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Circularly polarized lasing from chiral metal-organic frameworks 来自手性金属有机框架的圆偏振激光
IF 7.6 1区 物理与天体物理 Q1 OPTICS Pub Date : 2024-05-29 DOI: 10.1364/prj.520965
Dexiang Zhu, Zhouyuanhang Wang, Xiangyu Xu, Wenyu Du, Wei Huang, Yan Kuai, Benli Yu, Jianzhong Zheng, Zhijia Hu, Siqi Li
Circularly polarized lasers play a pivotal role in classical optics, nanophotonics, and quantum optical information processing, while their fabrication remains complex. This article begins with examining the interactions between stimulated emission and chiral matter, outlining a simple strategy for producing circularly polarized lasing from chiral metal-organic frameworks (MOFs), such as the zeolitic imidazolate framework (ZIF), embedded with achiral laser dyes (L/D-ZIFdyes). It is found that the stimulated emission threshold and intensity are influenced by the interplay between the chiral polarization of the pump light and the inherent chirality of the MOF nanoparticles. We further present the design of a chiral vertical-cavity surface-emitting laser (VCSEL), comprising an L/D-ZIFdyes solid-state film sandwiched between a high-reflectivity distributed Bragg reflector (DBR) mirror and a silver film. The cavity-based lasing exhibits higher asymmetry between emitting left-handed and right-handed polarized light compared to chiral spontaneous emission (SE) and amplified spontaneous emission (ASE), with an asymmetry value g lum of approximately ±0.31. This value is nearly four-fold greater than that of SE and twice that of ASE. Our findings reveal a new approach to amplify chiral signals, promoting the comprehension and application of chiral–matter interactions, and offering a simple yet effective strategy to fabricate chiral lasers.
圆偏振激光器在经典光学、纳米光子学和量子光学信息处理中发挥着举足轻重的作用,但其制造过程仍然十分复杂。本文首先探讨了受激发射与手性物质之间的相互作用,概述了利用手性金属有机框架(MOF)(如沸石咪唑框架(ZIF))产生圆偏振激光的简单策略,并嵌入了非手性激光染料(L/D-ZIF⊃dyes)。研究发现,受激发射阈值和强度受泵浦光的手性偏振和 MOF 纳米粒子固有手性之间相互作用的影响。我们进一步介绍了手性垂直腔面发射激光器(VCSEL)的设计,它由夹在高反射率分布式布拉格反射镜(DBR)和银膜之间的 L/D-ZIF⊃dyes 固体薄膜组成。与手性自发辐射(SE)和放大自发辐射(ASE)相比,基于空腔的激光在发射左手偏振光和右手偏振光之间表现出更高的不对称性,不对称性值 g lum 约为±0.31。这一数值几乎是 SE 的四倍,是 ASE 的两倍。我们的发现揭示了一种放大手性信号的新方法,促进了对手性物质相互作用的理解和应用,并为制造手性激光器提供了一种简单而有效的策略。
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引用次数: 0
Electric tuning of plasmonic resonances in ultrathin gold nanoribbon arrays 超薄金纳米带阵列中等离子共振的电调谐
IF 7.6 1区 物理与天体物理 Q1 OPTICS Pub Date : 2024-05-29 DOI: 10.1364/prj.522533
Zhenxin Wang, Alexey V. Krasavin, Chenxinyu Pan, Junsheng Zheng, Zhiyong Li, Xin Guo, Anatoly V. Zayats, Limin Tong, Pan Wang
Ultrathin plasmonic nanostructures offer an unparalleled opportunity for the study of light–matter interactions at the nanoscale and realization of compact nanophotonic devices. In this study, we introduce an ultrathin gold nanoribbon array and demonstrate an electric approach to actively tuning its plasmonic resonance, which leveraging the extreme light confinement capability in the ultrathin plasmonic nanostructure and a robust nanoscale electro-optical effect in indium tin oxide. Optimizing the design (to a total thickness as small as 12 nm for a 2-nm-thick gold nanoribbon array), we numerically demonstrate a spectral shift in the plasmonic resonance up to 36 nm along with an approximately 16% change in the transmission at a gate voltage below 1.7 V at the wavelength of 1.47 μm. This work presents progress towards electric tuning of plasmonic resonances in ultrathin metallic nanostructures for various applications including surface-enhanced spectroscopy, spontaneous emission enhancement, and optical modulation.
超薄等离子纳米结构为研究纳米尺度的光物质相互作用和实现紧凑型纳米光子器件提供了无与伦比的机会。在这项研究中,我们介绍了一种超薄金纳米带阵列,并展示了一种主动调谐其等离子共振的电学方法,这种方法利用了超薄等离子纳米结构中的极强光束约束能力和氧化铟锡中稳健的纳米级电光效应。通过优化设计(将 2 纳米厚的金纳米带阵列的总厚度减小到 12 纳米),我们从数值上证明,在波长为 1.47 μm 的情况下,栅极电压低于 1.7 V 时,等离子共振的光谱偏移可达 36 纳米,透射率变化约为 16%。这项工作展示了在超薄金属纳米结构中对等离子共振进行电调谐以实现各种应用(包括表面增强光谱学、自发辐射增强和光学调制)方面取得的进展。
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引用次数: 0
Integrated photonic fractional convolution accelerator 集成光子分数卷积加速器
IF 7.6 1区 物理与天体物理 Q1 OPTICS Pub Date : 2024-05-23 DOI: 10.1364/prj.517491
Kevin Zelaya, Mohammed-Ali Miri
An integrated photonic circuit architecture to perform a modified-convolution operation based on the discrete fractional Fourier transform (DFrFT) is introduced. This is accomplished by utilizing two nonuniformly coupled waveguide lattices with equally spaced eigenmode spectra, the lengths of which are chosen so that the DFrFT and its inverse operations are achieved. A programmable modulator array is interlaced so that the required fractional convolution operation is performed. Numerical simulations demonstrate that the proposed architecture can effectively perform smoothing and edge detection tasks even for noisy input signals, which is further verified by electromagnetic wave simulations. Notably, mild lattice defects do not jeopardize the architecture performance, showing its resilience to manufacturing errors.
本文介绍了一种基于离散分数傅里叶变换(DFrFT)执行修正卷积操作的集成光子电路结构。这是通过利用两个非均匀耦合波导晶格来实现的,这两个晶格具有等距的特征模式光谱,其长度的选择是为了实现 DFrFT 及其逆运算。可编程调制器阵列交错排列,以便执行所需的分数卷积操作。数值模拟证明,即使输入信号有噪声,所提出的架构也能有效执行平滑和边缘检测任务,电磁波模拟进一步验证了这一点。值得注意的是,轻微的晶格缺陷并不会影响该架构的性能,这显示了它对制造误差的适应能力。
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引用次数: 0
Symmetric silicon microring resonator optical crossbar array for accelerated inference and training in deep learning 用于深度学习中加速推理和训练的对称硅微栅谐振器光交叉棒阵列
IF 7.6 1区 物理与天体物理 Q1 OPTICS Pub Date : 2024-05-23 DOI: 10.1364/prj.520518
Rui Tang, Shuhei Ohno, Ken Tanizawa, Kazuhiro Ikeda, Makoto Okano, Kasidit Toprasertpong, Shinichi Takagi, Mitsuru Takenaka
Photonic integrated circuits are emerging as a promising platform for accelerating matrix multiplications in deep learning, leveraging the inherent parallel nature of light. Although various schemes have been proposed and demonstrated to realize such photonic matrix accelerators, the in situ training of artificial neural networks using photonic accelerators remains challenging due to the difficulty of direct on-chip backpropagation on a photonic chip. In this work, we propose a silicon microring resonator (MRR) optical crossbar array with a symmetric structure that allows for simple on-chip backpropagation, potentially enabling the acceleration of both the inference and training phases of deep learning. We demonstrate a 4×4 circuit on a Si-on-insulator platform and use it to perform inference tasks of a simple neural network for classifying iris flowers, achieving a classification accuracy of 93.3%. Subsequently, we train the neural network using simulated on-chip backpropagation and achieve an accuracy of 91.1% in the same inference task after training. Furthermore, we simulate a convolutional neural network for handwritten digit recognition, using a 9×9 MRR crossbar array to perform the convolution operations. This work contributes to the realization of compact and energy-efficient photonic accelerators for deep learning.
利用光固有的并行特性,光子集成电路正在成为深度学习中加速矩阵乘法的一个前景广阔的平台。虽然已经提出并演示了各种实现这种光子矩阵加速器的方案,但由于在光子芯片上直接进行片上反向传播存在困难,因此使用光子加速器对人工神经网络进行原位训练仍然具有挑战性。在这项工作中,我们提出了一种具有对称结构的硅微扰谐振器(MRR)光交叉棒阵列,它可以实现简单的片上反向传播,从而有可能加速深度学习的推理和训练阶段。我们在硅-绝缘体平台上演示了 4×4 电路,并用它来执行简单神经网络的推理任务,对虹膜花进行分类,分类准确率达到 93.3%。随后,我们使用模拟片上反向传播训练神经网络,训练后在相同推理任务中的准确率达到 91.1%。此外,我们还模拟了一个用于手写数字识别的卷积神经网络,使用 9×9 MRR 交叉条阵执行卷积运算。这项工作有助于实现用于深度学习的紧凑型高能效光子加速器。
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引用次数: 0
Twenty-nine million intrinsic Q-factor monolithic microresonators on thin-film lithium niobate 铌酸锂薄膜上的 2,900 万个本征 Q 因子单片微谐振器
IF 7.6 1区 物理与天体物理 Q1 OPTICS Pub Date : 2024-05-21 DOI: 10.1364/prj.521172
Xinrui Zhu, Yaowen Hu, Shengyuan Lu, Hana K. Warner, Xudong Li, Yunxiang Song, Letícia Magalhães, Amirhassan Shams-Ansari, Andrea Cordaro, Neil Sinclair, Marko Lončar
The recent emergence of thin-film lithium niobate (TFLN) has extended the landscape of integrated photonics. This has been enabled by the commercialization of TFLN wafers and advanced nanofabrication of TFLN such as high-quality dry etching. However, fabrication imperfections still limit the propagation loss to a few dB/m, restricting the impact of this platform. Here, we demonstrate TFLN microresonators with a record-high intrinsic quality (Q) factor of twenty-nine million, corresponding to an ultra-low propagation loss of 1.3 dB/m. We present spectral analysis and the statistical distribution of Q factors across different resonator geometries. Our work pushes the fabrication limits of TFLN photonics to achieve a Q factor within 1 order of magnitude of the material limit.
最近出现的薄膜铌酸锂(TFLN)扩展了集成光子学的领域。这得益于 TFLN 晶圆的商业化和先进的 TFLN 纳米制造技术(如高质量干法蚀刻)。然而,制造缺陷仍将传播损耗限制在几 dB/m,限制了这一平台的影响力。在此,我们展示了 TFLN 微谐振器,其内在质量(Q)因子达到创纪录的 2,900 万,相当于 1.3 dB/m 的超低传播损耗。我们介绍了不同谐振器几何形状的频谱分析和 Q 因子的统计分布。我们的工作突破了 TFLN 光子技术的制造极限,使 Q 因子达到了材料极限的 1 个数量级。
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引用次数: 0
Liquid crystal immunosensors for the selective detection of Escherichia coli with a fast analysis tool 利用快速分析工具选择性检测大肠杆菌的液晶免疫传感器
IF 7.6 1区 物理与天体物理 Q1 OPTICS Pub Date : 2024-04-29 DOI: 10.1364/prj.524660
Sandro C. Oliveira, Maria S. Soares, Bárbara V. Gonçalves, Andreia C. M. Rodrigues, Amadeu M. V. M. Soares, Rita G. Sobral, Nuno F. Santos, Jan Nedoma, Pedro L. Almeida, Carlos Marques
The consumption of contaminated food may cause serious illnesses, and traditional methods to detect Escherichia coli are still associated with long waiting times and high costs given the necessity to transport samples to specialized laboratories. There is a need to develop new technologies that allow cheap, fast, and direct monitoring at the site of interest. Thus, in this work, we developed optical immunosensors for the selective detection of E. coli, based on liquid crystal technology, whose molecules can align in different manners depending on the boundary conditions (such as substrates) as well as the environment that they experience. Each glass substrate was functionalized with anti-E. coli antibody using cysteamine as an intermediate, and a vertical alignment was imposed on the liquid crystal molecules by using DMOAP during functionalization. The presence of bacteria disrupts the alignment of the liquid crystal molecules, changing the intensity of light emerging between cross polarizers, measured using a polarized optical microscope and a monochromator. It was possible to detect E. coli in suspensions in the concentration range from 2.8 cells/mL to 2.8×109 cells/mL. Selectivity was also evaluated, and the sensors were used to analyze contaminated water samples. A prototype was developed to allow faster, in-situ, and easier analysis avoiding bulky instruments.
食用受污染的食物可能会导致严重的疾病,而检测大肠杆菌的传统方法仍然需要等待很长时间,而且由于必须将样本运送到专门的实验室,成本很高。有必要开发新技术,以便在相关地点进行廉价、快速和直接的监测。因此,在这项工作中,我们基于液晶技术开发了用于选择性检测大肠杆菌的光学免疫传感器,其分子可以根据边界条件(如基板)以及它们所经历的环境以不同的方式排列。以半胱胺为中间体,用抗大肠杆菌抗体对每块玻璃基板进行功能化,并在功能化过程中使用 DMOAP 对液晶分子进行垂直排列。细菌的存在破坏了液晶分子的排列,改变了交叉偏振器之间出现的光强度。在浓度范围为 2.8 cells/mL 至 2.8×109 cells/mL 的悬浮液中,可以检测到大肠杆菌。还对选择性进行了评估,并将传感器用于分析受污染的水样。开发出的原型可以更快、更方便地进行现场分析,避免使用笨重的仪器。
{"title":"Liquid crystal immunosensors for the selective detection of Escherichia coli with a fast analysis tool","authors":"Sandro C. Oliveira, Maria S. Soares, Bárbara V. Gonçalves, Andreia C. M. Rodrigues, Amadeu M. V. M. Soares, Rita G. Sobral, Nuno F. Santos, Jan Nedoma, Pedro L. Almeida, Carlos Marques","doi":"10.1364/prj.524660","DOIUrl":"https://doi.org/10.1364/prj.524660","url":null,"abstract":"The consumption of contaminated food may cause serious illnesses, and traditional methods to detect <jats:italic toggle=\"yes\">Escherichia coli</jats:italic> are still associated with long waiting times and high costs given the necessity to transport samples to specialized laboratories. There is a need to develop new technologies that allow cheap, fast, and direct monitoring at the site of interest. Thus, in this work, we developed optical immunosensors for the selective detection of <jats:italic toggle=\"yes\">E. coli</jats:italic>, based on liquid crystal technology, whose molecules can align in different manners depending on the boundary conditions (such as substrates) as well as the environment that they experience. Each glass substrate was functionalized with anti-<jats:italic toggle=\"yes\">E. coli</jats:italic> antibody using cysteamine as an intermediate, and a vertical alignment was imposed on the liquid crystal molecules by using DMOAP during functionalization. The presence of bacteria disrupts the alignment of the liquid crystal molecules, changing the intensity of light emerging between cross polarizers, measured using a polarized optical microscope and a monochromator. It was possible to detect <jats:italic toggle=\"yes\">E. coli</jats:italic> in suspensions in the concentration range from 2.8 cells/mL to 2.8×10<jats:sup>9</jats:sup> cells/<jats:italic>mL</jats:italic>. Selectivity was also evaluated, and the sensors were used to analyze contaminated water samples. A prototype was developed to allow faster, <jats:italic toggle=\"yes\">in-situ</jats:italic>, and easier analysis avoiding bulky instruments.","PeriodicalId":20048,"journal":{"name":"Photonics Research","volume":"43 1","pages":""},"PeriodicalIF":7.6,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141869364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Tunable polarization holographic gratings obtained by varying the ratio of intensities of the recording beams 通过改变记录光束的强度比获得可调谐偏振全息光栅
IF 7.6 1区 物理与天体物理 Q1 OPTICS Pub Date : 2024-04-01 DOI: 10.1364/prj.502730
Hong Chen, Ziyao Lyu, and Changshun Wang
Polarization holography has been extensively applied in many fields, such as optical science, metrology, and biochemistry, due to its property of polarization modulation. However, the modulated polarization state of diffracted light corresponds strictly to that of incident light one by one. Here, a kind of tunable polarization holographic grating has been designed in terms of Jones matrices, and intensity-based polarization manipulation has been realized experimentally. The proposed tunable polarization holographic grating is recorded on an azobenzene liquid-crystalline film by a pair of coherent light beams with orthogonal polarization states and asymmetrically controlled intensities. It is found that the diffracted light can be actively manipulated from linearly to circularly polarized based on the light intensity of the recording holographic field when the polarization state of incident light keeps constant. Our work could enrich the field of light manipulation and holography.
偏振全息技术因其偏振调制特性而被广泛应用于光学科学、计量学和生物化学等诸多领域。然而,衍射光的调制偏振态与入射光的偏振态严格一一对应。在此,我们根据琼斯矩阵设计了一种可调谐偏振全息光栅,并在实验中实现了基于强度的偏振操控。通过一对具有正交偏振态和非对称控制强度的相干光束,在偶氮苯液晶膜上记录了所提出的可调谐偏振全息光栅。研究发现,当入射光的偏振态保持不变时,衍射光可以根据记录全息场的光强主动地从线偏振到圆偏振。我们的工作可以丰富光操纵和全息领域。
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引用次数: 0
Broadband high-efficiency plasmonic metalens with negative dispersion characteristic 具有负色散特性的宽带高效等离子金属膜
IF 7.6 1区 物理与天体物理 Q1 OPTICS Pub Date : 2024-04-01 DOI: 10.1364/prj.513990
Yong-Qiang Liu, Yong Zhu, Hongcheng Yin, Jinhai Sun, Yan Wang, and Yongxing Che
Controlling the dispersion characteristic of metasurfaces (or metalenses) along a broad bandwidth is of great importance to develop high-performance broadband metadevices. Different from traditional lenses that rely on the material refractive index along the light trajectory, metasurfaces or metalenses provide a new regime of dispersion control via a sub-wavelength metastructure, which is known as negative chromatic dispersion. However, broadband metalenses design with high-performance focusing especially with a reduced device dimension is a significant challenge in society. Here, we design, fabricate, and demonstrate a broadband high-performance diffractive-type plasmonic metalens based on a circular split-ring resonator metasurface with a relative working bandwidth of 28.6%. The metalens thickness is only 0.09λ0 (λ0 is at the central wavelength), which is much thinner than previous broadband all-dielectric metalenses. The full-wave simulation results show that both high transmissive efficiency above 80% (the maximum is even above 90%) and high average focusing efficiency above 45% (the maximum is 56%) are achieved within the entire working bandwidth of 9–12 GHz. Moreover, an average high numerical aperture of 0.7 (NA=0.7) of high-efficiency microwave metalens is obtained in the simulations. The broadband high-performance metalens is also fabricated and experimental measurements verify its much higher average focusing efficiency of 55% (the maximum is above 65% within the broad bandwidth) and a moderate high NA of 0.6. The proposed plasmonic metalens can facilitate the development of wavelength-dependent broadband diffractive devices and is also meaningful to further studies on arbitrary dispersion control in diffractive optics based on plasmonic metasurfaces.
控制超表面(或金属透镜)在宽带上的色散特性,对于开发高性能宽带元器件具有重要意义。与依靠材料折射率控制光轨迹的传统透镜不同,元表面或金属透镜通过亚波长元结构提供了一种新的色散控制机制,即负色散。然而,如何设计出具有高性能聚焦功能的宽带金属透镜,尤其是缩小器件尺寸,是当今社会面临的一项重大挑战。在此,我们设计、制造并演示了一种基于圆形劈环式谐振器元表面的宽带高性能衍射型等离子体金属膜,其相对工作带宽为 28.6%。金属膜厚度仅为 0.09λ00.09λ0(λ0λ0 为中心波长),比以往的宽带全介质金属膜薄得多。全波仿真结果表明,在 9-12 GHz 的整个工作带宽内,都实现了高于 80% 的高透射效率(最大值甚至超过 90%)和高于 45% 的高平均聚焦效率(最大值为 56%)。此外,模拟还得到了平均数值孔径为 0.7(NA=0.7NA=0.7)的高效微波金属膜。宽带高性能金属膜也已制作完成,实验测量证实其平均聚焦效率高达 55%(在宽带范围内最大值超过 65%),且 NA 值适中偏高,达到 0.6。所提出的等离子体金属膜有助于开发波长相关的宽带衍射器件,对进一步研究基于等离子体元表面的衍射光学中的任意色散控制也很有意义。
{"title":"Broadband high-efficiency plasmonic metalens with negative dispersion characteristic","authors":"Yong-Qiang Liu, Yong Zhu, Hongcheng Yin, Jinhai Sun, Yan Wang, and Yongxing Che","doi":"10.1364/prj.513990","DOIUrl":"https://doi.org/10.1364/prj.513990","url":null,"abstract":"Controlling the dispersion characteristic of metasurfaces (or metalenses) along a broad bandwidth is of great importance to develop high-performance broadband metadevices. Different from traditional lenses that rely on the material refractive index along the light trajectory, metasurfaces or metalenses provide a new regime of dispersion control via a sub-wavelength metastructure, which is known as negative chromatic dispersion. However, broadband metalenses design with high-performance focusing especially with a reduced device dimension is a significant challenge in society. Here, we design, fabricate, and demonstrate a broadband high-performance diffractive-type plasmonic metalens based on a circular split-ring resonator metasurface with a relative working bandwidth of 28.6%. The metalens thickness is only <span><span style=\"color: inherit;\"><span><span><span>0.09</span><span><span style=\"margin-right: 0.05em;\">λ</span><span style=\"vertical-align: -0.4em;\">0</span></span></span></span></span><script type=\"math/mml\"><math display=\"inline\"><mrow><mn>0.09</mn><msub><mi>λ</mi><mn>0</mn></msub></mrow></math></script></span> (<span><span style=\"color: inherit;\"><span><span><span><span style=\"margin-right: 0.05em;\">λ</span><span style=\"vertical-align: -0.4em;\">0</span></span></span></span></span><script type=\"math/mml\"><math display=\"inline\"><mrow><msub><mi>λ</mi><mn>0</mn></msub></mrow></math></script></span> is at the central wavelength), which is much thinner than previous broadband all-dielectric metalenses. The full-wave simulation results show that both high transmissive efficiency above 80% (the maximum is even above 90%) and high average focusing efficiency above 45% (the maximum is 56%) are achieved within the entire working bandwidth of 9–12 GHz. Moreover, an average high numerical aperture of 0.7 (<span><span style=\"color: inherit;\"><span><span><span>NA</span><span style=\"margin-left: 0.333em; margin-right: 0.333em;\">=</span><span>0.7</span></span></span></span><script type=\"math/mml\"><math display=\"inline\"><mrow><mi>NA</mi><mo>=</mo><mn>0.7</mn></mrow></math></script></span>) of high-efficiency microwave metalens is obtained in the simulations. The broadband high-performance metalens is also fabricated and experimental measurements verify its much higher average focusing efficiency of 55% (the maximum is above 65% within the broad bandwidth) and a moderate high NA of 0.6. The proposed plasmonic metalens can facilitate the development of wavelength-dependent broadband diffractive devices and is also meaningful to further studies on arbitrary dispersion control in diffractive optics based on plasmonic metasurfaces.","PeriodicalId":20048,"journal":{"name":"Photonics Research","volume":"81 1","pages":""},"PeriodicalIF":7.6,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140322080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Aberration correction for deformable-mirror-based remote focusing enables high-accuracy whole-cell super-resolution imaging 为基于可变形镜的远程聚焦进行像差校正,实现高精度全细胞超分辨率成像
IF 7.6 1区 物理与天体物理 Q1 OPTICS Pub Date : 2024-04-01 DOI: 10.1364/prj.514414
Wei Shi, Yingchuan He, Jianlin Wang, Lulu Zhou, Jianwei Chen, Liwei Zhou, Zeyu Xi, Zhen Wang, Ke Fang, and Yiming Li
Single-molecule localization microscopy (SMLM) enables three-dimensional (3D) investigation of nanoscale structures in biological samples, offering unique insights into their organization. However, traditional 3D super-resolution microscopy using high numerical aperture (NA) objectives is limited by imaging depth of field (DOF), restricting their practical application to relatively thin biological samples. Here, we developed a unified solution for thick sample super-resolution imaging using a deformable mirror (DM) which served for fast remote focusing, optimized point spread function (PSF) engineering, and accurate aberration correction. By effectively correcting the system aberrations introduced during remote focusing and sample aberrations at different imaging depths, we achieved high-accuracy, large DOF imaging (8 μm) of the whole-cell organelles [i.e., nuclear pore complex (NPC), microtubules, and mitochondria] with a nearly uniform resolution of approximately 35 nm across the entire cellular volume.
单分子定位显微镜(SMLM)可对生物样品中的纳米级结构进行三维(3D)研究,为了解生物样品的组织结构提供独特的视角。然而,使用高数值孔径(NA)物镜的传统三维超分辨显微镜受到成像景深(DOF)的限制,其实际应用仅限于相对较薄的生物样品。在这里,我们利用可变形镜(DM)为厚样本超分辨成像开发了一种统一的解决方案,可用于快速远程聚焦、优化点扩散函数(PSF)工程和精确像差校正。通过有效校正远程聚焦过程中引入的系统像差和不同成像深度的样本像差,我们实现了整个细胞器(即核孔复合体(NPC)、微管和线粒体)的高精度、大 DOF 成像(∼8 μm∼8 μm),整个细胞体积的分辨率几乎一致,约为 35 nm。
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引用次数: 0
High-speed impulsive stimulated Brillouin microscopy 高速脉冲刺激布里渊显微镜
IF 7.6 1区 物理与天体物理 Q1 OPTICS Pub Date : 2024-03-25 DOI: 10.1364/prj.509922
Jiarui Li, Taoran Le, Hongyuan Zhang, Haoyun Wei, and Yan Li
Brillouin microscopy, which maps the elastic modulus from the frequency shift of scattered light, has evolved to a faster speed for the investigation of rapid biomechanical changes. Impulsive stimulated Brillouin scattering (ISBS) spectroscopy has the potential to speed up measurement through the resonant amplification interaction from pulsed excitation and time-domain continuous detection. However, significant progress has not been achieved due to the limitation in signal-to-noise ratio (SNR) and the corresponding need for excessive averaging to maintain high spectral precision. Moreover, the limited spatial resolution also hinders its application in mechanical imaging. Here, by scrutinizing the SNR model, we design a high-speed ISBS microscope through multi-parameter optimization including phase, reference power, and acquisition time. Leveraging this, with the further assistance of the Matrix Pencil method for data processing, three-dimensional mechanical images are mapped under multiple contrast mechanisms for a millimeter-scale polydimethylsiloxane pattern immersed in methanol, enabling the identification of these two transparent materials without any contact or labeling. Our experimental results demonstrate the capability to maintain high spectral precision and resolution at a sub-millisecond integration time for one pixel. With a two-order improvement in the speed and a tenfold improvement in the spatial resolution over the state-of-the-art systems, this method makes it possible for ISBS microscopes to sensitively investigate rapid mechanical changes in time and space.
布里渊显微镜通过散射光的频移绘制弹性模量图,已发展到更快的速度,用于研究快速的生物力学变化。脉冲激发布里渊散射(ISBS)光谱法通过脉冲激发和时域连续检测的共振放大相互作用,有可能加快测量速度。然而,由于信噪比(SNR)的限制,以及为保持高光谱精度而需要过度平均的相应需求,该技术尚未取得重大进展。此外,有限的空间分辨率也阻碍了其在机械成像中的应用。在此,我们通过对信噪比模型的仔细研究,对相位、参考功率和采集时间等多参数进行优化,设计出一种高速 ISBS 显微镜。在此基础上,我们进一步利用矩阵铅笔方法进行数据处理,在多种对比机制下绘制出了浸入甲醇中的毫米级聚二甲基硅氧烷图案的三维机械图像,从而无需任何接触或标记即可识别这两种透明材料。我们的实验结果表明,在一个像素的亚毫秒积分时间内,能够保持较高的光谱精度和分辨率。与最先进的系统相比,这种方法的速度提高了两个等级,空间分辨率提高了十倍,使 ISBS 显微镜能够灵敏地研究时间和空间的快速机械变化。
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引用次数: 0
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Photonics Research
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