Pub Date : 2013-01-01DOI: 10.3724/SP.J.1142.2013.50454
K. Zhang, Dachuan Bao, Yili Guo, Zhijun Lu, Handong Huang, M. Jiang
The phylogenetic structure of a community is composed of all genetic relationships between species across the community. Understanding the phylogenetic structure of a community will help reveal the relative importance of habitat filteringꎬ competitive exclusion and the stochastic processes constructing the community. We studied a rare plant community in a 1 hm permanent plot in Houhe Nature Reserveꎬ Hubei Province. We examined the effects of spatial scales (5 m×5 mꎬ 10 m×10 mꎬ 20 m×20 mꎬ 30 m×30 mꎬ 40 m×40 mꎬ and 50 m× 50 m) and DBH classes (5 cm≤DBH<10 cmꎬ 10 cm≤DBH<15cmꎬ 15 cm≤DBH<20 cmꎬ and DBH≥20 cm) at spatial scales (10 m ×10 mꎬ 20 m×20 mꎬ 30 m ×30 mꎬ 40 m ×40 mꎬ and 50 m×50 m) on phylogenetic structure of the community in a subtropical evergreen and deciduous broadleaf mixed forest. The results demonstrated thatꎬ with increasing spatial scaleꎬ the phylogenetic structure tended to cluster. The degree of phylogenetic clustering decreased with increasing DBH classes. Howeverꎬ the degree of phylogenetic clustering was very high at the scale of DBH≥20 cm. The phylogenetic structures of the communities were random at the scale of 25 m and clustered at scales of 100 m and largerꎬ indicating that neutrality may play a leading role at small scales and niche processes may be more important at large scales for community assembly in subtropical evergreen and deciduous broadleaf mixed forest in this case.
{"title":"Phylogenetic Structure of a Rare Plant Community in Houhe Nature Reserve over Time and Space","authors":"K. Zhang, Dachuan Bao, Yili Guo, Zhijun Lu, Handong Huang, M. Jiang","doi":"10.3724/SP.J.1142.2013.50454","DOIUrl":"https://doi.org/10.3724/SP.J.1142.2013.50454","url":null,"abstract":"The phylogenetic structure of a community is composed of all genetic relationships between species across the community. Understanding the phylogenetic structure of a community will help reveal the relative importance of habitat filteringꎬ competitive exclusion and the stochastic processes constructing the community. We studied a rare plant community in a 1 hm permanent plot in Houhe Nature Reserveꎬ Hubei Province. We examined the effects of spatial scales (5 m×5 mꎬ 10 m×10 mꎬ 20 m×20 mꎬ 30 m×30 mꎬ 40 m×40 mꎬ and 50 m× 50 m) and DBH classes (5 cm≤DBH<10 cmꎬ 10 cm≤DBH<15cmꎬ 15 cm≤DBH<20 cmꎬ and DBH≥20 cm) at spatial scales (10 m ×10 mꎬ 20 m×20 mꎬ 30 m ×30 mꎬ 40 m ×40 mꎬ and 50 m×50 m) on phylogenetic structure of the community in a subtropical evergreen and deciduous broadleaf mixed forest. The results demonstrated thatꎬ with increasing spatial scaleꎬ the phylogenetic structure tended to cluster. The degree of phylogenetic clustering decreased with increasing DBH classes. Howeverꎬ the degree of phylogenetic clustering was very high at the scale of DBH≥20 cm. The phylogenetic structures of the communities were random at the scale of 25 m and clustered at scales of 100 m and largerꎬ indicating that neutrality may play a leading role at small scales and niche processes may be more important at large scales for community assembly in subtropical evergreen and deciduous broadleaf mixed forest in this case.","PeriodicalId":20134,"journal":{"name":"Plant Science Journal","volume":"31 1","pages":"454"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69789295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lycium ruthenicum(Solanaceae)is an important medicinal plant mainly distributed in Northwestern China.In recent years,however,the distribution range of this species has been reduced greatly due to human overexploitation and deterioration of its habitat.Studying the population genetics of this species is essential for the conservation and sustainable utilization of the plant germplasm resource.In this study,we used seven ISSR markers to measure genetic diversity of 115individuals from eight natural populations of L.ruthenicumin Xinjiang(six populations)and Gansu(two populations).A total of 64DNA fragments were scored,and 78.1%of polymorphic loci(PPL)found.High level of genetic diversity was detected in L.ruthenicum(H=0.29;Ⅰ=0.43).AMOVA analysis indicated that most variance(77.0%)resided within populations,with only a small proportion of total variation residing between populations(23%,FST=0.23).Results of the Mantel test(r=0.3602,p=0.910)and UPGMA cluster of the Xinjiang populations indicated that there was no significant relationship between geographical distance and genetic distance.UPGMA cluster of all sampled individuals showed that individuals from each population did not cluster together.The probable causes that have contributed to the current pattern of genetic structure were discussed and suggestions on future protection of the L.ruthenicumfrom southern Xinjiang were also given.
{"title":"ISSR Analysis on Genetic Diversity of Medically Important Lycium ruthenicum Murr. in Xinjiang","authors":"Qimike Alitong, Qing‐Feng Wang, Chun-Feng Yang, Jin‐Ming Chen","doi":"10.3724/SP.J.1142.2013.50517","DOIUrl":"https://doi.org/10.3724/SP.J.1142.2013.50517","url":null,"abstract":"Lycium ruthenicum(Solanaceae)is an important medicinal plant mainly distributed in Northwestern China.In recent years,however,the distribution range of this species has been reduced greatly due to human overexploitation and deterioration of its habitat.Studying the population genetics of this species is essential for the conservation and sustainable utilization of the plant germplasm resource.In this study,we used seven ISSR markers to measure genetic diversity of 115individuals from eight natural populations of L.ruthenicumin Xinjiang(six populations)and Gansu(two populations).A total of 64DNA fragments were scored,and 78.1%of polymorphic loci(PPL)found.High level of genetic diversity was detected in L.ruthenicum(H=0.29;Ⅰ=0.43).AMOVA analysis indicated that most variance(77.0%)resided within populations,with only a small proportion of total variation residing between populations(23%,FST=0.23).Results of the Mantel test(r=0.3602,p=0.910)and UPGMA cluster of the Xinjiang populations indicated that there was no significant relationship between geographical distance and genetic distance.UPGMA cluster of all sampled individuals showed that individuals from each population did not cluster together.The probable causes that have contributed to the current pattern of genetic structure were discussed and suggestions on future protection of the L.ruthenicumfrom southern Xinjiang were also given.","PeriodicalId":20134,"journal":{"name":"Plant Science Journal","volume":"31 1","pages":"517"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69789459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-01-01DOI: 10.3724/SP.J.1142.2012.20188
Dong-Mei Li, Chenggang Wu, X. Ye, C. Liang
{"title":"Ultrastructural Observation on Embryo Sac Development inPhaius tankervilliae(Aiton)Bl.","authors":"Dong-Mei Li, Chenggang Wu, X. Ye, C. Liang","doi":"10.3724/SP.J.1142.2012.20188","DOIUrl":"https://doi.org/10.3724/SP.J.1142.2012.20188","url":null,"abstract":"","PeriodicalId":20134,"journal":{"name":"Plant Science Journal","volume":"30 1","pages":"188"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69786233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-01-01DOI: 10.3724/SP.J.1142.2012.10085
Mei Yang, Liming Xu, Yan-Ling Liu
We established an advanced SRAP-PCR reaction system for Chinese Antique Lotus and American Lotus using a single factor experiment with five impact factors,including DNA template,Mg2+,dNTP mixture,Taq DNA polymerase and primer.The 10 μL reaction mixture contained 50 ng of genomic DNA template,1 μL of 10×Buffer,2 mmol/L of MgCl2,0.20 mmol/L of dNTP,0.75 U of Taq DNA polymerase,and 0.8 μmol/L of primers.To test the stability of the optimized SRAP-PCR system,the PCR was further amplified in the core-collection of flower lotus with 88 cultivars using 16 primer pairs.A total of 183 clear bands were obtained throughout all materials,of which 165(90%) bands were polymorphic.Therefore,the established SRAP reaction system for lotus was reliable.The results provided technical support for evaluating genetic diversity as well as constructing genetic linkage maps and molecular marker-assisted breeding of lotus.
{"title":"Optimization and Establishment of SRAP-PCR in Lotus","authors":"Mei Yang, Liming Xu, Yan-Ling Liu","doi":"10.3724/SP.J.1142.2012.10085","DOIUrl":"https://doi.org/10.3724/SP.J.1142.2012.10085","url":null,"abstract":"We established an advanced SRAP-PCR reaction system for Chinese Antique Lotus and American Lotus using a single factor experiment with five impact factors,including DNA template,Mg2+,dNTP mixture,Taq DNA polymerase and primer.The 10 μL reaction mixture contained 50 ng of genomic DNA template,1 μL of 10×Buffer,2 mmol/L of MgCl2,0.20 mmol/L of dNTP,0.75 U of Taq DNA polymerase,and 0.8 μmol/L of primers.To test the stability of the optimized SRAP-PCR system,the PCR was further amplified in the core-collection of flower lotus with 88 cultivars using 16 primer pairs.A total of 183 clear bands were obtained throughout all materials,of which 165(90%) bands were polymorphic.Therefore,the established SRAP reaction system for lotus was reliable.The results provided technical support for evaluating genetic diversity as well as constructing genetic linkage maps and molecular marker-assisted breeding of lotus.","PeriodicalId":20134,"journal":{"name":"Plant Science Journal","volume":"29 1","pages":"85"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69786331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Based on field investigation and literature survey,the species composition,geographical elements,and endangered state of aquatic vascular plants in the Yangtze Delta were analyzed.We found 185 wild species belonging to 90 genera and 39 families in this area,most of which were distributed in habitats such as ditches,rice-fields,and ponds.Additionally,emergent plants were the main type in terms of life form.This flora was chiefly temperate in nature according to areal-type analysis both at the level of genera and of species.In light of IUCN Red List Categories and Criteria(Edition 3.1),those species were classified into 9 types,including Regionally Extinct(RE) 1,Critically Endangered(CR) 3,Endangered(EN) 8,and Vulnerable(VU) 10.Finally,we also examined the main reasons causing these aquatic plants to become threatened.
{"title":"Diversity of Aquatic Vascular Plants in the Yangtze Delta","authors":"Yuexin Zhang, Guangfu Zhang, Yue Li, Ling-Ling Li, Lipeng Yu","doi":"10.3724/SP.J.1142.2012.30238","DOIUrl":"https://doi.org/10.3724/SP.J.1142.2012.30238","url":null,"abstract":"Based on field investigation and literature survey,the species composition,geographical elements,and endangered state of aquatic vascular plants in the Yangtze Delta were analyzed.We found 185 wild species belonging to 90 genera and 39 families in this area,most of which were distributed in habitats such as ditches,rice-fields,and ponds.Additionally,emergent plants were the main type in terms of life form.This flora was chiefly temperate in nature according to areal-type analysis both at the level of genera and of species.In light of IUCN Red List Categories and Criteria(Edition 3.1),those species were classified into 9 types,including Regionally Extinct(RE) 1,Critically Endangered(CR) 3,Endangered(EN) 8,and Vulnerable(VU) 10.Finally,we also examined the main reasons causing these aquatic plants to become threatened.","PeriodicalId":20134,"journal":{"name":"Plant Science Journal","volume":"67 4 1","pages":"238"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69786525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-01-01DOI: 10.3724/SP.J.1142.2012.30285
Chang-Lin Feng, Zhenping Deng, D. Cai, TianChang Wu, Hongyan Jia, L. Bai, Zulun Zhao, Yong-zhong Su
The Yachang forest region is rich in wild orchid diversity,with 156 orchid species belonging to 52 genera distributed in the hills throughout the Yachang Orchid Nature Reserve.The orchids' distribution in the Yachang region is,however,uneven and naturally or unnaturally fragmented.In particular,there are sixteen distribution hotspots with large concentrations of orchid species and populations within the region.The continuing existence of these rich wild orchid resources are threatened by disturbance from human activities,livestock grazing,and other fast-changing environmental conditions within the Yachang region.We recommend that the conservation strategies of the wild orchids in Yachang should include primarily of in-situ conservation measures,supplemented by ex-situ conservation.Yachang Orchid Nature Reserve should complete and improve its protection facilities as soon as possible,including the completion of a wild orchid germplasm bank,construction of fences around orchid-rich areas that are subject to heavy herbivory from livestock,and intensification of foot-patrols in these areas.
{"title":"Current Status and Conservation Strategies of Wild Orchid Resources in Guangxi Yachang Forests","authors":"Chang-Lin Feng, Zhenping Deng, D. Cai, TianChang Wu, Hongyan Jia, L. Bai, Zulun Zhao, Yong-zhong Su","doi":"10.3724/SP.J.1142.2012.30285","DOIUrl":"https://doi.org/10.3724/SP.J.1142.2012.30285","url":null,"abstract":"The Yachang forest region is rich in wild orchid diversity,with 156 orchid species belonging to 52 genera distributed in the hills throughout the Yachang Orchid Nature Reserve.The orchids' distribution in the Yachang region is,however,uneven and naturally or unnaturally fragmented.In particular,there are sixteen distribution hotspots with large concentrations of orchid species and populations within the region.The continuing existence of these rich wild orchid resources are threatened by disturbance from human activities,livestock grazing,and other fast-changing environmental conditions within the Yachang region.We recommend that the conservation strategies of the wild orchids in Yachang should include primarily of in-situ conservation measures,supplemented by ex-situ conservation.Yachang Orchid Nature Reserve should complete and improve its protection facilities as soon as possible,including the completion of a wild orchid germplasm bank,construction of fences around orchid-rich areas that are subject to heavy herbivory from livestock,and intensification of foot-patrols in these areas.","PeriodicalId":20134,"journal":{"name":"Plant Science Journal","volume":"9 1","pages":"285"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69787142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2012-01-01DOI: 10.3724/SP.J.1142.2012.60631
Yin-lin Tang, Y. Liu, H. Yao, Zhendong Chen, Yaosheng Wu, Penghong Xu, Dongping Jiang
{"title":"Evaluation on the Application Value of Identification in Cucurbitaceae by ITS2","authors":"Yin-lin Tang, Y. Liu, H. Yao, Zhendong Chen, Yaosheng Wu, Penghong Xu, Dongping Jiang","doi":"10.3724/SP.J.1142.2012.60631","DOIUrl":"https://doi.org/10.3724/SP.J.1142.2012.60631","url":null,"abstract":"","PeriodicalId":20134,"journal":{"name":"Plant Science Journal","volume":"30 1","pages":"631"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69787488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}