Plant Physiology and Biochemistry最新文献_第2页

Enhanced bacoside synthesis in Bacopa monnieri plants using seed exudates from Tamarindus indica 利用罗望子的种子渗出物增强百部皂苷的合成。

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2024-11-10 DOI: 10.1016/j.plaphy.2024.109287

Titir Guha , Ekta Bhattacharya , Madhurima Dutta , Anisha Dutta , Moumita Dandapat , Rahul Bose , Suparna Mandal Biswas

Diverse allelochemicals are released from different plant parts via leaching, exudation, volatilization, etc., which can induce either stimulatory or inhibitory effects depending on the target plant species. Very few reports provide details about allelopathic interaction through seed exudates. Since Tamarindus indica L. seed exudate (TSE) has been known to exhibit growth stimulatory effect on lettuce, radish, and sesame, in the present study we have evaluated its role in regulating the secondary metabolism of an over-exploited medicinal herb, Bacopa monnieri (L.) Pennel. The bacoside biosynthesis rate of B. monnieri is quite low in comparison to its increasingly high demands in the pharmaceutical industry. Currently, researches are aimed towards enhancing the biosynthesis of this secondary metabolite in planta by utilizing external stress factors. Presently, 7-day-old B. monnieri seedlings were treated with 1:16, 1:8, 1:4, 1:3, and 1:2 (seed weight: water) TSE. Maximum upregulation of secondary metabolite contents was found in the 1:4 (seed weight: water) TSE treatment set. This TSE treatment also enhanced H₂O₂ and salicylic acid production leading to the upregulation of the genes related to the MVA pathway (BmAACT, BmHMGR, BmMDD, BmSQS, and BmBAS) which are responsible for bacoside biosynthesis and 1.7-fold higher bacoside level was found in TSE treated set compared to control. LC-HRMS analysis of TSE confirmed the presence of alkaloid (lupanine), phenol (chlorogenic acid), and organic acid (mucic acid), which are identified as potential allelochemicals responsible for modulating the secondary metabolism of B. monnieri. Thus, this study highlights a sustainable approach towards enhancing bacoside production in planta.

植物的不同部位会通过浸出、渗出、挥发等方式释放出多种等位化学物质，根据目标植物种类的不同，这些化学物质可产生刺激或抑制作用。有关通过种子渗出物进行等位异化作用的详细报道很少。由于已知罗望子种子渗出物（TSE）对莴苣、萝卜和芝麻具有生长刺激作用，在本研究中，我们评估了它在调节一种被过度开发的药用植物百部（Bacopa monnieri (L.) Pennel）次生代谢中的作用。与制药业日益增长的需求相比，百服宁的生物合成率相当低。目前，研究的目标是通过利用外部胁迫因素来提高这种次生代谢物在植物体内的生物合成。目前，用 1:16、1:8、1:4、1:3 和 1:2（种子重量：水）的 TSE 处理 7 天大的猴面包树幼苗。在 1:4 （种子重量：水）TSE 处理组中，次生代谢物含量的上调幅度最大。这种 TSE 处理还提高了 H2O2 和水杨酸的产量，导致负责巴豆苷生物合成的 MVA 途径相关基因（BmAACT、BmHMGR、BmMDD、BmSQS 和 BmBAS）上调，TSE 处理组的巴豆苷含量比对照组高 1.7 倍。TSE的LC-HRMS分析证实了生物碱（羽扇豆碱）、酚（绿原酸）和有机酸（粘液酸）的存在，这些物质被确定为潜在的等位化学物质，负责调节猴面包树的次生代谢。因此，本研究强调了提高植物体内巴豆苷产量的可持续方法。

{"title":"Enhanced bacoside synthesis in Bacopa monnieri plants using seed exudates from Tamarindus indica","authors":"Titir Guha , Ekta Bhattacharya , Madhurima Dutta , Anisha Dutta , Moumita Dandapat , Rahul Bose , Suparna Mandal Biswas","doi":"10.1016/j.plaphy.2024.109287","DOIUrl":"10.1016/j.plaphy.2024.109287","url":null,"abstract":"<div><div>Diverse allelochemicals are released from different plant parts via leaching, exudation, volatilization, etc., which can induce either stimulatory or inhibitory effects depending on the target plant species. Very few reports provide details about allelopathic interaction through seed exudates. Since <em>Tamarindus indica</em> L. seed exudate (TSE) has been known to exhibit growth stimulatory effect on lettuce, radish, and sesame, in the present study we have evaluated its role in regulating the secondary metabolism of an over-exploited medicinal herb, <em>Bacopa monnieri</em> (L.) Pennel. The bacoside biosynthesis rate of <em>B. monnieri</em> is quite low in comparison to its increasingly high demands in the pharmaceutical industry. Currently, researches are aimed towards enhancing the biosynthesis of this secondary metabolite <em>in planta</em> by utilizing external stress factors. Presently, 7-day-old <em>B. monnieri</em> seedlings were treated with 1:16, 1:8, 1:4, 1:3, and 1:2 (seed weight: water) TSE. Maximum upregulation of secondary metabolite contents was found in the 1:4 (seed weight: water) TSE treatment set. This TSE treatment also enhanced H<sub>2</sub>O<sub>2</sub> and salicylic acid production leading to the upregulation of the genes related to the MVA pathway (<em>BmAACT</em>, <em>BmHMGR</em>, <em>BmMDD</em>, <em>BmSQS, and BmBAS</em>) which are responsible for bacoside biosynthesis and 1.7-fold higher bacoside level was found in TSE treated set compared to control. LC-HRMS analysis of TSE confirmed the presence of alkaloid (lupanine), phenol (chlorogenic acid), and organic acid (mucic acid), which are identified as potential allelochemicals responsible for modulating the secondary metabolism of <em>B. monnieri</em>. Thus, this study highlights a sustainable approach towards enhancing bacoside production <em>in planta</em>.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109287"},"PeriodicalIF":6.1,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An integrated quality, physiological and transcriptomic analysis reveals mechanisms of kiwifruit response to postharvest transport vibrational stress 综合质量、生理和转录组分析揭示了猕猴桃对采后运输振动压力的响应机制。

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2024-11-09 DOI: 10.1016/j.plaphy.2024.109285

Chenxu Zhao , Linlin Cheng , Yurong Guo , Wei Hui , Junpeng Niu , Shujie Song

The ‘Xuxiang’ kiwifruit, a leading cultivar in China known for its high quality and yield, experiences quality degradation due to vibration stress during postharvest transportation. This study simulated the postharvest transportation vibrations of ‘Xuxiang’ kiwifruits to investigate the effects on the fruit quality and physiology. Different vibration intensities (0.26, 0.79, and 1.5 m s⁻²) and durations (0, 24, 48, 72, and 96 h) were applied to analyze the quality, physiological and transcriptomic changes of fruits after vibration stress, as well as the association between quality deterioration, gene networks, and key genes. Results indicated that vibration stress significantly accelerated the deterioration of fruit quality and induced physiological changes. As vibration intensity and duration increased, there was a rapid decrease in fruit firmness and an increase in weight loss, soluble solid content, relative conductivity, ethylene production, respiratory rate, and malondialdehyde levels. The most severe deterioration in fruit quality occurred at a vibration intensity of 1.5 m s⁻². Transcriptome sequencing analysis was conducted on samples from different durations of exposure to the 1.5 m s⁻² vibration intensity. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses identified key genes associated with ethylene metabolism and softening. Weighted Gene Co-Expression Network Analysis (WGCNA) and correlation analysis further determined that 24 of these genes were regulated by vibrational stress, impacting ethylene metabolism and cell wall degradation. Vibration stress induced changes in genes related to ethylene metabolism and cell wall degradation, promoting lipid peroxidation and respiratory changes, which compromise cell membrane integrity and lead to quality deterioration. Compared with untreated fruits, vibration stress caused the quality deterioration, physiological changes and transcriptional regulation of kiwifruits, indicating that kiwifruits respond to vibration stress through multiple aspects. It proposes a fresh outlook on the understanding of the mechanism of transport vibration stress and further illustrates the importance of monitoring vibration intensity and duration as well as reducing vibration.

'徐香'猕猴桃是中国以优质高产著称的主要栽培品种，但在采后运输过程中会因振动应力而导致品质下降。本研究模拟了 "徐香 "猕猴桃采后运输振动，以研究其对果实质量和生理的影响。应用不同的振动强度（0.26、0.79 和 1.5 m s-2）和持续时间（0、24、48、72 和 96 h），分析振动胁迫后果实的品质、生理和转录组变化，以及品质恶化、基因网络和关键基因之间的关联。结果表明，振动胁迫明显加速了果实品质的恶化，并诱发了生理变化。随着振动强度和持续时间的增加，果实硬度迅速下降，重量损失、可溶性固形物含量、相对电导率、乙烯产量、呼吸速率和丙二醛含量增加。在振动强度为 1.5 m s-2 时，果实质量的恶化最为严重。对暴露于 1.5 m s-2 振动强度的不同持续时间的样品进行了转录组测序分析。京都基因组百科全书（KEGG）和基因本体（GO）富集分析确定了与乙烯代谢和软化相关的关键基因。加权基因共表达网络分析（WGCNA）和相关性分析进一步确定，这些基因中有 24 个受振动胁迫调控，影响乙烯代谢和细胞壁降解。振动胁迫诱导乙烯代谢和细胞壁降解相关基因的变化，促进脂质过氧化和呼吸变化，从而破坏细胞膜的完整性，导致品质下降。与未处理果实相比，振动胁迫导致猕猴桃品质下降、生理变化和转录调控，表明猕猴桃对振动胁迫的响应是多方面的。该研究为了解运输振动胁迫的机理提出了新的视角，并进一步说明了监测振动强度和持续时间以及减少振动的重要性。

{"title":"An integrated quality, physiological and transcriptomic analysis reveals mechanisms of kiwifruit response to postharvest transport vibrational stress","authors":"Chenxu Zhao , Linlin Cheng , Yurong Guo , Wei Hui , Junpeng Niu , Shujie Song","doi":"10.1016/j.plaphy.2024.109285","DOIUrl":"10.1016/j.plaphy.2024.109285","url":null,"abstract":"<div><div>The ‘Xuxiang’ kiwifruit, a leading cultivar in China known for its high quality and yield, experiences quality degradation due to vibration stress during postharvest transportation. This study simulated the postharvest transportation vibrations of ‘Xuxiang’ kiwifruits to investigate the effects on the fruit quality and physiology. Different vibration intensities (0.26, 0.79, and 1.5 m s<sup>−2</sup>) and durations (0, 24, 48, 72, and 96 h) were applied to analyze the quality, physiological and transcriptomic changes of fruits after vibration stress, as well as the association between quality deterioration, gene networks, and key genes. Results indicated that vibration stress significantly accelerated the deterioration of fruit quality and induced physiological changes. As vibration intensity and duration increased, there was a rapid decrease in fruit firmness and an increase in weight loss, soluble solid content, relative conductivity, ethylene production, respiratory rate, and malondialdehyde levels. The most severe deterioration in fruit quality occurred at a vibration intensity of 1.5 m s<sup>−2</sup>. Transcriptome sequencing analysis was conducted on samples from different durations of exposure to the 1.5 m s<sup>−2</sup> vibration intensity. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses identified key genes associated with ethylene metabolism and softening. Weighted Gene Co-Expression Network Analysis (WGCNA) and correlation analysis further determined that 24 of these genes were regulated by vibrational stress, impacting ethylene metabolism and cell wall degradation. Vibration stress induced changes in genes related to ethylene metabolism and cell wall degradation, promoting lipid peroxidation and respiratory changes, which compromise cell membrane integrity and lead to quality deterioration. Compared with untreated fruits, vibration stress caused the quality deterioration, physiological changes and transcriptional regulation of kiwifruits, indicating that kiwifruits respond to vibration stress through multiple aspects. It proposes a fresh outlook on the understanding of the mechanism of transport vibration stress and further illustrates the importance of monitoring vibration intensity and duration as well as reducing vibration.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109285"},"PeriodicalIF":6.1,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MYB-1 regulates anthocyanin biosynthesis in Magnolia wufengensis MYB-1 调节五峰木兰的花青素生物合成。

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2024-11-09 DOI: 10.1016/j.plaphy.2024.109283

Xian-ping Liu , Min Luo , Xiu-qi Liu , Liu-yan Hao , Chen Zhu , Li Wang , Lv-yi Ma

Anthocyanin is an essential pigment in all major horticultural crops especially in ornamental trees. Magnolia wufengensis (new species of Magnolia) with red color flower was recently found as a popular species for ornamental use, but anthocyanin synthesis and regulation in M. wufengensis are poorly understood. Herein, transcriptome analysis was used to decipher the gene network associated with anthocyanin biosynthesis. An R2R3-like MwMYB-1 transcription factor was found. MwMYB-1 overexpression resulted in anthocyanin accumulation in tobacco and Arabidopsis. MwMYB-1 worked independently rather than forming a protein complex with bHLH or WD40 protein. According to MwMYB-1 DAP-seq analysis in Arabidopsis, the MwMYB-1 transcription factor preferred to bind the “AAGAGAG” motif (DREME-5) in the third exon of the AtMYB75 gene. The yeast one hybrid assay and transcription activity assay further confirmed this. Thus, MwMYB-1 activated AtMYB75 gene expression and conducted cascade amplification of anthocyanin biosynthesis. Taken together, our findings provide a novel understanding of anthocyanin biosynthesis regulation in M. wufengensis and can be used to promote agronomic trait improvement in tree species.

花青素是所有主要园艺作物尤其是观赏树木中不可或缺的色素。花色为红色的五凤木兰（木兰属新种）是最近发现的一种常用观赏树种，但人们对五凤木兰中花青素的合成和调控知之甚少。本文利用转录组分析破译了与花青素生物合成相关的基因网络。研究发现了一种类似 R2R3 的 MwMYB-1 转录因子。MwMYB-1的过表达导致烟草和拟南芥中花青素的积累。MwMYB-1 独立工作，而不是与 bHLH 或 WD40 蛋白形成蛋白复合物。根据拟南芥中的 MwMYB-1 DAP-seq 分析，MwMYB-1 转录因子倾向于结合 AtMYB75 基因第三外显子中的 "AAGAGAG "基序（DREME-5）。酵母一杂交试验和转录活性试验进一步证实了这一点。因此，MwMYB-1 激活了 AtMYB75 基因的表达，并对花青素的生物合成进行了级联放大。综上所述，我们的研究结果提供了对五倍子花青素生物合成调控的新认识，可用于促进树种农艺性状的改良。

{"title":"MYB-1 regulates anthocyanin biosynthesis in Magnolia wufengensis","authors":"Xian-ping Liu , Min Luo , Xiu-qi Liu , Liu-yan Hao , Chen Zhu , Li Wang , Lv-yi Ma","doi":"10.1016/j.plaphy.2024.109283","DOIUrl":"10.1016/j.plaphy.2024.109283","url":null,"abstract":"<div><div>Anthocyanin is an essential pigment in all major horticultural crops especially in ornamental trees. <em>Magnolia wufengensis</em> (new species of <em>Magnolia</em>) with red color flower was recently found as a popular species for ornamental use, but anthocyanin synthesis and regulation in <em>M</em>. <em>wufengensis</em> are poorly understood. Herein, transcriptome analysis was used to decipher the gene network associated with anthocyanin biosynthesis. An R2R3-like MwMYB-1 transcription factor was found. MwMYB-1 overexpression resulted in anthocyanin accumulation in tobacco and Arabidopsis. MwMYB-1 worked independently rather than forming a protein complex with bHLH or WD40 protein. According to MwMYB-1 DAP-seq analysis in Arabidopsis, the MwMYB-1 transcription factor preferred to bind the “AAGAGAG” motif (DREME-5) in the third exon of the <em>AtMYB75</em> gene. The yeast one hybrid assay and transcription activity assay further confirmed this. Thus, MwMYB-1 activated <em>AtMYB75</em> gene expression and conducted cascade amplification of anthocyanin biosynthesis. Taken together, our findings provide a novel understanding of anthocyanin biosynthesis regulation in <em>M. wufengensis</em> and can be used to promote agronomic trait improvement in tree species.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109283"},"PeriodicalIF":6.1,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide characterization of the DIR gene family in sesame reveals the function of SiDIR21 in lignan biosynthesis 芝麻中 DIR 基因家族的全基因组特征揭示了 SiDIR21 在木质素生物合成中的功能。

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2024-11-08 DOI: 10.1016/j.plaphy.2024.109282

Huan Li , Fengduo Hu , Jianglong Zhou, Li Yang, Donghua Li, Rong Zhou, Ting Zhou, Yanxin Zhang, Linhai Wang, Jun You

Furofuran-type lignans, mainly sesamin and sesamolin, are the most representative functional active ingredients in sesame (Sesamum indicum L.). Their exceptional antioxidant properties, medicinal benefits, and health-promoting functions have garnered significant attention. Dirigent (DIR) proteins, found in vascular plants, are crucial for the biosynthesis of secondary metabolites, like lignans, and essential for responding to abiotic and biotic stresses. Despite their importance, they have yet to be systematically analyzed, especially those involved in lignan synthesis in sesame. This study unveiled 44 DIR genes in sesame. Phylogenetic analysis categorized these SiDIRs into five subgroups (DIR-a, DIR-b/d, DIR-e, DIR-f, and DIR-g), aligning with conserved motifs and gene structures analyses. Expression analysis unveiled distinct tissue-specific and hormone-responsive expression patterns among the SiDIR gene family members. Particularly, SiDIR21, a member of the DIR-a subgroup, exhibited robust expression in lignan-accumulating tissues and consistently high expression levels in germplasm during seed development with high sesamin content. Furthermore, SiDIR21 overexpression in hairy roots significantly increased sesamin and sesamolin contents, confirming its role in lignan synthesis. Overall, our study offers a valuable resource for exploring SiDIRs’ functions and the lignan biosynthesis pathway in sesame.

呋喃类木酚素，主要是芝麻素和芝麻酚林，是芝麻（Sesamum indicum L.）中最具代表性的功能性活性成分。它们具有卓越的抗氧化性、药用价值和促进健康的功能，因此备受关注。存在于维管束植物中的Dirigent（DIR）蛋白对于木酚素等次生代谢物的生物合成至关重要，对于应对非生物和生物胁迫也必不可少。尽管它们非常重要，但尚未对其进行系统分析，尤其是那些参与芝麻木质素合成的基因。本研究揭示了芝麻中的 44 个 DIR 基因。系统发育分析将这些SiDIRs分为五个亚组（DIR-a、DIR-b/d、DIR-e、DIR-f和DIR-g），并与保守的主题和基因结构分析相一致。表达分析揭示了 SiDIR 基因家族成员之间不同的组织特异性和激素反应性表达模式。特别是 DIR-a 亚组成员 SiDIR21 在木质素积累组织中表现出强劲的表达，在芝麻素含量较高的种子发育过程中，其在种质中的表达水平一直很高。此外，SiDIR21 在毛根中的过表达显著增加了芝麻素和芝麻酚含量，证实了它在木质素合成中的作用。总之，我们的研究为探索 SiDIRs 的功能和芝麻木质素的生物合成途径提供了宝贵的资源。

{"title":"Genome-wide characterization of the DIR gene family in sesame reveals the function of SiDIR21 in lignan biosynthesis","authors":"Huan Li , Fengduo Hu , Jianglong Zhou, Li Yang, Donghua Li, Rong Zhou, Ting Zhou, Yanxin Zhang, Linhai Wang, Jun You","doi":"10.1016/j.plaphy.2024.109282","DOIUrl":"10.1016/j.plaphy.2024.109282","url":null,"abstract":"<div><div>Furofuran-type lignans, mainly sesamin and sesamolin, are the most representative functional active ingredients in sesame (<em>Sesamum indicum</em> L.). Their exceptional antioxidant properties, medicinal benefits, and health-promoting functions have garnered significant attention. Dirigent (DIR) proteins, found in vascular plants, are crucial for the biosynthesis of secondary metabolites, like lignans, and essential for responding to abiotic and biotic stresses. Despite their importance, they have yet to be systematically analyzed, especially those involved in lignan synthesis in sesame. This study unveiled 44 DIR genes in sesame. Phylogenetic analysis categorized these SiDIRs into five subgroups (DIR-a, DIR-b/d, DIR-e, DIR-f, and DIR-g), aligning with conserved motifs and gene structures analyses. Expression analysis unveiled distinct tissue-specific and hormone-responsive expression patterns among the SiDIR gene family members. Particularly, <em>SiDIR21</em>, a member of the DIR-a subgroup, exhibited robust expression in lignan-accumulating tissues and consistently high expression levels in germplasm during seed development with high sesamin content. Furthermore, <em>SiDIR21</em> overexpression in hairy roots significantly increased sesamin and sesamolin contents, confirming its role in lignan synthesis. Overall, our study offers a valuable resource for exploring SiDIRs’ functions and the lignan biosynthesis pathway in sesame.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109282"},"PeriodicalIF":6.1,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histone demethylase JMJ713 interaction with JMJ708 modulating H3K36me2, enhances rice heat tolerance through promoting hydrogen peroxide scavenging 组蛋白去甲基化酶 JMJ713 与调节 H3K36me2 的 JMJ708 相互作用，通过促进过氧化氢清除增强水稻的耐热性。

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2024-11-08 DOI: 10.1016/j.plaphy.2024.109284

Jiaxin Chai , Xiangyang Gu , Pengyu Song , Xinzhou Zhao , Yingjie Gao , Haiqi Wang , Qian Zhang , Tingting Cai , Yutong Liu , Xiaoting Li , Tao Song , Zhengge Zhu

The Earth is currently undergoing rapid warming cause of the accumulation in greenhouse gas emissions into the atmosphere and the consequent rise in global temperatures. High temperatures can bring the effects on rice development and growth and thereby decrease rice yield. In this study, we have identified that both JMJ713 and JMJ708 possess distinct histone demethylase activities. Specifically, JMJ713 modulates the levels of H3K36me2 while JMJ708 alters H3K9me3. Additionally, we have observed an interaction between JMJ713 and JMJ708, which collectively modify the level of H3K36me2. Furthermore, our findings demonstrate that JMJ713 plays an essential role to heat stress responses in rice (Oryza sativa). The overexpression of JMJ713 enhances heat tolerance in rice, whereas JMJ713 RNA interference rice lines exhibit increased sensitivity to heat. Further investigations revealed that overexpression of JMJ713 activated catalase (CAT) and peroxidase (POD) activities by mitigating excessive accumulation of reactive oxygen species (ROS) caused by heat stress. Interestingly, the setting rates of JMJ713 RNA interference lines decreased in comparing to wild-type, indicating that JMJ713 might play a crucial role in the rice seed development stage as well. Collectively, this study not only highlights JMJ713 is involved in heat stress responses but also provides insights into the conserved Fe(Ⅱ) and α-ketoglutarate (KG) binding residues are crucial for the demethylase activity of JMJ713, as well as JMJ713 interacts with JMJ708 to jointly regulate the levels of H3K36me2.

由于排放到大气中的温室气体不断积累，全球气温随之升高，目前地球正在迅速变暖。高温会影响水稻的生长发育，从而导致水稻减产。在这项研究中，我们发现 JMJ713 和 JMJ708 都具有不同的组蛋白去甲基化酶活性。具体来说，JMJ713 能调节 H3K36me2 的水平，而 JMJ708 则能改变 H3K9me3 的水平。此外，我们还观察到 JMJ713 和 JMJ708 之间的相互作用，它们共同改变了 H3K36me2 的水平。此外，我们的研究结果表明，JMJ713 对水稻（Oryza sativa）的热胁迫反应起着至关重要的作用。过表达 JMJ713 能增强水稻的耐热性，而 JMJ713 RNA 干扰水稻品系则表现出对热敏性的增加。进一步的研究发现，过表达 JMJ713 能缓解热胁迫引起的活性氧（ROS）过度积累，从而激活过氧化氢酶（CAT）和过氧化物酶（POD）的活性。有趣的是，与野生型相比，JMJ713 RNA 干扰株系的结实率降低，这表明 JMJ713 可能在水稻种子发育阶段也起着关键作用。综上所述，本研究不仅揭示了JMJ713参与了热胁迫响应，而且还揭示了保守的Fe（Ⅱ）和α-酮戊二酸（KG）结合残基对JMJ713的去甲基化酶活性至关重要，以及JMJ713与JMJ708相互作用共同调控H3K36me2的水平。

{"title":"Histone demethylase JMJ713 interaction with JMJ708 modulating H3K36me2, enhances rice heat tolerance through promoting hydrogen peroxide scavenging","authors":"Jiaxin Chai , Xiangyang Gu , Pengyu Song , Xinzhou Zhao , Yingjie Gao , Haiqi Wang , Qian Zhang , Tingting Cai , Yutong Liu , Xiaoting Li , Tao Song , Zhengge Zhu","doi":"10.1016/j.plaphy.2024.109284","DOIUrl":"10.1016/j.plaphy.2024.109284","url":null,"abstract":"<div><div>The Earth is currently undergoing rapid warming cause of the accumulation in greenhouse gas emissions into the atmosphere and the consequent rise in global temperatures. High temperatures can bring the effects on rice development and growth and thereby decrease rice yield. In this study, we have identified that both JMJ713 and JMJ708 possess distinct histone demethylase activities. Specifically, JMJ713 modulates the levels of H3K36me2 while JMJ708 alters H3K9me3. Additionally, we have observed an interaction between JMJ713 and JMJ708, which collectively modify the level of H3K36me2. Furthermore, our findings demonstrate that JMJ713 plays an essential role to heat stress responses in rice (<em>Oryza sativa</em>). The overexpression of <em>JMJ713</em> enhances heat tolerance in rice, whereas <em>JMJ7</em>13 RNA interference rice lines exhibit increased sensitivity to heat. Further investigations revealed that overexpression of <em>JMJ713</em> activated catalase (CAT) and peroxidase (POD) activities by mitigating excessive accumulation of reactive oxygen species (ROS) caused by heat stress. Interestingly, the setting rates of <em>JMJ7</em>13 RNA interference lines decreased in comparing to wild-type, indicating that <em>JMJ7</em>13 might play a crucial role in the rice seed development stage as well. Collectively, this study not only highlights JMJ713 is involved in heat stress responses but also provides insights into the conserved Fe(Ⅱ) and α-ketoglutarate (KG) binding residues are crucial for the demethylase activity of JMJ713, as well as JMJ713 interacts with JMJ708 to jointly regulate the levels of H3K36me2.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109284"},"PeriodicalIF":6.1,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pseudomonas putida triggers phosphorus bioavailability and P-transporters under different phosphate regimes to enhance maize growth 假单胞菌（Pseudomonas putida）在不同磷酸盐制度下触发磷生物利用率和磷转运体，以促进玉米生长。

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2024-11-07 DOI: 10.1016/j.plaphy.2024.109279

Tanya Singh , Nikita Bisht , Mohd Mogees Ansari , Puneet Singh Chauhan

The decline of available phosphorus in soil due to anthropogenic activities necessitates utilizing soil microorganisms that influence soil phosphorus levels. However, the specific mechanisms governing their interaction in Zea mays under diverse phosphate regimes remain largely unknown. The present study investigated the dynamics of phosphorus solubilization and the impact of organic acid supplementation in combination with the beneficial rhizobacterium Pseudomonas putida (RA) on maize growth under phosphorus-limiting and unavailable conditions. HPLC analysis revealed gluconic acid as the primary organic acid (OA) produced by P. putida across all three conditions (P-sufficient, P-limiting, and P-unavailable), with the highest production occurring under P-limiting conditions. The study evaluates the effects of RA, OA, and OA + RA on plant growth parameters under P-limiting and insufficient conditions, revealing significant alterations in growth and biochemical parameters (P = 0.05) compared to their respective untreated controls. Additionally, plants treated with organic acids and bacterial inoculation show increased phosphorus concentrations in both roots and shoots. Gene expression analysis of key phosphorus transporter genes (PHT1, PHO1, PTF, PHF1) further supports the role of organic acids and bacterial inoculation in enhancing phosphorus uptake. In conclusion, our study affirms that the secretion of gluconic acid by RA and its plant growth-promoting properties boost phosphorus uptake and maize growth by increasing phosphorus availability and influencing the expression of phosphorus transport-related genes.

人为活动导致土壤中可利用的磷减少，因此有必要利用影响土壤磷含量的土壤微生物。然而，在不同的磷酸盐制度下，玉米中微生物相互作用的具体机制在很大程度上仍不为人所知。本研究调查了磷溶解的动态以及有机酸补充与有益根瘤菌假单胞菌（RA）相结合对限磷和缺磷条件下玉米生长的影响。高效液相色谱分析显示，葡萄糖酸是腐生假单胞菌在所有三种条件（磷充足、磷限制和磷不可得）下产生的主要有机酸（OA），其中在磷限制条件下产量最高。研究评估了 RA、OA 和 OA + RA 在 P 限制和 P 不足条件下对植物生长参数的影响，结果显示，与各自未处理的对照组相比，植物的生长和生化参数发生了显著变化（P = 0.05）。此外，经有机酸和细菌接种处理的植物根部和芽中的磷浓度都有所增加。关键磷转运基因（PHT1、PHO1、PTF、PHF1）的基因表达分析进一步证实了有机酸和细菌接种在提高磷吸收方面的作用。总之，我们的研究证实，RA 分泌的葡萄糖酸及其促进植物生长的特性通过增加磷的可用性和影响磷转运相关基因的表达，促进了磷的吸收和玉米的生长。

{"title":"Pseudomonas putida triggers phosphorus bioavailability and P-transporters under different phosphate regimes to enhance maize growth","authors":"Tanya Singh , Nikita Bisht , Mohd Mogees Ansari , Puneet Singh Chauhan","doi":"10.1016/j.plaphy.2024.109279","DOIUrl":"10.1016/j.plaphy.2024.109279","url":null,"abstract":"<div><div>The decline of available phosphorus in soil due to anthropogenic activities necessitates utilizing soil microorganisms that influence soil phosphorus levels. However, the specific mechanisms governing their interaction in <em>Zea mays</em> under diverse phosphate regimes remain largely unknown. The present study investigated the dynamics of phosphorus solubilization and the impact of organic acid supplementation in combination with the beneficial rhizobacterium <em>Pseudomonas putida</em> (RA) on maize growth under phosphorus-limiting and unavailable conditions. HPLC analysis revealed gluconic acid as the primary organic acid (OA) produced by <em>P. putida</em> across all three conditions (P-sufficient, P-limiting, and P-unavailable), with the highest production occurring under P-limiting conditions. The study evaluates the effects of RA, OA, and OA + RA on plant growth parameters under P-limiting and insufficient conditions, revealing significant alterations in growth and biochemical parameters (<em>P =</em> 0.05) compared to their respective untreated controls. Additionally, plants treated with organic acids and bacterial inoculation show increased phosphorus concentrations in both roots and shoots. Gene expression analysis of key phosphorus transporter genes (<em>PHT1, PHO1, PTF, PHF1</em>) further supports the role of organic acids and bacterial inoculation in enhancing phosphorus uptake. In conclusion, our study affirms that the secretion of gluconic acid by RA and its plant growth-promoting properties boost phosphorus uptake and maize growth by increasing phosphorus availability and influencing the expression of phosphorus transport-related genes.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109279"},"PeriodicalIF":6.1,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the synergistic effects of nano-zinc and biochar in mitigating aluminum toxicity in soybeans 研究纳米锌和生物炭在减轻大豆铝毒性方面的协同效应。

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2024-11-07 DOI: 10.1016/j.plaphy.2024.109275

Enxi Zhang , Kun Liu , Suwen Liang , Lingrui Liu , Hai Nian , Tengxiang Lian

Aluminum (Al) toxicity limited root growth by reducing nutrient translocation and promoting reactive oxygen species (ROS) accumulation, particularly in soybean. The endophyte of root could be modified by plant metabolites, which could potentially alter the tolerance to environmental toxicity of plants in acidic-Al soils. To explore how they help soybean mitigate Al toxicity by altering root endophytes, zinc oxide nanoparticles (ZnO NPs) at doses of 0, 30, 60, 90 mg/kg and 2% biochar (BC) were selected as bio modifiers, and Al₂(SO₄)₃ at 19 mg/kg was used to simulate Al toxicity. We analyzed root endophytes and metabolites by high-throughput sequencing and gas chromatography-mass spectrometry (GC-MS). We found that ZnO NPs with BC could bolster soybean resilience against Al toxicity by enriching soil nutrients, activating enzymes, and bolstering antioxidant mechanisms. We also observed that it enriched root endophytic microbial diversity, notably increasing populations of Nakamurella, Aureimonas, Luteimonas, and Sphingomonas. These changes in the endophytes contributed to the improved adaptability of plants to adversity under Al toxicity. This study highlighted the potential of using ZnO NPs and BC as a sustainable approach to combat Al toxicity, emphasizing the intricate interplay between plant physiology and rhizosphere microbial dynamics in mitigating the effects of environmental toxicity.

铝（Al）毒性通过减少养分转运和促进活性氧（ROS）积累限制根系生长，尤其是在大豆中。根部的内生菌可被植物代谢产物改变，这有可能改变酸性铝土壤中植物对环境毒性的耐受性。为了探索如何通过改变根部内生菌来帮助大豆减轻铝毒性，我们选择了剂量为 0、30、60、90 毫克/千克的氧化锌纳米颗粒（ZnO NPs）和 2% 的生物炭（BC）作为生物调节剂，并使用 19 毫克/千克的 Al2(SO4)3 来模拟铝毒性。我们通过高通量测序和气相色谱-质谱（GC-MS）分析了根系内生菌和代谢物。我们发现，含有 BC 的 ZnO NPs 可通过富集土壤养分、激活酶和加强抗氧化机制来增强大豆对铝毒性的抗性。我们还观察到，它丰富了根部内生微生物的多样性，特别是增加了 Nakamurella、Aureimonas、Luteimonas 和 Sphingomonas 的数量。内生微生物的这些变化有助于提高植物对铝毒性逆境的适应能力。这项研究强调了使用氧化锌氮氧化物和碱性生物碱作为一种可持续方法来对抗铝毒性的潜力，同时强调了植物生理机能和根圈微生物动态之间在减轻环境毒性影响方面错综复杂的相互作用。

{"title":"Investigating the synergistic effects of nano-zinc and biochar in mitigating aluminum toxicity in soybeans","authors":"Enxi Zhang , Kun Liu , Suwen Liang , Lingrui Liu , Hai Nian , Tengxiang Lian","doi":"10.1016/j.plaphy.2024.109275","DOIUrl":"10.1016/j.plaphy.2024.109275","url":null,"abstract":"<div><div>Aluminum (Al) toxicity limited root growth by reducing nutrient translocation and promoting reactive oxygen species (ROS) accumulation, particularly in soybean. The endophyte of root could be modified by plant metabolites, which could potentially alter the tolerance to environmental toxicity of plants in acidic-Al soils. To explore how they help soybean mitigate Al toxicity by altering root endophytes, zinc oxide nanoparticles (ZnO NPs) at doses of 0, 30, 60, 90 mg/kg and 2% biochar (BC) were selected as bio modifiers, and Al<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub> at 19 mg/kg was used to simulate Al toxicity. We analyzed root endophytes and metabolites by high-throughput sequencing and gas chromatography-mass spectrometry (GC-MS). We found that ZnO NPs with BC could bolster soybean resilience against Al toxicity by enriching soil nutrients, activating enzymes, and bolstering antioxidant mechanisms. We also observed that it enriched root endophytic microbial diversity, notably increasing populations of <em>Nakamurella</em>, <em>Aureimonas</em>, <em>Luteimonas</em>, and <em>Sphingomonas</em>. These changes in the endophytes contributed to the improved adaptability of plants to adversity under Al toxicity. This study highlighted the potential of using ZnO NPs and BC as a sustainable approach to combat Al toxicity, emphasizing the intricate interplay between plant physiology and rhizosphere microbial dynamics in mitigating the effects of environmental toxicity.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109275"},"PeriodicalIF":6.1,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Grapevine and maize: Two guard cell shaped strategies to cope with repeated drought stress 葡萄和玉米：应对反复干旱胁迫的两种护卫细胞形态策略

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2024-11-07 DOI: 10.1016/j.plaphy.2024.109262

Patrick Pascal Lehr , Alexander Erban , Roman Paul Hartwig , Monika Andrea Wimmer , Joachim Kopka , Christian Zörb

Adaptation of crops to recurrent drought stress is crucial for maintaining agricultural productivity and achieving food security under changing climate. Guard cells, pivotal regulators of plant water usage and assimilation, are central to this adaptation process. However, the metabolic dynamics of guard cells under drought stress remain poorly understood, particularly in grapevine, a prominent crop grown in arid regions, and maize, a staple crop with substantial water requirements. In this study, differences in guard cells metabolism during drought stress of grapevine and maize were investigated by performing physiological and metabolomic analyses. Metabolomic analysis highlighted differential responses in amino acids and sugars, with grapevine guard cells displaying greater stability in amino acid and sugar signatures, while maize showed marked increases in sugar levels. These findings suggest two distinct adaptive strategies, a vigorous acclimation of guard cells, as observed in maize, and an attenuated acclimation of guard cells, shown in grapevine. Understanding these metabolic adjustments is helpful for enhancing drought resilience in agricultural systems.

在不断变化的气候条件下，作物对经常性干旱胁迫的适应对于保持农业生产力和实现粮食安全至关重要。守护细胞是植物水分利用和同化的关键调节器，是这一适应过程的核心。然而，人们对干旱胁迫下保卫细胞的新陈代谢动态仍然知之甚少，尤其是在干旱地区种植的主要作物葡萄和需要大量水分的主要作物玉米中。本研究通过生理和代谢组学分析，研究了葡萄和玉米在干旱胁迫下保卫细胞代谢的差异。代谢组分析强调了氨基酸和糖类的不同反应，葡萄的保卫细胞显示出更稳定的氨基酸和糖类特征，而玉米则显示出糖类水平的明显增加。这些研究结果表明了两种不同的适应策略，一种是在玉米中观察到的防护细胞的强烈适应，另一种是在葡萄中观察到的防护细胞的减弱适应。了解这些代谢调整有助于提高农业系统的抗旱能力。

{"title":"Grapevine and maize: Two guard cell shaped strategies to cope with repeated drought stress","authors":"Patrick Pascal Lehr , Alexander Erban , Roman Paul Hartwig , Monika Andrea Wimmer , Joachim Kopka , Christian Zörb","doi":"10.1016/j.plaphy.2024.109262","DOIUrl":"10.1016/j.plaphy.2024.109262","url":null,"abstract":"<div><div>Adaptation of crops to recurrent drought stress is crucial for maintaining agricultural productivity and achieving food security under changing climate. Guard cells, pivotal regulators of plant water usage and assimilation, are central to this adaptation process. However, the metabolic dynamics of guard cells under drought stress remain poorly understood, particularly in grapevine, a prominent crop grown in arid regions, and maize, a staple crop with substantial water requirements. In this study, differences in guard cells metabolism during drought stress of grapevine and maize were investigated by performing physiological and metabolomic analyses. Metabolomic analysis highlighted differential responses in amino acids and sugars, with grapevine guard cells displaying greater stability in amino acid and sugar signatures, while maize showed marked increases in sugar levels. These findings suggest two distinct adaptive strategies, a vigorous acclimation of guard cells, as observed in maize, and an attenuated acclimation of guard cells, shown in grapevine. Understanding these metabolic adjustments is helpful for enhancing drought resilience in agricultural systems.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109262"},"PeriodicalIF":6.1,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The changes in sugar content and the selection of key genes at different developmental stages of 'Katy' and 'Kuijin' apricots Katy "和 "Kuijin "杏不同发育阶段含糖量的变化和关键基因的选择。

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2024-11-07 DOI: 10.1016/j.plaphy.2024.109280

Xueping Han , Caina Jiang , GuipingWang , Jinzheng Wang , Peixian Nie , Xiaomin Xue

'Katy' and 'Kuijin' apricots are the main cultivated varieties in Shandong province. The flavor of the fruit is mainly determined by sugars and acids, with soluble sugar components serving as important nutritional elements in fruits as well as crucial indicators of fruit sweetness and flavor quality. However, little is known about the changes in soluble sugar content, especially sucrose content, and the sucrose metabolism mechanism during the entire fruit growth and development process of 'Katy' and 'Kuijin' apricots. In this study, we first detected the changes in sucrose, fructose, and glucose content at nine fruit development stages of 'Katy' and 'Kuijin' apricots, and found that the stage of rapid accumulation of sucrose and fructose was from 56 days after full bloom (DAF) to 63 DAF. Therefore, we identified the key gene PaSS1 of sucrose synthase through transcriptome data screening, and further analyzed the function of the PaSS1 gene in fruit sucrose metabolism process using virus-induced gene silencing (VIGS) technology. Silencing the PaSS1 gene reduced the breakdown activity of sucrose synthase, increasing sucrose content while decreasing glucose and fructose content, delaying fruit coloring and ripening, indicating that the PaSS1 gene may regulate the ripening of apricot fruits. This study provides a theoretical basis for further research on the molecular mechanism of the PaSS1 gene in apricot fruit ripening process.

凯蒂 "和 "魁金 "杏是山东省的主要栽培品种。果实的风味主要由糖和酸决定，其中可溶性糖成分是果实的重要营养成分，也是果实甜度和风味品质的重要指标。然而，人们对 "凯蒂 "杏和 "夔金 "杏在整个果实生长发育过程中可溶性糖含量（尤其是蔗糖含量）的变化以及蔗糖代谢机制知之甚少。本研究首先检测了'Katy'和'Kuijin'杏果实发育九个阶段中蔗糖、果糖和葡萄糖含量的变化，发现蔗糖和果糖的快速积累阶段为盛花后 56 天至 63 天。因此，我们通过转录组数据筛选确定了蔗糖合成酶的关键基因 PaSS1，并利用病毒诱导基因沉默（VIGS）技术进一步分析了 PaSS1 基因在果实蔗糖代谢过程中的功能。Silencing the PaSS1 gene reduced the breakdown activity of sucrose synthase, increasing sucrose content while lower glucose and fructose content, delaying fruit coloring and ripeing, indicating that the PaSS1 gene may regulate the ripeing of apricot fruit.该研究为进一步研究 PaSS1 基因在杏果实成熟过程中的分子机理提供了理论依据。

{"title":"The changes in sugar content and the selection of key genes at different developmental stages of 'Katy' and 'Kuijin' apricots","authors":"Xueping Han , Caina Jiang , GuipingWang , Jinzheng Wang , Peixian Nie , Xiaomin Xue","doi":"10.1016/j.plaphy.2024.109280","DOIUrl":"10.1016/j.plaphy.2024.109280","url":null,"abstract":"<div><div>'Katy' and 'Kuijin' apricots are the main cultivated varieties in Shandong province. The flavor of the fruit is mainly determined by sugars and acids, with soluble sugar components serving as important nutritional elements in fruits as well as crucial indicators of fruit sweetness and flavor quality. However, little is known about the changes in soluble sugar content, especially sucrose content, and the sucrose metabolism mechanism during the entire fruit growth and development process of 'Katy' and 'Kuijin' apricots. In this study, we first detected the changes in sucrose, fructose, and glucose content at nine fruit development stages of 'Katy' and 'Kuijin' apricots, and found that the stage of rapid accumulation of sucrose and fructose was from 56 days after full bloom (DAF) to 63 DAF. Therefore, we identified the key gene <em>PaSS1</em> of sucrose synthase through transcriptome data screening, and further analyzed the function of the <em>PaSS1</em> gene in fruit sucrose metabolism process using virus-induced gene silencing (VIGS) technology. Silencing the <em>PaSS1</em> gene reduced the breakdown activity of sucrose synthase, increasing sucrose content while decreasing glucose and fructose content, delaying fruit coloring and ripening, indicating that the <em>PaSS1</em> gene may regulate the ripening of apricot fruits. This study provides a theoretical basis for further research on the molecular mechanism of the <em>PaSS1</em> gene in apricot fruit ripening process.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109280"},"PeriodicalIF":6.1,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiomics analysis reveals the involvement of OnDIVARICATA 3 in controlling dynamic flower coloring of Oncidium hybridum 多组学分析揭示了 OnDIVARICATA 3 参与控制 Oncidium hybridum 的动态花色。

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry

Pub Date : 2024-11-07 DOI: 10.1016/j.plaphy.2024.109277

Yuanhua Luo , Yan Chen , Nengyan Fang, Lan Kong, Rongyan Lin, Yiquan Chen, Ronghui Fan, Huaiqin Zhong, Minling Huang, Xiuxian Ye

Flower color is one of the main quality and economic traits of ornamental plants, and a large amount of research on flower color mainly focuses on the differences between varieties, while there were few reports on the change of flower color at different developmental stages. In this study, the metabolome and transcriptome of a new strain ‘XM-1’ with dynamic color changes of Oncidium were analyzed. The results showed that rutin, quercetin and carotenoids metabolism decreased significantly during the change of color from yellow to white. Analyzing the correlation network between metabolites and differential expressed genes, 25 key structural genes were detected and regulated by multiple MYB-related transcription factors. The MYB-related transcription factor Cluster-100966.1_OnDIVARICATA 3 was selected for further analysis. The phylogenetic tree of DIVARICATA in different species of Orchidaceae and Arabidopsis thaliana was constructed and the most closely related members were selected for sequence comparison. The results showed that OnDIVARICATA 3 contained MYB-like conserved domains. Subcellular localization results showed that OnDIVARICATA 3 was located in the nucleus. In overexpressing OnDIVARICATA 3 transgenic hairy roots, the expression of flower color related genes FLS, ZEP, and CHYB were significantly up-regulated. In summary, this study characterized the key metabolic pathways in the formation of the dynamic flower color of Oncidium hybridum, and constructed the regulatory network of the MYB-related. These results laid a theoretical foundation for the subsequent research on flower color and genetic engineering technology breeding of Oncidium hybridum.

花色是观赏植物的主要品质和经济性状之一，大量关于花色的研究主要集中在品种间的差异上，而关于不同发育阶段花色变化的报道较少。本研究分析了具有动态花色变化的新菌株 "XM-1 "的代谢组和转录组。结果表明，芦丁、槲皮素和类胡萝卜素的代谢在花色由黄色变为白色的过程中显著下降。通过分析代谢物与差异表达基因之间的相关网络，发现25个关键结构基因受多个MYB相关转录因子调控。选择 MYB 相关转录因子 Cluster-100966.1_OnDIVARICATA 3 作进一步分析。构建了兰科不同物种和拟南芥中 DIVARICATA 的系统发生树，并选择关系最密切的成员进行序列比较。结果表明，OnDIVARICATA 3含有类似MYB的保守结构域。亚细胞定位结果显示，OnDIVARICATA 3 位于细胞核中。在过表达 OnDIVARICATA 3 的转基因毛根中，花色相关基因 FLS、ZEP 和 CHYB 的表达显著上调。综上所述，本研究表征了杂交大花蕙兰动态花色形成的关键代谢途径，并构建了 MYB 相关的调控网络。这些结果为杂交鬼针草后续的花色研究和基因工程技术育种奠定了理论基础。

{"title":"Multiomics analysis reveals the involvement of OnDIVARICATA 3 in controlling dynamic flower coloring of Oncidium hybridum","authors":"Yuanhua Luo , Yan Chen , Nengyan Fang, Lan Kong, Rongyan Lin, Yiquan Chen, Ronghui Fan, Huaiqin Zhong, Minling Huang, Xiuxian Ye","doi":"10.1016/j.plaphy.2024.109277","DOIUrl":"10.1016/j.plaphy.2024.109277","url":null,"abstract":"<div><div>Flower color is one of the main quality and economic traits of ornamental plants, and a large amount of research on flower color mainly focuses on the differences between varieties, while there were few reports on the change of flower color at different developmental stages. In this study, the metabolome and transcriptome of a new strain ‘XM-1’ with dynamic color changes of <em>Oncidium</em> were analyzed. The results showed that rutin, quercetin and carotenoids metabolism decreased significantly during the change of color from yellow to white. Analyzing the correlation network between metabolites and differential expressed genes, 25 key structural genes were detected and regulated by multiple MYB-related transcription factors. The MYB-related transcription factor <em>Cluster-100966.1_OnDIVARICATA 3</em> was selected for further analysis. The phylogenetic tree of DIVARICATA in different species of Orchidaceae and <em>Arabidopsis thaliana</em> was constructed and the most closely related members were selected for sequence comparison. The results showed that OnDIVARICATA 3 contained MYB-like conserved domains. Subcellular localization results showed that OnDIVARICATA 3 was located in the nucleus. In overexpressing <em>OnDIVARICATA 3</em> transgenic hairy roots, the expression of flower color related genes <em>FLS</em>, <em>ZEP</em>, and <em>CHYB</em> were significantly up-regulated. In summary, this study characterized the key metabolic pathways in the formation of the dynamic flower color of <em>Oncidium hybridum</em>, and constructed the regulatory network of the MYB-related. These results laid a theoretical foundation for the subsequent research on flower color and genetic engineering technology breeding of <em>Oncidium hybridum</em>.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"217 ","pages":"Article 109277"},"PeriodicalIF":6.1,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142626624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0