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Monitoring of bone matrix acidification by TRAP and ERK biomarkers in the chronic hypercholesterolemia male rats. 通过 TRAP 和 ERK 生物标记监测慢性高胆固醇血症雄性大鼠骨基质的酸化。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI: 10.5455/OVJ.2024.v14.i8.11
Duaa Raad Abd-Al-Ameer, Wefak Albazi, Hayder Ali Muhammed

Background: Hypercholesterolemia is frequently linked to an elevated risk of cardiovascular diseases, including heart attacks and strokes. Additionally, it could be connected to a higher susceptibility to osteoporosis. Hypercholesterolemia can stimulate the differentiation and activity of osteoclasts, leading to enhanced bone reabsorption and a subsequent net loss of bone tissue.

Aim: The purpose of this study was to examine the influence of a high-cholesterol diet on osteoporosis in male rats with differences in biological and oxidative indicators in the hypercholesterolemia diet in male rats.

Methods: The samples in this study were twenty male rats, ranging between 1.5 and 2 months, were separated into two groups. In one group, 10 rats were fed a regular diet, while in another group, 10 rats were fed a high-cholesterol diet (2%) over the course of 8 weeks. Samples of blood were obtained at the last stage of the experiment. To calculate physiological and biological markers including extracellular signal-regulated kinase (ERK), tartrate-resistant acid phosphatase (TRAP), hormones, malondialdehyde (MDA), and glutathione (GSH).

Results: The results of this study demonstrated a decrease in GSH levels, an increase in ERKs, no significant change in serum TRAP levels, an increase in MDA levels in the blood, and elevated levels of parathyroid hormone, calcitonin, and vitamin D in the cholesterol group.

Conclusion: Increased oxidative stress, altered signaling, and disruptions in calcium/bone metabolism associated with cholesterol-related conditions and monitoring biomarker ERK can provide valuable information about disease progression.

背景:高胆固醇血症经常与心血管疾病(包括心脏病发作和中风)的风险升高有关。此外,它还可能与骨质疏松症的高易感性有关。高胆固醇血症可刺激破骨细胞的分化和活性,导致骨吸收增强,进而造成骨组织的净损失。目的:本研究旨在探讨高胆固醇饮食对雄性大鼠骨质疏松症的影响,以及高胆固醇血症饮食对雄性大鼠生物和氧化指标的影响:本研究的样本为 20 只雄性大鼠,年龄在 1.5 个月到 2 个月之间,分为两组。一组中的 10 只大鼠摄入普通饮食,另一组中的 10 只大鼠摄入高胆固醇饮食(2%),为期 8 周。在实验的最后阶段采集血液样本。计算生理和生物指标,包括细胞外信号调节激酶(ERK)、耐酒石酸磷酸酶(TRAP)、激素、丙二醛(MDA)和谷胱甘肽(GSH):研究结果表明,胆固醇组的 GSH 水平降低,ERKs 水平升高,血清 TRAP 水平无明显变化,血液中 MDA 水平升高,甲状旁腺激素、降钙素和维生素 D 水平升高:结论:氧化应激增加、信号传导改变、钙/骨代谢紊乱与胆固醇相关疾病有关,监测生物标志物ERK可为疾病进展提供有价值的信息。
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引用次数: 0
Renal abscess in a Lionhead rabbit due to Encephalitozoon cuniculi and Escherichia coli: A case report. 狮头兔的肾脓肿是由阴沟肠杆菌和大肠杆菌引起的:病例报告。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI: 10.5455/OVJ.2024.v14.i8.38
Warisraporn Tangchang, Sang-Hun Kim, Su-Young Park, Eun-Hye Jung, Hyo-Jung Kwon, Hwa-Young Son

Background: In rabbits, renal abscesses (pus-filled sores) are rare and diagnosis remains challenging. Therefore, in this study, we aimed to determine the clinical manifestation and diagnostic tests associated with renal abscess identification in rabbits.

Case description: A four-and-a-half-year-old castrated male Lionhead rabbit with a history of poor appetite and abdominal distension was admitted to the animal hospital. Blood analysis, radiography, ultrasonography, and computed tomography scans revealed a kidney abscess found within the renal parenchyma, with severe loss of the cortex and medulla, extending toward the capsule. Consequently, the rabbit underwent nephrectomy. The enlarged right kidney was surgically removed. Histopathological examination of the affected kidney showed severe necrosis and ischemic zones, atrophy of the renal tubules, and prominent heterophils with mixed inflammatory cell infiltrates. Immunohistochemistry and polymerase chain reaction confirmed Encephalitozoon cuniculi and Escherichia coli infections, respectively.

Conclusion: This report provides novel insights into the diagnosis of renal abscesses in Lionhead rabbits.

背景:兔肾脓肿(充满脓液的溃疡)非常罕见,诊断仍具有挑战性。因此,在本研究中,我们旨在确定兔子肾脓肿的临床表现和与肾脓肿鉴别相关的诊断测试:动物医院收治了一只四岁半的阉割雄性狮头兔,该兔有食欲不振和腹胀病史。血液分析、放射线检查、超声波检查和计算机断层扫描显示,在肾实质内发现了肾脓肿,皮质和髓质严重脱落,并向囊内延伸。因此,兔子接受了肾切除手术。手术切除了肿大的右肾。受影响肾脏的组织病理学检查显示出严重的坏死和缺血区、肾小管萎缩以及明显的异嗜酸性粒细胞和混合炎症细胞浸润。免疫组化和聚合酶链反应分别证实感染了阴沟脑虫和大肠杆菌:本报告为诊断狮头兔肾脓肿提供了新见解。
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引用次数: 0
Sudden death due to enterotoxemia among Arabian camels (Camelus dromedaries) and associated risk factors. 阿拉伯骆驼(Camelus dromedaries)肠毒血症导致的猝死及相关风险因素。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI: 10.5455/OVJ.2024.v14.i8.23
Asmaa G Mubarak, Fatma A Khalifa, Yumna Elsobky, Ahmed Abdel-Rady, Wael Felefel, Adel Hassan Saad, Ehab Y Abdelhiee, Abdullah M Alhassan, Hisham Awny, Eman M Elghazaly, Ashraf M Abu-Seida, Abdulrahman Abdulkarim, Asmaa G Youseef

Background: Sudden death is defined as an unexpected death occurring with no observed antecedent clinical signs.

Aim: The current study was performed to notice the tangible causes of sudden death among 51 out of 340 she-camels on a private farm in the eastern region of El Khafgi, Saudi Arabia.

Methods: A retrospective cohort study design was conducted to investigate the sudden death of camels through microscopic examination of fecal matter to identify the gastrointestinal parasites, analysis of whole blood thin films to diagnose blood parasites, blood culturing to recognize bacterial infection as Pasteurella multicida, and macroscopic postmortem examination to identify the gastrointestinal adult worm. The quantity and composition of feed were also analyzed. Afterward, a commercial multiscreen Ag-ELISA kit technique determined the toxins of Clostridium perfringens (C. perfringens).

Results: The results revealed that the incidence rate of sudden death was 15%. The sudden death occurred due to C. perfringens enterotoxins detected in the rumen, intestinal content, and intestinal wall. The enterotoxins and Alpha toxins were noticed, but the other toxin types, including Beta and Epsilon, could not be detected. All C. perfringens toxins were discovered to be negative in fecal matter. A significant association was reported between sudden death, she-camels age, and feeding habits as risk factors (p = 0.020 and 0.028, respectively). Risk factor assessment by relative risk (RR) revealed that the odds of RR of sudden death occurring among she-camels aged over two years were higher than those less than two years (2.24 CI 95%, 1.093-4.591). Furthermore, the odds RR of sudden death occurring due to exposure of she-camels to a concentrated ration of 18% were higher twice than those not exposed (2.346 CI 95%, 1.039-5.296).

Conclusion: Clostridium perfringens enterotoxaemia should be listed as a cause of sudden death in camels and the alteration in diet with 18% concentration feed changes the intestinal environment, which leads to C. perfringens proliferating and yielding potent toxins. More observations and interferences like regular immunization are recommended to reduce the disease and increase the awareness of the farmers of the importance of risk factors.

背景:猝死的定义是在没有观察到先兆临床症状的情况下发生的意外死亡。目的:本研究旨在了解沙特阿拉伯哈夫吉东部地区一家私人农场 340 头母骆驼中 51 头骆驼猝死的具体原因:采用回顾性队列研究设计,通过粪便显微镜检查确定胃肠道寄生虫、全血薄片分析诊断血液寄生虫、血液培养确定细菌感染为多杀性巴氏杆菌,以及尸体宏观检查确定胃肠道成虫,对骆驼猝死进行调查。此外,还对饲料的数量和成分进行了分析。随后,采用商用多筛Ag-ELISA试剂盒技术测定了产气荚膜梭菌(C. perfringens)的毒素:结果显示,猝死发生率为 15%。猝死发生的原因是在瘤胃、肠内容物和肠壁中检测到了产气荚膜梭菌肠毒素。发现了肠毒素和α毒素,但未检测到其他毒素类型,包括β和ε毒素。在粪便中发现的所有产气荚膜杆菌毒素均为阴性。据报告,猝死、母驼年龄和饲养习惯作为风险因素之间存在明显关联(p = 0.020 和 0.028)。通过相对风险(RR)对风险因素进行评估后发现,两岁以上母羚羊发生猝死的几率比两岁以下母羚羊高(2.24 CI 95%,1.093-4.591)。此外,雌驼鸟因接触 18% 的浓缩配给而发生猝死的几率比率是未接触者的两倍(2.346 CI 95%,1.039-5.296):应将产气荚膜梭菌肠毒血症列为骆驼猝死的原因之一,18% 浓度饲料改变了日粮,改变了肠道环境,导致产气荚膜梭菌增殖并产生强效毒素。建议进行更多的观察和干预,如定期免疫接种,以减少疾病的发生,并提高农民对风险因素重要性的认识。
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引用次数: 0
Ultrasonographic evaluation of thoracic and abdominal neoplasia in domestic ruminants: A systemic review. 家养反刍动物胸部和腹部肿瘤的超声评估:系统回顾。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI: 10.5455/OVJ.2024.v14.i8.2
Mohamed Tharwat, El-Sayed El-Shafaey, Abdulrahman A Alkheraif

There is a lack of literature on the usefulness of ultrasonography in the diagnosis and prognosis of thoracic and abdominal neoplasia in domestic ruminants, such as cattle, camels, sheep, and goats. This review aims to shed light on the current applications and prospects of ultrasonography in the diagnosis of thoracic and abdominal neoplasia in domestic ruminants. The scientific literature on ultrasonographic evaluation of the thoracic and abdominal neoplasia in domestic ruminants has been systematically reviewed to verify the potential role of ultrasonography in diagnosing such neoplasia. Based on the literature results, cattle (71.03%) were the most affected animals by thoracic and abdominal neoplasia, followed by sheep (11.86%), goats (11.57%), and camels (5.54%). In all included species, the abdominal neoplasia was more frequent (6.18%) than the thoracic neoplasia (2.97%), and the most frequent neoplasms were forestomach neoplasia. It is concluded that ultrasonography is not widely used in diagnosing thoracic and abdominal neoplasia in ruminant practice. Using ultrasonography in conjunction with guided needle aspiration and biopsy can offer veterinarians the opportunity for more precise diagnosis and treatment decisions guidance of thoracic and abdominal neoplasia in domesticated ruminants.

关于超声波造影在家养反刍动物(如牛、骆驼、绵羊和山羊)胸腔和腹腔肿瘤的诊断和预后中的作用,目前还缺乏相关文献。本综述旨在阐明超声波检查在诊断家养反刍动物胸部和腹部肿瘤方面的应用现状和前景。我们系统回顾了有关家养反刍动物胸腔和腹腔肿瘤超声波评估的科学文献,以验证超声波在诊断此类肿瘤方面的潜在作用。根据文献结果,牛(71.03%)是受胸腔和腹腔肿瘤影响最大的动物,其次是绵羊(11.86%)、山羊(11.57%)和骆驼(5.54%)。在所有纳入的物种中,腹部肿瘤(6.18%)的发病率高于胸部肿瘤(2.97%),最常见的肿瘤是森林胃肿瘤。结论是,在反刍动物实践中,超声波检查在诊断胸腔和腹腔肿瘤方面的应用并不广泛。使用超声波与引导针吸术和活组织检查相结合,可为兽医提供更精确的诊断机会,并为驯养反刍动物胸腔和腹腔肿瘤的治疗决策提供指导。
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引用次数: 0
Bovine umbilical mesenchymal stem cell-conditioned medium increased expression of GLUT-4 in pancreas of diabetic rats induced by nicotinamide-streptozotocin. 牛脐间充质干细胞调节培养基可增加烟酰胺-链脲佐菌素诱导的糖尿病大鼠胰腺中 GLUT-4 的表达。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI: 10.5455/OVJ.2024.v14.i8.3
Herawati Herawati, Hevi Wihadmadyatami, Muhammad Ali Zulfikar, Ricadonna Raissa, Made Bagus Auriva Mataram, Siti Kurniawati, Dyah Ayu O A Pratama

Background: Diabetes is a degenerative disease associated with metabolic disorders. The majority of people have type 2 diabetes mellitus (DM) insulin resistance due to an unhealthy lifestyle. The development of DM treatment is also growing, one of which is using conditioned medium.

Aim: This study aims to determine the effect of Bovine umbilical mesenchymal stem cell-conditioned medium (BUMSC-CM) on nicotinamide (NA) and streptozotocin (STZ) induced rats as an animal model of DM.

Methods: The study began with the in vitro docking of Cholecalciferol with aldolase reductase and glucokinase. In the in vivo study, animal models were divided into five groups: group A (negative control), group B (diabetic rats), group C (NA+STZ+Metformin), group D (NA+STZ+ BUMSC-CM 0.2 ml/kg BW), and group E (NA+STZ+ BUMSC-CM 0.5 ml/kg BW). Blood sugar levels were checked, and BUMSC-CM was administered by intramuscular injection at four-day intervals for a duration of 16 days. Blood sugar levels were also sampled, and GLUT4 histochemical and immunohistochemical staining was performed.

Results: The results showed that Cholecalciferol can bind to aldolase reductase ASP43 and TYR48 and bind to glucokinase at TYR214 with hydrogen bonds. BUMSC-CM administration was able to reduce blood sugar well. In addition, BUMSC-CM also helped repair the tissue structure of the pancreas damaged by inflammation from STZ administration.

Conclusion: This study can be concluded that the administration of BUMSC-CM can be an alternative cell-free therapy for patients with DM.

背景:糖尿病是一种与代谢紊乱有关的退化性疾病。由于不健康的生活方式,大多数人患有 2 型糖尿病(DM)胰岛素抵抗。目的:本研究旨在确定牛脐间充质干细胞条件培养基(BUMSC-CM)对烟酰胺(NA)和链脲佐菌素(STZ)诱导的糖尿病动物模型大鼠的影响:研究从胆钙醇与醛缩酶和葡萄糖激酶的体外对接开始。在体内研究中,动物模型被分为五组:A 组(阴性对照)、B 组(糖尿病大鼠)、C 组(NA+STZ+二甲双胍)、D 组(NA+STZ+ BUMSC-CM 0.2 ml/kg BW)和 E 组(NA+STZ+ BUMSC-CM 0.5 ml/kg BW)。检查血糖水平,每隔四天肌肉注射一次 BUMSC-CM,持续 16 天。同时对血糖水平进行采样,并进行 GLUT4 组织化学和免疫组化染色:结果表明,胆钙化醇能与醛缩酶还原酶 ASP43 和 TYR48 结合,并通过氢键与葡萄糖激酶 TYR214 结合。服用 BUMSC-CM 能够很好地降低血糖。此外,BUMSC-CM 还有助于修复因 STZ 而发炎受损的胰腺组织结构:本研究的结论是,服用 BUMSC-CM 可作为 DM 患者的一种替代性无细胞疗法。
{"title":"Bovine umbilical mesenchymal stem cell-conditioned medium increased expression of GLUT-4 in pancreas of diabetic rats induced by nicotinamide-streptozotocin.","authors":"Herawati Herawati, Hevi Wihadmadyatami, Muhammad Ali Zulfikar, Ricadonna Raissa, Made Bagus Auriva Mataram, Siti Kurniawati, Dyah Ayu O A Pratama","doi":"10.5455/OVJ.2024.v14.i8.3","DOIUrl":"10.5455/OVJ.2024.v14.i8.3","url":null,"abstract":"<p><strong>Background: </strong>Diabetes is a degenerative disease associated with metabolic disorders. The majority of people have type 2 diabetes mellitus (DM) insulin resistance due to an unhealthy lifestyle. The development of DM treatment is also growing, one of which is using conditioned medium.</p><p><strong>Aim: </strong>This study aims to determine the effect of Bovine umbilical mesenchymal stem cell-conditioned medium (BUMSC-CM) on nicotinamide (NA) and streptozotocin (STZ) induced rats as an animal model of DM.</p><p><strong>Methods: </strong>The study began with the <i>in vitro</i> docking of Cholecalciferol with aldolase reductase and glucokinase. In the <i>in vivo</i> study, animal models were divided into five groups: group A (negative control), group B (diabetic rats), group C (NA+STZ+Metformin), group D (NA+STZ+ BUMSC-CM 0.2 ml/kg BW), and group E (NA+STZ+ BUMSC-CM 0.5 ml/kg BW). Blood sugar levels were checked, and BUMSC-CM was administered by intramuscular injection at four-day intervals for a duration of 16 days. Blood sugar levels were also sampled, and GLUT4 histochemical and immunohistochemical staining was performed.</p><p><strong>Results: </strong>The results showed that Cholecalciferol can bind to aldolase reductase ASP43 and TYR48 and bind to glucokinase at TYR214 with hydrogen bonds. BUMSC-CM administration was able to reduce blood sugar well. In addition, BUMSC-CM also helped repair the tissue structure of the pancreas damaged by inflammation from STZ administration.</p><p><strong>Conclusion: </strong>This study can be concluded that the administration of BUMSC-CM can be an alternative cell-free therapy for patients with DM.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Ginkgo biloba administration to apoptosis in rat (Rattus novergicus) brain cells exposed to Plumbum. 银杏叶对大鼠(Rattus novergicus)脑细胞凋亡的影响。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI: 10.5455/OVJ.2024.v14.i8.34
Muhammad Hamdan, Widjiati Widjiati, Priya Nugraha, Jovian Philip Swatan

Background: Lead (Plumbum/Pb) has been identified as a potential cause of Parkinson's disease as well as possibly contributing to disease progression. Ginkgo biloba extract has been widely used to prevent and treat stroke which prevents brain cell apoptosis and neuroinflammation. This has been shown to be beneficial in cognitive recovery in stroke incidents.

Aim: This study aimed to determine the effect of G. biloba on the expression of superoxide dismutase (SOD), malondialdehyde (MDA), and apoptosis-inducing factor (AIF) in the brain cells of rats (Rattus novergicus) exposed to Plumbum.

Methods: The experimental animals used were 36 male white rats divided into 4 groups with different treatments of Plumbum and G. biloba at varying doses for 42 days, after which the brains were collected for examination of SOD, MDA, and AIF expressions using immunohistochemical methods and analyzed using analysis of variance and Duncan's test.

Results: Plumbum administration caused a significant decrease in SOD expression and an increase in MDA and AIF expressions (p < 0.05). Ginkgo biloba administration significantly increased SOD expression and decreased MDA and AIF expressions (p < 0.05), with optimal increases in SOD, decreases in MDA, and modulation of AIF observed in the group exposed to 50 mg/kg BW Pb and 300 mg/kg BW G. biloba.

Conclusion: Preventive administration of G biloba increased SOD expression, and reduced MDA and AIF expressions in Pb-exposed rats, with an optimal dose of 300 mg/kg BW, suggesting its potential as an affordable drug to prevent brain cell death-related diseases.

背景:铅(Plumbum/Pb)已被确定为帕金森病的潜在病因,并可能导致疾病进展。银杏叶提取物被广泛用于预防和治疗中风,它能防止脑细胞凋亡和神经炎症。目的:本研究旨在确定银杏叶提取物对大鼠(Rattus novergicus)脑细胞中超氧化物歧化酶(SOD)、丙二醛(MDA)和凋亡诱导因子(AIF)表达的影响:实验动物为36只雄性白鼠,分为4组,分别服用不同剂量的板蓝根和双叶皂苷42天,然后收集大脑,用免疫组化方法检测SOD、MDA和AIF的表达,并用方差分析和邓肯检验进行分析:结果:服用板蓝根会导致 SOD 表达显著下降,MDA 和 AIF 表达上升(p < 0.05)。银杏叶能显著增加 SOD 的表达,降低 MDA 和 AIF 的表达(p < 0.05),在暴露于 50 mg/kg BW 铅和 300 mg/kg BW 银杏叶的组中,SOD 的增加、MDA 的降低和 AIF 的调节均达到最佳水平:结论:在暴露于铅的大鼠中,预防性服用银杏叶可增加 SOD 的表达,降低 MDA 和 AIF 的表达,最佳剂量为 300 毫克/千克体重。
{"title":"Effect of <i>Ginkgo biloba</i> administration to apoptosis in rat (<i>Rattus novergicus</i>) brain cells exposed to Plumbum.","authors":"Muhammad Hamdan, Widjiati Widjiati, Priya Nugraha, Jovian Philip Swatan","doi":"10.5455/OVJ.2024.v14.i8.34","DOIUrl":"10.5455/OVJ.2024.v14.i8.34","url":null,"abstract":"<p><strong>Background: </strong>Lead (Plumbum/Pb) has been identified as a potential cause of Parkinson's disease as well as possibly contributing to disease progression. <i>Ginkgo biloba</i> extract has been widely used to prevent and treat stroke which prevents brain cell apoptosis and neuroinflammation. This has been shown to be beneficial in cognitive recovery in stroke incidents.</p><p><strong>Aim: </strong>This study aimed to determine the effect of <i>G. biloba</i> on the expression of superoxide dismutase (SOD), malondialdehyde (MDA), and apoptosis-inducing factor (AIF) in the brain cells of rats (<i>Rattus novergicus</i>) exposed to Plumbum.</p><p><strong>Methods: </strong>The experimental animals used were 36 male white rats divided into 4 groups with different treatments of Plumbum and <i>G. biloba</i> at varying doses for 42 days, after which the brains were collected for examination of SOD, MDA, and AIF expressions using immunohistochemical methods and analyzed using analysis of variance and Duncan's test.</p><p><strong>Results: </strong>Plumbum administration caused a significant decrease in SOD expression and an increase in MDA and AIF expressions (<i>p</i> < 0.05). <i>Ginkgo biloba</i> administration significantly increased SOD expression and decreased MDA and AIF expressions (<i>p</i> < 0.05), with optimal increases in SOD, decreases in MDA, and modulation of AIF observed in the group exposed to 50 mg/kg BW Pb and 300 mg/kg BW <i>G. biloba</i>.</p><p><strong>Conclusion: </strong>Preventive administration of <i>G biloba</i> increased SOD expression, and reduced MDA and AIF expressions in Pb-exposed rats, with an optimal dose of 300 mg/kg BW, suggesting its potential as an affordable drug to prevent brain cell death-related diseases.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of GnRH treatment as a potential solution for ovarian disorders in dairy cows infected with foot and mouth disease in Indonesian smallholder farms. GnRH 治疗对印度尼西亚小农农场中感染口蹄疫的奶牛卵巢功能紊乱的潜在解决方案的影响。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI: 10.5455/OVJ.2024.v14.i8.37
Joko Susilo, Erif Maha Nugraha Setyawan, Slamet Hartanto, Michael Haryadi Wibowo, Agung Budiyanto

Background: The outbreak of foot and mouth disease (FMD) in Indonesia induces reproductive disorders in dairy cows that lead to economic losses to smallholder dairy farms.

Aim: The study was to assess the influence of FMD on reproductive traits and evaluate the effect of gonadotropin hormone-releasing hormone (GnRH) administrations on the reproductive performance in FMD-infected dairy cows.

Methods: The study was conducted in Jemowo village, Taman Sari sub-district, Boyolali district, Central Java, Indonesia. A total of 155 cows were used to identify the reproductive disorders on FMD-infected dairy cows aged 2-10 years old. Cows were raised in similar conditions and fed diets. A single dose of 2 ml GnRH was injected intramuscularly into 96 ovarian disorder cows. Reproductive performance was measured by service per conception (S/C), conception rate (CR), and pregnancy rate (PR). A descriptive study was conducted to demonstrate the results.

Results: The study showed that 61.9% of FMD-infected cows had reproductive disorders, whereby 53.5% ovarian hypofunction, 4.52% silent heat, 1.94% repeat breeder, 1.29% ovarian atrophy, and 0.65% endometritis. FMD-infected cows injected with GnRH had a 98% reproductive recovery rate. Moreover, the S/C, CR, and PR of cows injected with GnRH were 2.02%, 51%, and 85%.

Conclusion: GnRH administrations enhanced the reproductive traits of FMD-infected dairy cows indicated by the improvement of CR and PR.

背景:目的:本研究旨在评估口蹄疫对奶牛繁殖性状的影响,并评估促性腺激素释放激素(GnRH)对口蹄疫感染奶牛繁殖性能的影响:研究在印度尼西亚中爪哇省 Boyolali 县 Taman Sari 分区的 Jemowo 村进行。共使用了 155 头奶牛来鉴定 2-10 岁感染口蹄疫的奶牛的繁殖障碍。奶牛的饲养条件和日粮相似。对 96 头卵巢失调奶牛肌肉注射单剂量 2 毫升 GnRH。繁殖性能通过每次受孕次数(S/C)、受孕率(CR)和妊娠率(PR)来衡量。研究采用描述性研究的方法来展示结果:研究表明,61.9% 的 FMD 感染奶牛存在繁殖障碍,其中卵巢功能低下占 53.5%,静默发情占 4.52%,重复配种占 1.94%,卵巢萎缩占 1.29%,子宫内膜炎占 0.65%。注射 GnRH 的 FMD 感染奶牛的繁殖恢复率为 98%。此外,注射 GnRH 的奶牛的 S/C、CR 和 PR 分别为 2.02%、51% 和 85%:结论:注射 GnRH 能提高感染 FMD 的奶牛的繁殖性能,表现在 CR 和 PR 的改善上。
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引用次数: 0
Metazoan ectoparasites of Lithognathus mormyrus (Linnaeus, 1758) from the western coast of Libya. 利比亚西海岸 Lithognathus mormyrus (Linnaeus, 1758) 的寄生虫。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI: 10.5455/OVJ.2024.v14.i8.4
Malak T Altikbali, Mohamed L Showehdi, Sarah A Benzeglam, Aisha A Seif-Alnaser, Esmail A Shakman

Background: Lithognathus mormyrus fish is one of the fish of high economic importance in the countries of the world in general and the Mediterranean countries in specific, including the Libyan Sea waters, for this it is necessary to know everything related to this type of fish, including its infection with parasites. Parasites are considered one of the factors threatening the life of fish, being one of the food chains and the ecological composition of life.

Aim: The current study aims to isolate and identify the parasites that infect the L. mormyrus.

Methods: A total of 368 specimens of L. mormyrus were collected by fishermen, from the western coast of Libya. The study was focused on metazoan parasites. When fish were fully examined for the presence of ectoparasites under a dissecting microscope with incident light, the software camera connected with a microscope and semichon' acetocarmine technique for identification of parasites.

Results: Two species of Monogenea (Encotyllabe valley, Pagellicotyle mormyri, and Lamellodiscus spp.), Isopoda (Gnathia), Copepoda (Lernaeolophus sultanus), and Annelidae (Trachelobdella lubrica) has been isolated from this species of fish. The highest prevalence of infection was Gnathia parasites (8.47%).

Conclusion: There were differences in the parasite species that infect L. mormyrus from one country to another, and also from city to other cities in the same country, as in the Tunisian waters.

背景:Lithognathus mormyrus 鱼是世界各国,特别是地中海国家(包括利比亚海域)具有高度经济重要性的鱼类之一,因此有必要了解与此类鱼类有关的一切,包括其感染寄生虫的情况。寄生虫被认为是威胁鱼类生命的因素之一,是食物链和生命生态组成的一部分:方法:渔民从利比亚西海岸共采集了 368 个鲂鱼标本。研究重点是元虫寄生虫。用入射光在解剖显微镜下对鱼类进行全面检查,以确定是否存在体外寄生虫:结果:从该鱼种中分离出两种单口纲寄生虫(Encotyllabe valley、Pagellicotyle mormyri 和 Lamellodiscus spp.)、等足目寄生虫(Gnathia)、桡足目寄生虫(Lernaeolophus sultanus)和鞘翅目寄生虫(Trachelobdella lubrica)。结论:寄生虫种类之间存在差异:结论:在突尼斯水域,不同国家、同一国家的不同城市感染的寄生虫种类存在差异。
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引用次数: 0
Quality and kinematic characteristics of Bali bulls frozen semen with purified green tea extract epigallocatechin-3-gallate antioxidant addition in diluent. 在稀释剂中添加纯化绿茶提取物表没食子儿茶素-3-棓酸盐抗氧化剂的巴厘公牛冷冻精液的质量和运动特性。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI: 10.5455/OVJ.2024.v14.i8.33
Ragil Angga Prastiya, Trilas Sardjito, Amung Logam Saputro, Darmawan Setia Budi, Styuderil Imaniro Maxdhameta, Elis Sulistiyawati, Deny Sulistyowati, Anny Amaliya, Samira Musa Sasi, And Nining Haryuni

Background: The preservation of semen quality and kinematic characteristics during cryopreservation is crucial for the reproductive success and genetic management of livestock, particularly in Bali bulls.

Aim: This study aimed to investigate the effect of adding purified green tea extract antioxidant Epigallocatechin-3-gallate (EGCG) in tris egg yolk diluent on the quality and kinematic characteristics of frozen semen from Bali bulls.

Methods: Fresh and frozen semen samples were obtained from Bali bull and divided into four different treatment groups. P0 contained semen samples + diluent, while P1 to P3 consisted of semen samples + diluent supplemented with EGCG levels of 0.1, 0.15, and 0.2 mg/100 ml, respectively. Data were analyzed using One-way ANOVA and followed by Duncan's test if significant differences were found (p<0.05). Parameters observed included the assessment of fresh semen quality, kinematic analysis, post-thawing sperm viability, and abnormality.

Results: The results indicated that the assessment of fresh semen quality showed macroscopic and microscopic semen quality according to SNI 4869-1:2021. Kinematic analysis revealed significant differences in DSL and STR parameters between P0 and P3 (p<0.05). EGCG supplementation also caused significant differences in motility between P0 and P3 (p<0.05). Viability and spermatozoa abnormality with EGCG supplementation did not show significant differences (p>0.05).

Conclusion: The best results for motility, kinematics, and sperm morphology variables were found in P1 as it did not exhibit a decrease in motility, kinematics, and sperm morphology. Viability did not show significant differences between P1, P2, and P3, but the best results were found in P2 as it did not exhibit a decrease in viability with mean and standard deviation (66.84 ± 7.88). Abnormality variables also did not show significant differences between P1, P2, and P3, but the best results were found in P2 as it did not exhibit a decrease in abnormality with mean and standard deviation (23.80 ± 7.36).

背景:目的:本研究旨在探讨在三蛋黄稀释液中添加纯化绿茶提取物抗氧化剂表没食子儿茶素-3-棓酸盐(EGCG)对巴厘公牛冷冻精液的质量和运动特性的影响:从巴厘公牛身上获取新鲜和冷冻精液样本,并将其分为四个不同的处理组。P0 组为精液样品+稀释液,P1 至 P3 组为精液样品+稀释液,并分别添加 0.1、0.15 和 0.2 mg/100 ml 的 EGCG。数据采用单因素方差分析,如发现显著差异,则进行邓肯检验(pResults):结果表明,根据 SNI 4869-1:2021,新鲜精液质量评估显示了精液的宏观和微观质量。运动学分析表明,P0 和 P3 的 DSL 和 STR 参数存在显著差异(ppp>0.05):P1的精子活力、运动学和精子形态变量结果最好,因为它的活力、运动学和精子形态都没有下降。活力在 P1、P2 和 P3 之间没有明显差异,但 P2 的结果最好,因为其活力没有下降,平均值和标准偏差(66.84 ± 7.88)。畸变变量在 P1、P2 和 P3 之间也没有显示出显著差异,但 P2 的结果最好,因为它的畸变率在平均值和标准偏差(23.80 ± 7.36)上都没有下降。
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引用次数: 0
The immunostimulatory effects of fucoidan on the cellular and humoral immune response in Wistar rats. 褐藻糖胶对 Wistar 大鼠细胞和体液免疫反应的免疫刺激作用。
IF 0.9 Q3 VETERINARY SCIENCES Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI: 10.5455/OVJ.2024.v14.i8.7
Rochmah Kurnijasanti, Giftania Wardani, Muhammad Rais Mustafa, Sri Agus Sudjarwo

Background: Natural product active ingredients are currently being studied rigorously worldwide and offer a viable substitute for traditional immunotherapy for various medical disorders.

Aim: The objective of the study was to investigate the immunostimulatory properties of fucoidan in albino Wistar rats.

Methods: For the current study, forty rats were divided into five groups of rats that were used in good condition. In-vivo experiments of fucoidan were carried out in Wistar albino rats, such as the cyclophosphamide-caused myelosuppression, the delayed-type hypersensitivity (DTH) response, the phagocytic activity, the haemagglutinating antibody (HA) titer, and the neutrophil adhesion test.

Results: The phagocytic index increased significantly in response to Fucoidan in a dose-dependent manner, as well as enhanced DTH reaction, and HA titer caused by sheep red blood cells sheep red blood cells. Additionally, fucoidan decreased myelosuppression in rats after cyclophosphamide treatment and enhanced neutrophil adhesion with nylon fiber.

Conclusion: These findings imply that fucoidan has immunostimulant properties and could potentially utilised to treat immune-depression diseases.

背景:目的:本研究旨在探讨褐藻糖胶在白化 Wistar 大鼠体内的免疫刺激特性:在本次研究中,40 只大鼠被分为 5 组,每组大鼠的状况良好。在 Wistar 白化大鼠体内进行了褐藻糖胶的体内实验,如环磷酰胺引起的骨髓抑制、迟发型超敏反应(DTH)、吞噬细胞活性、血凝抗体(HA)滴度和中性粒细胞粘附试验:结果:褐藻糖胶能明显提高吞噬细胞指数,且呈剂量依赖性,还能增强绵羊红细胞引起的DTH反应和HA滴度。此外,褐藻糖胶还能减轻环磷酰胺治疗后大鼠的骨髓抑制,增强中性粒细胞与尼龙纤维的粘附性:这些研究结果表明,褐藻糖胶具有免疫刺激特性,可用于治疗免疫抑制疾病。
{"title":"The immunostimulatory effects of fucoidan on the cellular and humoral immune response in Wistar rats.","authors":"Rochmah Kurnijasanti, Giftania Wardani, Muhammad Rais Mustafa, Sri Agus Sudjarwo","doi":"10.5455/OVJ.2024.v14.i8.7","DOIUrl":"10.5455/OVJ.2024.v14.i8.7","url":null,"abstract":"<p><strong>Background: </strong>Natural product active ingredients are currently being studied rigorously worldwide and offer a viable substitute for traditional immunotherapy for various medical disorders.</p><p><strong>Aim: </strong>The objective of the study was to investigate the immunostimulatory properties of fucoidan in albino Wistar rats.</p><p><strong>Methods: </strong>For the current study, forty rats were divided into five groups of rats that were used in good condition. In-vivo experiments of fucoidan were carried out in Wistar albino rats, such as the cyclophosphamide-caused myelosuppression, the delayed-type hypersensitivity (DTH) response, the phagocytic activity, the haemagglutinating antibody (HA) titer, and the neutrophil adhesion test.</p><p><strong>Results: </strong>The phagocytic index increased significantly in response to Fucoidan in a dose-dependent manner, as well as enhanced DTH reaction, and HA titer caused by sheep red blood cells sheep red blood cells. Additionally, fucoidan decreased myelosuppression in rats after cyclophosphamide treatment and enhanced neutrophil adhesion with nylon fiber.</p><p><strong>Conclusion: </strong>These findings imply that fucoidan has immunostimulant properties and could potentially utilised to treat immune-depression diseases.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142292776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Open Veterinary Journal
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