Plant Physiology and Biochemistry最新文献_第4页

Arbuscular mycorrhizal inoculation enhances Robinia pseudoacacia performance in zinc-contaminated soils via improved nutrient use and stress mitigation 丛枝菌根接种通过改善养分利用和缓解胁迫提高刺槐在锌污染土壤中的生长性能

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-12-31 DOI: 10.1016/j.plaphy.2025.111008

Yu Zhang , Xiao Lou , Hui Chen , Tingying Xu , Ming Tang

Arbuscular mycorrhizal fungi (AMF) are recognized for their ability to mitigate the effects of zinc (Zn) stress in plants, though the underlying mechanisms are not fully understood. In this study, Robinia pseudoacacia seedlings were exposed to varying levels of Zn stress (0, 500, and 1000 mg kg⁻¹) with or without inoculation of Rhizophagus irregularis to explore how AMF symbiosis affects Zn uptake, phosphorus (P) acquisition, abscisic acid (ABA) distribution, and photosynthetic performance. Compared with the control, 500 mg kg⁻¹ Zn reduced shoot and root biomass by 35 % and 28 %, respectively, whereas 1000 mg kg⁻¹ Zn caused stronger inhibition, with reductions of 60.8 % and 56.0 %. Root P decreased by 25 % and 40 %, ABA distribution was altered, and photosynthetic rate and PSII efficiency declined. AMF inoculation mitigated these effects: Zn accumulation in roots and shoots decreased by 35 %, with higher root-to-shoot Zn ratios, which indicates enhanced Zn sequestration in roots. AMF-colonized plants also showed improved P uptake (40.4 %–75.1 %), increased ABA levels in roots (52.5 %–169.6 %), and alleviated reductions in biomass, net photosynthetic rate, and PSII efficiency under Zn stress. Additionally, the expression of photosynthesis-related genes (RprbcS, RppsbA, and RppsbD) was significantly upregulated in AMF-colonized plants. These findings suggest that AMF enhance tolerance to Zn stress in Robinia pseudoacacia by limiting Zn translocation to shoots, improving P acquisition, modulating ABA allocation, and maintaining photosynthetic efficiency.

丛枝菌根真菌（AMF）因其减轻植物锌（Zn）胁迫的能力而被公认，尽管其潜在的机制尚不完全清楚。在本研究中，在接种或不接种不规则根噬菌的情况下，将刺槐幼苗暴露在不同水平的Zn胁迫（0、500和1000 mg kg−1）下，探讨AMF共生如何影响Zn吸收、磷(P)获取、脱落酸（ABA）分布和光合性能。与对照相比，500 mg kg−1 Zn处理的茎部和根系生物量分别减少35%和28%，而1000 mg kg−1 Zn的抑制作用更强，分别减少60.8%和56.0%。根系磷含量下降25%和40%，ABA分布发生改变，光合速率和PSII效率下降。接种AMF减轻了这些影响：根和梢的锌积累量下降了35%，根与梢的锌比更高，这表明根对锌的吸收增强了。在锌胁迫下，amf定殖植株对磷的吸收提高了40.4% ~ 75.1%，根部ABA含量提高了52.5% ~ 169.6%，生物量、净光合速率和PSII效率的降低有所缓解。此外，在amf定殖植物中，光合作用相关基因（rprbc、RppsbA和RppsbD）的表达显著上调。上述结果表明，AMF通过限制Zn向芽部转运、促进P的获取、调节ABA分配和维持光合效率等途径增强刺槐对Zn胁迫的耐受性。

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引用次数: 0

miR156j-3p negatively regulates rice salt tolerance via ROS scavenging and ABA signaling miR156j-3p通过ROS清除和ABA信号负调控水稻耐盐性

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-12-31 DOI: 10.1016/j.plaphy.2025.111009

Shengxue Shao , Guanjie Zhang , Yao Wan , Deping Zhu , Shanshan Chen , Qiang Liu , Lili Shi , Yaqian Wei , Chonghua Wei , Dina Wei , Weiwei Gao

MicroRNAs (miRNAs) are crucial regulators of abiotic stress responses in plants. Among them, miR156 is a highly conserved miRNA family with two mature isoforms: miR156-5p and miR156-3p. While miR156-5p has been extensively studied for its functions in plant growth and stress responses, the roles of miR156-3p remain less explored. In this study, we illustrated that miR156j-3p negatively regulated rice salt tolerance by targeting OsRePRP1.2. Genetic evidence showed that miR156j-3p downregulation (STTM156j-3p) plants exhibited enhanced salt tolerance. Furthermore, OsRePRP1.2 was confirmed as a direct target of miR156j-3p via 5′ RNA ligase-mediated rapid amplification of cDNA ends (5′-RLM-RACEs) assay in vivo and a rapid transient assay in tobacco. Consistently, OsRePRP1.2 positively regulated rice salt tolerance. Furthermore, both STTM156j-3p and OsRePRP1.2 overexpression (RePRP1.2-ox) plants showed enhanced hypersensitivity to exogenous ABA treatment. Besides, physiological assays revealed that the enhanced salt tolerance was associated with reduced accumulation of hydrogen peroxide (H₂O₂) and malondialdehyde (MDA), along with elevated antioxidant enzyme activities. Finally, we found that the genes involved in ABA signaling, ABA biosynthetic and ROS-scavenging system were up-regulated in STTM156j-3p and RePRP1.2-ox plants. Taken together, our findings strongly suggest that miR156j-3p targets OsRePRP1.2 and negatively regulates rice salt tolerance through the ROS scavenging and ABA signaling pathways.

MicroRNAs （miRNAs）是植物非生物胁迫反应的重要调控因子。其中，miR156是一个高度保守的miRNA家族，有两个成熟的同工型：miR156-5p和miR156-3p。虽然miR156-5p在植物生长和胁迫反应中的功能已经被广泛研究，但miR156-3p的作用仍然很少被探索。在本研究中，我们发现miR156j-3p通过靶向OsRePRP1.2负向调控水稻耐盐性。遗传证据表明，miR156j-3p下调基因（STTM156j-3p）的植株耐盐能力增强。此外，通过5 ' RNA连接酶介导的cDNA末端快速扩增（5 ' - rml - races）体内实验和烟草快速瞬态实验，证实OsRePRP1.2是miR156j-3p的直接靶点。与此一致，OsRePRP1.2正调控水稻耐盐性。此外，STTM156j-3p和OsRePRP1.2过表达（RePRP1.2-ox）的植株对外源ABA处理的超敏性增强。此外，生理分析显示，耐盐性增强与过氧化氢（H2O2）和丙二醛（MDA）积累减少以及抗氧化酶活性升高有关。最后，我们发现在STTM156j-3p和RePRP1.2-ox植物中，与ABA信号通路、ABA生物合成和ros清除系统相关的基因均上调。综上所述，我们的研究结果强烈表明，miR156j-3p靶向OsRePRP1.2，并通过ROS清除和ABA信号通路负调控水稻耐盐性。

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引用次数: 0

Identification of ARF members in Fragaria vesca and the role of FvARF2 in auxin-induced early-stage fruit development 花椒ARF成员的鉴定及FvARF2在生长素诱导的早期果实发育中的作用

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-12-31 DOI: 10.1016/j.plaphy.2025.111013

Jiahui Feng, Ruian Zhou, Xiao Zhang, Zhihong Zhang, Yuexue Liu

Auxin response factors (ARFs) are crucial transcriptional regulators involved in the auxin pathway that control auxin-induced gene transcription during plant growth and development. This study identified 18 FvARF genes distributed disproportionately across seven chromosomes and classified into five distinct phylogenetic clades. All the FvARF proteins contained the conserved B3 and Auxin_resp domains, and 11 of them possessed the Aux/IAA domain. Further bioinformatics analysis revealed their consistency and differentiation in their sequence characteristics. Cis-element analysis predicted that FvARF1a, FvARF2, FvARF9, FvARF16b, FvARF16c, and FvARF19a promoters contained auxin-responsive elements. Transcriptome data revealed that expression levels of several FvARF genes—FvARF2, FvARF4, FvARF6a, and FvARF8—were higher in the pith and cortex of early-stage strawberry fruit. qRT-PCR indicated the elevated expression of FvARF2 at stage 3 (6–7 days post-anthesis [DPA]). Additionally, the FvARF2 gene responded to exogenous auxin. The function of FvARF2 in early strawberry fruit development was confirmed through transient transformation assays. Collectively, these results provide a comprehensive characterisation of ARF gene family members in strawberry, with functional evidence implicating FvARF2 in regulating early-stage fruit development.

生长素反应因子（ARFs）是植物生长发育过程中调控生长素诱导基因转录的重要转录调控因子。本研究确定了18个FvARF基因不成比例地分布在7条染色体上，并将其划分为5个不同的系统发育支系。所有FvARF蛋白均含有保守的B3和Auxin_resp结构域，其中11个具有Aux/IAA结构域。进一步的生物信息学分析揭示了它们序列特征的一致性和差异性。顺式元件分析预测FvARF1a、FvARF2、FvARF9、FvARF16b、FvARF16c和FvARF19a启动子含有生长素响应元件。转录组数据显示，FvARF基因fvarf2、FvARF4、FvARF6a和fvarf8在草莓早期果实的髓和皮层中表达水平较高。qRT-PCR显示FvARF2在第3期（花后6-7天[DPA]）表达升高。此外，FvARF2基因对外源性生长素有反应。通过瞬时转化试验，证实了FvARF2在草莓果实早期发育中的作用。总的来说，这些结果提供了草莓中ARF基因家族成员的全面特征，并提供了FvARF2在调节早期果实发育中的功能证据。

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引用次数: 0

DIA-based quantitative proteomics reveals the mechanism of foliar spraying glycine betaine and zinc in improving the quality of dry-cultivated rice 基于遗传分析法的定量蛋白质组学研究揭示了叶面喷施甜菜碱和锌改善旱作水稻品质的机理。

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-12-30 DOI: 10.1016/j.plaphy.2025.110982

Guan Wang , Qianqian Liu , Zixian Jiang , Xinru Shen , Hao Jiang , Xudong Yang , Guangxin Zhao , Xiaoshuang Wei , Meiying Yang , Zhihai Wu

This study used DIA-based proteomics to elucidate how foliar application of glycine betaine (GB) and zinc (Zn) synergistically regulates quality formation in dry-cultivated rice. GB and Zn effectively improved both the appearance and eating quality of rice through multi-level coordinated actions. GB primarily enhanced drought tolerance and quality by regulating key protein expression: upregulated RRM domain proteins potentially directly regulated starch and protein synthesis; increased disease resistance proteins activated immune priming, establishing a dual “drought tolerance-immunity” protective system; SNF2 chromatin remodeling proteins participated in epigenetic reprogramming; and amino acid transporters optimized nitrogen allocation. Zn induced functional proteins that enhancedmetabolic homeostasis: Pyridoxal 5-phosphate synthase promoted antioxidant synthesis; dCTP pyrophosphatase and tRNA pseudouridine synthase ensured genomic stability and accurate protein synthesis; and 3-isopropylmalate dehydratase promoted the accumulation of flavor precursors. The combined application of GB and Zn produced significant synergistic effects: upregulated Hexosyltransferase served as a key node, likelystabilizing osmolytes and antioxidants via glycosylation modifications and participating in zinc ligand synthesis to facilitate zinc transport and grain enrichment. Persistent upregulation of Receptor-like serine/threonine-protein kinase (RLK) enhanced signal perception and transduction, thereby initiating drought-resistance defense pathways. Physiologically, combined foliar application significantly enhanced photosynthetic, promoted osmolyte accumulation, and increased antioxidant enzyme activities. Overall, GB stabilizes functional proteins, while Zn servesas a cofactor to activate enzymatic activity. Together, they create a"signal perception-defense activation-nutrition optimization” pathway that mitigates oxidative damage while enhancing stress resistance and quality in dry-cultivated rice, providing a theoretical foundationfor cultivating stress-resistant and high-quality crops.

本研究利用基于dia的蛋白质组学研究了叶片施用甘氨酸甜菜碱（GB）和锌（Zn）对旱作水稻品质形成的协同调节作用。GB和Zn通过多层次的协同作用，有效地改善了稻米的外观和食用品质。GB主要通过调节关键蛋白的表达来提高耐旱性和品质：上调RRM结构域蛋白可能直接调控淀粉和蛋白质的合成；增加抗病蛋白激活免疫启动，建立“耐旱-免疫”双重保护体系；SNF2染色质重塑蛋白参与表观遗传重编程；氨基酸转运体优化氮分配。锌诱导功能蛋白增强代谢稳态：吡哆醛5-磷酸合成酶促进抗氧化剂合成；dCTP焦磷酸酶和tRNA假尿嘧啶合成酶确保了基因组的稳定性和准确的蛋白质合成；3-异丙基苹果酸脱水酶促进风味前体的积累。GB和Zn的联合施用产生了显著的协同效应：上调己糖基转移酶作为关键节点，可能通过糖基化修饰稳定渗透物和抗氧化剂，参与锌配体的合成，促进锌的运输和谷物富集。受体样丝氨酸/苏氨酸蛋白激酶（RLK）的持续上调增强了信号感知和转导，从而启动了抗旱防御途径。生理上，叶面配施显著提高了光合作用，促进了渗透物积累，提高了抗氧化酶活性。总的来说，GB稳定了功能蛋白，而Zn则作为激活酶活性的辅助因子。他们共同创造了一个“信号感知-防御激活-营养优化”的途径，减轻了旱作水稻的氧化损伤，同时提高了抗逆性和品质，为培育抗逆性和优质作物提供了理论基础。

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引用次数: 0

Comparative study of the Dof gene family in buckwheat: FtDof34's function in sugar biosynthesis and response to salt and drought stress 荞麦Dof基因家族的比较研究：FtDof34在糖生物合成中的作用及对盐和干旱胁迫的响应

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-12-30 DOI: 10.1016/j.plaphy.2025.111003

Rintu Jha , Wei Li , Hao Lin , Yuqi He , Jia Gao , Kaixuan Zhang , Meihong Shao , Meiliang Zhou

Dof (DNA binding with one finger) type transcription factors play a key role in response to abiotic stress in plants. However, its functional role in buckwheat remains unexplored. Here, we conducted a comprehensive analysis of the Dof-gene family across three buckwheat species, revealing segmental duplication as the primary driver of family expansion and strong purifying selection during evolution. A FtDof34 gene, was identified through genome-wide association analysis and found to be associated with soluble sugar 1,1-kestotetraose content. Functional validation in A. thaliana and hairy root of Tartary buckwheat demonstrated that overexpression of this gene may promotes soluble sugar biosynthesis and enhances plant resistance to salt and drought stress. This study clarified the regulatory mechanisms of the Dof family in buckwheat species, elucidating the regulatory mechanisms of FtDof34 gene in regulating the biosynthesis of sugar metabolites and stress (salt and drought) resistance, offering potential genetic resources for breeding buckwheat varieties with superior agronomic traits.

Dof （DNA binding with one finger）型转录因子在植物对非生物胁迫的响应中起着关键作用。然而，其在荞麦中的功能作用仍未被探索。在此，我们对三个荞麦物种的dof基因家族进行了全面分析，揭示了片段复制是家族扩展和进化过程中强烈的净化选择的主要驱动因素。通过全基因组关联分析，鉴定出FtDof34基因与可溶性糖1,1-酮四糖含量相关。在拟南芥和苦荞毛状根中的功能验证表明，该基因的过表达可促进可溶性糖的生物合成，增强植物对盐和干旱胁迫的抗性。本研究阐明了Dof家族在荞麦中的调控机制，阐明了FtDof34基因调控糖代谢物生物合成和逆境（盐和干旱）抗性的调控机制，为选育具有优良农艺性状的荞麦品种提供了潜在的遗传资源。

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引用次数: 0

The MdARF7/19-MdBBM1 module mediates auxin signaling to regulate the formation of adventitious roots in self-rooted apple stocks MdARF7/19-MdBBM1模块介导生长素信号，调控苹果自根砧木不定根的形成

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-12-30 DOI: 10.1016/j.plaphy.2025.111004

Zenghui Wang , Wei Liu , Haixia Tang , Lijuan Feng , Yanlei Yin , Jialin Li

Self-rooted apple stocks are the main recommended stocks for high-standard orchard construction in China. However, the few adventitious roots (ARs) in self-rooted apple stocks have restricted the high-quality development of the apple industry. BABY BOOM (BBM) plays a key role in the regeneration and transformation of plants. BBM1 was known to regulate ARs formation, was affected by auxin. However, the role of MdBBM1 in the formation of self-rooted apple stocks ARs remains unclear. Therefore, it is particularly necessary to clarify the molecular mechanism of MdBBM1-mediated ARs formation in self-rooted apple stocks. In this study, the MdARF7 and MdARF19 were revealed, two auxin-responsive transcription factors in response to auxin signaling, directly activate MdBBM1 expression via bind to its promoter, thereby promoting the self-rooted apple stocks ARs formation. Overexpression of MdBBM1 significantly enhances ARs development in self-rooted apple stocks, confirming its functional importance. Furthermore, we demonstrate that MdARF7 interacts with MdRH53, suggesting an additional regulatory layer in adventitious root formation. Collectively, our findings establish a novel auxin-MdARF7/19-MdBBM1 regulatory module that regulates ARs development in self-rooted apple stocks.

自根苹果是中国高标准果园建设的主要推荐树种。然而，自根苹果存量中不定根较少，制约了苹果产业的高质量发展。BABY BOOM （BBM）在植物的再生和转化过程中起着关键作用。BBM1调节ARs的形成，受生长素影响。然而，MdBBM1在苹果自根股ARs形成中的作用尚不清楚。因此，阐明mdbbm1介导的苹果自根砧木ARs形成的分子机制就显得尤为必要。本研究揭示了两个生长素应答转录因子MdARF7和MdARF19响应生长素信号，通过与其启动子结合直接激活MdBBM1的表达，从而促进苹果砧木自根ARs的形成。过表达MdBBM1显著促进了自根苹果砧木中ARs的发育，证实了其功能的重要性。此外，我们证明MdARF7与MdRH53相互作用，表明在不定根形成中存在额外的调控层。总之，我们的研究结果建立了一个新的生长素- mdarf7 /19- mdbbm1调控模块，该模块调节自根苹果砧木中ARs的发育。

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引用次数: 0

Rhizosphere regulation by three Bacillus species and tomato productivity: A feasible approach for moderately saline-alkali soil remediation 三种芽孢杆菌根际调控与番茄产量：中盐碱土壤修复的可行途径

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-12-30 DOI: 10.1016/j.plaphy.2025.111005

Yu-Miao Yang, Yu-Kun Chen, Xiao-Fang Gong, Jian-Jun Yang, Jin-Hao Du, Ying Zhu

Soil salinization can restrict the cultivation of plants. Utilizing plant growth-promoting bacteria (PGPB) to aid plant growth and saline-alkali land improvement, which is one of the hotspots in agricultural green development and global food security. This study employed three Bacillus species of B. amyloliquefaciens, B. licheniformis and B. subtilis as core functional microorganisms existing in a bio-organic fertilizer (BOF), to investigate the rhizospheric effects of PGPB belonging to the same genus but different species. Results showed that all the three PGPB species had successfully colonized the rhizosphere soil, enhancing the diversity of rhizosphere bacterial communities during the tomato's seedling stage. The enhancement of soil microbial activity further increased the activities of protease, sucrase, alkaline phosphatase and polyphenol oxidase, thus increasing the absorption and consumption of nitrogen and phosphorus by plants. In addition, the microbial activity and metabolites increased soil porosity and refined soil particles, thereby improving the soil physical structure. Significantly, the BOF application notably reduced soil total calcium, exchangeable calcium and water-soluble salts through the complexation of salt-based cations. Our results confirmed that the saline-alkali resistance of Bacillus species could improve soil quality and plant productivity, thereby providing novel insights into the effective application of PGPB in saline-alkali soils.

土壤盐碱化会限制植物的种植。利用植物生长促进菌（plant growth-promoting bacteria， PGPB）辅助植物生长和盐碱地改良，是农业绿色发展和全球粮食安全的热点之一。以解淀粉芽孢杆菌、地衣芽孢杆菌和枯草芽孢杆菌3种芽孢杆菌作为生物有机肥（BOF）中的核心功能微生物，研究了同一属不同种的ppgpb对根际的影响。结果表明，3种细菌均能成功定植根际土壤，提高了番茄苗期根际细菌群落的多样性。土壤微生物活性的增强进一步提高了蛋白酶、蔗糖酶、碱性磷酸酶和多酚氧化酶的活性，从而增加了植物对氮和磷的吸收和消耗。此外，微生物活性和代谢产物增加了土壤孔隙度，细化了土壤颗粒，从而改善了土壤的物理结构。BOF通过盐基阳离子的络合作用，显著降低了土壤总钙、交换性钙和水溶性盐。本研究结果证实了芽孢杆菌的耐盐碱性可以改善土壤质量和植物生产力，从而为PGPB在盐碱地的有效应用提供了新的见解。

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引用次数: 0

Arsenic metabolism in litchi chinensis and its effects on fruit quality 荔枝砷代谢及其对果实品质的影响

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-12-30 DOI: 10.1016/j.plaphy.2025.111002

Shimiao Chen , Yanyan Li , Fuhai Zheng , Qinyu Lu

This study investigates the impact of different arsenic (As) species (arsenite (As³⁺), arsenate (As⁵⁺), and their mixture) on Litchi chinensis Sonn. fruit quality and underlying mechanisms through foliar spray treatments on 3-year-old litchi trees. The results revealed that As accumulation and distribution vary significantly across tissues, with leaves and peels exhibiting 40 times higher As levels than those in the flesh. As³⁺ treatment resulted in the highest total As accumulation in fruits by the end of ripening, whereas mixed treatments prolonged the persistence of bioavailable As (particularly As³⁺), leading to sustained stress. As exposure reduced yields by an average of 36 % (As³⁺) and moderately inhibited fruit development (As⁵⁺ and mixed treatment). Mechanistically, As speciation reshaped the sugar–acid metabolic network primarily by modulating the PPC–MDH axis. The analysis revealed that As exposure upregulated PPC and MDH pathways, redirecting carbon flux toward ascorbate-derived organic acid accumulation (e.g., tartaric acid), while increased TCA cycle activity negatively regulated sugar accumulation. Mixed treatments induced complex shifts in gene expression, reflecting the combined effects of oxidative stress and metabolic perturbations. Notably, As³⁺ exhibited 10-fold higher toxicity than As⁵⁺ due to stronger thiol group binding and enzymatic inhibition. These findings underscore the risks of As contamination in litchi production, underscoring the need for monitoring As species in fertilizers and mitigating their accumulation to ensure food safety and sustainability.

研究了不同砷种类（亚砷酸盐（As3+）、砷酸盐（As5+）及其混合物）对荔枝生长的影响。3年生荔枝叶面喷雾处理的果实品质及其机制。结果表明，不同组织间砷的积累和分布差异显著，叶片和果皮的砷含量是果肉的40倍。As3+处理导致果实成熟末期总砷积累量最高，而混合处理延长了生物可利用砷（尤其是As3+）的持续时间，导致持续胁迫。砷暴露平均使产量降低36% (As3+)，中度抑制果实发育（As5+和混合处理）。在机制上，As物种形成主要通过调节PPC-MDH轴来重塑糖酸代谢网络。分析显示，暴露于砷会上调PPC和MDH途径，将碳通量转向抗坏血酸衍生的有机酸积累（如酒石酸），而TCA循环活性的增加则对糖积累产生负调控。混合处理诱导了基因表达的复杂变化，反映了氧化应激和代谢扰动的综合作用。值得注意的是，As3+的毒性比As5+高10倍，这是由于As3+更强的巯基结合和酶抑制作用。这些发现强调了荔枝生产中砷污染的风险，强调了监测肥料中砷种类并减少其积累以确保食品安全和可持续性的必要性。

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引用次数: 0

OsCBL10 negatively regulates salt tolerance at seedling stage in rice OsCBL10负调控水稻苗期耐盐性

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-12-30 DOI: 10.1016/j.plaphy.2025.110999

Xuemei Kuang , Ruofeng Liu , Lei Jin , Shengping Wang , Shufeng Gao , Yilan Qiu

Soil salinity increasingly endangers global rice production. Although CBL–CIPK complexes are known to decode salt-induced Ca²⁺surges to activate ion transporters and antioxidant pathways, the in vivo role of rice OsCBL10 remains unknown, leaving a critical gap in Ca²⁺-mediated salt-tolerance mechanisms. In this study, we demonstrate that OsCBL10 is a tonoplast-localized Ca²⁺sensor that is transcriptionally up-regulated by salinity and most highly expressed in anthers and salt-stressed shoots. CRISPR-Cas9 knockout lines (cbl10-1/2) exhibited higher survival, 25 % longer shoots and a 30 % lower shoot Na⁺/K⁺ ratio than wild-type (WT) plants under 100 mM NaCl, whereas over-expression lines (OE1/2) were hypersensitive. The improved tolerance of cbl10 mutants was linked to (i) enhanced Na⁺extrusion and K⁺retrieval, (ii) reduced H₂O₂ accumulation, and (iii) up-regulation of OsSOS1, OsNHX1 and OsMIOX. Thus, OsCBL10 functions as a tonoplast “brake” that dampens SOS signaling and ROS scavenging under salt stress. This study broadens the known roles of rice CBL proteins and highlights OsCBL10 as a key gene for engineering salt-resilient rice varieties.

土壤盐碱化日益危及全球水稻生产。虽然已知CBL-CIPK复合物可以解码盐诱导的Ca2+激增，激活离子转运体和抗氧化途径，但水稻OsCBL10在体内的作用仍然未知，这在Ca2+介导的耐盐机制中留下了一个关键的空白。在这项研究中，我们证明OsCBL10是一种定位于张力质体的Ca2+传感器，受盐度的转录上调，在花药和盐胁迫芽中表达最多。在100 mM NaCl胁迫下，CRISPR-Cas9敲除系（cbl10-1/2）的存活率比野生型（WT）高，新梢长25%，新梢Na+/K+比低30%，而过表达系（OE1/2）则极度敏感。cbl10突变体耐受性的提高与(i) Na+挤压和K+回收增强，（ii） H2O2积累减少，以及（iii） OsSOS1、OsNHX1和OsMIOX基因的上调有关。因此，OsCBL10在盐胁迫下起着抑制SOS信号和清除ROS的作用。该研究拓宽了水稻CBL蛋白的已知作用，并强调了OsCBL10作为工程盐抗性水稻品种的关键基因。

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引用次数: 0

Short-duration high blue light at night enhances phytochemicals and morphology without yield loss in three lettuce cultivars 夜间短时间强蓝光增强了三种生菜品种的植物化学物质和形态，但不造成产量损失。

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2025-12-29 DOI: 10.1016/j.plaphy.2025.111001

Laura Cammarisano , Viviana Correa Galvis , Filippos Charalambous , Elmien Heyneke , Emilio Villar Alegria , Ricky Sugiarto Teng , Oliver Körner , Pavlos Kalaitzoglou

Controlled environment agriculture (CEA) enables precise regulation of plant physiological processes through environmental control. Among these, the spectral composition of photosynthetically active radiation (PAR), particularly the proportion, dose, and duration of blue light exposure, has a strong effect in enhancing plant nutritional quality. This study investigated how end-of-production (EoP) light spectrum (PAR vs. PAR with high blue portion) and application mode (daily continuous for 18 h vs. 18 h plus 2-h nighttime exposure) affect the growth, morphology and nutritional quality of three red leaf lettuce cultivars (Lactuca sativa var. crispa ‘Xandra’, ‘Alaine’ and ‘Haflex’). Plants grown in climate-controlled conditions for 29 days, were exposed to four light treatments (Ctrl, HB, Night, NightB) with a Daily Light Integral (DLI) of 24.3 mol m⁻² d⁻¹ during the final seven days. Light spectrum and application mode significantly affected the three lettuce cultivars. Plants treated with continuous high-blue light (HB) had the lowest values for shoot height, leaf area and biomass, but greatest values for pigment content, antioxidant activity and leaf nitrate accumulation. Short-duration, nighttime blue light application (NightB) improved anthocyanins, phenolics and antioxidant activity without yield loss, achieving the highest blue light use efficiency for anthocyanins (BLUE_CYA) and biomass (BLUE_FW). Cultivar-specific responses were evident, with ‘Haflex’ exhibiting greater yield, rapid anthocyanin response and lower nitrate accumulation, highlighting the potential for genotype-tailored light strategies in CEA. These results demonstrate that concentrated blue light, when applied as short duration supplementation during the dark phase, can be a viable strategy for enhancing visual and nutritional quality of salad leaves with contained energy expense in commercial plant factories.

环境控制农业（Controlled environment agriculture， CEA）通过对环境的控制，对植物的生理过程进行精确调控。其中，光合有效辐射（PAR）的光谱组成，特别是蓝光照射的比例、剂量和持续时间，对提高植物营养品质有很强的作用。本研究研究了生产末端（EoP）光谱（PAR vs高蓝色部分PAR）和施用方式（每日连续照射18 h vs夜间照射18 h + 2 h）对三种红叶莴苣品种（lacuca sativa var. crispa ‘Xandra’、‘Alaine’和‘Haflex’）生长、形态和营养品质的影响。在气候控制条件下生长29天的植物，在最后7天暴露于四种光处理（Ctrl， HB, Night, NightB），每日光积分（DLI）为24.3 mol m-2 d-1。光谱和施用方式对3个生菜品种的生长有显著影响。连续高蓝光（HB）处理植株的茎高、叶面积和生物量最低，但色素含量、抗氧化活性和叶片硝酸盐积累量最高。夜间短时间蓝光处理（NightB）在不损失产量的情况下提高了花青素、酚类物质和抗氧化活性，实现了花青素（BLUECYA）和生物质（BLUEFW）的最高蓝光利用效率。品种特异性反应明显，“Haflex”表现出更高的产量、更快的花青素反应和更低的硝酸盐积累，突出了CEA基因型定制光策略的潜力。这些结果表明，在商业植物工厂中，当在黑暗阶段使用浓缩蓝光作为短时间补充时，可以在控制能量消耗的情况下提高沙拉叶的视觉和营养质量。

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