Plant Physiology and Biochemistry最新文献

{"title":"The VvDREB2A-VvGolS3 regulatory module enhances cold tolerance in Vitis vinifera L","authors":"Lixia Hou ,&nbsp;Huiting Chen ,&nbsp;Wei Yan ,&nbsp;Yaoyao Zhang ,&nbsp;Meng Li,&nbsp;Qiqi Wu,&nbsp;Mengfei Zhang,&nbsp;Guangchao Liu,&nbsp;Qing Ye,&nbsp;Xin Liu","doi":"10.1016/j.plaphy.2025.110821","DOIUrl":"10.1016/j.plaphy.2025.110821","url":null,"abstract":"<div><div>Galactinol synthase (GolS), a crucial enzyme in the synthesis pathway of raffinose family oligosaccharides (RFOs), plays a vital role in plants against abiotic stress. In previous experiments, <em>VvGolS3</em> cloned from the grape variety ‘Zuoyouhong’ exhibited cold-inducible expression. However, its specific function and regulatory mechanism remained largely unknown. Additionally, our research has shown that the transcription factor VvDREB2A was involved in grape response to cold stress, yet its molecular interaction with <em>VvGolS3</em> required further investigation. In this study, it was observed that the expression level of <em>VvGolS3</em> was significantly higher in cold-resistant grape varieties compared to sensitive ones. Overexpression of <em>VvGolS3</em> in transgenic <em>Arabidopsis</em> resulted in enhanced cold tolerance, as evidenced by reduced levels of reactive oxygen species (ROS) and increased RFOs content under cold stress conditions. Transient overexpression of <em>VvGolS3</em> in grapevine leaves also conferred enhanced cold tolerance. Furthermore, overexpressing-<em>VvDREB2A</em> grape calli showed increased cold tolerance and higher levels of RFOs. Similarly, transient overexpression of VvDREB2A in grapevine leaves enhanced cold tolerance. Through yeast one-hybrid (Y1H), electrophoretic mobility shift assay (EMSA), and dual-luciferase reporter system, it was confirmed that VvDREB2A directly binds to the <em>VvGolS3</em> promoter and promotes its expression. Collectively, these findings indicate that VvDREB2A directly targetes and regulates the expression of <em>VvGolS3</em>, thereby promoting the accumulation of RFOs and enhancing antioxidant enzymes activity, and ultimately improving cold stress tolerance. This study provides the first mechanistic insight into <em>VvGolS3</em>-mediated cold resistance and identifies a novel genetic module (VvDREB2A-<em>VvGolS3</em>) for molecular breeding of cold-tolerant grape cultivars.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110821"},"PeriodicalIF":5.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145655261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antarctic Mokoshia rubra and Mokoshia mucilaginosa enhance growth and cadmium tolerance in Nicotiana species","authors":"Syed Inzimam Ul Haq ,&nbsp;Josef Hájek ,&nbsp;Ivana Mašlaňová ,&nbsp;Ivo Sedláček ,&nbsp;Miloš Barták","doi":"10.1016/j.plaphy.2025.110808","DOIUrl":"10.1016/j.plaphy.2025.110808","url":null,"abstract":"<div><div>Cadmium (Cd) contamination severely limits plant growth and agricultural productivity, emphasizing the need for sustainable approaches to mitigate heavy metal stress. This study assessed the plant growth–promoting potential of two Antarctic bacterial strains, <em>Mokoshia rubra</em> and <em>M</em>. <em>mucilaginosa</em>, in enhancing growth and Cd tolerance of <em>Nicotiana tabacum</em> and <em>N</em>. <em>benthamiana</em>. Both strains exhibited strong Cd resistance and key plant growth–promoting traits, including indole-3-acetic acid (IAA) production, phosphate solubilization, siderophore secretion, and nitrogen fixation. Inoculation with <em>M</em>. <em>rubra</em> or <em>M</em>. <em>mucilaginosa</em> mitigated Cd-induced declines in biomass, photosynthetic performance, and antioxidant capacity. Improvements in chlorophyll fluorescence parameters related to photosystem II (PSII) efficiency and in leaf spectral vitality indices indicated enhanced physiological stability under stress. Biochemical analyses further revealed that inoculated plants maintained higher chlorophyll, carotenoid, soluble sugar, and protein levels while limiting excessive accumulation of proline and total phenols. Activities of antioxidant enzymes—superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX)—were markedly elevated, contributing to effective reactive oxygen species (ROS) detoxification. Overall, <em>Mokoshia</em> strains significantly improved Cd stress tolerance in <em>Nicotiana</em> species, highlighting their potential as eco-friendly bioinoculants for sustainable crop production in metal-contaminated soils.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110808"},"PeriodicalIF":5.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145661786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PbNAC155 confers salt stress tolerance through ABA signalling pathway in ‘duli’ pear (Pyrus betulifolia Bunge)","authors":"Rui Liu ,&nbsp;Ziyi Li ,&nbsp;Qingnan Li ,&nbsp;Yuyue Zhang ,&nbsp;Haibo Jiang ,&nbsp;Huaixuan Wang ,&nbsp;Hao Zhang ,&nbsp;Hui Ma ,&nbsp;Haixia Zhang ,&nbsp;Yuxing Zhang ,&nbsp;Jianfeng Xu","doi":"10.1016/j.plaphy.2025.110820","DOIUrl":"10.1016/j.plaphy.2025.110820","url":null,"abstract":"<div><div>The NAC transcription factors form a major group of plant-specific family instrumental in both developmental processes and abiotic stress adaptation. The role of NAC genes in salt stress response remains unclear. In this study, 178 PbNAC genes, designated as <em>PbNACs</em>, were procured from the ‘duli’ pear (<em>Pyrus betul</em><em>i</em><em>folia</em> Bunge) genome, among which <em>PbNAC155</em> was salt- and ABA-responsive. Functional characterization showed that overexpression of <em>PbNAC155</em> significantly enhanced salt tolerance in pear calli, whereas its knockdown increased salt sensitivity. Protein-protein interaction assays, namely Y2H and BiFC, demonstrated that PbNAC155 interacts with PbABI5, a core transcription regulator for ABA signalling, to form a complex that cooperatively regulates salt tolerance. Furthermore, we observed that <em>PbNAC155</em> binds to the <em>PbMYB88</em> promoter primarily based on Y1H assay, thereby setting up a new regulatory module that controls the salt stress adaptation process. Collectively, our findings indicate that the PbNAC155-PbABI5 complex acts cooperatively to activate its downstream target gene, PbMYB88, and thus elucidates a novel mechanism for salt tolerance via the ABA signalling pathway.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110820"},"PeriodicalIF":5.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145669358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Allantoin enhances growth and nutrient accumulation in Dioscorea opposita under saline-alkali stress through regulation of ion homeostasis and antioxidant capacity","authors":"Chaochuang Li ,&nbsp;Shunpeng Li ,&nbsp;Longlong Ma ,&nbsp;Houmin Wang ,&nbsp;Xuanzhang Li ,&nbsp;Mingjun Li ,&nbsp;Qingxiang Yang","doi":"10.1016/j.plaphy.2025.110819","DOIUrl":"10.1016/j.plaphy.2025.110819","url":null,"abstract":"<div><div>Saline-alkali stress (SAS) significantly impairs crop growth, yield and quality, while allantoin plays a crucial role in enhancing plant tolerance to this stress. Yam (<em>Dioscorea opposita</em> Thunb.) has substantial nutritional and medicinal value. However, the regulatory mechanism of allantoin in regulating yam growth and nutritional quality under SAS remains largely unclear. In this study, we found that SAS severely inhibited the growth and root development of yam bulbil seedlings. Specifically, in leaves, the contents of osmotic regulators (e.g., proline, soluble sugar) and malondialdehyde (MDA), along with the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), were significantly increased. Additionally, leaf Na⁺ content was increased, K⁺ content was decreased, and the Na⁺/K⁺ ratio was significantly elevated. Meanwhile, the contents of allantoin, diosgenin, and polysaccharides in tubers were significantly increased. Overexpression of the allantoin synthase gene <em>DoAS</em> in <em>Arabidopsis thaliana</em> further enhanced its tolerance to SAS. Furthermore, compared with the SAS-only group, allantoin treatment significantly improved yam seedling growth, reduced leaf proline and MDA contents, enhanced SOD and POD activities, decreased the Na⁺/K⁺ ratio, increased tuber yield and contents of major active components, and lowered tuber Na⁺ content. Taken together, allantoin significantly improves ionic balance and antioxidant capacity in yam bulbil seedlings under SAS, thereby promoting seedling growth and nutrient accumulation in tubers. This study thus highlights the critical role of allantoin in regulating the growth and nutrient accumulation in tuber crops under SAS.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110819"},"PeriodicalIF":5.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145678286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of brassinolide in storage root formation under nitrogen–density interaction in sweet potato: Genome-wide identification of IbDET2 and IbBES1/BZR1 gene families","authors":"Xue Hai ,&nbsp;Yanhui Lin ,&nbsp;Hongjuan Liu ,&nbsp;Xin Guo ,&nbsp;Xianming Zhou ,&nbsp;Huixian Xing ,&nbsp;Chengcheng Si","doi":"10.1016/j.plaphy.2025.110833","DOIUrl":"10.1016/j.plaphy.2025.110833","url":null,"abstract":"<div><div>Reasonable management of nitrogen levels (NL) and planting densities (PD) is pivotal for the formation of sweet potato storage roots (SR) and enhancing yield. Brassinolide (BR) plays a regulatory role in root development. However, the characteristics of the <em>DET2</em> and <em>BES1/BZR1</em> gene families, which regulate BR metabolism in sweet potatoes, and their response mechanisms to NL and PD remain unclear. In the 2023–2024 period, this study employed 'Pushu 32′ as the test material to investigate the response of sweet potato yield in field experiments, root development during the SR formation stage (15–45 days) in micro-plot experiments, and BR content to various combinations of four NL levels [0 (NN), 60 (LN), 120 (MN), and 180 (HN) kg N ha⁻¹] and three PD levels [50,000 (LD), 62,500 (MD), and 83,250 (HD) plants ha⁻¹]. The study aimed to identify and analyze <em>IbDET2</em> and <em>IbBES1/BZR1</em>, clarifying their responses to NL and PD, as well as their relationship with SR formation. The results indicated that: (1) Compared with the traditional LDHN combination, MDMN significantly increased sweet potato yield by augmenting the number of SRs per plant and per unit area, with a yield increase ranging from 26.86 % to 32.42 %. (2) MDMN notably elevated BR content during the 15–45 days period and increased the number and total weight of roots. (3) Within the sweet potato genome, 13 <em>IbDET2</em> genes and 11 <em>IbBES1/BZR1</em> genes were identified. Their physicochemical properties, gene and protein structures, phylogenetic relationships, cis-elements, collinear relationships, expression patterns, correlations, homologous genes with sequence alignments, protein interaction networks, and molecular docking were analyzed using bioinformatics tools to elucidate the family information and characteristics. Based on their expression patterns and correlations, it is postulated that <em>IbDET2-8</em> and <em>IbBES1/BZR1-10</em> are key genes responsive to MDMN, potentially enhancing the number of sweet potato SR by regulating BR metabolism, thereby laying the groundwork for increased yield. This study provides preliminary insights into the physiological and molecular mechanisms by which the <em>IbDET2</em> and <em>IbBES1/BZR1</em> families regulate SR formation in sweet potato in response to nitrogen reduction under high planting density.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110833"},"PeriodicalIF":5.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145678345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coordinated ethylene and reactive oxygen species signaling regulates root hair morphogenesis for nitrogen acquisition in Arabidopsis","authors":"Zhen Wang ,&nbsp;Peifan Wang ,&nbsp;Jiarui Xu ,&nbsp;Bidan Yin ,&nbsp;Xin Liang ,&nbsp;Xianyong Lin ,&nbsp;Chengliang Sun","doi":"10.1016/j.plaphy.2025.110845","DOIUrl":"10.1016/j.plaphy.2025.110845","url":null,"abstract":"<div><div>Root hairs are crucial for nutrient acquisition and exhibit high developmental plasticity in response to external nutrient availability. However, the specific root hair responses and mechanisms that integrate external nitrogen (N)-limitation signals into the root hair development remain poorly understood. This study revealed that under N deficiency, a set of genes associated with root hair development and ethylene responses was markedly upregulated in <em>Arabidopsis</em> roots. On a molecular level, the transcriptional activity of ETHYLENE INSENSITIVE 3 (EIN3), a master transcription factor of ethylene signaling, was augmented in the root vascular tissues under N deficiency, as shown by the <em>5×EBS:GUS</em> staining. Further research using the ethylene-insensitive mutant <em>ein3-1eil1-1</em> exhibited significantly reduced root hair length under low-N conditions. The inhibitory effect was further confirmed by ethylene synthesis inhibitor aminoethoxyvinylglycine (AVG). We also found that low nitrate significantly enriched ROS-related metabolic processes. Subsequent functional analyses revealed that the resulted ROS is specifically required for root hair elongation. Crucially, we demonstrate that ROS accumulation is required for the ethylene-dependent growth of root hairs under N deficiency. Taken together, these results suggest that N deficiency triggers a signaling cascade involving both ethylene and ROS to regulate root hair elongation, thereby enhancing the plant's capacity for nutrient acquisition.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"230 ","pages":"Article 110845"},"PeriodicalIF":5.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145690573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A one-bp deletion in BoDW1 encoding microtubule-associated kinase causes dwarfing in Brassica oleracea","authors":"Miaomiao Xing ,&nbsp;Henan Su ,&nbsp;Zhiliang Xiao ,&nbsp;Yong Wang ,&nbsp;Jialei Ji ,&nbsp;Limei Yang ,&nbsp;Yuanyuan Xu ,&nbsp;Yuyu Lu ,&nbsp;Jiyong Yan ,&nbsp;Zhiyuan Fang ,&nbsp;Yangyong Zhang ,&nbsp;Liwang Liu ,&nbsp;Aisong Zeng ,&nbsp;Honghao Lv","doi":"10.1016/j.plaphy.2025.110429","DOIUrl":"10.1016/j.plaphy.2025.110429","url":null,"abstract":"<div><div>Cabbage (<em>Brassica oleracea</em> var. <em>capitata</em>) is an important vegetable crop that is widely cultivated throughout the world. Plant height is a key agronomic trait in cabbage, influencing architecture and yield, and is mainly determined by cell division and stem expansion. Previously, we identified a natural cabbage dwarf mutant, 99-198dw, which displays compact plant architecture, shortened internodes, and small leaves with reduced cell numbers. However, the molecular mechanism underlying this trait remains unknown. In this study, we identified the causal gene <em>Bodw1</em> using a map-based cloning approach. A 1-bp deletion in the eighth exon of <em>BoDW1</em> resulted in the mutant phenotype. Knockdown and knockout of <em>BoDW1</em> in wild-type cabbage lines via RNAi and CRISPR/Cas9 resulted in abnormal phenotypes observed in 99-198dw. <em>BoDW1</em>, homologous to the Arabidopsis gene <em>RUNKEL</em>, encodes a microtubule-associated kinase-like protein localized to microtubules and is mainly expressed in proliferating young tissues. Co-IP-MS, yeast two-hybrid, and BiFC showed that BoDW1 interacts directly with BoCDC48C, a key factor in membrane fusion at the cell division plane. Transcriptome analysis further identified differentially expressed genes associated with membrane fusion and cell plate formation. Our results suggest that <em>BoDW1</em> probably regulates cabbage development by participating in cell division. Together, our study provides new insights into the molecular control of plant height.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110429"},"PeriodicalIF":5.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145006597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a novel O-glycosyltransferase PgGT2 for catalyzing the first glycosylation step of saponin synthesis in Platycodon grandiflorum","authors":"Zhen Li ,&nbsp;Wenjing Yu ,&nbsp;Weiwei Tang,&nbsp;Xu Lu,&nbsp;Bin Li","doi":"10.1016/j.plaphy.2025.110792","DOIUrl":"10.1016/j.plaphy.2025.110792","url":null,"abstract":"<div><div><em>Platycodon grandiflorum,</em> a widely used medicinal and edible plant, is rich in bioactive triterpenoid saponins, whose structural diversity, pharmacological activity, and bioavailability are closely related to glycosylation. However, the enzyme initiating the glycosylation of platycodigenin-type aglycone in <em>P. grandiflorum</em> remained unidentified. Herein, PgGT2 was discovered from <em>P. grandiflorum</em>, a novel UGT73 subfamily member that specifically catalyzes the initial <em>β</em>-D-glycosylation at the C-3 hydroxyl group of platycodigenin and produces the key intermediate 3-<em>O</em>-<em>β</em>-D-glucopyranosylplatycodigenin. Phylogenetic analysis revealed that PgGT2 was classified into the UGT73 subfamily associated with C-3 glycosylation of triterpenoid. <em>In vitro</em> assays demonstrated its strict substrate and sugar donor selectivity. Transient expression in <em>Nicotiana benthamiana</em> was performed to confirm its transglycosylation activity <em>in</em> <em>vivo</em>. Molecular docking, molecular dynamics simulations, and site-directed mutagenesis were performed to better understand the potential catalytic mechanism of PgGT2. The residues His22, Trp369, Asn370, and Gln391 played important roles in stabilizing UDP-glucose as the sugar donor, and Arg93 was critical for the stabilization of platycodigenin as the substrate. This study clarified the initial glycosylation of sapogenin in <em>P. grandiflorum</em>, a key step in the biosynthesis of triterpenoid saponins. Also, it could provide a pivotal enzyme for engineering saponin production.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110792"},"PeriodicalIF":5.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold resistance identification and transcriptomic responses of rapeseed (Brassica napus L.) seedlings under cold stress","authors":"Xiaoling Zhang ,&nbsp;Qinli Yang ,&nbsp;Hongli Li ,&nbsp;Lixian Zhang ,&nbsp;Caihong Sun ,&nbsp;Huanyang Zhang ,&nbsp;Huanli Li ,&nbsp;Jing Li ,&nbsp;Yonghong Zhu ,&nbsp;Chunfang Du ,&nbsp;Xiaoxia Shangguan","doi":"10.1016/j.plaphy.2025.110778","DOIUrl":"10.1016/j.plaphy.2025.110778","url":null,"abstract":"<div><div>Freezing temperatures during winter and abrupt temperature fluctuations in winter and early spring pose significant threats to <em>Brassica napus</em> survival, often resulting in substantial yield losses. In this study, three rapeseed lines with close genetic relationships demonstrating notable differences in cold tolerance, were selected for transcriptome analysis, with SX9 exhibiting superior cold resistance compared to SX7 and SX8. RNA-seq revealed numerous differentially expressed genes (DEGs) associated with cold response across these lines. Specifically, 2390 common DEGs were found in SX7, SX8, and SX9, potentially involved in broad cold stress responses, while 3707 DEGs unique to SX9 may contribute to its enhanced cold tolerance. Functional annotation of upregulated genes indicated involvement in processes such as response to abiotic stimuli, oxygen-containing compounds, and the MAPK signaling pathway, while downregulated genes primarily associated with photosynthesis. Transcription factor (TF) analysis identified 3889 TFs within these DEGs, including basic helix-loop-helix (bHLH), MYB, NAC, ethylene-responsive factor (ERF), and WRKY, encompassing key cold-responsive regulatory factors such as SCREAM/inducer of CBF expression 1 (SCRM/ICE1), NAC29, NAC56, dehydration-responsive element-binding protein 1B (DREB1B), and ERF70. Based on gene expression profiles and functional annotations, 139 DEGs were predicted to play critical roles in cold stress responses in rapeseed seedlings. Protein interaction network analysis of them suggested that rapeseed seedlings respond to low temperatures <em>via</em> the DREB1B-dependent pathways, in coordination with other biological processes. Additionally, the candidate gene <em>BnERF70</em>, an AP2/ERF family member upregulated by cold stress, was shown to enhance cold resistance in transgenic <em>Arabidopsis thaliana</em> when overexpressed. This study provides valuable insights into the molecular mechanisms of cold responsiveness in rapeseed seedlings.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110778"},"PeriodicalIF":5.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Absorption, transport, and bioavailability of lead (Pb) in plant systems and nanomaterial detoxification mechanisms","authors":"Lingling Jin ,&nbsp;Qian Qiao ,&nbsp;Ying Xiong ,&nbsp;Zhongqing Liu ,&nbsp;Yi Wang ,&nbsp;Ensen Guan ,&nbsp;Peng Zhang ,&nbsp;Zhao Chen ,&nbsp;Li Zhang ,&nbsp;Xin Hou ,&nbsp;Long Yang ,&nbsp;Yun Gao","doi":"10.1016/j.plaphy.2025.110785","DOIUrl":"10.1016/j.plaphy.2025.110785","url":null,"abstract":"<div><div>Lead (Pb) is a highly toxic pollutant, and with increases in industrial and agricultural activities, the problems related to soil Pb pollution are becoming increasingly prominent and pose serious threats to food production and human health. Pb toxicity severely inhibits plant growth, specifically manifested as impaired photosynthesis, increased oxidative stress, and metabolic disorders. Nanoremediation refers to using nanotechnology-based materials and processes to remediate environmental contaminants, and nanotechnology offers innovative solutions for the remediation of Pb contamination. Therefore, understanding the behavior of Pb in plants and the mechanisms through which Pb interacts with nanomaterials is important for formulating effective nanomaterial remediation strategies and ensuring the safety of agricultural products. This article provides a comprehensive review of the absorption, transport, and bioavailability of Pb in plant systems, as well as the roles and mechanisms of nanomaterials in reducing Pb accumulation, increasing plant photosynthetic parameters and antioxidant capacity, regulating gene expressions and influencing physiological metabolism in plants. This study provides comprehensive information for understanding the behavior of Pb in plant systems and its interaction with nanomaterials and serves as a valuable reference for future research and the formulation of remediation strategies based on nanomaterials.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"229 ","pages":"Article 110785"},"PeriodicalIF":5.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}