Plant Physiology and Biochemistry最新文献_第5页

The AeMYBs responding to CMCS cooperatively regulate the phenylpropanoid pathway to participate in postharvest lignin biosynthesis of okra 响应CMCS的AeMYBs协同调节苯丙素途径，参与秋葵采后木质素的生物合成。

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-12-29 DOI: 10.1016/j.plaphy.2025.111000

Lijuan Wei , Yujie Shi , Shunjiao Gao , Huanqi Wu , Shang Lin , Shuxiang Liu , Wen Qin

Lignification is one of the key factors restricting the postharvest storage quality of okra. Analyzing the key factors inducing lignin deposition is beneficial for targeted regulation of the quality deterioration caused by postharvest lignification of okra. The aim of this study is to elucidate the molecular mechanisms of key v-myb avian myeloblastosis viral oncogene homolog (MYB) transcription factors involved in postharvest lignification of okra in response to carboxymethyl chitosan (CMCS). Subcellular localization experiments confirmed that AeMYB6, AeMYB315-1, and AeMYB35-2 are all nuclear localization proteins. The dual luciferase reporter gene (DLR) and yeast one hybrid (Y1H) experiments showed that the three AeMYBs can directly bind to promoters of multiple structural genes in the phenylpropanoid pathway, such as AePAL1/2/3/4 and AePOD1/2/3/4. Further transient overexpression (OE) experiments revealed that AeMYB6, AeMYB315-1, and AeMYB35-2 upregulated the expression of multiple genes (AePOD, Ae4CL, AeCAD, and AePAL) and enzyme activity in the phenylpropanoid pathway, including phenylalanine ammonia lyase (PAL), peroxidase (POD), 4-coumarin-CoA ligase (4CL), and cinnamoyl dehydrogenase (CAD). Metabolite analysis showed that lignin-related metabolites were reduced to varying degrees, such as coniferyl alcohol, sinapic acid, 4-hydroxycinnamic acid, caffeic acid, L-phenylalanine and ferulic acid. In summary, this study found that under the regulation of CMCS, AeMYB6, AeMYB315-1, and AeMYB35-2 cooperatively regulate the expression of multiple genes in the phenylpropanoid pathway, jointly regulating lignin accumulation. This result provides important scientific basis for revealing the mechanism of CMCS delaying postharvest lignification of okra.

木质素化是制约秋葵采后贮藏品质的关键因素之一。分析诱发木质素沉积的关键因素，有助于有针对性地调控秋葵采后木质素化导致的品质劣化。本研究的目的是阐明关键v-myb禽成髓细胞病病毒致癌基因同源（MYB）转录因子参与秋葵采后木质化响应羧甲基壳聚糖（CMCS）的分子机制。亚细胞定位实验证实AeMYB6、AeMYB315-1、AeMYB35-2均为核定位蛋白。双荧光素酶报告基因（DLR）和酵母单杂交（Y1H）实验表明，这3种AeMYBs可以直接结合苯丙素途径中多个结构基因的启动子，如AePAL1/2/3/4和AePOD1/2/3/4。进一步的瞬时过表达（OE）实验表明，AeMYB6、AeMYB315-1和AeMYB35-2上调了苯丙氨酸途径中多个基因（AePOD、Ae4CL、AeCAD和AePAL）的表达和酶活性，包括苯丙氨酸解氨酶（PAL）、过氧化物酶（POD）、4-香豆素-辅酶a连接酶（4CL）和肉桂酰脱氢酶（CAD）。代谢物分析表明，木质素相关代谢物如松柏醇、辛酸、4-羟基肉桂酸、咖啡酸、l -苯丙氨酸和阿威酸等均有不同程度的减少。综上所述，本研究发现，在CMCS的调控下，AeMYB6、AeMYB315-1、AeMYB35-2协同调控苯丙素途径中多个基因的表达，共同调控木质素积累。该结果为揭示CMCS延缓秋葵采后木质化的机理提供了重要的科学依据。

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引用次数: 0

Intragenic overexpression of TONNEAU 1b enhances grain length and weight in durum wheat TONNEAU 1b基因的基因内过表达增加了硬粒小麦的粒长和粒重。

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-12-28 DOI: 10.1016/j.plaphy.2025.110998

Chiara D'Attilia , Francesco Camerlengo , Samuela Palombieri , Arianna Frittelli , Csaba Éva , Francesco Sestili

Grain yield is a complex trait determined by multiple genes, physiological and developmental processes, including those governed by cytoskeletal dynamics. In this study, we investigated the role of the TONNEAU 1b (TON1b) gene, a core component of the TON1–TRM–PP2A (TTP) complex involved in microtubule organization and cell division plane positioning, in the polyploid context of durum wheat (Triticum turgidum ssp. durum). To explore its function and agronomic potential, we developed intragenic Svevo lines overexpressing TON1b under the control of the endosperm-specific 1Dx5 promoter, using a CRE-LOX recombination system to produce marker-free genotypes. Molecular and phenotypic analyses of fixed intragenic lines revealed significant increases in grain length, grain weight, spikelet number and total starch content, without compromising grain number per spike. Transcriptomic profiling indicated consistent TON1b overexpression alongside downregulation of CEN1 and TON2, and upregulation of GW7, suggesting a regulatory role in both cell division and elongation processes. These findings suggest a role of TON1b on cytoskeletal remodelling and grain development, offering a promising genetic lever for yield improvement in durum wheat.

粮食产量是一个复杂的性状，由多种基因、生理和发育过程决定，其中包括细胞骨架动力学。在这项研究中，我们研究了TON1-TRM-PP2A （TTP）复合体的核心成分TON1b （TON1b）基因在硬粒小麦（Triticum turgidum ssp）多倍体中参与微管组织和细胞分裂平面定位的作用。硬质)。为了探索TON1b的功能和农艺潜力，我们在胚乳特异性1Dx5启动子的控制下，利用CRE-LOX重组系统，培养了过表达TON1b的Svevo基因内系。分子和表型分析表明，固定基因内系的粒长、粒重、小粒数和总淀粉含量显著增加，但每穗粒数不受影响。转录组学分析显示，TON1b一致过表达，同时CEN1和TON2下调，GW7上调，表明其在细胞分裂和伸长过程中都有调节作用。这些发现提示TON1b在细胞骨架重塑和籽粒发育中的作用，为提高硬粒小麦产量提供了一个有希望的遗传杠杆。

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引用次数: 0

Dynamics of DNA methylation and histone acetylation and methylation during European chestnut bud development 板栗芽发育过程中DNA甲基化、组蛋白乙酰化和甲基化的动态变化

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-12-27 DOI: 10.1016/j.plaphy.2025.110997

Sofia Alves , Ana Teresa Alhinho , Rómulo Sobral , Maria Manuela Ribeiro Costa , Leonor Morais-Cecílio

In perennial plants, tight regulation of bud development, from bud set, through dormancy, to bud burst, is critical for survival and is known to be regulated by epigenetic mechanisms. In Castanea sativa, a major temperate forest tree, the contribution of epigenetic regulators to bud development is poorly understood. To address this, we carried out an integrated study of bud morphology, immunodetection of epigenetic marks and gene expression. We analysed the distribution of two antagonistic epigenetic marks in the shoot apical meristem (SAM) by immunolocalizing 5-methylcytosine (5 mC) and histone H3 acetylated at Lysine 18 (H3K18ac), markers commonly associated with repressive and active chromatin states, respectively. Both marks displayed different distribution patterns in the SAM. In reproductive buds, distal SAM layers (L1, L2 and L3) showed increased 5 mC levels from bud set to dormancy, which then remained constant until burst, whereas H3K18ac levels were higher during bud set and dormancy but decreased at bud burst. To elucidate the influence of several chromatin modifiers in bud developmental stages, we profiled their expression throughout bud development. DNA chromomethylases (CsaCMT2 and CsaCMT3), the chromatin remodeler CsaSWC4 (SWR1 COMPLEX 4), the histone demethylase JUMONJI 21 (CsaJMJ21), CsaROS1 (REPRESSOR OF SILENCING 1) were highly expressed during active growth. The expression of all of these genes showed a significant positive correlation especially with maximum temperature, and several histone acetylases exhibited an opposite trend.

Overall, this work indicates that small adjustments in DNA methylation levels, which are mediated by the coordinated expression of DNA methyltransferases and demethylases, alongside the dynamics of histone H3 acetylation, appear to play a role in the developmental processes of buds in C. sativa.

在多年生植物中，芽发育的严格调控，从芽定，到休眠，到芽萌发，对生存至关重要，并且已知受表观遗传机制的调控。在温带主要森林树种甜槠中，表观遗传调控因子对芽发育的作用尚不清楚。为了解决这个问题，我们对芽形态、表观遗传标记的免疫检测和基因表达进行了综合研究。我们通过免疫定位5-甲基胞嘧啶（5mc）和赖氨酸18乙酰化组蛋白H3 (H3K18ac)，分析了两个拮抗表观遗传标记在茎尖分生组织（SAM）中的分布，这两个标记通常分别与抑制和活性染色质状态相关。两种标记在SAM中表现出不同的分布模式。在生殖芽中，远端SAM层（L1、L2和L3）的5 - mC水平在芽定植至休眠期间呈上升趋势，并保持不变直至芽破裂，而H3K18ac水平在芽定植和休眠期间较高，在芽破裂时下降。为了阐明几种染色质修饰因子在芽发育阶段的影响，我们分析了它们在芽发育过程中的表达。DNA染色体甲基化酶（CsaCMT2和CsaCMT3）、染色质重塑酶CsaSWC4 （SWR1 COMPLEX 4）、组蛋白去甲基化酶JUMONJI 21 （CsaJMJ21）、CsaROS1（沉默抑制因子1）在活性生长期间高表达。这些基因的表达量与最高温度呈显著正相关，而组蛋白乙酰化酶的表达量与最高温度呈显著正相关。总的来说，这项工作表明，DNA甲基化水平的微小调整，由DNA甲基转移酶和去甲基化酶的协调表达介导，以及组蛋白H3乙酰化的动态，似乎在苜蓿芽的发育过程中发挥作用。

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引用次数: 0

Time dependent heat stress responses in rice and the role of brassinosteroids in regulating thermotolerance 水稻的时间依赖性热应激反应和油菜素内酯在调节耐热性中的作用

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-12-26 DOI: 10.1016/j.plaphy.2025.110996

Zoya Batool Naqvi , Yanxi Liu , Wenjing Meng , Wenchao Yin , Nana Dong , Xiaoliang Wang , Xicheng Li , Yanzhao Yang , Jihong Liu , Honglei Ma , Simeng Geng , Qian Qian , Hongning Tong , Mei Niu

Understanding the dynamic responses of rice (Oryza sativa L.) to varying durations of heat stress is crucial for developing thermotolerant crops in the face of global climate trends. Here, we evaluated the responses of rice cultivar Zhonghua11 to short-term (0.5–1 h) and long-term (12–24 h) heat stress at 45 °C. Phenotypic observations showed that short-term heat stress reduced photosynthetic pigment content without major leaf morphological changes, while long-term stress caused severe leaf drying and reduced the survival rate to 13 %. Transcriptome analysis revealed persistent inhibition of chlorophyll metabolism and photosynthesis genes under heat stress. Short-term heat stress primarily induced genes involved in reactive oxygen species scavenging and heat shock proteins, while long-term stress shifted the transcriptional profile toward DNA damage repair and chloroplast reconstruction, indicating the dynamic molecular nature of rice's response. Furthermore, set of BR-linked genes were identified from transcriptome analysis and exogenous BR application was confirmed to improve plants survival rate. Functional analyses of BR-linked genes (OsBZR1, OsBU1, and OsGSR1) confirmed that BR signaling enhances thermotolerance. Based on these results, we conclude that rice employs distinct dynamic strategies to cope with heat stress of different durations. Importantly, this study clarifies rice's dynamic strategies under heat stress and highlights BR pathways as key regulators, providing a basis for breeding heat-resilient varieties.

了解水稻（Oryza sativa L.）对不同持续时间热胁迫的动态响应对于在全球气候趋势下培育耐热作物至关重要。在45°C条件下，研究了中花11号对短期（0.5-1 h）和长期（12-24 h）热胁迫的响应。表型观察表明，短期热胁迫降低了光合色素含量，但叶片形态无明显变化，而长期热胁迫导致叶片严重干燥，使成活率降低至13%。转录组分析显示，高温胁迫下叶绿素代谢和光合作用基因持续受到抑制。短期热胁迫主要诱导参与活性氧清除和热休克蛋白的基因，而长期热胁迫将转录谱转向DNA损伤修复和叶绿体重建，表明水稻响应的动态分子性质。此外，通过转录组分析鉴定出一组BR连锁基因，并证实外源BR应用可提高植株成活率。BR相关基因（OsBZR1， OsBU1和OsGSR1）的功能分析证实BR信号增强了耐热性。基于这些结果，我们得出结论，水稻采用不同的动态策略来应对不同持续时间的热胁迫。重要的是，本研究阐明了水稻在热胁迫下的动态策略，并强调了BR通路作为关键调控因子，为耐热品种的选育提供了依据。

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引用次数: 0

Genome-wide analysis of LSH genes reveals evolutionary patterns in Rosaceae and a positive role for MdLSH10b in adventitious root formation LSH基因的全基因组分析揭示了蔷薇科植物的进化模式，以及MdLSH10b在不定根形成中的积极作用。

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-12-26 DOI: 10.1016/j.plaphy.2025.110983

Xi Li , Shuyang Gu , Donglin Huang , Liu Cong , Yawen Shen , Yujie Zhao , Wanyu Xu , Pengbo Hao , Kunxi Zhang , Miaomiao Wang , Jian Jiao , Chunhui Song , Ran Wan , Jiangli Shi , Tuanhui Bai , Shangwei Song , Yu Liu , Jiancan Feng , Xianbo Zheng

The plant-specific LIGHT-SENSITIVE SHORT HYPOCOTYL (LSH) genes are integral to the development of plant organs. However, the evolution and function of LSH genes in Rosaceae remain poorly understood. Here, 60 LSH genes were systematically identified across the genomes of six Rosaceae species. Phylogenetic analysis grouped these LSH genes into four distinct clades. The analysis of LSH gene family duplications revealed that whole-genome duplication (WGD) and transposed duplication (TRD) primarily drive the expansion of the LSH gene family in Rosaceae species. In apple, 18 MdLSH gene pairs generated through WGD have undergone purifying selection. Further transcriptome analysis demonstrated that MdLSH genes exhibit distinct expression patterns across different tissues. Among them, MdLSH10b was significantly induced at the induction stage of adventitious roots. The integration of transcriptome and promoter analyses indicated that MdLSH10b potentially regulates the expression of MdWOX4a. Yeast one-hybrid assays (Y1H) and β-glucuronidase (GUS) activity analyses demonstrated that MdLSH10b transcriptionally activates MdWOX4a. Notably, transient overexpression of MdLSH10b in apple resulted in an increased rate of adventitious root induction. In conclusion, this study suggests that MdLSH10b may promote adventitious root formation by transcriptionally activating MdWOX4a and provides new genetic resources for further functional investigations on MdLSH genes in apple development.

植物特有的光敏感短下胚轴（LSH）基因是植物器官发育的重要组成部分。然而，对蔷薇科LSH基因的进化和功能仍知之甚少。本文系统地鉴定了6个蔷薇科物种基因组中的60个LSH基因。系统发育分析将这些LSH基因分为四个不同的分支。LSH基因家族重复分析表明，全基因组重复（WGD）和转座重复（TRD）是蔷薇科物种LSH基因家族扩增的主要驱动因素。在苹果中，通过WGD产生的18对MdLSH基因进行了纯化选择。进一步的转录组分析表明，MdLSH基因在不同组织中表现出不同的表达模式。其中，MdLSH10b在不定根诱导阶段被显著诱导。转录组和启动子分析的整合表明，MdLSH10b可能调控MdWOX4a的表达。酵母单杂交实验（Y1H）和β-葡萄糖醛酸酶（GUS）活性分析表明，MdLSH10b转录激活MdWOX4a。值得注意的是，苹果中MdLSH10b的瞬时过表达导致不定根诱导率增加。综上所述，本研究提示MdLSH10b可能通过转录激活MdWOX4a促进不定根的形成，为进一步研究MdLSH基因在苹果发育中的功能提供了新的遗传资源。

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引用次数: 0

Maize CONSTANS-like gene ZmCOL13 promotes flowering time under short-day conditions in Arabidopsis 玉米constans样基因ZmCOL13促进拟南芥在短日照条件下的开花时间

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-12-26 DOI: 10.1016/j.plaphy.2025.110994

Yang Wang , Shuyuan Li , Jiahui Li , Ying Liu , Qingyou Gong , Yanqiang Gao , Jun Huang

Although CONSTANS-like (COL) genes are essential for photoperiodic flowering in plants, their functional diversification in maize (Zea mays) remains unclear. A systematic analysis of the COL family demonstrated that ZmCOL13 exhibits peak expression in mature leaves. Cis-element analysis of its promoter predicted regulation by light, hormones, and stress cues. ZmCOL13 was found to be phylogenetically closely related to AtCOL3-5, OsCOL4, and ZmCOL3. ZmCOL13 encodes a 364-amino acid protein that contains BBOX, B-box1-BBX-like, and CCT domains and was localized to the nucleus, which is consistent with its identity as a plant transcription factor. Heterologous overexpression of ZmCOL13 in Arabidopsis resulted in accelerated flowering by 12–13 days under short-day conditions but showed no significant difference under long-day conditions compared to the wild type (Col-0). Transcriptome analysis identified 1313 differentially expressed genes, and Gene Ontology analysis revealed that two biological process terms associated with photoperiod were significantly enriched. Overexpression of ZmCOL13 increased AtFT expression by several hundred-fold, which may represent a primary mechanism underlying ZmCOL13-induced increases in flowering in Arabidopsis. Based on co-expression and phylogenetic analyses in maize, we developed a model in which ZmCOL13 was predicted to modulate flowering time by partially regulating the expression of Arabidopsis flowering gene AtFT orthologs (ZmZCN7, ZmZCN8, and ZmZCN12).

尽管CONSTANS-like （COL）基因对植物的光周期开花至关重要，但它们在玉米（Zea mays）中的功能多样化尚不清楚。对COL家族的系统分析表明，ZmCOL13在成熟叶片中表达高峰。其启动子的顺式元件分析预测了光、激素和应激线索的调节。ZmCOL13与AtCOL3-5、OsCOL4和ZmCOL3亲缘关系密切。ZmCOL13编码一个364个氨基酸的蛋白，包含BBOX、b -box1- bbx样结构域和CCT结构域，并且定位于细胞核，这与其作为植物转录因子的身份一致。ZmCOL13在拟南芥中异源过表达，在短日照条件下可使开花提前12-13天，而在长日照条件下与野生型（Col-0）相比差异不显著。转录组分析鉴定出1313个差异表达基因，基因本体分析显示，与光周期相关的两个生物过程术语显著丰富。过表达ZmCOL13可使AtFT的表达增加数百倍，这可能是ZmCOL13诱导拟南芥开花增加的主要机制。基于玉米的共表达和系统发育分析，我们建立了一个模型，预测ZmCOL13通过部分调节拟南芥开花基因AtFT同源基因（ZmZCN7、ZmZCN8和ZmZCN12）的表达来调节开花时间。

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引用次数: 0

Crosstalk between physiological and genetic mechanisms during maintenance of acquired thermotolerance in tomato 番茄获得性耐热性维持过程中生理与遗传机制的串扰

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-12-26 DOI: 10.1016/j.plaphy.2025.110995

Yi Liu , Pinke Lv , Yang Sun , Hong Chen , Fei Ding , Fangling Jiang , Xiaqing Yu , Carl-Otto Ottosen , Xiaoming Song , Ron Mittler , Zhen Wu , Rong Zhou

Climate resilient crops are urgently needed to mitigate food insecurity arising from climate change and increased population. Enhancing plant stress memory is an efficient approach for improving crop resilience. We studied the underlying physiological and molecular mechanisms that accompany heat stress memory in tomato (Solanum lycopersicum L.). Compared to non-primed plants, heat primed plants exhibited improved morphological traits, leaf photosynthetic capacity, water utilization efficiency and water potential at 45 °C. Primed plants displayed a transient enhancement in reactive oxygen species (ROS) production together with higher antioxidant enzyme activity, leading to improved ROS homeostasis and reduced oxidative damage during prolonged heat stress. Increased area of stomatal pores, and higher chloroplast ultrastructure stability, were observed in primed plants, compared to non-primed plants at 45 °C. A total of 2049 and 882 differentially expressed genes (DEGs) were specifically identified in primed vs non-primed plants 6- and 12-h post stress application, respectively. These were associated with RNA degradation, ubiquitin mediated proteolysis, mRNA surveillance, glycerophospholipid metabolism and phosphatidylinositol signaling. These physiological and molecular mechanisms likely contributed to tomato resilience during heat stress. Acquired thermotolerance in tomatoes could therefore be mediated by enhanced ROS homeostasis and stomatal regulation, as well as higher structural stability of chloroplasts. Our study sheds new light on the physiological and molecular mechanisms underlying heat stress memory in tomato and provides valuable integrative transcriptomic-phenotypic datasets for this process.

为了缓解气候变化和人口增加造成的粮食不安全，迫切需要具有气候适应能力的作物。增强植物的逆境记忆是提高作物抗逆性的有效途径。研究了番茄（Solanum lycopersicum L.）热应激记忆的生理和分子机制。与未处理植物相比，热处理植物在45°C时表现出更好的形态特征、叶片光合能力、水分利用效率和水势。在长时间的热胁迫下，植物的活性氧（ROS）生成瞬间增强，抗氧化酶活性提高，从而改善了活性氧稳态，减少了氧化损伤。45℃下，与未处理的植株相比，处理过的植株气孔面积增加，叶绿体超微结构稳定性提高。在胁迫后6和12 h，分别鉴定出2049个和882个差异表达基因（deg）。这些与RNA降解、泛素介导的蛋白质水解、mRNA监测、甘油磷脂代谢和磷脂酰肌醇信号传导有关。这些生理和分子机制可能有助于番茄在高温胁迫下的恢复力。因此，番茄获得性耐热性可能是通过增强活性氧稳态和气孔调节，以及叶绿体结构的更高稳定性来介导的。我们的研究揭示了番茄热胁迫记忆的生理和分子机制，并为这一过程提供了有价值的综合转录组-表型数据集。

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引用次数: 0

Arbuscular mycorrhizal fungi enhance drought resilience and bioactive compound accumulation in Polygonum cuspidatum 丛枝菌根真菌增强虎杖的抗旱性和生物活性物质积累。

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-12-25 DOI: 10.1016/j.plaphy.2025.110973

Zeng-Zheng Zhang , Hai-Dong Feng , Ze-Zhi Zhang , Yue Wen , Abeer Hashem , Qiang-Sheng Wu

Polygonum cuspidatum, a key source of resveratrol and polydatin, faces cultivation challenges due to drought, which impairs plant growth and bioactive compound synthesis. This study investigated the effects of inoculating P. cuspidatum with arbuscular mycorrhizal fungus Funneliformis mosseae on its drought resilience and bioactive compound accumulation under drought stress. Although drought significantly reduced mycorrhizal colonization rates, inoculation with F. mosseae under drought still significantly enhanced aerial growth (plant height, stem diameter, leaf number, and aboveground biomass), phosphorus (P) levels, nitrogen balance index, and root development (total length, projected area, surface area, and volume). Drought stress profoundly elevated leaf superoxide dismutase, peroxidase (POD), and catalase (CAT) activities and proline levels, while F. mosseae further amplified these enzyme activities, particularly boosting POD and CAT under well-watered conditions and all three antioxidant enzymes under drought conditions, followed by a substantial decrease in proline levels. Drought diminished the levels of four active components (polydatin, resveratrol, aloe-emodin, and physcion), while F. mosseae differentially enhanced the levels of specific medicinal components, as evidenced by an increase in polydatin, resveratrol, aloe-emodin, and physcion under well-watered and an elevation in polydatin, resveratrol, aloe-emodin, and chrysophanol under drought. Gene expression analysis revealed that drought triggerred the down-regulated expression of resveratrol biosynthesis genes, while F. mosseae upregulated all six tested genes under well-watered conditions and selectively enhanced PcCHS1, PcCHS12, PcRS11, and PcCRS1 under drought conditions. This emphasizes that arbuscular mycorrhizal fungal inoculation is a promising strategy to enhance plant establishment and bioactive compound accumulation of P. cuspidatum in drought-prone environments.

虎杖是白藜芦醇和白藜芦醇的重要来源，由于干旱影响了植物的生长和生物活性化合物的合成，虎杖的种植面临着挑战。研究了在干旱胁迫下，用丛枝菌根真菌莫塞漏斗菌（funeliformis mosseae）接种虎杖对其抗旱性和活性物质积累的影响。尽管干旱显著降低了菌根定植率，但干旱条件下接种mosseae仍显著提高了地上生长（株高、茎粗、叶数和地上生物量）、磷(P)水平、氮平衡指数和根系发育（总长度、投影面积、表面积和体积）。干旱胁迫显著提高了叶片超氧化物歧化酶、过氧化物酶（POD）和过氧化氢酶（CAT）的活性和脯氨酸水平，而F. mosseae进一步提高了这些酶的活性，特别是在水分充足的条件下提高了POD和CAT的活性，在干旱条件下提高了所有三种抗氧化酶的活性，随后脯氨酸水平大幅下降。干旱降低了四种有效成分（聚大黄素、白藜芦醇、芦荟大黄素和白藜芦醇）的水平，而F. mosseae则不同程度地提高了特定药用成分的水平，这可以从水分充足的情况下聚大黄素、白藜芦醇、芦荟大黄素和白藜芦醇的增加和干旱情况下聚大黄素、白藜芦醇、芦荟大黄素和大黄酚的升高中得到证明。基因表达分析表明，干旱导致白藜芦醇生物合成基因表达下调，而F. mosseae在水分充足的条件下上调了所有6个基因的表达，并选择性地增强了PcCHS1、PcCHS12、PcRS11和PcCRS1的表达。这说明在干旱环境下，丛枝菌根真菌接种是一种很有前途的策略，可以促进虎杖的成树和生物活性化合物的积累。

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引用次数: 0

Genome-wide identification of the VQ gene family from Limonium bicolor and functional characterization of LbVQ6 under salt stress 盐胁迫下双色柠檬VQ基因家族的全基因组鉴定及LbVQ6的功能表征

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-12-24 DOI: 10.1016/j.plaphy.2025.110978

Ruyi Yang , Zonghui Zhang , Aijuan Jiang , Zhihui Zhu , Xiaofang Chen , Hualing Xu , Kushiev Khabibjon H , Jianrong Guo , Min Chen

VQ motif-containing proteins are recognized as key regulators involved in plant growth, development, and responses to environmental stresses. Despite their established roles in various plant species, this gene family remains insufficiently characterized in Limonium bicolor, a recretohalophytic species notable for its multicellular salt glands. In the present study, 6 LbVQ genes were systematically identified from the L. bicolor genome and classified into four phylogenetic groups. Analyses of gene structure and conserved motifs revealed a high degree of conservation among LbVQ family members. Chromosomal mapping indicated an uneven distribution of these genes across 4 chromosomes. Promoter region analysis uncovered numerous cis-acting elements related to hormonal and stress-responsive pathways. Analysis of expression patterns demonstrated that the majority of LbVQ genes exhibited elevated expression during early salt gland development, suggesting their involvement in salt secretion mechanisms. Among them, LbVQ6 displayed significant differential expression under salt treatment. Functional validation through transient gene silencing experiments indicated that LbVQ6 positively regulate the development of salt glands and the salt tolerance of this species. Collectively, these findings establish a foundation for understanding the regulatory roles of VQ genes in halophytes, particularly in salt stress adaptation.

含有VQ基序的蛋白被认为是参与植物生长、发育和对环境胁迫反应的关键调节因子。尽管这个基因家族在许多植物物种中都有明确的作用，但在以多细胞盐腺而闻名的双色Limonium bicolor中，这个基因家族的特征仍然不够充分。本研究从双色l基因组中系统鉴定了6个LbVQ基因，并将其划分为4个系统发育群。基因结构和保守基序分析显示LbVQ家族成员之间具有高度的保守性。染色体定位表明这些基因在4条染色体上分布不均匀。启动子区域分析揭示了许多与激素和应激反应途径相关的顺式作用元件。表达模式分析表明，大多数LbVQ基因在盐腺发育早期表达升高，提示其参与盐分泌机制。其中，LbVQ6在盐处理下表现出显著差异表达。通过瞬时基因沉默实验功能验证表明，LbVQ6正调控盐腺发育和盐耐受性。总的来说，这些发现为理解VQ基因在盐生植物，特别是在盐胁迫适应中的调节作用奠定了基础。

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引用次数: 0

The BES1/BZR1 transcriptional factor SlBES2 cross talks with abscisic acid to regulate fruit maturation in tomato BES1/BZR1转录因子SlBES2与脱落酸互交调控番茄果实成熟。

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-12-24 DOI: 10.1016/j.plaphy.2025.110984

Ming Huang , Wenjing Liu

Abscisic acid plays a pivotal role in initiating fruit maturation, yet the underlying mechanism is still far from being clearly revealed. This study identified a tomato BES1/BZR1 transcriptional factor SlBES2 as a regulator of fruit maturation through comparative observation of SlBES2-overexpressing and SlBES2-repressing tomato lines. The peaks of fruit abscisic acid content were advanced in SlBES2-overexpressing lines but delayed in SlBES2-repressing lines. And the variations of fruit abscisic acid content were negatively correlated with the gene expression patterns of SlBES2 during fruit maturation. Comparative transcriptome and gene expression analysis revealed that SlBES2 regulates expression levels of genes involved in abscisic acid biosynthesis and catabolism pathway. In turn, both the gene expression level and unphosphorylated protein abundance of SlBES2 are negatively regulated by abscisic acid with the approach of fruit maturation. In addition, SlBES2 physically interacts with type 2C protein phosphatases and the interaction could be inhibited by abscisic acid, indicating that type 2C protein phosphatases might mediate the crosstalk between SlBES2 and abscisic acid signaling pathway. Together, this study demonstrates that SlBES2 cross talks with abscisic acid during fruit maturation, coordinatively regulating fruit maturation and synchronism of maturity. Our findings contribute a new actor to the complex mechanisms underlying fruit maturation and may provide insights for potential breeding strategies aimed at improving synchronism of fruit maturity.

脱落酸在启动果实成熟中起着关键作用，但其潜在机制尚不清楚。本研究通过比较观察过表达SlBES2和抑制SlBES2的番茄品系，确定了番茄BES1/BZR1转录因子SlBES2对果实成熟的调控作用。slbes2过表达系果实脱落酸含量峰值提前，slbes2抑制系果实脱落酸含量峰值延迟。果实脱落酸含量的变化与果实成熟过程中SlBES2基因的表达模式呈负相关。比较转录组和基因表达分析显示，SlBES2调控脱落酸生物合成和分解代谢途径相关基因的表达水平。随着果实成熟的临近，脱落酸对SlBES2的基因表达水平和未磷酸化蛋白丰度进行负调控。此外，SlBES2可与2C型蛋白磷酸酶发生物理相互作用，且这种相互作用可被脱落酸抑制，说明2C型蛋白磷酸酶可能介导了SlBES2与脱落酸信号通路之间的串扰。综上所述，本研究表明，SlBES2在果实成熟过程中与脱落酸进行交叉对话，协调调节果实成熟和成熟同步性。我们的发现为揭示果实成熟的复杂机制提供了新的线索，并可能为提高果实成熟同步性的潜在育种策略提供见解。

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引用次数: 0