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Prenatal and postnatal influences on behavioral development in a mouse model of preconceptional stress 产前和产后对受孕前应激小鼠模型行为发育的影响
IF 5 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-02-03 DOI: 10.1016/j.ynstr.2024.100614
Joseph Scarborough , Monica Iachizzi , Sina M. Schalbetter , Flavia S. Müller , Ulrike Weber-Stadlbauer , Juliet Richetto

Depression during pregnancy is detrimental for the wellbeing of the expectant mother and can exert long-term consequences on the offspring's development and mental health. In this context, both the gestational environment and the postpartum milieu may be negatively affected by the depressive pathology. It is, however, challenging to assess whether the contributions of prenatal and postnatal depression exposure are distinct, interactive, or cumulative, as it is unclear whether antenatal effects are due to direct effects on fetal development or because antenatal symptoms continue postnatally. Preclinical models have sought to answer this question by implementing stressors that induce a depressive-like state in the dams during pregnancy and studying the effects on the offspring. The aim of our present study was to disentangle the contribution of direct stress in utero from possible changes in maternal behavior in a novel model of preconceptional stress based on social isolation rearing (SIR). Using a cross-fostering paradigm in this model, we show that while SIR leads to subtle changes in maternal behavior, the behavioral changes observed in the offspring are driven by a complex interaction between sex, and prenatal and postnatal maternal factors. Indeed, male offspring are more sensitive to the prenatal environment, as demonstrated by behavioral and transcriptional changes driven by their birth mother, while females are likely affected by more complex interactions between the pre and the postpartum milieu, as suggested by the important impact of their surrogate foster mother. Taken together, our findings suggest that male and female offspring have different time-windows and behavioral domains of susceptibility to maternal preconceptional stress, and thus underscore the importance of including both sexes when investigating the mechanisms that mediate the negative consequences of exposure to such stressor.

孕期抑郁症不利于孕妇的健康,并可能对后代的发育和心理健康造成长期影响。在这种情况下,妊娠环境和产后环境都可能受到抑郁病理的负面影响。然而,评估产前和产后抑郁暴露的贡献是不同的、交互的还是累积的,是一项挑战,因为目前还不清楚产前影响是由于对胎儿发育的直接影响,还是因为产前症状在产后仍在继续。临床前模型试图回答这个问题,方法是在母体妊娠期间施加能诱发类似抑郁状态的压力,并研究其对后代的影响。我们本研究的目的是在一种基于社会隔离饲养(SIR)的新型孕前压力模型中,将子宫内的直接压力与母体行为的可能变化区分开来。通过在该模型中使用交叉寄养范式,我们发现虽然社会隔离饲养会导致母体行为的微妙变化,但在后代身上观察到的行为变化是由性别、产前和产后母体因素之间复杂的相互作用所驱动的。事实上,雄性后代对产前环境更敏感,其行为和转录变化由生母驱动,而雌性后代则可能受到产前和产后环境之间更复杂的相互作用的影响,代养母亲的重要影响也说明了这一点。总之,我们的研究结果表明,男性和女性的后代易受孕前母体压力影响的时间窗口和行为领域不同,因此在研究介导暴露于这种压力的负面影响的机制时,将两性都包括在内非常重要。
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引用次数: 0
Genetic disruption of dopamine β-hydroxylase dysregulates innate responses to predator odor in mice 基因干扰多巴胺β-羟化酶会导致小鼠对捕食者气味的先天反应失调
IF 5 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-02-02 DOI: 10.1016/j.ynstr.2024.100612
Joyce Liu , Daniel J. Lustberg , Abigail Galvez, L. Cameron Liles, Katharine E. McCann, David Weinshenker

In rodents, exposure to predator odors such as cat urine acts as a severe stressor that engages innate defensive behaviors critical for survival in the wild. The neurotransmitters norepinephrine (NE) and dopamine (DA) modulate anxiety and predator odor responses, and we have shown previously that dopamine β-hydroxylase knockout (Dbh −/−), which reduces NE and increases DA in mouse noradrenergic neurons, disrupts innate behaviors in response to mild stressors such as novelty. We examined the consequences of Dbh knockout on responses to predator odor (bobcat urine) and compared them to Dbh-competent littermate controls. Over the first 10 min of predator odor exposure, controls exhibited robust defensive burying behavior, whereas Dbh −/− mice showed high levels of grooming. Defensive burying was potently suppressed in controls by drugs that reduce NE transmission, while excessive grooming in Dbh −/− mice was blocked by DA receptor antagonism. In response to a cotton square scented with a novel “neutral” odor (lavender), most control mice shredded the material, built a nest, and fell asleep within 90 min. Dbh −/− mice failed to shred the lavender-scented nestlet, but still fell asleep. In contrast, controls sustained high levels of arousal throughout the predator odor test and did not build nests, while Dbh −/− mice were asleep by the 90-min time point, often in shredded bobcat urine-soaked nesting material. Compared with controls exposed to predator odor, Dbh −/− mice demonstrated decreased c-fos induction in the anterior cingulate cortex, lateral septum, periaqueductal gray, and bed nucleus of the stria terminalis, but increased c-fos in the locus coeruleus and medial amygdala. These data indicate that relative ratios of central NE and DA signaling coordinate the type and valence of responses to predator odor.

对于啮齿类动物来说,暴露于捕食者气味(如猫尿)是一种严重的应激反应,会引发对野外生存至关重要的先天防御行为。神经递质去甲肾上腺素(NE)和多巴胺(DA)调节焦虑和捕食者气味反应,我们之前已经证明,多巴胺β-羟化酶敲除(Dbh -/-)会减少小鼠去甲肾上腺素能神经元中的NE,增加DA,从而破坏对新奇事物等轻度应激源的先天性行为。我们研究了Dbh基因敲除(Dbh -/-)对捕食者气味(山猫尿液)反应的影响,并将其与Dbh功能正常的同窝对照组进行了比较。在暴露于捕食者气味的最初10分钟内,对照组小鼠表现出强烈的防御性掩埋行为,而Dbh -/-小鼠则表现出高水平的梳理行为。降低NE传递的药物可有效抑制对照组的防御性掩埋行为,而Dbh -/-小鼠的过度梳理行为可被DA受体拮抗剂阻断。对于带有新奇 "中性 "气味(薰衣草)的棉花方块,大多数对照组小鼠会在90分钟内将其撕碎、筑巢并入睡。Dbh -/-小鼠未能撕碎薰衣草香味的小窝,但仍然睡着了。相比之下,对照组在整个捕食者气味测试过程中都保持着高水平的唤醒,并且没有筑巢,而Dbh -/-小鼠在90分钟的时间点前就已经睡着了,通常是在山猫尿液浸泡过的碎巢材料中睡着的。与暴露于捕食者气味的对照组相比,Dbh -/-小鼠在前扣带回皮层、外侧隔膜、丘脑周围灰质和纹状体末端床核中的c-fos诱导减少,但在脑室和内侧杏仁核中的c-fos诱导增加。这些数据表明,中枢NE和DA信号的相对比例协调了对捕食者气味的反应类型和情绪。
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引用次数: 0
Frontal cortical volume deficits as enduring evidence of childhood abuse in community adults with AUD and HIV infection comorbidity 额叶皮质体积缺陷是社区成人中合并有 AUD 和 HIV 感染者童年受虐待的持久证据
IF 5 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-01-22 DOI: 10.1016/j.ynstr.2024.100608
Stephanie A. Sassoon , Rosemary Fama , Kilian M. Pohl , Adolf Pfefferbaum , Edith V. Sullivan

Background

Childhood abuse is an underappreciated source of stress, associated with adverse mental and physical health consequences. Childhood abuse has been directly associated with risky behavior thereby increasing the likelihood of alcohol misuse and risk of HIV infection, conditions associated with brain structural and functional deficits. Here, we examined the neural and behavioral correlates of childhood trauma history in alcohol use disorder (AUD), HIV infection (HIV), and their comorbidity (AUD+HIV).

Methods

Occurrence of childhood trauma was evaluated by retrospective interview. Cortical (frontal, temporal, parietal, and occipital), subcortical (hippocampus, amygdala), and regional frontal volumes were derived from structural MRI, adjusted for intracranial volume and age. Test scores of executive functioning, attention/working memory, verbal/visual learning, verbal/visual memory, and motor speed functional domains were standardized on age and education of a laboratory control group.

Results

History of childhood abuse was associated with smaller frontal lobe volumes regardless of diagnosis. For frontal subregional volumes, history of childhood abuse was selectively associated with smaller orbitofrontal and supplementary motor volumes. In participants with a child abuse history, poorer verbal/visual memory performance was associated with smaller orbitofrontal and frontal middle volumes, whereas in those without childhood abuse, poorer verbal/visual memory performance was associated with smaller orbitofrontal, frontal superior, and supplemental motor volumes.

Conclusions

Taken together, these results comport with and extend the findings that childhood abuse is associated with brain and behavioral sequelae in AUD, HIV, and AUD+HIV comorbidity. Further, these findings suggest that sequelae of abuse in childhood may be best conceptualized as a spectrum disorder as significant deficits may be present in those who may not meet criteria for a formal trauma-related diagnosis yet may be suffering enduring stress effects on brain structural and functional health.

背景童年虐待是一种未被充分认识的压力来源,与不良的身心健康后果相关。童年虐待与危险行为直接相关,从而增加了酗酒的可能性和感染艾滋病病毒的风险,这些情况与大脑结构和功能缺陷有关。在此,我们研究了酗酒障碍(AUD)、艾滋病病毒感染(HIV)及其合并症(AUD+HIV)中童年创伤史的神经和行为相关性。皮质(额叶、颞叶、顶叶和枕叶)、皮质下(海马、杏仁核)和区域额叶体积由结构性核磁共振成像得出,并根据颅内体积和年龄进行了调整。执行功能、注意力/工作记忆、言语/视觉学习、言语/视觉记忆和运动速度等功能领域的测试得分根据实验室对照组的年龄和教育程度进行了标准化处理。就额叶亚区体积而言,童年虐待史选择性地与眶额叶和辅助运动区体积较小有关。在有儿童虐待史的参与者中,较差的言语/视觉记忆表现与较小的眶额叶和额叶中部体积有关,而在没有儿童虐待史的参与者中,较差的言语/视觉记忆表现与较小的眶额叶、额叶上部和辅助运动体积有关。此外,这些研究结果表明,童年受虐后遗症最好被概念化为一种谱系障碍,因为那些可能不符合正式创伤相关诊断标准,但可能对大脑结构和功能健康造成持久应激影响的人可能存在明显缺陷。
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引用次数: 0
Imaging a putative marker of brain cortisol regulation in alcohol use disorder 酒精使用障碍中大脑皮质醇调节的假定标记成像
IF 5 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-01-21 DOI: 10.1016/j.ynstr.2024.100609
Terril L. Verplaetse , Ansel T. Hillmer , Shivani Bhatt , Aleksandra Rusowicz , Songye Li , Nabeel Nabulsi , David Matuskey , Yiyun Huang , Sherry A. McKee , Kelly P. Cosgrove

Background

Stress is a potent activator of the hypothalamic-pituitary-adrenal (HPA) axis, initiating the release of glucocorticoid hormones, such as cortisol. Alcohol consumption can lead to HPA axis dysfunction, including altered cortisol levels. Until recently, research has only been able to examine peripheral cortisol associated with alcohol use disorder (AUD) in humans. We used positron emission tomography (PET) brain imaging with the radiotracer [18F]AS2471907 to measure 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1), a cortisol-regenerating enzyme, in people with AUD compared to healthy controls.

Methods

We imaged 9 individuals with moderate to severe AUD (5 men, 4 women; mean age = 38 years) and 12 healthy controls (8 men, 4 women; mean age = 29 years). Participants received 93.5 ± 15.6 MBq of the 11β-HSD1 inhibitor radiotracer [18F]AS2471907 as a bolus injection and were imaged for 150–180 min on the High-Resolution Research Tomograph. 11β-HSD1 availability was quantified by [18F]AS2471907 volume of distribution (VT; mL/cm3). A priori regions of interest included amygdala, anterior cingulate cortex (ACC), hippocampus, ventromedial PFC (vmPFC) and caudate.

Results

Individuals with AUD consumed 52.4 drinks/week with 5.8 drinking days/week. Healthy controls consumed 2.8 drinks/week with 1.3 drinking days/week. Preliminary findings suggest that [18F]AS2471907 VT was higher in amygdala, ACC, hippocampus, vmPFC, and caudate of those with AUD compared to healthy controls (p < 0.05). In AUD, vmPFC [18F]AS2471907 VT was associated with drinks per week (r = 0.81, p = 0.01) and quantity per drinking episode (r = 0.75, p = 0.02).

Conclusions

This is the first in vivo examination of 11β-HSD1 availability in individuals with AUD. Our data suggest higher brain availability of the cortisol-regenerating enzyme 11β-HSD1 in people with AUD (vs. controls), and that higher vmPFC 11β-HSD1 availability is related to greater alcohol consumption. Thus, in addition to the literature suggesting that people with AUD have elevated peripheral cortisol, our findings suggest there may also be heightened central HPA activity. These findings set the foundation for future hypotheses on mechanisms related to HPA axis function in this population.

背景压力是下丘脑-垂体-肾上腺(HPA)轴的强效激活剂,可启动皮质醇等糖皮质激素的释放。饮酒会导致 HPA 轴功能紊乱,包括皮质醇水平的改变。直到最近,研究才得以检测与人类酒精使用障碍(AUD)相关的外周皮质醇。我们使用放射性示踪剂 [18F]AS2471907 进行正电子发射断层扫描(PET)脑成像,测量 AUD 患者与健康对照组的皮质醇再生酶 11β- 羟类固醇脱氢酶 1 型 (11β-HSD1)。参与者接受了 93.5 ± 15.6 MBq 的 11β-HSD1 抑制剂放射性示踪剂 [18F]AS2471907 的栓剂注射,并在高分辨率研究断层成像仪上进行了 150-180 分钟的成像。11β-HSD1的可用性通过[18F]AS2471907分布容积(VT;mL/cm3)进行量化。先验相关区域包括杏仁核、前扣带回皮层(ACC)、海马、腹内侧 PFC(vmPFC)和尾状核。健康对照组每周饮酒 2.8 次,每周饮酒天数为 1.3 天。初步研究结果表明,与健康对照组相比,AUD患者杏仁核、ACC、海马、vmPFC和尾状核的[18F]AS2471907 VT更高(p <0.05)。在 AUD 患者中,vmPFC [18F]AS2471907 VT 与每周饮酒量(r = 0.81,p = 0.01)和每次饮酒量(r = 0.75,p = 0.02)相关。我们的数据表明,在 AUD 患者(与对照组相比)中,大脑中皮质醇再生酶 11β-HSD1 的可用性较高,而且大脑皮质功能区 11β-HSD1 的可用性较高与饮酒量较大有关。因此,除了有文献表明 AUD 患者的外周皮质醇升高外,我们的研究结果还表明他们的中枢 HPA 活动也可能升高。这些发现为今后假设该人群 HPA 轴功能的相关机制奠定了基础。
{"title":"Imaging a putative marker of brain cortisol regulation in alcohol use disorder","authors":"Terril L. Verplaetse ,&nbsp;Ansel T. Hillmer ,&nbsp;Shivani Bhatt ,&nbsp;Aleksandra Rusowicz ,&nbsp;Songye Li ,&nbsp;Nabeel Nabulsi ,&nbsp;David Matuskey ,&nbsp;Yiyun Huang ,&nbsp;Sherry A. McKee ,&nbsp;Kelly P. Cosgrove","doi":"10.1016/j.ynstr.2024.100609","DOIUrl":"10.1016/j.ynstr.2024.100609","url":null,"abstract":"<div><h3>Background</h3><p>Stress is a potent activator of the hypothalamic-pituitary-adrenal (HPA) axis, initiating the release of glucocorticoid hormones, such as cortisol. Alcohol consumption can lead to HPA axis dysfunction, including altered cortisol levels. Until recently, research has only been able to examine peripheral cortisol associated with alcohol use disorder (AUD) in humans. We used positron emission tomography (PET) brain imaging with the radiotracer [<sup>18</sup>F]AS2471907 to measure 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1), a cortisol-regenerating enzyme, in people with AUD compared to healthy controls.</p></div><div><h3>Methods</h3><p>We imaged 9 individuals with moderate to severe AUD (5 men, 4 women; mean age = 38 years) and 12 healthy controls (8 men, 4 women; mean age = 29 years). Participants received 93.5 ± 15.6 MBq of the 11β-HSD1 inhibitor radiotracer [<sup>18</sup>F]AS2471907 as a bolus injection and were imaged for 150–180 min on the High-Resolution Research Tomograph. 11β-HSD1 availability was quantified by [<sup>18</sup>F]AS2471907 volume of distribution (<em>V</em><sub>T</sub>; mL/cm<sup>3</sup>). <em>A priori</em> regions of interest included amygdala, anterior cingulate cortex (ACC), hippocampus, ventromedial PFC (vmPFC) and caudate.</p></div><div><h3>Results</h3><p>Individuals with AUD consumed 52.4 drinks/week with 5.8 drinking days/week. Healthy controls consumed 2.8 drinks/week with 1.3 drinking days/week. Preliminary findings suggest that [<sup>18</sup>F]AS2471907 <em>V</em><sub>T</sub> was higher in amygdala, ACC, hippocampus, vmPFC, and caudate of those with AUD compared to healthy controls (<em>p</em> &lt; 0.05). In AUD, vmPFC [<sup>18</sup>F]AS2471907 <em>V</em><sub>T</sub> was associated with drinks per week (r = 0.81, <em>p</em> = 0.01) and quantity per drinking episode (r = 0.75, <em>p</em> = 0.02).</p></div><div><h3>Conclusions</h3><p>This is the first <em>in vivo</em> examination of 11β-HSD1 availability in individuals with AUD. Our data suggest higher brain availability of the cortisol-regenerating enzyme 11β-HSD1 in people with AUD (vs. controls), and that higher vmPFC 11β-HSD1 availability is related to greater alcohol consumption. Thus, in addition to the literature suggesting that people with AUD have elevated peripheral cortisol, our findings suggest there may also be heightened central HPA activity. These findings set the foundation for future hypotheses on mechanisms related to HPA axis function in this population.</p></div>","PeriodicalId":19125,"journal":{"name":"Neurobiology of Stress","volume":"29 ","pages":"Article 100609"},"PeriodicalIF":5.0,"publicationDate":"2024-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352289524000055/pdfft?md5=2ad0ca0c2b05a36a7d0a86365f2daa6f&pid=1-s2.0-S2352289524000055-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139539676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Early resource scarcity causes cortical astrocyte enlargement and sex-specific changes in the orbitofrontal cortex transcriptome in adult rats 早期资源匮乏导致成年大鼠皮质星形胶质细胞增大和眶额叶皮质转录组的性别特异性变化
IF 5 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-01-15 DOI: 10.1016/j.ynstr.2024.100607
Claire Deckers , Reza Karbalaei , Nylah A. Miles , Eden V. Harder , Emily Witt , Erin P. Harris , Kathryn Reissner , Mathieu E. Wimmer , Debra A. Bangasser

Astrocyte morphology affects function, including the regulation of glutamatergic signaling. This morphology changes dynamically in response to the environment. However, how early life manipulations alter adult cortical astrocyte morphology is underexplored. Our lab uses brief postnatal resource scarcity, the limited bedding and nesting (LBN) manipulation, in rats. We previously found that LBN augments maternal behaviors and promotes later resilience to adult addiction-related behaviors, reducing impulsivity, risky decision-making, and morphine self-administration. These behaviors rely on glutamatergic transmission in the medial orbitofrontal (mOFC) and medial prefrontal (mPFC) cortex. Here we tested whether LBN changed astrocyte morphology in the mOFC and mPFC of adult rats using a novel viral approach that, unlike traditional markers, fully labels astrocytes. Prior exposure to LBN causes an increase in the surface area and volume of astrocytes in the mOFC and mPFC of adult males and females relative to control-raised rats. We next used bulk RNA sequencing of OFC tissue to assess transcriptional changes that could increase astrocyte size in LBN rats. LBN caused mainly sex-specific changes in differentially expressed genes. Pathway analysis revealed that OFC glutamatergic signaling is altered by LBN in males and females, but the gene changes in that pathway differed across sex. This may represent a convergent sex difference where glutamatergic signaling, which affects astrocyte morphology, is altered by LBN via sex-specific mechanisms. Collectively, these studies highlight that astrocytes may be an important cell type that mediates the effect of early resource scarcity on adult brain function.

星形胶质细胞的形态会影响其功能,包括对谷氨酸能信号传导的调控。这种形态会随着环境的变化而发生动态变化。然而,对生命早期的操作如何改变成年皮质星形胶质细胞形态的研究还很欠缺。我们的实验室利用大鼠出生后短暂的资源匮乏,即有限的垫料和筑巢(LBN)操作。我们之前发现,LBN 可促进成年后对成瘾相关行为的恢复能力,减少冲动、风险决策和吗啡自我给药。这些行为依赖于内侧眶额叶(mOFC)和内侧前额叶(mPFC)皮层的谷氨酸能传导。在这里,我们使用一种新颖的病毒方法测试了 LBN 是否会改变成年大鼠 mOFC 和 mPFC 中星形胶质细胞的形态。与对照组大鼠相比,事先暴露于 LBN 会导致成年雄性大鼠和雌性大鼠 mOFC 和 mPFC 中星形胶质细胞的表面积和体积增加。接下来,我们使用 OFC 组织的大量 RNA 测序来评估 LBN 大鼠中可能增加星形胶质细胞体积的转录变化。LBN 主要导致了不同表达基因的性别特异性变化。通路分析表明,LBN 会改变雄性和雌性 OFC 谷氨酸能信号传导,但该通路的基因变化在不同性别间存在差异。这可能代表了一种趋同的性别差异,即影响星形胶质细胞形态的谷氨酸能信号通过性别特异性机制被 LBN 改变。总之,这些研究强调星形胶质细胞可能是介导早期资源匮乏对成年大脑功能影响的重要细胞类型。
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引用次数: 0
Fear conditioning and extinction distinctively alter bidirectional synaptic plasticity within the amygdala of an animal model of post-traumatic stress disorder 恐惧调节和消退会明显改变创伤后应激障碍幼年动物模型杏仁核内的双向突触可塑性
IF 5 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-01-12 DOI: 10.1016/j.ynstr.2024.100606
Kwanghoon Park, Hoyong Park, ChiHye Chung

Synaptic plasticity in the amygdala plays an essential role in the formation and inhibition of fear memory; however, this plasticity has mainly been studied in the lateral amygdala, making it largely uninvestigated in other subnuclei. Here, we investigated long-term potentiation (LTP) and long-term depression (LTD) in the basolateral amygdala (BLA) to the medial division of the central amygdala (CEm) synapses of juvenile C57BL/6N (B6) and 129S1/SvImJ (S1) mice. We found that in naïve B6 and S1 mice, LTP was not induced at the BLA to CEm synapses, whereas fear conditioning lowered the threshold for LTP induction in these synapses of both B6 and S1 mice. Interestingly, fear extinction disrupted the induction of LTP at the BLA to CEm synapses of B6 mice, whereas LTP was left intact in S1 mice. Both low-frequency stimulation (LFS) and modest LFS (mLFS) induced LTD in naïve B6 and S1 mice, suggesting that the BLA to CEm synapses express bidirectional plasticity. Fear conditioning disrupted both types of LTD induction selectively in S1 mice and LFS-LTD, presumably NMDAR-dependent LTD was partially recovered by fear extinction. However, mLFS-LTD which has been known to be endocannabinoid receptor 1 (CB1R)-dependent was not induced after fear extinction in both mouse strains. Our observations suggest that fear conditioning enhances LTP while fear extinction diminishes LTP at the BLA to the CEm synapses of B6 mice with successful extinction. Considering that S1 mice showed strong fear conditioning and impaired extinction, strong fear conditioning in the S1 strain may be related to disrupted LTD, and impaired extinction may be due to constant LTP and weak LFS-LTD at the BLA to CEm synapses. Our study contributes to the further understanding of the dynamics of synaptic potentiation and depression between the subnuclei of the amygdala in juvenile mice after fear conditioning and extinction.

杏仁核中的突触可塑性在恐惧记忆的形成和抑制过程中起着至关重要的作用;然而,这种可塑性主要是在杏仁核外侧进行研究的,因此在其他亚核中基本上没有研究。在这里,我们研究了幼年 C57BL/6N (B6) 和 129S1/SvImJ (S1) 小鼠杏仁核基底外侧(BLA)到杏仁核中央内侧分部(CEm)突触的长期电位(LTP)和长期抑制(LTD)。我们发现,在天真的B6和S1小鼠中,BLA到CEm突触不会诱导LTP,而恐惧条件反射会降低B6和S1小鼠这些突触的LTP诱导阈值。有趣的是,恐惧消退会破坏B6小鼠BLA至CEm突触的LTP诱导,而S1小鼠的LTP则保持不变。低频刺激(LFS)和适度LFS(mLFS)都能在天真的B6和S1小鼠中诱导LTD,这表明BLA至CEm突触具有双向可塑性。在 S1 小鼠中,恐惧条件选择性地破坏了这两种类型的 LTD 诱导,而 LFS-LTD(推测为 NMDAR 依赖性 LTD)在恐惧消失后得到了部分恢复。然而,已知依赖于内源性大麻素受体 1(CB1R)的 mLFS-LTD 在两种小鼠品系的恐惧消退后都没有被诱导。我们的观察结果表明,恐惧条件反射会增强 LTP,而恐惧消退会降低成功消退的 B6 小鼠 BLA 至 CEm 突触的 LTP。考虑到S1小鼠表现出强烈的恐惧条件反射和消退障碍,S1品系的强烈恐惧条件反射可能与LTTP的破坏有关,而消退障碍可能是由于BLA到CEm突触的LTP不变和LFS-LTD减弱所致。我们的研究有助于进一步了解幼年小鼠在恐惧条件反射和消退后杏仁核亚核之间突触电位增强和抑制的动态变化。
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引用次数: 0
A reverse translational study of PPAR-α agonist efficacy in human and rodent models relevant to alcohol use disorder 关于 PPAR-α 激动剂在与酒精使用障碍有关的人类和啮齿动物模型中疗效的逆向转化研究
IF 5 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-01-05 DOI: 10.1016/j.ynstr.2023.100604
Barbara J. Mason , David Estey , Amanda Roberts , Giordano de Guglielmo , Olivier George , John Light , Mike Stoolmiller , Susan Quello , Michael Skinner , Farhad Shadan , Adnan Begovic , Mark C. Kyle , R. Adron Harris

Alcohol Use Disorder (AUD) is a chronic relapsing disorder affecting an estimated 283 million individuals worldwide, with substantial health and economic consequences. Peroxisome proliferator-activated receptors (PPARs), particularly PPAR-α and PPAR-γ, have shown promise in preclinical studies as potential therapeutic targets for AUD. In this human laboratory study, we aimed to translate preclinical findings on the PPAR-α agonist fenofibrate to a human population with current AUD. We hypothesized that, relative to placebo, fenofibrate at the highest FDA-approved dose of 145 mg/d would attenuate responsiveness to in vivo alcohol cues in the lab and reduce drinking under natural conditions. However, the results did not show significant differences in craving and alcohol consumption between the fenofibrate and placebo groups. Reverse translational studies in rodent models confirmed the lack of fenofibrate effect at human-equivalent doses. These findings suggest that inadequate translation of drug dose from rodents to humans may account for the lack of fenofibrate effects on alcohol craving and consumption in humans with AUD. The results highlight the need for new brain-penetrant PPAR-α agonists to adequately test the therapeutic potential of PPAR-α agonists for AUD, and the importance of reverse translational approaches and selection of human-equivalent doses in drug development.

酒精使用障碍(AUD)是一种慢性复发性疾病,影响着全球约 2.83 亿人,对健康和经济造成严重后果。临床前研究显示,过氧化物酶体增殖激活受体(PPAR),尤其是 PPAR-α 和 PPAR-γ,有望成为 AUD 的潜在治疗靶点。在这项人体实验室研究中,我们的目的是将 PPAR-α 激动剂非诺贝特的临床前研究结果应用于目前患有 AUD 的人群。我们假设,相对于安慰剂,美国食品药品管理局批准的最高剂量(145 毫克/天)的非诺贝特将在实验室中减弱对体内酒精线索的反应,并在自然条件下减少饮酒。然而,研究结果并未显示非诺贝特组和安慰剂组在渴求和饮酒量方面存在显著差异。在啮齿类动物模型中进行的反向转化研究证实,非诺贝特在人体同等剂量下缺乏效果。这些研究结果表明,从啮齿动物到人类的药物剂量转化不足可能是非诺贝特对AUD患者的酒精渴求和饮酒缺乏影响的原因。这些结果突显了需要新的脑穿透性PPAR-α激动剂来充分测试PPAR-α激动剂对AUD的治疗潜力,以及逆向转化方法和选择人体等效剂量在药物开发中的重要性。
{"title":"A reverse translational study of PPAR-α agonist efficacy in human and rodent models relevant to alcohol use disorder","authors":"Barbara J. Mason ,&nbsp;David Estey ,&nbsp;Amanda Roberts ,&nbsp;Giordano de Guglielmo ,&nbsp;Olivier George ,&nbsp;John Light ,&nbsp;Mike Stoolmiller ,&nbsp;Susan Quello ,&nbsp;Michael Skinner ,&nbsp;Farhad Shadan ,&nbsp;Adnan Begovic ,&nbsp;Mark C. Kyle ,&nbsp;R. Adron Harris","doi":"10.1016/j.ynstr.2023.100604","DOIUrl":"10.1016/j.ynstr.2023.100604","url":null,"abstract":"<div><p>Alcohol Use Disorder (AUD) is a chronic relapsing disorder affecting an estimated 283 million individuals worldwide, with substantial health and economic consequences. Peroxisome proliferator-activated receptors (PPARs), particularly PPAR-α and PPAR-γ, have shown promise in preclinical studies as potential therapeutic targets for AUD. In this human laboratory study, we aimed to translate preclinical findings on the PPAR-α agonist fenofibrate to a human population with current AUD. We hypothesized that, relative to placebo, fenofibrate at the highest FDA-approved dose of 145 mg/d would attenuate responsiveness to <em>in vivo</em> alcohol cues in the lab and reduce drinking under natural conditions. However, the results did not show significant differences in craving and alcohol consumption between the fenofibrate and placebo groups. Reverse translational studies in rodent models confirmed the lack of fenofibrate effect at human-equivalent doses. These findings suggest that inadequate translation of drug dose from rodents to humans may account for the lack of fenofibrate effects on alcohol craving and consumption in humans with AUD. The results highlight the need for new brain-penetrant PPAR-α agonists to adequately test the therapeutic potential of PPAR-α agonists for AUD, and the importance of reverse translational approaches and selection of human-equivalent doses in drug development.</p></div>","PeriodicalId":19125,"journal":{"name":"Neurobiology of Stress","volume":"29 ","pages":"Article 100604"},"PeriodicalIF":5.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352289523000929/pdfft?md5=93cc59a2d8f4ecbc4279f315ca745ee6&pid=1-s2.0-S2352289523000929-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139105554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Alcohol and stress exposure across the lifespan are key risk factors for Alzheimer's Disease and cognitive decline 在人的一生中,酒精和压力是导致阿尔茨海默病和认知能力下降的关键风险因素
IF 5 2区 医学 Q1 NEUROSCIENCES Pub Date : 2024-01-04 DOI: 10.1016/j.ynstr.2024.100605
Laurel R. Seemiller , Julio Flores-Cuadra , Keith R. Griffith , Grace C. Smith , Nicole A. Crowley

Alzheimer's Disease and related dementias (ADRD) are an increasing threat to global health initiatives. Efforts to prevent the development of ADRD require understanding behaviors that increase and decrease risk of neurodegeneration and cognitive decline, in addition to uncovering the underlying biological mechanisms behind these effects. Stress exposure and alcohol consumption have both been associated with increased risk for ADRD in human populations. However, our ability to understand causal mechanisms of ADRD requires substantial preclinical research. In this review, we summarize existing human and animal research investigating the connections between lifetime stress and alcohol exposures and ADRD.

阿尔茨海默病和相关痴呆症(ADRD)对全球健康计划的威胁与日俱增。要预防阿尔茨海默病和相关痴呆症的发生,除了要了解增加和减少神经变性和认知能力下降风险的行为外,还需要揭示这些影响背后的生物机制。压力暴露和饮酒都与人类 ADRD 风险的增加有关。然而,我们要了解 ADRD 的因果机制,还需要大量的临床前研究。在本综述中,我们总结了现有的人类和动物研究,这些研究调查了终生压力暴露和酒精暴露与 ADRD 之间的联系。
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引用次数: 0
Inter- and transgenerational heritability of preconception chronic stress or alcohol exposure: Translational outcomes in brain and behavior 孕前慢性压力或酒精暴露的代际和跨代遗传性:大脑和行为的转化结果
IF 5 2区 医学 Q1 NEUROSCIENCES Pub Date : 2023-12-25 DOI: 10.1016/j.ynstr.2023.100603
Rachel C. Rice , Daniela V. Gil , Annalisa M. Baratta , Remy R. Frawley , Shirley Y. Hill , Sean P. Farris , Gregg E. Homanics

Chronic stress and alcohol (ethanol) use are highly interrelated and can change an individual’s behavior through molecular adaptations that do not change the DNA sequence, but instead change gene expression. A recent wealth of research has found that these nongenomic changes can be transmitted across generations, which could partially account for the “missing heritability” observed in genome-wide association studies of alcohol use disorder and other stress-related neuropsychiatric disorders. In this review, we summarize the molecular and behavioral outcomes of nongenomic inheritance of chronic stress and ethanol exposure and the germline mechanisms that could give rise to this heritability. In doing so, we outline the need for further research to: (1) Investigate individual germline mechanisms of paternal, maternal, and biparental nongenomic chronic stress- and ethanol-related inheritance; (2) Synthesize and dissect cross-generational chronic stress and ethanol exposure; (3) Determine cross-generational molecular outcomes of preconception ethanol exposure that contribute to alcohol-related disease risk, using cancer as an example. A detailed understanding of the cross-generational nongenomic effects of stress and/or ethanol will yield novel insight into the impact of ancestral perturbations on disease risk across generations and uncover actionable targets to improve human health.

慢性压力和酒精(乙醇)的使用高度相关,可通过分子适应性改变个体的行为,这些适应性并不改变 DNA 序列,而是改变基因表达。最近的大量研究发现,这些非基因组变化可以跨代传递,这可能部分解释了在酒精使用障碍和其他压力相关神经精神疾病的全基因组关联研究中观察到的 "缺失遗传性"。在这篇综述中,我们总结了慢性压力和乙醇暴露非基因组遗传的分子和行为结果,以及可能导致这种遗传性的种系机制。在此过程中,我们概述了进一步研究的必要性,以便(1) 调查父系、母系和双亲非基因组慢性压力和乙醇相关遗传的种系机制;(2) 综合和剖析跨代慢性压力和乙醇暴露;(3) 以癌症为例,确定孕前乙醇暴露导致酒精相关疾病风险的跨代分子结果。详细了解压力和/或乙醇的跨代非基因组效应将有助于深入了解祖先的干扰对跨代疾病风险的影响,并发现改善人类健康的可行目标。
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引用次数: 0
An epigenetic mechanism of social isolation stress in adolescent female mice 青春期雌性小鼠社会隔离压力的表观遗传机制
IF 5 2区 医学 Q1 NEUROSCIENCES Pub Date : 2023-12-18 DOI: 10.1016/j.ynstr.2023.100601
Pei Li, Zhen Yan

Social isolation during adolescence can increase the risk of mental disorders. Epigenetic changes induced by chronic social isolation may serve as a mechanism underlying emotional disturbances. To test this, we exposed female mice to a post-weaning 6-week social isolation (SI) stress. We found the significantly increased methylation of histone H3 at lysine 9 (H3K9), a histone mark linked to gene repression, as well as the increased H3K9 methyltransferases SUV39H1 and SETDB1, in prefrontal cortex (PFC) of SI females. To find out potential downstream genes affected by this epigenetic alteration, we examined genes linked to neuronal and synaptic functions. Activity-dependent genes, including Arc, c-Fos and Npas4, were significantly reduced in PFC of SI females, correlated with the increased H3K9me2 occupancy around Arc enhancer. Treatment of SI females with UNC0642, a selective inhibitor of H3K9 methylation, significantly attenuated the anxiety-like behavior and elevated Arc expression. These results have revealed an epigenetic mechanism and intervention avenue for anxiety induced by chronic social isolation.

青少年时期的社会隔离会增加患精神疾病的风险。长期社会隔离所诱导的表观遗传变化可能是情绪障碍的一种潜在机制。为了验证这一点,我们将雌性小鼠置于断奶后 6 周的社会隔离(SI)应激中。我们发现,在社会隔离雌性小鼠的前额叶皮层(PFC)中,组蛋白 H3 在赖氨酸 9(H3K9)处的甲基化明显增加,而 H3K9 甲基转移酶 SUV39H1 和 SETDB1 的含量也有所增加。为了找出受这种表观遗传学改变影响的潜在下游基因,我们研究了与神经元和突触功能相关的基因。活动依赖基因,包括 Arc、c-Fos 和 Npas4,在 SI 雌性的 PFC 中显著减少,这与 Arc 增强子周围 H3K9me2 占有率的增加有关。用 H3K9 甲基化的选择性抑制剂 UNC0642 治疗 SI 雌鼠,可显著减轻焦虑样行为和 Arc 表达的升高。这些结果揭示了一种表观遗传学机制和干预长期社会隔离诱发焦虑的途径。
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引用次数: 0
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Neurobiology of Stress
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