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Basic Analysis of Glycolysis in Cardiac Tissue in Broiler Chickens Presenting with Ascites Syndrome 肉鸡腹水综合征心脏组织糖酵解的基本分析
IF 2.3 3区 农林科学 Q1 Veterinary Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.060
Yaming Ge
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引用次数: 3
Characterization of Infectious Laryngotracheitis Virus Isolates from Turkey by Molecular and Sequence Analysis 土耳其传染性喉气管炎病毒分离株的分子和序列分析
IF 2.3 3区 农林科学 Q1 Veterinary Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.051
K. Can-Sahna
Received: Revised: Accepted: Published online: February 22, 2020 April 27, 2020 May 12, 2020 June 08, 2020 Infectious laryngotracheitis (ILT) is an economically important respiratory disease affecting the poultry industry worldwide. The aim of this study was to characterize the Turkish ILT virus (ILTV) isolates by sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). ILTV isolates obtained from laying hens in Turkey in 2003 and 2018 were used in this study. The isolates were analyzed for ICP4, gG, gE and TK gene regions by PCR. The amplification products were used in RFLP analysis to determine the differences among the isolates. Sequence analyses of TK and ICP4 regions were carried out and a phylogenetic tree was formed by using the Maximum Likelihood method. Nucleotide identity values were then calculated among five isolates and other strains/isolates in Genbank. In addition, about 200 amino acid sequences of the start and end regions of the ICP4 gene were compared to other strains in Genbank. PCR-RFLP analysis indicated that Turkish ILTV isolates were low-virulent. In general, the nucleotide sequence similarities of the TK and ICP4 gene regions among Turkish isolates and others was more than 95% (lower in some Egyptian and Bangladeshi strains, 41 and 45% respectively); in the amino acid sequence, it was close to 100%. As a result, PCR-RFLP results were similar in many gene regions. However, evolutionary analysis of ICP4 and TK gene regions did not yield reliable results based on geographic distribution or pathogenicity levels. For this purpose, different methods, such as Bayesian analysis or the involvement of samples from different gene regions can yield more reliable results, just like whole-genome sequences. ©2020 PVJ. All rights reserved
感染性喉气管炎(ILT)是影响全球家禽业的一种经济上重要的呼吸道疾病。本研究的目的是通过测序和聚合酶链反应-限制性片段长度多态性(PCR-RFLP)来表征土耳其ILT病毒(ILTV)分离株。本研究使用了2003年和2018年从土耳其蛋鸡中获得的ILTV分离株。采用PCR方法对分离株的ICP4、gG、gE和TK基因区进行分析。利用扩增产物进行RFLP分析,确定菌株间的差异。对TK和ICP4区进行序列分析,采用极大似然法构建系统发育树。然后计算5株分离株和Genbank中其他菌株/分离株的核苷酸同一性值。此外,还将ICP4基因起始和结束区域的约200个氨基酸序列与Genbank中的其他菌株进行了比较。PCR-RFLP分析表明,土耳其ILTV分离株毒性较低。总体而言,土耳其菌株与其他国家菌株的TK和ICP4基因区核苷酸序列相似性均在95%以上(埃及和孟加拉国部分菌株分别为41%和45%);在氨基酸序列上,接近100%。因此,PCR-RFLP结果在许多基因区域是相似的。然而,ICP4和TK基因区域的进化分析并没有基于地理分布或致病性水平得出可靠的结果。为此,不同的方法,如贝叶斯分析或涉及来自不同基因区域的样本,可以产生更可靠的结果,就像全基因组测序一样。©2020 PVJ。版权所有
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引用次数: 2
Evaluation of Passive Immunity Transfer against G6P[1] Rotavirus in Holstein Calves by ELISA ELISA法评价荷斯坦犊牛对G6P[1]轮状病毒的被动免疫转移
IF 2.3 3区 农林科学 Q1 Veterinary Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.031
T. G. Rocha
The main strategy to prevent bovine rotavirus group A (RVA) diarrhea in calves is to vaccinate late-term dams aiming to enhance passive immunity transfer of specific immunoglobulins against the virus This study aimed to evaluate influence of parity in titers of immunoglobulin G (IgG), IgG1 and IgM in serum and colostrum of vaccinated or unvaccinated Holstein cows and in serum of its calves, associated with monitoring for RVA diarrhea in calves Cows and its calves were allotted into groups according to parity and vaccination (primiparous/multiparous;vaccinated/unvaccinated) and serum and colostrum samples of cows were taken as well as serum and fecal samples of its calves Parturition influenced colostral titers of IgG and IgG1, which were higher in multiparous cows, whilst IgM titers were influenced by vaccination, being higher in colostrum of vaccinated dams Lowest serum titers of IgG and IgG1 were found in calves born to unvaccinated primiparous dams Eleven calves presented RVA diarrhea, and genotypes G6P[11] and G6P[5] were found in the vaccinated and unvaccinated herds, respectively Vaccination of dams prolongs humoral immunity in calves and enhances colostrum quality and should be a primary concern in primiparous cows (C) 2020 PVJ All rights reserved
预防犊牛轮状病毒A组(RVA)腹泻的主要策略是为犊牛接种疫苗,以增强犊牛对该病毒的特异性免疫球蛋白的被动免疫转移。本研究旨在评估胎次对接种或未接种的荷斯坦奶牛血清和初乳以及犊牛血清中免疫球蛋白G (IgG)、IgG1和IgM滴度的影响。根据胎次和疫苗接种情况(初产/多产;接种疫苗/未接种疫苗)将奶牛及其犊牛分为不同的组,并采集奶牛的血清和初乳样本以及犊牛的血清和粪便样本。分娩影响初产奶牛的IgG和IgG1滴度,多产奶牛的IgG和IgG1滴度较高,而IgM滴度受疫苗接种的影响。未接种疫苗的犊牛所生的犊牛血清IgG和IgG1滴度最低。11头犊牛出现RVA腹泻,分别在接种疫苗和未接种疫苗的犊牛中发现G6P[11]和G6P[5]基因型,接种疫苗可延长犊牛的体液免疫,提高初乳质量,应成为犊牛的首要关注问题(C) 2020 PVJ
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引用次数: 1
Molecular Epidemiology and Characteristics of Streptococcus agalactiae Isolated from Bovine Mastitis in Large Dairy Herds of China 中国大型奶牛乳腺炎无乳链球菌分离株的分子流行病学及特征
IF 2.3 3区 农林科学 Q1 Veterinary Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.025
Yang XueYun, Wang DengFeng, Li Jianjun, Meng XiaoXiao, Wei YuRong, Tuerxun Gunuer, Jin Yinghong, Xue Jing, Tariq Ali, Han Bo, Jia Bin
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引用次数: 3
ESBL-Producing Escherichia coli from Bovine Mastitis Induced Apoptosis of Bovine Mammary Epithelial Cells Via Alteration of ROS/MMP/bax/bcl-2 Signaling Pathway 产esbl的牛乳腺炎大肠杆菌通过改变ROS/MMP/bax/bcl-2信号通路诱导牛乳腺上皮细胞凋亡
IF 2.3 3区 农林科学 Q1 Veterinary Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.038
Yuxiang Shi
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引用次数: 1
Single Nucleotide Polymorphisms in the Promoter of CD4 Gene are Associated with Production and Mastitis Traits in Dairy Cattle 奶牛CD4基因启动子单核苷酸多态性与奶牛产量和乳腺炎性状相关
IF 2.3 3区 农林科学 Q1 Veterinary Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.057
Arsalan Rasheed
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引用次数: 4
Detection of Genetic Variations in Pattern Recognition Receptors (PRRs) Gene of Tharparkar Cattle Breed 塔帕卡牛模式识别受体(PRRs)基因遗传变异的检测
IF 2.3 3区 农林科学 Q1 Veterinary Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.032
L. Iqbal
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引用次数: 1
Occurrence and Molecular Characterization of Shiga Toxin-Producing Escherichia coli Isolates Recovered from Cattle and Goat Meat Obtained from Retail Meat Shops in Rawalpindi and Islamabad, Pakistan 巴基斯坦拉瓦尔品第和伊斯兰堡零售肉店牛肉和羊肉中产志贺毒素大肠杆菌分离株的发生及分子特征
IF 2.3 3区 农林科学 Q1 Veterinary Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.045
H. Irshad
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引用次数: 2
Prevalence of Antibiotic-Resistant and Extended-Spectrum Beta-Lactamase-Producing Escherichia coli in Chicken Meat from Eastern Turkey 产广谱β -内酰胺酶的耐药大肠杆菌在东土耳其鸡肉中的流行
IF 2.3 3区 农林科学 Q1 Veterinary Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.047
A. Baran
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引用次数: 11
Developing a Novel, Efficient and Cost Effective Tissue Injury Model (in-vitro) on Equine Tendon Fibroblasts 建立一种新颖、高效、经济的马肌腱成纤维细胞体外组织损伤模型
IF 2.3 3区 农林科学 Q1 Veterinary Pub Date : 2020-01-01 DOI: 10.29261/pakvetj/2020.058
Somia Shehzadi
{"title":"Developing a Novel, Efficient and Cost Effective Tissue Injury Model (in-vitro) on Equine Tendon Fibroblasts","authors":"Somia Shehzadi","doi":"10.29261/pakvetj/2020.058","DOIUrl":"https://doi.org/10.29261/pakvetj/2020.058","url":null,"abstract":"","PeriodicalId":19845,"journal":{"name":"Pakistan Veterinary Journal","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69870030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Pakistan Veterinary Journal
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