Oleosomes, naturally occurring plant-based oil droplets, have gained prominence as versatile emulsifying agents when combined with proteins in food applications. However, the extraction efficiency and physical stability of oleosome and protein mixture extracted from rapeseeds can be affected by the extraction method. Thus, this study compares the centrifugation and cheesecloth filtration methods on the composition, yield, the physico-chemical properties, and the storage stability of oleosome–protein mixtures. Results showed a higher oleosome and protein extraction yield (77 and 57 w/w%) by filtration method compared to centrifugation (70 and 50 w/w%). The oleosome and protein concentrations in the mixtures, as well as viscosity, remained comparable between the two methods. Filtration could enhance the stability of oleosome–protein mixtures compared to centrifugation, irrespective of pH variations and heating treatment at 90°C for 15 min. This stability improvement was attributed to differences in energy density resulting from mechanical forces rather than viscosity and composition. The study not only highlights the efficiency of filtration in optimizing extraction yields but also underscores its potential to enhance the storage stability of oleosome–protein mixtures, which can be beneficial for cost-effectiveness and environmental impact with less energy input compared to the centrifugation process.
{"title":"Comparative Assessment of Extraction Efficiency and Physical Stability of Rapeseed Oleosome–Protein Mixtures via Centrifugation Versus Cheesecloth Filtration","authors":"Chenqiang Qin, Rao Fu, Xin Wen, Yuanying Ni, Remko Marcel Boom, Constantinos V. Nikiforidis","doi":"10.1111/1750-3841.70214","DOIUrl":"https://doi.org/10.1111/1750-3841.70214","url":null,"abstract":"<div>\u0000 \u0000 <p>Oleosomes, naturally occurring plant-based oil droplets, have gained prominence as versatile emulsifying agents when combined with proteins in food applications. However, the extraction efficiency and physical stability of oleosome and protein mixture extracted from rapeseeds can be affected by the extraction method. Thus, this study compares the centrifugation and cheesecloth filtration methods on the composition, yield, the physico-chemical properties, and the storage stability of oleosome–protein mixtures. Results showed a higher oleosome and protein extraction yield (77 and 57 w/w%) by filtration method compared to centrifugation (70 and 50 w/w%). The oleosome and protein concentrations in the mixtures, as well as viscosity, remained comparable between the two methods. Filtration could enhance the stability of oleosome–protein mixtures compared to centrifugation, irrespective of pH variations and heating treatment at 90°C for 15 min. This stability improvement was attributed to differences in energy density resulting from mechanical forces rather than viscosity and composition. The study not only highlights the efficiency of filtration in optimizing extraction yields but also underscores its potential to enhance the storage stability of oleosome–protein mixtures, which can be beneficial for cost-effectiveness and environmental impact with less energy input compared to the centrifugation process.</p>\u0000 </div>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":"90 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lu Zhang, Mei Deng, Qinghui Wen, Yutong Xie, Qiao Ding, Xing Xie, Quanyuan Xie, Mingshun Chen
Nowadays, obesity is a global health risk factor, and its development is closely related to the absorption and metabolism of lipids. In this study, the main chemical constituents and antiobesity effect of Artemisia selengensis Turcz extract (ASTE) were investigated by HPLC-QTOF-MS2 and obese mice models. Twenty-three compounds were identified from ASTE, and caffeoylquinic acid and dicaffeoylquinic acid are the dominant bioactive compounds. ASTE administration reduced body weight (9.8%), improved glucose tolerance (14.2%), corrected dyslipidemia (the levels of total cholesterol, total triglyceride, and low-density lipoprotein cholesterol dropped by 19.8%, 24.4%, and 27.2%, respectively, and the high-density lipoprotein cholesterol level rose by 27.6%), and alleviated hepatic lipid accumulation. ASTE improved the gut microbiota dysbiosis caused by High-Fat Diet (HFD), mainly by increasing the relative abundance of Odoribacter, Candidatus_Saccharimonas, Bacteroides, and unclassified_f__Lachnospiraceae, and reducing the relative abundance of Faecalibaculum. Gene expression heatmaps and pathway enrichment analyses based on transcriptomics indicated that ASTE significantly reduced HFD-induced increases in fatty acid uptake, triglyceride synthesis, and cholesterol synthesis. Our findings indicated that ASTE holds significant potential as a candidate for modulating lipid metabolism and preventing or treating obesity, meriting further investigation.
{"title":"Effect of Artemisia selengensis Turcz extract on lipid metabolism and gut microbiota in high-fat diet-induced C57BL/6J obese mice","authors":"Lu Zhang, Mei Deng, Qinghui Wen, Yutong Xie, Qiao Ding, Xing Xie, Quanyuan Xie, Mingshun Chen","doi":"10.1111/1750-3841.70162","DOIUrl":"https://doi.org/10.1111/1750-3841.70162","url":null,"abstract":"<p>Nowadays, obesity is a global health risk factor, and its development is closely related to the absorption and metabolism of lipids. In this study, the main chemical constituents and antiobesity effect of <i>Artemisia selengensis</i> Turcz extract (ASTE) were investigated by HPLC-QTOF-MS<sup>2</sup> and obese mice models. Twenty-three compounds were identified from ASTE, and caffeoylquinic acid and dicaffeoylquinic acid are the dominant bioactive compounds. ASTE administration reduced body weight (9.8%), improved glucose tolerance (14.2%), corrected dyslipidemia (the levels of total cholesterol, total triglyceride, and low-density lipoprotein cholesterol dropped by 19.8%, 24.4%, and 27.2%, respectively, and the high-density lipoprotein cholesterol level rose by 27.6%), and alleviated hepatic lipid accumulation. ASTE improved the gut microbiota dysbiosis caused by High-Fat Diet (HFD), mainly by increasing the relative abundance of <i>Odoribacter</i>, <i>Candidatus_Saccharimonas</i>, <i>Bacteroides</i>, and <i>unclassified_f__Lachnospiraceae</i>, and reducing the relative abundance of <i>Faecalibaculum</i>. Gene expression heatmaps and pathway enrichment analyses based on transcriptomics indicated that ASTE significantly reduced HFD-induced increases in fatty acid uptake, triglyceride synthesis, and cholesterol synthesis. Our findings indicated that ASTE holds significant potential as a candidate for modulating lipid metabolism and preventing or treating obesity, meriting further investigation.</p>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":"90 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143857061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Serhat Al, Adam J. Franzen, Koeun Hwang, Robert E. Campbell, Kathleen Glass, Kurt D. Vogel, James R. Claus
The ability of carcass vascular rinsing supplemented with bacteriophage (BP) and peracetic acid (PAA) to reduce Salmonella in lymph nodes (LNs) from experimentally infected goats was determined. Cull dairy goats (n = 60) were randomly assigned to a control (CN, nonrinsed) and two vascular rinse treatments: BP and PAA. Goats were inoculated intradermally with Salmonella Enteritidis and slaughtered after a 7-day incubation. Vascular rinsing was performed postexsanguination via a catheter in the heart. Carcasses were skinned, eviscerated, sprayed with 5% lactic acid, and chilled (2°C) overnight. The superficial cervical, popliteal, medial iliac, and subiliac LNs were collected aseptically for Salmonella enumeration and phage titer determination. The longissimus dorsi (LD) and semimembranosus muscles were also excised, and stored for 1, 4, and 7 days for meat surface color measurements. PAA-treated carcasses showed lower (p < 0.05) temperatures and pH values within 8 h postmortem compared to CN and BP. The average counts of Salmonella in the LNs associated with PAA (3.4 ± 1.3 log CFU/g) were significantly lower compared to CN (3.8 ± 1.1 log CFU/g), with the lowest load observed in medial iliac LNs (2.7 ± 1.5 log CFU/g). Substantial phage titers were detected in LNs from BP-treated carcasses (7.0 ± 0.91 log PFU/g), and no differences were observed in Salmonella counts in BP compared to CN. The meat samples obtained from PAA-treated carcasses exhibited lower redness (a* values) and deoxymyoglobin in the LD (p < 0.05) but showed no differences in lightness or oxymyoglobin compared to BP and CN. Vascular rinsing has the potential to suppress Salmonella in the LNs with other antimicrobials and chemicals with different combinations and concentrations.
Practical Application
This study investigates a method to control Salmonella in goat meat by rinsing carcasses with peracetic acid (PAA) or bacteriophages through the bloodstream after slaughter. The findings suggest that PAA can mitigate Salmonella levels in lymph nodes, potentially improving meat safety. While bacteriophage treatment did not significantly affect the bacterial count to observe differences with the control group, vascular rinsing could still be promising with different antimicrobial combinations. This research would help meat processors enhance food safety measures, reducing the risk of Salmonella contamination in meat products.
{"title":"Comparison of peracetic acid and bacteriophage application by vascular rinsing on Salmonella reduction in lymph nodes of goat carcasses","authors":"Serhat Al, Adam J. Franzen, Koeun Hwang, Robert E. Campbell, Kathleen Glass, Kurt D. Vogel, James R. Claus","doi":"10.1111/1750-3841.70203","DOIUrl":"https://doi.org/10.1111/1750-3841.70203","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 \u0000 <p>The ability of carcass vascular rinsing supplemented with bacteriophage (BP) and peracetic acid (PAA) to reduce <i>Salmonella</i> in lymph nodes (LNs) from experimentally infected goats was determined. Cull dairy goats (<i>n</i> = 60) were randomly assigned to a control (CN, nonrinsed) and two vascular rinse treatments: BP and PAA. Goats were inoculated intradermally with <i>Salmonella</i> Enteritidis and slaughtered after a 7-day incubation. Vascular rinsing was performed postexsanguination via a catheter in the heart. Carcasses were skinned, eviscerated, sprayed with 5% lactic acid, and chilled (2°C) overnight. The superficial cervical, popliteal, medial iliac, and subiliac LNs were collected aseptically for <i>Salmonella</i> enumeration and phage titer determination. The longissimus dorsi (LD) and semimembranosus muscles were also excised, and stored for 1, 4, and 7 days for meat surface color measurements. PAA-treated carcasses showed lower (<i>p </i>< 0.05) temperatures and pH values within 8 h postmortem compared to CN and BP. The average counts of <i>Salmonella</i> in the LNs associated with PAA (3.4 ± 1.3 log CFU/g) were significantly lower compared to CN (3.8 ± 1.1 log CFU/g), with the lowest load observed in medial iliac LNs (2.7 ± 1.5 log CFU/g). Substantial phage titers were detected in LNs from BP-treated carcasses (7.0 ± 0.91 log PFU/g), and no differences were observed in <i>Salmonella</i> counts in BP compared to CN. The meat samples obtained from PAA-treated carcasses exhibited lower redness (a* values) and deoxymyoglobin in the LD (<i>p </i>< 0.05) but showed no differences in lightness or oxymyoglobin compared to BP and CN. Vascular rinsing has the potential to suppress <i>Salmonella</i> in the LNs with other antimicrobials and chemicals with different combinations and concentrations.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Practical Application</h3>\u0000 \u0000 <p>This study investigates a method to control <i>Salmonella</i> in goat meat by rinsing carcasses with peracetic acid (PAA) or bacteriophages through the bloodstream after slaughter. The findings suggest that PAA can mitigate <i>Salmonella</i> levels in lymph nodes, potentially improving meat safety. While bacteriophage treatment did not significantly affect the bacterial count to observe differences with the control group, vascular rinsing could still be promising with different antimicrobial combinations. This research would help meat processors enhance food safety measures, reducing the risk of <i>Salmonella</i> contamination in meat products.</p>\u0000 </section>\u0000 </div>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":"90 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1750-3841.70203","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143857062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiaofang Bai, Si Hong Park, Robert McGorrin, Yanyun Zhao, Jooyeoun Jung
This study evaluated the thermal inactivation properties of Aspergillus flavus (A. flavus) and Aspergillus oryzae (A. oryzae) in hazelnut kernels and shells to assess the suitability of using A. oryzae as a surrogate and explored the potential of radiofrequency (RF) dielectric heating to control Aspergillus mold. Both molds exhibited similar heat sensitivities at 45, 55, and 65°C, achieving complete inactivation at 65°C without holding time, though resistance persisted at 45°C even with prolonged heating. Notably, A. flavus was 5°C more heat-resistant and more sensitive to holding time compared with A. oryzae. The critical inactivation temperature was 55°C for A. oryzae and 60°C for A. flavus. At 50°C, A. oryzae displayed comparable thermal resistance in hazelnut shell and kernel powders, but its behavior diverged at 55°C. While A. oryzae reduction was observed in shells during inoculation, no reduction occurred in kernels. RF heating achieved a 0.48-log reduction in A. oryzae in inoculated in-shell hazelnuts when the kernel temperature reached 70°C, increasing to a 0.81-log reduction when the shell temperature reached 65°C. The incomplete inactivation on the shell surface might be attributed to the low moisture content and poor thermal conductivity of the shells. Lipid oxidation of RF-heated kernels was evaluated by fatty acid content and K values, and no significant differences were observed from the unheated samples. These findings highlight the potential of using A. oryzae as a surrogate of A. flavus and RF heating to inactivate Aspergillus to ensure hazelnut safety and prevent lipid deterioration.
{"title":"Evaluation of Aspergillus oryzae as a Surrogate of Aspergillus flavus and Radiofrequency Dielectric Heating to Control Aspergillus Mold in Inshell Hazelnuts","authors":"Xiaofang Bai, Si Hong Park, Robert McGorrin, Yanyun Zhao, Jooyeoun Jung","doi":"10.1111/1750-3841.70211","DOIUrl":"https://doi.org/10.1111/1750-3841.70211","url":null,"abstract":"<div>\u0000 \u0000 <p>This study evaluated the thermal inactivation properties of <i>Aspergillus flavus</i> (<i>A. flavus</i>) and <i>Aspergillus oryzae</i> (<i>A. oryzae</i>) in hazelnut kernels and shells to assess the suitability of using <i>A. oryzae</i> as a surrogate and explored the potential of radiofrequency (RF) dielectric heating to control <i>Aspergillus</i> mold. Both molds exhibited similar heat sensitivities at 45, 55, and 65°C, achieving complete inactivation at 65°C without holding time, though resistance persisted at 45°C even with prolonged heating. Notably, <i>A. flavus</i> was 5°C more heat-resistant and more sensitive to holding time compared with <i>A. oryzae</i>. The critical inactivation temperature was 55°C for <i>A. oryzae</i> and 60°C for <i>A. flavus</i>. At 50°C, <i>A. oryzae</i> displayed comparable thermal resistance in hazelnut shell and kernel powders, but its behavior diverged at 55°C. While <i>A. oryzae</i> reduction was observed in shells during inoculation, no reduction occurred in kernels. RF heating achieved a 0.48-log reduction in <i>A. oryzae</i> in inoculated in-shell hazelnuts when the kernel temperature reached 70°C, increasing to a 0.81-log reduction when the shell temperature reached 65°C. The incomplete inactivation on the shell surface might be attributed to the low moisture content and poor thermal conductivity of the shells. Lipid oxidation of RF-heated kernels was evaluated by fatty acid content and K values, and no significant differences were observed from the unheated samples. These findings highlight the potential of using <i>A. oryzae</i> as a surrogate of <i>A. flavus</i> and RF heating to inactivate <i>Aspergillus</i> to ensure hazelnut safety and prevent lipid deterioration.</p>\u0000 </div>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":"90 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of this study was to extract watermelon rind (WR) pectin (WRP) and watermelon rind polyphenols (WRE) and evaluate the effect of pectin/sodium alginate composite films (WRPSA) with or without WRE on strawberry preservation. WRP was extracted using citric acid, whereas WRE was obtained through ethyl acetate extraction. The extracted WRP was characterized for composition and structural properties, multi-angle laser light scattering (MALLS), Fourier Transform Infrared Spectroscopy (FTIR), differential scanning calorimetry (DSC), x-ray diffraction (XRD), ζ-potential analysis, and rheological measurements. The pectin/SA film (WRPSA) and pectin/SA composite film containing WRE (WRPESA) were prepared. Both films were analyzed for physical properties and structurally characterized. Strawberries were wrapped with either WRPSA, WRPESA, or no treatment (exposed) and stored under lightproof conditions at 4°C for 12 days. The results showed that WRP was a low-ester pectin (degree of methylation [DM] = 38.7%) with a molecular weight of 683 kDa. WRP had typical polysaccharide characteristic peaks and good thermal stability that could be used as a film-forming substrate. WRPESA showed better mechanical strength and antioxidant capacity than that of WRPSA, indicating that WRE showed synergistic effects on the improvement of composite films. Additionally, WRPESA effectively reduced strawberry softening, color deterioration, and weight loss, in addition to the delay in the dynamic changes in sugar content and pH and accumulation of malondialdehyde (MDA). WRPESA also helped maintain the total phenolic content of the strawberries. These findings suggest that watermelon byproducts could be valorized for sustainable food packaging, reducing both agricultural waste and reliance on synthetic materials.
Practical Application: This article confirmed the feasibility of a sequential extraction process for watermelon rind pectin and polyphenols, which can later be applied to industrial production lines to increase the utilization rate of watermelon rind. The practical application value of this research lies in the development of biodegradable packaging materials. By extracting pectin and polyphenols from watermelon rind, the resulting films can effectively extend the shelf life of strawberries and reduce food waste. Furthermore, this study promotes the utilization of agricultural waste, enhances the nutritional value of food, and provides new opportunities for local economic development. Additionally, it offers a scientific basis for advancing sustainable packaging technology and food preservation innovations, which is significant for environmental protection and healthy consumption.
{"title":"Serial Extraction of Pectin and Polyphenols From Watermelon Rind for Use in Strawberry Preservation Film","authors":"Xiaolin Jin, Yue Han, Qingshen Sun","doi":"10.1111/1750-3841.70216","DOIUrl":"https://doi.org/10.1111/1750-3841.70216","url":null,"abstract":"<div>\u0000 \u0000 <p>The aim of this study was to extract watermelon rind (WR) pectin (WRP) and watermelon rind polyphenols (WRE) and evaluate the effect of pectin/sodium alginate composite films (WRPSA) with or without WRE on strawberry preservation. WRP was extracted using citric acid, whereas WRE was obtained through ethyl acetate extraction. The extracted WRP was characterized for composition and structural properties, multi-angle laser light scattering (MALLS), Fourier Transform Infrared Spectroscopy (FTIR), differential scanning calorimetry (DSC), x-ray diffraction (XRD), ζ-potential analysis, and rheological measurements. The pectin/SA film (WRPSA) and pectin/SA composite film containing WRE (WRPESA) were prepared. Both films were analyzed for physical properties and structurally characterized. Strawberries were wrapped with either WRPSA, WRPESA, or no treatment (exposed) and stored under lightproof conditions at 4°C for 12 days. The results showed that WRP was a low-ester pectin (degree of methylation [DM] = 38.7%) with a molecular weight of 683 kDa. WRP had typical polysaccharide characteristic peaks and good thermal stability that could be used as a film-forming substrate. WRPESA showed better mechanical strength and antioxidant capacity than that of WRPSA, indicating that WRE showed synergistic effects on the improvement of composite films. Additionally, WRPESA effectively reduced strawberry softening, color deterioration, and weight loss, in addition to the delay in the dynamic changes in sugar content and pH and accumulation of malondialdehyde (MDA). WRPESA also helped maintain the total phenolic content of the strawberries. These findings suggest that watermelon byproducts could be valorized for sustainable food packaging, reducing both agricultural waste and reliance on synthetic materials.</p>\u0000 <p><b>Practical Application</b>: This article confirmed the feasibility of a sequential extraction process for watermelon rind pectin and polyphenols, which can later be applied to industrial production lines to increase the utilization rate of watermelon rind. The practical application value of this research lies in the development of biodegradable packaging materials. By extracting pectin and polyphenols from watermelon rind, the resulting films can effectively extend the shelf life of strawberries and reduce food waste. Furthermore, this study promotes the utilization of agricultural waste, enhances the nutritional value of food, and provides new opportunities for local economic development. Additionally, it offers a scientific basis for advancing sustainable packaging technology and food preservation innovations, which is significant for environmental protection and healthy consumption.</p>\u0000 </div>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":"90 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143857060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Da Shi, Ilekuttige Priyan Shanura Fernando, Amir Vahedifar, Supratim Ghosh, Jianping Wu
Osteoporosis, the most prevalent metabolic bone disorder, is a major public health issue. Previous studies indicated the potential of food components in mitigating the risks of osteoporosis. The study aimed to evaluate the potential of canola protein hydrolysates (CPH) on osteoclastogenesis using a pre-osteoblast cell MC3T3-E1. Twenty-two CPHs were prepared by 12 different proteases, either individually or in combination. Three CPHs, prepared by trypsin (CPH-T), Protex 6L (CPH-P), and the combination of Protex 6L and thermoase (CPH-PT) showed promising activity in promoting in vitro bone formation. CPH-T and CPH-PT improved cell proliferation at a concentration of 10 ug/ml, while all three hydrolysates exhibited cytotoxicity at 1000 ug/ml. All three hydrolysates promoted the level of runt-related transcription factor 2 (RUNX2) and type I collagen, and mineralization in osteoblast cells, in a dose-dependent manner. Additionally, these three hydrolysates elevated the osteoprotegerin (OPG) level and reduced the level of receptor activator of nuclear factor kappa-B ligand (RANKL). This study indicated the activity of CPHs in the promotion of bone formation and prevention of osteoclastogenesis, suggesting the potential of CPHs as a promising functional food ingredient against osteoporosis.
{"title":"Canola Protein Hydrolysates Show Osteogenic Activity in MC3T3-E1 Cells","authors":"Da Shi, Ilekuttige Priyan Shanura Fernando, Amir Vahedifar, Supratim Ghosh, Jianping Wu","doi":"10.1111/1750-3841.70210","DOIUrl":"https://doi.org/10.1111/1750-3841.70210","url":null,"abstract":"<p>Osteoporosis, the most prevalent metabolic bone disorder, is a major public health issue. Previous studies indicated the potential of food components in mitigating the risks of osteoporosis. The study aimed to evaluate the potential of canola protein hydrolysates (CPH) on osteoclastogenesis using a pre-osteoblast cell MC3T3-E1. Twenty-two CPHs were prepared by 12 different proteases, either individually or in combination. Three CPHs, prepared by trypsin (CPH-T), Protex 6L (CPH-P), and the combination of Protex 6L and thermoase (CPH-PT) showed promising activity in promoting in vitro bone formation. CPH-T and CPH-PT improved cell proliferation at a concentration of 10 ug/ml, while all three hydrolysates exhibited cytotoxicity at 1000 ug/ml. All three hydrolysates promoted the level of runt-related transcription factor 2 (RUNX2) and type I collagen, and mineralization in osteoblast cells, in a dose-dependent manner. Additionally, these three hydrolysates elevated the osteoprotegerin (OPG) level and reduced the level of receptor activator of nuclear factor kappa-B ligand (RANKL). This study indicated the activity of CPHs in the promotion of bone formation and prevention of osteoclastogenesis, suggesting the potential of CPHs as a promising functional food ingredient against osteoporosis.</p>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":"90 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1750-3841.70210","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lu Zhang, Mei Deng, Qinghui Wen, Yutong Xie, Qiao Ding, Xing Xie, Quanyuan Xie, Mingshun Chen
Nowadays, obesity is a global health risk factor, and its development is closely related to the absorption and metabolism of lipids. In this study, the main chemical constituents and antiobesity effect of Artemisia selengensis Turcz extract (ASTE) were investigated by HPLC-QTOF-MS2 and obese mice models. Twenty-three compounds were identified from ASTE, and caffeoylquinic acid and dicaffeoylquinic acid are the dominant bioactive compounds. ASTE administration reduced body weight (9.8%), improved glucose tolerance (14.2%), corrected dyslipidemia (the levels of total cholesterol, total triglyceride, and low-density lipoprotein cholesterol dropped by 19.8%, 24.4%, and 27.2%, respectively, and the high-density lipoprotein cholesterol level rose by 27.6%), and alleviated hepatic lipid accumulation. ASTE improved the gut microbiota dysbiosis caused by High-Fat Diet (HFD), mainly by increasing the relative abundance of Odoribacter, Candidatus_Saccharimonas, Bacteroides, and unclassified_f__Lachnospiraceae, and reducing the relative abundance of Faecalibaculum. Gene expression heatmaps and pathway enrichment analyses based on transcriptomics indicated that ASTE significantly reduced HFD-induced increases in fatty acid uptake, triglyceride synthesis, and cholesterol synthesis. Our findings indicated that ASTE holds significant potential as a candidate for modulating lipid metabolism and preventing or treating obesity, meriting further investigation.
如今,肥胖已成为全球性的健康风险因素,而肥胖的发生与脂质的吸收和代谢密切相关。本研究通过HPLC-QTOF-MS2和肥胖小鼠模型研究了艾蒿提取物(ASTE)的主要化学成分和抗肥胖作用。从 ASTE 中鉴定出 23 种化合物,其中咖啡酰奎宁酸和二咖啡酰奎宁酸是主要的生物活性化合物。服用 ASTE 可降低体重(9.8%),改善葡萄糖耐量(14.2%),纠正血脂异常(总胆固醇、总甘油三酯和低密度脂蛋白胆固醇水平分别下降 19.8%、24.4% 和 27.2%,高密度脂蛋白胆固醇水平上升 27.6%),缓解肝脏脂质堆积。ASTE改善了高脂饮食(HFD)引起的肠道微生物群失调,主要是通过增加Odoribacter、Candidatus_Saccharimonas、Bacteroides和unclassified__f__Lachnospiraceae的相对丰度,降低Faecalibaculum的相对丰度。基于转录组学的基因表达热图和通路富集分析表明,ASTE 能显著降低 HFD 诱导的脂肪酸摄取、甘油三酯合成和胆固醇合成的增加。我们的研究结果表明,ASTE 作为调节脂质代谢、预防或治疗肥胖症的候选物质具有巨大潜力,值得进一步研究。
{"title":"Effect of Artemisia selengensis Turcz extract on lipid metabolism and gut microbiota in high-fat diet-induced C57BL/6J obese mice","authors":"Lu Zhang, Mei Deng, Qinghui Wen, Yutong Xie, Qiao Ding, Xing Xie, Quanyuan Xie, Mingshun Chen","doi":"10.1111/1750-3841.70162","DOIUrl":"https://doi.org/10.1111/1750-3841.70162","url":null,"abstract":"<p>Nowadays, obesity is a global health risk factor, and its development is closely related to the absorption and metabolism of lipids. In this study, the main chemical constituents and antiobesity effect of <i>Artemisia selengensis</i> Turcz extract (ASTE) were investigated by HPLC-QTOF-MS<sup>2</sup> and obese mice models. Twenty-three compounds were identified from ASTE, and caffeoylquinic acid and dicaffeoylquinic acid are the dominant bioactive compounds. ASTE administration reduced body weight (9.8%), improved glucose tolerance (14.2%), corrected dyslipidemia (the levels of total cholesterol, total triglyceride, and low-density lipoprotein cholesterol dropped by 19.8%, 24.4%, and 27.2%, respectively, and the high-density lipoprotein cholesterol level rose by 27.6%), and alleviated hepatic lipid accumulation. ASTE improved the gut microbiota dysbiosis caused by High-Fat Diet (HFD), mainly by increasing the relative abundance of <i>Odoribacter</i>, <i>Candidatus_Saccharimonas</i>, <i>Bacteroides</i>, and <i>unclassified_f__Lachnospiraceae</i>, and reducing the relative abundance of <i>Faecalibaculum</i>. Gene expression heatmaps and pathway enrichment analyses based on transcriptomics indicated that ASTE significantly reduced HFD-induced increases in fatty acid uptake, triglyceride synthesis, and cholesterol synthesis. Our findings indicated that ASTE holds significant potential as a candidate for modulating lipid metabolism and preventing or treating obesity, meriting further investigation.</p>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":"90 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Developing a rapid, efficient, and environmentally friendly analytical methodology for the determination of aromatic esters (AREs) in Baijiu presents a significant challenge. In this study, we developed a room-temperature method to synthesize core-shell Fe3O4@TPB-Dha in 1 h using pre-prepared Fe3O4 nanoparticles, enabling rapid and sensitive detection of AREs. The Fe3O4@TPB-Dha demonstrated exceptional adsorption capabilities (52.63–58.82 mg/g) with a brief adsorption duration (4 min) for four AREs as magnetic solid phase extraction (MSPE) adsorbent. Moreover, density functional theory (DFT) calculations revealed the key adsorption mechanisms to be hydrogen bonds, van der Waals forces, and π–π interactions. Ultimately, the method enabled the rapid and accurate quantification of the four AREs in real Baijiu samples, with concentrations spanning from 0.17 ± 6 × 10−4 to 2.40 ± 0.03 mg/L, and exhibited relative standard deviations below 6.57% (n = 3). These findings suggest that Fe3O4@TPB-Dha holds promise as an effective adsorbent for efficient extraction and precise quantification of aromatic esters in Baijiu.
{"title":"Novel Room-Temperature-Prepared Covalent Organic Framework as Adsorbent for Rapid Extraction of Aromatic Esters in Baijiu","authors":"Yongqing Zhang, Zhen Fan, Xiaolong Shang, Ling Ao, Miao Liu, Feng Lin, Baoguo Sun, Wei Dong, Xiaotao Sun, Yanfei Xiong, Bo Deng","doi":"10.1111/1750-3841.70215","DOIUrl":"https://doi.org/10.1111/1750-3841.70215","url":null,"abstract":"<div>\u0000 \u0000 <p>Developing a rapid, efficient, and environmentally friendly analytical methodology for the determination of aromatic esters (AREs) in Baijiu presents a significant challenge. In this study, we developed a room-temperature method to synthesize core-shell Fe<sub>3</sub>O<sub>4</sub>@TPB-Dha in 1 h using pre-prepared Fe<sub>3</sub>O<sub>4</sub> nanoparticles, enabling rapid and sensitive detection of AREs. The Fe<sub>3</sub>O<sub>4</sub>@TPB-Dha demonstrated exceptional adsorption capabilities (52.63–58.82 mg/g) with a brief adsorption duration (4 min) for four AREs as magnetic solid phase extraction (MSPE) adsorbent. Moreover, density functional theory (DFT) calculations revealed the key adsorption mechanisms to be hydrogen bonds, van der Waals forces, and π–π interactions. Ultimately, the method enabled the rapid and accurate quantification of the four AREs in real Baijiu samples, with concentrations spanning from 0.17 ± 6 × 10<sup>−4</sup> to 2.40 ± 0.03 mg/L, and exhibited relative standard deviations below 6.57% (<i>n</i> = 3). These findings suggest that Fe<sub>3</sub>O<sub>4</sub>@TPB-Dha holds promise as an effective adsorbent for efficient extraction and precise quantification of aromatic esters in Baijiu.</p>\u0000 </div>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":"90 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiaofang Bai, Si Hong Park, Robert McGorrin, Yanyun Zhao, Jooyeoun Jung
This study evaluated the thermal inactivation properties of Aspergillus flavus (A. flavus) and Aspergillus oryzae (A. oryzae) in hazelnut kernels and shells to assess the suitability of using A. oryzae as a surrogate and explored the potential of radiofrequency (RF) dielectric heating to control Aspergillus mold. Both molds exhibited similar heat sensitivities at 45, 55, and 65°C, achieving complete inactivation at 65°C without holding time, though resistance persisted at 45°C even with prolonged heating. Notably, A. flavus was 5°C more heat-resistant and more sensitive to holding time compared with A. oryzae. The critical inactivation temperature was 55°C for A. oryzae and 60°C for A. flavus. At 50°C, A. oryzae displayed comparable thermal resistance in hazelnut shell and kernel powders, but its behavior diverged at 55°C. While A. oryzae reduction was observed in shells during inoculation, no reduction occurred in kernels. RF heating achieved a 0.48-log reduction in A. oryzae in inoculated in-shell hazelnuts when the kernel temperature reached 70°C, increasing to a 0.81-log reduction when the shell temperature reached 65°C. The incomplete inactivation on the shell surface might be attributed to the low moisture content and poor thermal conductivity of the shells. Lipid oxidation of RF-heated kernels was evaluated by fatty acid content and K values, and no significant differences were observed from the unheated samples. These findings highlight the potential of using A. oryzae as a surrogate of A. flavus and RF heating to inactivate Aspergillus to ensure hazelnut safety and prevent lipid deterioration.
{"title":"Evaluation of Aspergillus oryzae as a Surrogate of Aspergillus flavus and Radiofrequency Dielectric Heating to Control Aspergillus Mold in Inshell Hazelnuts","authors":"Xiaofang Bai, Si Hong Park, Robert McGorrin, Yanyun Zhao, Jooyeoun Jung","doi":"10.1111/1750-3841.70211","DOIUrl":"https://doi.org/10.1111/1750-3841.70211","url":null,"abstract":"<div>\u0000 \u0000 <p>This study evaluated the thermal inactivation properties of <i>Aspergillus flavus</i> (<i>A. flavus</i>) and <i>Aspergillus oryzae</i> (<i>A. oryzae</i>) in hazelnut kernels and shells to assess the suitability of using <i>A. oryzae</i> as a surrogate and explored the potential of radiofrequency (RF) dielectric heating to control <i>Aspergillus</i> mold. Both molds exhibited similar heat sensitivities at 45, 55, and 65°C, achieving complete inactivation at 65°C without holding time, though resistance persisted at 45°C even with prolonged heating. Notably, <i>A. flavus</i> was 5°C more heat-resistant and more sensitive to holding time compared with <i>A. oryzae</i>. The critical inactivation temperature was 55°C for <i>A. oryzae</i> and 60°C for <i>A. flavus</i>. At 50°C, <i>A. oryzae</i> displayed comparable thermal resistance in hazelnut shell and kernel powders, but its behavior diverged at 55°C. While <i>A. oryzae</i> reduction was observed in shells during inoculation, no reduction occurred in kernels. RF heating achieved a 0.48-log reduction in <i>A. oryzae</i> in inoculated in-shell hazelnuts when the kernel temperature reached 70°C, increasing to a 0.81-log reduction when the shell temperature reached 65°C. The incomplete inactivation on the shell surface might be attributed to the low moisture content and poor thermal conductivity of the shells. Lipid oxidation of RF-heated kernels was evaluated by fatty acid content and K values, and no significant differences were observed from the unheated samples. These findings highlight the potential of using <i>A. oryzae</i> as a surrogate of <i>A. flavus</i> and RF heating to inactivate <i>Aspergillus</i> to ensure hazelnut safety and prevent lipid deterioration.</p>\u0000 </div>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":"90 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oleosomes, naturally occurring plant-based oil droplets, have gained prominence as versatile emulsifying agents when combined with proteins in food applications. However, the extraction efficiency and physical stability of oleosome and protein mixture extracted from rapeseeds can be affected by the extraction method. Thus, this study compares the centrifugation and cheesecloth filtration methods on the composition, yield, the physico-chemical properties, and the storage stability of oleosome–protein mixtures. Results showed a higher oleosome and protein extraction yield (77 and 57 w/w%) by filtration method compared to centrifugation (70 and 50 w/w%). The oleosome and protein concentrations in the mixtures, as well as viscosity, remained comparable between the two methods. Filtration could enhance the stability of oleosome–protein mixtures compared to centrifugation, irrespective of pH variations and heating treatment at 90°C for 15 min. This stability improvement was attributed to differences in energy density resulting from mechanical forces rather than viscosity and composition. The study not only highlights the efficiency of filtration in optimizing extraction yields but also underscores its potential to enhance the storage stability of oleosome–protein mixtures, which can be beneficial for cost-effectiveness and environmental impact with less energy input compared to the centrifugation process.
{"title":"Comparative Assessment of Extraction Efficiency and Physical Stability of Rapeseed Oleosome–Protein Mixtures via Centrifugation Versus Cheesecloth Filtration","authors":"Chenqiang Qin, Rao Fu, Xin Wen, Yuanying Ni, Remko Marcel Boom, Constantinos V. Nikiforidis","doi":"10.1111/1750-3841.70214","DOIUrl":"https://doi.org/10.1111/1750-3841.70214","url":null,"abstract":"<div>\u0000 \u0000 <p>Oleosomes, naturally occurring plant-based oil droplets, have gained prominence as versatile emulsifying agents when combined with proteins in food applications. However, the extraction efficiency and physical stability of oleosome and protein mixture extracted from rapeseeds can be affected by the extraction method. Thus, this study compares the centrifugation and cheesecloth filtration methods on the composition, yield, the physico-chemical properties, and the storage stability of oleosome–protein mixtures. Results showed a higher oleosome and protein extraction yield (77 and 57 w/w%) by filtration method compared to centrifugation (70 and 50 w/w%). The oleosome and protein concentrations in the mixtures, as well as viscosity, remained comparable between the two methods. Filtration could enhance the stability of oleosome–protein mixtures compared to centrifugation, irrespective of pH variations and heating treatment at 90°C for 15 min. This stability improvement was attributed to differences in energy density resulting from mechanical forces rather than viscosity and composition. The study not only highlights the efficiency of filtration in optimizing extraction yields but also underscores its potential to enhance the storage stability of oleosome–protein mixtures, which can be beneficial for cost-effectiveness and environmental impact with less energy input compared to the centrifugation process.</p>\u0000 </div>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":"90 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}