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Acceptable daily intake of aspartame aggravates enteritis pathology and systemic inflammation in colitis mouse model. 可接受的阿斯巴甜日摄入量会加重结肠炎小鼠模型的肠炎病理和全身炎症。
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-30 DOI: 10.1111/1750-3841.17505
Mengdan Zhong, Lifang Li, Wenhui Liu, Wenzhi Wen, Luheng Ma, Xiaobao Jin, Guoying Li, Junhua Yang

In this study, a dextran sodium sulfate-induced ulcerative colitis model in C57BL/6 mice was used to explore the effect of acceptable daily intake (ADI) of aspartame on inflammation in colonic tissues. The effects of aspartame on the inflammatory state of the colon in mice were comprehensively evaluated by comparing the body weight, colon length/colon length index, splenic index, disease activity index (DAI) score, histological activity index (HAI) score, the expression levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, claudin-3, and occludin, the infiltration characteristics of macrophage and neutrophil and the composition of the gut microbiota in the control group, aspartame group, ulcerative colitis model group, and aspartame + ulcerative colitis group. We demonstrated that, in a mouse model of dextran sulfate-induced ulcerative colitis, ADI of aspartame caused a significant decrease in body weight, colon length/colon length index, DAI scores, and expression levels of the proteins claudin-3 and occludin. Moreover, ADI of aspartame caused an increase in the splenic index, HAI scores, the levels of proinflammatory factors TNF-α, IL-1β, and IL-6 both in intestinal tissue and serum and infiltration of macrophages and neutrophils in colon tissues. These results showed that ADI of aspartame promoted intestinal pathology and systemic inflammation in a mouse model of colitis.

本研究采用右旋糖酐硫酸钠诱导的 C57BL/6 小鼠溃疡性结肠炎模型,探讨阿斯巴甜每日允许摄入量(ADI)对结肠组织炎症的影响。通过比较小鼠体重、结肠长度/结肠长度指数、脾脏指数、疾病活动指数(DAI)评分、组织学活动指数(HAI)评分、肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1表达水平,全面评估了阿斯巴甜对小鼠结肠炎症状态的影响、在对照组、阿斯巴甜组、溃疡性结肠炎模型组和阿斯巴甜+溃疡性结肠炎组中,我们观察了白细胞介素(IL)-1β、IL-6、Claudin-3 和闭塞素的表达水平、巨噬细胞和中性粒细胞的浸润特征以及肠道微生物群的组成。我们证实,在硫酸葡聚糖诱导的溃疡性结肠炎小鼠模型中,阿斯巴甜的每日摄入量会导致体重、结肠长度/结肠长度指数、DAI评分以及蛋白质claudin-3和occludin的表达水平显著下降。此外,阿斯巴甜的每日摄入量会导致脾脏指数、HAI评分、肠道组织和血清中促炎因子TNF-α、IL-1β和IL-6的水平以及结肠组织中巨噬细胞和中性粒细胞的浸润增加。这些结果表明,在小鼠结肠炎模型中,阿斯巴甜的每日允许摄入量会促进肠道病变和全身炎症。
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引用次数: 0
Exploring the relationship between field available water capacity (AWC) and atypical aging (ATA) development in base wines. 探索田间可用水量 (AWC) 与基酒非典型老化 (ATA) 发展之间的关系。
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-29 DOI: 10.1111/1750-3841.17500
Simone Delaiti, Tiziana Nardin, Tomas Roman, Nicola Cappello, Roberto Larcher, Stefano Pedò

Hydric stress is a leading cause of atypical aging (ATA) in wine, characterized by unpleasant olfactory notes. The main sensorial and chemical marker of ATA is 2-aminoacetophenone (AAP). Early detection of ATA before the second fermentation in sparkling wines (SWs) is crucial for producing high-quality products. Climate change-induced droughts significantly impact agriculture, including grape farming, particularly in vineyards with shallow soils and reduced available water capacity (AWC). This study examined the relationship between AWC and ATA in base wines (BWs) intended for SW production. Ten vineyards were classified into three AWC categories (low, medium, and high). Hydric stress levels were monitored over two growing seasons to explore their effects on vegeto-productive behavior and AAP development. During the first vintage, drought conditions led to potentially ATA-tainted BWs across all AWC classes. The impact varied with AWC, with low-AWC vineyards experiencing higher stress and producing BWs with elevated AAP levels and vegeto-productive imbalance. In contrast, the following season had unusual rainfall, resulting in some potentially ATA-affected BWs, but no significant differences in AAP content or vegeto-productive balance among the AWC classes were observed. In conclusion, grapevines on low-AWC soils are at a higher risk of producing faulty BWs, particularly in dry vintages.

水压力是葡萄酒非典型老化(ATA)的主要原因,其特征是令人不悦的嗅觉感受。ATA 的主要感官和化学标志是 2-氨基苯乙酮(AAP)。在起泡葡萄酒(SW)二次发酵前及早检测出 ATA 对生产高品质产品至关重要。气候变化引起的干旱严重影响了农业,包括葡萄种植业,尤其是土壤较浅、可用水量(AWC)减少的葡萄园。本研究考察了用于酿制 SW 的基酒(BW)中 AWC 与 ATA 之间的关系。十个葡萄园被分为三个 AWC 类别(低、中、高)。在两个生长季中对水分胁迫水平进行了监测,以探究其对植物生产行为和 AAP 发展的影响。在第一个年份,干旱条件导致所有 AWC 等级的 BW 都可能受到 ATA 污染。这种影响因AWC而异,低AWC葡萄园承受的压力更大,生产出的BW的AAP水平更高,植被生产失衡。与此相反,下一季降雨量异常,导致一些BW可能受到ATA的影响,但AWC等级之间的AAP含量或植被生产平衡没有明显差异。总之,在低AWC土壤上生长的葡萄树,其BW出现问题的风险较高,尤其是在干旱的年份。
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引用次数: 0
Impact of fig maceration under various conditions on physicochemical and sensory attributes of wine vinegar: A comprehensive characterization study. 不同条件下无花果浸渍对葡萄酒醋理化和感官属性的影响:综合表征研究
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-29 DOI: 10.1111/1750-3841.17485
Maria Del Pilar Segura-Borrego, Pilar Ramírez, Rocío Ríos-Reina, Maria Lourdes Morales, Raquel María Callejón, Juan Manuel León Gutiérrez

In this work, wine vinegars macerated with figs under different conditions have been characterized for the first time. Samples were analyzed by solid phase microextraction and gas chromatography coupled to mass spectrometry, sensory and physicochemical analysis. The results revealed that fig maceration of wine vinegars influenced the physicochemical, sensory, and volatile profiles of the final product, which were mainly affected by the fig amount (10, 20 or 30%), the fig type (fresh or dry) and the base wine vinegar (young or aged) used, and to a lesser extent the way the fig was added (crushed or chopped). The maceration, regardless the conditions used, increased the fruity notes, overall taste and visual impression, the tonality, total polyphenols and sugar content, and the presence of some volatile compounds, mainly terpenes and ketones, such as (Z)-p-mentha-2,8-dien-1-ol, or β-damascenone. Therefore, maceration increased the organoleptic complexity, which could enhance the acceptability of consumers towards these products. PRACTICAL APPLICATION: This research studied how wine vinegars macerated with figs affect in different ways their taste, smell, and chemical properties. Thus, the results showed that these wine vinegars made with fig maceration by using different amounts and types of figs, as well as the type of vinegar used, changed their characteristics, making them smell and taste fruitier and more pleasant. This could be useful for making better-tasting and more appealing vinegars for consumers who enjoy unique and enhanced flavors in their food.

在这项研究中,首次对在不同条件下浸渍无花果的葡萄酒醋进行了表征。通过固相微萃取、气相色谱-质谱联用、感官和理化分析对样品进行了分析。结果显示,无花果浸渍葡萄酒醋会影响最终产品的理化、感官和挥发性特征,主要受无花果量(10%、20% 或 30%)、无花果类型(新鲜或干果)和所使用的基酒醋(年轻或陈酿)的影响,其次是无花果的添加方式(压碎或切碎)。无论使用何种条件,浸渍都会增加果香、整体口感和视觉印象、色调、总多酚和糖分含量,以及一些挥发性化合物的存在,主要是萜烯和酮类,如 (Z)-p-mentha-2,8-dien-1-ol 或 β-大马士革酮。因此,浸渍增加了感官的复杂性,可以提高消费者对这些产品的接受度。实际应用:这项研究探讨了用无花果浸渍的葡萄酒醋如何以不同的方式影响其口感、气味和化学特性。因此,研究结果表明,通过使用不同数量和种类的无花果,以及使用不同种类的醋,用无花果浸渍制成的葡萄酒醋改变了它们的特性,使它们闻起来和尝起来更有果香,更令人愉悦。这对酿造口感更好、更吸引人的葡萄酒醋很有帮助,因为消费者喜欢在食物中添加独特的风味。
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引用次数: 0
A review of taste-active compounds in meat: Identification, influencing factors, and taste transduction mechanism. 肉类中的味觉活性化合物综述:识别、影响因素和味觉传导机制。
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-29 DOI: 10.1111/1750-3841.17480
Zerui You, Yilin Bai, Dongdong Bo, Yuqing Feng, Jiameng Shen, Yuanyuan Wang, Jing Li, Yueyu Bai

Poultry and livestock meat are important parts of the human diet. As living standards have improved, food taste has become a major influence on consumer quality assessment and meat purchasing choices. There is increasing research interest in meat taste and meat taste-active compounds, which include free amino acids, flavor nucleotides, taste-active peptides, organic acids, soluble sugars, and inorganic ions. Taste component research is also an important part of sensory science. A deeper understanding of the meat taste perception mechanism and interactions among different taste compounds will promote the development of meat science and sensory evaluation. This article reviews the main taste compounds in meat, factors influencing their concentrations, and the identification and measurement of taste-active compounds, as well as summarizing the mechanisms of taste sensing and perception. Finally, the future of scientific taste component evaluation is discussed. This review provides a theoretical basis for research on meat taste and an important reference for the development of the meat industry.

禽肉和畜肉是人类饮食的重要组成部分。随着生活水平的提高,食品口味已成为影响消费者质量评价和肉类购买选择的一个重要因素。人们对肉类味道和肉类味道活性化合物的研究兴趣日益浓厚,这些化合物包括游离氨基酸、味道核苷酸、味道活性肽、有机酸、可溶性糖和无机离子。味觉成分研究也是感官科学的重要组成部分。深入了解肉类味觉感知机制和不同味觉化合物之间的相互作用,将促进肉类科学和感官评价的发展。本文综述了肉类中的主要味觉化合物、影响其浓度的因素、味觉活性化合物的鉴定和测量,并总结了味觉感知机制。最后,还讨论了科学味觉成分评价的前景。这篇综述为肉类口味研究提供了理论依据,也为肉类工业的发展提供了重要参考。
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引用次数: 0
Measuring palatability of pet food products: Sensory components, evaluations, challenges, and opportunities. 测量宠物食品的适口性:感官成分、评估、挑战和机遇。
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-29 DOI: 10.1111/1750-3841.17511
Natalia Calderón, Brittany L White, Han-Seok Seo

The pet food industry is a growing business launching a variety of new products in the market. The acceptability or preference of pet food samples has traditionally been measured using either one-bowl or two-bowl tests. Academic researchers and professionals in the pet food industry have explored other methods, including the cognitive palatability assessment protocols and the ranking test, to evaluate more than two samples. A variety of approaches and perspectives were also utilized to predict palatability and key sensory attributes of pet foods, including descriptive sensory analysis by human-trained panelists and pet food caregivers' perceptions of pet food. This review article examined a range of testing methods for evaluating the palatability of pet foods, specifically targeting products for dogs and/or cats. It outlined the advantages and disadvantages of each method. Additionally, the review provided in-depth insights into the key sensory attributes of pet foods and the methodologies for assessing palatability. It also explored pets' behavioral responses and facial expressions in relation to different pet foods. Furthermore, this review discussed current challenges and future opportunities in pet food development, including the use of instrumental analyses and artificial intelligence-based approaches.

宠物食品行业是一个不断发展的行业,市场上不断推出各种新产品。宠物食品样本的可接受性或偏好性传统上是通过一碗或两碗测试来衡量的。学术研究人员和宠物食品行业的专业人士已经探索了其他方法,包括认知适口性评估协议和排序测试,以评估两个以上的样品。此外,还采用了多种方法和视角来预测宠物食品的适口性和关键感官属性,包括由经过人类培训的小组成员进行的描述性感官分析和宠物食品护理人员对宠物食品的感知。这篇综述文章研究了一系列评估宠物食品适口性的测试方法,特别是针对狗和/或猫的产品。文章概述了每种方法的优缺点。此外,文章还深入探讨了宠物食品的主要感官属性和适口性评估方法。综述还探讨了宠物对不同宠物食品的行为反应和面部表情。此外,本综述还讨论了宠物食品开发目前面临的挑战和未来的机遇,包括使用仪器分析和基于人工智能的方法。
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引用次数: 0
Effect of NaCl replacement by other salt mixtures on myofibrillar proteins: Underlining protein structure, gel formation, and chewing properties. 用其他盐混合物替代 NaCl 对肌纤维蛋白质的影响:强调蛋白质结构、凝胶形成和咀嚼特性。
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-29 DOI: 10.1111/1750-3841.17503
Haibo Shi, Yongjie Li, Jiabao Zheng, Xianqi Yao, Wei Wang, Igor Tomasevic, Weizheng Sun

The protein structure, gel changes, and chewing properties of low-sodium myofibrillar protein (MP) prepared by compound chloride salts (KCl/MgCl2, KCl/CaCl2, and KCl/MgCl2/CaCl2) and different substitution degrees (10%, 25%, and 40%) at same ionic strength (0.6 M) were investigated. The results revealed that the low-sodium MP gels containing CaCl2 manifested more liquid loss and less moisture content accompanied by obvious morphological shrinkage, while KCl/MgCl2 contributed to the gel juiciness. At high substitution degree of 40%, KCl/CaCl2 substitution rendered the gel with dense structure and highest strength, but worse water retention capacity. Using other compound chloride salts influenced the chewing efficiency, and CaCl2 substitution made the gel relatively hard to chew. The inhomogeneous structure accompanied by cluster blocks in KCl/CaCl2-substituted MP gel accelerated the overall fracture rate. During heating process, more proteins in CaCl2-substituted MP did not participate in gel formation, intervening the final gel properties. The chloride salt mixtures containing MgCl2, rather than CaCl2, avoided or alleviated the liquid loss and shrinkage of low-sodium MP gel within the substitution degree of 10%-40%, and substitution degree not exceeding 25% was more reasonable for the controlled qualities.

研究了在相同离子强度(0.6 M)下,复合氯盐(KCl/MgCl2、KCl/CaCl2和KCl/MgCl2/CaCl2)和不同取代度(10%、25%和40%)制备的低钠肌纤蛋白(MP)的蛋白质结构、凝胶变化和咀嚼特性。结果表明,含 CaCl2 的低钠 MP 凝胶的液体流失较多,水分含量较低,并伴有明显的形态收缩,而 KCl/MgCl2 则增加了凝胶的多汁性。在 40% 的高取代度下,KCl/CaCl2 取代后的凝胶结构致密,强度最高,但保水能力较差。使用其他复合氯盐会影响咀嚼效率,CaCl2的替代会使凝胶相对难以咀嚼。在 KCl/CaCl2 取代的 MP 凝胶中,非均质结构伴随着团块,加快了整体断裂率。在加热过程中,CaCl2 取代的 MP 中更多的蛋白质没有参与凝胶的形成,从而影响了凝胶的最终特性。含 MgCl2 而非 CaCl2 的氯盐混合物在 10%-40%的取代度范围内可避免或减轻低钠 MP 凝胶的液体流失和收缩,而不超过 25%的取代度对于控制质量更为合理。
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引用次数: 0
Brassica rapa L. (Tibetan Turnip) polysaccharide improves the immune function and regulates intestinal microbiota in immunosuppressive mice. 西藏萝卜多糖能改善免疫抑制小鼠的免疫功能并调节肠道微生物群。
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-25 DOI: 10.1111/1750-3841.17419
Yan Zhang, Qirui Xu, Yong Liu, Ying Liu, Jie Luo, Jia Liu, Siyu Yu

In this paper, active polysaccharides were extracted from Brassica rapa L. polysaccharide (BRP), and structural characterization was preliminarily investigated. Its immunomodulatory activity and molecular biological mechanisms in cyclophosphamide-induced immunosuppressed mice were also explored, as well as its effects on intestinal microbiota. Results indicate that BRP is an acidic heteropolysaccharide with the main components of Ara, GalA, and GlcA and has α- and β-glycosidic linkages with pyranose bonds. The results of the study showed that BRP could effectively improve the thymus and spleen indices and repair Cy-induced immune tissue damage in immunosuppressed mice. Meanwhile, BRP increased the immune cell activity and antioxidant levels in mice. In addition, BRP increased the secretion of cytokines (IL-1β, IL-6, IL-10, and TNF-α) and immunoglobulins (IgA, IgG) in mouse serum. It also regulates the relative expression of genes related to the TLR4/NF-κB signaling pathways as well as regulates the diversity and composition of mouse intestinal microbiota. In conclusion, BRP was able to regulated the immune function in immunosuppressed mice, providing a theoretical basis for the development of immunomodulators.

本文从芥蓝多糖(Brassica rapa L. polysaccharide,BRP)中提取了活性多糖,并对其结构特征进行了初步研究。同时还探讨了其在环磷酰胺诱导的免疫抑制小鼠体内的免疫调节活性和分子生物学机制,以及对肠道微生物群的影响。结果表明,BRP是一种酸性杂多糖,主要成分为Ara、GalA和GlcA,并具有以吡喃糖键连接的α-和β-糖苷键。研究结果表明,BRP 能有效改善免疫抑制小鼠的胸腺和脾指数,修复 Cy 诱导的免疫组织损伤。同时,BRP 还能提高小鼠免疫细胞的活性和抗氧化水平。此外,BRP 还能增加小鼠血清中细胞因子(IL-1β、IL-6、IL-10 和 TNF-α)和免疫球蛋白(IgA、IgG)的分泌。它还能调节 TLR4/NF-κB 信号通路相关基因的相对表达,以及调节小鼠肠道微生物群的多样性和组成。总之,BRP 能够调节免疫抑制小鼠的免疫功能,为开发免疫调节剂提供了理论依据。
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引用次数: 0
Comparative study on the difference of saponins and nutrients in Panax notoginseng powder dried by new rapid drying technique and traditional hot air. 新型快速干燥技术与传统热风干燥三七粉中皂甙和营养成分差异的比较研究
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-25 DOI: 10.1111/1750-3841.17424
Jing Zeng, YiFan Cheng, RuiQi Pu, YiFei Ning, Min Liu, Ji Ma, Xiu Ming Cui

Panax notoginseng (Burk.) F. H. Chen (PN), commonly known as PN, is a nutritious natural food with a long history of consumption and has traditionally been used for dietary purposes in the form of dried processed products. Currently, developed a dry processing at short time and room temperature (DRST), which is characterized by high efficiency and low cost. However, there are few studies on the impact of DSRT. In this study, the effects of conventional hot air drying (DHA) and the innovative drying technology DSRT on the key components of PN were evaluated for the first time. The results showed that DRST could obtain processed PN products with smaller particle sizes and that DRST-treated PN could increase the content of five saponins by 1.38% for Ginsenoside Rg1, 0.1% for Ginsenoside Re, 0.83% for Ginsenoside Rb1, 0.16% for Ginsenoside Rd, and 0.36% for PN saponin R1, relative to the content of five saponins that could be increased by conventional DHA. The metabolome results yielded a total of 1401 metabolites identified and analyzed, and 201 metabolites showed significant differences between the two techniques, which were expressed as amino acids, flavonoids, and other nutrient-active components. The results of this study indicate that the PN products produced by DRST technology have higher nutritional quality compared to traditional processing. This study provides support in the processing of PN and the development of PN products.

三七(Panax notoginseng (Burk.) F. H. Chen),俗称 PN,是一种营养丰富的天然食品,食用历史悠久,传统上一直以干燥加工产品的形式用于饮食目的。目前,已开发出一种短时间室温干燥加工方法(DRST),其特点是效率高、成本低。然而,有关短时室温干燥加工的影响的研究却很少。本研究首次评估了传统热风干燥(DHA)和创新干燥技术 DSRT 对 PN 关键成分的影响。结果表明,DRST 可获得粒径更小的 PN 加工产品,相对于传统 DHA 可增加的五种皂苷含量,DRST 处理的 PN 可使人参皂苷 Rg1 增加 1.38%,人参皂苷 Re 增加 0.1%,人参皂苷 Rb1 增加 0.83%,人参皂苷 Rd 增加 0.16%,PN 皂苷 R1 增加 0.36%。代谢组结果共鉴定和分析了 1401 种代谢物,其中 201 种代谢物在两种技术之间存在显著差异,表现为氨基酸、黄酮类化合物和其他营养活性成分。研究结果表明,与传统加工方法相比,DRST 技术生产的 PN 产品具有更高的营养质量。这项研究为加工 PN 和开发 PN 产品提供了支持。
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引用次数: 0
UPLC/HR-ESI-MS/MS and GC/MS profiling of Eriobotrya japonica L. fruit in correlation to its antioxidant, anti-inflammatory, and anti-arthritic effects. 用 UPLC/HR-ESI-MS/MS 和 GC/MS 分析枇杷果实的抗氧化、抗炎和抗关节炎作用。
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-25 DOI: 10.1111/1750-3841.17468
Dina Atef Waly, Aisha Hussein Abou Zeid, Reda Sayed Mohammed, Sherifa Fahmy Moustafa, Ali Mahmoud El-Halawany, Kawkab A Ahmed, Amany Ameen Sleem, El-Sayeda Ahmed El-Kashoury

Eriobotrya japonica Lindl. (Loquat) fruit is a subtropical edible fruit originally from China. It grows well in Egypt, but it is not widely known. In the current study, the fruit was extracted with 80% ethanol to get the total ethanol extract (TEE). A part of which was fractionated by dichloromethane to yield polar and nonpolar fractions (PF and NPF). The antioxidant and anti-inflammatory activities of the TEE were in vitro evaluated. The complete Freund's adjuvant (CFA) arthritis model was used to explore the in vivo biological assessment of the anti-arthritic properties in vivo of the TEE, PF, and NPF of the fruit. Additionally, the inspected limbs detached from all animals were subjected to histological inspection. Moreover, GC/MS analysis of the unsaponifiable (USF) and saponifiable (SF) fractions of the NPF was performed. Furthermore, 64 metabolites from various chemical classes were identified using UHPLC/HR-MS/MS analysis of the TEE of the fruit in both positive and negative ionization modes. The positive ionization mode of loquat fruit allowed for the first time the detection of two kinds of lyso-glycerophospholipids (Lyso-GPLs): lyso-glycerophosphoethanolamines (Lyso-PtdEtn) and lyso-glycerophosphocholines (Lyso-PtdCho). The fruit extracts exhibited a notable in vivo anti-arthritic activity by decreasing paw thickness in the treated rats and adjusting the inflammatory mediators. The TEE showed the highest anti-arthritic activity, followed by the PF that showed an observed activity, while the NPF exhibited the lowest activity. Histopathological findings showed a marked improvement in the arthritic condition of the excised limbs. Thus, E. japonica fruit may be considered as a promising natural antioxidant and anti-arthritic agent.

枇杷(Eriobotrya japonica Lindl.它在埃及生长良好,但并不广为人知。在本研究中,用 80% 的乙醇提取果实,得到总乙醇提取物(TEE)。其中一部分经二氯甲烷分馏,得到极性和非极性馏分(PF 和 NPF)。对 TEE 的抗氧化和抗炎活性进行了体外评估。采用完全弗氏佐剂(CFA)关节炎模型对果实的 TEE、PF 和 NPF 的体内抗关节炎特性进行了体内生物学评估。此外,还对所有动物的肢体进行了组织学检查。此外,还对 NPF 的不皂化物(USF)和皂化物(SF)馏分进行了气相色谱/质谱分析。此外,还利用超高效液相色谱/高分辨率质谱/多质谱对果实的 TEE 进行了正离子和负离子分析,鉴定出 64 种不同化学类别的代谢物。在枇杷果实的正离子模式下,首次检测到了两种溶血甘油磷脂(Lyso-GPLs):溶血甘油磷乙醇胺(Lyso-PtdEtn)和溶血甘油磷胆碱(Lyso-PtdCho)。果实提取物具有显著的体内抗关节炎活性,能降低受试大鼠的爪厚度并调节炎症介质。TEE 表现出最高的抗关节炎活性,其次是 PF,表现出观察到的活性,而 NPF 表现出最低的活性。组织病理学结果表明,切除肢体的关节炎状况得到了明显改善。因此,E. japonica 果实可被视为一种很有前景的天然抗氧化剂和抗关节炎剂。
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引用次数: 0
Estimation of the soluble solid content of citrus based on the fractional-order derivative and optimal band combination algorithm. 基于分数阶导数和最优波段组合算法的柑橘可溶性固体含量估算。
IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-10-25 DOI: 10.1111/1750-3841.17427
Shiqing Dou, Yuanxiang Deng, Wenjie Zhang, Jichi Yan, Zhengmin Mei, Minglan Li

The soluble solid content (SSC) is a primary characteristic index for evaluating the internal quality of citrus fruits. The development of rapid and nondestructive SSC detection techniques can help address the current issues of postharvest quality grading in China's citrus industry. In this study, a total of 261 experimental samples, including 70 Murcott, 91 Clementine, and 100 Navel orange, were divided into prediction and validation sets in a 7:3 ratio. After obtaining the reflection spectra and SSCs, SNV-FOD (Standard Normal Variate-Fractional-Order Derivative) was used to process the spectra, and the optimal band combination algorithm was introduced to select SSC-sensitive bands. Then, the obtained optimal dual-band combination was input into eight regression models for comparison, and the best performing models stacked ensemble models was selected. Finally, the H-ELR (HyperOpt-optimized ensemble learning regression) model, optimized using a Bayesian function, was applied for the effective estimation of SSC for three common citrus varieties in Guangxi, Murcott, Clementine, and Navel oranges. The results show that (1) the SNV-FOD preprocessing method proposed in this study improved the correlation coefficient with the SSC from 0.546 to 0.836 compared to that of the original spectrum, (2) the optimal dual-band combination (969 and 1069 nm) constructed by integrating the differential index and 1.2-order derivative yielded the most accurate results (RPD = 2.13), and (3) the H-ELR model, based on HyperOpt optimization, achieved good estimated performance (RPD = 2.46). PRACTICAL APPLICATION: This research contributes to the development of practical SSC prediction instruments with excellent universality and ease of application.

可溶性固形物含量(SSC)是评价柑橘类水果内部品质的主要特征指标。开发快速、无损的 SSC 检测技术有助于解决目前中国柑橘产业采后质量分级中存在的问题。本研究将 70 个墨角、91 个克莱门特和 100 个脐橙共 261 个实验样品按 7:3 的比例分为预测集和验证集。在获得反射光谱和 SSCs 后,使用 SNV-FOD(标准正态变分阶差)对光谱进行处理,并引入最优波段组合算法来选择 SSC 敏感波段。然后,将获得的最佳双波段组合输入八个回归模型进行比较,选出性能最佳的模型堆叠集合模型。最后,应用贝叶斯函数优化的 H-ELR(HyperOpt-optimized ensemble learning regression)模型,对广西三个常见柑橘品种墨橘、克莱门特橘和脐橙的 SSC 进行了有效估计。结果表明:(1) 与原始光谱相比,本研究提出的 SNV-FOD 预处理方法将与 SSC 的相关系数从 0.546 提高到 0.836;(2) 通过积分微分指数和 1.2 阶导数构建的最佳双波段组合(969 和 1069 nm)得到了最准确的结果(RPD = 2.13);(3) 基于 HyperOpt 优化的 H-ELR 模型取得了良好的估计性能(RPD = 2.46)。实际应用:这项研究有助于开发实用的 SSC 预测工具,它具有良好的通用性和易用性。
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Journal of Food Science
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