Pub Date : 2023-01-01DOI: 10.29090/psa.2023.03.23.492
A. R. Putri, W. Setyaningsih, Ceferino Carrera Fernández, M. Palma, A. Rohman, S. Riyanto
Patin ( Pangasius micronemus ) fish oil is rich in omega-3 fatty acids such as α-linolenic acid and eicosapentaenoic. To obtain the benefits of omega-3, it is necessary to develop an efficient extraction method for patin oil (PO). This study aimed to develop the microwave-assisted extraction (MAE) method for fish oil from patin. Three independent variables with three levels were priorly evaluated using Box–Behnken design, including temperature (30, 60, 90°C), solvent composition (30, 60, 90% of ethyl acetate in methanol), and solvent to sample ratio (10:1, 15:1, 20:1). The significant variables were then optimized by response surface methodology (RSM). A second-order quadratic model of RSM suggested an extraction at 60°C with 56% ethyl acetate in methanol as an extraction solvent and a solvent-to-sample ratio of 20:1. A kinetics study under the optimum MAE conditions approved the complete recovery (38.84%) starting at 15 min of extraction time. The high precision of the MAE process was confirmed by the coefficient of variation less than 3%. Additionally, the microwave-produced fish oil was characterized by gas chromatography-mass spectrometry (GC-MS) to contain α-linolenic acid and eicosapentaenoic as omega-3. Henceforth, it has been demonstrated that microwave-assisted fish oil extraction developed in this study is efficient for high-quality PO production
{"title":"Optimization of microwave-assisted fish oil extraction from Patin (Pangasius micronemus) using Response Surface Methodology-Box Behnken Design (RSM-BBD)","authors":"A. R. Putri, W. Setyaningsih, Ceferino Carrera Fernández, M. Palma, A. Rohman, S. Riyanto","doi":"10.29090/psa.2023.03.23.492","DOIUrl":"https://doi.org/10.29090/psa.2023.03.23.492","url":null,"abstract":"Patin ( Pangasius micronemus ) fish oil is rich in omega-3 fatty acids such as α-linolenic acid and eicosapentaenoic. To obtain the benefits of omega-3, it is necessary to develop an efficient extraction method for patin oil (PO). This study aimed to develop the microwave-assisted extraction (MAE) method for fish oil from patin. Three independent variables with three levels were priorly evaluated using Box–Behnken design, including temperature (30, 60, 90°C), solvent composition (30, 60, 90% of ethyl acetate in methanol), and solvent to sample ratio (10:1, 15:1, 20:1). The significant variables were then optimized by response surface methodology (RSM). A second-order quadratic model of RSM suggested an extraction at 60°C with 56% ethyl acetate in methanol as an extraction solvent and a solvent-to-sample ratio of 20:1. A kinetics study under the optimum MAE conditions approved the complete recovery (38.84%) starting at 15 min of extraction time. The high precision of the MAE process was confirmed by the coefficient of variation less than 3%. Additionally, the microwave-produced fish oil was characterized by gas chromatography-mass spectrometry (GC-MS) to contain α-linolenic acid and eicosapentaenoic as omega-3. Henceforth, it has been demonstrated that microwave-assisted fish oil extraction developed in this study is efficient for high-quality PO production","PeriodicalId":19761,"journal":{"name":"Pharmaceutical Sciences Asia","volume":"104 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80511622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.29090/psa.2023.03.23.105
Nattanida Jantarach, Wirinya Mueangchang, Jatuporn Prathumtet, N. Munkong
Red rice bran extract (RRBE) has been demonstrated to have anti-lipid accumulation, antioxidant, and anti-inflammatory effects on the livers of obese animals and/or in macrophages. However, the molecular mechanisms driving these effects have not yet been investigated in brown adipose tissue (BAT). Thus, the present study aimed to investigate the beneficial effects of RRBE on BAT in mice with obesity induced by a high-fat diet (HFD). Six weeks after induction of obesity, the obese mice were fed continuously with HFD, with or without RRBE treatment for 6 weeks. Body weight and food intake were recorded weekly. At the end of the feeding experiment, BAT was collected for determination of expressions of genes associated with liporegulation, inflammation, and oxidative stress, as well as histological examination. Compared with untreated obese mice, RRBE-treated mice had reductions in body weight, food efficiency ratio, and white-like adipocytes in BAT, accompanied by down-regulated expression of nuclear receptor subfamily 1 group H member 3 (NR1H3). Moreover, RRBE decreased the levels of nitric oxide (NO), reactive oxygen species (ROS), and malondialdehyde (MDA) in BAT, which were associated with modulated expressions of target genes for nuclear factor-kappa B and nuclear factor erythroid 2-related factor 2. RRBE produced beneficial effects against HFD-induced abnormalities in BAT by mitigating whitening, inflammation, and oxidative stress.
{"title":"Beneficial effects of red rice bran extract on brown adipose tissue whitening, inflammation, and oxidative stress in mice with obesity induced by a high-fat diet","authors":"Nattanida Jantarach, Wirinya Mueangchang, Jatuporn Prathumtet, N. Munkong","doi":"10.29090/psa.2023.03.23.105","DOIUrl":"https://doi.org/10.29090/psa.2023.03.23.105","url":null,"abstract":"Red rice bran extract (RRBE) has been demonstrated to have anti-lipid accumulation, antioxidant, and anti-inflammatory effects on the livers of obese animals and/or in macrophages. However, the molecular mechanisms driving these effects have not yet been investigated in brown adipose tissue (BAT). Thus, the present study aimed to investigate the beneficial effects of RRBE on BAT in mice with obesity induced by a high-fat diet (HFD). Six weeks after induction of obesity, the obese mice were fed continuously with HFD, with or without RRBE treatment for 6 weeks. Body weight and food intake were recorded weekly. At the end of the feeding experiment, BAT was collected for determination of expressions of genes associated with liporegulation, inflammation, and oxidative stress, as well as histological examination. Compared with untreated obese mice, RRBE-treated mice had reductions in body weight, food efficiency ratio, and white-like adipocytes in BAT, accompanied by down-regulated expression of nuclear receptor subfamily 1 group H member 3 (NR1H3). Moreover, RRBE decreased the levels of nitric oxide (NO), reactive oxygen species (ROS), and malondialdehyde (MDA) in BAT, which were associated with modulated expressions of target genes for nuclear factor-kappa B and nuclear factor erythroid 2-related factor 2. RRBE produced beneficial effects against HFD-induced abnormalities in BAT by mitigating whitening, inflammation, and oxidative stress.","PeriodicalId":19761,"journal":{"name":"Pharmaceutical Sciences Asia","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90059753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.29090/psa.2023.01.22.160
Sandeep Kumar, Govind Singh
{"title":"Effect of zonisamide and Nigella sativa on blood-brain barrier permeability and neurological severity in traumatic brain injury-induced mice","authors":"Sandeep Kumar, Govind Singh","doi":"10.29090/psa.2023.01.22.160","DOIUrl":"https://doi.org/10.29090/psa.2023.01.22.160","url":null,"abstract":"","PeriodicalId":19761,"journal":{"name":"Pharmaceutical Sciences Asia","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76337597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.29090/psa.2023.02.22.368
Suwida Tangtrakultham, Jantana Houngsaitong, K. Sathirakul, Wichit Nosoongnoen, S. Chumnumwat, P. Montakantikul
{"title":"Optimal dosing regimen of biapenem and fosfomycin sodium combination against multidrug resistant Acinetobacter baumannii infection in pediatric patients using Monte Carlo simulation","authors":"Suwida Tangtrakultham, Jantana Houngsaitong, K. Sathirakul, Wichit Nosoongnoen, S. Chumnumwat, P. Montakantikul","doi":"10.29090/psa.2023.02.22.368","DOIUrl":"https://doi.org/10.29090/psa.2023.02.22.368","url":null,"abstract":"","PeriodicalId":19761,"journal":{"name":"Pharmaceutical Sciences Asia","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77635077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.29090/psa.2023.03.22.187
Rakesh Bramhankar, A. K. Mishra, Snigdha Das, N. Munishwar
Herbo-bio-mineral metallic preparations such as Abhrasindoora offer advantages over plant drugs under their stability over a period, lower dosage, and contain minerals and metals as an integral part of the formulations. The use of metals in medicine is often associated with toxicity. Present study was conducted to explore the acute and sub-acute toxicity study of Abhrasindoora in Wistar albino rats. Information related to possible health hazards, the estimate of no-observe-adverse-effect-level (NOAEL), hematology, and biochemical and histopathology parameters were studied. For the acute toxicity study rats were divided into two groups: Group I (Test group: 300 mg/kg) and Group II (Confirmatory group: 2,000 mg/kg) as per OECD 423 guidelines and for subacute toxicity study, rats were divided into four groups as Group I (water as the vehicle), Group II (600 mg/kg), Group III (800 mg/kg) and Group IV (1,000 mg/kg) as per OECD 407 guidelines. Clinical signs (daily); body weight and feed consumption (weekly) were taken. Blood samples were collected from the retro-orbital sinus before the necropsy (29 th day). For statistical analysis, one-way ANOVA and Dunnett’s test were used. In comparison to the control group, no mortality or adverse effects were observed. No significant variations were observed in hematological and biochemical parameters. In target organs, no significant changes were observed in both acute and sub-acute toxicity studies. No deaths or any signs of toxicity were observed after oral administration in the acute toxicity study up to the dose of 2,000 mg/kg and in the sub-acute toxicity study up to the dose of 1,000 mg/kg.
{"title":"Evaluation of acute and subacute toxicity of Abhrasindoora: As a mercury-based traditional herbo-bio-mineral metallic formulation","authors":"Rakesh Bramhankar, A. K. Mishra, Snigdha Das, N. Munishwar","doi":"10.29090/psa.2023.03.22.187","DOIUrl":"https://doi.org/10.29090/psa.2023.03.22.187","url":null,"abstract":"Herbo-bio-mineral metallic preparations such as Abhrasindoora offer advantages over plant drugs under their stability over a period, lower dosage, and contain minerals and metals as an integral part of the formulations. The use of metals in medicine is often associated with toxicity. Present study was conducted to explore the acute and sub-acute toxicity study of Abhrasindoora in Wistar albino rats. Information related to possible health hazards, the estimate of no-observe-adverse-effect-level (NOAEL), hematology, and biochemical and histopathology parameters were studied. For the acute toxicity study rats were divided into two groups: Group I (Test group: 300 mg/kg) and Group II (Confirmatory group: 2,000 mg/kg) as per OECD 423 guidelines and for subacute toxicity study, rats were divided into four groups as Group I (water as the vehicle), Group II (600 mg/kg), Group III (800 mg/kg) and Group IV (1,000 mg/kg) as per OECD 407 guidelines. Clinical signs (daily); body weight and feed consumption (weekly) were taken. Blood samples were collected from the retro-orbital sinus before the necropsy (29 th day). For statistical analysis, one-way ANOVA and Dunnett’s test were used. In comparison to the control group, no mortality or adverse effects were observed. No significant variations were observed in hematological and biochemical parameters. In target organs, no significant changes were observed in both acute and sub-acute toxicity studies. No deaths or any signs of toxicity were observed after oral administration in the acute toxicity study up to the dose of 2,000 mg/kg and in the sub-acute toxicity study up to the dose of 1,000 mg/kg.","PeriodicalId":19761,"journal":{"name":"Pharmaceutical Sciences Asia","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90323625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.29090/psa.2023.02.22.326
Sheila Mantaring, Debrah Jannsen DJ Almazan, Stephen Kyle Arcan, Nathalie Noval, Aracelle Palanca, J. P. Jose, Reneelyn Danganan-Cutab, J. Guzman
Pathogens form biofilms to increase their resistance to environmental stress and antibacterial compounds. The rhizosphere is a rich source of microorganisms producing industrially important compounds including those with antimicrobial and biofilm inhibitory activities. Four isolates from soil collected from Taguig City, Philippines, were subjected to phenotypic and genotypic characterization, screening for protease production, and biofilm inhibition assays. Colony morphology and microscopic analyses indicated the isolates were putative Bacillus species. Upon DNA extraction, 16S rRNA gene was amplified, and based on their sequences, the isolates were confirmed to be Bacillus spp. Isolate AHP was B. cereus , isolate DJA was Priestia megaterium , formerly known as B. megaterium and isolates SJS and SKA were Bacillus spp.—all of which produced protease. Although the cell-free supernatants (CFS) of the isolates did not inhibit the growth of Staphylococcus aureus 1258, Citrobacter freundii ATCC24864, Salmonella Typhimurium ATCC13311, Escherichia coli ATCC11229, and E. coli O157:H7, biofilm formation of S. aureus was inhibited by all CFS, with B. cereus AHP showing the highest biofilm inhibition at 46%, followed by Bacillus sp. SKA (39%), P. megaterium DJA (36%) , and Bacillus sp. SJS (31%). Even though further studies are warranted, the bioactivities of these isolates indicate potential use for pharmaceutical purposes due to their ability to produce protease and inhibition of biofilm formation of a common bacterial pathogen
{"title":"Locally-isolated protease-producing Bacillus spp. from soil inhibits biofilm formation of Staphylococcus aureus","authors":"Sheila Mantaring, Debrah Jannsen DJ Almazan, Stephen Kyle Arcan, Nathalie Noval, Aracelle Palanca, J. P. Jose, Reneelyn Danganan-Cutab, J. Guzman","doi":"10.29090/psa.2023.02.22.326","DOIUrl":"https://doi.org/10.29090/psa.2023.02.22.326","url":null,"abstract":"Pathogens form biofilms to increase their resistance to environmental stress and antibacterial compounds. The rhizosphere is a rich source of microorganisms producing industrially important compounds including those with antimicrobial and biofilm inhibitory activities. Four isolates from soil collected from Taguig City, Philippines, were subjected to phenotypic and genotypic characterization, screening for protease production, and biofilm inhibition assays. Colony morphology and microscopic analyses indicated the isolates were putative Bacillus species. Upon DNA extraction, 16S rRNA gene was amplified, and based on their sequences, the isolates were confirmed to be Bacillus spp. Isolate AHP was B. cereus , isolate DJA was Priestia megaterium , formerly known as B. megaterium and isolates SJS and SKA were Bacillus spp.—all of which produced protease. Although the cell-free supernatants (CFS) of the isolates did not inhibit the growth of Staphylococcus aureus 1258, Citrobacter freundii ATCC24864, Salmonella Typhimurium ATCC13311, Escherichia coli ATCC11229, and E. coli O157:H7, biofilm formation of S. aureus was inhibited by all CFS, with B. cereus AHP showing the highest biofilm inhibition at 46%, followed by Bacillus sp. SKA (39%), P. megaterium DJA (36%) , and Bacillus sp. SJS (31%). Even though further studies are warranted, the bioactivities of these isolates indicate potential use for pharmaceutical purposes due to their ability to produce protease and inhibition of biofilm formation of a common bacterial pathogen","PeriodicalId":19761,"journal":{"name":"Pharmaceutical Sciences Asia","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73899818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hypovitaminosis D (Vitamin D deficiency and insufficiency) is a major public health problem globally. A high prevalence of vitamin D deficiency (VDD) and vitamin D insufficiency (VDI) has been noted in South Asian (SA) women of childbearing age despite the abundant sunshine. Therefore, this study aimed to systematically review the existing data from population-based studies in this region to summate the magnitude of the problem. Articles published and reported prevalence of VDD/VDI in eight South Asian countries during period of 2010-2020 were searched and screened from PUBMED and Google Scholar using PRISMA guidelines. The study population was healthy women of childbearing age (18-45 years) and included pregnant and non-pregnant women. Fourteen population-based studies were found from SA countries India, Sri Lanka, Pakistan, Bangladesh and Nepal. No studies were available from Afghanistan, Bhutan, and Maldives pertaining to the searched period. Participant groups were from diverse socioeconomic and cultural backgrounds. The prevalence ranged from 61.5% from Pakistan to 100% from Bangladesh. This systematic review reported a significantly higher prevalence of hypo-vitaminosis D among SA women of childbearing age. Inadequate sun exposure, lack of consumption of good vitamin D food sources due to either illiteracy or economic constrains has been regarded as potential determinants of hypovitaminosis D among participants. The findings of this study would help pertinent stake holders to prepare suitable national level programs to enhance the Vitamin D status of women of childbearing age.
{"title":"Vitamin D status of healthy childbearing age women in South Asia: A pooled analysis of community-based studies","authors":"Siyumi Hasara Jayathilaka, Deepthi Inoka Uluwaduge","doi":"10.29090/psa.2023.02.22.354","DOIUrl":"https://doi.org/10.29090/psa.2023.02.22.354","url":null,"abstract":"Hypovitaminosis D (Vitamin D deficiency and insufficiency) is a major public health problem globally. A high prevalence of vitamin D deficiency (VDD) and vitamin D insufficiency (VDI) has been noted in South Asian (SA) women of childbearing age despite the abundant sunshine. Therefore, this study aimed to systematically review the existing data from population-based studies in this region to summate the magnitude of the problem. Articles published and reported prevalence of VDD/VDI in eight South Asian countries during period of 2010-2020 were searched and screened from PUBMED and Google Scholar using PRISMA guidelines. The study population was healthy women of childbearing age (18-45 years) and included pregnant and non-pregnant women. Fourteen population-based studies were found from SA countries India, Sri Lanka, Pakistan, Bangladesh and Nepal. No studies were available from Afghanistan, Bhutan, and Maldives pertaining to the searched period. Participant groups were from diverse socioeconomic and cultural backgrounds. The prevalence ranged from 61.5% from Pakistan to 100% from Bangladesh. This systematic review reported a significantly higher prevalence of hypo-vitaminosis D among SA women of childbearing age. Inadequate sun exposure, lack of consumption of good vitamin D food sources due to either illiteracy or economic constrains has been regarded as potential determinants of hypovitaminosis D among participants. The findings of this study would help pertinent stake holders to prepare suitable national level programs to enhance the Vitamin D status of women of childbearing age.","PeriodicalId":19761,"journal":{"name":"Pharmaceutical Sciences Asia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79022125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of rational drug use based on World Health Organization indicators in a tertiary hospital, Thailand","authors":"A. Khangtragool, Katemanee Nukompun, Anongnart Teeyasuntranonn, Phimwaree Wannasiri, Sittipong Moraray, Waristha Khangtragool","doi":"10.29090/psa.2023.01.22.317","DOIUrl":"https://doi.org/10.29090/psa.2023.01.22.317","url":null,"abstract":"","PeriodicalId":19761,"journal":{"name":"Pharmaceutical Sciences Asia","volume":"97 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88894112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.29090/psa.2023.03.23.377
Lubna Ali Abd Al-mutalib, A. Zgair
Biofilm formation of Pseudomonas aeruginosa ( P. aeruginosa ) and its adherence increases bacterial virulence to cause the infection. The role of treating P. aeruginosa with sub-inhibitory concentrations of ofloxacin in vitro in reducing bacterial adherence to biotic and abiotic surfaces was evaluated. Ten isolates of P. aeruginosa were isolated from urine samples. Biofilm formation on polystyrene microtiter plates and Minimum inhibitory concentrations (MICs) of ofloxacin against all isolates were evaluated. The effect of sub-MICs of ofloxacin (0.5×MIC, 0.25×MIC, 0.125×MIC, and 0.06×MIX) on biofilm formation (to polystyrene) and adhesion to prepared human epithelial cells (HECs) in vitro was evaluated. The MICs of ofloxacin were lower than 64 µg/ml and all isolates produced biofilm. There was no relationship between the susceptibility of bacterial isolates to ofloxacin and biofilm formation (r:-0.11; P >0.05). It was found that all sub-MIC concentrations of ofloxacin reduced significantly the biofilm formation on polystyrene and adhesion to HECs in a concentration-dependent manner. Electron microscope images showed that the sub-MIC concentrations of ofloxacin prevent biofilm development to maturation. It can be concluded the negative effect of sub-MIC concentrations of ofloxacin on biofilm production and adhesion to biotic and abiotic surfaces in vitro .
{"title":"Sub-inhibitory doses of Ofloxacin reduce adhesion and biofilm formation of Pseudomonas aeruginosa to biotic and abiotic surfaces","authors":"Lubna Ali Abd Al-mutalib, A. Zgair","doi":"10.29090/psa.2023.03.23.377","DOIUrl":"https://doi.org/10.29090/psa.2023.03.23.377","url":null,"abstract":"Biofilm formation of Pseudomonas aeruginosa ( P. aeruginosa ) and its adherence increases bacterial virulence to cause the infection. The role of treating P. aeruginosa with sub-inhibitory concentrations of ofloxacin in vitro in reducing bacterial adherence to biotic and abiotic surfaces was evaluated. Ten isolates of P. aeruginosa were isolated from urine samples. Biofilm formation on polystyrene microtiter plates and Minimum inhibitory concentrations (MICs) of ofloxacin against all isolates were evaluated. The effect of sub-MICs of ofloxacin (0.5×MIC, 0.25×MIC, 0.125×MIC, and 0.06×MIX) on biofilm formation (to polystyrene) and adhesion to prepared human epithelial cells (HECs) in vitro was evaluated. The MICs of ofloxacin were lower than 64 µg/ml and all isolates produced biofilm. There was no relationship between the susceptibility of bacterial isolates to ofloxacin and biofilm formation (r:-0.11; P >0.05). It was found that all sub-MIC concentrations of ofloxacin reduced significantly the biofilm formation on polystyrene and adhesion to HECs in a concentration-dependent manner. Electron microscope images showed that the sub-MIC concentrations of ofloxacin prevent biofilm development to maturation. It can be concluded the negative effect of sub-MIC concentrations of ofloxacin on biofilm production and adhesion to biotic and abiotic surfaces in vitro .","PeriodicalId":19761,"journal":{"name":"Pharmaceutical Sciences Asia","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77772769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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