26641/1997-9665.2020.4.95-100","url":null,"abstract":"Background. Thyroid cancer has long been an urgent problem of health care in Ukraine and requires both improved timely detection of the disease and increased coverage by special treatment of this category of patients to improve its results. Objective. Investigate the complex of immunocytochemical and ultrasound characteristics of thyroid tumors to improve diagnostic algorithms in the preanalytical stage. Methods. During the the research work the results of diagnostics of 60 patients with thyroid neoplasms aged 21 to 83 years who underwent fine-needle aspiration. Patients were divided into three groups according to the Bethesda System (BSRTC) classification category. The 1st group included 18 patients of category II BSRTC (Benign. Group 2 included 20 patients with category V BSRTC (Suspicious for Malignancy – SFM). And other 22 patients with category VI BSRTC (malignant tumor) formed the 3rd group). Patients of all groups underwent questionnaires, ultrasound examination with FNA, cytological and immunocytochemical examination. Results. There is no clear link between E-cadherin expression and neoplasia. Negative expression occurred in 36.4% of cases of metastatic lesions. This partially satisfies the requirements of timely preoperative diagnosis. That is why E-cadherin cannot be used as a separate marker. The expression of cytokeratin-19 with different degrees of intensity of cytoplasmic staining of tumor cells was determined in all studied neoplasms of V and VI category on the BSRTC scale. Strong expression of cytokeratin-19 was observed in 40.0% of cases with BSRTC-5 and 45.4% with BSRTC-6, moderate - in 50.0% and 36.4% of cases, respectively, weak - in 10.0% and 18.2%, without significant differences between groups (p> 0.05). At the same time, in the benign process in 55.6% of cases there was no reaction of cytokeratin-19, and in 44.4% - weak expression (p <0.001 in comparison with the 2nd and 3rd groups). Conclusion. Benign thyroid disease (category II BSRTC) is likely to be associated with younger patients (46.6 ± 3.7 years), low serum REA (less than 0.95 ng / ml), poor thyroglobulin expression (77.8 %), negative reaction with TTF-1 (100%) and cytokeratin-19 (55.6%). Differential-prognostic markers of thyroid cancer with risk of malignancy (V category BSRTC) include elevated serum REA (0.95 ng / ml and above), the presence of a moderate reaction with antibodies to thyroglobulin (80.0%), a positive reaction - to TTF-1 (100.0%) and E-cadherin (90.0%), with moderate or strong expression of cytokeratin-19 (90.0%).","PeriodicalId":19107,"journal":{"name":"Morphologia","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90052762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-25DOI: 10.26641/1997-9665.2020.4.90-94
E. Grygorian, M. Myroshnychenko
Background. Assessing the postmortem interval (PMI) is one of the most problematic issues in judicial practice. Objective. To investigate the morphological changes of uterus tissues to determine the PMI-dependent features of postmortem changes development. Methods. A morphological analysis of 40 uterus tissue samples was performed; consisting of two groups: first group – samples from the corpses of women aged 23 to 70 years (n = 34), second group – comparison group – biopsy material from 6 women with uterine prolapse, uterine leiomyoma, from unaffected areas. Results. In the period from 24 to 48 hours after death, there were changes in the form of a slight change in the structure of cellular elements, changes in their color, the presence of light gaps between connective tissue and muscle fibers. In the period from 48 to 72 hours, the destruction of the border between the muscular and serous membranes, blurred contours and ruptures of cells, severe swelling of muscle fibers, wide gaps between muscle fibers, a significant decrease in fibroblastic cells were observed. In addition, there was no endothelium in a significant number of vessels, loss of clarity of fiber bundles, lack of nuclei in a significant number of myocytes. Desquamed endothelial cells and hemolyzed erythrocytes were seen in the openings of most vessels. Conclusion. Histological examination of uterine tissues showed the presence of specific changes during the studied time intervals after death, which can be used to introduce criteria to determine the time since death in practice.
{"title":"Features of morphological changes of the uterus after the death prescription","authors":"E. Grygorian, M. Myroshnychenko","doi":"10.26641/1997-9665.2020.4.90-94","DOIUrl":"https://doi.org/10.26641/1997-9665.2020.4.90-94","url":null,"abstract":"Background. Assessing the postmortem interval (PMI) is one of the most problematic issues in judicial practice. Objective. To investigate the morphological changes of uterus tissues to determine the PMI-dependent features of postmortem changes development. Methods. A morphological analysis of 40 uterus tissue samples was performed; consisting of two groups: first group – samples from the corpses of women aged 23 to 70 years (n = 34), second group – comparison group – biopsy material from 6 women with uterine prolapse, uterine leiomyoma, from unaffected areas. Results. In the period from 24 to 48 hours after death, there were changes in the form of a slight change in the structure of cellular elements, changes in their color, the presence of light gaps between connective tissue and muscle fibers. In the period from 48 to 72 hours, the destruction of the border between the muscular and serous membranes, blurred contours and ruptures of cells, severe swelling of muscle fibers, wide gaps between muscle fibers, a significant decrease in fibroblastic cells were observed. In addition, there was no endothelium in a significant number of vessels, loss of clarity of fiber bundles, lack of nuclei in a significant number of myocytes. Desquamed endothelial cells and hemolyzed erythrocytes were seen in the openings of most vessels. Conclusion. Histological examination of uterine tissues showed the presence of specific changes during the studied time intervals after death, which can be used to introduce criteria to determine the time since death in practice.","PeriodicalId":19107,"journal":{"name":"Morphologia","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79348466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-25DOI: 10.26641/1997-9665.2020.4.29-34
O. Gavrilyuk, S. A. Tomashova, H. L. Stolіar
Background. While examining regional lymph nodes removed with the tumor, the pathomorphologist is often limited to the exclusion of metastases, omitting changes in lymphoid tissue. The aim of the work is to describe a rare variant of Castleman-like lymphadenopathy in the surgical material obtained during the removal of colon cancer with regional lymph nodes. Methods. Pathomorphological examination of removed lymph nodes using histological and immunohistochemical methods (CD23). Results. A 73-year-old patient underwent surgery for rectal cancer. Pathomorphological analysis diagnosed adenocarcinoma of high degree of differentiation (G1) and revealed Castleman-like changes in some of the enlarged regional mesenteric lymph nodes: signs of different stages of development of lymphoid follicles, including their hyperplasia, atresia and fragmentation with preservation of activated CD23+ follicular dendritic cells and reaction of microvasculature, including vascular proliferation in the interfollicular areas with uneven narrowing of the sinuses. The discussion analyzes the probable genesis of changes in lymphoid tissue, which may be a reflection of the early stage of Castleman's disease, complication of radiation therapy performed in the preoperative stage, or a consequence of impaired immune status due to tumor growth, in particular with antigenic overloading of draining lymphoid tissue, effects of cytokines produced by inflammatory infiltrate cells that accumulate directly near the tumor, and the formation of a state of genetic instability, which progresses under the influence of radiation. Conclusion. Despite the rarity of Castleman-like lymphadenopathy, in each case of examination of lymph nodes in the surgical material it is necessary to conduct a thorough analysis of lymphoid tissue, as this may be important both to assess the prognosis and to select adequate therapy.
{"title":"Castleman lymphadenopathy: case review","authors":"O. Gavrilyuk, S. A. Tomashova, H. L. Stolіar","doi":"10.26641/1997-9665.2020.4.29-34","DOIUrl":"https://doi.org/10.26641/1997-9665.2020.4.29-34","url":null,"abstract":"Background. While examining regional lymph nodes removed with the tumor, the pathomorphologist is often limited to the exclusion of metastases, omitting changes in lymphoid tissue. The aim of the work is to describe a rare variant of Castleman-like lymphadenopathy in the surgical material obtained during the removal of colon cancer with regional lymph nodes. Methods. Pathomorphological examination of removed lymph nodes using histological and immunohistochemical methods (CD23). Results. A 73-year-old patient underwent surgery for rectal cancer. Pathomorphological analysis diagnosed adenocarcinoma of high degree of differentiation (G1) and revealed Castleman-like changes in some of the enlarged regional mesenteric lymph nodes: signs of different stages of development of lymphoid follicles, including their hyperplasia, atresia and fragmentation with preservation of activated CD23+ follicular dendritic cells and reaction of microvasculature, including vascular proliferation in the interfollicular areas with uneven narrowing of the sinuses. The discussion analyzes the probable genesis of changes in lymphoid tissue, which may be a reflection of the early stage of Castleman's disease, complication of radiation therapy performed in the preoperative stage, or a consequence of impaired immune status due to tumor growth, in particular with antigenic overloading of draining lymphoid tissue, effects of cytokines produced by inflammatory infiltrate cells that accumulate directly near the tumor, and the formation of a state of genetic instability, which progresses under the influence of radiation. Conclusion. Despite the rarity of Castleman-like lymphadenopathy, in each case of examination of lymph nodes in the surgical material it is necessary to conduct a thorough analysis of lymphoid tissue, as this may be important both to assess the prognosis and to select adequate therapy.","PeriodicalId":19107,"journal":{"name":"Morphologia","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79381110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-25DOI: 10.26641/1997-9665.2020.4.42-48
M. Pankiv
Background. According to the WHO, the diseases of the musculoskeletal system, which cause disability and mortality, rank fourth in the world following cardiovascular disease, cancer and diabetes. Due to the statistics, every fifth person suffers from back pain. Chronic diseases of musculoskeletal system are also one of the most urgent problems in Ukraine, and about 3.5 million people encountered the problem of musculoskeletal system. Objective. The aim of the study was to investigate the impact of opioids on changes of structural components of the intervertebral disc at the end of the fifth and sixth weeks of the experiment. Methods. The materials of the study were 32 sexually mature, outbred male rats, weighing from 125 to 135 g, aged 4.5 months. Animals were injected with nalbuphine intramuscularly daily in the same intervals (at 10-11 am) for 42 days. This aim was achieved by using microscopic techniques of visualization of cellular components of the rat’s intervertebral disc. Histological specimens were prepared according to the common methods using the dye hematoxylin, eosin, azan according to Heidenhain method, PAS reaction according to McManus and Alcian blue according to Steedman. Results. As a result of experimental opioid exposure for 35 and 42 days, we discovered that in 35 days the gelatinous nucleus was reduced and deformed. Notochondral cells were rare. Mostly cell-free zones with a somewhat compacted matrix were localized in the gelatinous nucleus. In 42 days, there were significant structural changes in the gelatinous nucleus, as well as in the structural elements of the fibrous ring and cartilaginous locking plates. The amount of notochondral cells was reduced. The cytoplasm of some notochondral cells was compacted. Conclusion. Pathomorphological changes in the structural components of the intervertebral disc while applying the experimental opioid effect at the end of the 35th and 42nd days will serve as a basis for further study and comparison of the manifestations of opioid chondropathy in the longer periods applying experimental opioid impact.
{"title":"The dynamics of morphological changes of structural components of the intervertebral disc at the end of the thirty-fifth and forty-second days while applying the experimental opioid impact","authors":"M. Pankiv","doi":"10.26641/1997-9665.2020.4.42-48","DOIUrl":"https://doi.org/10.26641/1997-9665.2020.4.42-48","url":null,"abstract":"Background. According to the WHO, the diseases of the musculoskeletal system, which cause disability and mortality, rank fourth in the world following cardiovascular disease, cancer and diabetes. Due to the statistics, every fifth person suffers from back pain. Chronic diseases of musculoskeletal system are also one of the most urgent problems in Ukraine, and about 3.5 million people encountered the problem of musculoskeletal system. Objective. The aim of the study was to investigate the impact of opioids on changes of structural components of the intervertebral disc at the end of the fifth and sixth weeks of the experiment. Methods. The materials of the study were 32 sexually mature, outbred male rats, weighing from 125 to 135 g, aged 4.5 months. Animals were injected with nalbuphine intramuscularly daily in the same intervals (at 10-11 am) for 42 days. This aim was achieved by using microscopic techniques of visualization of cellular components of the rat’s intervertebral disc. Histological specimens were prepared according to the common methods using the dye hematoxylin, eosin, azan according to Heidenhain method, PAS reaction according to McManus and Alcian blue according to Steedman. Results. As a result of experimental opioid exposure for 35 and 42 days, we discovered that in 35 days the gelatinous nucleus was reduced and deformed. Notochondral cells were rare. Mostly cell-free zones with a somewhat compacted matrix were localized in the gelatinous nucleus. In 42 days, there were significant structural changes in the gelatinous nucleus, as well as in the structural elements of the fibrous ring and cartilaginous locking plates. The amount of notochondral cells was reduced. The cytoplasm of some notochondral cells was compacted. Conclusion. Pathomorphological changes in the structural components of the intervertebral disc while applying the experimental opioid effect at the end of the 35th and 42nd days will serve as a basis for further study and comparison of the manifestations of opioid chondropathy in the longer periods applying experimental opioid impact.","PeriodicalId":19107,"journal":{"name":"Morphologia","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84714289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-25DOI: 10.26641/1997-9665.2020.4.122-124
Editorial office Of journal "Morphologia"
Конференція присвячена 150-й річниці від дня народження професора В.П. Карпова – засновника кафедри гістології та першого ректора Дніпропетровської (Катеринославської) медичної академії.4-6 листопада 2020 року на базі ДЗ «ДМА» відбулась Четверта Всеукраїнська науково-практична конференція з міжнародною участю «Теорія та практика сучасної морфології», яка проводилась в онлайн-режимі та була присвячена 150-й річниці від дня народження професора В.П. Карпова – засновника кафедри гістології та першого ректора Дніпропетровської (Катеринославської) медичної академії. Перед початком конференції голова оргкомітету Ігор Твердохліб повідомив, що в її засіданнях беруть участь учасники з понад 150 кафедр і підрозділів із різних навчальних, наукових і клінічних установ України та інших країн. Відкриття розпочалось з промови голови конференції, ректора академії, в якій Тетяна Перцева наголосила на інтеграційному векторі конференції, зазначила важливість проведення подібних заходів для формування як фундаментальних, так і клінічних досліджень, а також окреслила сучасні світові тенденції розвитку морфології та основні шляхи його реалізації в нашій академії. З привітальним словом до делегатів конференції звернулись: голова програмного комітету конференції професор Ігор Шпонька; президент Наукового товариства анатомів, гістологів, ембріологів та топографоанатомів України член-кореспондент НАМН України, професор Юрій Чайковський; президент Асоціації патологів України професор Сергій Гичка. На першому пленарному засіданні з доповідями виступили Олександр Луцик (Львів), Сергій Гичка (Київ), Тетяна Христенко (Запоріжжя), Олена Дядик (Київ), Петро Гриценко (Дніпро), Наталія Мар’єнко й Олександр Степаненко (Харків), які висвітлили основні досягнення вітчизняних морфологічних шкіл у співробітництві з вченими інших країн. Особливо цікавими для учасників конференції були круглі столи та майстер-класи, що були проведені Сергієм Луговським (Київ) і Олександром Бондаренком (Дніпро). Матеріали своїх досліджень також представили зарубіжні дослідники – Катерина Федіна (Гродно, Бєларусь), Антон Гапонов (Єкатеринбург, Російська Федерація). Результати клінічних досліджень з використанням морфологічних методів у галузі травматології надала Тетяна Зуб (Дніпро). На завершення першого дня конференції відбулася вільна дискусія щодо медико-біологічних досліджень у сучасному світі та ефективного співробітництва, яку провів Артур Інджикулян (Бостон, США). Наступний день конференції розпочався з майстер-класу та доповіді Олександра Ковальчука (Київ) щодо особливостей використання імерсивних технологій в медичній освіті та практиці, а також Ніни Бондаренко (Дніпро) про застосування трансмісійної електронної мікроскопії в сучасних наукових дослідженнях. Продовженням роботи конференції стали повідомлення зарубіжних колег Катерини Мостової (Єкатеринбург, Російська Федерация) і Марини Шабалєвої (Гомель, Бєларусь), які були пов’язані з розвитком інформаційних і дистанційних технологій викладання
{"title":"ЧЕТВЕРТА ВСЕУКРАЇНСЬКА НАУКОВО-ПРАКТИЧНА КОНФЕРЕНЦІЯ З МІЖНАРОДНОЮ УЧАСТЮ «ТЕОРІЯ ТА ПРАКТИКА СУЧАСНОЇ МОРФОЛОГІЇ» ДНІПРО - 2020","authors":"Editorial office Of journal \"Morphologia\"","doi":"10.26641/1997-9665.2020.4.122-124","DOIUrl":"https://doi.org/10.26641/1997-9665.2020.4.122-124","url":null,"abstract":"Конференція присвячена 150-й річниці від дня народження професора В.П. Карпова – засновника кафедри гістології та першого ректора Дніпропетровської (Катеринославської) медичної академії.4-6 листопада 2020 року на базі ДЗ «ДМА» відбулась Четверта Всеукраїнська науково-практична конференція з міжнародною участю «Теорія та практика сучасної морфології», яка проводилась в онлайн-режимі та була присвячена 150-й річниці від дня народження професора В.П. Карпова – засновника кафедри гістології та першого ректора Дніпропетровської (Катеринославської) медичної академії. Перед початком конференції голова оргкомітету Ігор Твердохліб повідомив, що в її засіданнях беруть участь учасники з понад 150 кафедр і підрозділів із різних навчальних, наукових і клінічних установ України та інших країн. Відкриття розпочалось з промови голови конференції, ректора академії, в якій Тетяна Перцева наголосила на інтеграційному векторі конференції, зазначила важливість проведення подібних заходів для формування як фундаментальних, так і клінічних досліджень, а також окреслила сучасні світові тенденції розвитку морфології та основні шляхи його реалізації в нашій академії. З привітальним словом до делегатів конференції звернулись: голова програмного комітету конференції професор Ігор Шпонька; президент Наукового товариства анатомів, гістологів, ембріологів та топографоанатомів України член-кореспондент НАМН України, професор Юрій Чайковський; президент Асоціації патологів України професор Сергій Гичка. На першому пленарному засіданні з доповідями виступили Олександр Луцик (Львів), Сергій Гичка (Київ), Тетяна Христенко (Запоріжжя), Олена Дядик (Київ), Петро Гриценко (Дніпро), Наталія Мар’єнко й Олександр Степаненко (Харків), які висвітлили основні досягнення вітчизняних морфологічних шкіл у співробітництві з вченими інших країн. Особливо цікавими для учасників конференції були круглі столи та майстер-класи, що були проведені Сергієм Луговським (Київ) і Олександром Бондаренком (Дніпро). Матеріали своїх досліджень також представили зарубіжні дослідники – Катерина Федіна (Гродно, Бєларусь), Антон Гапонов (Єкатеринбург, Російська Федерація). Результати клінічних досліджень з використанням морфологічних методів у галузі травматології надала Тетяна Зуб (Дніпро). На завершення першого дня конференції відбулася вільна дискусія щодо медико-біологічних досліджень у сучасному світі та ефективного співробітництва, яку провів Артур Інджикулян (Бостон, США). Наступний день конференції розпочався з майстер-класу та доповіді Олександра Ковальчука (Київ) щодо особливостей використання імерсивних технологій в медичній освіті та практиці, а також Ніни Бондаренко (Дніпро) про застосування трансмісійної електронної мікроскопії в сучасних наукових дослідженнях. Продовженням роботи конференції стали повідомлення зарубіжних колег Катерини Мостової (Єкатеринбург, Російська Федерация) і Марини Шабалєвої (Гомель, Бєларусь), які були пов’язані з розвитком інформаційних і дистанційних технологій викладання ","PeriodicalId":19107,"journal":{"name":"Morphologia","volume":"232 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73524526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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