One Health最新文献

{"title":"Construction and validation of an evaluation index system for healthy villages in China: A human-AI synergistic approach integrating modified Delphi and AHP","authors":"Kai Wang,&nbsp;Yuanhao Hong,&nbsp;Yimei Pan,&nbsp;Liang Chen","doi":"10.1016/j.onehlt.2026.101352","DOIUrl":"10.1016/j.onehlt.2026.101352","url":null,"abstract":"<div><h3>Objective</h3><div>Research on healthy villages in China is currently constrained by limited evaluation criteria and a lack of systemic comprehensiveness. This study aims to develop a scientifically rigorous evaluation index system that is tailored to the regional characteristics of China.</div></div><div><h3>Method</h3><div>A modified Delphi method was employed to screen indicators based on literature review and expert consultation, followed by the Analytic Hierarchy Process (AHP) to determine the weights of these indicators. Innovatively, this study adopted a Human-AI synergistic approach throughout the research lifecycle; generative AI was utilized to refine indicator semantics during the Delphi phase, while an LLM-assisted comparative analysis served as a robustness check for the weighting system. Additionally, empirical validation was conducted in three pilot villages.</div></div><div><h3>Results</h3><div>The final system consists of 7 first-level, 31 s-level, and 61 third-level indicators. Metrics from expert consultations were satisfactory, with authority coefficients exceeding 0.80 and demonstrating strong coordination (<em>P</em> &lt; 0.001). Weight analysis indicated that “Healthy Population” (0.340) and “Healthy Life” (0.228) are the most critical dimensions. The robustness check revealed a strong correlation (Pearson's <em>r</em> = 0.99) between human expert consensus and AI-simulated weights, thereby confirming the system's validity. Furthermore, empirical application in three pilot villages produced distinct scores (97.1, 77.3, and 39.7), which accurately reflected developmental disparities and identified specific weaknesses for targeted intervention.</div></div><div><h3>Conclusion</h3><div>The constructed index system integrates multi-dimensional health factors with a scientifically rigorous design validated through this Human-AI synergistic strategy. Ultimately, this approach pioneers new pathways for the deep integration of artificial intelligence and public health management, while providing a reference model for developing comprehensive evaluation systems in other developing countries.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101352"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146188438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An overview of HPAI H5N1 clade 2.3.4.4b and its emerging threat in mainland Australia: Identified knowledge gaps","authors":"Pan Zhang,&nbsp;C Raina MacIntyre","doi":"10.1016/j.onehlt.2025.101292","DOIUrl":"10.1016/j.onehlt.2025.101292","url":null,"abstract":"<div><div>The highly pathogenic avian influenza (HPAI) H5N1 virus, particularly clade 2.3.4.4b, is currently causing a panzootic. The threat of its incursion into mainland Australia is escalating, especially following its detection in Antarctica and recently on Australia's sub-Antarctic territory, Heard Island. Comprehensive research on this emerging risk remains limited, partly due to ongoing rapid genomic mutations and evolving epidemiological dynamics. This review provides an in-depth analysis of clade 2.3.4.4b H5N1 and highlights critical knowledge gaps that must be addressed to mitigate the potential threat to mainland Australia.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101292"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145788998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling the impact of anthrax vaccination on buffalo outbreak dynamics in northern Vietnam","authors":"Francisca Javiera Rudolph ,&nbsp;Tan Minh Luong ,&nbsp;Thai My Do ,&nbsp;Van Binh Trinh ,&nbsp;Ba Uyen Pham ,&nbsp;Minh Dat Hoang ,&nbsp;Anh Hung Pham ,&nbsp;Van Truong Lu ,&nbsp;Van Khang Pham ,&nbsp;Thanh Long Pham ,&nbsp;Quang Thai Pham ,&nbsp;Thi Thu Ha Hoang ,&nbsp;Thi Mai Hung Tran ,&nbsp;Juan Pablo Gomez ,&nbsp;José Miguel Ponciano ,&nbsp;Jason K. Blackburn","doi":"10.1016/j.onehlt.2025.101294","DOIUrl":"10.1016/j.onehlt.2025.101294","url":null,"abstract":"<div><div>A widespread and underreported zoonosis, anthrax is a severe infectious disease of significant public health concern for humans, livestock, and wildlife. In this study, we used historical data from 1991 to 2020 from northern Vietnam and a simulation model to investigate the effects of different vaccination strategies on livestock outbreaks. We developed a novel approach combining semi-synthetic data generation and a sliding windows model fitting routine to estimate disease transmission parameters from surveillance data and address the temporal mismatch between pathogen transmission dynamics and disease reporting. Results showed that vaccination leads to a significant reduction in buffalo mortality, with reactive and increasing vaccination campaigns having the largest impact in reducing outbreak size. Reactive and decreasing vaccination campaigns initially controlled outbreaks, but mortality increased as soon as vaccination ceased, highlighting the need for sustained, long-term vaccination. In scenarios where populations had high natural immunity, the impact of vaccination was less pronounced, though still evident, suggesting that prioritizing vaccination efforts for more susceptible populations may provide a greater return on investment in outbreak prevention and control. Simulation models can offer valuable insights into vaccination and control strategies, providing tools to compare and evaluate potential outbreak scenarios. Our findings underscore the value of mathematical and simulation approaches to overcome data challenges and underreporting in global disease management for anthrax and other neglected diseases. We highlight the importance of continued investment in surveillance and modeling efforts, while providing a practical approach to optimize the use of existing data in Vietnam and similar settings.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101294"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145788934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virome analysis reveals ORF7 sequences of type 2 porcine respiratory and reproductive syndrome virus (PRRSV) for the first time in a rodent host (Microtus pennsylvanicus)","authors":"Elena Sgarabotto ,&nbsp;Nicola Zadra ,&nbsp;Jacob A. Tyrell ,&nbsp;Chiara Rossi ,&nbsp;Ian Hewson ,&nbsp;Jeremy B. Searle ,&nbsp;Heidi C. Hauffe","doi":"10.1016/j.onehlt.2026.101345","DOIUrl":"10.1016/j.onehlt.2026.101345","url":null,"abstract":"<div><div>The lung virome of meadow vole (<em>Microtus pennsylvanicus)</em> from six neighbouring locations in New York State, USA was investigated using metagenomics to determine the circulation of potentially zoonotic viruses in a common wild rodent. This study provides the first evidence of the occurrence of Type 2 Porcine Respiratory and Reproductive Syndrome Virus (PRRSV2) in a rodent, or indeed in any species apart from wild and domestic pigs (<em>Sus scrofa</em>). PRRSV has the highest economic impact on the pig husbandry industry of any pathogen, but up to now, farm-to-farm transmission of this virus has been assumed to be associated with movement of and contact with infected pigs and fomites. Our results showing the natural occurrence of this virus in potential transmission hosts other than wild or domestic pigs challenge this scenario. Phylogenetic analysis of assembled partial genomes from four of our pooled samples and all other nucleocapsid protein (ORF7) sequences available in Genbank showed that the sequences recovered from meadow voles unambiguously clustered within the PRRSV2 clade together with sequences derived from wild and domestic pigs<em>.</em> Historical research suggests that spillover from voles to domestic pigs may be the most parsimonious explanation for these results; however, we cannot rule out the reverse: that the source of PRRSV2 in these wild voles derives from pigs. From a One Health perspective, our results reinforce the importance of characterizing wildlife viromes to survey possible sources of zoonotic pathogens, which is vital for making evidence-based decisions regarding potential threats to the health of humans, livestock and wild fauna.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101345"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146181800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wet markets, live animals, and infection concerns in Thailand: A cross-sectional study","authors":"Robin Goodwin ,&nbsp;Katy Stokes ,&nbsp;Rewadee Watakakosol ,&nbsp;Panrapee Suttiwan ,&nbsp;Arunya Tuicomepee ,&nbsp;Juthatip Wiwattanapantuwong","doi":"10.1016/j.onehlt.2026.101346","DOIUrl":"10.1016/j.onehlt.2026.101346","url":null,"abstract":"<div><div>This study aimed to examine patterns of wet market attendance, live animal purchasing, and social contact behaviours in Thailand, and to assess perceived infection risks in these settings. A total of 1050 Thai residents across multiple regions of Thailand, aged 16–69 years (50.8% female) were recruited through street-intercept sampling. Inclusion criteria were age ≥ 16 and residence in Thailand; exclusion criteria included inability to complete the interview. Our results found, 42% of respondents reported attending wet markets at least several times per month. Attendance was higher amongst women, those with lower education, and smaller households. Frequent market attendees reported more physical contact (<em>r</em> = 0.20, <em>p</em> &lt; .001), perceived markets as more dangerous (<em>r</em> = −0.34, p &lt; .001), but also reported lower perceived control over infection. Seventy-nine respondents reported live animal purchases, with freshness and taste cited as primary motivations; participants who did so also expressed more concern about infection risk from the market, and perceived less control over potential infection. Protective behaviours such as handwashing (85.5%) and avoiding animal contact (71.3%) were widely reported. Wet markets remain a common setting for food purchasing in Thailand and are associated with increased social contact and perceived infection risk. These findings highlight the need for targeted One Health interventions that consider behavioural, demographic, and cultural factors. Further research should explore longitudinal changes in market behaviour and risk perception following novel zoonotic outbreaks such as COVID-19, particularly amongst high-exposure groups.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101346"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146166145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic insights into hepatitis E virus through environmental surveillance in Europe","authors":"Hao Wang ,&nbsp;Marianela Patzi-Churqui ,&nbsp;Linn Dahlsten Andius ,&nbsp;Kristina Nyström ,&nbsp;Martin Lagging","doi":"10.1016/j.onehlt.2025.101302","DOIUrl":"10.1016/j.onehlt.2025.101302","url":null,"abstract":"<div><div>Zoonotic hepatitis E has been a growing public health concern in Europe, but the transmission of its causative agent, hepatitis E virus (HEV), remains incompletely understood. Environmental surveillance, particularly through wastewater monitoring, has proven valuable for tracking viral circulation and variant shift during the COVID-19 pandemic, yet its application to HEV is still limited. In this review, we systematically analyzed HEV sequences across Europe, focusing on environmental sources from a genetic perspective. Of more than 13,100 HEV sequences deposited in the NCBI database, only 2.4 % (316/13,118) originated from environmental samples, including wastewater, surface water, and biosolids. Additional typing data from the literature revealed highly uneven geographic distribution, with 97 % of environmental sequences reported from Italy, France, the United Kingdom (UK), Spain, Sweden, and Germany. HEV-3 was the dominant genotype, while HEV-1 and HEV-4 were occasionally detected. Subtypes 3c and 3f were most common, but their prevalence varied across countries and sample types. Some countries, such as France, Sweden, and the UK, exhibited divergent subtype patterns between humans, animals, and environmental sources, whereas others, such as Spain and Germany, showed more consistent distributions. These findings highlight the importance of integrating clinical, veterinary, and environmental surveillance to better understand HEV transmission in Europe under a One Health framework. However, the scarcity of environmental data, technical challenges in sequencing, and lack of standardized protocols limit comprehensive assessment of HEV circulation. Expanding sequencing efforts, improving detection methods, and coordinating international surveillance frameworks will be critical to strengthen HEV monitoring and preparedness against emerging HEV threats.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101302"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knowledge, attitudes, and practices of women related to human and animal feces management in Ethiopia: Evidence for strengthening water, sanitation, and hygiene (WaSH) initiatives","authors":"Birhanu Hadush ,&nbsp;Chaltu Fikru ,&nbsp;Girma Kebede ,&nbsp;Syum Guesh ,&nbsp;Enquebaher Kassaye ,&nbsp;Getachew Redae ,&nbsp;Kebede Amenu ,&nbsp;Lisa Cavalerie ,&nbsp;Siobhan M. Mor","doi":"10.1016/j.onehlt.2025.101311","DOIUrl":"10.1016/j.onehlt.2025.101311","url":null,"abstract":"<div><h3>Background</h3><div>Water, sanitation, and hygiene (WaSH) programs aim to prevent feco-oral disease transmission, but their effectiveness in low- and middle-income countries is often compromised by frequent exposure to animal feces. In Ethiopia, national initiatives such as the Health Extension Program and Community-Led Total Sanitation and Hygiene program have expanded WaSH coverage but do not currently address animal-associated risks. This study investigated knowledge, attitudes, and practices related to human and animal feces management in Butajira, Gilgel Gibe, and Kilite Awlaelo Health and Demographic Surveillance Sites (HDSS) in Ethiopia.</div></div><div><h3>Methods</h3><div>A community-based, cross-sectional study was conducted through random sampling of households in selected kebeles across the three HDSS sites. Eligible households owned up to 60 livestock, including at least one head of cattle. Adult women (<em>n</em> = 1129) were interviewed using a structured questionnaire on WaSH and livestock husbandry, including manure management. Descriptive statistics were used to summarise practices across sites.</div></div><div><h3>Results</h3><div>The median age of participants ranged from 35 to 45 years, with female-headed households most frequent in Butajira (42.2 %). Literacy levels were lowest in Gilgel Gibe (64.1 % illiteracy). Most households accessed improved water sources, though unimproved sources were important in Gilgel Gibe (16.3 %). Improved toilet facilities were most common in Gilgel Gibe (61 %). At least 79 % of respondents reported handwashing after toilet use, with moderate soap use (&gt;73 %). Manure management was primarily the responsibility of adult women (&gt;74 %), often using bare hands. Handwashing with soap after manure handling was common in Kilite Awlaelo (&gt;97 %) but lower in Butajira (86.3 %) and Gilgel Gibe (74.8 %). Awareness of health risks from animal manure was lower than for human feces, especially in Gilgel Gibe (70 %) and Butajira (53.2 %). Similarly, awareness of the benefits of composting or drying manure to prevent germ spread was lower in these sites.</div></div><div><h3>Conclusions</h3><div>Despite high awareness of WaSH-related risks, critical gaps exist in understanding manure-associated health risks, particularly in Butajira and Gilgel Gibe. Targeted interventions are needed to raise awareness and improve manure management, especially among women.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101311"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Collaboration under pressure: sustaining One Health research teams in a post-COVID environment","authors":"Robin B. Gasser","doi":"10.1016/j.onehlt.2025.101298","DOIUrl":"10.1016/j.onehlt.2025.101298","url":null,"abstract":"<div><div>The sustainability of contemporary One Health research increasingly relies on the capacity of universities, research institutes and partner organisations to support collaborative teams. Such teams are indispensable because they integrate diverse expertise, address complex problems and respond rapidly to health crises such as COVID-19. One Health provides a compelling exemplar of collaborative research, uniting human, animal and environmental health to tackle global challenges and emerging infectious diseases. Yet the resilience of such teams is being tested by neoliberal reforms that have reshaped higher education into a competitive marketplace and by managerial practices that prioritise measurable outputs over collegiality, autonomy and disciplinary breadth. The pandemic exposed the fragility of this model: laboratories closed, workloads intensified and financial pressures triggered redundancies, even as collaboration proved essential to global health responses. This Opinion article examines how systemic pressures and interpersonal dynamics intersect to influence the functioning of research teams in a post-COVID environment. It highlights funding precarity, employment insecurity, inequities and political instability as persistent challenges, and explores how performance pressures and competition can affect collaboration. Sustaining research teams requires institutional reforms, inclusive leadership, recognition of diverse contributions and alignment with global frameworks such as the United Nations Sustainable Development Goals (SDGs), so that One Health research and other multidisciplinary endeavours addressing emerging and neglected diseases remain resilient, impactful and socially relevant.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101298"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic epidemiology of Salmonella Typhimurium and its monophasic variants in Southern China: A spatiotemporal and source attribution analysis","authors":"Ningbo Liao ,&nbsp;Shunxiong Lei ,&nbsp;Chengwei Liu ,&nbsp;Shengnan Tang ,&nbsp;Silu Peng","doi":"10.1016/j.onehlt.2025.101299","DOIUrl":"10.1016/j.onehlt.2025.101299","url":null,"abstract":"<div><div><em>Salmonella Typhimurium</em> and its monophasic variants are major contributors to foodborne illnesses globally, with zoonotic transmission posing significant public health risks. In southern China, persistent salmonellosis cases linked to poultry and pork highlight the need for advanced genomic tools to trace contamination sources and understand transmission dynamics. This study integrates whole-genome sequencing (WGS) and spatiotemporal data to investigate the molecular epidemiology of Salmonella in Jiangxi Province, a region with high incidence of foodborne salmonellosis. Analysis of 206 Salmonella isolates (2015–2021) revealed dominant sequence types (ST34, ST19, ST155, and ST469) associated with human clinical cases and food sources. High-resolution single nucleotide polymorphism (SNP) phylogenetic analysis revealed well-supported, monophasic clades corresponding to the major sequence types. This analysis provided strong genomic evidence for zoonotic transmission, with human clinical isolates being genetically almost identical (≤5 SNPs) to isolates from poultry (ST34/ST19) and pork (ST155/ST469) sources. Clonal clusters of monophasic Typhimurium variants (77.9 % of ST34 isolates) exhibited widespread geographic distribution across 11 prefectures, and the high genetic similarity among isolates suggests potential cross-regional transmission through contaminated food supply chains. High antimicrobial resistance (AMR) rates were detected against ampicillin (68.0 %), tetracycline (61.0 %), and sulfamethoxazole-trimethoprim (54.0 %), with multidrug-resistant (MDR) strains (60.2 %) prevalent in clinical and food-derived isolates. ST34 exhibited the highest MDR prevalence (75.4 %), driven by the presence of Salmonella Genomic Island 1 (SGI1) in many isolates. The β-lactamase gene blaTEM-1 was most prevalent (60.7 %), followed by tet(A) (54.4 %), and sul2 (47.6 %). Point mutations in the quinolone resistance-determining region (QRDR), specifically in gyrA, were identified as the primary mechanism for ciprofloxacin resistance. Spatial clustering identified significant clusters in live poultry markets, slaughterhouses, and retail meat, emphasizing the role of foodborne zoonotic reservoirs. Findings advocate for strengthened One Health interventions, including enhanced AMR monitoring, targeted food safety regulations, and real-time WGS-based surveillance to mitigate zoonotic transmission risks in southern China.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101299"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Widespread circulation of Crimean-Congo haemorrhagic fever virus in ticks, Corsica (France), 2024","authors":"Morena Gasparine ,&nbsp;Armand Namekong Fokeng ,&nbsp;Shirley Masse ,&nbsp;Eva Lopez ,&nbsp;Remi Charrel ,&nbsp;Xavier de Lamballerie ,&nbsp;Alessandra Falchi","doi":"10.1016/j.onehlt.2026.101339","DOIUrl":"10.1016/j.onehlt.2026.101339","url":null,"abstract":"<div><div>Crimean-Congo Haemorrhagic Fever is a severe tick-borne viral disease with a high fatality rate. This study aimed to advance the understanding of CCHF virus (CCHFV) in terms of geographical spread and genotypic characterization by investigating its detection in ticks collected from cattle, one year after the first detection of CCHFV in Corsica. From 2024 to 2025, we collected ticks from cattle, with standardised sampling occurring twice per month. Ticks were screened for CCHFV RNA by RTq-PCR. Genome sequencing and phylogenetic analyses were performed. Blood samples from a subset of these cattle were tested for CCHFV antibodies using a commercial enzyme-linked immunosorbent (ELISA) test. Among the 13,577 ticks collected, CCHFV RNA was detected in 61 pools (1.6%) out of the 3803 tested. We identified CCHFV African genotype III in ticks collected from cattle at different sites in northern and southern Corsica. However, two tick strains showed a reassortant profile, with the S and L segments belonging to genotype III and the M segment belonging to genotype I. Data also showed that our strains clustered with strains isolated in African and Western European countries. The overall IgG anti-CCHFV seroprevalence in cattle was 8.44% [95% CI: 6.27% - 11.06%]. This study provides new insights into the spatial and temporal distribution of CCHFV in Corsica and confirms the wider-than-expected distribution and variability of CCHFV in Corsica. Therefore, our findings confirm the genetic variability inside the CCHFV genotypes and their introduction to Corsica from other countries.</div></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":"22 ","pages":"Article 101339"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}