首页 > 最新文献

Open Veterinary Journal最新文献

英文 中文
Abdominal fat necrosis in a female dromedary camel: Clinical, hematobiochemical, sonographic, and pathologic findings. 一只雌性单峰骆驼的腹部脂肪坏死:临床、血液生化、声像图和病理学发现。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-07-01 Epub Date: 2024-07-31 DOI: 10.5455/OVJ.2024.v14.i7.22
Mohamed Tharwat, Mohie Haridy, Hazem M M Elmoghazy, Ayman Elnahas, Abdulrahman A Alkheraif

Background: Abdominal fat necrosis is mostly an incidental finding during rectal examination manifested by the presence of hard necrotic masses of different sizes. Differential diagnosis includes gastrointestinal neoplasia, abdominal abscessation and mummified fetuses. This report describes the clinical, hematological, biochemical, sonographic, and pathologic findings in a female Arabian camel (Camelus dromedaries) with abdominal fat necrosis.

Case description: A 7-year-old female dromedary camel was presented for examination because of weakness and progressive weight loss since months ago. Anthelmintics, analgesics, broad-spectrum antimicrobials, anti-inflammatories and stomachics were administered, but did not result in significant improvement. Preliminary examination of the case revealed emaciation with a body condition score of 2.5 (scale 1-5). The cardiopulmonary, digestive, and urinary systems were evaluated transcutaneously by sonography in the recumbent position. Multiple echogenic masses-located within the intestinal loops were observed alongside few hypoechogenic masses. A hyperechogenic peritoneal effusion and enlarged mesenteric lymph nodes were also detected. Decreased hematocrit value, normocytic normochromic anemia, neutrophilic leukocytosis, hypoalbuminemia, hhyperglobulinemia, hyperglycemia, hyponatremia, and hyperkalemia were detected. A free-hand, ultrasound-guided biopsy was examined histopathologically and revealed multiple abdominal fat necrosis, severe fibrosis, and pancreatitis.

Conclusion: It is concluded that, transabdominal ultrasonography in camels was valuable for suspicious of abdominal mesenteric masses and helpful in sampling of a representative biopsy specimen However, histological examination of the biopsy remains the final diagnostic method.

背景:腹部脂肪坏死大多是直肠检查中的偶然发现,表现为出现大小不一的硬性坏死肿块。鉴别诊断包括胃肠道肿瘤、腹腔脓肿和木乃伊化胎儿。本报告描述了一只患有腹部脂肪坏死的雌性阿拉伯骆驼(Camelus dromedaries)的临床、血液学、生物化学、声像图和病理学检查结果:一只 7 岁的雌性单峰骆驼因数月前出现虚弱和体重逐渐减轻而前来接受检查。医生给它服用了驱虫药、镇痛药、广谱抗菌药、消炎药和健胃药,但病情没有明显好转。对该病例的初步检查显示其身体消瘦,身体状况评分为 2.5(1-5 分)。患者在卧位时通过超声波检查对心肺、消化和泌尿系统进行了评估。除了几个低回声肿块外,还观察到位于肠襻内的多个回声肿块。还发现了高回声腹腔积液和肠系膜淋巴结肿大。此外,还发现血细胞比容值下降、正常红细胞性贫血、中性粒细胞白细胞增多、低白蛋白血症、高球蛋白血症、高血糖、低钠血症和高钾血症。在超声引导下进行的活组织病理学检查显示,患者腹部多处脂肪坏死、严重纤维化和胰腺炎:结论:骆驼经腹超声波检查对怀疑腹部肠系膜肿块很有价值,并有助于采集有代表性的活检标本。
{"title":"Abdominal fat necrosis in a female dromedary camel: Clinical, hematobiochemical, sonographic, and pathologic findings.","authors":"Mohamed Tharwat, Mohie Haridy, Hazem M M Elmoghazy, Ayman Elnahas, Abdulrahman A Alkheraif","doi":"10.5455/OVJ.2024.v14.i7.22","DOIUrl":"10.5455/OVJ.2024.v14.i7.22","url":null,"abstract":"<p><strong>Background: </strong>Abdominal fat necrosis is mostly an incidental finding during rectal examination manifested by the presence of hard necrotic masses of different sizes. Differential diagnosis includes gastrointestinal neoplasia, abdominal abscessation and mummified fetuses. This report describes the clinical, hematological, biochemical, sonographic, and pathologic findings in a female Arabian camel (<i>Camelus dromedaries</i>) with abdominal fat necrosis.</p><p><strong>Case description: </strong>A 7-year-old female dromedary camel was presented for examination because of weakness and progressive weight loss since months ago. Anthelmintics, analgesics, broad-spectrum antimicrobials, anti-inflammatories and stomachics were administered, but did not result in significant improvement. Preliminary examination of the case revealed emaciation with a body condition score of 2.5 (scale 1-5). The cardiopulmonary, digestive, and urinary systems were evaluated transcutaneously by sonography in the recumbent position. Multiple echogenic masses-located within the intestinal loops were observed alongside few hypoechogenic masses. A hyperechogenic peritoneal effusion and enlarged mesenteric lymph nodes were also detected. Decreased hematocrit value, normocytic normochromic anemia, neutrophilic leukocytosis, hypoalbuminemia, hhyperglobulinemia, hyperglycemia, hyponatremia, and hyperkalemia were detected. A free-hand, ultrasound-guided biopsy was examined histopathologically and revealed multiple abdominal fat necrosis, severe fibrosis, and pancreatitis.</p><p><strong>Conclusion: </strong>It is concluded that, transabdominal ultrasonography in camels was valuable for suspicious of abdominal mesenteric masses and helpful in sampling of a representative biopsy specimen However, histological examination of the biopsy remains the final diagnostic method.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11338617/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142036541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Efficiency of using lactic acid and beefxide to reduce indicator microorganisms on beef in Mosul slaughterhouse. 使用乳酸和牛肉氧化物减少摩苏尔屠宰场牛肉上指示性微生物的效率。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-07-01 Epub Date: 2024-07-31 DOI: 10.5455/OVJ.2024.v14.i7.15
Israa M Jweer, Omar A Al-Mahmood

Background: Meat contamination occurs in various ways, the most important of which are live animals before slaughter and the slaughter process (de-hiding and evisceration). For this, many substances were used that have an antimicrobial effect and can disinfect the surfaces of the carcass and extend its shelf life.

Aim: This research aimed to study the efficiency of using some organic acids (lactic acid and beefxide) to reduce the microbial load (indicator microorganisms) on the surfaces of beef carcasses and some edible organs in the Mosul slaughterhouse.

Methods: Two hundred sixty-four swabs (192 carcasses + 72 edible organ samples) were collected over the course of three months from the Mosul slaughterhouse in Nineveh Governorate between September 2023, and December 2023 (132 treated with organic acids and 132 not treated). The petrifilm method was used to detect indicator microorganisms in the samples.

Results: Our results showed that the contamination rate in beef carcasses with generic Escherichia coli, coliforms, total coliform counts, and Enterobacteriaceae before treatment was 0.81, 1.22, 1.48, and 1.38 mean log colony forming unit (CFU/cm2), respectively. While the contamination rate in samples treated with organic acids for generic E. coli, coliforms, total coliform counts, and Enterobacteriaceae was -0.1, 0.31, 0.45, and 0.41 mean log CFU/cm2, respectively. Moreover, the level of contamination with indicator microorganisms in edible organs treated with organic acids was lower compared to untreated samples. Even though there was contamination with indicator microorganisms in the liver, heart, and kidney, there was no "significant" difference between them. Whereas there was no significant difference (p > 0.05) between lactic acid and beefxide solution in terms of reducing the rate of contamination of the indicator microorganisms in carcasses and the edible organs samples. Regarding the type of swabs used in the study, the results showed the effectiveness of sponge swabs, as the rate of microbial recovery (indicator microorganisms) was higher (p < 0.01) compared to cotton swabs.

Conclusion: The study demonstrated the efficiency of using organic acids (lactic acid and beefxide solution) in reducing the microbial load to a level that does not cause diseases.

背景:肉类污染有多种途径,其中最重要的是屠宰前的活体动物和屠宰过程(去皮和去内脏)。目的:本研究旨在研究摩苏尔屠宰场使用一些有机酸(乳酸和牛肉酸)减少牛肉胴体和一些可食用器官表面微生物负荷(指示性微生物)的效率:在 2023 年 9 月至 2023 年 12 月的三个月期间,从尼尼微省摩苏尔屠宰场收集了 264 份拭子样本(192 份胴体样本 + 72 份食用器官样本)(132 份经有机酸处理,132 份未经处理)。采用 petrifilm 法检测样品中的指示微生物:结果表明,处理前牛肉胴体中普通大肠杆菌、大肠菌群、总大肠菌群和肠杆菌科细菌的污染率分别为 0.81、1.22、1.48 和 1.38 平均对数菌落形成单位(CFU/cm2)。而用有机酸处理过的样品中,普通大肠杆菌、大肠菌群、总大肠菌群和肠杆菌科细菌的污染率分别为-0.1、0.31、0.45 和 0.41 平均对数 CFU/cm2。此外,与未经处理的样本相比,经有机酸处理的食用器官中指示性微生物的污染水平较低。即使肝脏、心脏和肾脏受到指示微生物的污染,它们之间也没有 "显著 "差异。而乳酸和牛黄溶液在降低胴体和食用器官样品中指示微生物的污染率方面没有明显差异(p > 0.05)。关于研究中使用的拭子类型,结果表明海绵拭子的有效性,因为与棉拭子相比较,海绵拭子的微生物回收率(指示微生物)更高(p < 0.01):该研究表明,使用有机酸(乳酸和牛肉酸溶液)能有效地将微生物量减少到不会引起疾病的水平。
{"title":"Efficiency of using lactic acid and beefxide to reduce indicator microorganisms on beef in Mosul slaughterhouse.","authors":"Israa M Jweer, Omar A Al-Mahmood","doi":"10.5455/OVJ.2024.v14.i7.15","DOIUrl":"10.5455/OVJ.2024.v14.i7.15","url":null,"abstract":"<p><strong>Background: </strong>Meat contamination occurs in various ways, the most important of which are live animals before slaughter and the slaughter process (de-hiding and evisceration). For this, many substances were used that have an antimicrobial effect and can disinfect the surfaces of the carcass and extend its shelf life.</p><p><strong>Aim: </strong>This research aimed to study the efficiency of using some organic acids (lactic acid and beefxide) to reduce the microbial load (indicator microorganisms) on the surfaces of beef carcasses and some edible organs in the Mosul slaughterhouse.</p><p><strong>Methods: </strong>Two hundred sixty-four swabs (192 carcasses + 72 edible organ samples) were collected over the course of three months from the Mosul slaughterhouse in Nineveh Governorate between September 2023, and December 2023 (132 treated with organic acids and 132 not treated). The petrifilm method was used to detect indicator microorganisms in the samples.</p><p><strong>Results: </strong>Our results showed that the contamination rate in beef carcasses with generic <i>Escherichia coli</i>, coliforms, total coliform counts, and <i>Enterobacteriaceae</i> before treatment was 0.81, 1.22, 1.48, and 1.38 mean log colony forming unit (CFU/cm<sup>2</sup>), respectively. While the contamination rate in samples treated with organic acids for generic <i>E. coli</i>, coliforms, total coliform counts, and <i>Enterobacteriaceae</i> was -0.1, 0.31, 0.45, and 0.41 mean log CFU/cm<sup>2</sup>, respectively. Moreover, the level of contamination with indicator microorganisms in edible organs treated with organic acids was lower compared to untreated samples. Even though there was contamination with indicator microorganisms in the liver, heart, and kidney, there was no \"significant\" difference between them. Whereas there was no significant difference (<i>p</i> > 0.05) between lactic acid and beefxide solution in terms of reducing the rate of contamination of the indicator microorganisms in carcasses and the edible organs samples. Regarding the type of swabs used in the study, the results showed the effectiveness of sponge swabs, as the rate of microbial recovery (indicator microorganisms) was higher (<i>p</i> < 0.01) compared to cotton swabs.</p><p><strong>Conclusion: </strong>The study demonstrated the efficiency of using organic acids (lactic acid and beefxide solution) in reducing the microbial load to a level that does not cause diseases.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11338611/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142036547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phylogenetic analysis of Staphylococcus aureus enterotoxin A gene in Iraqi breed cows with bovine mastitis: Implications for disease management. 伊拉克种牛乳腺炎患者金黄色葡萄球菌肠毒素 A 基因的系统发育分析:对疾病管理的影响。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-07-01 Epub Date: 2024-07-31 DOI: 10.5455/OVJ.2024.v14.i7.14
Alaa Jawad, Sabreen Noori Dagman, Mohammed Mahdi Yaseen, Hassan Al-Karagoly

Background: Although milk is nutritionally valuable, it also serves as a significant medium for the transmission of pathogens and their toxins.

Aim: This study aimed to investigate the role of enterotoxin gene A (SEA) in the development of bovine mastitis. We accomplished this by examining milk through polymerase chain reaction (PCR) testing, amino acid substitution analysis, and phylogenetic analysis.

Methods: A total of fifty milk samples were collected from locally bred dairy cows in Al-Diwaniyah, located in southern Iraq. We employed the VITEK-2 platform to validate the diagnosis of Staphylococcus aureus and confirm the results of routine tests (culturing and biochemical tests). Subsequently, the genetic mutation and phylogeny analysis were achieved utilizing DNA sequencing to 16S rRNA and enterotoxin A genes.

Results: 66% (33/50) of the milk samples found to be contain S. aureus by the VITEK-2 system. Furthermore, 25/33 of the samples were positive by the PCR test. While 60% (15 out of 25) tested positive for the SEA gene. After genomic analysis, we identified amino acid substitutions of serine, glutamine with arginine, tyrosine with cysteine, and aspartic acid with glycine at positions 9, 101, 119, 187, and 191. The phylogenetic investigation demonstrated a genetic relationship between our isolates (Iraqi isolates) and isolates from Indian and the United States.

Conclusion: Our study indicated the widespread distribution of the enterotoxin gene A (SEA) of S. aureus among dairy cows. The molecular study revealed significant changes in key amino acids that could play an important role in the bacterium's pathogenesis. The phylogenetic similarities among S. aureus samples from various countries suggest that the bacteria has spread globally.

背景:目的:本研究旨在调查肠毒素基因 A (SEA) 在牛乳腺炎发病中的作用。为此,我们通过聚合酶链式反应(PCR)测试、氨基酸替代分析和系统发育分析对牛奶进行了检测:方法:我们从伊拉克南部迪瓦尼耶(Al-Diwaniyah)当地饲养的奶牛身上共采集了 50 份牛奶样本。我们使用 VITEK-2 平台验证了金黄色葡萄球菌的诊断,并确认了常规检测(培养和生化检测)的结果。随后,通过对 16S rRNA 和肠毒素 A 基因进行 DNA 测序,实现了基因突变和系统发育分析:结果:通过 VITEK-2 系统检测发现,66%(33/50)的牛奶样本中含有金黄色葡萄球菌。此外,25/33 的样本在 PCR 检测中呈阳性。60%(25 个样本中的 15 个)的 SEA 基因检测结果呈阳性。经过基因组分析,我们在第 9、101、119、187 和 191 位发现了丝氨酸、谷氨酰胺与精氨酸、酪氨酸与半胱氨酸、天冬氨酸与甘氨酸的氨基酸替换。系统发育调查表明,我们的分离株(伊拉克分离株)与来自印度和美国的分离株之间存在遗传关系:我们的研究表明,金黄色葡萄球菌的肠毒素基因 A(SEA)在奶牛中广泛分布。分子研究揭示了关键氨基酸的重大变化,这些变化可能在该细菌的致病过程中发挥重要作用。来自不同国家的金黄色葡萄球菌样本之间的系统发育相似性表明,该细菌已在全球范围内传播。
{"title":"Phylogenetic analysis of <i>Staphylococcus aureus</i> enterotoxin A gene in Iraqi breed cows with bovine mastitis: Implications for disease management.","authors":"Alaa Jawad, Sabreen Noori Dagman, Mohammed Mahdi Yaseen, Hassan Al-Karagoly","doi":"10.5455/OVJ.2024.v14.i7.14","DOIUrl":"10.5455/OVJ.2024.v14.i7.14","url":null,"abstract":"<p><strong>Background: </strong>Although milk is nutritionally valuable, it also serves as a significant medium for the transmission of pathogens and their toxins.</p><p><strong>Aim: </strong>This study aimed to investigate the role of enterotoxin gene A (SEA) in the development of bovine mastitis. We accomplished this by examining milk through polymerase chain reaction (PCR) testing, amino acid substitution analysis, and phylogenetic analysis.</p><p><strong>Methods: </strong>A total of fifty milk samples were collected from locally bred dairy cows in Al-Diwaniyah, located in southern Iraq. We employed the VITEK-2 platform to validate the diagnosis of <i>Staphylococcus aureus</i> and confirm the results of routine tests (culturing and biochemical tests). Subsequently, the genetic mutation and phylogeny analysis were achieved utilizing DNA sequencing to 16S rRNA and enterotoxin A genes.</p><p><strong>Results: </strong>66% (33/50) of the milk samples found to be contain <i>S. aureus</i> by the VITEK-2 system. Furthermore, 25/33 of the samples were positive by the PCR test. While 60% (15 out of 25) tested positive for the SEA gene. After genomic analysis, we identified amino acid substitutions of serine, glutamine with arginine, tyrosine with cysteine, and aspartic acid with glycine at positions 9, 101, 119, 187, and 191. The phylogenetic investigation demonstrated a genetic relationship between our isolates (Iraqi isolates) and isolates from Indian and the United States.</p><p><strong>Conclusion: </strong>Our study indicated the widespread distribution of the enterotoxin gene A (SEA) of <i>S. aureus</i> among dairy cows. The molecular study revealed significant changes in key amino acids that could play an important role in the bacterium's pathogenesis. The phylogenetic similarities among <i>S. aureus</i> samples from various countries suggest that the bacteria has spread globally.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11338610/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142036516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prophylactic impacts of Lagenaria siceraria against cardiomyopathy induced by doxorubicin in a rat model. 在大鼠模型中,西洋鹅掌楸对多柔比星诱发的心肌病有预防性影响。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-07-01 Epub Date: 2024-07-31 DOI: 10.5455/OVJ.2024.v14.i7.16
Najlaa H Almohmadi, Mona Zahran, Sameh M El-Nabtity, Hazem M Shaheen

Background: Doxorubicin (DOX) is a chemotherapeutic drug applied clinically for the remedy of cancer, but its possibly life-threatening cardiotoxicity effects remain a concern.

Aim: After that, this study evaluates the cardioprotective impacts of Lagenaria siceraria (LSS) oil on DOX induced cardiomyopathy in rats.

Methods: Wistar male rats (n = 28, weighting 190-210 g) were arbitrarily allocated into four equal groups. Group 1 control group (CTR) received normal saline orally (1 ml/kg); group 2 (DOX) received DOX (10 mg/kg); group 3 (DOLS) received DOX + 3 g of Lagenaria siceraria seeds oil/kg; group 4 (LSSO) received LSSO (3 g/kg) daily for 18 days. The serum samples were collected to determine the creatine kinase-MB (CK-MB) isoenzyme, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), and Troponin I activity. At the same time, the catalase, malondialdehyde (MDA), and reduced glutathione (GSH) were assessed in heart tissues. Additionally, histopathological investigations for the heart tissue were performed.

Results: Results revealed no significant change in CK-MB levels between the DOLS group compared to the CTR group (p > 0.05). DOX group confirmed a substantial increase in AST, LDH, and Troponin1 serum levels compared to DOLS and LLSO groups (p < 0.05). The study demonstrated the antioxidant activity of LSS oil against DOX-induced toxicity. The DOX group significantly reduced GSH and catalase levels, with an increase in MDA levels compared to DOLS and LLSO groups. Histopathological analysis showed protective properties of LSS oil against myocardial damage caused by DOX.

Conclusion: This study highlights the favorable impacts of LSS oil in mitigating DOX-triggered cardiotoxicity in a rat model.

背景:多柔比星(Doxorubicin,DOX)是一种用于临床治疗癌症的化疗药物,但其可能危及生命的心脏毒性影响仍是一个令人担忧的问题。目的:本研究评估了西洋参油对 DOX 诱导的大鼠心肌病的心脏保护作用:将 Wistar 雄性大鼠(n = 28,体重 190-210 g)任意分为四组。第 1 组对照组(CTR)口服生理盐水(1 毫升/千克);第 2 组(DOX)口服 DOX(10 毫克/千克);第 3 组(DOLS)口服 DOX + 3 克川贝籽油/千克;第 4 组(LSSO)每天口服川贝籽油(3 克/千克),连续 18 天。采集血清样本以测定肌酸激酶-MB(CK-MB)同工酶、乳酸脱氢酶(LDH)、天门冬氨酸氨基转移酶(AST)和肌钙蛋白 I 的活性。同时,还对心脏组织中的过氧化氢酶、丙二醛(MDA)和还原型谷胱甘肽(GSH)进行了评估。此外,还对心脏组织进行了组织病理学检查:结果显示,DOLS 组与 CTR 组相比,CK-MB 水平无明显变化(P > 0.05)。与 DOLS 组和 LLSO 组相比,DOX 组证实 AST、LDH 和肌钙蛋白 1 血清水平大幅上升(P < 0.05)。该研究证明了 LSS 油对 DOX 引起的毒性具有抗氧化活性。与 DOLS 组和 LLSO 组相比,DOX 组的 GSH 和过氧化氢酶水平明显降低,MDA 水平升高。组织病理学分析表明,LSS 油对 DOX 引起的心肌损伤具有保护作用:本研究强调了 LSS 油在减轻大鼠模型中 DOX 引发的心脏毒性方面的有利影响。
{"title":"Prophylactic impacts of <i>Lagenaria siceraria</i> against cardiomyopathy induced by doxorubicin in a rat model.","authors":"Najlaa H Almohmadi, Mona Zahran, Sameh M El-Nabtity, Hazem M Shaheen","doi":"10.5455/OVJ.2024.v14.i7.16","DOIUrl":"10.5455/OVJ.2024.v14.i7.16","url":null,"abstract":"<p><strong>Background: </strong>Doxorubicin (DOX) is a chemotherapeutic drug applied clinically for the remedy of cancer, but its possibly life-threatening cardiotoxicity effects remain a concern.</p><p><strong>Aim: </strong>After that, this study evaluates the cardioprotective impacts of <i>Lagenaria siceraria</i> (LSS) oil on DOX induced cardiomyopathy in rats.</p><p><strong>Methods: </strong>Wistar male rats (<i>n</i> = 28, weighting 190-210 g) were arbitrarily allocated into four equal groups. Group 1 control group (CTR) received normal saline orally (1 ml/kg); group 2 (DOX) received DOX (10 mg/kg); group 3 (DOLS) received DOX + 3 g of <i>Lagenaria</i> <i>siceraria</i> seeds oil/kg; group 4 (LSSO) received LSSO (3 g/kg) daily for 18 days. The serum samples were collected to determine the creatine kinase-MB (CK-MB) isoenzyme, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), and Troponin I activity. At the same time, the catalase, malondialdehyde (MDA), and reduced glutathione (GSH) were assessed in heart tissues. Additionally, histopathological investigations for the heart tissue were performed.</p><p><strong>Results: </strong>Results revealed no significant change in CK-MB levels between the DOLS group compared to the CTR group (<i>p</i> > 0.05). DOX group confirmed a substantial increase in AST, LDH, and Troponin1 serum levels compared to DOLS and LLSO groups (<i>p</i> < 0.05). The study demonstrated the antioxidant activity of LSS oil against DOX-induced toxicity. The DOX group significantly reduced GSH and catalase levels, with an increase in MDA levels compared to DOLS and LLSO groups. Histopathological analysis showed protective properties of LSS oil against myocardial damage caused by DOX.</p><p><strong>Conclusion: </strong>This study highlights the favorable impacts of LSS oil in mitigating DOX-triggered cardiotoxicity in a rat model.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11338615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142036471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Advantages of Curcuma longa in preventing male infertility caused by thioacetamide. 莪术在预防硫代乙酰胺引起的男性不育症方面的优势。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-07-01 Epub Date: 2024-07-31 DOI: 10.5455/OVJ.2024.v14.i7.8
Fatima A Jaber, Fatima S Alaryani, Boudor S Almutiri, Suzan B Abdu, Arif Mohammed

Background: Thioacetamide (TAA) is known to cause damage to various organs, including the testes, posing a significant health threat. On the other hand, Curcuma longa (Cl) has been recognized for its antioxidant properties, suggesting a potential protective role against TAA-induced toxicity in the testes.

Aim: This study aims to investigate the effect of TAA on testicular function and structure while exploring the therapeutic and protective potential of C. longa versus TAA toxicity.

Methods: Thirty-two male albino rats, with an age range of 11-12 weeks and a weight range of 180-200 g, were randomly allocated into four distinct groups. The control group received normal saline, while the Cl group ingested Cl orally at a dose of 500 mg/kg daily. The TAA group, received TAA through intraperitoneal injections at a dose of 200 mg/kg body weight three times per week. Lastly, the Cl with TAA group received Cl orally 2 hours before the TAA injections. After 8 weeks of treatment, we anesthetized the rats and saved blood samples for biochemical analysis.

Results: The study revealed significant alterations in various biochemical parameters in the TAA-treated group, as compared with the control. Specifically, there was a significant increase in bilirubin, albumin, cholesterol, triglyceride, very low-density lipoprotein, white blood cells, low-density lipoprotein cholesterol, and platelets levels. Conversely, the Cl-treated group exhibited significant reductions in these parameters, along with notable increases in red blood cells, high-density lipoprotein cholesterol, and hemoglobin.

Conclusion: C. longa demonstrates a protective effect on the testes against TAA-induced toxicity, potentially attributed to its antioxidant properties. This suggests a promising avenue for the use of Cl in mitigating the harmful effects of TAA on testicular function and structure.

背景:众所周知,硫代乙酰胺(TAA)会对包括睾丸在内的多个器官造成损害,对健康构成严重威胁。目的:本研究旨在调查硫代乙酰胺对睾丸功能和结构的影响,同时探索莪术对硫代乙酰胺毒性的治疗和保护潜力:方法:将 32 只年龄在 11-12 周、体重在 180-200 克的雄性白化大鼠随机分为四组。对照组接受生理盐水,Cl 组每天口服 500 毫克/千克的 Cl。TAA 组每周三次腹腔注射 TAA,剂量为每公斤体重 200 毫克。最后,Cl与TAA组在注射TAA前2小时口服Cl。治疗 8 周后,我们对大鼠进行麻醉,并保存血液样本用于生化分析:研究发现,与对照组相比,TAA 治疗组大鼠的各种生化指标发生了明显变化。具体而言,胆红素、白蛋白、胆固醇、甘油三酯、极低密度脂蛋白、白细胞、低密度脂蛋白胆固醇和血小板水平均有显著增加。相反,Cl 处理组的这些参数显著降低,红细胞、高密度脂蛋白胆固醇和血红蛋白也明显增加:结论:C. longa 对睾丸具有保护作用,可抵御 TAA 引起的毒性,这可能归功于其抗氧化特性。这为使用 Cl 来减轻 TAA 对睾丸功能和结构的有害影响提供了一条很有前景的途径。
{"title":"Advantages of <i>Curcuma longa</i> in preventing male infertility caused by thioacetamide.","authors":"Fatima A Jaber, Fatima S Alaryani, Boudor S Almutiri, Suzan B Abdu, Arif Mohammed","doi":"10.5455/OVJ.2024.v14.i7.8","DOIUrl":"10.5455/OVJ.2024.v14.i7.8","url":null,"abstract":"<p><strong>Background: </strong>Thioacetamide (TAA) is known to cause damage to various organs, including the testes, posing a significant health threat. On the other hand, <i>Curcuma longa</i> (Cl) has been recognized for its antioxidant properties, suggesting a potential protective role against TAA-induced toxicity in the testes.</p><p><strong>Aim: </strong>This study aims to investigate the effect of TAA on testicular function and structure while exploring the therapeutic and protective potential of <i>C. longa</i> versus TAA toxicity.</p><p><strong>Methods: </strong>Thirty-two male albino rats, with an age range of 11-12 weeks and a weight range of 180-200 g, were randomly allocated into four distinct groups. The control group received normal saline, while the Cl group ingested Cl orally at a dose of 500 mg/kg daily. The TAA group, received TAA through intraperitoneal injections at a dose of 200 mg/kg body weight three times per week. Lastly, the Cl with TAA group received Cl orally 2 hours before the TAA injections. After 8 weeks of treatment, we anesthetized the rats and saved blood samples for biochemical analysis.</p><p><strong>Results: </strong>The study revealed significant alterations in various biochemical parameters in the TAA-treated group, as compared with the control. Specifically, there was a significant increase in bilirubin, albumin, cholesterol, triglyceride, very low-density lipoprotein, white blood cells, low-density lipoprotein cholesterol, and platelets levels. Conversely, the Cl-treated group exhibited significant reductions in these parameters, along with notable increases in red blood cells, high-density lipoprotein cholesterol, and hemoglobin.</p><p><strong>Conclusion: </strong><i>C. longa</i> demonstrates a protective effect on the testes against TAA-induced toxicity, potentially attributed to its antioxidant properties. This suggests a promising avenue for the use of Cl in mitigating the harmful effects of TAA on testicular function and structure.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11338612/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142036543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Acute onset of hypertensive encephalopathy in a dog with right adrenal pheochromocytoma and neoplastic invasion of the caudal vena cava: Case report and review of the literature. 一只患有右肾上腺嗜铬细胞瘤和尾腔静脉肿瘤性侵犯的狗突发高血压脑病:病例报告和文献综述。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-07-01 Epub Date: 2024-07-31 DOI: 10.5455/OVJ.2024.v14.i7.21
Alessandra Recchia, Serena Digiaro, Antonella Colella, Beatrice Greco, Paola Paradies

Background: Canine pheochromocytomas (PCCs) are rare tumors of the adrenal medulla. Clinical signs are often vague, resulting in intermittent catecholamine over secretion or neoplastic invasion of adjacent structures.

Case description: A 12-year-old Epagneul Breton dog with a 1-year history of chronic kidney disease, was examined for acute onset of severe neurological signs. Based on clinical and instrumental data, hypertensive encephalopathy was suspected. Cardiac and abdominal ultrasound were performed. Severe hypertensive cardiopathy and a right adrenal gland mass with invasion of the caudal vena cava were diagnosed. Computed tomography imaging confirmed the suspect of invasive malignant neoplasia. Emergency pharmacological therapy was started to reduce systemic pressure, improve clinical signs, and stabilize the dog in view of surgical resolution. After initial improvement, patient conditions abruptly worsened, and euthanasia was elected. Histology examination confirmed a right adrenal PCC, with caval invasion.

Conclusion: To the authors' conclusions, acute hypertensive encephalopathy is a peculiar manifestation of PCCs. Ultrasound is a useful, and rapid test to suspect PCC as it can detect adrenal alterations, caval invasion, metastasis, and cardiac sequelae consistent with the condition. PCC can mimic multiple affections, and be misinterpreted, especially when a concurrent disease has already been diagnosed. Veterinarians need to be aware that comorbidities could mask clinical signs and delay diagnosis. Furthermore, this clinical case reminds us to include PCC also in the differential diagnosis of dogs with an acute onset of severe neurological signs.

背景:犬嗜铬细胞瘤(PCC)是一种罕见的肾上腺髓质肿瘤。临床症状往往模糊不清,导致间歇性儿茶酚胺分泌过多或肿瘤侵犯邻近结构:病例描述:一只 12 岁的埃帕涅尔布列塔尼犬患有慢性肾病,病史长达 1 年。根据临床和仪器数据,怀疑是高血压脑病。对其进行了心脏和腹部超声波检查。确诊为严重的高血压性心脏病和侵犯尾腔静脉的右肾上腺肿块。计算机断层扫描成像证实了浸润性恶性肿瘤的嫌疑。为了降低全身压力、改善临床症状和稳定病情,以便通过手术解决问题,医生开始对该犬进行紧急药物治疗。最初情况有所改善后,患者病情突然恶化,最终选择了安乐术。组织学检查证实为右肾上腺 PCC,并伴有腔隙侵犯:作者的结论是,急性高血压脑病是 PCC 的一种特殊表现。超声波是怀疑 PCC 的一种有效而快速的检测方法,因为它可以检测出肾上腺改变、腔隙侵犯、转移和心脏后遗症。PCC 可模拟多种疾病并被误诊,尤其是在已经诊断出并发疾病的情况下。兽医需要注意,并发症可能会掩盖临床症状,延误诊断。此外,这个临床病例还提醒我们,在对急性发作的严重神经症状犬进行鉴别诊断时,也应将 PCC 包括在内。
{"title":"Acute onset of hypertensive encephalopathy in a dog with right adrenal pheochromocytoma and neoplastic invasion of the caudal vena cava: Case report and review of the literature.","authors":"Alessandra Recchia, Serena Digiaro, Antonella Colella, Beatrice Greco, Paola Paradies","doi":"10.5455/OVJ.2024.v14.i7.21","DOIUrl":"10.5455/OVJ.2024.v14.i7.21","url":null,"abstract":"<p><strong>Background: </strong>Canine pheochromocytomas (PCCs) are rare tumors of the adrenal medulla. Clinical signs are often vague, resulting in intermittent catecholamine over secretion or neoplastic invasion of adjacent structures.</p><p><strong>Case description: </strong>A 12-year-old Epagneul Breton dog with a 1-year history of chronic kidney disease, was examined for acute onset of severe neurological signs. Based on clinical and instrumental data, hypertensive encephalopathy was suspected. Cardiac and abdominal ultrasound were performed. Severe hypertensive cardiopathy and a right adrenal gland mass with invasion of the caudal vena cava were diagnosed. Computed tomography imaging confirmed the suspect of invasive malignant neoplasia. Emergency pharmacological therapy was started to reduce systemic pressure, improve clinical signs, and stabilize the dog in view of surgical resolution. After initial improvement, patient conditions abruptly worsened, and euthanasia was elected. Histology examination confirmed a right adrenal PCC, with caval invasion.</p><p><strong>Conclusion: </strong>To the authors' conclusions, acute hypertensive encephalopathy is a peculiar manifestation of PCCs. Ultrasound is a useful, and rapid test to suspect PCC as it can detect adrenal alterations, caval invasion, metastasis, and cardiac sequelae consistent with the condition. PCC can mimic multiple affections, and be misinterpreted, especially when a concurrent disease has already been diagnosed. Veterinarians need to be aware that comorbidities could mask clinical signs and delay diagnosis. Furthermore, this clinical case reminds us to include PCC also in the differential diagnosis of dogs with an acute onset of severe neurological signs.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11338619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142036542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Canine primary ureteral leiomyosarcoma treated with unilateral left ureteronephrectomy. 用单侧左输尿管肾切除术治疗犬原发性输尿管亮肌肉瘤。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-07-01 Epub Date: 2024-07-31 DOI: 10.5455/OVJ.2024.v14.i7.20
Danilo C Sousa, Ygor A Rossi, Luíz G D Benevenuto, Carlos E Fonseca-Alves, Larissa F Magalhães, Cynthia M Bueno, Denner S Dos Anjos

Background: Primary ureteral neoplasms are extremely rare in dogs, and ureteral involvement usually occurs owing to the invasion of renal and bladder tumors.

Case description: This case report describes a 12-year-old intact male mixed-breed dog referred to a private clinic with a six-month history of abdominal distention. A physical examination revealed mild abdominal pain. Hematological tests detected normocytic-normochromic anemia (hematocrit 33.6% [reference interval-RI: 37%-55%], red blood cells 4.93 M/µl [RI: 5.5-8.5 M/µl], and hemoglobin 12.4 g/dl [RI: 12-18.0 g/dl]). The results from the leukogram, thrombogram, renal, and hepatic panels were within the reference intervals for dogs. Abdominal ultrasonography revealed a cavitary mass measuring approximately 12 cm in diameter as the largest tumor in the left abdominal region over the left hepatic lobe or mesenteric site. Chest radiography did not reveal any metastasis. Therefore, the patient underwent exploratory laparotomy, during which the left ureter was found to be affected by a 12-cm mass that adhered to the left kidney. A unilateral left ureteronephrectomy was performed, and histology and immunohistochemistry (IHC) confirmed well-differentiated primary ureteral leiomyosarcoma. The patient survived for 130 days but died of lung metastasis.

Conclusion: Ureteral leiomyosarcoma should be investigated and included in the list of differential diagnoses for primary ureteral neoplasms. Regardless of the therapeutic modality, the prognosis of ureteral leiomyosarcoma may be unfavorable, as shown in this report.

背景:原发性输尿管肿瘤在犬中极为罕见,输尿管受累通常是由于肾脏和膀胱肿瘤的侵犯:本病例报告描述的是一只 12 岁的完整雄性混种犬,因腹胀 6 个月的病史而转诊到一家私人诊所。体格检查发现它有轻微腹痛。血液学检查发现该犬患有正常红细胞-正色素性贫血(血细胞比容 33.6% [参考区间:37%-55%],红细胞 4.93 M/µl[参考区间:5.5-8.5 M/µl],血红蛋白 12.4 g/dl [参考区间:12-18.0 g/dl])。白细胞检查、血栓检查、肾脏检查和肝脏检查的结果均在狗的参考范围之内。腹部超声波检查显示,左腹部最大的肿瘤位于左肝叶或肠系膜部位,直径约 12 厘米。胸片检查未发现任何转移。因此,患者接受了探查性开腹手术,术中发现左侧输尿管被一个 12 厘米的肿块影响,肿块与左肾粘连。患者接受了单侧左侧输尿管肾切除术,组织学和免疫组化(IHC)证实为分化良好的原发性输尿管利肌肉瘤。患者存活了130天,但死于肺转移:结论:应该对输尿管利肌瘤进行检查,并将其列入原发性输尿管肿瘤的鉴别诊断清单。正如本报告所示,无论采用哪种治疗方法,输尿管利肌肉瘤的预后都可能不佳。
{"title":"Canine primary ureteral leiomyosarcoma treated with unilateral left ureteronephrectomy.","authors":"Danilo C Sousa, Ygor A Rossi, Luíz G D Benevenuto, Carlos E Fonseca-Alves, Larissa F Magalhães, Cynthia M Bueno, Denner S Dos Anjos","doi":"10.5455/OVJ.2024.v14.i7.20","DOIUrl":"10.5455/OVJ.2024.v14.i7.20","url":null,"abstract":"<p><strong>Background: </strong>Primary ureteral neoplasms are extremely rare in dogs, and ureteral involvement usually occurs owing to the invasion of renal and bladder tumors.</p><p><strong>Case description: </strong>This case report describes a 12-year-old intact male mixed-breed dog referred to a private clinic with a six-month history of abdominal distention. A physical examination revealed mild abdominal pain. Hematological tests detected normocytic-normochromic anemia (hematocrit 33.6% [reference interval-RI: 37%-55%], red blood cells 4.93 M/µl [RI: 5.5-8.5 M/µl], and hemoglobin 12.4 g/dl [RI: 12-18.0 g/dl]). The results from the leukogram, thrombogram, renal, and hepatic panels were within the reference intervals for dogs. Abdominal ultrasonography revealed a cavitary mass measuring approximately 12 cm in diameter as the largest tumor in the left abdominal region over the left hepatic lobe or mesenteric site. Chest radiography did not reveal any metastasis. Therefore, the patient underwent exploratory laparotomy, during which the left ureter was found to be affected by a 12-cm mass that adhered to the left kidney. A unilateral left ureteronephrectomy was performed, and histology and immunohistochemistry (IHC) confirmed well-differentiated primary ureteral leiomyosarcoma. The patient survived for 130 days but died of lung metastasis.</p><p><strong>Conclusion: </strong>Ureteral leiomyosarcoma should be investigated and included in the list of differential diagnoses for primary ureteral neoplasms. Regardless of the therapeutic modality, the prognosis of ureteral leiomyosarcoma may be unfavorable, as shown in this report.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11338613/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142036546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The efficacy of topically applied Sappan wood (Caesalpinia sappan L.) ethanol extract during incision wound healing in albino rats. 在白化大鼠切口伤口愈合过程中局部使用萨班木(Caesalpinia sappan L.)乙醇提取物的疗效。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-07-01 Epub Date: 2024-07-31 DOI: 10.5455/OVJ.2024.v14.i7.5
Ricky Eka Sucita, Faisal Fikri, Agus Purnomo, Salipudin Tasil Maslamama, Muhammad Thohawi Elziyad Purnama

Background: Medical manifestations in the form of incisions, burns, and trauma will trigger a natural wound-healing process that involves complex interactions between cells. Brazilin and other secondary metabolites found in Sappan wood have numerous bioactive qualities, including anti-inflammatory, anti-cancer, and antioxidant properties.

Aim: This study aimed to investigate the efficacy of Sappan wood (Caesalpinia Sappan L.) ethanol extract topically on the incision wound healing of albino rats.

Methods: Twenty male rats were randomly assigned into five groups with four replications, i.e., (C-) was treated ointment-based, (C+) was treated with 10% povidone-iodine, (T1, T2, and T3 groups) were treated with Sappan wood extract concentration for 6.5%, 15%, and 30%, respectively. The treatment was topically administered to wounded areas twice a day for 15 days. Wound healing was evaluated histologically as the following parameters collagen deposition, polymorphonuclear neutrophils (PMN), angiogenesis, and fibrosis degree using H&E staining. IL-2 level was evaluated using the enzyme-linked immunosorbent assay (ELISA) method. Wound length reduction was calculated on days 8 and 15.

Results: As a result, the 6.5% (T1), 15% (T2), and 30% (T3) Sappan wood extract groups were improved significantly (p < 0.05) compared to ointment-based (C-) and povidone-iodine (C+) groups on the collagen deposition, PMN, angiogenesis, fibrosis degree, and IL-2 level. In particular, the 6.5% (T1) Sappan wood extract group was highlighted significantly (p < 0.05) compared to other groups, evidenced by the improvisation of wound healing parameters and reduction of wound length on days 8 and 15.

Conclusion: In conclusion, a 6.5% Sappan wood extract revealed its applicability to improve incision wound healing in albino rats.

背景:切口、烧伤和创伤等医学表现形式会引发自然伤口愈合过程,其中涉及细胞之间复杂的相互作用。目的:本研究旨在探讨皂荚木(Caesalpinia Sappan L.)乙醇提取物局部外用对白化大鼠切口伤口愈合的疗效:将 20 只雄性大鼠随机分为 5 组,每组 4 次重复,即(C- 组)用软膏治疗,(C+ 组)用 10%聚维酮碘治疗,(T1、T2 和 T3 组)用浓度分别为 6.5%、15% 和 30% 的皂荚木提取物治疗。每天两次,连续 15 天。伤口愈合的组织学评估指标包括胶原沉积、多形核中性粒细胞(PMN)、血管生成和纤维化程度(采用 H&E 染色法)。IL-2水平采用酶联免疫吸附试验(ELISA)方法进行评估。在第 8 天和第 15 天计算伤口长度的减少:结果:与软膏组(C-)和聚维酮碘组(C+)相比,6.5%(T1)、15%(T2)和 30%(T3)萨班木提取物组在胶原沉积、PMN、血管生成、纤维化程度和 IL-2 水平方面均有显著改善(P < 0.05)。特别是,与其他组相比,6.5%(T1)山榄仁提取物组在第 8 天和第 15 天伤口愈合参数的改善和伤口长度的缩短方面表现突出(P < 0.05):总之,6.5% 的萨班木提取物显示了其改善白化大鼠切口伤口愈合的适用性。
{"title":"The efficacy of topically applied Sappan wood (<i>Caesalpinia sappan</i> L.) ethanol extract during incision wound healing in albino rats.","authors":"Ricky Eka Sucita, Faisal Fikri, Agus Purnomo, Salipudin Tasil Maslamama, Muhammad Thohawi Elziyad Purnama","doi":"10.5455/OVJ.2024.v14.i7.5","DOIUrl":"10.5455/OVJ.2024.v14.i7.5","url":null,"abstract":"<p><strong>Background: </strong>Medical manifestations in the form of incisions, burns, and trauma will trigger a natural wound-healing process that involves complex interactions between cells. Brazilin and other secondary metabolites found in Sappan wood have numerous bioactive qualities, including anti-inflammatory, anti-cancer, and antioxidant properties.</p><p><strong>Aim: </strong>This study aimed to investigate the efficacy of Sappan wood (<i>Caesalpinia Sappan</i> L.) ethanol extract topically on the incision wound healing of albino rats.</p><p><strong>Methods: </strong>Twenty male rats were randomly assigned into five groups with four replications, i.e., (C-) was treated ointment-based, (C+) was treated with 10% povidone-iodine, (T1, T2, and T3 groups) were treated with Sappan wood extract concentration for 6.5%, 15%, and 30%, respectively. The treatment was topically administered to wounded areas twice a day for 15 days. Wound healing was evaluated histologically as the following parameters collagen deposition, polymorphonuclear neutrophils (PMN), angiogenesis, and fibrosis degree using H&E staining. IL-2 level was evaluated using the enzyme-linked immunosorbent assay (ELISA) method. Wound length reduction was calculated on days 8 and 15.</p><p><strong>Results: </strong>As a result, the 6.5% (T1), 15% (T2), and 30% (T3) Sappan wood extract groups were improved significantly (<i>p</i> < 0.05) compared to ointment-based (C-) and povidone-iodine (C+) groups on the collagen deposition, PMN, angiogenesis, fibrosis degree, and IL-2 level. In particular, the 6.5% (T1) Sappan wood extract group was highlighted significantly (<i>p</i> < 0.05) compared to other groups, evidenced by the improvisation of wound healing parameters and reduction of wound length on days 8 and 15.</p><p><strong>Conclusion: </strong>In conclusion, a 6.5% Sappan wood extract revealed its applicability to improve incision wound healing in albino rats.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11338603/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142036473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of azathioprine on healing of exposed dogs' dental pulp capped with three different materials. 硫唑嘌呤对用三种不同材料覆盖的暴露犬牙髓愈合的影响。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-07-01 Epub Date: 2024-07-31 DOI: 10.5455/OVJ.2024.v14.i7.11
Mokhtar A Al-Anesi, Ashraf M Abu-Seida, Ehab S Abd-Elhamid, Abeer H Mahran, Salma H El Ashry, Mohamed H Issa, Mohamed M Nagy

Background: Azathioprine is one of the earliest immunosuppressants prescribed for several autoimmune diseases. Yet there is a lack of research on the impact of azathioprine on pulp healing following the pulp capping procedure.

Aim: This study aimed to investigate the effect of azathioprine on the healing ability of mechanically exposed dogs' dental pulps following direct pulp capping with mineral trioxide aggregate (MTA), bio-aggregates (BA), and Calcium hydroxide (Ca(OH)2).

Methods: Four mongrel dogs were randomly assigned to two groups (two dogs/30 teeth in each group): immunosuppressed (group I) and control (group II). Group I received azathioprine for two months before surgical treatments and until the dogs were euthanized. Fifteen class V buccal cavities were performed in each dog. Each group was randomly divided into three subgroups (10 teeth each) based on the pulp capping substance. The pulps in subgroups A, B, and C were immediately capped with MTA, BA, and Ca(OH)2, respectively. Inflammation and dentine bridge development were histopathologically evaluated and scored at one and two months. The data were statistically analyzed.

Results: The immunosuppressed group exhibited statistically greater inflammatory cell count and decreased dentine bridge thickness, compared to the control group in all subgroups (p < 0.05).

Conclusion: Azathioprine has an adverse effect on the healing of exposed dogs' dental pulp following direct pulp capping with MTA, BA, and Ca(OH)2. Therefore, patients using azathioprine as an immunosuppressive medication may experience delayed healing of mechanically exposed pulps following capping with MTA, BA, or Ca(OH)2.

背景:硫唑嘌呤是最早用于治疗多种自身免疫性疾病的免疫抑制剂之一。目的:本研究旨在调查硫唑嘌呤对机械暴露犬牙髓的愈合能力的影响,这些牙髓是在使用三氧化物矿物质聚合体(MTA)、生物聚合体(BA)和氢氧化钙(Ca(OH)2)直接覆盖牙髓后愈合的:将四只杂种狗随机分为两组(每组两只狗/30 颗牙齿):免疫抑制组(I 组)和对照组(II 组)。I 组在手术治疗前接受硫唑嘌呤治疗两个月,直至犬只安乐死。每只狗进行了 15 次 V 级颊面针穿刺。根据牙髓覆盖物质的不同,每组随机分为三个亚组(每组 10 颗牙齿)。亚组 A、B 和 C 的牙髓分别立即使用 MTA、BA 和 Ca(OH)2 进行盖髓。炎症和牙本质桥发育情况在一个月和两个月后进行组织病理学评估和评分。对数据进行统计分析:结果:与对照组相比,免疫抑制组在所有亚组中的炎症细胞数量和牙本质桥厚度均有统计学意义的增加(P < 0.05):硫唑嘌呤对暴露犬牙髓在使用 MTA、BA 和 Ca(OH)2 直接盖髓后的愈合有不利影响。因此,使用硫唑嘌呤作为免疫抑制剂的患者在使用 MTA、BA 或 Ca(OH)2 覆膜后,机械暴露牙髓的愈合可能会延迟。
{"title":"Influence of azathioprine on healing of exposed dogs' dental pulp capped with three different materials.","authors":"Mokhtar A Al-Anesi, Ashraf M Abu-Seida, Ehab S Abd-Elhamid, Abeer H Mahran, Salma H El Ashry, Mohamed H Issa, Mohamed M Nagy","doi":"10.5455/OVJ.2024.v14.i7.11","DOIUrl":"10.5455/OVJ.2024.v14.i7.11","url":null,"abstract":"<p><strong>Background: </strong>Azathioprine is one of the earliest immunosuppressants prescribed for several autoimmune diseases. Yet there is a lack of research on the impact of azathioprine on pulp healing following the pulp capping procedure.</p><p><strong>Aim: </strong>This study aimed to investigate the effect of azathioprine on the healing ability of mechanically exposed dogs' dental pulps following direct pulp capping with mineral trioxide aggregate (MTA), bio-aggregates (BA), and Calcium hydroxide (Ca(OH)<sub>2</sub>).</p><p><strong>Methods: </strong>Four mongrel dogs were randomly assigned to two groups (two dogs/30 teeth in each group): immunosuppressed (group I) and control (group II). Group I received azathioprine for two months before surgical treatments and until the dogs were euthanized. Fifteen class V buccal cavities were performed in each dog. Each group was randomly divided into three subgroups (10 teeth each) based on the pulp capping substance. The pulps in subgroups A, B, and C were immediately capped with MTA, BA, and Ca(OH)<sub>2</sub>, respectively. Inflammation and dentine bridge development were histopathologically evaluated and scored at one and two months. The data were statistically analyzed.</p><p><strong>Results: </strong>The immunosuppressed group exhibited statistically greater inflammatory cell count and decreased dentine bridge thickness, compared to the control group in all subgroups (<i>p</i> < 0.05).</p><p><strong>Conclusion: </strong>Azathioprine has an adverse effect on the healing of exposed dogs' dental pulp following direct pulp capping with MTA, BA, and Ca(OH)<sub>2</sub>. Therefore, patients using azathioprine as an immunosuppressive medication may experience delayed healing of mechanically exposed pulps following capping with MTA, BA, or Ca(OH)<sub>2</sub>.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11338621/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142036549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Animal welfare during transport, evolution, and perspectives of European Union Legislation and Policy. 运输过程中的动物福利、演变以及欧盟立法和政策的前景。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-07-01 Epub Date: 2024-07-31 DOI: 10.5455/OVJ.2024.v14.i7.1
Massimo Giangaspero, Pasquale Turno

In the one health approach, which summarizes the concept that human, animal, and plant health are interdependent and linked to the ecosystems in which they exist, animal welfare assumes an important role. In addition, the Farm to Fork Strategy of the European Union recognizes animal welfare as an essential component. Transport of animals involves a number of issues related to welfare. Animals are subjected to continuous stress, not only during transit on vehicles, vessels, trains, or airplanes, but also during loading and unloading operations. The handling and transport conditions have improved from basic and relatively rudimentary systems, with elements to attenuate the discomfort of animals having been gradually introduced, especially for long-distance transport. Despite several improvements in animal welfare standards during transportation there are still pressing issues in current EU legislation which deserve reviewing. Recently, several scientific opinions on animal welfare, including during transport, have been published by the European Food Safety Agency, following requests by the European Commission. In addition, the European Court of Auditors published its last report focused on animal welfare during transport. These reports underline the need to review current legislation governing the topic and highlight a number of outstanding issues in the European Union and in Third Countries. This article summarizes the history and current status of this complex issue.

在 "一体健康 "方法中,动物福利扮演着重要角色。"一体健康 "方法概括了人类、动物和植物的健康相互依存并与其所处的生态系统相关联的概念。此外,欧盟的 "从农场到餐桌 "战略也将动物福利视为一个重要组成部分。动物运输涉及许多与动物福利相关的问题。动物不仅在汽车、轮船、火车或飞机的运输过程中,而且在装卸过程中都会持续受到压力。装卸和运输条件已从基本和相对简陋的系统中得到改善,特别是在长途运输中,逐渐引入了减轻动物不适的元素。尽管运输过程中的动物福利标准有了一些改善,但欧盟现行立法中仍有一些亟待解决的问题值得审查。最近,在欧盟委员会的要求下,欧洲食品安全局公布了一些关于动物福利(包括运输过程中的动物福利)的科学意见。此外,欧洲审计法院发布了最后一份报告,重点关注运输过程中的动物福利问题。这些报告强调了审查有关该主题的现行立法的必要性,并着重指出了欧盟和第三国的一些未决问题。本文概述了这一复杂问题的历史和现状。
{"title":"Animal welfare during transport, evolution, and perspectives of European Union Legislation and Policy.","authors":"Massimo Giangaspero, Pasquale Turno","doi":"10.5455/OVJ.2024.v14.i7.1","DOIUrl":"10.5455/OVJ.2024.v14.i7.1","url":null,"abstract":"<p><p>In the one health approach, which summarizes the concept that human, animal, and plant health are interdependent and linked to the ecosystems in which they exist, animal welfare assumes an important role. In addition, the Farm to Fork Strategy of the European Union recognizes animal welfare as an essential component. Transport of animals involves a number of issues related to welfare. Animals are subjected to continuous stress, not only during transit on vehicles, vessels, trains, or airplanes, but also during loading and unloading operations. The handling and transport conditions have improved from basic and relatively rudimentary systems, with elements to attenuate the discomfort of animals having been gradually introduced, especially for long-distance transport. Despite several improvements in animal welfare standards during transportation there are still pressing issues in current EU legislation which deserve reviewing. Recently, several scientific opinions on animal welfare, including during transport, have been published by the European Food Safety Agency, following requests by the European Commission. In addition, the European Court of Auditors published its last report focused on animal welfare during transport. These reports underline the need to review current legislation governing the topic and highlight a number of outstanding issues in the European Union and in Third Countries. This article summarizes the history and current status of this complex issue.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11338620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142036544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Open Veterinary Journal
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1