Neuroscience最新文献_第2页

Acting with awareness is positively correlated with dorsal anterior cingulate cortex glutamate concentration but both are impaired in Internet gaming disorder 有意识地行动与背侧前扣带回皮层谷氨酸浓度呈正相关，但两者在网络游戏障碍中都会受损。

IF 2.9 3区医学 Q2 NEUROSCIENCES

Neuroscience

Pub Date : 2024-11-24 DOI: 10.1016/j.neuroscience.2024.11.054

Tiantian Hong , Hui Zhou , Wan Xi , Xiumei Li , Yusang Du , Jiaxin Liu , Fengji Geng , Yuzheng Hu

Internet gaming disorder (IGD) is increasingly recognized as a public concern for its adverse impacts on cognition and mental health. In IGD, the transition from goal-directed actions to habitual and eventually compulsive behaviors is accompanied by altered neural response within the dorsal anterior cingulate cortex (dACC), a critical region involved in conscious actions. However, the neurochemical profile of the dACC in IGD and its relationship with behavioral awareness remain poorly understood. In this study, ¹H-magnetic resonance spectroscopy was employed to quantify dACC glutamate concentration and examine its association with the capacity for ‘acting with awareness’ among 21 participants with IGD and 19 recreational game users. Results indicated that dACC glutamate levels and behavioral awareness were significantly lower in the IGD group compared to recreational game users. Moreover, a significant positive correlation between awareness and dACC glutamate concentration emerged in the recreational game users’ group, a relationship attenuated in those with IGD. In an independent cohort of 107 participants, the positive association between awareness and dACC glutamate concentration was replicated. These findings suggest that reduced dACC glutamate in IGD may underlie diminished awareness of maladaptive habitual behaviors. Enhancing dACC neural excitability through neuromodulation or mindfulness training could represent a potential intervention to restore behavioral awareness.

网络游戏障碍（IGD）因其对认知和心理健康的不良影响而日益受到公众关注。在 IGD 中，从目标定向行为到习惯性行为以及最终的强迫性行为的转变，伴随着背侧前扣带回皮层（dACC）神经反应的改变，而 dACC 是参与有意识行为的关键区域。然而，人们对 IGD 中 dACC 的神经化学特征及其与行为意识的关系仍然知之甚少。在这项研究中，21 名 IGD 患者和 19 名休闲游戏使用者采用 1H-磁共振光谱法量化了 dACC 谷氨酸的浓度，并研究了其与 "有意识行动 "能力的关系。结果表明，与休闲游戏使用者相比，IGD 组的 dACC 谷氨酸水平和行为意识明显较低。此外，在休闲游戏用户组中，意识与 dACC 谷氨酸浓度之间存在明显的正相关关系，而在 IGD 患者中，这种关系有所减弱。在一个由 107 名参与者组成的独立队列中，意识与 dACC 谷氨酸浓度之间的正相关关系得到了验证。这些研究结果表明，IGD 患者的 dACC 谷氨酸减少可能是对不良习惯行为的意识减弱的原因。通过神经调节或正念训练来提高dACC神经兴奋性可能是恢复行为意识的一种潜在干预措施。

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引用次数: 0

Diversity Matters. 多样性至关重要。

IF 2.9 3区医学 Q2 NEUROSCIENCES

Neuroscience

Pub Date : 2024-11-23 DOI: 10.1016/j.neuroscience.2024.11.057

Francesca Cirulli, Sarah J Spencer, Chen Zhang

引用次数: 0

Neural correlates of empathy in donation decisions: Insights from EEG and machine learning 捐赠决策中移情的神经相关性：脑电图和机器学习的启示

IF 2.9 3区医学 Q2 NEUROSCIENCES

Neuroscience

Pub Date : 2024-11-23 DOI: 10.1016/j.neuroscience.2024.11.044

Wenhao Mao , Xuejie Shen , Xiaoxu Bai , Ailian Wang

Empathy is central to individual and societal well-being. Numerous studies have examined how trait of empathy affects prosocial behavior. However, little studies explored the psychological and neural mechanisms by which different dimensions of trait empathy influence prosocial behavior. Addressing this knowledge gap is important to understand empathy-driven prosocial behavior. We employed an EEG experiment combined with interpretable machine learning methods to probe these questions. We found that empathic concern (EC) played the most pivotal role in donation decision. Behaviorally, EC negatively moderates the effect of perceived closeness and deservedness of charity projects on the willingness to donate. The machine learning results indicate that EC significantly predicts late positive potential (LPP) and beta-band activity during donation information processing. Further regression analysis results indicate that EC, rather than other dimensions of trait empathy, can positively predict LPP amplitude and negatively predict beta-band activity. These results indicated that participants with higher EC scores may experience heightened emotional arousal and the vicarious experience of others’ emotions while processing donation information. Our work adds weight to understanding the relationship between trait empathy and prosocial behavior and provides electrophysiological evidence.

同理心对个人和社会福祉至关重要。许多研究都探讨了共情特质如何影响亲社会行为。然而，很少有研究探讨特质移情的不同维度影响亲社会行为的心理和神经机制。填补这一知识空白对于理解移情驱动的亲社会行为非常重要。我们采用脑电图实验结合可解释的机器学习方法来探究这些问题。我们发现，移情关注（EC）在捐赠决定中起着最关键的作用。在行为上，EC 负向调节了感知到的慈善项目的亲密性和应得性对捐赠意愿的影响。机器学习结果表明，EC 能显著预测捐赠信息处理过程中的晚期正电位（LPP）和β波段活动。进一步的回归分析结果表明，EC 比特质移情的其他维度更能正向预测 LPP 的振幅，而负向预测 beta 波段的活动。这些结果表明，EC 分数较高的参与者在处理捐赠信息时可能会体验到更高的情绪唤醒和他人情绪的替代体验。我们的研究为理解特质移情与亲社会行为之间的关系增添了砝码，并提供了电生理学证据。

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引用次数: 0

Early changes in intestinal lymphoid and myeloid populations in experimental autoimmune encephalomyelitis 实验性自身免疫性脑脊髓炎肠道淋巴细胞和髓细胞群的早期变化

IF 2.9 3区医学 Q2 NEUROSCIENCES

Neuroscience

Pub Date : 2024-11-22 DOI: 10.1016/j.neuroscience.2024.11.051

Erwan Baudron , Fabian Docagne , Denis Vivien , Isabelle Bardou

Intestinal immunity is associated with several autoimmune diseases, such as systemic lupus erythematosus, rheumatoid arthritis, and type 1 diabetes. Recent evidence also suggests its implication in the pathogenesis of autoimmune diseases affecting the central nervous system, such as multiple sclerosis (MS). However, there is ongoing debate regarding which part of the intestinal tract contributes to the development of MS. Therefore, our study aimed to explore the early changes in lymphoid and myeloid immune cells populations in experimental autoimmune encephalomyelitis (EAE), an animal model of MS. We also sought to determine the roles of the colon and/or small intestine in the pathogenesis of EAE.

By using flow cytometry, we revealed a transient increase in T and B lymphocytes in the ileal lamina propria of EAE mice just before the onset of motor symptoms. Additionally, we highlighted an increase in dendritic cells and monocytes/macrophages in the colonic lamina propria of EAE animals during the presymptomatic phase.

Altogether, our findings indicate that both small intestine and colon are involved in the pathogenesis of EAE, despite engaging distinct immunological processes. This study provides new insights for understanding the roles of intestinal lymphoid and myeloid immune cells on the pathogenesis of MS and other autoimmune diseases.

肠道免疫与多种自身免疫性疾病有关，如系统性红斑狼疮、类风湿性关节炎和 1 型糖尿病。最近的证据还表明，它与影响中枢神经系统的自身免疫性疾病（如多发性硬化症）的发病机制有关。然而，关于肠道的哪个部分会导致多发性硬化症的发生，目前仍存在争议。因此，我们的研究旨在探索实验性自身免疫性脑脊髓炎（EAE）这种多发性硬化症动物模型中淋巴和骨髓免疫细胞群的早期变化。我们还试图确定结肠和/或小肠在 EAE 发病机制中的作用。通过使用流式细胞术，我们发现就在运动症状出现之前，EAE 小鼠回肠固有层中的 T 淋巴细胞和 B 淋巴细胞出现了短暂的增加。此外，我们还发现 EAE 动物的结肠固有膜中树突状细胞和单核细胞/巨噬细胞在症状前阶段有所增加。总之，我们的研究结果表明，尽管小肠和结肠参与了不同的免疫过程，但它们都参与了 EAE 的发病机制。这项研究为了解肠道淋巴和髓系免疫细胞在多发性硬化症和其他自身免疫性疾病发病机制中的作用提供了新的视角。

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引用次数: 0

Decreases in metabolic ATP open KATP channels and reduce firing in an auditory brainstem neuron: A dynamic mechanism of firing control during intense activity 代谢 ATP 的减少打开了 KATP 通道并降低了听觉脑干神经元的发射：强烈活动期间发射控制的动态机制。

IF 2.9 3区医学 Q2 NEUROSCIENCES

Neuroscience

Pub Date : 2024-11-21 DOI: 10.1016/j.neuroscience.2024.11.052

Daniela Vanessa F. de Siqueira, Natalia Boaretto, Ricardo Maurício Leão

Cartwheel (CW) neurons are glycinergic interneurons in the dorsal cochlear nucleus (DCN) that exhibit spontaneous firing, resulting in potent tonic inhibition of fusiform neurons. CW neurons expressing open ATP-sensitive potassium (K_ATP) channels do not fire spontaneously, and activation of K_ATP channels halts spontaneous firing in these neurons. However, the conditions that regulate K_ATP channel opening in CW neurons remain unknown. Here, we tested the hypothesis that fluctuations in metabolic ATP levels modulate K_ATP channels in CW neurons. Using whole-cell patch-clamp recordings in CW neurons from young rat brain slices (p17-22) with an ATP-free internal solution, we observed that the mitochondrial uncoupler CCCP hyperpolarized the membrane potential, reduced spontaneous firing, and generated an outward current, which was inhibited by the K_ATP channel antagonist tolbutamide. Additionally, a glucose-free external solution quickly activated K_ATP channels and ceased spontaneous firing. We hypothesized that intense membrane ion ATPase activity during strong depolarization would deplete intracellular ATP, leading to K_ATP channel opening. Consistent with this, depolarizing CW neurons with a 250 pA DC did not increase spontaneous firing because the depolarization activated K_ATP channels; however, the same depolarization after tolbutamide administration increased firing, suggesting that ATP depletion triggered K_ATP channel opening to limit action potential firing. These results indicate that K_ATP channels in the DCN provide dynamic control over action potential firing, preventing excessive excitation during high-firing activity.

车轮（CW）神经元是背侧耳蜗核（DCN）中的甘氨酸能中间神经元，它表现出自发发射，从而对纺锤形神经元产生强直性抑制。表达开放式 ATP 敏感钾（KATP）通道的 CW 神经元不会自发发射，激活 KATP 通道会阻止这些神经元的自发发射。然而，调控 CW 神经元中 KATP 通道开放的条件仍然未知。在这里，我们测试了代谢 ATP 水平波动调节 CW 神经元 KATP 通道的假设。通过在无 ATP 的内部溶液中对年轻大鼠脑片（p17-22）的 CW 神经元进行全细胞膜片钳记录，我们观察到线粒体解偶联剂 CCCP 可使膜电位超极化，降低自发发射并产生外向电流，而 KATP 通道拮抗剂甲苯磺丁酰胺可抑制外向电流。此外，无葡萄糖的外部溶液可迅速激活 KATP 通道并停止自发发射。我们假设，在强去极化过程中，膜离子 ATP 酶的强烈活动会耗尽细胞内 ATP，从而导致 KATP 通道开放。与此相一致的是，用 250 pA 直流电对 CW 神经元进行去极化并不会增加自发发射，因为去极化激活了 KATP 通道；然而，在服用甲苯磺丁脲后进行同样的去极化却增加了发射，这表明 ATP 耗竭触发了 KATP 通道开放以限制动作电位发射。这些结果表明，DCN 中的 KATP 通道可对动作电位发射进行动态控制，防止在高发射活动期间过度兴奋。

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引用次数: 0

Impacts of maternal separation stress on ethanol intake and endocannabinoid system in adolescent mice. 母体分离应激对青春期小鼠乙醇摄入量和内源性大麻素系统的影响

IF 2.9 3区医学 Q2 NEUROSCIENCES

Neuroscience

Pub Date : 2024-11-21 DOI: 10.1016/j.neuroscience.2024.11.037

C A Favoretto, N B Bertagna, A Anjos-Santos, C M Loss, B T Rodolpho, T Righi, F R Bezerra, P C Bianchi, F C Cruz

Clinical and preclinical studies suggest that early-life stress can increase the risk of developing ethanol use disorder later in life. Although the endocannabinoid (eCB) system plays a role in stress-related behaviors and ethanol consumption, it remains unclear whether the eCB system is affected in response to a combination of both factors. By using male and female adolescent C57BL/6J mice subjected to a maternal separation (MS) stress paradigm from postnatal day (PND) 1 to 14, we explored (1) the consequences of early-life stress experiences on ethanol consumption in adolescent mice and (2) how these events affect the eCB system and neuronal activation in brain regions associated with the reward system. In Experiment 1, we found that MS increased involuntary ethanol consumption specifically during the first exposure to the drug (during a 24 h-long trial on PND 28) and decreased the active/inactive nose poke ratio (discrimination index) specifically when mice were subjected to 1 h-sessions (PND 82-86) in an operant ethanol self-administration paradigm. In Experiment 2, during a two-bottle free choice paradigm, we found that MS increased mice preference for high ethanol concentrations (15 % and 20 %) but not lower ethanol concentrations (5 % and 10 %). Except for Mgll gene expression in the dorsal striatum (DS) in Experiment 2, no statistically significant effects of MS were observed regarding neuronal activation on the prefrontal cortex, DS, globus pallidus, and substantia nigra following a binge operant ethanol self-administration session (Experiment 1) or the eCB system molecules (Cnr1 and Faah gene expression) in the DS (Experiment 2).

临床和临床前研究表明，早期生活压力会增加日后患上乙醇使用障碍的风险。尽管内源性大麻素（eCB）系统在与压力相关的行为和乙醇消费中发挥作用，但目前仍不清楚eCB系统是否会同时受到这两种因素的影响。通过使用雄性和雌性 C57BL/6J 青春期小鼠，从出生后第 1 天到第 14 天对其进行母体分离（MS）应激范式，我们探讨了：（1）早期生活应激经历对青春期小鼠乙醇消耗的影响；（2）这些事件如何影响 eCB 系统以及与奖赏系统相关的脑区的神经元激活。在实验1中，我们发现当小鼠在操作性乙醇自我给药范式中接受1小时的治疗（PND 82-86）时，多发性硬化症会增加小鼠在第一次接触乙醇时（PND 28时长达24小时的试验期间）的非自愿乙醇消耗量，并降低小鼠主动/非主动捅鼻子比率（辨别指数）。在实验2中，我们发现在双瓶自由选择范式中，MS增加了小鼠对高浓度乙醇（15%和20%）的偏好，但没有增加小鼠对低浓度乙醇（5%和10%）的偏好。除了实验 2 中背侧纹状体（DS）中的 Mgll 基因表达外，MS 对狂饮操作性乙醇自我给药后前额叶皮层、DS、苍白球和黑质的神经元激活（实验 1）或 DS 中的 eCB 系统分子（Cnr1 和 Faah 基因表达）（实验 2）均无统计学意义上的显著影响。

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引用次数: 0

Microglial upregulation of CD109 expression in spinal cord of amyotrophic lateral sclerosis mouse model and its role in modulating inflammation and TGFβ/SMAD pathway 肌萎缩性脊髓侧索硬化症小鼠脊髓小胶质细胞CD109表达上调及其在调节炎症和TGFβ/SMAD通路中的作用

IF 2.9 3区医学 Q2 NEUROSCIENCES

Neuroscience

Pub Date : 2024-11-21 DOI: 10.1016/j.neuroscience.2024.11.053

Zhongzhong Li , Yingzhen Zhang , Dongxiao Li , Xinyan Du , Lin Chen , Yansu Guo

CD109 is a multifunctional coreceptor, whose function has been widely studied in tumor progression and metastasis. One of the reported primary roles of CD109 involves down-regulating TGFβ signaling. However, the role of CD109 in central nervous system, especially neurodegenerative disease, is barely known. Here, we examined the expression changes and cellular location of CD109 and TGFβ/SMAD pathway molecules in lumbar spinal cord of SOD1-G93A mice, and explored the role and mechanism of CD109 on LPS-treated BV2 microglia and primary microglia derived from SOD1-G93A mice. Our results showed an increased expression of CD109 and TGFβ/SMAD pathway molecules in lumbar spinal cord of SOD1-G93A mice. Further cellular localization analysis demonstrated that proliferating microglia contributed mainly to the upregulation of CD109 and TGFβ1. Moreover, CD109 intervention in vitro partially reduced inflammatory response and TGFβ/SMAD pathway activation in both LPS-treated BV2 microglia and primary SOD1-G93A microglia. Thus, CD109 was involved in pathogenesis of ALS mice, and interventions targeting on CD109 modulation could be a potential therapeutic strategy for ALS.

CD109 是一种多功能核心受体，其在肿瘤进展和转移中的功能已被广泛研究。据报道，CD109的主要作用之一是下调TGFβ信号传导。然而，CD109在中枢神经系统，尤其是神经退行性疾病中的作用却鲜为人知。在此，我们研究了CD109和TGFβ/SMAD通路分子在SOD1-G93A小鼠腰脊髓中的表达变化和细胞位置，并探讨了CD109在经LPS处理的BV2小胶质细胞和SOD1-G93A小鼠原代小胶质细胞中的作用和机制。结果表明，SOD1-G93A 小鼠腰脊髓中 CD109 和 TGFβ/SMAD 通路分子的表达增加。进一步的细胞定位分析表明，增殖的小胶质细胞主要导致了 CD109 和 TGFβ1 的上调。此外，体外干预 CD109 可部分减少 LPS 处理的 BV2 小胶质细胞和原代 SOD1-G93A 小胶质细胞的炎症反应和 TGFβ/SMAD 通路激活。因此，CD109参与了ALS小鼠的发病机制，针对CD109调节的干预措施可能是ALS的一种潜在治疗策略。

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引用次数: 0

Aberrant intra-network resting-state functional connectivity in chronic insomnia with or without cognitive impairment. 伴有或不伴有认知障碍的慢性失眠症患者网络内静息态功能连接异常。

IF 2.9 3区医学 Q2 NEUROSCIENCES

Neuroscience

Pub Date : 2024-11-21 DOI: 10.1016/j.neuroscience.2024.11.046

Jurong Ding, Mengjie Yu, Lihong Li, Mei Yang, Pan Yang, Bo Hua, Xin Ding

Chronic insomnia (CI) is a common sleep disorder in middle-aged and elderly individuals. Long-term sleep deprivation can lead to physical, mental, and cognitive damage. Resting-state networks (RSNs) in the brain are closely linked to cognition and behavior. Therefore, we investigated changes in RSNs to explore behavioral and cognitive abnormalities in middle-aged and elderly CI patients. Resting state functional magnetic resonance imaging (rs-fMRI) and independent component analysis were used to study the intrinsic functional connectivity (FC) of the RSNs in 36 CI patients (20 CI with cognitive impairment (CI-I) patients and 16 CI without cognitive impairment (CI-N) patients) and 20 healthy controls (HC). Two-sample t-tests were used to compare RSNs differences between CI and HC groups and the RSNs differences between CI-I and CI-N groups. Partial correlation analysis was used to explore the relationship between the significant abnormal brain regions in RSN and clinical scales. Compared with HCs, CI patients showed significant differences in multiple RSNs, and FC values in two brain regions within RSNs were correlated with clinical scales. Furthermore, compared with CI-N group, CI-I group also showed significantly altered FC in multiple RSNs. Moreover, FC values in the right middle frontal gyrus within right frontal parietal network of CI-I patients were negatively correlated with the Mini-Mental State Examination scores. These results may explain hyperarousal, decreased attention and motor function impairments in CI patients. Furthermore, the aberrant alterations of RSNs in CI-I patients may play a crucial role in the onset and progression of cognitive impairment in CI patients.

慢性失眠（CI）是中老年人常见的睡眠障碍。长期睡眠不足会导致身体、精神和认知能力受损。大脑中的静息态网络（RSN）与认知和行为密切相关。因此，我们研究了 RSNs 的变化，以探讨中老年 CI 患者的行为和认知异常。我们采用静息状态功能磁共振成像（rs-fMRI）和独立成分分析法研究了 36 名 CI 患者（20 名有认知障碍的 CI 患者（CI-I）和 16 名无认知障碍的 CI 患者（CI-N））和 20 名健康对照组（HC）中 RSN 的内在功能连接性（FC）。采用双样本 t 检验比较 CI 组和 HC 组之间的 RSNs 差异，以及 CI-I 组和 CI-N 组之间的 RSNs 差异。偏相关分析用于探讨 RSN 中显著异常脑区与临床量表之间的关系。与 HC 组相比，CI 患者的多个 RSN 存在显著差异，且 RSN 中两个脑区的 FC 值与临床量表相关。此外，与CI-N组相比，CI-I组在多个RSN中的FC值也有明显变化。此外，CI-I 组患者右额顶叶网络中的右额中部回的 FC 值与迷你精神状态检查评分呈负相关。这些结果可以解释 CI-I 患者的过度焦虑、注意力下降和运动功能障碍。此外，CI-Ⅰ 患者 RSN 的异常改变可能在 CI 患者认知功能障碍的发生和发展过程中起着至关重要的作用。

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引用次数: 0

Differentiated and mature neurons are more responsive to neurotoxicant exposure at both transcriptional and translational levels 分化和成熟的神经元在转录和翻译水平上对暴露于神经毒素的反应更为敏感。

IF 2.9 3区医学 Q2 NEUROSCIENCES

Neuroscience

Pub Date : 2024-11-20 DOI: 10.1016/j.neuroscience.2024.11.017

Sana Sarkar , Anuj Pandey , Sanjeev Kumar Yadav , Mohammed Haris Siddiqui , A.B. Pant , Sanjay Yadav

SH-SY5Y human neuroblastoma cells have been extensively used as an in vitro model system in a diverse range of studies involving neurodevelopment, neurotoxicity, neurodegeneration, and neuronal ageing. Both naïve and differentiated phenotypes of SH-SY5Y cells are utilized to model human neurons under in vitro conditions. The process of differentiation causes extensive remodeling of neuronal cells at multiple omic levels, including the epigenome and proteome. In the present investigation, the miRNAome and proteome profiles of arsenic-treated naïve and differentiated SH-SY5Y cells were generated using the miRNA OpenArray technology and high-resolution mass spectrometry. Our findings demonstrated that differentiation dramatically affected the response of SH-SY5Y cells to toxicant exposure, as indicated by increased tolerance of differentiated cells against arsenic exposure compared to naïve cells in cell viability assay. Arsenic-exposed naïve and differentiated SH-SY5Y cells possess distinct miRNA and protein profiles with few similarities. Compared to naïve cells, differentiated cells have undergone higher deregulation in the expression of brain-enriched miRNAs and proteins and have shown a more drastic decrease in oxygen consumption rate, which is a measure of mitochondrial respiration after exposure to arsenic. Proteins identified in arsenic-treated differentiated SH-SY5Y cells were more enriched in pathways underlying multifactorial neurotoxic events. Additionally, more functional regulatory modules have been identified between the miRNAs and proteins differentially expressed in arsenic-treated differentiated SH-SY5Y cells relative to naïve cells. Collectively, our studies have shown that differentiated SH-SY5Y cells displayed alterations in the expression of a greater number of miRNAs and proteins following neurotoxicant exposure, indicating their higher responsivity.

在涉及神经发育、神经毒性、神经变性和神经元老化的各种研究中，SH-SY5Y 人神经母细胞瘤细胞已被广泛用作体外模型系统。在体外条件下，SH-SY5Y 细胞的幼稚和分化表型都被用来模拟人类神经元。分化过程会导致神经元细胞在包括表观基因组和蛋白质组在内的多个微观层面发生广泛重塑。本研究利用 miRNA OpenArray 技术和高分辨率质谱技术，生成了砷处理过的天真和分化的 SH-SY5Y 细胞的 miRNA 组和蛋白质组图谱。我们的研究结果表明，分化极大地影响了 SH-SY5Y 细胞对毒物暴露的反应，在细胞活力测定中，分化细胞对砷暴露的耐受性高于幼稚细胞。砷暴露的幼稚细胞和分化的 SH-SY5Y 细胞具有不同的 miRNA 和蛋白质图谱，几乎没有相似之处。与天真细胞相比，分化细胞的脑丰富 miRNA 和蛋白质的表达发生了更大程度的失调，而且耗氧率（衡量砷暴露后线粒体呼吸的指标）也出现了更大幅度的下降。在砷处理过的分化型 SH-SY5Y 细胞中发现的蛋白质在多因素神经毒性事件的通路中更为丰富。此外，与天真细胞相比，在砷处理的分化 SH-SY5Y 细胞中差异表达的 miRNA 和蛋白质之间发现了更多的功能调控模块。总之，我们的研究表明，分化的 SH-SY5Y 细胞在暴露于神经毒物后会出现更多 miRNA 和蛋白质表达的改变，这表明它们具有更高的反应性。

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引用次数: 0

Resting-state voxel-wise dynamic effective connectivity predicts risky decision-making in patients with bipolar disorder type I 静息态体素动态有效连通性可预测双相情感障碍 I 型患者的风险决策。

IF 2.9 3区医学 Q2 NEUROSCIENCES

Neuroscience

Pub Date : 2024-11-20 DOI: 10.1016/j.neuroscience.2024.11.024

Shanling Ji , Hongyong Zhang , Cong Zhou , Xia Liu , Chuanxin Liu , Hao Yu

Patients with Bipolar Disorder type I (BD-I) exhibit maladaptive risky decision-making, which is related to impulsivity, suicide attempts, and aggressive behavior. Currently, there is a lack of effective predictive methods for early intervention in risky behaviors for patients with BD-I. This study aimed to predict risky behavior in patients with BD-I using resting-state functional magnetic resonance imaging (rs-fMRI). We included 48 patients with BD-I and 124 healthy controls (HC) and constructed voxel-wise functional connectivity (FC), dynamic FC (dFC), effective connectivity (EC), and dynamic EC (dEC) for each subject. The Balloon Analogue Risk Task (BART) was employed to measure the risky decision-making of all participants. We applied connectome-based predictive modeling (CPM) with five regression algorithms to predict risky behaviors as well as Barratt Impulsivity Scale (BIS) scores. Results showed that the BD-I had significantly lower risky adjusted pump scores compared to HC. The dEC-based linear regression-CPM model exhibited significant predictive ability for the adjusted pump scores in BD-I, while no significant predictive power was observed in HC. Furthermore, this model successfully predicted non-planning impulsiveness, motor impulsiveness, and BIS total score, but failed for attentional impulsiveness in BD-I. These findings provide a foundation for future work in predicting risky behaviors of psychiatric patients by using voxel-wise dEC underlying resting state.

躁郁症 I 型（BD-I）患者表现出适应不良的风险决策，这与冲动、自杀企图和攻击行为有关。目前，尚缺乏有效的预测方法对躁狂症 I 型患者的危险行为进行早期干预。本研究旨在利用静息态功能磁共振成像（rs-fMRI）预测 BD-I 患者的危险行为。我们纳入了 48 名 BD-I 患者和 124 名健康对照（HC），并为每个受试者构建了体素功能连通性（FC）、动态 FC（dFC）、有效连通性（EC）和动态 EC（dEC）。我们采用气球模拟风险任务（BART）来测量所有受试者的风险决策能力。我们采用了基于连接组的预测模型（CPM）和五种回归算法来预测危险行为和巴拉特冲动量表（BIS）得分。结果显示，与 HC 相比，BD-I 的风险调整泵得分明显较低。基于 dEC 的线性回归-CPM 模型对 BD-I 的调整后泵评分具有显著的预测能力，而对 HC 则没有显著的预测能力。此外，该模型成功预测了 BD-I 的非计划冲动、运动冲动和 BIS 总分，但未能预测注意力冲动。这些发现为今后利用静息状态下的体素密度预测精神病患者的危险行为奠定了基础。

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