Pakistan Veterinary Journal最新文献_第9页

Isolation and in vitro Characterization of Anti-Salmonella Enteritidis Probiotic Potential of Indigenous Lactobacilli from Poultry 鸡源乳酸杆菌抗肠炎沙门氏菌的分离及体外特性研究

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.022

I. Khan

Received: Revised: Accepted: Published online: November 26, 2018 December 31, 2018 January 01, 2019 January 28, 2019 Aim of the present study was to isolate, identify and characterize new indigenous Lactobacillus strains with probiotic potential against Salmonella Enteritidis. From 84 isolated lactobacilli of indigenous poultry origin, 15 isolates were pre-selected for in vitro characterization on the basis of their activity (6.33±0.57-20.33±1.15 mm) against S. Enteritidis by well diffusion assay. All pre-selected isolates had variable tolerance to acidic pH (2, 3 and 4). All isolates also showed growth in MRS broth supplemented with 0.3, 1 and 1.8% bile salts. Isolates had varying degree of auto-aggregation (27.05±0.72 65.87±3.12%) and co-aggregation with S. Enteritidis (6.33±0.11 55.70±1.32%) within 2 hours. Safety profile of lactobacilli indicated that IKP23, IKP 111 and IKP 333 had no acquired antibiotic resistance. IKP 23, IKP 111 and IKP 333 were selected as potential probiotics on the basis of probiotic prerequisites and identified as L. fermentum, L. fermentum and L. salivarius, respectively by sequencing their partial 16S rRNA gene or 16S-23S intergenic spacer region. IKP 23, IKP 111 and IKP 333 inhibited S. Enteritidis (81, 99.3 and 93%, respectively) in co-culture experiments. This study insinuate that IKP 23, IKP 111 and IKP 333 have favourable probiotic potential and may be used for in vivo studies for the development of probiotics against S. Enteritidis. ©2019 PVJ. All rights reserved

接收日期：修订日期：接受日期：在线发布日期：2018年11月26日2018年12月31日2019年1月1日2019年01月28日本研究的目的是分离、鉴定和表征具有对抗肠炎沙门氏菌益生菌潜力的新本土乳杆菌菌株。从84个分离的本地家禽来源的乳酸杆菌中，根据其对肠炎沙门氏菌的活性（6.33±0.57-20.33±1.15mm），通过良好扩散测定，预先选择了15个分离株进行体外鉴定。所有预先选择的分离株对酸性pH（2、3和4）具有可变的耐受性。所有分离株在补充有0.3%、1%和1.8%胆汁盐的MRS肉汤中也显示出生长。分离物在2小时内具有不同程度的自聚集（27.05±0.72 65.87±3.12%）和与肠炎沙门氏菌的共聚集（6.33±0.11 55.70±1.32%）。乳酸杆菌的安全性分析表明，IKP23、IKP111和IKP333没有获得性抗生素耐药性。根据益生菌的先决条件，选择IKP 23、IKP 111和IKP 333作为潜在的益生菌，并通过对其部分16S rRNA基因或16S-23S基因间间隔区进行测序，分别鉴定为发酵乳杆菌、发酵乳杆菌和唾液乳杆菌。在共培养实验中，IKP 23、IKP 111和IKP 333对肠炎沙门氏菌的抑制率分别为81%、99.3%和93%。本研究表明，IKP 23、IKP 111和IKP 333具有良好的益生菌潜力，可用于体内研究开发针对肠炎沙门氏菌的益生菌。©2019 PVJ。保留所有权利

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引用次数: 7

Isolation of Aflatoxigenic Aspergillus flavus from Animal-Feed and Exploration of the Genetic Basis of Aflatoxin Biosynthesis 饲料中黄曲霉的分离及黄曲霉毒素生物合成的遗传基础探讨

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.078

Muhammad Usman

引用次数: 4

The Effect of Routine Therapy Alone and Combined with Hyperbaric Oxygen Treatment on Prognostic Factors in Dogs with Acute Pancreatitis 单纯常规治疗和高压氧联合治疗对犬急性胰腺炎预后因素的影响

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.113

Idehen Ho

Received: Revised: Accepted: Published online: July 18, 2018 August 19, 2019 October 20, 2019 November 05, 2019 The aim of this study was to evaluate the effect of supportive treatment combined with hyperbaric oxygen (HBO) therapy in dogs with acute pancreatitis by prognostic factors. Dogs entering the trial were randomly assigned to receive only regular supportive therapy (control group) or combined hyperbaric oxygen therapy with symptomatic and supportive therapy (HBOT group). The regular supportive measurements consisted of intravenous fluid, analgesia, antibiotics, antiemetics, gastric acid suppression, nutritional management, and so on. If the patient had diabetes mellitus, hyperglycemia was controlled by insulin. The dogs of the HBOT group were conducted in hyperbaric animal chamber (PAHI-A, Pan-America Hyperbarics Inc., R.O.C.) and HBO therapy involved 90 min of 100% oxygen treatment at a pressure of 2.5 ATA, once to twice daily for a total of 3 to 5 sessions. There were significant findings regarding to specific canine pancreatic lipase immunoreactivity (Spec cPL) and C-reactive protein (CRP) in the survivors, but no significant differences were found in the non-survivors. The down trend of sequential CRP concentration was noticed in HBOT group. The level of creatinine may be as an important criterion when the patients with AP were first visit clinic. The prognostic factors such as CRP, Spec cPL had a good response to routine combined with HBOT in survivors group. Likewise, the level of Spec cPL was decreased significantly after treatment no matter routine or routine combined with HBOT in survivors group. HBOT combined with symptomatic and routine treatment might improve systemic inflammation probably in canine acute pancreatitis. In survivors group the creatinine levels was significant lower than that in non-survivors group. Therefore, the levels of creatinine can be as prognostic factor in dogs with acute pancreatitis. ©2019 PVJ. All rights reserved

接收日期：修订日期：接受日期：在线发布日期：2018年7月18日2019年8月19日2019年10月20日2019年11月5日本研究旨在通过预后因素评估支持性治疗结合高压氧（HBO）治疗犬急性胰腺炎的效果。进入试验的狗被随机分配为只接受常规支持性治疗（对照组）或高压氧治疗与症状和支持性治疗相结合（HBOT组）。常规支持性测量包括静脉输液、镇痛、抗生素、止吐药、胃酸抑制、营养管理等。如果患者患有糖尿病，则通过胰岛素控制高血糖。HBOT组的狗在高压动物室（PAHI-A，Pan-America Hyperbarics股份有限公司，R.O.C.）中进行，HBO治疗包括在2.5 ATA的压力下进行90分钟的100%氧气治疗，每天一到两次，共3到5次。存活者的特异性犬胰脂肪酶免疫反应性（Spec-cPL）和C反应蛋白（CRP）有显著发现，但在非存活者中没有发现显著差异。HBOT组连续CRP浓度呈下降趋势。当AP患者首次就诊时，肌酐水平可能是一个重要的标准。生存组CRP、Spec-cPL等预后因素对常规联合HBOT有良好反应。同样，幸存者组的Spec-cPL水平在治疗后显著降低，无论是常规治疗还是常规联合HBOT治疗。HBOT结合症状和常规治疗可能改善犬急性胰腺炎的全身炎症。存活组的肌酸酐水平显著低于非存活组。因此，肌酸酐水平可以作为犬急性胰腺炎的预后因素。©2019 PVJ。保留所有权利

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引用次数: 0

Phenolic Derivatives with Antioxidant and Anti-Inflammatory Activities: An in Silico, in vitro and in vivo Study 具有抗氧化和抗炎活性的酚类衍生物:体外和体内研究

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.052

M. Aqeel

Received: Revised: Accepted: Published online: January 10, 2019 January 29, 2019 January 30, 2019 April 06, 2019 Polyphenols are widely distributed in nature having broad spectrum of biological activities. Most of the biological effects produced by polyphenols are largely attributed to their anti-inflammatory and antioxidant potential. The current research work describes the anti-inflammatory and antioxidant potential of newly synthesized phenolic derivatives (T2, T5, T6, T7 & T8) through in silico, in vitro and in vivo approach. The docking studies were performed against NADPH oxidase (PDB ID 3A1F-) and cyclooxygenase (COX-2 with PDB ID 5KIR Homosapien) using in silico techniques. Amongst phenolic derivatives (T2, T5, T6, T7 &T8) T2 and T6 exhibited maximum binding affinity with target protein NADPH oxidase (6.2 and -6.3 Kcal/mol respectively). However, in case of COX-2 as target protein, all the five newly synthesized phenolic derivatives showed encouraging results. In particular, T2 and T8 with binding affinity values -7.9 Kcal/mol and -8.0 Kcal/mol respectively which are promising than Ibuprofen with binding affinity -6.6 Kcal/mol. Then in vitro antioxidant potential of phenolic derivatives was analyzed through total reducing power, total antioxidant capacity and DPPH (1, 1-diphenyl2picryl-hydrazyl) assay. T2 and T6 showed antioxidant potential with IC50 values of 25.03μg/ml and 37.21 μg/ml respectively as compared to 15.14 μg/ml with respect to ascorbic acid. In vivo anti-inflammatory potential was determined through carrageenan induced paw edema which resulted in T2 with promising percentage edema inhibition (68.64±3.34) as compare to standard piroxicam (83.84±3.87). Amongst all phenolic derivatives T2 has promising results in in silico, in vitro and in vivo studies. ©2019 PVJ. All rights reserved

接收日期：修订日期：接受日期：在线发布日期：2019年1月10日2019年1月份29日2019年01月30日2019年04月06日多酚广泛分布于自然界，具有广泛的生物活性。多酚产生的大多数生物效应在很大程度上归因于其抗炎和抗氧化潜力。目前的研究工作描述了新合成的酚类衍生物（T2、T5、T6、T7和T8）通过硅、体外和体内方法的抗炎和抗氧化潜力。使用计算机技术对NADPH氧化酶（PDB ID 3A1F-）和环氧合酶（COX-2与PDB ID 5KIR Homosapien）进行对接研究。在酚类衍生物（T2、T5、T6、T7和T8）中，T2和T6表现出与靶蛋白NADPH氧化酶的最大结合亲和力（分别为6.2和-6.3 Kcal/mol）。然而，在COX-2作为靶蛋白的情况下，所有五种新合成的酚类衍生物都显示出令人鼓舞的结果。特别地，T2和T8的结合亲和力值分别为-7.9 Kcal/mol和-8.0 Kcal/mmol，这比结合亲和力为-6.6 Kcal/mole的布洛芬更有希望。然后通过总还原力、总抗氧化能力和DPPH（1，1-二苯基-2-二芳基-肼）测定分析了酚类衍生物的体外抗氧化潜力。T2和T6表现出抗氧化潜力，IC50值分别为25.03μg/ml和37.21μg/ml，而抗坏血酸的IC50值为15.14μg/ml。通过卡拉胶诱导的爪水肿测定了体内抗炎潜力，与标准吡罗昔康（83.84±3.87）相比，T2具有良好的水肿抑制百分比（68.64±3.34）。在所有酚类衍生物中，T2在计算机、体外和体内研究中都有良好的结果。©2019 PVJ。保留所有权利

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引用次数: 0

Assessment of Ginger Extract and Ginger Nanoparticles Protective Activity against Acetaminophen-Induced Hepatotoxicity and Nephrotoxicity in Rats 生姜提取物和生姜纳米粒子对对对乙酰氨基酚肝毒性和肾毒性的保护作用

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.060

Alaa F. Bakr

Received: Revised: Accepted: Published online: November 28, 2018 March 30, 2019 April 06, 2019 April 23, 2019 Acetaminophen is widely used analgesics all over the world. However, hepatotoxicity and nephrotoxicity are the most features of acetaminophen overdose. Ginger is a medicinal plant that has anti-tumorigenic, anti-apoptotic, anti-oxidant activities. Recently, ginger nanoparticles have been investigated in liver protection against alcohol-induced liver damage and in treatment of bowel colitis. Our experiment was aimed to judge the protective activity of ginger nanoparticles relative to ginger extract at dose of 120 mg/kg against acetaminophen toxicity at dose of 375 mg/kg (1/10 LD50) daily for three months in male rats from the histological and biochemical aspects. Serum Alanine aminotransferase and aspartate aminotransferase were determined. In addition, urea and uric acid levels were evaluated in serum. Moreover, oxidative stress was evaluated by determining malondialdehyde content and catalase enzyme activity. Meanwhile, histopathological changes in liver and kidney tissues were observed. The present study indicates that liver and kidney biochemical markers, oxidative stress and histopathological structure are improved in rat pretreated with ginger extract. However, rats treated with GNPs were more protective relative to ginger extract pre-treated rat. ©2019 PVJ. All rights reserved

收稿日期:修订日期:接受日期:在线发布日期:2018年11月28日2019年3月30日2019年4月06日2019年4月23日对乙酰氨基酚是世界范围内广泛使用的镇痛药。然而，肝毒性和肾毒性是对乙酰氨基酚过量的最大特征。生姜是一种具有抗肿瘤、抗细胞凋亡、抗氧化活性的药用植物。最近，生姜纳米颗粒在肝脏保护中对酒精引起的肝损伤和治疗肠结肠炎进行了研究。本实验旨在从组织学和生化方面判断生姜纳米颗粒相对于生姜提取物在120 mg/kg剂量下对375 mg/kg (1/10 LD50)剂量连续3个月对雄性大鼠的对乙酰氨基酚毒性的保护作用。测定血清丙氨酸转氨酶和天冬氨酸转氨酶。此外，测定血清尿素和尿酸水平。此外，通过测定丙二醛含量和过氧化氢酶活性来评估氧化应激。同时观察肝、肾组织病理变化。本研究表明，生姜提取物对大鼠肝肾生化指标、氧化应激及组织病理结构均有改善作用。然而，GNPs处理的大鼠相对于生姜提取物预处理的大鼠更具保护作用。©2019 PVJ。版权所有

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引用次数: 20

Characterization of Salmonella Enterica Isolated from Poultry Hatcheries and Commercial Broiler Chickens 家禽孵化场和商品肉鸡肠炎沙门氏菌的分离鉴定

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.033

A. Shehata

Received: Revised: Accepted: Published online: October 04, 2018 November 26, 2018 December 20, 2018 March 07, 2019 Salmonella, is a serious pathogen causing disastrous losses in the poultry production and dangerous human infections. This study was aimed to identify Salmonella serotypes and to detect the prominent virulence genes and antimicrobial resistance of the obtained serotypes from samples collected from dead chicken embryos, dead duck embryos and commercial broilers. The pathogenicity of some serotypes as (Salmonella Pullorum, Salmonella Enteritidis, and Salmonella Typhimurium) was investigated in one-day-old commercial layer chicks. A total of 180 cases were collected, after isolation 18 Salmonella isolates were reported to be as follows 7/100(7%), 5/40(12.5%) and 6/40(15%) in broilers, dead chickens, and dead duck embryos, respectively. The most prevalent serotypes were S. Enteritidis, S. Typhimurium, S. Pullorum, S. Vejle, S. Amsterdam, S. Infantis, S. Petersburg, and S. Atakpame. The distribution patterns of virulence genes iroN, cdtB, spaN, invA, and orgA, expressed as follows; 17/18(94.4%), 15/18(83.3%), 14/18(77.7%), 13/18(72.2%), and 12/18(66.7%) of serotypes, respectively. Added to that, the sipV, IpfC, sopB, prgH, and sitC virulence genes were amplified in 7/18(38.8%), 7/18(38.8%), 7/18(38.8%), 5/18(27.7%) and 3/18(16.6%) of isolates respectively. Most of isolates 16/18 (88.9%) expressed multiple antibiotic resistances (MAR) indices ranged from ≥0.3 ≤1. Based on the pathogenicity testing, S. Pullorum was the most pathogenic due to the clear signs and a mortality rate of 35%. Hence, the genotypic characterization and continuous monitoring of antimicrobial resistance of Salmonella from poultry are of public health concern to implement effective control strategies against this notorious pathogen. ©2018 PVJ. All rights reserved

接收日期：修订日期：接受日期：在线发布日期：2018年10月4日2018年11月26日2018年12月20日2019年3月7日沙门氏菌是一种严重的病原体，在家禽生产中造成灾难性损失和危险的人类感染。本研究旨在鉴定沙门氏菌血清型，并检测从死鸡胚胎、死鸭胚胎和商品肉鸡中采集的样品中获得的血清型的显著毒力基因和抗微生物耐药性。在一日龄商品蛋鸡中研究了一些血清型（白氏沙门氏菌、肠炎沙门氏菌和鼠伤寒沙门氏菌）的致病性。共收集了180例病例，分离后，肉鸡、死鸡和死鸭胚胎中的沙门氏菌分离株分别为7/100（7%）、5/40（12.5%）和6/40（15%）。最流行的血清型是肠炎沙门氏菌、鼠伤寒沙门氏菌（S.Typhimurium）、普伦沙门氏菌，维勒沙门氏菌和阿姆斯特丹沙门氏菌。毒力基因iroN、cdtB、spaN、invA和orgA的分布模式如下所示；血清型分别为17/18（94.4%）、15/18（83.3%）、14/18（77.7%）、13/18（72.2%）和12/18（66.7%）。此外，sipV、IpfC、sopB、prgH和sitC毒力基因分别在7/18（38.8%）、7/18（38.1%）、5/18（27.7%）和3/18（16.6%）的分离株中扩增。大多数分离株16/18（88.9%）表达的多重耐药（MAR）指数在≥0.3≤1之间。根据致病性测试，Pullorum是最具致病性的，因为其症状明显，死亡率为35%。因此，对家禽中沙门氏菌的基因型特征和耐药性的持续监测是公共卫生关注的问题，以实施针对这种臭名昭著的病原体的有效控制策略。©2018 PVJ。保留所有权利

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引用次数: 12

Spectrophotometric Calibration and Comparison of Different Semen Evaluation Methods in Nili-Ravi Buffalo Bulls Nili Ravi水牛不同精液评价方法的分光光度法校准及比较

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.073

Saif-ur-Rehman, Laiba Shafique, M. Yousuf, Liu Qingyou, J. Ahmed, H. Riaz

Received: Revised: Accepted: Published online: November 28, 2018 March 28, 2019 April 01, 2019 May 20, 2019 This study was designed with the aim to find suitable spectrophotometric wavelength for accurate measurement of sperm concentration in Nili Ravi buffalo and to compare different quantitative methods (visual, hemocytometric and spectrophotometric) for the assessment of sperm concentration. A total of 78 fresh semen ejaculate were collected from breeding buffalo bulls, diluted with required concentrations and relationship between sperm number and absorbance was determined at 350, 450, 546 and 650 nm wavelengths by using spectrophotometer. The spectrometric results showed that λmax was obtained at 350 nm. The derived equation between sperm concentration counted by haemocytometer and absorbance at 350 nm was Y = 0.1135x + 0.0002 (R = 0.9924), at 450 nm, Y = 0.1011x-0.0436 (R = 0.9756), at 546nm, Y = 0.0825x-0.0101 (R = 0.9603) and at 650 nm, Y = 0.0774x-0.0235 (R = 0.9602). These results were later compared with relative sperm numbers at different dilutions. The results showed that 350nm wavelength appeared suitable for estimation of sperm concentration compared to others. Additionally, it was found that both photometer(P<0.01) and haemocytometer (P<0.01) significantly counted more sperm cells than visual assessment as used regularly on different SPUs. Moreover, haemocytometer counted significantly (834×10Vs 678×10, P=0.005) more sperm cell than Photometer. Finally, regression analysis between haemocytometer and Photometer showed significant slope of regression and regression coefficient was 0.771 which warns that photometer must be calibrated before proper employment for routine measurement of sperm concentration. ©2019 PVJ. All rights reserved

本研究旨在寻找合适的分光光度波长，以准确测量尼利拉维水牛的精子浓度，并比较不同的定量方法(目测法、血细胞法和分光光度法)对精子浓度的评估。采用分光光度计，在350、450、546和650 nm波长下，采集了78只纯种水牛的新鲜射精，用所需浓度稀释，测定了精子数量与吸光度的关系。光谱分析结果表明，在350 nm处获得λmax。在350 nm处，Y = 0.1135x + 0.0002 (R = 0.9924)，在450 nm处，Y = 0.1011x-0.0436 (R = 0.9756)，在546nm处，Y = 0.0825x-0.0101 (R = 0.9603)，在650 nm处，Y = 0.0774x-0.0235 (R = 0.9602)。这些结果随后与不同稀释度下的相对精子数量进行了比较。结果表明，与其他波长相比，350nm波长更适合用于估计精子浓度。此外，光度计(P<0.01)和血细胞计(P<0.01)在不同spu上使用的精子计数明显多于目测。此外，血细胞计的精子计数明显多于光度计(834×10Vs 678×10, P=0.005)。最后，血球计与光度计的回归分析显示回归斜率显著，回归系数为0.771，提示光度计在正常用于精子浓度的常规测量之前必须进行校准。©2019 PVJ。版权所有

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引用次数: 13

Identification of a Novel Genotype of Streptococcus equi Subspecies equi in a Donkey Suffering from Strangles 一种新型马链球菌基因型的鉴定

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.087

Yanlin Liu

Received: Revised: Accepted: Published online: June 11, 2019 July 09, 2019 July 10, 2019 July 30, 2019 Strangles is a contagious bacterial disease of equines and is prevalent worldwide. Host-restricted Streptococcus equi subspecies equi is the etiology. In April 2019, strangles was diagnosed in a donkey in the quarantine area of an intensive donkey farm. The infection was confirmed by clinical signs, bacterial isolation, histochemistry, PCR and sequencing. Based on the M-protein typing method, analysis of SeM sequencing data indicated that the isolated S. equi strain CN190402 belongs to a novel genotype SeM-138. The present study increases knowledge of the molecular epidemiology of S. equi and will lead to a greater understanding of the diversity of this pathogen. Meanwhile, it emphasized the necessity of quarantine areas and the importance of biosecurity for intensive donkey farms to prevent future outbreaks. ©2019 PVJ. All rights reserved

接收日期：修订日期：接受日期：在线发布日期：2019年6月11日2019年7月9日2019年07月10日2019年06月30日搁浅是一种马的传染性细菌性疾病，在全球范围内流行。宿主限制性马链球菌是马亚种的病因。2019年4月，在一个集约化驴场的隔离区，一头驴被诊断为勒死。通过临床症状、细菌分离、组织化学、聚合酶链式反应和测序证实感染。基于M蛋白分型方法，对测序数据的分析表明，分离的马S.equi菌株CN190402属于一个新的基因型SeM-138。本研究增加了对马的分子流行病学的了解，并将有助于更好地了解这种病原体的多样性。同时，它强调了隔离区的必要性和生物安全对集约化驴场的重要性，以防止未来疫情的爆发。©2019 PVJ。保留所有权利

引用次数: 3

Effect of Dietary Vitamin E and Selenium Supplementation on Physiological Responses and Reproductive Performance in Holstein Friesian Bulls during Humid Hot Summer 饲粮中添加维生素E和硒对湿热夏季荷斯坦弗里阉牛生理反应和繁殖性能的影响

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.053

Mahboob Ahmed Butt

Received: Revised: Accepted: Published online: February 19, 2019 March 15, 2019 March 20, 2019 April 06, 2019 The objective of this study was to assess the effect of dietary vitamin E (Vit. E) and Selenium (Se) supplementation on physiological and reproductive performance of bulls during humid hot summer in Pakistan. Eighteen Holstein Friesian bulls balanced by age and weight were divided into three treatment groups: 1) CTL, basal diet without Se and Vit. E supplementation; 2) SeS, basal diet supplemented with 3 g Selemax® (Biorigin, USA); 3) ESeS, basal diet with 3 g Selemax® and Vit. E @ 6000 IU/day). Basal diet consisted of 50% green fodder, 25% wheat straw and 25% concentrate on dry matter basis. Diets were offered for 13 weeks from July 2016 to October 2016 having temperature-humidity index ranging from 83 to 87. Data obtained were subjected to repeated measures ANOVA. Supplementation of Vit. E and Se did not affect dry matter intake, water intake, respiration rate, rectal temperature, and pulse rate. The ESeS treatment had higher sperm motility% compared to CTL and SeS (P<0.05). The amplitude of lateral head displacement (ALH) was higher in SeS and ESeS compared to CTL (P<0.05). Straightness was higher in SeS group compared to CTL and ESeS. However, Vit. E and Se had no effect on other semen traits including semen volume, concentration, progressive motility, DNA integrity, plasma membrane integrity, and live to dead ratio. Blood testosterone concentration was significantly higher in SeS and ESeS groups compared to CTL. Blood glucose and blood urea nitrogen were similar among the treatments. Current results indicated that supplementation of Vit. E and Se improved only sperm motility, ALH, straightness and testosterone level during humid hot summer in Holstein Friesian bulls. ©2019 PVJ. All rights reserved

接收日期：修订日期：接受日期：在线发布日期：2019年2月19日2019年3月15日2019年4月20日本研究的目的是评估在巴基斯坦湿热的夏季，补充膳食维生素E（Vit.E）和硒（Se）对公牛生理和繁殖性能的影响。18头荷斯坦-弗里斯公牛按年龄和体重平衡，分为三组：1）CTL、不含Se和Vit的基础日粮。E补充；2） SeS，基础日粮补充3 g Selemax®（Biorigin，美国）；3） ESeS，含3 g Selemax®和Vit的基础饮食。E@6000 IU/天）。基础日粮由50%的绿色饲料、25%的小麦秸秆和25%的干物质浓缩物组成。从2016年7月到2016年10月，提供为期13周的饮食，温湿度指数在83到87之间。对获得的数据进行重复测量ANOVA。补充维生素。E和Se不影响干物质摄入量、水分摄入量、呼吸频率、直肠温度和脉搏率。与CTL和SeS相比，ESeS处理的精子活力%更高（P＜0.05）。SeS和ESeS组的头部侧向位移幅度（ALH）高于CTL（P＜0.01）。然而，Vit。E和Se对其他精液性状没有影响，包括精液体积、浓度、进行性运动、DNA完整性、质膜完整性和活死亡率。SeS和ESeS组的血睾酮浓度明显高于CTL。血糖和血尿素氮在不同处理之间是相似的。目前的结果表明，补充维生素。在湿热的夏季，E和Se只改善了荷斯坦-弗里斯公牛的精子活力、ALH、平直度和睾酮水平。©2019 PVJ。保留所有权利

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引用次数: 7

Genetic Characterization of Foot and Mouth Disease Virus (FMD) Serotypes in Egypt (2016-2017) and Identification of a New Lineage of Serotype O Topotype EA-3 2016-2017年埃及口蹄疫病毒(FMD)血清型的遗传特征及O型拓扑型EA-3新谱系的鉴定

IF 2.3 3区农林科学 Q1 Veterinary

Pakistan Veterinary Journal

Pub Date : 2019-10-01 DOI: 10.29261/pakvetj/2019.061

M. Soltan, Abdel-Hamid I. Bazid, M. Fawzy, M. Wasfy, S. M. Soliman, M. Shahein, M. Elsayed

Received: Revised: Accepted: Published online: November 28, 2018 March 01, 2019 March 08, 2019 April 23, 2019 From December 2016 to April 2017, many farmers and veterinarians complained about FMD outbreaks in various Egyptian governorates. Therefore; the present study aimed to characterize the circulating FMD viruses during this period. Clinical data and vaccination histories were collected from nine affected farms and 27 individual cases owned by small farmers in 10 Egyptian governorates. A total of 59 vesicular epithelia were gathered and tested by real-time (rt) RT-PCR to detect and quantify the virus. Furthermore, the positive samples with sufficient genomic loads were analyzed by the less sensitive conventional RT-PCR to amplify the full length of the VP1 region; amplicons were sequenced and phylogenetic trees were constructed. Of 59 samples analyzed, 49 (83.1%) were positive by rt RT-PCR, but only 22/39 produced amplicons by conventional RT-PCR. About 19 amplicons were suitable for sequencing and showed serotypes O, A, and SAT2 in 15, 1 and 3 samples, respectively. Phylogenetic analyses clustered the characterized strains in serotypes O, topotypes EA-3; serotype A, African topotype of genotype IV; and serotype SAT2, topotype VII, respectively. Interestingly, two lineages of serotype O topotype EA-3 strains were distinguished and referred to as Ism-16 and Alx-17. Ism-16 lineage clustered with the previously characterized viruses in Egypt in 2016, but the Alx-17 lineage clustered in a separate clade and is hereby reported for the first time. Further studies are required to evaluate the cross-protection between the vaccine and heterologous circulating strains in Egypt, especially for serotype O. ©2019 PVJ. All rights reserved

收到：修订：接受：在线发布：2018年11月28日2019年3月1日2019年03月08日2019年04月23日从2016年12月到2017年4月，许多农民和兽医抱怨埃及各省爆发了口蹄疫。因此本研究旨在对这一时期流行的口蹄疫病毒进行表征。临床数据和疫苗接种史是从埃及10个省的9个受影响农场和27个小农户拥有的个体病例中收集的。共收集59个水疱上皮细胞，并通过实时（rt）rt-PCR检测和定量病毒。此外，通过不太敏感的常规RT-PCR分析具有足够基因组负载的阳性样品，以扩增VP1区域的全长；对扩增子进行测序并构建系统发育树。在分析的59个样本中，49个（83.1%）通过rt rt-PCR呈阳性，但只有22/39通过常规rt-PCR产生扩增子。大约19个扩增子适合测序，分别在15个、1个和3个样本中显示血清型O、A和SAT2。系统发育分析将特征菌株聚类为血清型O、拓扑型EA-3；血清型A，基因型IV的非洲拓扑型；和血清型SAT2，拓扑型VII。有趣的是，区分了血清型O拓扑型EA-3菌株的两个谱系，称为Ism-16和Alx-17。2016年，在埃及，Ism-16谱系与先前表征的病毒聚集在一起，但Alx-17谱系聚集在一个单独的分支中，这是首次报道。需要进一步的研究来评估疫苗和埃及异源循环菌株之间的交叉保护，特别是血清型O。©2019 PVJ。保留所有权利

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引用次数: 10