Pub Date : 2023-01-05DOI: 10.13057/nusbiosci/n150103
Novita Dewi Sambi, Y. Limbongan, A. M. Pata’dungan
Abstract. Sambi ND, Limbongan Y, Pata’dungan AM. 2023. Effect of dolomite and pig manure on growth and production of carrots (Daucus carota). Nusantara Bioscience 14: 22-29. This study aims to see how dolomite and pig manure affect carrot (Daucus carota L.) plant development and taproot production. The research was conducted from May to August 2021 in the Padangiring Village, Rantetayo Sub-district, Tana Toraja District, South Sulawesi, Indonesia. Three levels of dolomite in combination with four levels of pig manure were used: control (no treatment), and dolomite at 3 tons/ha, 6 tons/ha, and pig manure at control (no treatment), and 20 tons/ha, 30 tons/ha, and 40 tons/ha. The optimal dolomite level of 3 tons/ha significantly influenced plant height, the number of leaves, taproot length, and taproot diameter but had no significant effect on individual taproot weight, plot taproot weight, or taproot weight per hectare. The optimal pig manure dose of 4 kg/plot (40 tons/ha) showed a substantial influence on all investigated variables. The interaction between dolomite and pig manure substantially influenced taproot length but had no effect on the other observable variables. The optimum treatment combination was 3 tons/ha of dolomite with 40 tons/ha of pig manure, yielding 38.67 tons/ha of carrot taproots. Thus, commercial carrot growth and production may be boosted by a combination of dolomite and pig manure.
{"title":"Effect of dolomite and pig manure on growth and production of carrots (Daucus carota)","authors":"Novita Dewi Sambi, Y. Limbongan, A. M. Pata’dungan","doi":"10.13057/nusbiosci/n150103","DOIUrl":"https://doi.org/10.13057/nusbiosci/n150103","url":null,"abstract":"Abstract. Sambi ND, Limbongan Y, Pata’dungan AM. 2023. Effect of dolomite and pig manure on growth and production of carrots (Daucus carota). Nusantara Bioscience 14: 22-29. This study aims to see how dolomite and pig manure affect carrot (Daucus carota L.) plant development and taproot production. The research was conducted from May to August 2021 in the Padangiring Village, Rantetayo Sub-district, Tana Toraja District, South Sulawesi, Indonesia. Three levels of dolomite in combination with four levels of pig manure were used: control (no treatment), and dolomite at 3 tons/ha, 6 tons/ha, and pig manure at control (no treatment), and 20 tons/ha, 30 tons/ha, and 40 tons/ha. The optimal dolomite level of 3 tons/ha significantly influenced plant height, the number of leaves, taproot length, and taproot diameter but had no significant effect on individual taproot weight, plot taproot weight, or taproot weight per hectare. The optimal pig manure dose of 4 kg/plot (40 tons/ha) showed a substantial influence on all investigated variables. The interaction between dolomite and pig manure substantially influenced taproot length but had no effect on the other observable variables. The optimum treatment combination was 3 tons/ha of dolomite with 40 tons/ha of pig manure, yielding 38.67 tons/ha of carrot taproots. Thus, commercial carrot growth and production may be boosted by a combination of dolomite and pig manure.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2023-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49600760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-30DOI: 10.13057/nusbiosci/n140215
Emmanuel Ofudjaye Ndakara, O. Ohwo
Abstract. Ndakara OE, Ohwo O. 2022. The impacts of Hevea brasiliensis (rubber tree) plantation on soil nutrients in Southern Nigeria. Nusantara Bioscience 14: 234-239. This study investigated how Hevea brasiliensis (Willd. ex A.Juss.) Müll.Arg. impact on the soils within the humid rainforest ecosystem of Nigeria. The study used quasi-experimental and stratified random sampling techniques to select sampling units. Samples of soils under H. brasiliensis and rainforest (the control) were collected using an auger, and their laboratory analyses for total organic matter (TOM), total nitrogen (N), available phosphorus (P), exchangeable potassium (K), and pH were carried out using standard methods. Data generated were statistically analyzed using the mean, standard deviation, standard error of mean, and t-test. Findings showed that the soils under rainforest have higher nutrient properties than plantations of H. brasiliensis. Soil pH values were lower under rainforest than under plantations of H. brasiliensis. While TOM, N, and K differed significantly between rainforest and H. brasiliensis at a 5% confidence level, available phosphorus and pH were insignificant at a 5% confidence level. Tree species' capability to improve soil nutrition reflects its positive impact on the ecosystem. Since soil nutrients under H. brasiliensis are lower than soil nutrients under rainforest, efficient application of organic manure is required to improve the soil nutrient status for sustainable ecosystem functioning and management of the degraded rainforest environment.
摘要恩达卡拉OE, oho O. 2022。橡胶树种植对尼日利亚南部土壤养分的影响。生物科学学报14(2):234-239。本研究探讨了巴西橡胶树(Hevea brasiliensis, Willd.)A.Juss交货)。Mull.Arg。对尼日利亚潮湿雨林生态系统土壤的影响。本研究采用准实验和分层随机抽样技术选择抽样单位。采用螺旋钻采集巴西杉树和热带雨林(对照)土壤样品,采用标准方法进行土壤全有机质(TOM)、全氮(N)、有效磷(P)、交换性钾(K)和pH的室内分析。产生的数据采用均值、标准差、均值标准误差和t检验进行统计分析。结果表明,热带雨林土壤的养分特性优于巴西香人工林。热带雨林土壤pH值低于巴西木人工林土壤pH值。在5%的置信水平下,热带雨林和巴西杉树的TOM、N和K存在显著差异,而有效磷和pH在5%的置信水平下不显著。树种改善土壤营养的能力反映了其对生态系统的积极影响。由于巴西松土壤养分低于雨林土壤养分,因此需要有效施用有机肥来改善土壤养分状况,以实现退化雨林环境的可持续生态系统功能和管理。
{"title":"The impacts of Hevea brasiliensis (rubber tree) plantation on soil nutrients in Southern Nigeria","authors":"Emmanuel Ofudjaye Ndakara, O. Ohwo","doi":"10.13057/nusbiosci/n140215","DOIUrl":"https://doi.org/10.13057/nusbiosci/n140215","url":null,"abstract":"Abstract. Ndakara OE, Ohwo O. 2022. The impacts of Hevea brasiliensis (rubber tree) plantation on soil nutrients in Southern Nigeria. Nusantara Bioscience 14: 234-239. This study investigated how Hevea brasiliensis (Willd. ex A.Juss.) Müll.Arg. impact on the soils within the humid rainforest ecosystem of Nigeria. The study used quasi-experimental and stratified random sampling techniques to select sampling units. Samples of soils under H. brasiliensis and rainforest (the control) were collected using an auger, and their laboratory analyses for total organic matter (TOM), total nitrogen (N), available phosphorus (P), exchangeable potassium (K), and pH were carried out using standard methods. Data generated were statistically analyzed using the mean, standard deviation, standard error of mean, and t-test. Findings showed that the soils under rainforest have higher nutrient properties than plantations of H. brasiliensis. Soil pH values were lower under rainforest than under plantations of H. brasiliensis. While TOM, N, and K differed significantly between rainforest and H. brasiliensis at a 5% confidence level, available phosphorus and pH were insignificant at a 5% confidence level. Tree species' capability to improve soil nutrition reflects its positive impact on the ecosystem. Since soil nutrients under H. brasiliensis are lower than soil nutrients under rainforest, efficient application of organic manure is required to improve the soil nutrient status for sustainable ecosystem functioning and management of the degraded rainforest environment.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46476170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-30DOI: 10.13057/nusbiosci/n140216
Peni Ulfiani, A. M. Hasan, Y. Retnowati
Abstract. Ulfiani P, Hasan AM, Retnowati Y. 2022. Detection of Vibrio sp. on vaname shrimp (Litopanaeus vannamei) cultivation pond in Gorontalo, Indonesia. Nusantara Bioscience 14: 340-346. Vibrio is a pathogenic bacteria that can attack cultured shrimp. Therefore, it is necessary to increase the intensity of water changes and efforts to control water quality to maintain dissolved oxygen levels and manage organic matter content in ponds to prevent the increase of pathogenic bacteria, especially Vibrio. In addition, water physicochemical factors can influence the presence and population of Vibrio bacteria in ponds. This study aims to determine the presence of Vibrio sp. in vaname shrimp culture ponds in Gorontalo, Indonesia. Descriptive quantitative analysis was used to analyze the data collected from this study. Shrimp pond water samples were obtained from shrimp ponds in Molosipat Village, West Popayato Sub-district, Pohuwato District, Gorontalo, Indonesia, using a purposive sampling technique with an interval of three days from the first water change to the last before the water change in one period (approximately 10 days). The water samples were subjected to physicochemical analysis, including temperature, pH, salinity, ammonia, nitrate, and dissolved oxygen. The results showed that there were Vibrio bacteria in the Thiosulfate Citrate BileSalt Sucrose Agar (TCBSA) medium with a population that tends to increase in the range of 1.0 × 103 - 2.7 × 104 CFU/mL. The isolation results showed that two isolates were named ISL1 and ISL2, with the colony morphology characters of ISL1 isolates being green and ISL2 isolates being yellow. The results of microscopic observations of the two isolates showed comma-shaped and gram-negative cells. Molecular identification using the 16S rRNA gene and reconstruction of the phylogenetic tree using the Neighbor-Joining algorithm showed that ISL1 isolate is very closely related to Vibrio parahaemolyticus strain 11-1 (relationship level 99%) and ISL2 is closely related to V. parahaemolyticus strain B2-1 (relationship level 86%).
{"title":"Detection of Vibrio sp. on vaname shrimp (Litopanaeus vannamei) cultivation pond in Gorontalo, Indonesia","authors":"Peni Ulfiani, A. M. Hasan, Y. Retnowati","doi":"10.13057/nusbiosci/n140216","DOIUrl":"https://doi.org/10.13057/nusbiosci/n140216","url":null,"abstract":"Abstract. Ulfiani P, Hasan AM, Retnowati Y. 2022. Detection of Vibrio sp. on vaname shrimp (Litopanaeus vannamei) cultivation pond in Gorontalo, Indonesia. Nusantara Bioscience 14: 340-346. Vibrio is a pathogenic bacteria that can attack cultured shrimp. Therefore, it is necessary to increase the intensity of water changes and efforts to control water quality to maintain dissolved oxygen levels and manage organic matter content in ponds to prevent the increase of pathogenic bacteria, especially Vibrio. In addition, water physicochemical factors can influence the presence and population of Vibrio bacteria in ponds. This study aims to determine the presence of Vibrio sp. in vaname shrimp culture ponds in Gorontalo, Indonesia. Descriptive quantitative analysis was used to analyze the data collected from this study. Shrimp pond water samples were obtained from shrimp ponds in Molosipat Village, West Popayato Sub-district, Pohuwato District, Gorontalo, Indonesia, using a purposive sampling technique with an interval of three days from the first water change to the last before the water change in one period (approximately 10 days). The water samples were subjected to physicochemical analysis, including temperature, pH, salinity, ammonia, nitrate, and dissolved oxygen. The results showed that there were Vibrio bacteria in the Thiosulfate Citrate BileSalt Sucrose Agar (TCBSA) medium with a population that tends to increase in the range of 1.0 × 103 - 2.7 × 104 CFU/mL. The isolation results showed that two isolates were named ISL1 and ISL2, with the colony morphology characters of ISL1 isolates being green and ISL2 isolates being yellow. The results of microscopic observations of the two isolates showed comma-shaped and gram-negative cells. Molecular identification using the 16S rRNA gene and reconstruction of the phylogenetic tree using the Neighbor-Joining algorithm showed that ISL1 isolate is very closely related to Vibrio parahaemolyticus strain 11-1 (relationship level 99%) and ISL2 is closely related to V. parahaemolyticus strain B2-1 (relationship level 86%).","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44200501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-09DOI: 10.13057/nusbiosci/n140214
M. Abdulrahman
Abstract. Abdulrahman MD. 2022. Multivariate discrimination of selected taxa of the Fabaceae family. Nusantara Bioscience 15: 227-232. Nigeria is among the most interesting and diversified countries globally regarding tropical vegetation and medicinal plants. The taxonomy of the Fabaceae family is not entirely clear, to organize species into manageable groups that are helpful for taxonomical, conservational, or pharmacognostic study. This study aimed to discriminate the leaves of Dialium guineense Willd., Detarium microcarpum Guill. & Perr, Tamarindus indica L, Acacia nilotica (L.) Willd. ex Delile, Abrus precatorius L., Senna occidentalis (L.) Link, Erythrina senegalensis DC. and Pterocarpus erinaceus Poir. based on the mineral elements contents coupled with multivariate analysis. Three samples of each wild-growing species were collected. Unsupervised multivariate analysis using SIMCA-P (V.14.1, Umetrics Sweden) was employed. Five model groups were formed based on their mineral element contents. The species were fully discriminated along the PC1, accounting for 39.3% of the variation. Evidence from this study showed that a combination of mineral element analysis and chemometrics yielded a powerful classification method. Taxonomic identification of plants through biological research combined with chemometrics is an excellent method for preventing the adulteration or consumption of plants with excessive contents or harmful ingredients. However, a mix of molecular and developmental datasets is still necessary to explicitly examine their connections.
{"title":"Multivariate discrimination of selected taxa of the Fabaceae family","authors":"M. Abdulrahman","doi":"10.13057/nusbiosci/n140214","DOIUrl":"https://doi.org/10.13057/nusbiosci/n140214","url":null,"abstract":"Abstract. Abdulrahman MD. 2022. Multivariate discrimination of selected taxa of the Fabaceae family. Nusantara Bioscience 15: 227-232. Nigeria is among the most interesting and diversified countries globally regarding tropical vegetation and medicinal plants. The taxonomy of the Fabaceae family is not entirely clear, to organize species into manageable groups that are helpful for taxonomical, conservational, or pharmacognostic study. This study aimed to discriminate the leaves of Dialium guineense Willd., Detarium microcarpum Guill. & Perr, Tamarindus indica L, Acacia nilotica (L.) Willd. ex Delile, Abrus precatorius L., Senna occidentalis (L.) Link, Erythrina senegalensis DC. and Pterocarpus erinaceus Poir. based on the mineral elements contents coupled with multivariate analysis. Three samples of each wild-growing species were collected. Unsupervised multivariate analysis using SIMCA-P (V.14.1, Umetrics Sweden) was employed. Five model groups were formed based on their mineral element contents. The species were fully discriminated along the PC1, accounting for 39.3% of the variation. Evidence from this study showed that a combination of mineral element analysis and chemometrics yielded a powerful classification method. Taxonomic identification of plants through biological research combined with chemometrics is an excellent method for preventing the adulteration or consumption of plants with excessive contents or harmful ingredients. However, a mix of molecular and developmental datasets is still necessary to explicitly examine their connections.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2022-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43696068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-19DOI: 10.13057/nusbiosci/n140213
Mohamed R. Fouad, A. F. El-Aswad, M. Aly
Abstract. Fouad MR, El-Aswad AF, Aly MI. 2022. Acute toxicity, biochemical and histological of fenitrothion and thiobencarb on fish Nile tilapia (Oreochromis niloticus). Nusantara Bioscience 14: 217-226. The results show that the tested fenitrothion and thiobencarb are highly toxic to fish. However, fenitrothion is more toxic (1.6 times) on Oreochromis niloticus (Linnaeus, 1758) than thiobencarb. The determined 96-h LC50 values using a static bioassay system to Nile tilapia fingerlings (8-10 g) were 0.20 and 0.32 mg L-1 for fenitrothion and thiobencarb, respectively. The mortality rate of fish exposed to ½ 96-h LC50 of fenitrothion (0.10 mg/L) and thiobencarb (0.16 mg/L) for four days demonstrated was 20% mortality rate. Fish showed tremors, lethargy, decreased movement, and increased respiratory rhythm. The total activity of AChE in control was 5.61 ±0.03; it was significantly reduced to 4.92 ±0.03 in fenitrothion treatment and 1.13 ±0.02 in thiobencarb treatment. Fenitrothion decreased the specific activity from 0.83 ±0.01 for the control to 0.68 ±0.01, whereas thiobencarb reduced the specific activity to 0.22 ±0.01. Generally, thiobencarb inhibited AChE activity much more than fenitrothion; it produced 80% inhibition, while fenitrothion produced 12.5% inhibition. It showed a significant increase in liver GST and SOD activity of Nile tilapia exposed to the tested pesticides compared to the control. There were no histological alterations in the tissues of the control individuals. It was found that the herbicide thiobencarb affected the gills, kidneys, and liver of Nile tilapia more than the insecticide fenitrothion.
摘要Fouad MR, El-Aswad AF, Aly MI. 2022。杀虫磷和硫虫威对尼罗罗非鱼的急性毒性、生化和组织学研究。中国生物医学工程学报,14(2):517 - 526。结果表明,所测杀虫剂和硫虫威对鱼类有剧毒。然而,对尼罗ticus (Oreochromis niloticus, Linnaeus, 1758),虫硫磷的毒性是硫虫碳威的1.6倍。采用静态生物测定系统对尼罗罗非鱼鱼种(8 ~ 10 g)测定的96 h LC50值分别为0.20和0.32 mg L-1。氟虫硫磷(0.10 mg/L)和硫虫威(0.16 mg/L) 1 / 96 h LC50连续4天,死亡率为20%。鱼表现为震颤、嗜睡、运动减少和呼吸节奏加快。对照组乙酰胆碱酯酶总活性为5.61±0.03;非硝硫磷组为4.92±0.03,硫威组为1.13±0.02。比活性由对照组的0.83±0.01降至0.68±0.01,而噻虫威则降至0.22±0.01。一般来说,硫虫威对乙酰胆碱酯酶活性的抑制作用比氟硫磷大得多;它的抑制率为80%,而非硝硫磷的抑制率为12.5%。结果表明,与对照相比,接触杀虫剂的尼罗罗非鱼肝脏GST和SOD活性显著增加。对照个体的组织未见组织学改变。除草剂硫虫威对尼罗罗非鱼鳃、肾脏和肝脏的影响大于杀虫剂杀虫磷。
{"title":"Acute toxicity, biochemical and histological of fenitrothion and thiobencarb on fish Nile tilapia (Oreochromis niloticus)","authors":"Mohamed R. Fouad, A. F. El-Aswad, M. Aly","doi":"10.13057/nusbiosci/n140213","DOIUrl":"https://doi.org/10.13057/nusbiosci/n140213","url":null,"abstract":"Abstract. Fouad MR, El-Aswad AF, Aly MI. 2022. Acute toxicity, biochemical and histological of fenitrothion and thiobencarb on fish Nile tilapia (Oreochromis niloticus). Nusantara Bioscience 14: 217-226. The results show that the tested fenitrothion and thiobencarb are highly toxic to fish. However, fenitrothion is more toxic (1.6 times) on Oreochromis niloticus (Linnaeus, 1758) than thiobencarb. The determined 96-h LC50 values using a static bioassay system to Nile tilapia fingerlings (8-10 g) were 0.20 and 0.32 mg L-1 for fenitrothion and thiobencarb, respectively. The mortality rate of fish exposed to ½ 96-h LC50 of fenitrothion (0.10 mg/L) and thiobencarb (0.16 mg/L) for four days demonstrated was 20% mortality rate. Fish showed tremors, lethargy, decreased movement, and increased respiratory rhythm. The total activity of AChE in control was 5.61 ±0.03; it was significantly reduced to 4.92 ±0.03 in fenitrothion treatment and 1.13 ±0.02 in thiobencarb treatment. Fenitrothion decreased the specific activity from 0.83 ±0.01 for the control to 0.68 ±0.01, whereas thiobencarb reduced the specific activity to 0.22 ±0.01. Generally, thiobencarb inhibited AChE activity much more than fenitrothion; it produced 80% inhibition, while fenitrothion produced 12.5% inhibition. It showed a significant increase in liver GST and SOD activity of Nile tilapia exposed to the tested pesticides compared to the control. There were no histological alterations in the tissues of the control individuals. It was found that the herbicide thiobencarb affected the gills, kidneys, and liver of Nile tilapia more than the insecticide fenitrothion.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2022-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48007679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-09DOI: 10.13057/nusbiosci/n140211
Pawan Kumar, T. Thakur, D. Deepika, N. Sharma
Abstract. Kumar P, Thakur TS, Deepika, Sharma N. 2022. Diversity studies on insect pests of high altitudinal transitional zones of North-western Himalayas. Nusantara Bioscience 14: 203-210. Class Insecta constitute a major fauna and comprise many species of economic importance. Due to climate change and increase in temperature, many insect species are changing their habitat considerably and shifting their hosts, which leads to changes in the diversity of insect pests at different altitudinal gradients. High altitude forest cover is not large and massive as plains forest cover, but it supports some of the very important economical tree species like- Quercus sp., Himalayan Poplar, Betula sp., Abies pindrow (Royle ex D.Don) Royle, Juniper spp., Birdcherry, Maple, etc. The present study analyzed any change in insect pest incidences and diversity of pest species due to the change in host preferences or climatic patterns. The study was conducted at four selected sites viz. Rohtang area (Kullu Forest Division), Chanshal area (Rohru Forest Division), Sach area (Churah Forest Division), and Indrahar area (Dharamshala Forest Division) of high altitudinal transitional zones of Himachal Pradesh, India, to study insect pest diversity. A total of 32 insect species were recorded during the study period comprised of the insect orders viz., Coleoptera, Hymenoptera, Hemiptera, Orthoptera, Dermaptera, and Lepidoptera. The present investigation led to the finding that species of Coleoptera (beetles) were the most dominant insects attacking trees of the high altitudinal transitional zone, followed by Lepidoptera (Butterflies and moths) and Hemiptera (aphids).
{"title":"Diversity studies on insect pests of high altitudinal transitional zones of North-western Himalayas","authors":"Pawan Kumar, T. Thakur, D. Deepika, N. Sharma","doi":"10.13057/nusbiosci/n140211","DOIUrl":"https://doi.org/10.13057/nusbiosci/n140211","url":null,"abstract":"Abstract. Kumar P, Thakur TS, Deepika, Sharma N. 2022. Diversity studies on insect pests of high altitudinal transitional zones of North-western Himalayas. Nusantara Bioscience 14: 203-210. Class Insecta constitute a major fauna and comprise many species of economic importance. Due to climate change and increase in temperature, many insect species are changing their habitat considerably and shifting their hosts, which leads to changes in the diversity of insect pests at different altitudinal gradients. High altitude forest cover is not large and massive as plains forest cover, but it supports some of the very important economical tree species like- Quercus sp., Himalayan Poplar, Betula sp., Abies pindrow (Royle ex D.Don) Royle, Juniper spp., Birdcherry, Maple, etc. The present study analyzed any change in insect pest incidences and diversity of pest species due to the change in host preferences or climatic patterns. The study was conducted at four selected sites viz. Rohtang area (Kullu Forest Division), Chanshal area (Rohru Forest Division), Sach area (Churah Forest Division), and Indrahar area (Dharamshala Forest Division) of high altitudinal transitional zones of Himachal Pradesh, India, to study insect pest diversity. A total of 32 insect species were recorded during the study period comprised of the insect orders viz., Coleoptera, Hymenoptera, Hemiptera, Orthoptera, Dermaptera, and Lepidoptera. The present investigation led to the finding that species of Coleoptera (beetles) were the most dominant insects attacking trees of the high altitudinal transitional zone, followed by Lepidoptera (Butterflies and moths) and Hemiptera (aphids).","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2022-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49250768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-28DOI: 10.13057/nusbiosci/n140212
S. Hernosa, Sanggam Dera Rosa Tampubolon, L. Siregar
Abstract. Hernosa SP, Tampubolon SDR, Siregar LAM. 2022. The use of Indole Butyric Acid on the growth of dragon fruit plant stem cuttings. Nusantara Bioscience 14: 211-216. The difficulty of root growth of dragon fruit cuttings is affected by stem rot; one way to increase the growth of cuttings is to use a growth regulator, namely Indole Butyric Acid (IBA), which is used to stimulate the growth of dragon fruit rootstock cuttings. This research was conducted in Sidorukun Village, Pangkatan Sub-district, Labuhan Batu District, North Sumatra, Indonesia, at an altitude of ± 40 m above sea level, from June to October 2020. The research design used was a randomized block design with five treatments. The concentration of the IBA hormone consists of B0 : 0 ppm, B1: 4,500 ppm, B2: 5,500 ppm, B3: 6,500 ppm, B4: 7,500 ppm, and five replicates, so the entire experimental plot was 25. Parameters observed were percentage of shoot emergence, age of shoot emergence, shoot length (cm), shoot fresh weight (g), shoot dry weight (g), root length (cm), fresh root weight (g), root volume (g), and root dry weight (g). Data analysis used a linear model followed by Duncan's test. It is known that the administration of Indole butyric acid (IBA) significantly affected the observed parameters on shoot emergence, age of shoot emergence, number of shoots, root length, fresh root weight, root volume, and root dry weight. However, it did not affect the observed parameters, namely, shoot length, shoot fresh weight and shoot dry weight. On the other hand, B4 concentration (7,500 ppm) gave better shoot growth, root weight, and root dry weight.
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Pub Date : 2022-10-24DOI: 10.13057/nusbiosci/n140210
F. Asmelash, Emebet Getachew
Abstract. Asmelash F, Getachew E. 2022. Suitability of selected legume (Vachellia spp.) tree species for forest restoration in the Central Ethiopian highlands. Nusantara Bioscience 14: 195-202. This study aimed to evaluate the comparative suitability of the legume tree species viz., Vachellia abyssinica (Hochst. ex. Benth.) Kyal. & Boatwr., Vachellia etbaica (Schweinf.) Kyal. & Boatwr., Vachellia lahai (Steud. & Hochst. ex Benth.) Kyal. & Boatwr., and Vachellia seyal (Delile) P.J.H.Hurter for restoring forests in Central Ethiopian highlands. The suitability of three accessions of V. seyal was also compared. The correlation between root nodule number and root Arbuscular Mycorrhizal Fungi (AMF) colonization (RC) and seedlings’ growth variables were computed, and the effect of seedling age on nodule number and RC was determined. Seedlings were grown for nine months on degraded local soil in a mesh house in central Ethiopia. We measured shoot height, shoot fresh weight, rooting depth, root nodule number, and RC in the third and ninth months. The one-way ANOVA results indicated that tree species and/or accession (for V. seyal) had a significant (p<0.05) effect on all the measured variables except shoot height and rooting depth at the ninth month. Generally, nodule number and RC increased with seedling age. However, according to the independent t-test results, significant (p<0.05) differences were recorded for V. abyssinica, with a 57.16% reduction in nodule number, and V. seyal accession-1, with a 418.52% increase in RC. The Spearman’s rank correlation results indicated that the correlation between nodule number and RC was weak and non-significant (p>0.05) both in the third and ninth months. Based on the measured growth variables, nodule number (N-fixation potential), and RC, V. etbaica was the least suitable species for forest restoration in central Ethiopian highlands. The remaining species/accessions are comparably suitable. The V. abyssinica lost its comparative fitness with seedling age, maybe because it is a provenance far away from central Ethiopia. However, V. seyal accession-3, the furthest provenance, has performed much better. The legume trees of Ethiopia are less studied. Their role as environmental engineers could be better understood by knowing more about their root traits. Therefore, this study could motivate future research in this regard. Long-term experiments are required to consider more legume tree species and provenances in the future.
摘要陈晓明,陈晓明。埃塞俄比亚中部高原豆科植物(Vachellia spp.)树种恢复森林的适宜性。中国生物医学工程学报(英文版);本研究旨在评价豆科树种Vachellia abyssinica (Hochst.)的相对适宜性。Benth。)Kyal。& Boatwr。《经济学》(瑞士)Kyal。& Boatwr。,瓦切利亚·拉海(斯蒂德)。& Hochst。Benth交货)。Kyal。& Boatwr。和Vachellia seal (Delile) P.J.H.Hurter,以恢复埃塞俄比亚中部高地的森林。并比较了3个品种的适宜性。计算了根瘤数、根丛枝菌根真菌(AMF)定殖量(RC)与幼苗生长变量的相关性,确定了苗龄对根瘤数和RC的影响。在埃塞俄比亚中部的一个网状房屋里,幼苗在退化的当地土壤上生长了9个月。在第3个月和第9个月分别测定了茎高、茎鲜重、生根深度、根瘤数和RC。单因素方差分析结果表明,在第3个月和第9个月,树木种类和(或)增加(以梧桐为例)显著(p0.05)。基于测量的生长变量、根瘤数(固氮电位)和RC,埃塞黑是埃塞俄比亚中部高原最不适合森林恢复的树种。其余的物种/添加物比较适合。这可能是因为它是一个远离埃塞俄比亚中部的种源。然而,最远来源的V. seal -3的表现要好得多。埃塞俄比亚的豆科树木较少被研究。通过更多地了解他们的根本特征,可以更好地理解他们作为环境工程师的角色。因此,本研究可以激励未来在这方面的研究。未来需要进行长期的试验,以考虑更多的豆科树种和种源。
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Pub Date : 2022-10-14DOI: 10.13057/nusbiosci/n140209
Aurora Dyas Anasya, S. Sugiyarto, E. Mahajoeno
Abstract. Anasya AD, Sugiarto, Mahajoeno E. 2022. Development of semi-artificial feed in the larva stage of the black soldier fly Hermetia illucens (Diptera: Stratiomyidae). Nusantara Bioscience 14: 188-194. Alternative food sources in fish farming besides pelleted feed are generally used, but many have also used biotic materials, larvae of the black soldier fly (Hermetia illucens L.). Maggot stadia larvae have a high protein content of more than 19%, could be mass-produced, are low-priced, and has a fast growth time. Therefore, high levels of protein and nutrients in maggots can be increased through suitable semi-artificial formulations. The purpose of this study was to determine the nutritional value of the semi-artificial feed recipe given and to determine the effectiveness of the semi-artificial feed recipe in increasing the nutritional value and survival of larvae. Data analysis used qualitative analysis with descriptive analysis method and quantitative analysis by determining the amount of increased nutritional value of H. illucens. The research method was carried out in several stages, including (i) insect rearing obtained 2nd generation of tillers (F2); (ii) manufacture of semi-artificial feed recipes; (iii) calculation of insect survival; (iv) measurement of larval mass weight after treatment; (v) testing of nutritional content value includes water content, fat content and protein content of H. illucens after treatment. Results of the evaluation larval survival after treatment were effective enough 100%, larval instar life phase was between 28-32 days, while the highest increase in the nutritional value of larvae water content was 45.90%, fat content 7.25%, and protein content 45.95%, the average increase in mass weight of larvae was 12.50%.
{"title":"Development of semi-artificial feed in the larva stage of the black soldier fly Hermetia illucens (Diptera: Stratiomyidae)","authors":"Aurora Dyas Anasya, S. Sugiyarto, E. Mahajoeno","doi":"10.13057/nusbiosci/n140209","DOIUrl":"https://doi.org/10.13057/nusbiosci/n140209","url":null,"abstract":"Abstract. Anasya AD, Sugiarto, Mahajoeno E. 2022. Development of semi-artificial feed in the larva stage of the black soldier fly Hermetia illucens (Diptera: Stratiomyidae). Nusantara Bioscience 14: 188-194. Alternative food sources in fish farming besides pelleted feed are generally used, but many have also used biotic materials, larvae of the black soldier fly (Hermetia illucens L.). Maggot stadia larvae have a high protein content of more than 19%, could be mass-produced, are low-priced, and has a fast growth time. Therefore, high levels of protein and nutrients in maggots can be increased through suitable semi-artificial formulations. The purpose of this study was to determine the nutritional value of the semi-artificial feed recipe given and to determine the effectiveness of the semi-artificial feed recipe in increasing the nutritional value and survival of larvae. Data analysis used qualitative analysis with descriptive analysis method and quantitative analysis by determining the amount of increased nutritional value of H. illucens. The research method was carried out in several stages, including (i) insect rearing obtained 2nd generation of tillers (F2); (ii) manufacture of semi-artificial feed recipes; (iii) calculation of insect survival; (iv) measurement of larval mass weight after treatment; (v) testing of nutritional content value includes water content, fat content and protein content of H. illucens after treatment. Results of the evaluation larval survival after treatment were effective enough 100%, larval instar life phase was between 28-32 days, while the highest increase in the nutritional value of larvae water content was 45.90%, fat content 7.25%, and protein content 45.95%, the average increase in mass weight of larvae was 12.50%.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47125072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-10DOI: 10.13057/nusbiosci/n140208
Rahul Saikh, K. Murmu, Arup Sarkar, Ramyajit Mondal, K. Jana
Abstract. Saikh R, Murmu K, Sarkar A, Mondal R, Jana K. 2022. Effect of foliar zinc application on growth and yield of rice (Oryza sativa) in the Indo-Gangetic Plains of India. Nusantara Bioscience 14: 182-187. A field experiment was conducted on rice cv. Satabdi (IET-4768) to investigate the effect of foliar zinc application at different stages during the post-Kharif season of 2019. The field experiment was carried out at 'C' block farm of (B.C.K.V), Kalyani, India, with eight different foliar 0.5% 0.5% ZnSO4 (ZnSO4) are T1: Control (without foliar application), T2: Foliar application of 0.5% ZnSO4 at Panicle Initiation, T3: Foliar application of 0.5% ZnSO4 at Booting, T4: Foliar application of 0.5% ZnSO4 at Panicle Initiation and 1 week after flowering, T5: Foliar application of 0.5% ZnSO4 at Panicle at 1 week after Flowering, T6: Foliar application of 0.5% ZnSO4 at Panicle at 2 weeks after flowering, T7: Foliar application of 0.5% ZnSO4 at Panicle at 1 week and 2 weeks after flowering and T8: Foliar application of 0.5% ZnSO4 at Panicle Initiation, Booting, 1 week and 2 weeks after flowering respectively in randomized complete block design with three replication. The result of the experiment revealed that rice plants treated with the combination of T4i.e. Foliar application of 0.5% ZnSO4 at Panicle Initiation and 1 week after flowering have resulted in the highest grain yield of 5.09 t/ha, which was 50.59% higher (3.38 t/ha) than the T1i.e. the control. Furthermore, residual nutrient status was also highest in the plot treated with T4i.e. Foliar application of 0.5% ZnSO4 at Panicle Initiation and 1 week after flowering.
{"title":"Effect of foliar zinc application on growth and yield of rice (Oryza sativa) in the Indo-Gangetic Plains of India","authors":"Rahul Saikh, K. Murmu, Arup Sarkar, Ramyajit Mondal, K. Jana","doi":"10.13057/nusbiosci/n140208","DOIUrl":"https://doi.org/10.13057/nusbiosci/n140208","url":null,"abstract":"Abstract. Saikh R, Murmu K, Sarkar A, Mondal R, Jana K. 2022. Effect of foliar zinc application on growth and yield of rice (Oryza sativa) in the Indo-Gangetic Plains of India. Nusantara Bioscience 14: 182-187. A field experiment was conducted on rice cv. Satabdi (IET-4768) to investigate the effect of foliar zinc application at different stages during the post-Kharif season of 2019. The field experiment was carried out at 'C' block farm of (B.C.K.V), Kalyani, India, with eight different foliar 0.5% 0.5% ZnSO4 (ZnSO4) are T1: Control (without foliar application), T2: Foliar application of 0.5% ZnSO4 at Panicle Initiation, T3: Foliar application of 0.5% ZnSO4 at Booting, T4: Foliar application of 0.5% ZnSO4 at Panicle Initiation and 1 week after flowering, T5: Foliar application of 0.5% ZnSO4 at Panicle at 1 week after Flowering, T6: Foliar application of 0.5% ZnSO4 at Panicle at 2 weeks after flowering, T7: Foliar application of 0.5% ZnSO4 at Panicle at 1 week and 2 weeks after flowering and T8: Foliar application of 0.5% ZnSO4 at Panicle Initiation, Booting, 1 week and 2 weeks after flowering respectively in randomized complete block design with three replication. The result of the experiment revealed that rice plants treated with the combination of T4i.e. Foliar application of 0.5% ZnSO4 at Panicle Initiation and 1 week after flowering have resulted in the highest grain yield of 5.09 t/ha, which was 50.59% higher (3.38 t/ha) than the T1i.e. the control. Furthermore, residual nutrient status was also highest in the plot treated with T4i.e. Foliar application of 0.5% ZnSO4 at Panicle Initiation and 1 week after flowering.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2022-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47264306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}