Pub Date : 2022-02-15DOI: 10.13057/nusbiosci/n140102
M. Megersa, Samuel Woldetsadik
Abstract. Megersa M, Woldetsadik S. 2022. Ethnobotanical study of medicinal plants used by local communities of Damot Woyde District, Wolaita Zone, Southern Ethiopia. Nusantara Bioscience 14: 10-24. Humans have used traditional medicines mainly of plant origins to treat diseases. Early humans faced a tremendous challenge when searching for natural products used as medicines. This study reports on an ethnobotanical study that focused on the traditional medicinal plants used by local communities to treat human and livestock diseases. An ethnobotanical study on medicinal plants was conducted from February 2020 to October 2020. This involved semi-structured interviews, field observations, market surveys and group discussions with informants to document information on the use and management of medicinal plants by the people of Damot Woyde District. Fifty-seven medicinal plant species belonging to 31 families were collected, which are used by the study area inhabitants to treat various diseases in humans and livestock. The leading family was Asteraceae which was represented by 7 species (12.3%), followed by Rutaceae (6 species, 10.5%) and Solanaceae (5 species, 9%). Of the 57 medicinal plants collected, 36 (63.2%) were used to treat human ailments only, while 6 (10.5%) plant species were used to treat livestock ailments only and 15 (26.31%) were used to treat both human and livestock ailments. Herbs constituted the largest number of 22 species (38.6%), followed by shrubs 18 species (31.6%), trees 15 species (26.3%) and climbers 2 species (3.51%). Leaves (31.3%) were the most commonly used plant parts of preparing traditional remedies in the study area. Oral administration was the predominant mode of administration accounting for 71%. Preference ranking analysis revealed that Allium sativum L. was the most preferred plant species for treating the common cold. When the direct matrix ranking was analyzed, Croton macrostachyus Hochst. ex. Del. was the most commonly used medicinal plant for various purposes. Our finding revealed that plant species' use plays a vital role in treating human and animal diseases in Damot Woyde District. Phytochemical and pharmacological tests are recommended mainly on frequently used medicinal plants.
{"title":"Ethnobotanical study of medicinal plants used by local communities of Damot Woyde District, Wolaita Zone, Southern Ethiopia","authors":"M. Megersa, Samuel Woldetsadik","doi":"10.13057/nusbiosci/n140102","DOIUrl":"https://doi.org/10.13057/nusbiosci/n140102","url":null,"abstract":"Abstract. Megersa M, Woldetsadik S. 2022. Ethnobotanical study of medicinal plants used by local communities of Damot Woyde District, Wolaita Zone, Southern Ethiopia. Nusantara Bioscience 14: 10-24. Humans have used traditional medicines mainly of plant origins to treat diseases. Early humans faced a tremendous challenge when searching for natural products used as medicines. This study reports on an ethnobotanical study that focused on the traditional medicinal plants used by local communities to treat human and livestock diseases. An ethnobotanical study on medicinal plants was conducted from February 2020 to October 2020. This involved semi-structured interviews, field observations, market surveys and group discussions with informants to document information on the use and management of medicinal plants by the people of Damot Woyde District. Fifty-seven medicinal plant species belonging to 31 families were collected, which are used by the study area inhabitants to treat various diseases in humans and livestock. The leading family was Asteraceae which was represented by 7 species (12.3%), followed by Rutaceae (6 species, 10.5%) and Solanaceae (5 species, 9%). Of the 57 medicinal plants collected, 36 (63.2%) were used to treat human ailments only, while 6 (10.5%) plant species were used to treat livestock ailments only and 15 (26.31%) were used to treat both human and livestock ailments. Herbs constituted the largest number of 22 species (38.6%), followed by shrubs 18 species (31.6%), trees 15 species (26.3%) and climbers 2 species (3.51%). Leaves (31.3%) were the most commonly used plant parts of preparing traditional remedies in the study area. Oral administration was the predominant mode of administration accounting for 71%. Preference ranking analysis revealed that Allium sativum L. was the most preferred plant species for treating the common cold. When the direct matrix ranking was analyzed, Croton macrostachyus Hochst. ex. Del. was the most commonly used medicinal plant for various purposes. Our finding revealed that plant species' use plays a vital role in treating human and animal diseases in Damot Woyde District. Phytochemical and pharmacological tests are recommended mainly on frequently used medicinal plants.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49534951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-15DOI: 10.13057/nusbiosci/n140103
Ihsan Alrubaie, A. Salim, Mohauman Mohammad Majeed, A. Radhi
Abstract. Alrubaie I, Salim AT, Majeed MM, Radhi AJ. 2022. Synthesis of novel polymer quaternary ammonium salt derived from glucose as a phase transfer catalyst. Nusantara Bioscience 14: 25-33. Some new polymer containing quaternary ammonium salts based-carbohydrate starting from methyl-4,6-O-benzylidene-glucopyranoside have been prepared. The ammonium groups are connected to the glucose structure in various positions (2 and 3). Our synthesis used methyl-4,6-O-benzylidene-a-D-glucopyranoside through nucleophilic reagents to produce the main intermediates. The monomer glucose- quaternary ammonium salts are immobilized on the polyvinyl azide to give the final structure of the Polymer-Supported quaternary ammonium salts. In certain new kinds of phase transfer catalysts, the effectiveness of the polymer glucose-quaternary ammonium salts was tested in the Williamson etherification. The ether synthesis is a typical example of using a phase transfer catalyst. The reactions were performed in a liquid-liquid two-phase system, applying a mixture of toluene and 50% solution of aqueous sodium hydroxide in the presence of a 20 mg/mmol catalyst (compound 9). Compared to other derivatives, the reaction of 4-nitrophenol with dibromoethylene gives the highest yields. On the other hand, compound 9 with the C16 chain gives high activity in the phase transfer catalyst.
{"title":"Synthesis of novel polymer quaternary ammonium salt derived from glucose as a phase transfer catalyst","authors":"Ihsan Alrubaie, A. Salim, Mohauman Mohammad Majeed, A. Radhi","doi":"10.13057/nusbiosci/n140103","DOIUrl":"https://doi.org/10.13057/nusbiosci/n140103","url":null,"abstract":"Abstract. Alrubaie I, Salim AT, Majeed MM, Radhi AJ. 2022. Synthesis of novel polymer quaternary ammonium salt derived from glucose as a phase transfer catalyst. Nusantara Bioscience 14: 25-33. Some new polymer containing quaternary ammonium salts based-carbohydrate starting from methyl-4,6-O-benzylidene-glucopyranoside have been prepared. The ammonium groups are connected to the glucose structure in various positions (2 and 3). Our synthesis used methyl-4,6-O-benzylidene-a-D-glucopyranoside through nucleophilic reagents to produce the main intermediates. The monomer glucose- quaternary ammonium salts are immobilized on the polyvinyl azide to give the final structure of the Polymer-Supported quaternary ammonium salts. In certain new kinds of phase transfer catalysts, the effectiveness of the polymer glucose-quaternary ammonium salts was tested in the Williamson etherification. The ether synthesis is a typical example of using a phase transfer catalyst. The reactions were performed in a liquid-liquid two-phase system, applying a mixture of toluene and 50% solution of aqueous sodium hydroxide in the presence of a 20 mg/mmol catalyst (compound 9). Compared to other derivatives, the reaction of 4-nitrophenol with dibromoethylene gives the highest yields. On the other hand, compound 9 with the C16 chain gives high activity in the phase transfer catalyst.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45127311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-07DOI: 10.13057/nusbiosci/n140101
V. Cuevas, C. G. Banaay
Abstract. Cuevas VC, Banaay CGB. 2022. Trichoderma inoculants and straw compost improved resilience and yield in Cu-contaminated rice paddies. Nusantara Bioscience 14: 1-9. Rice paddies in Marinduque, Philippines, are copper-contaminated from tailings of two mining companies formerly operating in the province. At present, paddy-soil copper concentration ranges from 22-386 mg kg-1. Crops suffer from copper toxicity and water stress due to climate-related events. The field study was conducted to investigate the ability of in situ composted rice straw and Trichoderma microbial inoculant (TMI) to mitigate rice productivity constraints. In treated set-ups, rice straw was scattered on the paddy after harvest. Triple 14 mineral fertilizer was mixed with Trichoderma compost activator, broadcasted over the straws, and subsequently incorporated into the soil during land preparation. Rice seeds were TMI-coated before sowing. Rice straw composting was not done in control set-ups, and seeds were uncoated. Mineral fertilizers were applied to both set-ups. Furthermore, set-ups were categorized based on soil Cu content, such as normal, moderate, and high. Four replicates were made per season and category. In treated paddies, rice leaves did not show yellowing, indicating adequate N mineralization and plant uptake. The difference in yield was significantly higher (81%) in treated paddies compared to control. During severe drought conditions, the mean yield in treated paddies was 1.8 t ha-1, while that of control paddies was zero. The yield was significantly correlated with K inputs mainly by compost in treated paddies. The application of rice straw compost and Trichoderma inoculants can be an adaptive strategy for climate change resilience and mitigation of copper toxicity in crops.
{"title":"Trichoderma inoculants and straw compost improved resilience and yield in Cu-contaminated rice paddies","authors":"V. Cuevas, C. G. Banaay","doi":"10.13057/nusbiosci/n140101","DOIUrl":"https://doi.org/10.13057/nusbiosci/n140101","url":null,"abstract":"Abstract. Cuevas VC, Banaay CGB. 2022. Trichoderma inoculants and straw compost improved resilience and yield in Cu-contaminated rice paddies. Nusantara Bioscience 14: 1-9. Rice paddies in Marinduque, Philippines, are copper-contaminated from tailings of two mining companies formerly operating in the province. At present, paddy-soil copper concentration ranges from 22-386 mg kg-1. Crops suffer from copper toxicity and water stress due to climate-related events. The field study was conducted to investigate the ability of in situ composted rice straw and Trichoderma microbial inoculant (TMI) to mitigate rice productivity constraints. In treated set-ups, rice straw was scattered on the paddy after harvest. Triple 14 mineral fertilizer was mixed with Trichoderma compost activator, broadcasted over the straws, and subsequently incorporated into the soil during land preparation. Rice seeds were TMI-coated before sowing. Rice straw composting was not done in control set-ups, and seeds were uncoated. Mineral fertilizers were applied to both set-ups. Furthermore, set-ups were categorized based on soil Cu content, such as normal, moderate, and high. Four replicates were made per season and category. In treated paddies, rice leaves did not show yellowing, indicating adequate N mineralization and plant uptake. The difference in yield was significantly higher (81%) in treated paddies compared to control. During severe drought conditions, the mean yield in treated paddies was 1.8 t ha-1, while that of control paddies was zero. The yield was significantly correlated with K inputs mainly by compost in treated paddies. The application of rice straw compost and Trichoderma inoculants can be an adaptive strategy for climate change resilience and mitigation of copper toxicity in crops.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46537944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-11DOI: 10.13057/nusbiosci/n130216
Julinda Romauli Manullang, R. Nugroho, M. Rohmah, R. Rudianto, Amanda Qorysuchi
Abstract. Manullang JR, Nugroho RA, Rohmah M, Rudianto, Qorysuchi A. 2021. Plant-extract-mediated biosynthesis of silver nanoparticles using Eleutherine americana bulb extract and its characterization. Nusantara Bioscience 13: 247-254. The plant-based biosynthesis of nanoparticles has gained increasing momentum due to being lower in cost and eco-friendly. This study aimed to biosynthesize nanoparticles from the bulb ethanolic extract of Eleutherine americana (Aubl.) Merr. ex K.Heyne (Ea-AgNPs), then characterize Ea-AgNPs and determine their phytochemical content and antioxidant capacity. The Ea-AgNPs were synthesized using ethanolic extract of E. americana bulb along with various concentrations of AgNO3 (0.5-4 mM). The Ea-AgNPs were then characterized using UV-VIS spectroscopic, Scanning Electron Microscopy/ Energy Dispersive X-ray (SEM/EDX), Transmission Electron Microscopy (TEM), X-ray Powder Diffractometry (XRD), and Fourier-Transform Infrared Spectroscopy (FTIR) techniques. The results indicated that E. americana can be used to reduce AgNO3 to synthesize Ea-AgNPs, indicated by color change, and had optimum UV/VIS spectra at 400 nm. The FTIR analysis found that Ea-AgNPs showed peaks at 2919, 2850, 1586, and 1031 cm1, containing several important biocompounds. Additionally, the XRD results found an amorphous Ea-AgNP peak with maximum intensity and proportion of silver occurring at 24 Theta. The particle size distribution curve of Ea-AgNPs showed a size of 105 nm. Furthermore, SEM/EDX analysis revealed an optical absorption characteristic peak at 3 keV. The EDX examination revealed three signals: a strong signal from the C atom (70.99 %), an O atom (28.95 %), and Ag atom (0.06 %). The TEM imaging also showed the characteristics of Ea-AgNPs. Some phytochemicals such as flavonoids, tannins, alkaloids, and saponins were found in Ea-AgNPs, with IC50 values of 45.30 ppm.
{"title":"Plant-extract-mediated biosynthesis of silver nanoparticles using Eleutherine americana bulb extract and its characterization","authors":"Julinda Romauli Manullang, R. Nugroho, M. Rohmah, R. Rudianto, Amanda Qorysuchi","doi":"10.13057/nusbiosci/n130216","DOIUrl":"https://doi.org/10.13057/nusbiosci/n130216","url":null,"abstract":"Abstract. Manullang JR, Nugroho RA, Rohmah M, Rudianto, Qorysuchi A. 2021. Plant-extract-mediated biosynthesis of silver nanoparticles using Eleutherine americana bulb extract and its characterization. Nusantara Bioscience 13: 247-254. The plant-based biosynthesis of nanoparticles has gained increasing momentum due to being lower in cost and eco-friendly. This study aimed to biosynthesize nanoparticles from the bulb ethanolic extract of Eleutherine americana (Aubl.) Merr. ex K.Heyne (Ea-AgNPs), then characterize Ea-AgNPs and determine their phytochemical content and antioxidant capacity. The Ea-AgNPs were synthesized using ethanolic extract of E. americana bulb along with various concentrations of AgNO3 (0.5-4 mM). The Ea-AgNPs were then characterized using UV-VIS spectroscopic, Scanning Electron Microscopy/ Energy Dispersive X-ray (SEM/EDX), Transmission Electron Microscopy (TEM), X-ray Powder Diffractometry (XRD), and Fourier-Transform Infrared Spectroscopy (FTIR) techniques. The results indicated that E. americana can be used to reduce AgNO3 to synthesize Ea-AgNPs, indicated by color change, and had optimum UV/VIS spectra at 400 nm. The FTIR analysis found that Ea-AgNPs showed peaks at 2919, 2850, 1586, and 1031 cm1, containing several important biocompounds. Additionally, the XRD results found an amorphous Ea-AgNP peak with maximum intensity and proportion of silver occurring at 24 Theta. The particle size distribution curve of Ea-AgNPs showed a size of 105 nm. Furthermore, SEM/EDX analysis revealed an optical absorption characteristic peak at 3 keV. The EDX examination revealed three signals: a strong signal from the C atom (70.99 %), an O atom (28.95 %), and Ag atom (0.06 %). The TEM imaging also showed the characteristics of Ea-AgNPs. Some phytochemicals such as flavonoids, tannins, alkaloids, and saponins were found in Ea-AgNPs, with IC50 values of 45.30 ppm.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43002468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-03DOI: 10.13057/nusbiosci/n130214
Firman Heru Kurniawan, Lutfian Nazar, Rini Anjarwati, Herbowo Dwi Sasono, M. Rahayuningsih
Abstract. Kurniawan FH, Nazar L, Anjarwati R, Sasono HD, Rahayuningsih M. 2021. Orchids of Mount Ungaran (Indonesia) compiled from a decade of data collections between 2010 and 2021. Nusantara Bioscience 13: 245-259. Orchid is well known as a plant group widely used as ornamental plants because of their uniqueness. But, high exploitation, deforestation, and land-use changes have threatened orchid species and triggered extinction. It also happened in Mount Ungaran, Central Java, Indonesia, despite designation as an essential ecosystem area. This research was conducted to add an updated list of wild orchid species that can serve as biodiversity baseline data for conservation management in Mount Ungaran. The study was carried out through exploration activities from 2010 to 2021. The study area covered eleven sites namely Gentong Hills, Kalisidi, Pasigitan, Banyuwindu, Gajahmungkur, Watuondo, Mawar Camp, Indrakila, Gondang and Puncak which were all included in the buffer zone of Mount Ungaran. A total of 115 species from 54 genera of orchids were successfully identified, including 78 species of epiphyte and 37 species of terrestrial orchids. Among the species recorded, at least 27 species were known to be endemic to Indonesia, and 8 species were endemic to the island of Java, which four species were listed in the IUCN Red List. In addition, all the species found were listed in Appendix II of the CITES, except one species, Paphiopedilum javanicum which was listed in Appendix I. This study recorded the new distribution records of several species of orchids that were previously only found in West Java or East Java, i.e., Ania penangiana, Pholidota convallariae, and Crepidium junghuhnii. This study has also documented a species from genus Crepidium, which morphological characters have not been described in any identification guides. The Gentong area has potential as a priority for in-situ orchid protection area by the highest number of species were reached 54% of all orchids found on Mount Ungaran.
{"title":"Orchids of Mount Ungaran (Indonesia) compiled from a decade of data collections between 2010 and 2021","authors":"Firman Heru Kurniawan, Lutfian Nazar, Rini Anjarwati, Herbowo Dwi Sasono, M. Rahayuningsih","doi":"10.13057/nusbiosci/n130214","DOIUrl":"https://doi.org/10.13057/nusbiosci/n130214","url":null,"abstract":"Abstract. Kurniawan FH, Nazar L, Anjarwati R, Sasono HD, Rahayuningsih M. 2021. Orchids of Mount Ungaran (Indonesia) compiled from a decade of data collections between 2010 and 2021. Nusantara Bioscience 13: 245-259. Orchid is well known as a plant group widely used as ornamental plants because of their uniqueness. But, high exploitation, deforestation, and land-use changes have threatened orchid species and triggered extinction. It also happened in Mount Ungaran, Central Java, Indonesia, despite designation as an essential ecosystem area. This research was conducted to add an updated list of wild orchid species that can serve as biodiversity baseline data for conservation management in Mount Ungaran. The study was carried out through exploration activities from 2010 to 2021. The study area covered eleven sites namely Gentong Hills, Kalisidi, Pasigitan, Banyuwindu, Gajahmungkur, Watuondo, Mawar Camp, Indrakila, Gondang and Puncak which were all included in the buffer zone of Mount Ungaran. A total of 115 species from 54 genera of orchids were successfully identified, including 78 species of epiphyte and 37 species of terrestrial orchids. Among the species recorded, at least 27 species were known to be endemic to Indonesia, and 8 species were endemic to the island of Java, which four species were listed in the IUCN Red List. In addition, all the species found were listed in Appendix II of the CITES, except one species, Paphiopedilum javanicum which was listed in Appendix I. This study recorded the new distribution records of several species of orchids that were previously only found in West Java or East Java, i.e., Ania penangiana, Pholidota convallariae, and Crepidium junghuhnii. This study has also documented a species from genus Crepidium, which morphological characters have not been described in any identification guides. The Gentong area has potential as a priority for in-situ orchid protection area by the highest number of species were reached 54% of all orchids found on Mount Ungaran.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45541792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-02DOI: 10.13057/nusbiosci/n130213
S. H. Saputra, B. Saragih, I. Kusuma, E. Arung
Abstract. Saputra SH, Saragih B, Kusuma IW, Arung ET. 2021. Antioxidant and antibacterial screening of honey of Hiterotrogona itama collected from differents meliponiculture areas in East Kalimantan, Indonesia. Nusantara Bioscience 13: 232-237. Heterotrigona itama (Cockerell, 1918) is a stingless bee that exits in several areas in East Kalimantan of Indonesia. This study aimed to analyze the phytochemicals, antioxidant activity of honey of this stingless bee from several regions in East Kalimantan. Phytochemical testing was carried out qualitatively, while antioxidant activity was performed using DPPH (1.1-diphenyl-2-picrylhydrazyl-radical) radical scavenging assay. The antimicrobial activity of Staphylococcus aureus and Escherichia coli was performed through in vitro test. The results showed that the honey of stingless H. itama bee collected from five cultivated areas in East Kalimantan contained flavonoid, coumarin, steroids, carotenoids, and some of which had alkaloid and tannin compounds. The antioxidant activity (IC50) was observed between 43,54 ppm-71,27 ppm, while the strong antimicrobial activity of this honey was found against S. aureus and E. coli.
{"title":"Antioxidant and antibacterial screening of honey of Hiterotrogona itama collected from differents meliponiculture areas in East Kalimantan, Indonesia","authors":"S. H. Saputra, B. Saragih, I. Kusuma, E. Arung","doi":"10.13057/nusbiosci/n130213","DOIUrl":"https://doi.org/10.13057/nusbiosci/n130213","url":null,"abstract":"Abstract. Saputra SH, Saragih B, Kusuma IW, Arung ET. 2021. Antioxidant and antibacterial screening of honey of Hiterotrogona itama collected from differents meliponiculture areas in East Kalimantan, Indonesia. Nusantara Bioscience 13: 232-237. Heterotrigona itama (Cockerell, 1918) is a stingless bee that exits in several areas in East Kalimantan of Indonesia. This study aimed to analyze the phytochemicals, antioxidant activity of honey of this stingless bee from several regions in East Kalimantan. Phytochemical testing was carried out qualitatively, while antioxidant activity was performed using DPPH (1.1-diphenyl-2-picrylhydrazyl-radical) radical scavenging assay. The antimicrobial activity of Staphylococcus aureus and Escherichia coli was performed through in vitro test. The results showed that the honey of stingless H. itama bee collected from five cultivated areas in East Kalimantan contained flavonoid, coumarin, steroids, carotenoids, and some of which had alkaloid and tannin compounds. The antioxidant activity (IC50) was observed between 43,54 ppm-71,27 ppm, while the strong antimicrobial activity of this honey was found against S. aureus and E. coli.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45525470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-09DOI: 10.13057/nusbiosci/n130210
C. Bailly
Abstract. Bailly C. 2021. Review: Natural products isolated from Portulaca oleracea (purslane, Ma-Chi-Xian): Focus on oleraciamides and oleracones. Nusantara Bioscience 13: 202-210. The plant Portulaca oleracea L., known as purslane in English and Ma-Chi-Xian in Chinese, is largely used in traditional medicine to treat a variety of diseases and conditions, including dysentery, urinary tract dysfunctions, and post-partum bleeding. It is also an edible plant, with a high nutritional potential. Extracts of P. oleracea display antioxidant, anti-inflammatory and antiproliferative activities, associated with the presence of numerous bioactive secondary metabolites, including alkaloids, terpenoids, lignans and polysaccharides. The present review provides a specific focus on two sub-groups of natural products isolated in recent years from P. oleracea: the alkaloids oleraciamides A-to-G, and the oleracones A-to-L which are mostly flavonoids. Their structural diversity and pharmacological properties, described in recent publications and patents, have been analyzed. These two subgroups of natural products deserve additional studies to delineate their mechanism of action. They could serve as a starting point to the design of novel anti-inflammatory agents, at least for some of them. This review provides a global view of these compounds, necessary to promote further phytochemical studies and to better apprehend the traditional use of the plant and its extracts.
{"title":"Review: Natural products isolated from Portulaca oleracea (purslane, Ma-Chi-Xian): Focus on oleraciamides and oleracones","authors":"C. Bailly","doi":"10.13057/nusbiosci/n130210","DOIUrl":"https://doi.org/10.13057/nusbiosci/n130210","url":null,"abstract":"Abstract. Bailly C. 2021. Review: Natural products isolated from Portulaca oleracea (purslane, Ma-Chi-Xian): Focus on oleraciamides and oleracones. Nusantara Bioscience 13: 202-210. The plant Portulaca oleracea L., known as purslane in English and Ma-Chi-Xian in Chinese, is largely used in traditional medicine to treat a variety of diseases and conditions, including dysentery, urinary tract dysfunctions, and post-partum bleeding. It is also an edible plant, with a high nutritional potential. Extracts of P. oleracea display antioxidant, anti-inflammatory and antiproliferative activities, associated with the presence of numerous bioactive secondary metabolites, including alkaloids, terpenoids, lignans and polysaccharides. The present review provides a specific focus on two sub-groups of natural products isolated in recent years from P. oleracea: the alkaloids oleraciamides A-to-G, and the oleracones A-to-L which are mostly flavonoids. Their structural diversity and pharmacological properties, described in recent publications and patents, have been analyzed. These two subgroups of natural products deserve additional studies to delineate their mechanism of action. They could serve as a starting point to the design of novel anti-inflammatory agents, at least for some of them. This review provides a global view of these compounds, necessary to promote further phytochemical studies and to better apprehend the traditional use of the plant and its extracts.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43739051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-03DOI: 10.13057/nusbiosci/n130207
A. Ghosh, Soumya Majumder, S. Saha, S. Sarkar, M. Bhattacharya
Abstract. Ghosh A, Majumder S, Saha S, Sarkar S, Bhattacharya M. 2021. Gas Chromatography-Mass Spectrometry profiling, and evaluation of antioxidant and antibacterial activity of Albizia spp. Nusantara Bioscience 13: 177-184. Plant resources have been utilized for human welfare since ancient times. Most of the pharmaceutical industry relies on bioactive molecules from plant resources. This research was carried out to investigate the antioxidant and antibacterial activities of Albizia spp. DPPH assay was conducted to investigate the antioxidant activity and well diffusion test by pour plate method was followed for antibacterial assay. Antioxidant activity confirmed that the leaf extracts with chemical contents having solubility in polar solvents showed high free radical scavenging activity. The free radical scavenging activities of bark extracts were lower than the leaf extracts. Leaf extracts of Albizia procera showed the highest scavenging activity (94.77% or 100.66 µg AE/ ml); followed by Albizia odoratissima (93.52% or 99.23µg AE/ ml); Albizia lebbeck (91.68% or 97.13 µg AE/ ml) and Albizia chinensis (89.91% or 95.11 µg AE/ ml). GC-MS analysis of leaf and bark extracts revealed that 15 compounds had been reported to have antioxidant activities such as beta-amyrin, phytol, squalene, vitamin E and 31 compounds had been reported to have antimicrobial, antibacterial, antifungal, and antiviral activity. The antibacterial activities of leaf and bark extracts of four Albizia species showed that leaf extracts of Albizia spp. showed better antibacterial activity compared to bark samples which were compatible with the results found from GC-MS analysis.
{"title":"Gas Chromatography-Mass Spectrometry profiling, and evaluation of antioxidant and antibacterial activity of Albizia spp.","authors":"A. Ghosh, Soumya Majumder, S. Saha, S. Sarkar, M. Bhattacharya","doi":"10.13057/nusbiosci/n130207","DOIUrl":"https://doi.org/10.13057/nusbiosci/n130207","url":null,"abstract":"Abstract. Ghosh A, Majumder S, Saha S, Sarkar S, Bhattacharya M. 2021. Gas Chromatography-Mass Spectrometry profiling, and evaluation of antioxidant and antibacterial activity of Albizia spp. Nusantara Bioscience 13: 177-184. Plant resources have been utilized for human welfare since ancient times. Most of the pharmaceutical industry relies on bioactive molecules from plant resources. This research was carried out to investigate the antioxidant and antibacterial activities of Albizia spp. DPPH assay was conducted to investigate the antioxidant activity and well diffusion test by pour plate method was followed for antibacterial assay. Antioxidant activity confirmed that the leaf extracts with chemical contents having solubility in polar solvents showed high free radical scavenging activity. The free radical scavenging activities of bark extracts were lower than the leaf extracts. Leaf extracts of Albizia procera showed the highest scavenging activity (94.77% or 100.66 µg AE/ ml); followed by Albizia odoratissima (93.52% or 99.23µg AE/ ml); Albizia lebbeck (91.68% or 97.13 µg AE/ ml) and Albizia chinensis (89.91% or 95.11 µg AE/ ml). GC-MS analysis of leaf and bark extracts revealed that 15 compounds had been reported to have antioxidant activities such as beta-amyrin, phytol, squalene, vitamin E and 31 compounds had been reported to have antimicrobial, antibacterial, antifungal, and antiviral activity. The antibacterial activities of leaf and bark extracts of four Albizia species showed that leaf extracts of Albizia spp. showed better antibacterial activity compared to bark samples which were compatible with the results found from GC-MS analysis.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47677811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-17DOI: 10.13057/nusbiosci/n130206
S. Purnami, Indri Permata Wibisari, Viria Agesti Suvifan, S. Nurhayati, D. Ramadhani
Abstract. Purnami S, Wibisari IP, Suvifan VA, Nurhayati S, Ramadhani D. 2021. Effects of carbon dioxide purities on mitotic index in lymphocyte culture and metaphase chromosome quality. Nusantara Bioscience 13: 171-176. The metaphase chromosome spread quality is necessary for a faster individual dose prediction following radiological accidents using dicentric chromosome assay. It is well known that the low-quality metaphase chromosome spreads can lead to false positives of dicentric chromosome identification. Thus, evaluation of the main variable that influences the preparation of high-quality metaphase chromosome spreads is important to perform. Until now, no studies have assessed the effects of CO2 purities on metaphase chromosome spread quality. This study aimed to evaluate the effects of carbon dioxide (CO2) purities on lymphocyte proliferation, and the quality of metaphase chromosome spreads to improve the chromosome aberration assay for cytogenetic biodosimetry purposes. Whole blood samples from three different subjects were cultured and incubated for 48 hours with two different grades of CO2 (high purity and food grades) and without CO2. The mitotic index (MI) from each subject was assessed, and the quality of metaphase chromosome spreads was evaluated by comparing the lengths of chromosomes 1, 2, and 21. Statistical analysis revealed that the difference between manual and automatic MI under three different conditions of CO2 purity was not statistically significant (p = 0.162; p = 0.901). Comparative analysis of the lengths of chromosomes 1, 2, and 21 from 145 metaphases also showed a difference that was not statistically significant (p = 0.745; p = 0.915; p = 0.399). Overall, our findings suggest that CO2 purities do not impair lymphocyte proliferation or metaphase quality. Further investigation should include other technical improvements such as drop-slide optimization.
{"title":"Effects of carbon dioxide purities on mitotic index in lymphocyte culture and metaphase chromosome quality","authors":"S. Purnami, Indri Permata Wibisari, Viria Agesti Suvifan, S. Nurhayati, D. Ramadhani","doi":"10.13057/nusbiosci/n130206","DOIUrl":"https://doi.org/10.13057/nusbiosci/n130206","url":null,"abstract":"Abstract. Purnami S, Wibisari IP, Suvifan VA, Nurhayati S, Ramadhani D. 2021. Effects of carbon dioxide purities on mitotic index in lymphocyte culture and metaphase chromosome quality. Nusantara Bioscience 13: 171-176. The metaphase chromosome spread quality is necessary for a faster individual dose prediction following radiological accidents using dicentric chromosome assay. It is well known that the low-quality metaphase chromosome spreads can lead to false positives of dicentric chromosome identification. Thus, evaluation of the main variable that influences the preparation of high-quality metaphase chromosome spreads is important to perform. Until now, no studies have assessed the effects of CO2 purities on metaphase chromosome spread quality. This study aimed to evaluate the effects of carbon dioxide (CO2) purities on lymphocyte proliferation, and the quality of metaphase chromosome spreads to improve the chromosome aberration assay for cytogenetic biodosimetry purposes. Whole blood samples from three different subjects were cultured and incubated for 48 hours with two different grades of CO2 (high purity and food grades) and without CO2. The mitotic index (MI) from each subject was assessed, and the quality of metaphase chromosome spreads was evaluated by comparing the lengths of chromosomes 1, 2, and 21. Statistical analysis revealed that the difference between manual and automatic MI under three different conditions of CO2 purity was not statistically significant (p = 0.162; p = 0.901). Comparative analysis of the lengths of chromosomes 1, 2, and 21 from 145 metaphases also showed a difference that was not statistically significant (p = 0.745; p = 0.915; p = 0.399). Overall, our findings suggest that CO2 purities do not impair lymphocyte proliferation or metaphase quality. Further investigation should include other technical improvements such as drop-slide optimization.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45832272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-28DOI: 10.13057/nusbiosci/n130203
A. Haq, S. Saeed, Alia Ahmed
Abstract. Haq AU, Saeed S, Ahmed A. 2021. Species diversity and ethnobotanical inventory of wild flora used by the folk community of Shinghar Balochistan, Pakistan. Nusantara Bioscience 13: 148-157. Upper Suleiman Mountain of Shinghar Balochistan, Pakistan is little known, the structural and floristic composition are poorly understood. The dominant socio-linguistic groups of the area are Pashtoon tribes, having a long history of medicinal plants utilization as a part of their indigenous primary health care system. The present study was carried out to describe the plant species diversity of Shinghar, and to the inventory of some important wild medicinal, edible and endemic plants of the region. A total of 102 species belonging to 42 families and 88 genera were recorded. Herbs were dominant comprising 62% of documented plants, followed by shrubs (26%), and trees (14%). Medicinal plants of the area are being used in treatments of many ailments like joint pains, stomach problems, skin allergies and inflammation.. Edible plants were 14% of total recorded plants. The documented plants were also used for other use-categories than medicinal and edible purposes including fodder, fencing, and ornamental. In addition, some of the medicinal plants were considered poisonous but used for medicine. Elevation of the area ranged from 1700 to 3400 meters above sea level. The dominating tree was Pinus gerardiana, and the area is also known as the Chilghoza Forest. Asteraceae was the dominating family in the area with 14 species, followed by Lamiaceae (7 species), Poaceae (6 species), Solanaceae, Leguminosae, Fabaceae, and Apocynaceae with 5 species each. The results revealed the importance of endemic and endangered plants of area which are need to be conserve. Moreover, the present study highlighted species diversity not earlier described from high altitudes.
{"title":"Species diversity and ethnobotanical inventory of wild flora used by the folk community of Shinghar Balochistan, Pakistan","authors":"A. Haq, S. Saeed, Alia Ahmed","doi":"10.13057/nusbiosci/n130203","DOIUrl":"https://doi.org/10.13057/nusbiosci/n130203","url":null,"abstract":"Abstract. Haq AU, Saeed S, Ahmed A. 2021. Species diversity and ethnobotanical inventory of wild flora used by the folk community of Shinghar Balochistan, Pakistan. Nusantara Bioscience 13: 148-157. Upper Suleiman Mountain of Shinghar Balochistan, Pakistan is little known, the structural and floristic composition are poorly understood. The dominant socio-linguistic groups of the area are Pashtoon tribes, having a long history of medicinal plants utilization as a part of their indigenous primary health care system. The present study was carried out to describe the plant species diversity of Shinghar, and to the inventory of some important wild medicinal, edible and endemic plants of the region. A total of 102 species belonging to 42 families and 88 genera were recorded. Herbs were dominant comprising 62% of documented plants, followed by shrubs (26%), and trees (14%). Medicinal plants of the area are being used in treatments of many ailments like joint pains, stomach problems, skin allergies and inflammation.. Edible plants were 14% of total recorded plants. The documented plants were also used for other use-categories than medicinal and edible purposes including fodder, fencing, and ornamental. In addition, some of the medicinal plants were considered poisonous but used for medicine. Elevation of the area ranged from 1700 to 3400 meters above sea level. The dominating tree was Pinus gerardiana, and the area is also known as the Chilghoza Forest. Asteraceae was the dominating family in the area with 14 species, followed by Lamiaceae (7 species), Poaceae (6 species), Solanaceae, Leguminosae, Fabaceae, and Apocynaceae with 5 species each. The results revealed the importance of endemic and endangered plants of area which are need to be conserve. Moreover, the present study highlighted species diversity not earlier described from high altitudes.","PeriodicalId":19481,"journal":{"name":"Nusantara Bioscience","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2021-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47538047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：