Postharvest Biology and Technology最新文献_第9页

Storage temperature affects metabolism of sweet corn 储藏温度影响甜玉米的新陈代谢

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2024-09-26 DOI: 10.1016/j.postharvbio.2024.113232

Shiyu Liu , Xinyuan Zhou , Yunxiang Wang , Qing Wang , Lili Ma , Cai’e Wu , Ronghuan Wang , Yaxing Shi , Christopher B. Watkins , Jinhua Zuo , Yanyan Zheng

Temperature is one of the critical factors affecting the postharvest storage quality of sweet corn, but effects of storage temperatures have not been elucidated at the molecular level. In this study, the effects of storage at 0 °C, 4 °C and 20 °C, on quality and associated effects on the transcriptome and metabolome were investigated. Compared with storage at 20 °C, cobs stored at 0 °C and 4 °C had up-regulated expression of genes associated with cell wall disassembly (PG, PE, BMY, β-Gal) and down-regulated expression of genes involved in lignin synthesis, the up-regulation of SPP, SPS and SUS involved in sucrose synthesis, and the down-regulation of IN, FK, HXK and PFK involved in sucrose catabolism. Most of the genes involved in ethylene biosynthesis and signaling were inhibited at 0 °C, except for ERFs, while abscisic acid synthesis and signal transduction were most active at this temperature. Near-freezing temperature also maintains the integrity of the cell membrane by inhibiting the expression of PLD, PLC and LOX, which encode membrane lipid-degrading enzymes. Metabolomics and combined analysis showed that the contents of lysophospholipid, glutathione and L-ascorbic acid in sweet corn increased at low temperatures. These results provide insight into the effects of low temperatures on sweet corn quality.

温度是影响甜玉米收获后贮藏质量的关键因素之一，但贮藏温度的影响尚未在分子水平上得到阐明。本研究调查了 0 ℃、4 ℃ 和 20 ℃ 储藏对质量的影响以及对转录组和代谢组的相关影响。与 20 °C贮藏相比，0 °C和 4 °C贮藏的玉米棒与细胞壁分解有关的基因（PG、PE、BMY、β-Gal）表达上调，与木质素合成有关的基因表达下调，与蔗糖合成有关的 SPP、SPS 和 SUS 基因上调，与蔗糖分解有关的 IN、FK、HXK 和 PFK 基因下调。在 0 °C 温度下，除 ERFs 外，大多数参与乙烯生物合成和信号转导的基因都受到抑制，而赤霉酸合成和信号转导在该温度下最为活跃。近冻结温度还能抑制编码膜脂降解酶的 PLD、PLC 和 LOX 的表达，从而保持细胞膜的完整性。代谢组学和综合分析表明，在低温条件下，甜玉米中溶血磷脂、谷胱甘肽和 L-抗坏血酸的含量增加。这些结果有助于深入了解低温对甜玉米品质的影响。

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引用次数: 0

Ursolic acid, a natural endogenous compound, inhibits browning in fresh-cut apples 熊果酸是一种天然内源性化合物，可抑制鲜切苹果的褐变

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2024-09-26 DOI: 10.1016/j.postharvbio.2024.113228

Song Zhang , Shuwen Wang , Yu Li, Jinjie Wang, Jingying Shi, Yong Peng, Pei Liu

Enzymatic browning occurs rapidly in fresh-cut apples and can lead to economic losses. Although various methods have been developed to prevent this browning, physical methods are costly, and chemical methods are controversial, suggesting the potential usefulness of an endogenous natural browning inhibitor. In this study, the effects of ursolic acid (UA), a natural endogenous compound, on apple browning were investigated. It was found that UA inhibited the activity of polyphenol oxidase through Cu²⁺ chelation. Molecular docking indicated that the inhibition was reversible and mixed-type. Moreover, UA treatment promoted the scavenging of reactive oxygen species in fresh-cut apple and reduced the proliferation of microorganisms on the apple surface. Similar effects were observed on the browning of chestnut, eggplant, potatoes, and pears. In conclusion, the findings demonstrate the efficacy of UA on inhibiting browning in fresh-cut apple, together with an analysis of the mechanism involved.

鲜切苹果会迅速发生酶促褐变，从而导致经济损失。虽然已经开发出多种方法来防止这种褐变，但物理方法成本高昂，化学方法也存在争议，这表明内源性天然褐变抑制剂具有潜在的作用。本研究调查了天然内源化合物熊果酸（UA）对苹果褐变的影响。研究发现，熊果酸通过 Cu2+ 螯合作用抑制了多酚氧化酶的活性。分子对接表明，这种抑制作用是可逆的、混合型的。此外，UA 处理可促进鲜切苹果中活性氧的清除，并减少苹果表面微生物的增殖。在栗子、茄子、马铃薯和梨的褐变过程中也观察到了类似的效果。总之，研究结果表明了 UA 在抑制鲜切苹果褐变方面的功效，并对其中的机理进行了分析。

引用次数: 0

Identification and characterization of terpene synthase genes accounting for volatile terpene emissions in the flower of Paeonia lactiflora 芍药花中挥发性萜烯合成酶基因的鉴定和特征描述

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2024-09-26 DOI: 10.1016/j.postharvbio.2024.113231

Qian Zhao , Yuqing Li , Lina Gu , Shuting Li , Di He , Shanrong Dong , Qingyu Zhang , Jianrang Luo , Yanlong Zhang

Emergence of floral scents marked a significant milestone in biological development of plant, letting out olfactory signals that enable them to induce pollinators. In this research, molecular and metabolomics methodologies were used to explore terpene synthase (TPS) genes, which are responsible for synthesis of floral fragrance in Paeonia lactiflora ‘Wu Hua Long Yu’. Analyses on transcriptome data identified 11 PlTPS genes, among them 7 genes were isolated, and the functions of 5 genes were confirmed. PlTPS1 was identified as a single-product enzyme; PlTPS4, 8 and 9 were identified as bifunctional enzymes, capable of recognizing farnesyl pyrophosphate and geranyl pyrophosphate; PlTPS6 generated multiple products by recognizing farnesyl pyrophosphate. Enzymatic products of these PlTPS proteins closely matched the volatile terpenes emitted by flowers, revealing the relation between PlTPS genes and the release of such volatile compounds. Functions of five PlTPS genes in herbaceous peony were further elucidated through virus-induced gene silencing experiments, as well as overexpression in tobacco. PlTPS1 catalyzes geraniol production, while PlTPS4 catalyzes linalool. PlTPS6 is responsible for producing two sesquiterpenes, caryophyllene and α-humulene. PlTPS8, with the most varied products, mainly catalyzes the production of α-pinene, linalool, and germacrene D. Based on these findings, graphical models summarizing the biosynthesis of floral volatile terpenes in herbaceous peony have been proposed. The characterization of PlTPS genes has elucidated their specific roles in the synthesis of herbaceous peony fragrance, opening up possibilities for introducing or enhancing floral scents in herbaceous peony and other plants.

花香的出现标志着植物生物学发展的一个重要里程碑，它发出的嗅觉信号使植物能够诱导传粉昆虫。本研究采用分子和代谢组学方法探讨了芍药'吴化龙玉'中负责合成花香的萜烯合成酶（TPS）基因。对转录组数据的分析发现了 11 个 PlTPS 基因，其中 7 个基因被分离出来，5 个基因的功能得到确认。PlTPS1被鉴定为单产物酶；PlTPS4、8和9被鉴定为双功能酶，能够识别焦磷酸芳萘酯和焦磷酸香叶酯；PlTPS6通过识别焦磷酸芳萘酯产生多种产物。这些 PlTPS 蛋白的酶产物与花朵释放的挥发性萜类化合物非常吻合，揭示了 PlTPS 基因与此类挥发性化合物释放之间的关系。通过病毒诱导的基因沉默实验以及在烟草中的过表达，进一步阐明了草本牡丹中五个 PlTPS 基因的功能。PlTPS1 催化香叶醇的产生，PlTPS4 催化芳樟醇的产生。PlTPS6 负责生产两种倍半萜类化合物，即叶绿素和 α-胡麻烯。PlTPS8 的产物种类最多，主要催化 α-蒎烯、芳樟醇和胚芽烯 D 的生成。根据这些发现，提出了草本牡丹花卉挥发性萜烯生物合成的图解模型。PlTPS 基因的特征阐明了它们在草本牡丹香味合成中的特殊作用，为在草本牡丹和其他植物中引入或增强花香提供了可能性。

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引用次数: 0

Development of a unified framework of low-rank approximation and deep neural networks for predicting the spatial variability of SSC in `Spania' watermelons using vis/NIR hyperspectral imaging 利用可见光/近红外高光谱成像技术，开发用于预测 "斯帕尼亚 "西瓜 SSC 空间变化的低秩近似和深度神经网络统一框架

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2024-09-25 DOI: 10.1016/j.postharvbio.2024.113222

Jobin Francis , Sony George , Binu M. Devassy , Sudhish N. George

Soluble Solids Content (SSC) is an important quality attribute that represents the internal quality of fruits. Visible and near-infrared (Vis/NIR) combined with chemometric algorithms are now popular methods for non-invasive measurement and visualization of SSC. However, in fruits with a thick rind and a large flesh volume, such as watermelon, SSC is not evenly distributed across the fruit. The variability in SSC across the fruit flesh may have an adverse effect on the accuracy of quality analysis algorithms. Thus, this paper presents an accurate and efficient approach for predicting the spatial variation of SSC in watermelons using a combined framework of low-rank approximation and deep neural networks. Watermelon ‘Spania’ was selected for this study, and hyperspectral images of each watermelon were taken from various views, including the top, bottom, and two lateral views. The low-rank property is employed as a constraint in this approach to eliminate the unwanted variations in the spectral data. The low-rank component of the spectral data, free of unwanted variations, is then fed into a fully connected neural network (FNN) for the prediction of watermelon SSC values. The proposed approach obtained optimal performance in calibration and prediction with

R_{C}^{2} = 0.982

, RMSEP = 0.132, and

R_{P}^{2} = 0.945

, RMSEP = 0.195 respectively. Further, the prediction outcomes of the proposed method were compared with state-of-the-art such as Partial Least Square Regression (PLSR), Support Vector Regression (SVR), Multiple Linear Regression (MLR), Decision Tree (DT), and Random Forest (RF). The overall results showed that combining HSI data with a low-rank and deep neural network framework is an efficient method for accurately predicting SSC in watermelons as well as correctly predicting spatial variability of SSC in individual fruits.

可溶性固形物含量（SSC）是代表水果内部质量的重要质量属性。可见光和近红外（可见光/近红外）结合化学计量学算法是目前非侵入式测量和可视化 SSC 的流行方法。然而，在西瓜等果皮厚、果肉体积大的水果中，SSC 在整个水果中的分布并不均匀。果肉中 SSC 的变化可能会对质量分析算法的准确性产生不利影响。因此，本文提出了一种准确、高效的方法，利用低秩近似和深度神经网络的组合框架来预测西瓜 SSC 的空间变化。本研究选择了西瓜 "Spania"，并从不同视角（包括顶部、底部和两个侧视图）拍摄了每个西瓜的高光谱图像。这种方法采用低秩属性作为约束，以消除光谱数据中不必要的变化。然后，将光谱数据中没有多余变化的低秩分量输入全连接神经网络（FNN），用于预测西瓜的 SSC 值。所提出的方法在校准和预测方面获得了最佳性能，RC2=0.982，RMSEP=0.132；RP2=0.945，RMSEP=0.195。此外，还将所提方法的预测结果与最先进的方法进行了比较，如部分最小平方回归（PLSR）、支持向量回归（SVR）、多元线性回归（MLR）、决策树（DT）和随机森林（RF）。总体结果表明，将 HSI 数据与低秩深度神经网络框架相结合是准确预测西瓜 SSC 以及正确预测单个果实 SSC 空间变化的有效方法。

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引用次数: 0

Molecular and metabolic insights into the mechanism of exogenous methyl jasmonate in enhancing the postharvest resistance of kiwifruit to Botrytis cinerea 外源茉莉酸甲酯增强猕猴桃采后对灰葡萄孢菌抗性的分子和代谢机理探究

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2024-09-25 DOI: 10.1016/j.postharvbio.2024.113226

Jiaqi Yang , Yijia Ma , Tianjing Zeng, Zhexin Li, Yuan Sui, Wenlin Zhang, Hongpan Zhong, Xu Wang

Gray mold, caused by Botrytis cinerea infection, significantly impacts postharvest kiwifruit, leading to spoilage and food safety issues. Meanwhile, methyl jasmonate (MeJA) induces defense responses in plants. This study aimed to identify the optimal MeJA concentration to induce disease resistance in kiwifruits. Notably, various plant defense enzymes were detected in MeJA-treated kiwifruit. Moreover, 0.01 mol/L MeJA was found to enhance B. cinerea resistance in kiwifruit by increasing antioxidant enzyme activity. Widely targeted metabolomics analysis revealed 62–64 differentially expressed metabolites (DEMs) between the different treatment groups. Additionally, RNA-seq analysis revealed 930–2900 differentially expressed genes (DEGs), between these groups. Subsequently, combined DEG and DEM analysis highlighted phenylpropanoid biosynthesis and plant hormone signaling as key transduction pathway associated with MeJA-induced resistance. Overall, these findings identified the mechanism of MeJA-induced resistance in kiwifruit, providing a theoretical basis for the safe and effective control of postharvest diseases in kiwifruit.

由灰霉病菌（Botrytis cinerea）感染引起的灰霉病严重影响采后猕猴桃，导致变质和食品安全问题。同时，茉莉酸甲酯（MeJA）可诱导植物产生防御反应。本研究旨在确定诱导猕猴桃抗病的最佳 MeJA 浓度。值得注意的是，在经 MeJA 处理的猕猴桃中检测到了多种植物防御酶。此外，研究还发现 0.01 mol/L MeJA 能通过提高抗氧化酶的活性来增强猕猴桃的抗病性。广泛的靶向代谢组学分析显示，不同处理组之间存在 62-64 个差异表达代谢物（DEMs）。此外，RNA-seq 分析还发现这些组间存在 930-2900 个差异表达基因（DEG）。随后，结合 DEG 和 DEM 分析，发现苯丙酮生物合成和植物激素信号转导是与 MeJA 诱导的抗性相关的关键传导途径。总之，这些发现确定了 MeJA 诱导猕猴桃抗性的机制，为安全有效地控制猕猴桃采后病害提供了理论依据。

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引用次数: 0

Fine localization and functional differentiation of exocarp cell clusters during fruit senescence revealed by single-cell and spatial transcriptomics 单细胞和空间转录组学揭示果实衰老过程中外果皮细胞簇的精细定位和功能分化

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2024-09-23 DOI: 10.1016/j.postharvbio.2024.113223

Jingyu Jia , Enyan Chen , Yajing Tian , Jiahao Shen , Xinyue Pang , Xin Li

The differentiation trajectories of 13 cell clusters in H. undatus during senescence have been revealed by single-cell transcriptome (scRNA-seq) analysis, and the five cell clusters in the exocarp have been found to induce resistance mainly. However, the precise localization and functional differentiation of these five cell clusters remain unclear. In this study, phenotypic changes in the exocarp were recorded, and the ROS production and flavonoid biosynthesis in the exocarp were elucidated. The trajectories of the cell clusters involved in exocarp function were further revealed in scRNA-seq profiles. Through integrated analysis of scRNA-seq and spatial RNA-seq data at resolutions of 0.5, 0.6, and 1.5, using algorithms SingleR, SciBet, CARD, and RCTD, the fine localization of these clusters from the outer to inner layers was achieved. RNA-FISH was used to validate the specific expression of marker genes HuSP2 and HuCEL2 in different locations of the exocarp. Finally, the differentiation trajectories of the five cell clusters were analyzed by selecting highly correlated cells with specific functions for each cluster. The core roles of HuPRP and HuCRRSP55 in the gene regulatory networks of exocarp clusters were revealed. A hypothesis that the five clusters in the exocarp participate in the induction of resistance was proposed.

单细胞转录组（scRNA-seq）分析揭示了H. undatus在衰老过程中13个细胞簇的分化轨迹，发现外果皮中的5个细胞簇主要诱导抗性。然而，这五个细胞簇的精确定位和功能分化仍不清楚。本研究记录了外果皮的表型变化，并阐明了外果皮中 ROS 的产生和黄酮类化合物的生物合成。scRNA-seq图谱进一步揭示了参与外果皮功能的细胞群的轨迹。通过使用SingleR、SciBet、CARD和RCTD算法对分辨率为0.5、0.6和1.5的scRNA-seq和空间RNA-seq数据进行综合分析，实现了这些细胞簇从外层到内层的精细定位。利用 RNA-FISH 验证了标记基因 HuSP2 和 HuCEL2 在外果皮不同位置的特异性表达。最后，通过为每个细胞簇选择具有特定功能的高度相关细胞，分析了五个细胞簇的分化轨迹。揭示了HuPRP和HuCRRSP55在外果皮细胞簇基因调控网络中的核心作用。提出了外果皮中的五个细胞簇参与抗性诱导的假说。

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引用次数: 0

CpAGL18 is involved in the cross-talk of ethylene and auxin signaling in the regulation of fruit ripening in papaya CpAGL18 参与乙烯和辅助素信号在木瓜果实成熟调控中的交叉作用

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2024-09-23 DOI: 10.1016/j.postharvbio.2024.113221

Jiahui Cai , Ziling Wu , Wang Zheng , Qiunan Zhu , Zunyang Song , Weixin Chen , Xueping Li , Xiaoyang Zhu

引用次数: 0

Influence of pH and temperature in a heated flooder application on imazalil residue loading, citrus green mould infection and sporulation inhibition 加热淹没器中的 pH 值和温度对咪鲜胺残留量、柑橘绿色霉菌感染和孢子抑制的影响

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2024-09-23 DOI: 10.1016/j.postharvbio.2024.113227

Catherine Savage , Arno Erasmus , Wilma du Plooy , Cheryl Lennox , Paul H. Fourie

Green mould (caused by Penicillium digitatum) is the largest contributor to loss due to postharvest decay of citrus fruit. Imazalil (IMZ) in the IMZ sulphate formulation is the most important fungicide in green mould management in citrus packhouses. Studies have highlighted the importance of pH and temperature of IMZ sulphate solutions, as well as the exposure time of fruit to the solution, but were focussed on dip application as this was the most common IMZ application method. The heated flooder (in-line drench or cascade) has widely replaced the use of a dip or bath for IMZ application in citrus packhouses globally; however, studies on its optimal use and efficacy were needed. Variables that were studied included the effects of pH (3, 4, 5 and 6), the temperature of the solution (45, 55 and 65 °C at pH 6), and concentration (250 or 500 mg L⁻¹) in a time of 8 s. Imazalil residue loading models were developed for the effects of pH and temperature. Imazalil residue levels loaded on Satsuma mandarin, lemon, navel and Valencia orange fruit increased as pH, temperature range and concentration were increased, and were generally in the range between 0.4 and 3.0 mg kg⁻¹. The South African Maximum Residue Limit (MRL) of 5.0 mg kg⁻¹ was often exceeded at a pH of 6 at temperatures of 55 and 65 °C. At residue levels below the MRL, IMZ application using the heated flooder offered excellent curative action on 24-hour-old infections, excellent protective control, as well as sporulation inhibition. Sporulation inhibition of P. digitatum from green mould lesions was modelled on residue levels, and 90 % inhibition was obtained at residue levels of 1.84–2.47 mg kg⁻¹.

绿色霉菌（由数字青霉引起）是柑橘类水果采后腐烂造成损失的最大原因。IMZ硫酸盐制剂中的咪鲜胺（IMZ）是柑橘包装车间管理绿霉菌最重要的杀菌剂。研究强调了硫酸异丙嗪溶液的 pH 值和温度以及果实与溶液接触时间的重要性，但重点放在浸泡施用上，因为这是最常见的异丙嗪施用方法。在全球范围内，柑橘包装车间使用加热灌注器（在线淋洗或串联）施用 IMZ 时，已广泛取代了浸泡或水浴法；不过，还需要对其最佳使用方法和功效进行研究。研究的变量包括 pH 值（3、4、5 和 6）、溶液温度（pH 值为 6 时为 45、55 和 65 °C）和浓度（250 或 500 毫克/升）在 8 秒内的影响。针对 pH 值和温度的影响建立了咪唑啉残留物负载模型。随着 pH 值、温度范围和浓度的增加，沙糖桔、柠檬、脐橙和瓦伦西亚橙果实上的咪草烟残留量也随之增加，一般在 0.4 至 3.0 毫克/千克之间。在 pH 值为 6、温度为 55 和 65 °C 的条件下，往往会超过南非最高残留限量 (MRL) 5.0 毫克/千克。在残留量低于最高残留限量的情况下，使用加热淹没器施用 IMZ 对 24 小时感染的病菌有很好的治疗作用、很好的保护性控制作用以及孢子抑制作用。根据残留水平模拟了绿色霉菌病变中的地衣芽孢抑制作用，当残留水平为 1.84-2.47 毫克/千克时，抑制率为 90%。

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引用次数: 0

Exogenous deferoxamine mesylate suppresses iron-dependent ferroptosis in postharvest fruit rot of blueberry caused by Alternaria alternata and Fusarium fujikuroi 外源性甲磺酸去铁胺可抑制由交替孢属和藤黄镰刀菌引起的蓝莓采后果实腐烂病中的铁依赖性铁变态反应

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2024-09-21 DOI: 10.1016/j.postharvbio.2024.113216

Shuang Gu , Lin Xie , Xuerong Sheng , Guiqing Xing , Xiangyang Wang

Plant development relies heavily on regulated cell death, which is crucial for specific plant responses to biological stresses. Ferroptosis, an iron-dependent, oxidative, and non-apoptotic cell death form, has been recently discovered in animal cells. In this study, we investigated whether a ferroptosis-like process could be relevant to cell death in postharvest fruit rot of blueberry caused by Alternaria alternata and Fusarium fujikuroi. The results showed that deferoxamine mesylate and glutathione (GSH) treated fruit showed enhanced disease resistance with lower lesion areas, whereas an opposite result was observed in hydrogen peroxide (H₂O₂) treatment, indicating that intracellular ferroptosis might exist in the fungal infected blueberry fruit. Compared to control, deferoxamine mesylate or GSH treatment improved chlorophyll ﬂuorescence parameters, which contributed to the stability of photosynthesis. In terms of ferroptosis pathway in the adjacent area of infected fruit, deferoxamine mesylate suppressed an iron-dependent cell death pathway that was characterized by depletion of H₂O₂ and MDA and accumulation of GSH, as well as the reduction of ferrous ions and total iron ions. Meanwhile, deferoxamine mesylate could effectively delay the decrease rate of unsaturation value and key phospholipids of fungi infected fruit during storage, which possibly maintained the integrity of cell membrane. Due to the serious rot of the lesion area, the peroxidation of membrane lipids was severe, accompanied by metabolic disorders. These combined results demonstrated that deferoxamine mesylate treatment could reduce Fenton reaction by chelating Fe²⁺ and avoid excessive accumulation of reactive oxygen species (ROS), suppress iron-dependent ferroptosis, thereby maintaining fruit quality and reducing postharvest diseases.

植物的生长发育在很大程度上依赖于受调控的细胞死亡，这对于植物对生物压力的特定反应至关重要。最近在动物细胞中发现了一种铁依赖性、氧化性和非凋亡性细胞死亡形式--铁凋亡。在这项研究中，我们调查了类似铁凋亡的过程是否与由交替孢属（Alternaria alternata）和富坚黑镰刀菌（Fusarium fujikuroi）引起的蓝莓采后果实腐烂病中的细胞死亡有关。结果表明，甲磺酸去铁胺和谷胱甘肽（GSH）处理的果实表现出更强的抗病性，病变面积更小，而过氧化氢（H2O2）处理的果实则表现出相反的结果，这表明在真菌感染的蓝莓果实中可能存在细胞内的铁突变。与对照组相比，甲磺酸去铁胺或 GSH 处理改善了叶绿素荧光参数，有助于光合作用的稳定。在感染果实邻近区域的铁突变途径方面，甲磺酸去铁胺抑制了铁依赖性细胞死亡途径，该途径的特征是 H2O2 和 MDA 的耗竭和 GSH 的积累，以及亚铁离子和总铁离子的减少。同时，甲磺酸去铁胺能有效延缓真菌感染果实在贮藏过程中不饱和值和关键磷脂的下降速度，从而可能保持细胞膜的完整性。由于病变区域腐烂严重，膜脂过氧化反应严重，并伴有代谢紊乱。这些综合结果表明，甲磺酸去铁胺处理可通过螯合 Fe2+ 减少芬顿反应，避免活性氧（ROS）的过度积累，抑制铁依赖性铁变态反应，从而保持果实品质，减少采后病害。

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引用次数: 0

Methyl jasmonate activated regulatory module Ma14-3-3e-MbHLH130-MbACO13/MbACS7 promoting ethylene biosynthesis and fruit ripening in banana 茉莉酸甲酯激活调控模块 Ma14-3-3e-MbHLH130-MbACO13/MbACS7 促进香蕉乙烯的生物合成和果实成熟

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2024-09-21 DOI: 10.1016/j.postharvbio.2024.113215

Meiying Li , Yan Yan , Liwang Zeng , Zhengnan Xie , Zehong Ding , Jinghao Yang , Yu Wang , Jianxiang Ma , Kaisen Huo , Xiaoliang Yang , Qiyu Xia , Xiao xue Ye , Chaochao Li , Zhiqiang Jin , Licheng Ren , Wei Hu

Methyl jasmonate (MeJA), an important phytohormone, plays a vital role in many biological processes. However, its effect and mechanism in regulating ethylene synthesis and fruit ripening in banana remain unknown. In this research, we found that exogenous MeJA accelerated postharvest ripening of banana, which coincided with increased ethylene production and upregulation of MbACS7 and MbACO13 expression. MabHLH130 directly interacted with and stimulated the transcription of MbACS7 and MbACO13. Additionally, MabHLH130 interacted with Ma14-3-3e through the RHSSSP motif. Overexpression of either MabHLH130 or Ma14-3–3e in tomato promoted ethylene production and fruit ripening, and this effect was enhanced by exogenous MeJA treatment. Furthermore, the Ma14-3–3e-MabHLH130 interaction module activated MbACS7 and MbACO13 transcription, which was enhanced by exogenous MeJA treatment. Taken together, these results demonstrated that the MeJA-responsive Ma14-3–3e-MabHLH130-MbACS7/MbACO13 module promoted ethylene biosynthesis and fruit ripening in bananas, providing valuable genetic targets for breeding programs aimed at extending fruit shelf-life.

茉莉酸甲酯（MeJA）是一种重要的植物激素，在许多生物过程中发挥着重要作用。然而，它在调节乙烯合成和香蕉果实成熟方面的作用和机制仍不清楚。本研究发现，外源 MeJA 可加速香蕉采后成熟，这与乙烯产量增加以及 MbACS7 和 MbACO13 表达上调有关。MabHLH130直接与MbACS7和MbACO13相互作用并刺激其转录。此外，MabHLH130通过RHSSSP基序与Ma14-3-3e相互作用。在番茄中过表达 MabHLH130 或 Ma14-3-3e 可促进乙烯的产生和果实成熟，外源 MeJA 处理可增强这种效应。此外，Ma14-3-3e-MabHLH130 相互作用模块激活了 MbACS7 和 MbACO13 的转录，外源 MeJA 处理增强了这种作用。综上所述，这些结果表明，MeJA响应的Ma14-3-3e-MabHLH130-MbACS7/MbACO13模块促进了香蕉的乙烯生物合成和果实成熟，为旨在延长果实货架期的育种计划提供了有价值的遗传目标。

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引用次数: 0