One Health最新文献

{"title":"High throughput sequencing in the diagnosis and surveillance of tick-borne diseases: A narrative review","authors":"Yannick-Ibrahim Bisso ,&nbsp;Olivier Duron ,&nbsp;Olivier Plantard ,&nbsp;Gilles Prevost ,&nbsp;Benoit Jaulhac ,&nbsp;Pierre Boyer","doi":"10.1016/j.onehlt.2025.101312","DOIUrl":"10.1016/j.onehlt.2025.101312","url":null,"abstract":"<div><div>Ticks are blood-feeding arthropods that can transmit a wide variety of microorganisms (bacteria, viruses, protozoa, and filarial nematodes) when they feed on various vertebrate hosts. In the recent years, high-throughput sequencing (HTS), also called next-generation sequencing, has become a key tool for detecting and characterizing microorganisms, whether they are pathogens or part of the tick's own microbiota. This narrative review summarizes current applications of HTS for the surveillance and diagnosis of tick-borne diseases within a One Health framework.</div><div>From nucleic acids extracted from a tick sample, HTS enables the possibility of simultaneous detection of multiple microorganisms, and provides valuable information on potential reservoir hosts through blood meal analysis. It has revealed a vast diversity of bacterial, viral, protozoan agents and filarial nematodes in various tick species worldwide, including unexpected or novel pathogens. HTS has also improved our understanding of the tick microbiota and how it interacts with pathogens, which could have an impact on vector competence.</div><div>In the field of microbiological diagnosis, HTS provides a complementary or alternative approach to traditional diagnostic tests, particularly in cases with non-specific symptoms or when the etiology is unknown. HTS has proven to be effective in detecting rare or novel pathogens, including some transmitted by ticks. It has also enabled the reconstruction of whole genomes of microorganisms from clinical samples or ticks, thereby improving our understanding of the molecular epidemiology of these agents.</div><div>By bridging the vector, its pathogens, the reservoir host, and human or animal clinical outcomes, HTS represents a cornerstone technology for future integrated surveillance systems of TBDs within a One Health perspective.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101312"},"PeriodicalIF":4.5,"publicationDate":"2025-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using participatory epidemiology to understand differences in tick-borne disease perceptions from homeowners in the Ohio River valley","authors":"Emily G. Bache ,&nbsp;Paulina Matzkin ,&nbsp;Jessica Fleming ,&nbsp;Risa Pesapane ,&nbsp;Danielle M. Tufts","doi":"10.1016/j.onehlt.2025.101309","DOIUrl":"10.1016/j.onehlt.2025.101309","url":null,"abstract":"<div><div>Tick-borne diseases (TBDs) are the most common vector-borne diseases reported annually in the United States. Lyme disease, caused primarily by the spirochetal bacterium <em>Borrelia burgdorferi</em> sensu stricto (s.s.) affects an estimated 476,000 people each year. <em>Borrelia burgdorferi</em> is transmitted by the tick <em>Ixodes scapularis</em> and is maintained in an enzootic cycle with small mammal reservoirs in the eastern United States. Since <em>B. burgdorferi</em> can infect humans and domestic animals, Lyme disease is an important One Health concern between humans and animals. People residing in endemic areas are likely to possess differences in knowledge and protective behaviors compared to people living in areas where Lyme disease is newly emerging. To better understand behavioral differences and perspectives of Lyme disease and other TBDs in these areas, elements of participatory epidemiology were incorporated by surveying homeowners from a Lyme disease endemic area (southwestern Pennsylvania) and an emerging area (eastern Ohio). The survey covered three main areas of interest: perceived risk of tick exposure and TBD health risks, personal protection methods used to prevent or reduce exposure to tick bites, and protection methods to reduce tick presence on residential properties. The results of our study suggest that residents in endemic regions may be more educated and aware of TBD risk and prevention methods based on their belief that TBDs pose at least a minor health risk. Additionally, more Pennsylvania residents used tick-bite prevention methods while outdoors on their property. Residents in Ohio were unlikely to make any changes to protect themselves or their property from ticks, which may be a result of a lack of education or motivation to change their behaviors. The findings from this study can inform public health education and interventions to increase awareness and reduce the risk of TBDs in endemic and emerging areas.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101309"},"PeriodicalIF":4.5,"publicationDate":"2025-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The niche of Borrelia burgdorferi sensu lato in Europe is predictable and mappable","authors":"Agustín Estrada-Peña ,&nbsp;Julie Davis ,&nbsp;James H. Stark ,&nbsp;Patrick H. Kelly","doi":"10.1016/j.onehlt.2025.101313","DOIUrl":"10.1016/j.onehlt.2025.101313","url":null,"abstract":"<div><div>Classic environmental niche modelling to examine the distribution of the causative agent of Lyme borreliosis <em>Borrelia burgdorferi</em> sensu lato (<em>Bb</em>) in the Western Palearctic, is often inadequate because it depends on both its tick vector(s), like <em>Ixodes ricinus</em>, and vertebrate reservoirs. We aimed to better determine, identify, and map the geographic distribution of <em>Bb</em> genospecies compiling data from 15,032 <em>I. ricinus</em> samples and over 6.5 million vertebrate records across 103 genera. We leveraged Species Stacking Distribution Modelling and Principal Components Analysis to identify communities of co-occurring vertebrates and their associations between <em>Bb</em> prevalence in host-seeking <em>Ixodes</em> nymphs and. Four vertebrate communities were revealed, with one strongly linked as primary reservoirs to <em>Bb</em> geographic range. Distribution of <em>Bb</em> in southern Europe was limited by the absence of <em>I. ricinus</em> despite suitable reservoirs, while in northern regions, a lack of competent reservoirs restricts its spread. The <em>Bb</em> prevalence in questing ticks correlates significantly (R² = 0.89) with the presence of key reservoirs rather than overall vertebrate diversity which suggests the <em>Bb</em> niche is predictable and tied to specific vertebrate-tick co-occurrences. We compiled a dataset with the climate, vegetation, and vertebrate-derived variables linked to the transmission pressure of <em>Bb</em> to humans for the complete European territory, aiming for the prevention of infection in humans. This research underscores the importance of integrating reservoir species and tick distribution data to better map and predict <em>Bb</em> spread. By capturing the effects of climate and community composition on the occurrence of <em>Bb</em> in Europe, this framework provides insights for tracking Lyme borreliosis at a continental-level.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101313"},"PeriodicalIF":4.5,"publicationDate":"2025-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic epidemiology and phylogeographic reconstruction of West Nile virus 2 in Italy from 2011 to 2023","authors":"Carla della Ventura ,&nbsp;Maya Carrera ,&nbsp;Francesco Defilippo ,&nbsp;Davide Lelli ,&nbsp;Chiara Nogarol ,&nbsp;Maria Lucia Mandola ,&nbsp;Alessia Lai ,&nbsp;Annalisa Bergna ,&nbsp;Francesca Moroni ,&nbsp;Ana Moreno ,&nbsp;Gianguglielmo Zehender","doi":"10.1016/j.onehlt.2025.101310","DOIUrl":"10.1016/j.onehlt.2025.101310","url":null,"abstract":"<div><div>Since its introduction to Europe in 2004, West Nile Virus Lineage 2 (WNV-2) has become endemic, with Italy reporting the highest number of cases each season. In 2022, WNV infections in Italy exceeded those recorded during the major 2018 outbreak—the largest ever reported in Europe. This study investigates the genomic epidemiology of WNV during the 2022 and 2023 transmission seasons.</div><div>We analyzed 123 environmental samples from wild birds and mosquito pools collected between May and October 2022–2023 in northwestern Italy. All but one sample belonged to Lineage 2; lineage 1 was detected in two bird samples, with one showing co-infection. A total of 98 complete genomes were sequenced.</div><div>Phylogeographic reconstruction indicated the origin of the main European clade in Hungary in 2004, with introduction into Italy between 2009 and 2010. Most Italian genomes clustered within a single highly supported subclade, with one sampled in the Marche region in 2011 as the outgroup. Continuous phylogeographic analysis suggested the Italian WNV-2 clade originated in 2009 in the area between Emilia-Romagna and Lombardy, followed by east-west spread during 2022–2023.</div><div>Several mutations were identified, including F49L in the NS2A gene linked to neuronal tropism, and M184V in the NS4B gene, associated with increased pathogenicity.</div><div>Our results highlight how integrated genomic surveillance of WNV, combining whole genome sequencing and phylogenetic analyses to environmental samples, can support One Health approaches for early detection and risk assessment of arboviral transmission.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101310"},"PeriodicalIF":4.5,"publicationDate":"2025-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knowledge, attitudes, and practices of women related to human and animal feces management in Ethiopia: Evidence for strengthening water, sanitation, and hygiene (WaSH) initiatives","authors":"Birhanu Hadush ,&nbsp;Chaltu Fikru ,&nbsp;Girma Kebede ,&nbsp;Syum Guesh ,&nbsp;Enquebaher Kassaye ,&nbsp;Getachew Redae ,&nbsp;Kebede Amenu ,&nbsp;Lisa Cavalerie ,&nbsp;Siobhan M. Mor","doi":"10.1016/j.onehlt.2025.101311","DOIUrl":"10.1016/j.onehlt.2025.101311","url":null,"abstract":"<div><h3>Background</h3><div>Water, sanitation, and hygiene (WaSH) programs aim to prevent feco-oral disease transmission, but their effectiveness in low- and middle-income countries is often compromised by frequent exposure to animal feces. In Ethiopia, national initiatives such as the Health Extension Program and Community-Led Total Sanitation and Hygiene program have expanded WaSH coverage but do not currently address animal-associated risks. This study investigated knowledge, attitudes, and practices related to human and animal feces management in Butajira, Gilgel Gibe, and Kilite Awlaelo Health and Demographic Surveillance Sites (HDSS) in Ethiopia.</div></div><div><h3>Methods</h3><div>A community-based, cross-sectional study was conducted through random sampling of households in selected kebeles across the three HDSS sites. Eligible households owned up to 60 livestock, including at least one head of cattle. Adult women (<em>n</em> = 1129) were interviewed using a structured questionnaire on WaSH and livestock husbandry, including manure management. Descriptive statistics were used to summarise practices across sites.</div></div><div><h3>Results</h3><div>The median age of participants ranged from 35 to 45 years, with female-headed households most frequent in Butajira (42.2 %). Literacy levels were lowest in Gilgel Gibe (64.1 % illiteracy). Most households accessed improved water sources, though unimproved sources were important in Gilgel Gibe (16.3 %). Improved toilet facilities were most common in Gilgel Gibe (61 %). At least 79 % of respondents reported handwashing after toilet use, with moderate soap use (&gt;73 %). Manure management was primarily the responsibility of adult women (&gt;74 %), often using bare hands. Handwashing with soap after manure handling was common in Kilite Awlaelo (&gt;97 %) but lower in Butajira (86.3 %) and Gilgel Gibe (74.8 %). Awareness of health risks from animal manure was lower than for human feces, especially in Gilgel Gibe (70 %) and Butajira (53.2 %). Similarly, awareness of the benefits of composting or drying manure to prevent germ spread was lower in these sites.</div></div><div><h3>Conclusions</h3><div>Despite high awareness of WaSH-related risks, critical gaps exist in understanding manure-associated health risks, particularly in Butajira and Gilgel Gibe. Targeted interventions are needed to raise awareness and improve manure management, especially among women.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101311"},"PeriodicalIF":4.5,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity and occurrence of antibiotic resistance genes from community wastewater in Dhaka and Cox's Bazar, Bangladesh","authors":"Akash Saha ,&nbsp;Rashedul Hasan ,&nbsp;Jenifar Quaiyum Ami ,&nbsp;Md Mahmudul Hasan ,&nbsp;Nuhu Amin ,&nbsp;Rehnuma Haque ,&nbsp;Mahbubur Rahman ,&nbsp;Farjana Jahan ,&nbsp;Rezaul Hasan ,&nbsp;Pengbo Liu ,&nbsp;Christine L. Moe ,&nbsp;Mohammad Enayet Hossain ,&nbsp;Mohammed Ziaur Rahman","doi":"10.1016/j.onehlt.2025.101301","DOIUrl":"10.1016/j.onehlt.2025.101301","url":null,"abstract":"<div><h3>Objectives</h3><div>Antibiotic resistance genes (ARGs), one of the key drivers of the growing antibiotic resistance, present a formidable public health concern. Wastewater may act as a key repository for both antibiotic-resistant bacteria and ARGs, facilitating their transfer across microbial communities. Given the limited knowledge of clinically relevant ARGs in community wastewater of Bangladesh, this study explored the genetic diversity and occurrence of ARGs from community wastewater discharges using a culture-independent approach.</div></div><div><h3>Methods</h3><div>Wastewater samples (<em>n</em> = 70) were collected from different sites in Dhaka and Cox's Bazar between April and May 2023. These samples underwent Nanotrap®-based culture-independent microbial concentration, followed by nucleic acid extraction and purification. Nine clinically important ARGs (<em>blaTEM</em>, <em>blaCTX-M</em>, <em>blaSHV</em>, <em>blaKPC</em>, <em>blaNDM</em>, <em>blaOXA</em>, <em>mcr-1</em>, <em>tolC</em>, and <em>acrA</em>) were detected using uniplex polymerase chain reaction (PCR), followed by Sanger sequencing. Bioinformatic analyses further explored the genetic diversity of the ARGs, as well as mutational analysis and molecular docking of <em>Klebsiella pneumoniae</em> carbapenemase (KPC).</div></div><div><h3>Results</h3><div><em>blaTEM</em>, <em>blaCTX-M</em>, <em>blaOXA</em>, <em>tolC</em>, and <em>acrA</em> were the most frequently detected ARGs (90 % each), followed by <em>blaNDM</em> (85.7 %), <em>blaKPC</em> (84.3 %), <em>blaSHV</em> (77.1 %), and <em>mcr-1</em> (52.9 %). The frequency of all ARGs was higher in Cox's Bazar compared to Dhaka, except for <em>mcr-1</em>. Co-occurrences of all nine ARGs were observed in 47.1 % of samples. Mutational analysis of KPC revealed unique missense mutations, and further docking demonstrated altered binding affinities and interactions between KPC-2 mutants and β-lactam antibiotics.</div></div><div><h3>Conclusion</h3><div>Our study provides the baseline data of clinically significant ARGs in local community wastewater in Bangladesh, reflecting the need for further surveillance to identify hotspots and guide antibiotic stewardship and wastewater management policies.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101301"},"PeriodicalIF":4.5,"publicationDate":"2025-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Invasive alien mammals pose zoonotic risks to human health in Europe","authors":"Paola Monguilod ,&nbsp;Belinda Gallardo","doi":"10.1016/j.onehlt.2025.101307","DOIUrl":"10.1016/j.onehlt.2025.101307","url":null,"abstract":"<div><div>The rise in zoonotic diseases is accelerating, with climate change expected to further intensify this trend. Invasive Alien Species (IAS) play an important role in the emergence and spread of zoonotic diseases by introducing both existing and novel pathogens to the regions they invade. Despite this, research on the role of IAS in spreading zoonotic diseases remains limited. Our study investigated the zoonotic risks posed by eight invasive mammal species prioritized for management in Europe. On average, each species was found to transmit 16 pathogens capable of causing severe diseases in humans, including Echinococcosis, Leptospirosis, Lyme neuroborreliosis, and Encephalitis.</div><div>We identified central and western Europe as significant disease hotspots. Climate change is facilitating the expansion of IAS into new areas, as warmer temperatures make previously inhospitable regions suitable. Future projections indicate a northeastward shift in their suitability by 2050. These changes vary by species, with the Siberian chipmunk losing up to 45 % of its suitability, while the gray squirrel could see a 26 % increase under a high-emissions scenario.</div><div>Finally, we found that 71 % of the human population lives in areas highly suitable for IAS establishment. Our findings underscore the health risks associated with IAS and highlight the need for further research into their role in disease dynamics. Addressing this issue is essential for developing effective public health strategies and mitigating future zoonotic disease outbreaks.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101307"},"PeriodicalIF":4.5,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracing the roots of One Health principles in nursing practice","authors":"Júlio Belo Fernandes ,&nbsp;Sónia Fernandes ,&nbsp;Cidália Castro ,&nbsp;Diana Vareta","doi":"10.1016/j.onehlt.2025.101306","DOIUrl":"10.1016/j.onehlt.2025.101306","url":null,"abstract":"<div><div>This article examines the historical roots of the One Health concept and highlights its deep convergence with Florence Nightingale's Environmental Theory. Although One Health gained prominence in the early 2000s, its intellectual foundations are much older, encompassing Indigenous knowledge systems, nineteenth-century public health, and the work of Virchow, Osler, and Schwabe. Through a historical–conceptual analysis of Nightingale's writings and key One Health milestones, the article shows how her emphasis on environmental determinants, stewardship of natural and built environments, systematic observation, statistical evidence, and coordinated cross-sectoral action anticipated core One Health principles. Five areas of convergence are identified: prevention focused on environmental determinants, environmental stewardship, data-informed decision making, transdisciplinary collaboration, and continuous system improvement. The article argues that Nightingale's legacy positions nursing as a strategic actor in the local implementation of One Health, particularly in designing therapeutic and healthy environments and integrating environmental indicators into care, governance, and health policy.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101306"},"PeriodicalIF":4.5,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A claim for plant health as a key component of the one health concept","authors":"Ramon Albajes ,&nbsp;María M. López ,&nbsp;Rafael M. Jiménez Díaz","doi":"10.1016/j.onehlt.2025.101304","DOIUrl":"10.1016/j.onehlt.2025.101304","url":null,"abstract":"<div><div>The concept of health has historically been more closely linked to the well-being of humans than to that of animals, plants or the environment. In contrast, the One Health concept, which emerged in recent decades, generally recognizes the interdependence of only three of its four components: humans, wild and domestic animals, and the environment, but plants have often been overlooked in this analysis. Because plant health has been undervalued within the One Health framework, we emphasize in this review its importance in ensuring food security and safety, two key issues in human and animal health, as highlighted in several of the United Nations SDG. Food production, marketing and consumption use a significant portion of the environment's natural resources, and plant health technology must ensure their sustainable use while safeguarding environmental health. We summarize the relationships between the four components of One Health, highlighting the development of antimicrobial resistance in human, animal and plant pathogens, and the resistance to plant protection products among plant pests, diseases and weeds. Three external drivers significantly influence plant health and One Health in the last decades: climate change, invasive alien species, and the international armed conflicts. The main reported effects of climate change on plant health include the shifts in distribution area, biology and life cycles of harmful organisms as well as plant-pest interactions. Another major factor compromising the sustainability of current plant health technology is the introduction and establishment of alien organisms affecting vegetables resulting from the increasing globalization of food trade, human labour and tourism.These challenges underscore the need to approach One Health at broader scales, beyond individual organisms or populations, as well as intensifying studies on plant health, to effectively address holistically the complex, interconnected risks affecting global health.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101304"},"PeriodicalIF":4.5,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomic surveillance of tick-borne pathogens and microbiomes in Huntingdon County, Pennsylvania","authors":"Andrew Buonaccorsi ,&nbsp;Brittney N. McMullen ,&nbsp;Brie Builder ,&nbsp;Kelliann Drummond ,&nbsp;Sarah Halteman ,&nbsp;Jeremy Chen See ,&nbsp;Evan Thomas ,&nbsp;Alexa Viands ,&nbsp;Sarah Worley ,&nbsp;Justin R. Wright ,&nbsp;Jill Keeney ,&nbsp;Regina Lamendella","doi":"10.1016/j.onehlt.2025.101305","DOIUrl":"10.1016/j.onehlt.2025.101305","url":null,"abstract":"<div><div>The rise in tick populations across the United States has contributed to a surge in tick-borne diseases, with Pennsylvania ranking among the highest in reported cases. To better understand local pathogen prevalence and microbial community structure, an integrative study of ticks collected from ten recreational trails in Huntingdon County, Pennsylvania during the summer of 2023 was conducted. A total of 96 ticks were sampled, with 33 PCR-positive specimens selected for shotgun metagenomic sequencing. Pathogen screening via qPCR detected <em>Borreliella burgdorferi</em>, <em>Borrelia miyamotoi</em>, <em>Babesia</em> spp., and <em>Anaplasma phagocytophilum</em>. Shotgun metagenomics revealed a broader diversity of tick-borne pathogens, including <em>Rickettsia</em> and <em>Ehrlichia</em> spp., and demonstrated increased sensitivity by detecting low-abundance pathogens in samples that were PCR-negative. Co-infections were common, and multivariate statistical analysis identified significant associations between environmental variables (e.g., humidity, time of day, land cover) and microbial diversity and predicted gene function. Notably, diversity was higher in ticks collected during early afternoon and from northern sites. Co-occurrence network analysis showed <em>Rickettsia</em> as a central taxon with multiple significant positive associations with other microbes while other pathogens were largely absent or peripheral. These findings underscore the enhanced resolution of metagenomic approaches for pathogen detection and the value of combining molecular surveillance with ecological metadata. Our study provides critical insights into local tick microbiomes and pathogen prevalence, which may inform public health interventions and vector management strategies in central Pennsylvania.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101305"},"PeriodicalIF":4.5,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}