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A Novel Strategy in Managing Spent Probiotic Culture Media: Metabolome Identified Functional Candidates Against Hyperuricemia 管理消耗的益生菌培养基的新策略:代谢组学鉴定的抗高尿酸血症功能候选物。
IF 3.4 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-02-02 DOI: 10.1111/1750-3841.70897
Hongyu Zhang, Da Wang, Xiangheng Cao, Baiyu Zheng, Ruiyang Qiu, Wenhui Xu, Koukou Duan, Shuping Wei, Mengge Zhao, Shichao Han

With the rapid expansion of the probiotics industry, the management of spent culture media has emerged as a pressing concern. However, it also represents a potential resource for functional food development. In this study, we investigated and screened anti-hyperuricemia (HUA) metabolites of Lactobacillus paracasei (LP) from spent media (META). Administration of META significantly reduced serum uric acid (UA) (SUA) levels (p < 0.05), improved kidney function, and alleviated inflammatory infiltration. Furthermore, META modulated UA metabolism by regulating key enzymes (xanthine oxidase [XOD] and adenosine deaminase [ADA]) and urate transporters (organic anion transporter 3 [OAT3], glucose transporter 9 [GLUT9], and urate transporter 1 [URAT1]), while mitigating HUA-induced inflammation through the Toll-like receptor 4 (TLR4)/nuclear factor kappa-B (NF-κB) pathway. To identify bioactive compounds, untargeted metabolomics based on UHPLC-QTOF-MS/MS detected 361 upregulated metabolites. Network pharmacology and molecular docking (MD) analyses further highlighted potential candidates, including 2-isopropylmaleate and ricinoleic acid methyl ester. Collectively, these findings suggest that probiotic culture media waste may serve as a promising natural source for the development of anti-HUA functional foods. Further studies are warranted to assess the bioavailability and efficacy of these metabolites both in vitro and in vivo.

随着益生菌产业的迅速发展,废培养基的管理已成为一个迫切需要关注的问题。然而,它也代表了功能食品开发的潜在资源。在这项研究中,我们从废培养基(META)中研究和筛选副干酪乳杆菌(LP)抗高尿酸血症(HUA)代谢物。META可显著降低血清尿酸(UA) (SUA)水平(p < 0.05),改善肾功能,减轻炎症浸润。此外,META通过调节关键酶(黄嘌呤氧化酶[XOD]和腺苷脱氨酶[ADA])和尿酸转运蛋白(有机阴离子转运蛋白3 [OAT3]、葡萄糖转运蛋白9 [GLUT9]和尿酸转运蛋白1 [URAT1])调节UA代谢,同时通过toll样受体4 (TLR4)/核因子κ b (NF-κB)途径减轻UA诱导的炎症。为了鉴定生物活性化合物,基于UHPLC-QTOF-MS/MS的非靶向代谢组学检测了361种上调代谢物。网络药理学和分子对接(MD)分析进一步突出了潜在的候选药物,包括2-异丙基马来酸酯和蓖麻油酸甲酯。综上所述,这些发现表明,益生菌培养基废弃物可能是开发抗hua功能食品的一个有希望的天然来源。需要进一步的研究来评估这些代谢物在体外和体内的生物利用度和功效。
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引用次数: 0
Explosion Puffing of Native Mexican Maize: Physical Variables and Quality Assessment 墨西哥本土玉米爆炸膨化:物理变量和质量评价。
IF 3.4 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-02-02 DOI: 10.1111/1750-3841.70898
Leticia García-Cruz, María Gricelda Vázquez-Carrillo, César del Ángel Hernández-Galeno, Pedro Antonio-López, Fernando López-Morales

Snack consumption has become increasingly prevalent in modern dietary habits, largely driven by convenience. Puffed kernels represent a type of snack produced through the explosion puffing method, in which starch expansion is induced by pressure differentials. This study aimed to evaluate the puffing potential of different native maize populations by the explosion puffing method, identify variables associated with high-quality puffed kernels, and determine the proximate composition and total soluble phenol content of both raw and puffed kernels. The flotation index ranged from 5% to 98%, and maize samples was classified by endosperm hardness as very hard, hard, intermediate, soft, or very soft. Despite the wide variability in vitreous endosperm percentage, all hardness categories exhibited had a high proportion of puffed kernels (> 86.7%) and expansion volume between 10.1 and 26.6 cm3 g−1. Puffed kernels showed low moisture content (3.0%–3.6%), a porous and crunchy texture, high compressive force, and a preserved nutrient composition. Furthermore, the total soluble phenol content was higher in puffed kernels compared to their raw counterparts. This study provides an initial approach to utilizing dent maize for puffed kernel production, and demonstrates the effectiveness of explosion puffing in processing maize varieties with diverse physical characteristics.

Practical Applications

In Mexico, there is a wide diversity of maize that varies in size, color, shape, and hardness. Limited research has been conducted on the production of maize-based puffed products. In this study, we found that the explosion puffing method allows us to work with maize in a wide range of hardness, from very hard to very soft, but the highest puffing quality was achieved with hard and very hard maize. In the food industry, the use of this processing method can be promoted to process maize with varying hardness levels and to develop healthier snack alternatives, since the puffed kernels retain the nutritional properties of the raw kernel, including proteins and carbohydrates, while increasing their phenolic content.

零食消费在现代饮食习惯中越来越普遍,很大程度上是为了方便。膨化kernel膨化kernel膨化kernel膨化kernel膨化kernel膨化kernel膨化kernel膨化kernel膨化kernel膨化kernel膨化kernel膨化kernel本研究旨在利用爆炸膨化法评价不同本土玉米群体的膨化潜力,确定与膨化高质量玉米粒相关的变量,并确定膨化玉米粒和生玉米粒的近似组成和总可溶性酚含量。浮选指标范围为5% ~ 98%,玉米样品按胚乳硬度分为极硬、硬、中等、软、极软。尽管玻璃体胚乳百分比差异很大,但所有硬度类别的膨化核比例都很高(> 86.7%),膨胀体积在10.1 ~ 26.6 cm3 g-1之间。膨化后的果仁含水率低(3.0% ~ 3.6%),多孔松脆,抗压力大,营养成分保存完好。此外,膨化籽粒的总可溶性酚含量高于未加工的籽粒。本研究为利用凹陷玉米生产膨化籽粒提供了初步途径,并证明了爆炸膨化处理不同物理特性玉米品种的有效性。实际应用:在墨西哥,玉米品种繁多,大小、颜色、形状和硬度各不相同。对玉米膨化产品的生产进行了有限的研究。在这项研究中,我们发现爆炸膨化方法允许我们处理硬度范围很广的玉米,从非常硬到非常软,但硬玉米和非常硬玉米的膨化质量最高。在食品工业中,可以推广使用这种加工方法来加工不同硬度的玉米,并开发更健康的零食替代品,因为膨化的玉米粒保留了生玉米粒的营养特性,包括蛋白质和碳水化合物,同时增加了它们的酚类含量。
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引用次数: 0
Skyrocketing Submissions Part 2 暴涨提交部分2。
IF 3.4 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-02-02 DOI: 10.1111/1750-3841.70905
Richard Hartel
<p>In 2025, we set another record for the number of submissions, close to 3800. The figure shows how our submissions have increased over the past 10 years, steadily increasing until the COVID dip, then skyrocketing over the past 3 years. How many manuscript submissions are enough? Maybe at some point, we should see this increase as no longer a good thing.</p><p>.</p><p>We're not the only journal in this situation; in fact, most journals are seeing a boom in submissions. We speculated last year that the solid reputation of <i>JFS</i> was behind this increase, with authors shunning the journals that promised faster, but less stringent, peer review. I'm not so sure. I think there are many other factors, not the least of which is the increasing pressure to publish papers, both in the United States and around the world.</p><p>Over the course of my career, I've known faculty members with radically divergent publication strategies. One would chunk everything down to the “smallest publishable increment” (SPI) and end up with over 800 publications in one's career. Another colleague argued that fewer were better, as long as they were “seminal” works. Certainly, high numbers attract attention—often speakers are introduced with a note to the number of publications, suggesting that more is better. On the other hand, it's difficult most of the time to assess if a work is groundbreaking or not, especially since most scientific advances are incremental. More on that topic next month.</p><p>Contributing to the number of submissions, some programs have a standard that a PhD candidate must have five publications to graduate. In my opinion, this often (although not always) leads to publication of the SPI, a good way of padding the resume for both the PI (principal investigator) and the student.</p><p>The pressure to publish can be even greater in other countries, especially those with growing scientific institutions. In China, for example, professors are required to publish numerous papers per year, with half of those in Q1 journals—that is, the top quartile of journals by Impact Factor in a given subject area. The specific numbers depend on the institution and the professor's rank. In some institutions, publication bonuses are awarded based on the number and quality (as determined by the journal's high IF) of publications, providing a financial impetus for churning out articles, whether original research or reviews. I'm sure similar factors apply to other countries with increasing submission numbers.</p><p>Since our rejection rate has remained about the same (hovering between 76% and 78%), the increase in submissions means we have a consequent increase in the number of manuscripts we publish. Last year, we exceeded our previous high, with over 800 articles.</p><p>While we still think of rising submissions as a positive indicator, it comes with a cost. Every submission goes through a sequence of automated and manual checks before it even gets to an Editor. At <i>JFS
在2025年,我们又创造了提交作品数量的记录,接近3800份。该图显示了我们的提交量在过去10年里的增长情况,在COVID下降之前稳步增长,然后在过去3年里飙升。多少份稿件才足够?也许在某种程度上,我们应该看到这种增长不再是一件好事。我们不是唯一一家遇到这种情况的杂志;事实上,大多数期刊都看到了投稿量的激增。去年我们推测,JFS的良好声誉是这一增长背后的原因,因为作者们避开了那些承诺更快但不那么严格的同行评审的期刊。我不太确定。我认为还有许多其他因素,其中最重要的是在美国和世界各地发表论文的压力越来越大。在我的职业生涯中,我认识一些教师,他们的出版策略截然不同。一个人会把所有的东西都分解成“最小的可发表增量”(SPI),最终在他的职业生涯中发表了800多篇文章。另一位同事认为,只要是“开创性”的作品,数量越少越好。当然,高数字会吸引人们的注意——在介绍演讲者时,通常会在发表文章的数量上加上一个注释,表明发表文章越多越好。另一方面,大多数时候很难评估一项工作是否具有开创性,特别是因为大多数科学进步都是渐进的。下个月将详细讨论这个话题。考虑到提交的论文数量,一些项目有一个标准,即博士候选人必须有五篇论文才能毕业。在我看来,这通常(虽然不总是)导致SPI的出版,这是PI(主要研究者)和学生填充简历的好方法。在其他国家,尤其是那些科研机构不断壮大的国家,发表论文的压力可能更大。例如,在中国,教授被要求每年发表大量论文,其中一半发表在Q1期刊上,即某一学科领域影响因子排名前四分之一的期刊。具体数字取决于学校和教授的级别。在一些机构中,发表奖金是根据发表的数量和质量(由期刊的高影响因子决定)来发放的,这为大量发表文章提供了经济动力,无论是原创研究还是评论。我相信类似的因素也适用于其他提交数量不断增加的国家。由于我们的退稿率保持不变(徘徊在76%到78%之间),投稿量的增加意味着我们发表的稿件数量也随之增加。去年,我们发表了800多篇文章,超过了之前的最高记录。虽然我们仍然认为提交量的增加是一个积极的指标,但这是有代价的。在提交到编辑器之前,每个提交都要经过一系列的自动和手动检查。在JFS,我们扫描的内容包括识别相似度索引、人类主题或动物作品的IRB编号、适当的格式(例如,参考文献、图表等)、潜在的冲突(例如,作者推荐的审稿人来自同一机构)、所有共同作者的有效电子邮件地址、重复提交等。但是,诸如工作质量和是否适合期刊范围之类的事情,则留给编辑们来评估。提交的手稿越多,各级工作人员和编辑需要做的工作就越多。投稿量的增加意味着近年来我们在各个层次增加了许多编辑。我们从5年前的10位科学编辑(其中一些与CRFSFS共享)到现在的14位专门用于JFS。同样,助理编辑(ae)的数量也有所增加,以适应提交量的增加。编辑委员会的一般成员也有所增加。这些增长给IFT带来了额外的成本。投稿的增加也意味着我们需要更多潜在的审稿人。我相信这就是,至少在一定程度上,为什么我们的ae越来越难以让评论者同意一项任务。我一次又一次地从ae那里听到,每篇稿子只有3篇可靠的评论,这常常令人沮丧。随着提交量的增加,AE的工作变得越来越难。如果数量的增加也意味着手稿质量的提高,那就太好了,但有时这似乎是成反比的。有了造纸厂和人工智能增强的写作,作者投稿变得越来越容易。这意味着我们的编辑必须处理越来越多的低质量手稿。因此,编辑们也表达了一种更大的绝望感,他们不得不花时间在那些科学薄弱或甚至不适合我们研究范围的手稿上。也许我们不应该因为年复一年有更多的作品提交而高兴。尊敬的Rich Hartel,威斯康星大学麦迪逊分校食品科学杂志教授,首席博士
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引用次数: 0
Morphology Engineering: Uniform Ganoderma lucidum Pellet Formation in Coconut Juice by Bidirectional Submerged Fermentation 形态工程:双向深层发酵在椰汁中形成均匀的灵芝颗粒。
IF 3.4 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-02-02 DOI: 10.1111/1750-3841.70892
Ang Ren, Ran He, Jieying Wang, Wei Yi, Yueqian Liu, Zhiwen Qi, Bangchao Zhong, Longyu Wang, Ailiang Jiang, Ran Tao, Qin He

ABSTRACT

Two different mycelia morphologies (clumps and pellets) of Ganoderma lucidum can be produced by bidirectional submerged fermentation (BSF) with coconut water. No mycelia growth was observed when coconut milk was used as the substrate. Ganoderma lucidum pellets contributed to higher yields of polysaccharides and triterpenes, as well as stronger antioxidant activity. This study investigated the regulation of key fermentation parameters on G. lucidum pellet formation. These parameters included inoculum size, aeration rate, substrate viscosity, and rotation speed. It also established prediction models for pellet number and size. With increasing inoculum size, aeration rate, and rotation speed, pellet quantity increased while pellet size decreased. The opposite trend occurred with increasing substrate viscosity. Among these parameters, rotation speed exerted the most significant effect on both morphology regulation and pellet formation. The addition of coconut meat resolved the issue of no mycelia formation (including pellets) in coconut milk. It also enabled uniform pellet production in both coconut milk and coconut water. When coconut meat was added to coconut milk, the average pellet diameter was 3.5 mm, and the average pellet number was 3.34 × 105 pellets/L. This study contributes to the development of a novel functional food research field. It provides a potential method for producing uniform mycelia pellets.

利用椰子水双向深层发酵,可以生产出两种不同形态的灵芝菌丝(团状和球状)。以椰浆为底物时,菌丝未生长。灵芝微丸的多糖和三萜产量较高,抗氧化活性也较强。本试验研究了关键发酵参数对灵芝球团形成的调控。这些参数包括接种量、曝气率、基质粘度和转速。建立了颗粒数和颗粒大小的预测模型。随着接种量、曝气量和转速的增加,球团数量增加,球团尺寸减小。随着基材粘度的增加,情况正好相反。其中,转速对颗粒形态调控和成球的影响最为显著。椰肉的加入解决了椰奶中没有菌丝体形成(包括颗粒)的问题。它还使在椰奶和椰子水中均匀生产颗粒成为可能。椰肉加入椰奶时,平均颗粒直径为3.5 mm,平均颗粒数为3.34 × 105粒/L。本研究有助于开辟一个新的功能食品研究领域。为生产均匀菌丝球提供了一种可行的方法。
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引用次数: 0
Effects of Edible Fungi and Medicine-Food Homology Ingredients on Sensory Attributes and Emotional Responses to Chicken Soup. 食用菌和药食同源成分对鸡汤感官属性和情绪反应的影响。
IF 3.4 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-02-01 DOI: 10.1111/1750-3841.70900
Menglin Cheng, Binyu Wang, Huayi Suo, Zhen Wang, Haiwei Wang, Hongwei Wang

This study investigated the impact of two types of ingredients, edible fungi (Lentinula edodes, Agrocybe aegerita, Agaricus blazei Murill, and Dictyophora indusiate) and medicine and food homology (MFH) substances (Panax ginseng, Angelica sinensis, and Astragalus membranaceus) on the sensory attributes and emotional responses elicited by chicken soups. Eight chicken soup samples were evaluated by 12 trained panelists using descriptive analysis (DA) and by 104 consumers using the check-all-that-apply (CATA) method. The results showed that, in addition to significant differences in characteristic ingredient flavors, the intensity of other sensory attributes also differed significantly among the samples. Soups containing the three Agaricales fungi (Lentinula edodes, Agrocybe aegerita, and Agaricus blazei Murill) were more strongly associated with fullness, umami, and saltiness. In contrast, the soup prepared with Phallales (Dictyophora indusiate) exhibited a distinct flavor profile compared to the other edible fungi. Soups with MFH ingredients were more frequently associated with sweet or bitter flavors. While the overall trends between DA and CATA were consistent, many consumers were unable to accurately identify the characteristic flavors of the added ingredients. CATA emotional response data showed significant differences among the 29 emotional attributes. Among the samples, the soup containing Lentinula edodes received the highest consumer acceptance and was associated with positive emotions such as eagerness, pleasantness, and joy. In conclusion, the addition of different ingredients significantly affects the sensory and emotional attributes of chicken soup. These findings offer new ideas for chicken soup product development and consumer insights. PRACTICAL APPLICATIONS: This study formulates a variety of edible fungi or medicine and food homology (MFH) chicken soup combinations to enhance consumer choice and increase sales potential. Furthermore, it serves as a channel to convey consumer feedback to chefs and producers, enabling them to tailor product development to market preferences and create more consumer-led offerings.

本研究考察了两类食用菌成分(香菇、绿草、姬松茸和工业双孢菌)和药食同源物质(人参、当归和黄芪)对鸡汤感官属性和情绪反应的影响。8份鸡汤样品由12名训练有素的小组成员使用描述性分析(DA)和104名消费者使用检查所有适用(CATA)方法进行评估。结果表明,除了特征成分风味存在显著差异外,其他感官属性的强度在样品之间也存在显著差异。含有三种蘑菇属真菌(香菇、绿草菇和姬松茸)的汤与饱足感、鲜味和咸味的关系更密切。相比之下,与其他食用菌相比,用Phallales (Dictyophora industria)制备的汤表现出明显的风味特征。含有MFH成分的汤通常与甜味或苦味有关。虽然DA和CATA之间的总体趋势是一致的,但许多消费者无法准确识别添加成分的特征风味。CATA情绪反应数据显示29种情绪属性之间存在显著差异。在这些样品中,含有香菇的汤获得了最高的消费者接受度,并与积极的情绪联系在一起,如渴望、愉快和喜悦。综上所述,不同配料的添加对鸡汤的感官和情感属性有显著影响。这些发现为鸡汤产品开发和消费者洞察提供了新的思路。实际应用:本研究配制了多种食用菌或药物与食品同源(MFH)鸡汤组合,以增加消费者的选择,增加销售潜力。此外,它还可以作为向厨师和生产商传达消费者反馈的渠道,使他们能够根据市场偏好定制产品开发,并创造更多以消费者为主导的产品。
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引用次数: 0
Milk Kefir Beverage Improves Histomorphometry, Reduces Inflammatory Infiltrates and Desulfovibrio and Increases Lactobacillus in IL-10-/- Mice. 乳开菲尔饮料改善IL-10-/-小鼠组织形态学,减少炎症浸润和脱硫弧菌,增加乳酸菌。
IF 3.4 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-02-01 DOI: 10.1111/1750-3841.70879
Iasmim Xisto Campos, Vinicius Fernandes Paris, Mariana de Fátima Albuquerque Pereira, Gabriela de Cássia Ávila Alpino, Andressa Ladeira Bernardes, Larissa Gabriela Morais de Ávila, Marcella Duarte Villas Mishima, Manoela Maciel Dos Santos Dias, Soraya Torres Gaze Jangola, Tiago Antonio de Oliveira Mendes, Maria do Carmo Gouveia Peluzio

This study aimed to evaluate the effects of milk kefir on parameters related to inflammatory bowel disease (IBD) in interleukin-10 knockout (IL-10-/-) mice. Sixteen C57BL/6J IL-10-/- male mice were divided into two experimental groups. The control group (n = 8) received 0.4 mL of whole milk (UHT) and the kefir group (n = 8) received 0.4 mL of a fermented beverage made from milk kefir grains (2.4 × 108 colony-forming units [CFU]), both administered via gavage for 4 weeks. Feces were collected and body weight was measured weekly. At the end of the study, the animals were anesthetized and euthanized, and the small intestine and cecum content were collected for analysis. Proximate composition of kefir and microbiological analysis were conducted. Histomorphometry measurements and quantification of short-chain fatty acids (SCFAs) were conducted in small intestine. SFCAs were also evaluated in cecum content, and microbiota composition was evaluated in fecal samples. The kefir beverage improved histomorphometry characteristics of the small intestine, increased the number of goblet cells, reduced inflammatory infiltrates, increased Lactobacillus, and reduced Desulfovibrio, suggesting attenuation of the inflammatory process. Furthermore, the kefir beverage increased the concentration of butyrate, a short-chain fatty acid with anti-inflammatory properties, in the small intestine, which may also be associated with reduced inflammation in this group.

本研究旨在评估乳开菲尔对白细胞介素-10敲除(IL-10-/-)小鼠炎症性肠病(IBD)相关参数的影响。16只C57BL/6J IL-10-/-雄性小鼠分为两个实验组。对照组(n = 8)给予0.4 mL全脂牛奶(UHT),开菲尔组(n = 8)给予0.4 mL由牛奶开菲尔颗粒(2.4 × 108菌落形成单位[CFU])制成的发酵饮料,均通过灌胃给予4周。每周收集粪便并测量体重。在研究结束时,对动物进行麻醉和安乐死,并收集小肠和盲肠内容物进行分析。进行了克菲尔的近似组成和微生物学分析。在小肠中进行了短链脂肪酸(SCFAs)的组织形态学测量和定量。还评估了盲肠内容物中的SFCAs,并评估了粪便样品中的微生物群组成。开菲尔饮料改善了小肠的组织形态特征,增加了杯状细胞的数量,减少了炎症浸润,增加了乳酸杆菌,减少了Desulfovibrio,表明炎症过程的衰减。此外,开菲尔饮料增加了丁酸盐的浓度,丁酸盐是一种具有抗炎特性的短链脂肪酸,在小肠中,这也可能与减少炎症有关。
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引用次数: 0
Degradation Products of Guangdong Finger Citron Water-Soluble Polysaccharides by Gut Microbiota Ameliorate Type 2 Diabetes Mellitus via the Cyclic Adenosine Monophosphate Pathway. 肠道微生物降解产物广东指香橼水溶性多糖通过环磷酸腺苷途径改善2型糖尿病。
IF 3.4 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-02-01 DOI: 10.1111/1750-3841.70924
Yuxiao Wu, Cheng Zhong, Heming Liu, Zhiqin Wu, Aimei Zhou

Type 2 diabetes mellitus (T2DM) is a chronic metabolic disease defined by persistent hyperglycemia, primarily caused by insulin (INS) resistance and β-cell dysfunction. However, current pharmacological therapies are limited by adverse effects, highlighting the need for safe adjunctive strategies. Plant polysaccharides and their fermentation-derived degradation products exhibit multiple bioactivities and may be promising nutraceutical candidates. This study used degradation products of a water-soluble polysaccharide of finger citron from Guangdong Province (FCP-2-1) by gut micro biota fermentation (DFPG) as the research subject to investigate its hypoglycemic effects and underlying mechanisms in mice with T2DM. The findings indicated that degradation products of FCP-2-1 by gut micro biota fermentation after 8 h (DFPG-8) attenuated body weight loss, polydipsia, polyphagia, high fasting blood glucose, impaired oral glucose tolerance (OGT), and the elevated serum INS and glycated serum protein (GSP) in T2DM mice. In addition, DFPG-8 ameliorated lipid metabolism and attenuated pancreatic islet injury. Mechanistically, DFPG-8 activated colonic cyclic adenosine monophosphate (cAMP) /Protein Kinase A (PKA) and cAMP/Epac by cAMP signaling, up regulated cAMP response element-binding protein (CREB) and caudal type homeobox 2 (Cdx-2), enhanced glucagon gene (GCG) transcription, and promoted glucagon-like peptide-1 (GLP-1) synthesis. It also restored hypothalamic GLP-1 receptor (GLP-1R) expression, thereby modulating appetite and energy balance, reducing food intake, and increasing GLP-1 responsiveness, contributing to improved glycemic homeostasis. Collectively, these findings demonstrate that DFPG-8 may exert hypoglycemic effects by regulating the cAMP/PKA/Epac/GCG/GLP-1/GLP-1R signaling pathway, thereby improving glucose and lipid metabolism as well as appetite regulation, and support its potential development as a functional dietary supplement for the adjunctive management of T2DM.

2型糖尿病(T2DM)是一种慢性代谢疾病,主要由胰岛素(INS)抵抗和β细胞功能障碍引起,以持续高血糖为特征。然而,目前的药物治疗受到副作用的限制,强调需要安全的辅助策略。植物多糖及其发酵降解产物具有多种生物活性,可能是有前途的营养候选物质。本研究以广东指香橼水溶性多糖(FCP-2-1)经肠道菌群发酵(DFPG)降解产物为研究对象,探讨其对T2DM小鼠的降糖作用及其机制。结果表明,FCP-2-1经肠道微生物发酵8 h后的降解产物(DFPG-8)减轻了T2DM小鼠的体重减轻、多饮、多食、空腹血糖升高、口服糖耐量(OGT)降低、血清INS和糖化血清蛋白(GSP)升高。此外,DFPG-8改善脂质代谢,减轻胰岛损伤。机制上,dfg -8通过cAMP信号通路激活结肠环腺苷单磷酸(cAMP) /蛋白激酶A (PKA)和cAMP/Epac,上调cAMP应答元件结合蛋白(CREB)和尾型同源盒2 (Cdx-2),增强胰高血糖素基因(GCG)转录,促进胰高血糖素样肽-1 (GLP-1)合成。它还恢复下丘脑GLP-1受体(GLP-1R)的表达,从而调节食欲和能量平衡,减少食物摄入,增加GLP-1反应性,有助于改善血糖稳态。综上所述,这些研究结果表明,DFPG-8可能通过调节cAMP/PKA/Epac/GCG/GLP-1/GLP-1R信号通路发挥降糖作用,从而改善糖脂代谢和食欲调节,并支持其作为功能性膳食补充剂辅助治疗T2DM的潜力。
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引用次数: 0
Effect of Freeze-Drying and Spray-Drying on Emulsibility and Stability of Emulsions Stabilized by Covalent and Non-Covalent Whey Protein-Dextran Complexes. 冷冻干燥和喷雾干燥对共价和非共价乳清蛋白-葡聚糖络合物乳化性和稳定性的影响。
IF 3.4 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-02-01 DOI: 10.1111/1750-3841.70913
Zhen Li, Hongyu Cao, Yifei Wu, Hongbo Li, Guilian Sun, Kewen Li, Shuwen Zhang, Hongjuan Li, Jinghua Yu

This study focuses on the stability of whey protein isolate-dextran (WPI-D) complexes, exploring how their modification modes (covalent grafting/non-covalent mixing) and drying processes (spray-drying [SD]/freeze-drying [FD]) regulate structural properties and stability. Covalent WPI-D grafts (WPI-D-G) exhibit a higher degree of glycosylation, with significant fluorescence quenching and red shift. Fourier transform infrared (FTIR) spectral analysis reveals reduced absorbance of amide bands, collectively forming rigid cross-linked structures. Compared to non-covalent WPI-D mixtures (WPI-D-M), these structures exhibit superior emulsifying activity index (EAI), emulsion stability index (ESI), as well as stronger tolerance to environmental stresses (thermal stability: FD WPI-D-G (WPI-D-GF) maintain a particle size of 0.447 µm at 80°C, whereas SD WPI-D-G (WPI-D-GS) tend to aggregate; salt tolerance: stable at NaCl ≤ 20 mg/mL; pH stability: robust across pH 2-7). WPI-D-GF retain intact cross-linked structures, exhibiting enhanced emulsifying performance and colloidal stability (higher absolute zeta potential), whereas WPI-D-GS, despite forming smaller uniform spherical particles, undergo partial structural degradation. Regarding comparative stability under different conditions: WPI-D-GF outperforms WPI-D-GS in thermal stability, whereas covalent grafts from both drying methods demonstrate comparable salt and pH tolerance. These findings elucidate the structure-stability relationship of WPI-D complexes, thereby providing a theoretical foundation for regulating their stability through process optimization.

本研究主要关注乳清分离蛋白-葡聚糖(WPI-D)配合物的稳定性,探讨其修饰模式(共价接枝/非共价混合)和干燥工艺(喷雾干燥[SD]/冷冻干燥[FD])如何调节其结构特性和稳定性。共价WPI-D接枝(WPI-D- g)表现出更高程度的糖基化,具有明显的荧光猝灭和红移。傅里叶红外(FTIR)光谱分析显示酰胺带的吸光度降低,共同形成刚性交联结构。与非共价WPI-D混合物(WPI-D- m)相比,这些结构具有更好的乳化活性指数(EAI)和乳液稳定性指数(ESI),以及更强的环境应力耐磨性(热稳定性:FD WPI-D- g (WPI-D- gf)在80℃时保持0.447µm的粒径,而SD WPI-D- g (WPI-D- gs)倾向于聚集;耐盐性:在NaCl≤20mg /mL时稳定;pH稳定性:在pH 2-7范围内稳定)。WPI-D-GF保留了完整的交联结构,表现出更好的乳化性能和胶体稳定性(更高的绝对zeta电位),而WPI-D-GS尽管形成了更小的均匀球形颗粒,但会发生部分结构降解。关于不同条件下的相对稳定性:WPI-D-GF在热稳定性方面优于WPI-D-GS,而两种干燥方法的共价接枝具有相当的盐和pH耐受性。这些发现阐明了WPI-D配合物的结构-稳定性关系,为通过工艺优化调节其稳定性提供了理论基础。
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引用次数: 0
Assessment of Antioxidant Functions of Food Using Electron Paramagnetic Resonance Spectrometry: Verification Using Vinegar Samples. 用电子顺磁共振光谱法评价食品的抗氧化功能:用食醋样品验证。
IF 3.4 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-02-01 DOI: 10.1111/1750-3841.70902
Megumi Ueno, Minako Nyui, Ken-Ichiro Matsumoto, Ikuo Nakanishi, Hiroshi Abe, Hiroko P Indo, Akira Fujii, Masanobu Nagano, Jitbanjong Tangpong, Voravuth Somsak, Hideyuki J Majima

The abilities of food products to quench superoxide (O2 •-) and the hydroxyl radical (OH) and to directly reduce stable nitroxyl radicals were assessed in the present study using electron paramagnetic resonance (EPR)-based methods. The antioxidant functions of vinegar samples, including 1- and 2-year-fermented black vinegar, were also examined. The abilities of the vinegar products to quench O2 •- were in the order of 2-year-fermented black vinegar ≈ 1-year-fermented black vinegar > standard vinegar. Standard vinegar, 1-year-fermented black vinegar, and 2-year-fermented black vinegar did not reduce the stable nitroxyl radical. Apple-flavored black vinegar, containing 300 mg/100 g of ascorbic acid, directly reduced the stable nitroxyl radical. The OH-quenching ability of 2-year-fermented black vinegar was slightly stronger than that of 1-year-fermented and standard vinegars. OH-quenching ability appeared to be due to the sugars and/or proteins present in sample vinegars, particularly apple-flavored black vinegar, which had a high carbohydrate content. Adding vinegar to the reaction mixture enhanced the reduction of TEMPOL in the presence of GSH at a hyperthermal temperature. The enhancement of the reduction of TEMPOL was in the order of apple-flavored black vinegar >> 2-year-fermented black vinegar > 1-year-fermented black vinegar > standard vinegar. The reduction of TEMPOL was attributed to the quenching of H2O2, except for apple-flavored black vinegar, which directly reduced TEMPOL. Collectively, the present results suggest that fermentation increases the antioxidant ability of vinegar. The combination of EPR methods described herein is feasible for visualizing the antioxidant activities of food samples.

采用电子顺磁共振(EPR)技术,研究了食品对超氧化物(O2•-)和羟基自由基(•OH)的猝灭能力,以及对稳定的硝基自由基的直接还原能力。对发酵1年和2年的黑醋样品的抗氧化功能进行了研究。食醋产品对O2•-的猝灭能力依次为2年发酵黑醋≈1年发酵黑醋>标准醋。标准醋、发酵1年的黑醋和发酵2年的黑醋没有降低稳定的硝基自由基。苹果味黑醋,含有300毫克/100克抗坏血酸,直接还原稳定的硝基自由基。2年发酵黑醋的•oh猝灭能力略强于1年发酵和标准醋。•oh猝灭能力似乎是由于样品醋中存在的糖和/或蛋白质,特别是苹果味的黑醋,其碳水化合物含量很高。在GSH存在的高温条件下,向反应混合物中加入醋可以增强TEMPOL的还原。TEMPOL还原的增强程度依次为苹果味黑醋>> 2年发酵黑醋> 1年发酵黑醋>标准醋。除苹果味黑醋直接降低TEMPOL外,其他均归因于H2O2的猝灭作用。总之,目前的结果表明,发酵提高了醋的抗氧化能力。本文所述的EPR方法组合用于食品样品的抗氧化活性可视化是可行的。
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引用次数: 0
Ethnocentrism, Willing to Buy, and Perceived Price of Foods Originated From Specific Countries: An Empirical Study of Consumers in India. 民族中心主义、购买意愿与来自特定国家的食物感知价格:对印度消费者的实证研究。
IF 3.4 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2026-02-01 DOI: 10.1111/1750-3841.70919
Rajesh Kumar, Edgar I V Chambers, Ryan Kroll

This work investigates the degree of ethnocentrism among consumers in India to food imports from 13 different countries. Five food items commonly purchased in India were tested: bananas, milk, lentils, potatoes, and namkeen (a variety of salty, savory snacks). The 13 countries used as sources of origin were Australia, Brazil, China, Colombia, India, Kenya, Korea, New Zealand, Peru, Poland, Spain, Thailand, and United States. An online survey was conducted with N = 630 consumers with willingness to buy, and the price consumers were willing to pay asked for each individual food item from each country. Ethnocentric attitudes were assessed by consumers' responses to statements using the generalized ethnocentrism (GENE) scale. Exploratory factor analysis was used to identify key factors of ethnocentrism. Hierarchical and k-means cluster analysis approaches were used to segment consumers into high, moderate, and low ethnocentrism. Regardless of the varying degrees of ethnocentrism, Indian consumers exhibited a collective agreement to procure and allocate the highest premium for food products that are domestically produced. Developed nations, such as Australia, the United States, and New Zealand, were also regarded favorably, albeit as secondary to India. Consumers in India perceive China as the most unfavorable source for food imports among the 13 countries analyzed. Income level exerted the most pronounced influence on ethnocentric beliefs, followed by factors such as educational attainment, gender, and age demographics. Findings suggest a favorable dispositions towards indigenous food products, a clear ongoing transformation in the product choices of Indian consumers, and a notable predilection for local goods. PRACTICAL APPLICATIONS: This research aims to furnish food marketers with the necessary insights to develop a more effective strategic framework tailored for the Indian marketplace. This may serve as a mechanism to categorize consumer segments based on their levels of ethnocentrism, thereby facilitating the formulation of communication strategies centered on patriotic sentiments and the promotion of "Made in India" initiatives. Additionally, policymakers may leverage the findings of this study to reevaluate existing policies concerning imports and to enhance the domestic production of food commodities. This strategy may also be used in other countries to evaluate ethnocentrism in those markets.

这项工作调查了印度消费者对来自13个不同国家的食品进口的种族中心主义程度。在印度,我们测试了五种常见的食品:香蕉、牛奶、扁豆、土豆和namkeen(各种咸味小吃)。被用作原产地的13个国家是澳大利亚、巴西、中国、哥伦比亚、印度、肯尼亚、韩国、新西兰、秘鲁、波兰、西班牙、泰国和美国。对N = 630名愿意购买的消费者进行了在线调查,消费者愿意为每个国家的每种食品支付的价格。使用通用种族中心主义(GENE)量表,通过消费者对陈述的反应来评估种族中心主义态度。探索性因子分析用于确定种族中心主义的关键因素。使用层次和k-means聚类分析方法将消费者划分为高、中、低种族中心主义。不管不同程度的种族中心主义,印度消费者都表现出一种集体协议,即购买和分配国内生产的食品的最高溢价。发达国家,如澳大利亚、美国和新西兰,也被认为是有利的,尽管次于印度。在被分析的13个国家中,印度消费者认为中国是最不利的食品进口来源国。收入水平对种族中心主义信念的影响最为显著,其次是受教育程度、性别和年龄等因素。调查结果表明,对本土食品的有利倾向,印度消费者在产品选择上的明显持续转变,以及对当地商品的显著偏好。实际应用:本研究旨在为食品营销人员提供必要的见解,以制定更有效的战略框架,为印度市场量身定制。这可以作为一种机制,根据其种族中心主义的程度对消费者群体进行分类,从而促进以爱国情绪为中心的传播策略的制定和“印度制造”倡议的推广。此外,政策制定者可以利用这项研究的结果来重新评估有关进口的现有政策,并加强国内粮食商品的生产。这一战略也可用于其他国家,以评价这些市场中的种族中心主义。
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引用次数: 0
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Journal of Food Science
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