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Phylogenetic Structure of a Rare Plant Community in Houhe Nature Reserve over Time and Space 后河自然保护区珍稀植物群落系统发育结构的时空特征
Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.50454
K. Zhang, Dachuan Bao, Yili Guo, Zhijun Lu, Handong Huang, M. Jiang
The phylogenetic structure of a community is composed of all genetic relationships between species across the community. Understanding the phylogenetic structure of a community will help reveal the relative importance of habitat filteringꎬ competitive exclusion and the stochastic processes constructing the community. We studied a rare plant community in a 1 hm permanent plot in Houhe Nature Reserveꎬ Hubei Province. We examined the effects of spatial scales (5 m×5 mꎬ 10 m×10 mꎬ 20 m×20 mꎬ 30 m×30 mꎬ 40 m×40 mꎬ and 50 m× 50 m) and DBH classes (5 cm≤DBH<10 cmꎬ 10 cm≤DBH<15cmꎬ 15 cm≤DBH<20 cmꎬ and DBH≥20 cm) at spatial scales (10 m ×10 mꎬ 20 m×20 mꎬ 30 m ×30 mꎬ 40 m ×40 mꎬ and 50 m×50 m) on phylogenetic structure of the community in a subtropical evergreen and deciduous broadleaf mixed forest. The results demonstrated thatꎬ with increasing spatial scaleꎬ the phylogenetic structure tended to cluster. The degree of phylogenetic clustering decreased with increasing DBH classes. Howeverꎬ the degree of phylogenetic clustering was very high at the scale of DBH≥20 cm. The phylogenetic structures of the communities were random at the scale of 25 m and clustered at scales of 100 m and largerꎬ indicating that neutrality may play a leading role at small scales and niche processes may be more important at large scales for community assembly in subtropical evergreen and deciduous broadleaf mixed forest in this case.
一个群落的系统发育结构是由整个群落中物种之间的所有遗传关系组成的。了解群落的系统发育结构将有助于揭示生境过滤ꎬ竞争排斥和构建群落的随机过程的相对重要性。研究了湖北省后河自然保护区1 hm永久样地的一个珍稀植物群落。我们检查了空间尺度上的影响(5 m×5 mꎬ10 m×10 mꎬ20 m×20 mꎬ30 m×30 mꎬ40 m×40 mꎬ50 m×50米)和胸径类(5厘米≤胸径< 10厘米ꎬ10厘米≤胸径< 15厘米ꎬ15厘米≤胸径< 20厘米ꎬ和胸径≥20厘米)在空间尺度上(10 m×10 mꎬ20 m×20 mꎬ30 m×30 mꎬ40 m×40 mꎬ50 m×50 m)系统结构的社区亚热带常绿、落叶阔叶混交林。结果表明:ꎬ随着空间尺度的增大ꎬ系统发育结构呈聚类趋势;系统发育聚类程度随胸径分级的增加而降低。但ꎬ在胸径≥20 cm尺度上,系统发育聚类程度非常高。群落的系统发育结构在25 m尺度上是随机的,而在100 m及以上尺度上是聚集性的ꎬ,这表明在亚热带常绿落叶阔叶混交林中,中性可能在小尺度上起主导作用,而生态位过程在大尺度上对群落聚集更为重要。
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引用次数: 0
ISSR Analysis on Genetic Diversity of Medically Important Lycium ruthenicum Murr. in Xinjiang 药用枸杞遗传多样性的ISSR分析。在新疆
Pub Date : 2013-01-01 DOI: 10.3724/SP.J.1142.2013.50517
Qimike Alitong, Qing‐Feng Wang, Chun-Feng Yang, Jin‐Ming Chen
Lycium ruthenicum(Solanaceae)is an important medicinal plant mainly distributed in Northwestern China.In recent years,however,the distribution range of this species has been reduced greatly due to human overexploitation and deterioration of its habitat.Studying the population genetics of this species is essential for the conservation and sustainable utilization of the plant germplasm resource.In this study,we used seven ISSR markers to measure genetic diversity of 115individuals from eight natural populations of L.ruthenicumin Xinjiang(six populations)and Gansu(two populations).A total of 64DNA fragments were scored,and 78.1%of polymorphic loci(PPL)found.High level of genetic diversity was detected in L.ruthenicum(H=0.29;Ⅰ=0.43).AMOVA analysis indicated that most variance(77.0%)resided within populations,with only a small proportion of total variation residing between populations(23%,FST=0.23).Results of the Mantel test(r=0.3602,p=0.910)and UPGMA cluster of the Xinjiang populations indicated that there was no significant relationship between geographical distance and genetic distance.UPGMA cluster of all sampled individuals showed that individuals from each population did not cluster together.The probable causes that have contributed to the current pattern of genetic structure were discussed and suggestions on future protection of the L.ruthenicumfrom southern Xinjiang were also given.
枸杞是主要分布在中国西北地区的重要药用植物。然而,近年来由于人类的过度开发和生境的恶化,该物种的分布范围大大缩小。研究该物种的群体遗传学对植物种质资源的保护和可持续利用具有重要意义。本研究利用7个ISSR标记,对新疆(6个)和甘肃(2个)芦竹天然居群的115个个体进行了遗传多样性测定。共检测到64个dna片段,发现78.1%的多态性位点(PPL)。鲁thenicum具有较高的遗传多样性(H=0.29,Ⅰ=0.43),amova分析表明,大部分变异(77.0%)存在于群体内,种群间变异仅占总变异的23% (FST=0.23)。新疆种群的Mantel检验(r=0.3602,p=0.910)和UPGMA聚类结果表明,地理距离与遗传距离无显著相关。所有样本个体的UPGMA聚类表明,每个种群的个体没有聚在一起。讨论了形成目前遗传结构格局的可能原因,并对今后的保护提出了建议。
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引用次数: 2
Ultrastructural Observation on Embryo Sac Development inPhaius tankervilliae(Aiton)Bl. 褐褐phaius tankervillae (Aiton)Bl胚囊发育的超微结构观察。
Pub Date : 2012-01-01 DOI: 10.3724/SP.J.1142.2012.20188
Dong-Mei Li, Chenggang Wu, X. Ye, C. Liang
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引用次数: 1
Optimization and Establishment of SRAP-PCR in Lotus 荷花SRAP-PCR的优化与建立
Pub Date : 2012-01-01 DOI: 10.3724/SP.J.1142.2012.10085
Mei Yang, Liming Xu, Yan-Ling Liu
We established an advanced SRAP-PCR reaction system for Chinese Antique Lotus and American Lotus using a single factor experiment with five impact factors,including DNA template,Mg2+,dNTP mixture,Taq DNA polymerase and primer.The 10 μL reaction mixture contained 50 ng of genomic DNA template,1 μL of 10×Buffer,2 mmol/L of MgCl2,0.20 mmol/L of dNTP,0.75 U of Taq DNA polymerase,and 0.8 μmol/L of primers.To test the stability of the optimized SRAP-PCR system,the PCR was further amplified in the core-collection of flower lotus with 88 cultivars using 16 primer pairs.A total of 183 clear bands were obtained throughout all materials,of which 165(90%) bands were polymorphic.Therefore,the established SRAP reaction system for lotus was reliable.The results provided technical support for evaluating genetic diversity as well as constructing genetic linkage maps and molecular marker-assisted breeding of lotus.
采用DNA模板、Mg2+、dNTP混合物、Taq DNA聚合酶和引物5个影响因子的单因素实验,建立了中国古荷花和美洲荷花的先进的SRAP-PCR反应体系。10 μL的反应混合物中含有50 ng基因组DNA模板、1 μL 10×Buffer、2 mmol/L MgCl2、0.20 mmol/L dNTP、0.75 U Taq DNA聚合酶和0.8 μmol/L引物。为了验证优化后的SRAP-PCR体系的稳定性,利用16对引物在88个品种的花莲花核心样本中进一步扩增。在所有材料中共获得183条清晰条带,其中165条(90%)为多态条带。因此,所建立的莲花SRAP反应体系是可靠的。研究结果为荷花遗传多样性评价、遗传连锁图谱构建和分子标记辅助育种提供了技术支持。
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引用次数: 0
Diversity of Aquatic Vascular Plants in the Yangtze Delta 长江三角洲水生维管植物的多样性
Pub Date : 2012-01-01 DOI: 10.3724/SP.J.1142.2012.30238
Yuexin Zhang, Guangfu Zhang, Yue Li, Ling-Ling Li, Lipeng Yu
Based on field investigation and literature survey,the species composition,geographical elements,and endangered state of aquatic vascular plants in the Yangtze Delta were analyzed.We found 185 wild species belonging to 90 genera and 39 families in this area,most of which were distributed in habitats such as ditches,rice-fields,and ponds.Additionally,emergent plants were the main type in terms of life form.This flora was chiefly temperate in nature according to areal-type analysis both at the level of genera and of species.In light of IUCN Red List Categories and Criteria(Edition 3.1),those species were classified into 9 types,including Regionally Extinct(RE) 1,Critically Endangered(CR) 3,Endangered(EN) 8,and Vulnerable(VU) 10.Finally,we also examined the main reasons causing these aquatic plants to become threatened.
在野外调查和文献调查的基础上,对长江三角洲水生维管植物的种类组成、地理要素和濒危状况进行了分析。在该地区共发现野生种185种,隶属于39科90属,主要分布在沟渠、稻田、池塘等生境。此外,在生命形式方面,新兴植物是主要类型。从属和种的面积型分析来看,该植物区系以温带为主。根据世界自然保护联盟(IUCN)红色名录分类标准(3.1版),将这些物种分为9个类型,包括区域灭绝(RE) 1、极度濒危(CR) 3、濒危(EN) 8和易危(VU) 10。最后,我们还研究了导致这些水生植物受到威胁的主要原因。
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引用次数: 2
Current Status and Conservation Strategies of Wild Orchid Resources in Guangxi Yachang Forests 广西雅昌林区野生兰花资源现状及保护对策
Pub Date : 2012-01-01 DOI: 10.3724/SP.J.1142.2012.30285
Chang-Lin Feng, Zhenping Deng, D. Cai, TianChang Wu, Hongyan Jia, L. Bai, Zulun Zhao, Yong-zhong Su
The Yachang forest region is rich in wild orchid diversity,with 156 orchid species belonging to 52 genera distributed in the hills throughout the Yachang Orchid Nature Reserve.The orchids' distribution in the Yachang region is,however,uneven and naturally or unnaturally fragmented.In particular,there are sixteen distribution hotspots with large concentrations of orchid species and populations within the region.The continuing existence of these rich wild orchid resources are threatened by disturbance from human activities,livestock grazing,and other fast-changing environmental conditions within the Yachang region.We recommend that the conservation strategies of the wild orchids in Yachang should include primarily of in-situ conservation measures,supplemented by ex-situ conservation.Yachang Orchid Nature Reserve should complete and improve its protection facilities as soon as possible,including the completion of a wild orchid germplasm bank,construction of fences around orchid-rich areas that are subject to heavy herbivory from livestock,and intensification of foot-patrols in these areas.
崖昌林区野生兰花种类丰富,共有52属156种,分布在整个崖昌兰花自然保护区的丘陵地带。然而,雅昌地区兰花的分布是不均匀的,自然或非自然破碎。特别是,该地区有16个兰花种类和种群大量集中的分布热点。雅昌地区丰富的野生兰花资源的持续生存受到人类活动、牲畜放牧和其他快速变化的环境条件的干扰。建议雅昌野生兰科植物的保护策略应以原地保护为主,迁地保护为辅。雅昌兰花自然保护区应尽快完善其保护设施,包括建成野生兰花种质资源库,在家畜大量食草的兰花富集区周围修建围栏,并加强对这些地区的徒步巡逻。
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引用次数: 3
Evaluation on the Application Value of Identification in Cucurbitaceae by ITS2 ITS2在葫芦科植物鉴定中的应用价值评价
Pub Date : 2012-01-01 DOI: 10.3724/SP.J.1142.2012.60631
Yin-lin Tang, Y. Liu, H. Yao, Zhendong Chen, Yaosheng Wu, Penghong Xu, Dongping Jiang
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引用次数: 0
Relationships between Geographical Distribution of EndangeredPinus kwangtungensisand Climate in China 中国濒危光东松地理分布与气候的关系
Pub Date : 2012-01-01 DOI: 10.3724/SP.J.1142.2012.60577
C. Tao, Xiao-xiao Li, Qing-chun Wang, Guo-fa Cui
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引用次数: 2
Purification of Polysaccharide from the Laminae of Elaeagnus mollis Diels with Chitosan Flocculation 壳聚糖絮凝法纯化白菖蒲叶多糖
Pub Date : 2012-01-01 DOI: 10.3724/SP.J.1142.2012.60639
Jinghai Zhao, Jun-ping Liu, Xiaochen Sun, Xiao-Jun Zhang, Zhi‐Feng Zhang, Guiwen Yan
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引用次数: 0
Seed Micromorphological Characteristics ofEuryaand Their Systematic Implications 植物种子微形态特征及其系统意义
Pub Date : 2012-01-01 DOI: 10.3724/SP.J.1142.2011.20107
Xiang-gang Shi, Kaiyan Li, Xiaoling Song, C. Ye
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引用次数: 1
期刊
Plant Science Journal
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