Plant Physiology and Biochemistry最新文献

{"title":"Integrated transcriptomic and metabolomic analyses reveal hormone-mediated crosstalk during potato virus Y and potato spindle tuber viroid co-infection","authors":"Wajahat Hussain ,&nbsp;Meijia Wu ,&nbsp;Yicong Wu ,&nbsp;Guangyan Li ,&nbsp;Dianqiu Lv ,&nbsp;Yonghong Zhou","doi":"10.1016/j.plaphy.2026.111117","DOIUrl":"10.1016/j.plaphy.2026.111117","url":null,"abstract":"<div><div>Plants often face multiple pathogen attacks at once, but how they handle these complex infections at the molecular level is not well understood. This study uses transcriptomic and metabolomic analyses to explore the interaction between potato virus Y (PVY), a protein-coding RNA virus, and potato spindle tuber viroid (PSTVd), a non-coding RNA pathogen, which could infect potato plants together. Samples from different potato fields showed a strong prevalence of PVY, whereas PSTVd exhibited low occurrence compared to PVY but frequently co-occurred with it, indicating potential interactions between these pathogens. Observations of plant symptoms confirmed a PVY dominant role, causing severe stunting and yellowing, while PSTVd milder effects were hidden in co-infected plants. Transcriptomic data revealed PVY broadly alters plant processes like photosynthesis, carbon use, and immune responses, while PSTVd targets specific pathways, such as protein modification and plant-pathogen interactions. Co-infection boosted these effects, triggering strong increases in defense-related TF, e.g., WRKYs, NACs, MYCs, and hormone signaling. Metabolite analysis showed major changes in hormones, especially cytokinins and jasmonates, with zeatin production as a key shared pathway. Weighted Gene Co-expression Network Analysis (WGCNA) identified unique gene groups for each infection, with co-infection showing enhanced immune and metabolic activity. Integrated gene-metabolite networks confirmed PVY leading role, linking key genes, e.g., AHP1/4, PYL1/8, COI1, to hormones like cytokinin, jasmonoyl-isoleucine and abscisic acid. These findings suggest PVY drives strong immune responses, while PSTVd may rely on PVY suppression of plant defenses to survive, offering new insights into managing complex plant diseases.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"232 ","pages":"Article 111117"},"PeriodicalIF":5.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146776480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic analyses in castor identify signatures of selection and loci associated with hypocotyl elongation differentiation over millennia","authors":"Jianjun Lu ,&nbsp;Lianlian Hu ,&nbsp;Wenbin Zhang ,&nbsp;Cheng Pan ,&nbsp;Donghai Li ,&nbsp;Wei Fan ,&nbsp;Jinbin Lin ,&nbsp;Songbiao Chen ,&nbsp;Peng Cui ,&nbsp;Shiyou Lü","doi":"10.1016/j.plaphy.2026.111149","DOIUrl":"10.1016/j.plaphy.2026.111149","url":null,"abstract":"<div><div>The castor, a specific source of ricinoleate oil with extensive industrial applications, has undergone millennia of global dispersal, introduction, domestication through human activities. This process has led to abundant advantageous genetic variations or phenotypic differentiations, as exemplified by the trait of hypocotyl length, which governs seedling survival and lodging resistance, thereby determining final yield. However, research on the influence of domestication on the genomic architecture of castor bean is limited, and the fundamental mechanisms underlying hypocotyl length variation in non-model crops including castor, remain largely elusive. Here, we conducted an in-depth analysis of 221 castor individuals, comprising 26 wild accessions with shorter hypocotyls from their ancestral habitats in Africa and 195 domesticated accessions with longer hypocotyls from China. Phenotypic characterization revealed that long-hypocotyl castor exhibited significantly higher elongation rates and larger epidermal cell dimensions compared to short-hypocotyl accessions. Subsequent transcriptome differential expression profiling identified a suite of candidate genes implicated in hypocotyl elongation, which were significantly enriched in hormone-mediated signaling, xyloglucan endotransglucosylase synthesis, and cellular expansion pathways. Intriguingly, selection-signature analyses revealed that, beyond the differentially expressed genes identified by transcriptome profiling, an expanded set of genomic intervals bearing selection signatures is enriched for loci regulating hypocotyl elongation, indicating their concurrent recruitment during castor domestication. Moreover, GWAS identified fifty candidate genes, including <em>CESA</em> and <em>HY5</em>, that are significantly associated with hypocotyl development. Subsequent yeast one-hybrid and mutational assays confirmed a GWAS-identified TGACT-motif variant in the <em>HY5</em> promoter as a potential functional causative site that affects hypocotyl elongation through a presumed <em>TGA-HY5</em> module. Taken together, our findings elucidate the mechanisms driving the hypocotyl length differential during domestication, provide a theoretical framework for understanding how crop traits originate and adapt to environmental change, and deliver genomic resources to accelerate castor improvement.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"232 ","pages":"Article 111149"},"PeriodicalIF":5.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146776509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous phenylalanine enhances lignin biosynthesis, reduces neck bending, and extends vase life in gerbera cut flowers","authors":"Meisam Mohammadi ,&nbsp;Ghasem Eghlima ,&nbsp;Mehdi Saidi ,&nbsp;Sunny Sharma ,&nbsp;Poonam Sharma","doi":"10.1016/j.plaphy.2026.111157","DOIUrl":"10.1016/j.plaphy.2026.111157","url":null,"abstract":"<div><div><em>Gerbera jamesonii</em> is a popular cut flower, yet its postharvest quality is often compromised by neck bending (NB) and short vase life. This study evaluated the effects of phenylalanine (Phe) at concentrations of 0, 10, 20, 40, and 80 mg L⁻¹ on the postharvest performance of ‘Ravel’ gerbera flowers during storage at 21 °C and 65–70% relative humidity over seven days. The results showed that Phe at 40 mg L⁻¹ was the most effective treatment, significantly (p &lt; 0.05) reducing NB severity by 81.7% and increasing vase life by 42.9% compared to the control (10 vs. 7 days). This concentration also enhanced vase solution uptake (4.3 vs. 2.66 mL) and maintained membrane integrity, as indicated by lower electrolyte leakage (36.3% vs. 62.8%). Phe treatment significantly reduced levels of hydrogen peroxide, malondialdehyde, and proline, while preserving higher total protein content (0.69 vs. 0.43 g kg⁻¹). It also enhanced antioxidant enzyme activities, including catalase, peroxidase, and superoxide dismutase. Furthermore, Phe-treated flowers retained higher total phenol and flavonoid levels, increased Pheammonia-lyase activity, and exhibited reduced polyphenol oxidase activity. Lignin content in stems was significantly higher in treated flowers (353.23 vs. 240.86 mg kg⁻¹), accompanied by elevated activity of key lignin biosynthesis enzymes cinnamyl alcohol dehydrogenase and cinnamoyl-CoA reductase. In conclusion, Phe at 40 mg L⁻¹ effectively enhances the postharvest quality of cut gerbera by reducing oxidative stress, strengthening antioxidant defense, and promoting lignin biosynthesis. These findings offer a practical approach to extend vase life and minimize structural failure in commercial floriculture.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"232 ","pages":"Article 111157"},"PeriodicalIF":5.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147309418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SlBBX20 is a regulator of plant development in response to shade in tomato","authors":"M.A. Mejía ,&nbsp;G. Gómez-Ocampo ,&nbsp;C.E. Barraza ,&nbsp;A.L. Medina-Fraga ,&nbsp;E.L. Ploschuk ,&nbsp;S.D. Reposi ,&nbsp;Marina Gotelli ,&nbsp;I. Petrik ,&nbsp;J. Oklestkova ,&nbsp;G. Ponciano ,&nbsp;Bruno Silvestre Lira ,&nbsp;M. Rossi ,&nbsp;J.F. Botto","doi":"10.1016/j.plaphy.2026.111177","DOIUrl":"10.1016/j.plaphy.2026.111177","url":null,"abstract":"<div><div>An early light signal detected by plants is the reduction of red/far-red ratio (R/FR), triggering the shade avoidance syndrome (SAS), a set of developmental, growth, and biochemical responses that improve plant competition for light. B-box (BBX) transcription factors are key regulators of light-mediated responses in <em>Arabidopsis thaliana</em>; however, their roles in crop species remain elusive. Here, we investigated the function of SlBBX20 (Solyc12g089240) in the SAS of tomato (<em>Solanum lycopersicum,</em> cv. Micro-Tom) by a deep physiological and photosynthesis characterization with biochemical, phytohormone and gene expression analysis in leaves and stems. We conducted simulated shade experiments by reducing the R/FR with the addition of FR light horizontally, using green filters, and increasing plant density. Wild-type (WT) plants exhibited significant shade-avoidance responses in plant height and internode length, whereas <em>Slbbx20</em> mutant plants showed reduced SAS. In WT plants, the expression of <em>SlBBX20</em> was inhibited by low R/FR, and shade-induced genes were significantly promoted in the stems but not in the leaves. The expression of <em>SlPAR1</em> and auxin-related (<em>SlIAA7, SlIAA14</em> and <em>SlIAA19)</em> genes was significantly reduced in the <em>Slbbx20</em> mutant under low R/FR. These results correlated with the lower levels of IAA auxin phytohormone in the <em>Slbbx20</em> leaves. Further, <em>Slbbx20</em> plants produced lower levels of anthocyanins than WT under high R/FR with constitutive low expression of <em>SlCHS1, SlCHS2</em> and <em>SlFLS</em> genes in leaves. In greenhouse experiments with natural radiation, <em>Slbbx20</em> plants showed lower photosynthesis and stomatal conductance than WT under sunlight, correlated with a lower number of stomata and fruit production. These findings suggest that SlBBX20 is a regulator of light responses, improving the fine-tuning of plant growth in different light environments with high and low R/FR ratios.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"232 ","pages":"Article 111177"},"PeriodicalIF":5.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147348998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SgPAP10b-mediated phospholipid degradation under acidic soil conditions: a mechanism for high phosphorus utilization efficiency in the green manure crop Stylosanthes guianensis","authors":"Ranran Xu ,&nbsp;Jinhui Lv ,&nbsp;Chun Liu ,&nbsp;Guodao Liu ,&nbsp;Yuanhang Wu ,&nbsp;Rui Huang ,&nbsp;Pandao Liu","doi":"10.1016/j.plaphy.2026.111185","DOIUrl":"10.1016/j.plaphy.2026.111185","url":null,"abstract":"<div><div><em>Stylosanthes guianensis</em> (stylo), an important tropical and subtropical green manure crop, shows remarkable adaptation to acidic soils with low phosphate (Pi) availability. Nevertheless, the specific mechanisms underlying its high phosphorus (P) utilization efficiency (PUE) in acidic soils are not fully understood. This study combined a field experiment conducted in low-Pi acidic soils with multifaceted analyses to compare the physiological and molecular responses of two stylo genotypes differing in PUE. The high-PUE genotype P457 was superior to the low-PUE genotype Reyan No.2 in acidic soils, exhibiting significantly greater biomass, P content, and PUE. Notably, P457 displayed higher leaf acid phosphatase (APase) activity than Reyan No.2. Untargeted metabolomic and lipidomic analyses further revealed significantly lower phospholipid levels in P457 leaves than in Reyan No.2. Transcriptomic analysis identified significantly elevated expression of a purple APase gene (<em>SgPAP10b</em>) in P457 leaves than in those of Reyan No.2. Functional characterization of <em>SgPAP10b</em> via heterologous overexpression in Arabidopsis demonstrated that it not only enhanced shoot biomass, PUE, and APase activity but also reduced shoot phospholipid levels relative to wild-type plants. Recombinant SgPAP10b exhibited phosphatase activity and high hydrolytic activity toward phospholipids, including phosphatidylethanolamine and lysophosphatidylcholine. Taken together, our results suggest that <em>SgPAP10b</em>-mediated lipid remodeling and P recycling are associated with the superior low-Pi adaptability of P457, thereby contributing to improved PUE. These findings uncover a previously unrecognized role of <em>SgPAP10b</em> in stylo adaptation to low-Pi conditions and highlight it as a promising target for enhancing PUE in crops grown on acidic soils.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"232 ","pages":"Article 111185"},"PeriodicalIF":5.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147349064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous melatonin decreased Cd2+ accumulation inside cells through the phenylpropanoid biosynthesis and reduced cell damage in rice (Oryza sativa) seedlings under CdCl2 stress","authors":"Qi Wang ,&nbsp;Shenglong Nie ,&nbsp;Runzhe Zhang ,&nbsp;Yitong Yin ,&nbsp;Qiulai Song ,&nbsp;Zihao Shen ,&nbsp;Xiannan Zeng ,&nbsp;Shaokun Zhang ,&nbsp;Boyuan Chen ,&nbsp;Sun Yu ,&nbsp;Tianning Zhuang ,&nbsp;Conghe Liu ,&nbsp;Di Cao ,&nbsp;Xu Gao ,&nbsp;Quanxi Liang ,&nbsp;Yanjiang Feng ,&nbsp;Qi Zhang","doi":"10.1016/j.plaphy.2026.111171","DOIUrl":"10.1016/j.plaphy.2026.111171","url":null,"abstract":"<div><div>Heavy metal-induced stress is an abiotic form of stress that significantly restricts crop yield and quality. This stress affects plants at all stages, but they are particularly vulnerable as seedlings, when it can directly influence later growth and development. Cd²⁺ is an important heavy metal stressor and negatively influences plant growth. However, the regulation mechanism underlying Cd²⁺ stress resistance has not been adequately elucidated, especially in major cultivars, which restricts the application of Cd²⁺ resistance. Here, exogenously applied melatonin (N-acetyl-5-methoxytryptamine) was tested on rice seedlings as a practical solution to enhance the plants' stress tolerance. The modern variety Longjing 203 was used for the experiments due to its extensive cultivation in Heilongjiang Province, China. Seedlings were treated with 50μMol/L CdCl₂ and 100μMol/L exogenous melatonin to investigate the molecular mechanism underlying exogenous melatonin's ability to enhance Cd²⁺ tolerance. The results revealed that Cd²⁺-induced stress limited growth, while melatonin alleviated the stress-induced damage on seedlings. Specifically, differentially expressed gene (DEGs) analysis showed that the phenylpropanoid biosynthesis pathway was enriched in plants treated with melatonin, which was also verified by qRT-PCR, enriched enzyme activity assays, and molecular docking. Also, the results of lignin content and Cd²⁺ distribution in subcellular compartments indicated that melatonin promoted lignin accumulation and intercepted Cd²⁺ into the cell wall, limiting influx into organelles and the cytoplasm. Then, the group of applied melatonin had shown to enhance stress tolerance by reducing DNA damage, as evidenced by the DNA cross-linking, 8-hydroxy-20-deoxyguanine levels, relative density of apurinic sites, and random amplified polymorphic DNA (RAPD) analysis. These findings also revealed that exogenous melatonin relieved cellular damage caused by Cd²⁺ by reinforcing the cell wall lignin barrier to regulate cellular homeostasis.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"232 ","pages":"Article 111171"},"PeriodicalIF":5.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147355915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plastid glucose-6-phosphate dehydrogenase 3 is involved in root system reshaping under low phosphorus stress through regulating auxin and cytokinin signaling in Arabidopsis","authors":"Yunchuan Zhang ,&nbsp;Lili Yan ,&nbsp;Mengjiao Ruan ,&nbsp;Hao Sun ,&nbsp;Junjie Li ,&nbsp;Wenliang He ,&nbsp;Xiaofan Na ,&nbsp;Yurong Bi ,&nbsp;Xiaomin Wang","doi":"10.1016/j.plaphy.2026.111113","DOIUrl":"10.1016/j.plaphy.2026.111113","url":null,"abstract":"<div><div>Phosphorus (P) deficiency severely limits crop yield. Plastid glucose-6-phosphate dehydrogenase 3 (G6PD3) is extensively involved in plant adaptation to abiotic stresses. However, little is known regarding the G6PD3 roles in plant adaptation to low P environments. Among G6PD family gene mutants, <em>g6pd3</em> seedlings have the shortest primary root length under low P stress. <em>G6PD3</em> transcription was markedly induced by low P stress, especially in the meristematic and elongation zones of primary roots and lateral root primordia. <em>G6PD3</em> mutation increased the lateral root number but decreased the primary root length and the root/shoot ratio compared with WT, <em>G6PD3</em> overexpression lines, disturbing root system architecture (RSA) reshaping induced by low P conditions. <em>g6pd3</em> plants also exhibited other low P-sensitive phenotypes, such as high hydrogen peroxide (H₂O₂) levels and NADP⁺/NADPH ratio, reduced biomass, and delayed seed germination. qRT-PCR results further showed that the transcriptions of P-starvation responsive (PSR) genes (<em>PHR1</em>, <em>Pht1;4/PT2</em> and <em>Pht1;1/PT1</em>) were markedly down-regulated in <em>g6pd3</em> roots. Meanwhile, <em>G6PD3</em> mutation down-regulated the expressions of genes related to auxin (IAA) synthesis, polar transport and signaling pathway, but up-regulated the expressions of cytokinin (CTK) synthetic genes under low P stress. This ultimately resulted in low IAA levels and high CTK levels in <em>g6pd3</em> roots. Exogenous application of reduced glutathione (GSH) effectively alleviated the inhibition of primary root growth in <em>g6pd3</em> seedlings under low P stress. Taken together, <em>G6PD3</em> mutation disturbes RSA reshaping through affecting plant hormone (IAA and CTK) signals and H₂O₂ homeostasis, ultimately increasing the sensitivity of <em>Arabidopsis</em> to low P environments.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"232 ","pages":"Article 111113"},"PeriodicalIF":5.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146192691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distinct transcriptional programs control polyethylene glycol (PEG)-induced drought stress responses in oat (Avena sativa L.) shoot and roots","authors":"Mateus Simionato da Silva ,&nbsp;Luciano Carlos da Maia ,&nbsp;Bruna Miranda Rodrigues ,&nbsp;Vera Quecini ,&nbsp;Antonio Costa de Oliveira ,&nbsp;Camila Pegoraro","doi":"10.1016/j.plaphy.2026.111123","DOIUrl":"10.1016/j.plaphy.2026.111123","url":null,"abstract":"<div><div>Oats (<em>Avena sativa</em> L.) are generally considered tolerant to unfavorable environmental conditions, although drought is known to impose yield losses. Several breeding programs worldwide aim at producing new oat genotypes tolerant to water deficit, but the molecular mechanisms underlying drought responses remain scarcely characterized. We investigated the growth and biomass production of 12 oat genotypes submitted to dehydration induced by PEG. Shoot elongation and biomass production were severely impaired by osmotic stress, whereas in roots growth and dry weight were mostly increased. To gain further insight into the responses, seedlings from ‘URS Altiva’ were subjected to osmotic stress for seven days, their growth and biomass performance investigated, and the transcriptome was determined for the shoots and roots of control and water-stressed plants. Distinct transcriptional programs were demonstrated to control dehydration responses in shoots and roots, agreeing with the phenotypic responses. Photosynthesis and chloroplast assembly pathways were negatively affected in the shoots, whereas in the roots the transcription of defense genes was mostly impaired. The salvage pathways induced by osmotic stress in oat shoots and roots were shared, consisting of water deprivation and abscisic acid-mediated pathways. Candidate genes and transcription factors regulating these pathways in response to dehydration were identified. Three modules of co-regulated genes were demonstrated to be correlated with biomass production in the shoots and roots and shoot elongation. This work contributes to the current understanding of the molecular mechanisms underlying the differential response of shoots and roots to dehydration and may provide tools to develop new tolerant cultivars.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"232 ","pages":"Article 111123"},"PeriodicalIF":5.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146158135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR/Cas9-mediated SiZAT12 mutagenesis enhances drought tolerance without yield penalty in foxtail millet (Setaria italica)","authors":"Xuan Zhou ,&nbsp;Lingqian Zhang ,&nbsp;Hejing Wu ,&nbsp;Haodong Wang ,&nbsp;Jiayi Chen ,&nbsp;Xueting Kang ,&nbsp;Jianhong Hao ,&nbsp;Hongzhi Wang ,&nbsp;Lulu Gao ,&nbsp;Guanghui Yang ,&nbsp;Xiangyang Yuan ,&nbsp;Jia-Gang Wang ,&nbsp;Xiao-qian Chu","doi":"10.1016/j.plaphy.2026.111096","DOIUrl":"10.1016/j.plaphy.2026.111096","url":null,"abstract":"","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"232 ","pages":"Article 111096"},"PeriodicalIF":5.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146122655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional characterization reveals MiNAC25 and MiSGR1 as key regulators of chlorophyll degradation in mango","authors":"Xiao Du ,&nbsp;Xueyu Cui ,&nbsp;Wenping Zeng ,&nbsp;Yujiao Peng ,&nbsp;Qianfu Chen ,&nbsp;Yerong Wang","doi":"10.1016/j.plaphy.2026.111095","DOIUrl":"10.1016/j.plaphy.2026.111095","url":null,"abstract":"<div><div>Chlorophyll degradation is crucial for fruit ripening and coloration, but its transcriptional regulation in mango (<em>Mangifera indica</em> L.) remains unclear. Here, we investigated two mango cultivars, ‘Guire 82’ (persistent green) and ‘Neelum’ (yellowing), and integrated metabolomic and transcriptomic analyses implicated porphyrin and chlorophyll metabolism as the central pathway underlying peel color divergence. Weighted gene co-expression network analysis (WGCNA) identified key modules linked to pigmentation, from which we uncovered that a NAC-family transcription factor <em>MiNAC25</em> as a central regulator within the chlorophyll degradation network. <em>MiSGR1</em> a key chlorophyll catabolic gene, showed co-expression with <em>MiNAC25,</em> and <em>in silico</em> analysis revealed potential NAC-binding sites in its promoter. Subcellular localization confirmed the nuclear localization of <em>MiNAC25</em> and the chloroplast localization of <em>MiSGR1</em>. Functional validation in tomato demonstrated that heterologous overexpression of either <em>MiNAC25</em> or <em>MiSGR1</em> significantly accelerated chlorophyll degradation and up-regulated the expression of endogenous chlorophyll catabolic genes (<em>SlPPH</em>, <em>SlPAO</em>, <em>SlRCCR</em>). Notably, <em>MiNAC25</em> overexpression also activated the tomato <em>SGR</em> ortholog. Our findings reveal a previously uncharacterized transcriptional module in which the nuclear <em>MiNAC25</em> potentially coordinates chlorophyll breakdown, possibly through influencing <em>MiSGR1</em> and other catabolic genes, to govern peel yellowing in mango. This study provides key insights into the regulatory mechanism of fruit coloration and identifies <em>MiNAC25</em> and <em>MiSGR1</em> as strategic targets for improving mango fruit quality.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"232 ","pages":"Article 111095"},"PeriodicalIF":5.7,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146122705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}