Abstract In the present study, some 2- and 3-substituted piperidin-4-ones (A1-A3) were chosen as proton acceptor and maleic anhydride was chosen as proton donor. Piperidin-4-ones (A1, A2 ---amp--- A3) were mixed with maleic anhydride in ether medium and the corresponding molecular adduct products (B1, B2 ---amp--- B3) thus obtained were collected and purified. The 1H and 13C NMR spectra were recorded for piperidine-4-one and their addition compounds. The 1H and 13C NMR chemical shifts of products (B1, B2 ---amp--- B3) are analyzed and compared with those of the corresponding piperidine-4-ones (A1, A2 ---amp--- A3). In order to confirm the formation of molecular addition compounds the GC-Mass spectrum was recorded for all adducts B1-B3 and the fragmentations patterns were analyzed.
{"title":"Spectral investigations of some piperidin-4-one molecular addition compounds","authors":"J. Chakkaravarthy, I. Muthuvel, G. Thirunarayanan","doi":"10.2478/auoc-2020-0005","DOIUrl":"https://doi.org/10.2478/auoc-2020-0005","url":null,"abstract":"Abstract In the present study, some 2- and 3-substituted piperidin-4-ones (A1-A3) were chosen as proton acceptor and maleic anhydride was chosen as proton donor. Piperidin-4-ones (A1, A2 ---amp--- A3) were mixed with maleic anhydride in ether medium and the corresponding molecular adduct products (B1, B2 ---amp--- B3) thus obtained were collected and purified. The 1H and 13C NMR spectra were recorded for piperidine-4-one and their addition compounds. The 1H and 13C NMR chemical shifts of products (B1, B2 ---amp--- B3) are analyzed and compared with those of the corresponding piperidine-4-ones (A1, A2 ---amp--- A3). In order to confirm the formation of molecular addition compounds the GC-Mass spectrum was recorded for all adducts B1-B3 and the fragmentations patterns were analyzed.","PeriodicalId":19641,"journal":{"name":"Ovidius University Annals of Chemistry","volume":"35 1","pages":"18 - 26"},"PeriodicalIF":0.9,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90926644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Doiniţa-Roxana Cioroiu Tirpan, C. Koncsag, T. Dobre
Abstract Cellulose fibres are known for their good mechanical properties, therefore they are used as fillers in structural composite materials, including as nanofibrils in nanomaterials. Also, they are biocompatible, non-toxic and biodegradable, reason for their use in the food industry as packaging materials or in obtaining medical materials. One source of cheap, easy- to- extract cellulose is the algal mass of Ulva lactuca, one of the most frequent species found in the Black Sea. In this study, cellulose extraction from Ulva lactuca was achieved by a simple low cost physical-chemical treatment. Freshly harvested seaweed was dried at 45 °C for 48 hours, transformed into a fine powder in order to increase the contact surface between the solvents and the alga. Extraction of lipids and chlorophyll took place in Soxhlet apparatus with ethanol. Successive steps of chemical treatment, having in view removal of hydrosoluble ulvans, pigments and hemicellulose lead to a yield of 15.36% in dry matter (DM) of cellulose-rich insoluble fraction proving that Ulva Lactuca species is a viable alternative resource in cellulose production.
{"title":"Cellulose fibers extraction from Ulva lactuca from the Black Sea","authors":"Doiniţa-Roxana Cioroiu Tirpan, C. Koncsag, T. Dobre","doi":"10.2478/AUOC-2020-0025","DOIUrl":"https://doi.org/10.2478/AUOC-2020-0025","url":null,"abstract":"Abstract Cellulose fibres are known for their good mechanical properties, therefore they are used as fillers in structural composite materials, including as nanofibrils in nanomaterials. Also, they are biocompatible, non-toxic and biodegradable, reason for their use in the food industry as packaging materials or in obtaining medical materials. One source of cheap, easy- to- extract cellulose is the algal mass of Ulva lactuca, one of the most frequent species found in the Black Sea. In this study, cellulose extraction from Ulva lactuca was achieved by a simple low cost physical-chemical treatment. Freshly harvested seaweed was dried at 45 °C for 48 hours, transformed into a fine powder in order to increase the contact surface between the solvents and the alga. Extraction of lipids and chlorophyll took place in Soxhlet apparatus with ethanol. Successive steps of chemical treatment, having in view removal of hydrosoluble ulvans, pigments and hemicellulose lead to a yield of 15.36% in dry matter (DM) of cellulose-rich insoluble fraction proving that Ulva Lactuca species is a viable alternative resource in cellulose production.","PeriodicalId":19641,"journal":{"name":"Ovidius University Annals of Chemistry","volume":"10 1","pages":"158 - 162"},"PeriodicalIF":0.9,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88145296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract In this study, we attempt to verify the claim that the leaf-extract of Telfairia occidentalis can remedy oxidative damage condition as well as assess its phytochemical content. Fifteen male albino rats weighing 180 g to 240 g were randomly divided into three groups of five rats each. Group A was designated the control group while group B and C were both induced with 40 mg/kg body weight 2,4-dinitrophenyl hydrazine. Group C was subsequently treated with 200 mg/kg body weight of ethanol extract of T. occidentalis leaf for 21 days. At the end of the treatment, the animals were sacrificed, and serum of the samples were subjected to relevant tests. Result shows that the plant leaf contained saponin, tannins, alkaloids, flavonoids and phenols whereas, terpenes, steroids and anthraquinones were not detected. The serum enzymes alanine aminotransferase (ALT) and alkaline phosphatase (ALP) were significantly elevated from 17.43 u/L and 28.40 u/L to 21.60 u/L and 34.27 u/L respectively. These were significantly lowered in the group C to 18.37 u/L and 29.23 u/L respectively for ALT and ALP. Also, a significant lowering of superoxide dismutase (SOD) activity was observed in the treated group (54.33 u/mg) from 79.40 u/mg recorded in the intoxicated group. Similarly, a significant decrease in malondialdehyde was observed in the treated group (25.80 u/mg) relative to the intoxicated group (35.87 u/mg). Moreover, catalase activity in the treated group (7.43 u/mg) was significantly lower compared with the intoxicated group. Our observation confirmed that ethanolic leaf extract of T. occidentalis reversed the oxidative damage condition in albino rats. The result confirms the ethnomedicinal use of the plant in the management of oxidative stress related diseases.
{"title":"Effect of ethanol extract of Telfairia occidentalis leaf on some biochemical parameters of 2,4-dinitrophenyl hydrazine induced oxidative stress in albino rats","authors":"O. E. Seyifunmi, A. Ajayi","doi":"10.2478/auoc-2020-0019","DOIUrl":"https://doi.org/10.2478/auoc-2020-0019","url":null,"abstract":"Abstract In this study, we attempt to verify the claim that the leaf-extract of Telfairia occidentalis can remedy oxidative damage condition as well as assess its phytochemical content. Fifteen male albino rats weighing 180 g to 240 g were randomly divided into three groups of five rats each. Group A was designated the control group while group B and C were both induced with 40 mg/kg body weight 2,4-dinitrophenyl hydrazine. Group C was subsequently treated with 200 mg/kg body weight of ethanol extract of T. occidentalis leaf for 21 days. At the end of the treatment, the animals were sacrificed, and serum of the samples were subjected to relevant tests. Result shows that the plant leaf contained saponin, tannins, alkaloids, flavonoids and phenols whereas, terpenes, steroids and anthraquinones were not detected. The serum enzymes alanine aminotransferase (ALT) and alkaline phosphatase (ALP) were significantly elevated from 17.43 u/L and 28.40 u/L to 21.60 u/L and 34.27 u/L respectively. These were significantly lowered in the group C to 18.37 u/L and 29.23 u/L respectively for ALT and ALP. Also, a significant lowering of superoxide dismutase (SOD) activity was observed in the treated group (54.33 u/mg) from 79.40 u/mg recorded in the intoxicated group. Similarly, a significant decrease in malondialdehyde was observed in the treated group (25.80 u/mg) relative to the intoxicated group (35.87 u/mg). Moreover, catalase activity in the treated group (7.43 u/mg) was significantly lower compared with the intoxicated group. Our observation confirmed that ethanolic leaf extract of T. occidentalis reversed the oxidative damage condition in albino rats. The result confirms the ethnomedicinal use of the plant in the management of oxidative stress related diseases.","PeriodicalId":19641,"journal":{"name":"Ovidius University Annals of Chemistry","volume":"15 1","pages":"106 - 109"},"PeriodicalIF":0.9,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87146909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Chromatographic separation of methanolic extract of Baphia nitida heartwood gave two crystalline solids characterized as 3,9-dimethoxy-6aR,11aR-dihydro-6H-benzofuro(3,2-C)[1]benzopyran (also known as homopterocarpin) with molecular formula C17H16O4 (1.57% yield) and 2,4-dimethoxybenzaldehyde C9H10O3 (2.27% yield). Each of the isolated compounds showed a single spot on developed thin layer chromatographic plate under ultra-violet light (254 nm) and spray reagent (10% sulfuric acid in methanol solution). Structural elucidation was achieved using Fourier transform infrared (FT-IR) spectroscopy, one and two-dimension nuclear magnetic resonance (NMR) techniques. Distortionless enhancement by polarization transfer-edited-heteronuclear single quantum coherence (DEPT-ed-HSQC) was also a useful tool that aided the characterization of the two secondary metabolites isolated from Baphia nitida heartwood.
{"title":"Isolation of an isoflavonoid and a terpenoid from the heartwood of Baphia nitida Lodd. (camwood)","authors":"G. Ndukwe, A. Oluah, G. K. Fekarurhobo","doi":"10.2478/auoc-2020-0002","DOIUrl":"https://doi.org/10.2478/auoc-2020-0002","url":null,"abstract":"Abstract Chromatographic separation of methanolic extract of Baphia nitida heartwood gave two crystalline solids characterized as 3,9-dimethoxy-6aR,11aR-dihydro-6H-benzofuro(3,2-C)[1]benzopyran (also known as homopterocarpin) with molecular formula C17H16O4 (1.57% yield) and 2,4-dimethoxybenzaldehyde C9H10O3 (2.27% yield). Each of the isolated compounds showed a single spot on developed thin layer chromatographic plate under ultra-violet light (254 nm) and spray reagent (10% sulfuric acid in methanol solution). Structural elucidation was achieved using Fourier transform infrared (FT-IR) spectroscopy, one and two-dimension nuclear magnetic resonance (NMR) techniques. Distortionless enhancement by polarization transfer-edited-heteronuclear single quantum coherence (DEPT-ed-HSQC) was also a useful tool that aided the characterization of the two secondary metabolites isolated from Baphia nitida heartwood.","PeriodicalId":19641,"journal":{"name":"Ovidius University Annals of Chemistry","volume":"28 1","pages":"5 - 8"},"PeriodicalIF":0.9,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85750455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Confectionery industry represents a field that uses a large number of ingredients and techniques to develop unique sweet products. To produce aerated confectionery samples two different procedures were used to incorporate the ingredients in the beating vegetable or dairy cream. The objective of this research was to determine the texture parameters and the viscoelastic properties of aerated confections using compression stress-relaxation test and applying a modified Maxwell model. The highest fat content was presented by dairy cream aerated samples (20.04-20.25%), while the samples based on vegetable cream displayed a lower fat content. By applying the modified Maxwell mechanical model to the relaxation curves the equilibrium stress, σe, relaxation time, λrel, viscosity, η, and modulus of elasticity, G0, were determined. The aerated samples’ viscosity was greater than 137.96 kPa·s and less than 451.793 kPa·s; furthermore, Pearson correlation showed that density influences positively this rheological parameter (r = 0.955*). Fixing air into the product structure causes a decrease in density (0.388-0.788 g/cm3), leading to a lower equilibrium stress, a lower elasticity modulus and also a decrease of viscosity and relaxation time.
{"title":"Texture and rheological evaluation of aerated confectionery","authors":"Sergiu Pădureț, R. Zimbru, Sonia Amariei","doi":"10.2478/auoc-2020-0012","DOIUrl":"https://doi.org/10.2478/auoc-2020-0012","url":null,"abstract":"Abstract Confectionery industry represents a field that uses a large number of ingredients and techniques to develop unique sweet products. To produce aerated confectionery samples two different procedures were used to incorporate the ingredients in the beating vegetable or dairy cream. The objective of this research was to determine the texture parameters and the viscoelastic properties of aerated confections using compression stress-relaxation test and applying a modified Maxwell model. The highest fat content was presented by dairy cream aerated samples (20.04-20.25%), while the samples based on vegetable cream displayed a lower fat content. By applying the modified Maxwell mechanical model to the relaxation curves the equilibrium stress, σe, relaxation time, λrel, viscosity, η, and modulus of elasticity, G0, were determined. The aerated samples’ viscosity was greater than 137.96 kPa·s and less than 451.793 kPa·s; furthermore, Pearson correlation showed that density influences positively this rheological parameter (r = 0.955*). Fixing air into the product structure causes a decrease in density (0.388-0.788 g/cm3), leading to a lower equilibrium stress, a lower elasticity modulus and also a decrease of viscosity and relaxation time.","PeriodicalId":19641,"journal":{"name":"Ovidius University Annals of Chemistry","volume":"90 1","pages":"60 - 65"},"PeriodicalIF":0.9,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83456519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Suresh, S. P. Sakthinathan, D. Kamalakkannan, I. Muthuvel, G. Thirunarayanan
Abstract A number of nine benzylidene-4-nitroanilines were synthesized by condensation method. The formation of the substituted (E)-N-benzylidene-4-nitrobenzenamines has been confirmed from their physical and Ultra-Violet, Infra-Red, NMR spectral data. The evaluation of antimicrobial screening of substituted (E)-N-benzylidene-4-nitrobenzenamines was conducted by using standard Bauer-Kirby method. Three gram-positive microbes namely Bacillus subtilis, Micrococcus luteus and Staphylococcus aureus, and two gram-negative microbes, Escherichia coli and Pseudomonas aeruginosa, were used for the antibacterial evaluation. The antifungal activities against Aspergillus niger and Penicilium scup fungal species were also performed. A good antibacterial effect has been possessed by some of the substituted (E)-N-benzylidene-4-nitrobenzenamines on the microorganisms utilized in the present study
摘要采用缩合法合成了9种苄基-4-硝基苯胺。取代的(E)- n -苄基-4-硝基苯胺的形成已通过它们的物理和紫外、红外、核磁共振光谱数据得到证实。采用标准Bauer-Kirby法评价取代(E)- n -苄基-4-硝基苯胺的抗菌筛选效果。采用枯草芽孢杆菌、黄体微球菌、金黄色葡萄球菌3种革兰氏阳性菌和大肠杆菌、铜绿假单胞菌2种革兰氏阴性菌进行抗菌评价。并对黑曲霉和青霉进行了抑菌试验。一些取代的(E)- n -苄基-4-硝基苯胺对微生物具有良好的抑菌效果
{"title":"Synthesis and pharmacological evaluation of some benzylidene-4-nitroanilines","authors":"R. Suresh, S. P. Sakthinathan, D. Kamalakkannan, I. Muthuvel, G. Thirunarayanan","doi":"10.2478/auoc-2020-0011","DOIUrl":"https://doi.org/10.2478/auoc-2020-0011","url":null,"abstract":"Abstract A number of nine benzylidene-4-nitroanilines were synthesized by condensation method. The formation of the substituted (E)-N-benzylidene-4-nitrobenzenamines has been confirmed from their physical and Ultra-Violet, Infra-Red, NMR spectral data. The evaluation of antimicrobial screening of substituted (E)-N-benzylidene-4-nitrobenzenamines was conducted by using standard Bauer-Kirby method. Three gram-positive microbes namely Bacillus subtilis, Micrococcus luteus and Staphylococcus aureus, and two gram-negative microbes, Escherichia coli and Pseudomonas aeruginosa, were used for the antibacterial evaluation. The antifungal activities against Aspergillus niger and Penicilium scup fungal species were also performed. A good antibacterial effect has been possessed by some of the substituted (E)-N-benzylidene-4-nitrobenzenamines on the microorganisms utilized in the present study","PeriodicalId":19641,"journal":{"name":"Ovidius University Annals of Chemistry","volume":"58 1","pages":"55 - 59"},"PeriodicalIF":0.9,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88395512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Mala, I. Muthuvel, G. Thirunarayanan, S. P. Sakthinathan, R. Arulkumaran, Venkatesan Mani, R. Sundararajan, D. Kamalakkannan, R. Suresh, Veeramalai Usha
Abstract More than 85% yield of (E)-1,2,3-triphenylprop-2-en-1-ones were synthesized using disodium hydrogen phosphate (Na2HPO4) catalyzed ultrasound assisted aldol condensation of 1,2-diphenylethanone and various substituted benzaldehydes. Synthesized (E)-1,2,3-triphenylprop-2-en-1-ones were examined by their spectroscopic data, yield, micro analysis and physical constants. The effect of solvent on the yield was investigated. The pharmacological effects such as antibacterial and antifungal activities of synthesized enones were evaluated with Bauer-Kirby disc diffusion method.
{"title":"Ultrasound assisted synthesis and pharmacological evaluation of some (E)-1,2,3-triphenylprop-2-en-1-ones","authors":"V. Mala, I. Muthuvel, G. Thirunarayanan, S. P. Sakthinathan, R. Arulkumaran, Venkatesan Mani, R. Sundararajan, D. Kamalakkannan, R. Suresh, Veeramalai Usha","doi":"10.2478/AUOC-2020-0024","DOIUrl":"https://doi.org/10.2478/AUOC-2020-0024","url":null,"abstract":"Abstract More than 85% yield of (E)-1,2,3-triphenylprop-2-en-1-ones were synthesized using disodium hydrogen phosphate (Na2HPO4) catalyzed ultrasound assisted aldol condensation of 1,2-diphenylethanone and various substituted benzaldehydes. Synthesized (E)-1,2,3-triphenylprop-2-en-1-ones were examined by their spectroscopic data, yield, micro analysis and physical constants. The effect of solvent on the yield was investigated. The pharmacological effects such as antibacterial and antifungal activities of synthesized enones were evaluated with Bauer-Kirby disc diffusion method.","PeriodicalId":19641,"journal":{"name":"Ovidius University Annals of Chemistry","volume":"14 1","pages":"152 - 157"},"PeriodicalIF":0.9,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86678189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract The present study uses numerous chemometric techniques to evaluate and interpret a water quality data obtained from the drinking water resources namely municipal water (supplied by Rajghat dam on Bewas River), bore well, ground water of Sagar city, a divisional headquarter of Madhya Pradesh, India. Data was collected from May 2018 to June 2019 for 10 parameters used to assess the status of the water quality. Water quality was monitored at 15 sampling stations along the entire district. The data were analyzed using chemometric analysis such as principal component analysis, correlation matrix, multivariate linear regression analysis and hierarchical cluster analysis that reduced the data dimensions for better interpretation. Results of statistical analysis expressed that slightly higher value of BOD in some areas due to sewage contamination, need of chlorination treatment required at those places. This study also presents the value of diverse statistical methods for assessment and analysis of drinking water quality data for the reason of monitoring the effectiveness of water resource management. The study indicated that the maximum quality parameters of drinking water is in permissible limits of WHO and IS: 10500 guidelines on entire study places.
{"title":"Chemometric analysis of drinking water quality parameters of Sagar city, Madhya Pradesh, India","authors":"H. Pathak","doi":"10.2478/auoc-2020-0018","DOIUrl":"https://doi.org/10.2478/auoc-2020-0018","url":null,"abstract":"Abstract The present study uses numerous chemometric techniques to evaluate and interpret a water quality data obtained from the drinking water resources namely municipal water (supplied by Rajghat dam on Bewas River), bore well, ground water of Sagar city, a divisional headquarter of Madhya Pradesh, India. Data was collected from May 2018 to June 2019 for 10 parameters used to assess the status of the water quality. Water quality was monitored at 15 sampling stations along the entire district. The data were analyzed using chemometric analysis such as principal component analysis, correlation matrix, multivariate linear regression analysis and hierarchical cluster analysis that reduced the data dimensions for better interpretation. Results of statistical analysis expressed that slightly higher value of BOD in some areas due to sewage contamination, need of chlorination treatment required at those places. This study also presents the value of diverse statistical methods for assessment and analysis of drinking water quality data for the reason of monitoring the effectiveness of water resource management. The study indicated that the maximum quality parameters of drinking water is in permissible limits of WHO and IS: 10500 guidelines on entire study places.","PeriodicalId":19641,"journal":{"name":"Ovidius University Annals of Chemistry","volume":"40 1","pages":"99 - 105"},"PeriodicalIF":0.9,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89669388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Omoregie, A. Ogofure, B. Ikhajiagbe, G. O. Anoliefo
Abstract The presence and impact of bulk and rhizosphere microorganisms in contaminated soils can be huge, given that they have the ability to increase plants tolerance against abiotic stress, and also enhance plant growth, while supporting hastened remediation of disturbed soils. The present study quantitatively and qualitatively assessed presence of cultural fungi and bacteria during phytoremediation of heavy metal polluted soils using Chromolaena odorata. Stem cuttings of C. odorata were planted in soils polluted with Pb, Mn, Zn, Cd, and Cu at once (1ESC), thrice (3ESC) and five (5ESC) times their respective ecological screening concentrations (ESC). ESC of Pb, Mn and Zn is 50 mg/kg, Cd is 4 mg/kg, and Cu is 100 mg/kg. After 6 months, results showed that more than 10 species of bacteria and fungi were identified in the study, with P. aeruginosa and Bacillus subtilis being the most occurring bacteria while, Penicillium sp. and Aspergillus niger the most occurring fungi in both bulk and rhizospheric soils. The presence of known plant growth promoting rhizobacteria in plants rhizosphere including Azotobacter sp., Bacillus subtilis, B. pumilus, Clostridium sp., P. aeruginosa, and Klebsiella sp. was also reported.
{"title":"Quantitative and qualitative basement of microbial presence during phytoremediation of heavy metal polluted soil using Chromolaena odorata","authors":"G. Omoregie, A. Ogofure, B. Ikhajiagbe, G. O. Anoliefo","doi":"10.2478/AUOC-2020-0023","DOIUrl":"https://doi.org/10.2478/AUOC-2020-0023","url":null,"abstract":"Abstract The presence and impact of bulk and rhizosphere microorganisms in contaminated soils can be huge, given that they have the ability to increase plants tolerance against abiotic stress, and also enhance plant growth, while supporting hastened remediation of disturbed soils. The present study quantitatively and qualitatively assessed presence of cultural fungi and bacteria during phytoremediation of heavy metal polluted soils using Chromolaena odorata. Stem cuttings of C. odorata were planted in soils polluted with Pb, Mn, Zn, Cd, and Cu at once (1ESC), thrice (3ESC) and five (5ESC) times their respective ecological screening concentrations (ESC). ESC of Pb, Mn and Zn is 50 mg/kg, Cd is 4 mg/kg, and Cu is 100 mg/kg. After 6 months, results showed that more than 10 species of bacteria and fungi were identified in the study, with P. aeruginosa and Bacillus subtilis being the most occurring bacteria while, Penicillium sp. and Aspergillus niger the most occurring fungi in both bulk and rhizospheric soils. The presence of known plant growth promoting rhizobacteria in plants rhizosphere including Azotobacter sp., Bacillus subtilis, B. pumilus, Clostridium sp., P. aeruginosa, and Klebsiella sp. was also reported.","PeriodicalId":19641,"journal":{"name":"Ovidius University Annals of Chemistry","volume":"90 1","pages":"145 - 151"},"PeriodicalIF":0.9,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81133708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract The present paper presents our attempts concerning the development of an extraction method for catecholamines. In order to achieve the extraction of all the selected solutes using a single SPE cartridge, several types of support were tested, among them: cation exchange supports, hydrophilic-lipophilic supports, C18 supports and PGC supports. As unfortunately none of the supports tested offered us the possibility of carrying out the extraction of 12 catecholamines from our standard mixture, we chose to use a coupling of two different cartridges: Oasis HLB and PGC which together ensure the extraction of all the compounds of the mixture with good extraction yields and with simple protocols. The selected cartridges were successfully tested for the extraction of a sample spiked from sheep brain with the 12 catecholamines in our mixture. The SPE method that we have developed allows the purification of the samples (a significant part of the components of the matrix is eliminated during this step) and also a preconcentration of the samples.
{"title":"Optimization of SPE method for the extraction of 12 neurotransmitters from sheep brain","authors":"Raluca Tampu, C. Tâmpu, C. Elfakir","doi":"10.2478/auoc-2020-0020","DOIUrl":"https://doi.org/10.2478/auoc-2020-0020","url":null,"abstract":"Abstract The present paper presents our attempts concerning the development of an extraction method for catecholamines. In order to achieve the extraction of all the selected solutes using a single SPE cartridge, several types of support were tested, among them: cation exchange supports, hydrophilic-lipophilic supports, C18 supports and PGC supports. As unfortunately none of the supports tested offered us the possibility of carrying out the extraction of 12 catecholamines from our standard mixture, we chose to use a coupling of two different cartridges: Oasis HLB and PGC which together ensure the extraction of all the compounds of the mixture with good extraction yields and with simple protocols. The selected cartridges were successfully tested for the extraction of a sample spiked from sheep brain with the 12 catecholamines in our mixture. The SPE method that we have developed allows the purification of the samples (a significant part of the components of the matrix is eliminated during this step) and also a preconcentration of the samples.","PeriodicalId":19641,"journal":{"name":"Ovidius University Annals of Chemistry","volume":"57 1","pages":"110 - 121"},"PeriodicalIF":0.9,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74171666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：