Postharvest Biology and Technology最新文献

英文中文

Melatonin treatment delays postharvest senescence of ‘Dayagan’ hybrid citrus fruit by enhancing reactive oxygen species-scavenging capacity and preserving higher unsaturated fatty acid content

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2025-01-14 DOI: 10.1016/j.postharvbio.2025.113400

Min Hong , Lian Zhou , Hui Zhang , Linhua Huang , Mingyang He

This study sought to investigate whether melatonin (MT)-induced alterations in membrane fatty acid and reactive oxygen species (ROS) metabolism are associated with senescence of citrus fruits during storage. The findings demonstrated that applying 1 g L⁻¹ MT reduced the respiration rate, delayed weight loss and decay, and maintained better inner fruit quality. MT alleviated oxidative damage and protected membrane stability by lowering malondialdehyde and electrolyte leakage levels. MT increased the antioxidant content (ascorbic acid, total phenols, and total flavonoids) along with enhancing the ferric ion-reducing antioxidant power and 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity and promoted the activity of enzymes like superoxide dismutase, peroxidase, and catalase, thereby decreasing ROS accumulation. MT-treated fruits had higher unsaturated fatty acid (USFA) content by regulating lipase and lipoxygenase, which contributed to improving the ratio of USFAs to saturated fatty acids and the double-bond index. Furthermore, a significant relationship between membrane stability and postharvest fruit senescence was confirmed. These results suggest that MT inhibits postharvest physiological senescence and enhances the storage performance of citrus fruits by enhancing ROS-scavenging capacity and preserving higher unsaturated fatty acid content to alleviate membrane oxidative damage and maintain membrane stability.

{"title":"Melatonin treatment delays postharvest senescence of ‘Dayagan’ hybrid citrus fruit by enhancing reactive oxygen species-scavenging capacity and preserving higher unsaturated fatty acid content","authors":"Min Hong , Lian Zhou , Hui Zhang , Linhua Huang , Mingyang He","doi":"10.1016/j.postharvbio.2025.113400","DOIUrl":"10.1016/j.postharvbio.2025.113400","url":null,"abstract":"<div><div>This study sought to investigate whether melatonin (MT)-induced alterations in membrane fatty acid and reactive oxygen species (ROS) metabolism are associated with senescence of citrus fruits during storage. The findings demonstrated that applying 1 g L<sup>−1</sup> MT reduced the respiration rate, delayed weight loss and decay, and maintained better inner fruit quality. MT alleviated oxidative damage and protected membrane stability by lowering malondialdehyde and electrolyte leakage levels. MT increased the antioxidant content (ascorbic acid, total phenols, and total flavonoids) along with enhancing the ferric ion-reducing antioxidant power and 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity and promoted the activity of enzymes like superoxide dismutase, peroxidase, and catalase, thereby decreasing ROS accumulation. MT-treated fruits had higher unsaturated fatty acid (USFA) content by regulating lipase and lipoxygenase, which contributed to improving the ratio of USFAs to saturated fatty acids and the double-bond index. Furthermore, a significant relationship between membrane stability and postharvest fruit senescence was confirmed. These results suggest that MT inhibits postharvest physiological senescence and enhances the storage performance of citrus fruits by enhancing ROS-scavenging capacity and preserving higher unsaturated fatty acid content to alleviate membrane oxidative damage and maintain membrane stability.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113400"},"PeriodicalIF":6.4,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143101879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated transcriptomic and metabolomic analysis of delayed leaf yellowing in postharvest pak choi (Brassica rapa subsp. chinensis) by 2-ethylhexanol (2-EH)

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2025-01-14 DOI: 10.1016/j.postharvbio.2025.113403

Mina Wang , Xiaozhen Yue , Lingda Yu , Zixin Lin , Shuzhi Yuan , Xiaodi Xu , Jinhua Zuo , Bihong Feng , Qing Wang

The antioxidant properties of 2-ethylhexanol (2-EH) have the potential to maintain the quality of pak choi during storage. Therefore, the effect of 2-EH on pak choi during storage for 7 d at 20 °C and 90 % relative humidity (RH) was investigated. Results demonstrated that fumigation of pak choi with 2-EH maintained the quality of pak choi by reducing the respiration rate, ethylene generation, the level of malondialdehyde (MDA), and electrolyte leakage, while increasing the level of total soluble solids (TSS), ascorbic acid, flavonoids, total phenolics, and alpha-linolenic acid, relative to the untreated control. Transcriptomic and metabolomic analyses revealed that 2-EH mitigated chlorophyll degradation by up-regulating the expression of CRD1, POR, DVR, CHLP, and CHLG in the porphyrin and chlorophyll metabolism pathways, and by down-regulating SGR2. 2-EH also increased antioxidant enzyme activity and up-regulated the expression of PAL, C4H, BRT1, CYP98A, CCR, FLS, and LDOX in the phenylpropanoid and flavonoid biosynthesis pathways, increased phenolic content, and enhanced antioxidant capacity, thereby, mitigating excessive accumulation of reactive oxygen species (ROS). The 2-EH treatment also up-regulated HPL1 and AOS genes in the alpha-linolenic acid metabolic pathway and down-regulated the expression of ACOX and MFP2, thus, increasing alpha-linolenic acid content and maintaining cell membrane structure. In summary, our study demonstrated that 2-EH maintained pak choi quality during storage and provided insights into the underlying molecular and metabolic changes associated with delayed yellowing of pak choi by 2-EH.

{"title":"Integrated transcriptomic and metabolomic analysis of delayed leaf yellowing in postharvest pak choi (Brassica rapa subsp. chinensis) by 2-ethylhexanol (2-EH)","authors":"Mina Wang , Xiaozhen Yue , Lingda Yu , Zixin Lin , Shuzhi Yuan , Xiaodi Xu , Jinhua Zuo , Bihong Feng , Qing Wang","doi":"10.1016/j.postharvbio.2025.113403","DOIUrl":"10.1016/j.postharvbio.2025.113403","url":null,"abstract":"<div><div>The antioxidant properties of 2-ethylhexanol (2-EH) have the potential to maintain the quality of pak choi during storage. Therefore, the effect of 2-EH on pak choi during storage for 7 d at 20 °C and 90 % relative humidity (RH) was investigated. Results demonstrated that fumigation of pak choi with 2-EH maintained the quality of pak choi by reducing the respiration rate, ethylene generation, the level of malondialdehyde (MDA), and electrolyte leakage, while increasing the level of total soluble solids (TSS), ascorbic acid, flavonoids, total phenolics, and alpha-linolenic acid, relative to the untreated control. Transcriptomic and metabolomic analyses revealed that 2-EH mitigated chlorophyll degradation by up-regulating the expression of <em>CRD1</em>, <em>POR</em>, <em>DVR</em>, <em>CHLP</em>, and <em>CHLG</em> in the porphyrin and chlorophyll metabolism pathways, and by down-regulating <em>SGR2</em>. 2-EH also increased antioxidant enzyme activity and up-regulated the expression of <em>PAL</em>, <em>C4H</em>, <em>BRT1</em>, <em>CYP98A</em>, <em>CCR</em>, <em>FLS</em>, and <em>LDOX</em> in the phenylpropanoid and flavonoid biosynthesis pathways, increased phenolic content, and enhanced antioxidant capacity, thereby, mitigating excessive accumulation of reactive oxygen species (ROS). The 2-EH treatment also up-regulated <em>HPL1</em> and <em>AOS</em> genes in the alpha-linolenic acid metabolic pathway and down-regulated the expression of <em>ACOX</em> and <em>MFP2,</em> thus, increasing alpha-linolenic acid content and maintaining cell membrane structure. In summary, our study demonstrated that 2-EH maintained pak choi quality during storage and provided insights into the underlying molecular and metabolic changes associated with delayed yellowing of pak choi by 2-EH.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113403"},"PeriodicalIF":6.4,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143101881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antifungal and mechanism of rose essential oil against Monilinia fructicola caused brown rot of peach fruit

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2025-01-14 DOI: 10.1016/j.postharvbio.2025.113398

Dedong Min , Huanyu Wu , Meng Xu , Peng Leng , Jie Sun , Yun-Guo Liu

Peach fruits exhibit high susceptibility to fungal pathogens, significantly limiting their storage period and resulting in substantial economic losses. Identification of fungal strains affecting peaches fruit decay revealed that Monilinia fructicola is the most aggressive pathogen. Rose essential oil (REO) has demonstrated antimicrobial activity against a wide range of microorganisms; however, its antifungal properties and mechanisms against M. fructicola have not been thoroughly investigated. In this study, both in vivo and in vitro experiments were performed, revealing that REO possesses strong antifungal activity against M. fructicola, effectively inhibiting brown rot in postharvest peaches. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analyses indicated that REO treatment caused significant damage to the morphology and ultrastructure of M. fructicola. REO compromised cell membrane integrity, leading to increased electrical conductivity and nucleic acid concentrations. REO treatment also resulted in decreased activities of ATPase, malate dehydrogenase (MDH), and succinate dehydrogenase (SDH), adversely affecting the respiratory processes of M. fructicola. Furthermore, REO treatment also inhibited the decrease of firmness and total phenolic content. Therefore, REO presents a promising and environmentally friendly alternative to conventional fungicides for the control of brown rot in peach fruits.

{"title":"Antifungal and mechanism of rose essential oil against Monilinia fructicola caused brown rot of peach fruit","authors":"Dedong Min , Huanyu Wu , Meng Xu , Peng Leng , Jie Sun , Yun-Guo Liu","doi":"10.1016/j.postharvbio.2025.113398","DOIUrl":"10.1016/j.postharvbio.2025.113398","url":null,"abstract":"<div><div>Peach fruits exhibit high susceptibility to fungal pathogens, significantly limiting their storage period and resulting in substantial economic losses. Identification of fungal strains affecting peaches fruit decay revealed that <em>Monilinia fructicola</em> is the most aggressive pathogen. Rose essential oil (REO) has demonstrated antimicrobial activity against a wide range of microorganisms; however, its antifungal properties and mechanisms against <em>M. fructicola</em> have not been thoroughly investigated. In this study, both <em>in vivo</em> and <em>in vitro</em> experiments were performed, revealing that REO possesses strong antifungal activity against <em>M. fructicola</em>, effectively inhibiting brown rot in postharvest peaches. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analyses indicated that REO treatment caused significant damage to the morphology and ultrastructure of <em>M. fructicola</em>. REO compromised cell membrane integrity, leading to increased electrical conductivity and nucleic acid concentrations. REO treatment also resulted in decreased activities of ATPase, malate dehydrogenase (MDH), and succinate dehydrogenase (SDH), adversely affecting the respiratory processes of <em>M. fructicola</em>. Furthermore, REO treatment also inhibited the decrease of firmness and total phenolic content. Therefore, REO presents a promising and environmentally friendly alternative to conventional fungicides for the control of brown rot in peach fruits.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113398"},"PeriodicalIF":6.4,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143101878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AI-driven deep learning framework for shelf life prediction of edible mushrooms

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2025-01-12 DOI: 10.1016/j.postharvbio.2025.113396

Shima Javanmardi , Seyed-Hassan Miraei Ashtiani

Fresh edible mushrooms are highly perishable, prone to microbial contamination, and have a limited shelf life, making it essential to assess their freshness to ensure food safety and minimize waste. Additionally, the freshness of mushrooms has a significant impact on consumer health and market prices. This study introduces and evaluates a computer vision-based application utilizing convolutional neural networks (CNNs) to estimate the shelf life of three widely consumed mushroom varieties: white button, shiitake, and oyster, stored at 4 ± 1 °C. To improve classification accuracy and reduce training costs, transfer learning was employed to fine-tune CNN models, including EfficientNet, NASNetLarge, ResNet-50, Inception-V3, and MobileNet-V2. ResNet-50 demonstrated the highest performance for white button and oyster mushrooms, with overall accuracies of 94.10 % and 89.11 %, respectively, processing 1960 images in 6.39 min for the former and 8.70 min for the latter. For shiitake mushrooms, MobileNet-V2 showed superior performance with an accuracy of 86.36 % and a processing time of 5.73 min. Integrating digital imaging with CNN methods offers a reliable and efficient approach for rapid and precise evaluation of mushroom freshness. This technology can significantly improve monitoring during the postharvest storage and distribution stages, facilitating informed decision-making and timely actions to reduce spoilage.

{"title":"AI-driven deep learning framework for shelf life prediction of edible mushrooms","authors":"Shima Javanmardi , Seyed-Hassan Miraei Ashtiani","doi":"10.1016/j.postharvbio.2025.113396","DOIUrl":"10.1016/j.postharvbio.2025.113396","url":null,"abstract":"<div><div>Fresh edible mushrooms are highly perishable, prone to microbial contamination, and have a limited shelf life, making it essential to assess their freshness to ensure food safety and minimize waste. Additionally, the freshness of mushrooms has a significant impact on consumer health and market prices. This study introduces and evaluates a computer vision-based application utilizing convolutional neural networks (CNNs) to estimate the shelf life of three widely consumed mushroom varieties: white button, shiitake, and oyster, stored at 4 ± 1 °C. To improve classification accuracy and reduce training costs, transfer learning was employed to fine-tune CNN models, including EfficientNet, NASNetLarge, ResNet-50, Inception-V3, and MobileNet-V2. ResNet-50 demonstrated the highest performance for white button and oyster mushrooms, with overall accuracies of 94.10 % and 89.11 %, respectively, processing 1960 images in 6.39 min for the former and 8.70 min for the latter. For shiitake mushrooms, MobileNet-V2 showed superior performance with an accuracy of 86.36 % and a processing time of 5.73 min. Integrating digital imaging with CNN methods offers a reliable and efficient approach for rapid and precise evaluation of mushroom freshness. This technology can significantly improve monitoring during the postharvest storage and distribution stages, facilitating informed decision-making and timely actions to reduce spoilage.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113396"},"PeriodicalIF":6.4,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143101859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High relative humidity storage mitigates chilling injury in zucchini fruit via regulating the CpMYB63-mediated cell wall degradation

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2025-01-11 DOI: 10.1016/j.postharvbio.2025.113388

Xiaoxia Zuo , Jing Wang , Zhengguo Wu, Peng Jin, Yonghua Zheng

The effect of high relative humidity (HRH) storage on cell wall degradation and the possible regulation mechanism mediated by CpMYB63 in zucchini fruit during cold storage (4 ℃) were investigated. The present results showed that HRH (RH: 96–100 %) storage decreased weight loss and firmness loss together with the chilling injury (CI) of zucchini under chilling stress. HRH storage inhibited the solubilization of cell wall polysaccharides induced by the CI development, as shown by the lower level of water-soluble faction and the higher levels of Na₂CO₃-soluble faction and hemicellulose in HRH-fruit than in the control-fruit (RH: 72–76 %). Meanwhile, the increase in activities of pectin methylesterase and polygalacturonase in chilled zucchini fruit is also depressed by HRH. Furthermore, HRH maintained lower expression levels of CpPME3, CpPG1 as well as CpMYB63 in zucchini during cold storage. The CpMYB63 was characterized as a nucleus-localized protein and positively regulated the transcription of CpPME3 and CpPG1. Taken together, the results suggested that the inhibitory effect of HRH on cell wall degradation contributed to the decreased CI in zucchini under chilling stress, which was associated with the lower level of CpMYB63 expression and the weakened transcription activation of CpMYB63 to CpPME3 and CpPG1 caused by HRH storage.

{"title":"High relative humidity storage mitigates chilling injury in zucchini fruit via regulating the CpMYB63-mediated cell wall degradation","authors":"Xiaoxia Zuo , Jing Wang , Zhengguo Wu, Peng Jin, Yonghua Zheng","doi":"10.1016/j.postharvbio.2025.113388","DOIUrl":"10.1016/j.postharvbio.2025.113388","url":null,"abstract":"<div><div>The effect of high relative humidity (HRH) storage on cell wall degradation and the possible regulation mechanism mediated by CpMYB63 in zucchini fruit during cold storage (4 ℃) were investigated. The present results showed that HRH (RH: 96–100 %) storage decreased weight loss and firmness loss together with the chilling injury (CI) of zucchini under chilling stress. HRH storage inhibited the solubilization of cell wall polysaccharides induced by the CI development, as shown by the lower level of water-soluble faction and the higher levels of Na<sub>2</sub>CO<sub>3</sub>-soluble faction and hemicellulose in HRH-fruit than in the control-fruit (RH: 72–76 %). Meanwhile, the increase in activities of pectin methylesterase and polygalacturonase in chilled zucchini fruit is also depressed by HRH. Furthermore, HRH maintained lower expression levels of <em>CpPME3</em>, <em>CpPG1</em> as well as <em>CpMYB63</em> in zucchini during cold storage. The CpMYB63 was characterized as a nucleus-localized protein and positively regulated the transcription of <em>CpPME3</em> and <em>CpPG1</em>. Taken together, the results suggested that the inhibitory effect of HRH on cell wall degradation contributed to the decreased CI in zucchini under chilling stress, which was associated with the lower level of <em>CpMYB63</em> expression and the weakened transcription activation of CpMYB63 to <em>CpPME3</em> and <em>CpPG1</em> caused by HRH storage.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113388"},"PeriodicalIF":6.4,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143101862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prediction of core browning and fruit firmness in stored ‘Braeburn’ apples: Multivariate time series classification

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2025-01-11 DOI: 10.1016/j.postharvbio.2024.113381

Lukas Munser , Roy McCormick , Grigory Devadze , Nils Siefen , Stefan Streif , Konni Biegert

Physiological storage disorders affect many commercial apple cultivars and cause economic loss and increase food waste. In general, robust prediction methods for disorders such as ‘Braeburn’ internal browning, specifically core browning as it is most prevalent in Southwest Germany, do not exist. A good understanding of the causal factors is still lacking, but symptom development varies markedly between orchards. This work describes a combined modeling and machine learning approach to link pre-harvest environmental, tree and management factors with post-harvest storage outcomes to predict internal browning and fruit firmness. Non-destructive spectral time series data were collected from ‘Braeburn’ apples over seven seasons from 2016 to 2023 from orchards located in Southwest Germany. These data were used as reference measurements to construct a weather-based model to produce multivariate time series for changes in four fruit quality parameters: chlorophyll, anthocyanins, soluble solids and dry matter content during pre-harvest growth and development on the tree. The multivariate times-series were then used as input into a dynamic time warping (DTW)-based k-nearest neighbor (kNN) classifier. The data set contains 1729 objects and the classifier shows reliable good cross validated prediction results for internal browning and firmness, respectively. After DTW and dimension reduction mapping, 2D cluster analysis shows each season as being clearly separated in multidimensional space. However, single season learning cannot be generalized to other seasons. The multidimensional distance between browning clusters is related to the average rate of change in the spectral scanning data during the 50 d pre-harvest period. Fruit lots with high browning show a higher mean cumulative change.

{"title":"Prediction of core browning and fruit firmness in stored ‘Braeburn’ apples: Multivariate time series classification","authors":"Lukas Munser , Roy McCormick , Grigory Devadze , Nils Siefen , Stefan Streif , Konni Biegert","doi":"10.1016/j.postharvbio.2024.113381","DOIUrl":"10.1016/j.postharvbio.2024.113381","url":null,"abstract":"<div><div>Physiological storage disorders affect many commercial apple cultivars and cause economic loss and increase food waste. In general, robust prediction methods for disorders such as ‘Braeburn’ internal browning, specifically core browning as it is most prevalent in Southwest Germany, do not exist. A good understanding of the causal factors is still lacking, but symptom development varies markedly between orchards. This work describes a combined modeling and machine learning approach to link pre-harvest environmental, tree and management factors with post-harvest storage outcomes to predict internal browning and fruit firmness. Non-destructive spectral time series data were collected from ‘Braeburn’ apples over seven seasons from 2016 to 2023 from orchards located in Southwest Germany. These data were used as reference measurements to construct a weather-based model to produce multivariate time series for changes in four fruit quality parameters: chlorophyll, anthocyanins, soluble solids and dry matter content during pre-harvest growth and development on the tree. The multivariate times-series were then used as input into a dynamic time warping (DTW)-based k-nearest neighbor (kNN) classifier. The data set contains 1729 objects and the classifier shows reliable good cross validated prediction results for internal browning and firmness, respectively. After DTW and dimension reduction mapping, 2D cluster analysis shows each season as being clearly separated in multidimensional space. However, single season learning cannot be generalized to other seasons. The multidimensional distance between browning clusters is related to the average rate of change in the spectral scanning data during the 50 d pre-harvest period. Fruit lots with high browning show a higher mean cumulative change.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113381"},"PeriodicalIF":6.4,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143101860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to ''Tyr-Asp enhances chilling tolerance in banana fruit by modulating redox homeostasis'' [Postharvest Biol. Technol., vol., 222, April 2025, 113338]

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2025-01-11 DOI: 10.1016/j.postharvbio.2025.113395

Yanxuan Zhu , Yiejie Zhou , Zhiwei Li , Ruimin Zhong , Yueming Jiang , Guoxiang Jiang , Xinguo Su

引用次数: 0

Transcriptomics and physiological indicators analyzed the delayed effect of carboxymethyl chitosan on lignification of okra during postharvest cold storage

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2025-01-11 DOI: 10.1016/j.postharvbio.2025.113394

Lijuan Wei, Shunjiao Gao, Fan Li, Xinyue Zhang, Yue Peng, Yaowen Liu, Yiqin Zhang, Wen Qin

Lignification is one of the key factors leading to postharvest quality deterioration of fruit and vegetables. In this study, the effects of carboxymethyl chitosan (CMCS) on nutritional characteristics, cell wall substances, key enzyme activities, transcriptome and related gene expression levels of okra during postharvest low temperature storage were investigated. The results showed that CMCS could maintain the nutritional quality of postharvest okra, delay the accumulation of rigid components (lignin, cellulose, hemicellulose and pectin) in cell wall, and inhibit the enzyme activities of phenylpropanoid pathway (PAL, 4CL, CAD and POD). Transcriptome data analysis and qRT-PCR results showed that CMCS down-regulated the expression levels of lignin synthesis-related genes (AePAL, AeC4H, Ae4CL, AeCAD, AeCOMT, AePOD). MYB is an important transcription factor (TF) regulating lignin accumulation. The results of gene promoter analysis in qRT-PCR showed that 14 genes contained multiple MYB cis-acting elements. Compared with CK, CMCS inhibited the expression of multiple upstream AeMYBs. Collectively, CMCS is a promising green preservative by inhibiting the metabolism of nutrients, the enzyme activities of the phenylpropanoid pathway, and the expression levels of related genes and TFs, thereby delaying the accumulation of lignin during postharvest cold storage of okra and maintaining postharvest quality.

{"title":"Transcriptomics and physiological indicators analyzed the delayed effect of carboxymethyl chitosan on lignification of okra during postharvest cold storage","authors":"Lijuan Wei, Shunjiao Gao, Fan Li, Xinyue Zhang, Yue Peng, Yaowen Liu, Yiqin Zhang, Wen Qin","doi":"10.1016/j.postharvbio.2025.113394","DOIUrl":"10.1016/j.postharvbio.2025.113394","url":null,"abstract":"<div><div>Lignification is one of the key factors leading to postharvest quality deterioration of fruit and vegetables. In this study, the effects of carboxymethyl chitosan (CMCS) on nutritional characteristics, cell wall substances, key enzyme activities, transcriptome and related gene expression levels of okra during postharvest low temperature storage were investigated. The results showed that CMCS could maintain the nutritional quality of postharvest okra, delay the accumulation of rigid components (lignin, cellulose, hemicellulose and pectin) in cell wall, and inhibit the enzyme activities of phenylpropanoid pathway (PAL, 4CL, CAD and POD). Transcriptome data analysis and qRT-PCR results showed that CMCS down-regulated the expression levels of lignin synthesis-related genes (<em>AePAL, AeC4H, Ae4CL, AeCAD, AeCOMT, AePOD</em>). MYB is an important transcription factor (TF) regulating lignin accumulation. The results of gene promoter analysis in qRT-PCR showed that 14 genes contained multiple MYB cis-acting elements. Compared with CK, CMCS inhibited the expression of multiple upstream <em>AeMYBs</em>. Collectively, CMCS is a promising green preservative by inhibiting the metabolism of nutrients, the enzyme activities of the phenylpropanoid pathway, and the expression levels of related genes and TFs, thereby delaying the accumulation of lignin during postharvest cold storage of okra and maintaining postharvest quality.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113394"},"PeriodicalIF":6.4,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143101861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exogenous NaHS maintains storage quality of Prunus salicina ‘Wushan plum’ by regulating the antioxidant system, endogenous H2S, ethylene and NO metabolism

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2025-01-10 DOI: 10.1016/j.postharvbio.2025.113391

Saiying Han , Ling Liu , Lilei Wang , Jin Han , Yeru Ai , Huali Wang , Kaifang Zeng , Jian Ming , Lili Deng

Prunus salicina ‘Wushan plum’ is a climacteric fruit, which rapidly ripens under the action of ethylene after harvesting, resulting in quality deterioration that seriously restricts the sustainable development of the industry. In order to examine the potential role of sodium hydrosulfide (NaHS, the donor of exogenous H₂S) in slowing down the ripening and senescence process of plum fruit, the changes in storage quality, antioxidant capacity, endogenous signals and gene expression levels were evaluated during storage at 25 °C for twelve days. The results indicated that NaHS treatment delayed the coloration of plum fruit and maintained higher firmness, titratable acid content (TA) and marketable fruit rate. Treatment with NaHS increased the activities of catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR) and ascorbate peroxidase (APX); promoted the accumulation of total phenols (TP) and ascorbic acid (AsA), and inhibited the increase in reactive oxygen species (ROS), malondialdehyde (MDA) and lipoxygenase (LOX) activity, which in turn alleviated oxidative damage. H₂S treatment down-regulated the expression of PsACO1 and PsACS1 and inhibited the production of ethylene; up-regulated the expression of PsSiR, PsSAT, PsOASTL, and PsLCD, which in turn elevated the activities of sulfite reductase (SiR), serine acetyltransferase (SAT), O-acetylserine cleavage lyase (OASTL), and L-cysteine desulfurylase (LCD), and facilitated the accumulation of the endogenous H₂S content; up-regulated the expression of L-Arginine synthesis-related genes and PsNOA, which increased the content of L-Arginine as well as the activity of nitric oxide synthase (NOS) and facilitated the synthesis of nitric oxide (NO). The above results indicated that NaHS could improve the antioxidant system, endogenous H₂S, ethylene, and NO metabolism of plum, thus maintaining the storage quality of the fruit.

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引用次数: 0

Revealing cold plasma-mediated changes in wolfberry wax and related gene expression during storage

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology

Pub Date : 2025-01-10 DOI: 10.1016/j.postharvbio.2025.113390

Yuhang Du , Kunfeng Liu , Shaofeng Yuan , Hang Yu , Yahui Guo , Yuliang Cheng , Weirong Yao

Cold plasma (CP) is promising for postharvest storage and processing of fruit. However, as the first surface material that CP interacts with, the effects of CP on the cuticular wax of fruit remain unclear. This study aimed to elucidate how direct treatment with dielectric barrier discharge plasma, at varying power levels, affects the structure, composition, and biosynthesis of wolfberry wax, and its implications on postharvest storage and drying. Our findings reveal a power-dependent response. High-power CP (>20 W) increased wax hydrophilicity, accelerating drying but potentially compromising storage stability due to increased water loss. Conversely, low-power CP (<20 W) caused minimal initial damage, with partial recovery observed during storage. Notably, 10 W treatment even promoted alkane accumulation after two days, potentially enhancing storage quality. High-power CP (40 W) induced changes in wax composition, transforming alkanes into alcohols, ketones, fatty acids, and lipids, while reducing long-chain alkanes and fatty acids, and increasing short-chain alcohols and esters. These changes were linked to the downregulation of genes involved in alkane hydroxylation, wax precursor synthesis, and the acyl reduction pathway. In contrast, 10 W treatment exhibited a milder and more targeted impact on specific wax biosynthesis steps. These findings provide insights into CP's effects on waxes, guiding CP processing for wolfberries and other fruit to enhance postharvest quality.

{"title":"Revealing cold plasma-mediated changes in wolfberry wax and related gene expression during storage","authors":"Yuhang Du , Kunfeng Liu , Shaofeng Yuan , Hang Yu , Yahui Guo , Yuliang Cheng , Weirong Yao","doi":"10.1016/j.postharvbio.2025.113390","DOIUrl":"10.1016/j.postharvbio.2025.113390","url":null,"abstract":"<div><div>Cold plasma (CP) is promising for postharvest storage and processing of fruit. However, as the first surface material that CP interacts with, the effects of CP on the cuticular wax of fruit remain unclear. This study aimed to elucidate how direct treatment with dielectric barrier discharge plasma, at varying power levels, affects the structure, composition, and biosynthesis of wolfberry wax, and its implications on postharvest storage and drying. Our findings reveal a power-dependent response. High-power CP (>20 W) increased wax hydrophilicity, accelerating drying but potentially compromising storage stability due to increased water loss. Conversely, low-power CP (<20 W) caused minimal initial damage, with partial recovery observed during storage. Notably, 10 W treatment even promoted alkane accumulation after two days, potentially enhancing storage quality. High-power CP (40 W) induced changes in wax composition, transforming alkanes into alcohols, ketones, fatty acids, and lipids, while reducing long-chain alkanes and fatty acids, and increasing short-chain alcohols and esters. These changes were linked to the downregulation of genes involved in alkane hydroxylation, wax precursor synthesis, and the acyl reduction pathway. In contrast, 10 W treatment exhibited a milder and more targeted impact on specific wax biosynthesis steps. These findings provide insights into CP's effects on waxes, guiding CP processing for wolfberries and other fruit to enhance postharvest quality.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"222 ","pages":"Article 113390"},"PeriodicalIF":6.4,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143101866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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