Open Veterinary Journal最新文献

Background: Cervical cancer is a severe concern for women throughout the world. This percentage of cancer incidence causes sufferers to die at a high rate. It is believed that the bark of the Pinus merkusii tree contains anti-cancer compounds that inhibit cervical cancer cell growth.

Aim: This present study aims to examine the cytotoxic ability of P. merkusii tree bark ethanol extract (PMBE) by inducing apoptosis in HeLa cells.

Methods: We administered the PMBE at concentrations of 50, 100, 200, and 400 µg/ml to HeLa cell cultures. We then conducted the MTT cytotoxicity assay, detected apoptosis via Annexin V binding, and observed caspase 9 expression via immunocytochemistry.

Results: PMBE showed cytotoxic activity on HeLa cells with an IC₅₀ of 226.6 µg/ml for 24 hours of treatment. PMBE caused early apoptosis in up to 81.31% of HeLa cells, as well as increased caspase-9 expression.

Conclusion: Based on this study, PMBE is predicted to have dose-dependent antiproliferative or cytotoxicity effects on the HeLa cell line through the intrinsic pathway apoptosis mechanism.

Background: Antibiotic use has been rising in both humans and animals. The growing concern over antimicrobial drug resistance and the promotion of regional drug use have led to a rise in the interest in medicinal applications of herbs combined with biosynthesized nanoparticles.

Aim: To evaluate the antimicrobial and acaricidal effects of Syzygium cumini leaves crude extract (Sc-CE) and biosynthesized S. cumini silver nanoparticles (Sc-AgNPs) on dog skin pathogens and determined the optimal concentration and time for in vitro application.

Methods: Syzygium cumini leaves (Sc) were prepared as Sc-CE and Sc-AgNPs. The biosynthesized silver nanoparticles were characterized employing various techniques, including dynamic light scattering, scanning electron microscopy, and energy-dispersive X-ray analysis. Phytochemical analyses were conducted using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry screening. Antimicrobial activity was examined against gram-positive bacteria, including Staphylococcus aureus and Staphylococcus pseudintermedius, gram-negative bacteria such as Pseudomonas aeruginosa, yeast strains including Malassezia pachydermatis and Candida albicans, and ectoparasite. Cytotoxicity was evaluated on canine primary dermal fibroblast (CPDF) using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.

Results: The Sc-AgNPs exhibited nanoparticle sizes ranging from 100 to 350 nm with aggregated spherical shape and contained Ag element in this nanoparticle. Myricetin and Phloretin were among the extracted compounds, contributing to the reduction of pathogenic organisms. Sc-AgNPs showed high efficacy against skin pathogens compared to Sc-CE, with a lower cytotoxicity effect on CPDF.

Conclusion: The Sc-AgNPs demonstrated superior efficiency against pathogens in dog skin diseases as both concentration- and time-dependent and were deemed safe to CPDF within 24 hours.

Background: Humans and animals who have an acute case of diarrhea can be infected with Cryptosporidium spp. Within the category of water-borne disease, it is a zoonotic disease. The zoonotic disease Cryptosporidium is among the several pathogens carried by wild rats (Rattus spp.). The risk of spreading this disease is rather significant in urban environments because rats are often close to people.

Aim: This study aims to detect Cryptosporidium spp. infection in wild rats in Surabaya, East Java, Indonesia.

Methods: Through necropsy, a total of 100 wild rats' intestines were sampled for feces. Microscopic observation of the presence of Cryptosporidium was carried out using the float test with a combination of Ziehl Neelsen (ZN) staining. Molecular detection of Cryptosporidium spp. positive results used the Cryptosporidium oocyst wall protein (COWP) gene with polimerase chain reaction method.

Results: The results showed that 69 samples were positive for containing Cryptosporidium spp. oocysts and with ZN staining to confirm the diagnosis, the staining results showed Cryptosporidium spp. oocysts dark pink with a clear cavity inside with a percentage of 95.65% in Rattus norvegicus and 61.03% in Rattus tanezumi. In residential and densely populated environments the percentage of Cryptosporidium spp. amounted to 66.66% and in the market environment amounted to 74.19%. The percentage of Cryptosporidium spp. in the North Surabaya region was 42.85%, South Surabaya 100%, West Surabaya 37.5%, East Surabaya 81.39%, and Central Surabaya 65.38%. Molecular detection of Cryptosporidium spp. positive results were obtained using the COWP gene 550 bp.

Conclusion: This study aims to detect Cryptosporidium spp. infection in wild rats in Surabaya, East Java, Indonesia. The high number of cases of cryptosporidiosis in wild rats has the potential to be a reservoir for the spread of the disease. The Cryptosporidium spp can detected with COWP in 550 bp in wild rats in Surabaya, East Java, Indonesia.

Background: Cat bites to people are an important public health problem. However, very little information exists on the epidemiology of people bitten by cats at the level of an entire country in Latin America. Additionally, the COVID-19 pandemic significantly influenced people's lives and their relationships with pets, which could potentially affect the frequency of people bitten by cats.

Aim: The aim of this study was to determine the prevalence of people bitten by cats in Uruguay (2010 to 2020) according to the sex and age of the victim, season of the year, and to compare the prevalence in the pandemic year (2020) with that of the pre-pandemic years (2010-2019).

Methods: Cross-sectional study. Cat-bite notifications for the 2010-2020 period were analyzed using data from the Uruguayan Ministry of Public Health (Uruguay).

Results: The annual cat-bite rate for the 2010-2020 period was 2.1 per 100,000 people. The frequency of cat bites varied with the victim´s sex (p < 0.0001), being greater in females (n = 506, 66.5%) than in males (n = 255, 33.5%). The age at being bitten by cats varied with the sex of the victim, with males being bitten at an earlier age than females (p < 0.0001). Among the age categories of 30-74 years, females were bitten more than males (p < 0.05). Overall, cat bites were much less frequent in the oldest age categories (over 75 years old, p < 0.05). The percentage of cat bites tended to change with the season of the year (p = 0.08), with most bites occurring in spring and summer. The frequency of cat-bite injuries was similar between 2020 and the pre-COVID-19 pandemic years.

Conclusion: In Uruguay, cat bite incidents were affected by the victim's sex (more in females) and age, as well as by the season. During the initial year of the COVID-19 pandemic, the rate of cat bites was no different from those in earlier years.

Background: Diabetes mellitus is a significant global health issue with increasing prevalence worldwide.

Aim: This study aims to investigate the potential of Tithonia diversifolia extract (TE) in lowering interleukin-35 (IL-35), tumor necrosis factor-alpha (TNF-α), and hematological profile in streptozotocin (STZ)-induced rats.

Methods: A total of 24 rats were divided into four treatment groups: control (P0), diabetic induction (P1), diabetic induction + TE (P2), and diabetic induction + quercetin (P3). Diabetes mellitus was induced by a single-dose injection of stz (60 mg/kg). TE treatment was administered orally for 7 days. On the 8th day post-treatment, all animals were euthanized, and blood samples were collected to assess inflammatory parameters, including IL-35, TNF-α, GPx, and hematological profiles. Kidney organs were fixed in 10% buffered neutral formalin for histopathological analysis. Data were analyzed using ANOVA followed by Duncan's test (p < 0.05).

Results: Evaluation of the hematological profile revealed significant improvements in the P2 and P3 groups, with decreased leukocytes, hemoglobin, lymphocytes, and neutrophils, as well as significantly lower IL-35 and TNF-α levels observed in diabetic rats following TE treatment.

Conclusion: TE treatment exhibited promising effects in reducing inflammatory markers and restoring hematological parameters in diabetic rats, indicating its potential as a therapeutic agent in diabetic rats.

Background: Camel milk has emerged as a potential complementary therapy for autism spectrum disorder (ASD).

Aim: This study aimed to gather evidence from randomized controlled trials (RCTs) on the effectiveness of camel milk consumption in improving symptoms and associated measures in children with ASD.

Methods: Comprehensive searches of multiple databases were conducted up to March 14, 2024, for RCTs that evaluated whether camel milk consumption by children with ASD was more beneficial than the consumption of a control substance. Quality and bias analyses and meta-anlaysis data were synthesized and analyzed.

Results: Of 136 records identified, 5 RCTs (n = 299 children) were selected. The mean difference in scores on the childhood autism rating scale (CARS) for the group given camel milk and the control groups was a mean deviation (MD) ‒0.75, 95% CI‒1.97 to 0.47, p = 0.23. The mean difference in CARS scores in the subgroup analyses for raw camel milk was MD‒0.95, 95% CI‒2.33 to 0.44, p = 0.18 and boiled camel milk MD ‒0.50, 95% CI‒1.93 to 0.93, p = 0.49. A qualitative synthesis found that raw camel milk intake led to improvements in various social behaviors in children with ASD. Camel milk consumption resulted in increased levels of anti-inflammatory, antioxidant, and immunomodulatory biomarkers, with some differences observed between patients given raw camel milk and boiled camel milk.

Conclusion: Camel milk shows promise in improving social behaviors and certain biochemical markers in children with ASD, although the current meta-analysis did not document a significant statistical difference in CARS scores for the children studied. Future studies should focus on rigorous RCTs and larger sample sizes to substantiate these preliminary findings.

Background: Since its discovery in 1926, Newcastle disease (ND) is still emerging in many avian species worldwide causing severe economic losses due to high mortality.

Aim: This article aims to discuss the challenge of virulent ND in poultry in Libya, focusing on recent outbreaks investigated in Alzintan, Alrayaina, Nalut, and Surman, cities located in the western region of Libya.

Methods: Clinical signs and lesions were recorded. Tissues, as well as tracheal and cloacal swabs, were collected. RNA extraction was performed for confirmation using PCR and sequencing.

Results: Mortality, in general, reached 50%-100% in vaccinated flocks with respiratory distress, diarrhea, swelling of the face, and nasal discharges. Necropsy revealed severe hemorrhages in the proventriculus, necrosis, and hemorrhages in the intestine and cecal tonsils. All tested samples were positive for avian orthoavulavirus 1 (AOAV-1) using rRT-PCR and genetic analysis. The sequences obtained are referable to AOAV-1, which is the same strain in all tested samples. The amino acid sequences deduced from the cleavage site of the F protein are referable to a velogenic strain of AOAV-1 belonging to genotype VII.2. The detected strains in the current study revealed 86%-91% identity with European isolates identified between 2020 and 2022 and isolates from Asia and Africa and 97% identity to the previous isolated Libyan strains in 2013 and 2016. It is slightly different by the presence of amino acid lycine at position 111 on the cleavage site of the F0 gene as compared to previous Libyan strains in which arginine was found in the same position. The nucleotide sequence at this position changed from (aga) in AOAV-1 strains of years 2013 and 2016 to (aag) of the year 2023.

Conclusion: ND remains a significant threat to the poultry industry in Libya. Therefore, there is an urgent need to conduct an epidemiological study with a representative number of samples from all regions of the country, alongside the implementation of an inactivated vaccine targeting genotype VII.

Background: The Sunda porcupine, or the Javan porcupine (Hystrix javanica), is a rodent native to Indonesia. Although information about its conservation status is available, the hematological profile is limited. The normal hematological profile of an animal is essential as a basic health indicator to determine the initial state of a pathological process of a disease. Since the hematological profile can provide significant evidence regarding the health status of the species, the Sunda Porcupine's hematological profile can benefit conservation initiatives. Therefore, hematological evaluation of these porcupines can play a role in conservation initiatives, breeding strategies, and the prevention of zoonotic diseases.

Aim: This research revealed the routine blood evaluation and white blood cell (WBC) morphological features of the free-ranging Sunda porcupine (H. javanica).

Methods: Blood samples from four free-range individuals captured Sunda porcupine (H. javanica) were obtained intracardially for routine hematological evaluation and WBC staining for morphological identification. The profiles were then analyzed descriptively.

Results: Hematology profile averages were generally 4.04 × 10⁶/Ul for RBC; Hemoglobin was in 12.83 g/dl; Hematocrit by 37.8%; MCV 107.1 fl; MCH 31.80 pg; MCHC 31.23 g/dl; while WBC and platelet (PLT) were at 9.67 × 10³/μl and 503.00 × 10³/μl, respectively.

Conclusion: The morphology of red blood cells, WBCs, and the hematological profile of the Javan hedgehog is not much different from that of other mammals such as crested porcupines, ferrets, and guinea pigs. Until now, there has been limited research on the hematology of Sunda porcupines, which has led to a limited understanding of their health status. We anticipate that the findings of this study will serve as a diagnostic instrument for evaluating the health condition of Sunda porcupine and serve as a benchmark.

Background: Porcine epidemic diarrhea virus (PEDV) is a recurring coronavirus that causes severe diarrhea in pigs with high mortality and morbidity rates, especially in neonatal pigs. Despite the availability of vaccines, their efficacy is limited owing to antigenic differences between the vaccine and field strains, which poses a challenge to infection control. Antiviral drugs targeting conserved PEDV proteins show promise for complementing vaccination strategies. PEDV Nsp3 (PL2Pro) and Nsp5 (3CLPro) are essential proteases vital for viral replication, making them attractive targets for drug development against PEDV.

Aim: To address the lack of therapeutics against recurring PEDV outbreaks and bridge the gap in the application of bioinformatics in veterinary drug discovery, this study aimed to discover compounds that inhibit PEDV proteases from Philippine medicinal plants by applying a modified virtual screening methodology that considers the physiology of swine hosts.

Methods: This study employed a library of 690 metabolites from Philippine medicinal plants to screen for potential protease inhibitors targeting PEDV PL2Pro and 3CLPro. This includes evaluating the binding affinity, pharmacokinetics, dynamic stability, and critical binding site residues. Compounds demonstrating high affinity underwent a modified ADMET analysis, considering the enteric localization of the virus and potential toxicity to swine hosts. Furthermore, molecular dynamics simulations assessed compound stability under physiological swine conditions.

Results: The study identified Bisandrographolide from Andrographis paniculata, CID 162866964 from Euphorbia neriifolia, and betulinic acid from Vitex negundo and Ocimum basilicum as metabolites that bind favorably and selectively to PEDV 3CLPro and have excellent pharmacokinetic properties and dynamic stability. In contrast, no selective inhibitor for PL2pro passed the same criteria.

Conclusion: Employing the modified virtual screening protocol tailored for swine host considerations, the compounds identified in this study are anticipated to exert inhibitory effects against PEDV without off-target binding to analogous swine proteases and receptors. CID 162866964, bisandrographolide, and betulinic acid show promise for developing potent antivirals against PEDV.

Background: Klebsiella pneumoniae is an important opportunistic pathogen, which is capable of colonizing the respiratory system in both humans and animals causing mild to severe infections.

Aim: This study aims to isolate K. pneumoniae from the nasal discharges of human and sheep as well as identify the antibiotic resistance and molecular phylogeny of local isolates.

Methods: A total of 100; 50 humans and 50 sheep, positive nasal swab isolates were selected, and confirmed biochemically and by the VITEK-2 system. Molecular testing using the polymerase chain reaction (PCR) and phylogeny was conducted.

Results: On MacConkey agar, Klebsiella isolates appeared as large, pinkish, and mucoid colonies; while microscopically, it appeared as Gram-negative rods. Traditional biochemical tests revealed that 62% and 78% of human and sheep isolates were positive Klebsiella isolates, whereas respectively, 54.84% and 71.8% of these isolates were positive by VITEK-2. Antibiotic susceptibility tests showed that the human isolates were sensitive to aztreonam, piperacillin-tazobactam, ciprofloxacin, and cefuroxime. Subsequently, sheep isolates were sensitive to cefuroxime, ciprofloxacin, piperacillin-tazobactam, ampicillin, cefoxitin, and tetracycline. Targeting 16S rRNA gene, a total of 17 human and 28 sheep isolates were molecularly positive K. pneumoniae. Phylogenetic analysis of study human and sheep isolates showed their identity to NCBI Indian (LC747146.1) and Iraqi (LC711141.1) isolates, respectively. Comparative analysis between the local human and sheep isolates revealed a significant identity that ranged from 99.82% to 99.88% with a percentage of mutation ranging from 0.008% to 0.002%.

Conclusion: Klebsiella pneumoniae is a highly prevalent bacterium in both human and sheep with an observable resistance to antibiotics. Molecular phylogeny of study isolates demonstrated their close relation, suggesting the possible direct or indirect transmission of the bacterium from sheep to human or vice versa. Moreover, studies are greatly important to estimate the routes of bacterial transmission. Also, extensive hygiene practices could be lowered the spreading of K. pnuemoniae to farm workers.