Pub Date : 2025-02-01DOI: 10.1016/j.psj.2024.104636
Przemysław Sołek , Anna Stępniowska , Oliwia Koszła , Jan Jankowski , Katarzyna Ognik
The poultry industry relies extensively on antibiotics and coccidiostats as essential tools for disease management and productivity enhancement. However, increasing concerns about antimicrobial resistance (AMR) and the toxicological safety of these substances have prompted a deeper examination of their broader impacts on animal and human health. This study investigates the toxicological effects of antibiotics and coccidiostats on the gut-brain axis and microbiota in turkeys, with a particular focus on molecular mechanisms that may influence neurochemical and inflammatory responses. Our findings reveal that enrofloxacin exposure leads to the upregulation of BDNF, suggesting a neuroprotective effect, while monensin treatment significantly increased eEF2 kinase expression, indicative enhanced neuronal activity. In turkeys infected with Avian Pathogenic Escherichia coli (APEC), early administration of doxycycline and monensin significantly upregulated the mTOR/BDNF and Akt/mTOR pathways, along with elevated histamine levels, underscoring their role in inflammatory responses modulation. However, treatments administered at 50 days post-hatch did not significantly alter protein levels, though both enrofloxacin and monensin increased serotonin and dopamine levels, suggesting potential neurotoxicological impacts on mood and cognitive functions. These results highlight the complex interactions between antibiotic use, gut microbiota alterations, and neurochemical pathways, with toxicological implications for environmental pollution and public health. This research provides critical insights into the potential toxic effects of prolonged antibiotic and coccidiostat exposure in poultry production, emphasizing the need for responsible use to mitigate risks to ecosystems and human health.
{"title":"Antibiotics/coccidiostat exposure induces gut-brain axis remodeling for Akt/mTOR activation and BDNF-mediated neuroprotection in APEC-infected turkeys","authors":"Przemysław Sołek , Anna Stępniowska , Oliwia Koszła , Jan Jankowski , Katarzyna Ognik","doi":"10.1016/j.psj.2024.104636","DOIUrl":"10.1016/j.psj.2024.104636","url":null,"abstract":"<div><div>The poultry industry relies extensively on antibiotics and coccidiostats as essential tools for disease management and productivity enhancement. However, increasing concerns about antimicrobial resistance (AMR) and the toxicological safety of these substances have prompted a deeper examination of their broader impacts on animal and human health. This study investigates the toxicological effects of antibiotics and coccidiostats on the gut-brain axis and microbiota in turkeys, with a particular focus on molecular mechanisms that may influence neurochemical and inflammatory responses. Our findings reveal that enrofloxacin exposure leads to the upregulation of BDNF, suggesting a neuroprotective effect, while monensin treatment significantly increased eEF2 kinase expression, indicative enhanced neuronal activity. In turkeys infected with Avian Pathogenic <em>Escherichia coli</em> (APEC), early administration of doxycycline and monensin significantly upregulated the mTOR/BDNF and Akt/mTOR pathways, along with elevated histamine levels, underscoring their role in inflammatory responses modulation. However, treatments administered at 50 days post-hatch did not significantly alter protein levels, though both enrofloxacin and monensin increased serotonin and dopamine levels, suggesting potential neurotoxicological impacts on mood and cognitive functions. These results highlight the complex interactions between antibiotic use, gut microbiota alterations, and neurochemical pathways, with toxicological implications for environmental pollution and public health. This research provides critical insights into the potential toxic effects of prolonged antibiotic and coccidiostat exposure in poultry production, emphasizing the need for responsible use to mitigate risks to ecosystems and human health.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 2","pages":"Article 104636"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732450/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.psj.2024.104737
Tieshan Xu , Zixin Xu , Dingping Bai , Fanghu Wu , Yuanyuan Shang , Mao Li , Guang Rong , Lihong Gu
In order to provide a low-cost, high efficient, and highly accurate tool for molecular breeding of Jiaji ducks, we constructed a cGPS(Genotyping by Pinpoint Sequencing of captured targets) 20 K liquid-phase microarray using resequencing data from this valuable poultry breed for the first time. The microarray contains 20,327 high-quality snp loci, mainly from the 30 Jiaji duck resequencing samples collected in this study, and some loci were supplemented from the 135 duck resequencing data from KUNMING INSTITUTE OF ZOOLOGY.CAS. This microarray showed excellent performance in two production tests. The microarray was used to genotype a population of 236 Jiaji ducks, and the genotyping data were then used for population structure analysis and genome-wide association studies (GWAS) of plumage color phenotypes. According to the population structure analysis, the population of Jiaji ducks could be divided into four subpopulations using genetic distance matrices. Using GWAS analysis, 38 significant SNP loci were identified within a region on chromosome 14 that contained 30 genes. Among them, EDNRB2 and VAMP7 were identified as strong candidate genes for the regulation of plumage color in Jiaji ducks. Two mutations upstream of EDNRB2 were identified as tightly linked to the colorless phenotype. In addition, two KASP markers were designed for the SNP loci associated with EDNRB2 (HIC_SCAFFOLD_14_14984620, HIC_SCAFFOLD_14_15016766). The KASP genotyping results showed strong correlations between different genotypes on the SNP locus HIC_SCAFFOLD_14_15016766 and the plumage phenotype. In conclusion, this independently designed microarray will be useful for large-scale genotyping and can lay the foundation for future screening of mutation loci and functional genes.
{"title":"Development and application of a cGPS 20K liquid-phase SNP microarray in Jiaji ducks","authors":"Tieshan Xu , Zixin Xu , Dingping Bai , Fanghu Wu , Yuanyuan Shang , Mao Li , Guang Rong , Lihong Gu","doi":"10.1016/j.psj.2024.104737","DOIUrl":"10.1016/j.psj.2024.104737","url":null,"abstract":"<div><div>In order to provide a low-cost, high efficient, and highly accurate tool for molecular breeding of Jiaji ducks, we constructed a cGPS(Genotyping by Pinpoint Sequencing of captured targets) 20 K liquid-phase microarray using resequencing data from this valuable poultry breed for the first time. The microarray contains 20,327 high-quality snp loci, mainly from the 30 Jiaji duck resequencing samples collected in this study, and some loci were supplemented from the 135 duck resequencing data from KUNMING INSTITUTE OF ZOOLOGY.CAS. This microarray showed excellent performance in two production tests. The microarray was used to genotype a population of 236 Jiaji ducks, and the genotyping data were then used for population structure analysis and genome-wide association studies (GWAS) of plumage color phenotypes. According to the population structure analysis, the population of Jiaji ducks could be divided into four subpopulations using genetic distance matrices. Using GWAS analysis, 38 significant SNP loci were identified within a region on chromosome 14 that contained 30 genes. Among them, <em>EDNRB2</em> and <em>VAMP7</em> were identified as strong candidate genes for the regulation of plumage color in Jiaji ducks. Two mutations upstream of <em>EDNRB2</em> were identified as tightly linked to the colorless phenotype. In addition, two KASP markers were designed for the SNP loci associated with <em>EDNRB2</em> (HIC_SCAFFOLD_14_14984620, HIC_SCAFFOLD_14_15016766). The KASP genotyping results showed strong correlations between different genotypes on the SNP locus HIC_SCAFFOLD_14_15016766 and the plumage phenotype. In conclusion, this independently designed microarray will be useful for large-scale genotyping and can lay the foundation for future screening of mutation loci and functional genes.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 2","pages":"Article 104737"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.psj.2024.104762
Biao Wang , Meiyuan Tian , Yashu Yang , Zhipeng Jia , Faisal R Anjum , Chunli Ma , Dexing Ma
In this study, phthalate inulin nanoparticles (PINs) were chemically modified and characterized. The internalization of PINs into the probiotic E. faecalis, which delivering Fiber2 protein of fowl adenovirus serotype 4 (FAdV-4), was investigated. The expression of the Fiber2 protein in E. faecalis was detected using western blot analysis. To protect recombinant E. faecalis from degradation of in the gastric acid environment, sodium alginate was used to encapsulate the bacteria. The survival ratio and release of E. faecalis in simulated gastrointestinal fluid was assessed. Oral administration of microencapsulated E. faecalis loaded with PINs (Micro-E/Fiber2-PINs) or inulin (Micro-E/Fiber2-inulin) was conducted, followed by an experimental challenge with FAdV-4 in chickens to evaluate immune responses and protection. The results showed the internalization of PINs into the bacteria promoted bacteria growth, and significantly improved the expression level of Fiber2. After incubation in simulated gastric fluid, the number of viable bacteria from the Micro-E/Fiber2-PINs group was significantly higher than that from the E. faecalis/Fiber2 group. The release of bacteria from the microcapsules was completed within 30 min. Animal experiments demonstrated that oral immunization with Micro-E/Fiber2-PINs significantly enhanced humoral and cellular immune responses, relieved inflammatory injury in FAdV-targeted organs, and improved survival rate of challenged chickens. This study presents promising potential for developing oral vaccines against pathogen infection.
{"title":"Effect of microencapsulated Fiber2-displaying probiotics loaded with inulin nanoparticles on immunity against fowl adenovirus serotype 4 in chickens","authors":"Biao Wang , Meiyuan Tian , Yashu Yang , Zhipeng Jia , Faisal R Anjum , Chunli Ma , Dexing Ma","doi":"10.1016/j.psj.2024.104762","DOIUrl":"10.1016/j.psj.2024.104762","url":null,"abstract":"<div><div>In this study, phthalate inulin nanoparticles (<strong>PINs</strong>) were chemically modified and characterized. The internalization of PINs into the probiotic <em>E. faecalis</em>, which delivering Fiber2 protein of fowl adenovirus serotype 4 (<strong>FAdV-4</strong>), was investigated. The expression of the Fiber2 protein in <em>E. faecalis</em> was detected using western blot analysis. To protect recombinant <em>E. faecalis</em> from degradation of in the gastric acid environment, sodium alginate was used to encapsulate the bacteria. The survival ratio and release of <em>E. faecalis</em> in simulated gastrointestinal fluid was assessed. Oral administration of microencapsulated <em>E. faecalis</em> loaded with PINs (<strong>Micro-E/Fiber2-PINs</strong>) or inulin (<strong>Micro-E/Fiber2-inulin</strong>) was conducted, followed by an experimental challenge with FAdV-4 in chickens to evaluate immune responses and protection. The results showed the internalization of PINs into the bacteria promoted bacteria growth, and significantly improved the expression level of Fiber2. After incubation in simulated gastric fluid, the number of viable bacteria from the Micro-E/Fiber2-PINs group was significantly higher than that from the <em>E. faecalis/</em>Fiber2 group. The release of bacteria from the microcapsules was completed within 30 min. Animal experiments demonstrated that oral immunization with Micro-E/Fiber2-PINs significantly enhanced humoral and cellular immune responses, relieved inflammatory injury in FAdV-targeted organs, and improved survival rate of challenged chickens. This study presents promising potential for developing oral vaccines against pathogen infection.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 2","pages":"Article 104762"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11761919/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.psj.2025.104771
Yuchen Jia , Lihua Li , Liai Gao
At present, in the context of the highly intensive development of livestock and poultry breeding, digital management is becoming increasingly important, and digital twin systems are gradually being applied. To solve the contradiction between data acquisition and sensor network congestion, a virtual acquisition method based on historical data and real-time reference of point data is proposed when constructing a digital twin system. Firstly, computational fluid dynamics (CFD) simulation was used to analyze and determine the temperature distribution and environmental characteristics inside the layer house, and the collection area was preliminarily divided according to the CFD simulation results. Then, combined with gray correlation degree and cosine similarity analysis, it can effectively identify the reference points highly correlated with the temperature of the key unmonitored area. Finally, WOA was used to optimize the BiLSTM hyperparameters and construct a WOA-BiLSTM virtual acquisition model. It is based on the XGBoost algorithm to determine the actual data collection points, predict the current value based on the actual data of the reference point and the historical data of the test point, and complete virtual collection. Through the test in a farm, the average absolute error between the data of 10 virtual collection points and the actual data was within 0.25 °C, which ensured the reliability of the data. It analyzes the data volume requirements for digital twin modeling and theoretically verifies the supporting role of virtual collection in the construction of digital twin systems.
{"title":"Research on virtual collection method of layer house temperature for the construction requirements of digital twin system","authors":"Yuchen Jia , Lihua Li , Liai Gao","doi":"10.1016/j.psj.2025.104771","DOIUrl":"10.1016/j.psj.2025.104771","url":null,"abstract":"<div><div>At present, in the context of the highly intensive development of livestock and poultry breeding, digital management is becoming increasingly important, and digital twin systems are gradually being applied. To solve the contradiction between data acquisition and sensor network congestion, a virtual acquisition method based on historical data and real-time reference of point data is proposed when constructing a digital twin system. Firstly, computational fluid dynamics (CFD) simulation was used to analyze and determine the temperature distribution and environmental characteristics inside the layer house, and the collection area was preliminarily divided according to the CFD simulation results. Then, combined with gray correlation degree and cosine similarity analysis, it can effectively identify the reference points highly correlated with the temperature of the key unmonitored area. Finally, WOA was used to optimize the BiLSTM hyperparameters and construct a WOA-BiLSTM virtual acquisition model. It is based on the XGBoost algorithm to determine the actual data collection points, predict the current value based on the actual data of the reference point and the historical data of the test point, and complete virtual collection. Through the test in a farm, the average absolute error between the data of 10 virtual collection points and the actual data was within 0.25 °C, which ensured the reliability of the data. It analyzes the data volume requirements for digital twin modeling and theoretically verifies the supporting role of virtual collection in the construction of digital twin systems.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 2","pages":"Article 104771"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762178/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.psj.2025.104863
Xiaopeng Li , Xiaoyong He , Guibi Li , Zhujun Wang , Fuli Huang , Jiasen Chen , Yang Song , Tanze Liu , Zhaoyan Chen , Xiangfeng Wang , Jiwei Hu , Hua He , Hehe Liu , Liang Li , Jiwen Wang , Shenqiang Hu
Semen quality has an important impact on the reproductive performance of ganders, and the quantity and quality of spermatozoa in semen are the determinants of semen quality. In our practical work, a small number of azoospermic ganders were observed in adult goose breeding populations, but the underlying regulatory mechanisms remain unknown. In the present study, we firstly compared the morphological and histological differences in the testes of ganders from normozoospermic group (NG) and azoospermic group (AG), and then analyzed the testicular expression patterns of circRNAs, miRNAs, and mRNAs between the two groups by using whole-transcriptome sequencing technology. Results from histomorphological analysis demonstrated that the body weight alone was not accountable for the occurrence of gander azoospermia, and the possible cause might be the observed testicular abnormalities. At the morphological level, the left, right, and bilateral testicular weights, the right and bilateral testicular organ indexes, and the long, short, and dorsoventral diameters of the left, right and bilateral testes were significantly lower in AG than in NG (P < 0.05). At the histological level, most testicular histological parameters, such as the testicular parenchymal area, the diameter of seminiferous tubules, and the number of germ cells, were significantly higher (P < 0.05) in NG than in AG. The RNA-seq results showed that a total of 683 differentially expressed circRNAs (DEcircRNAs), 24 differentially expressed miRNAs (DEmiRNAs), and 1,118 differentially expressed Genes (DEGs) were identified in the gander testes between NG and AG. Subsequent functional enrichment analysis revealed that most of the DEGs and the target genes of DEcircRNAs and DEmiRNAs were significantly enriched in either the biological processes related to male gonad development, spermatid development, and regulation of cell differentiation or the KEGG terms including the MAPK, TGF-beta, Wnt, and cell cycle signaling pathways. By constructing the core ceRNA regulatory networks, several key DEcircRNAs, including 1:98100313|98104995, 1:171413706|171419341, 6:3414226|3418193, and 2:115876735|115880760, were identified to regulate the expression of TGFB2 and BCL2 through interactions with specific miRNAs such as novel-miR-265 and novel-miR-266, and such interactions could play crucial roles in regulating the gander testicular cell apoptosis, proliferation, and spermatogenesis. This study provides novel insights into the function and molecular mechanisms of ceRNAs in regulating the gander testicular development and semen quality.
{"title":"Identification of the crucial circ-mi-mRNA interaction networks regulating testicular development and spermatogenesis in ganders","authors":"Xiaopeng Li , Xiaoyong He , Guibi Li , Zhujun Wang , Fuli Huang , Jiasen Chen , Yang Song , Tanze Liu , Zhaoyan Chen , Xiangfeng Wang , Jiwei Hu , Hua He , Hehe Liu , Liang Li , Jiwen Wang , Shenqiang Hu","doi":"10.1016/j.psj.2025.104863","DOIUrl":"10.1016/j.psj.2025.104863","url":null,"abstract":"<div><div>Semen quality has an important impact on the reproductive performance of ganders, and the quantity and quality of spermatozoa in semen are the determinants of semen quality. In our practical work, a small number of azoospermic ganders were observed in adult goose breeding populations, but the underlying regulatory mechanisms remain unknown. In the present study, we firstly compared the morphological and histological differences in the testes of ganders from normozoospermic group (NG) and azoospermic group (AG), and then analyzed the testicular expression patterns of circRNAs, miRNAs, and mRNAs between the two groups by using whole-transcriptome sequencing technology. Results from histomorphological analysis demonstrated that the body weight alone was not accountable for the occurrence of gander azoospermia, and the possible cause might be the observed testicular abnormalities. At the morphological level, the left, right, and bilateral testicular weights, the right and bilateral testicular organ indexes, and the long, short, and dorsoventral diameters of the left, right and bilateral testes were significantly lower in AG than in NG (<em>P</em> < 0.05). At the histological level, most testicular histological parameters, such as the testicular parenchymal area, the diameter of seminiferous tubules, and the number of germ cells, were significantly higher (<em>P</em> < 0.05) in NG than in AG. The RNA-seq results showed that a total of 683 differentially expressed circRNAs (DEcircRNAs), 24 differentially expressed miRNAs (DEmiRNAs), and 1,118 differentially expressed Genes (DEGs) were identified in the gander testes between NG and AG. Subsequent functional enrichment analysis revealed that most of the DEGs and the target genes of DEcircRNAs and DEmiRNAs were significantly enriched in either the biological processes related to male gonad development, spermatid development, and regulation of cell differentiation or the KEGG terms including the MAPK, TGF-beta, Wnt, and cell cycle signaling pathways. By constructing the core ceRNA regulatory networks, several key DEcircRNAs, including <em>1:98100313|98104995, 1:171413706|171419341, 6:3414226|3418193</em>, and <em>2:115876735|115880760</em>, were identified to regulate the expression of <em>TGFB2</em> and <em>BCL2</em> through interactions with specific miRNAs such as <em>novel-miR-265</em> and <em>novel-miR-266</em>, and such interactions could play crucial roles in regulating the gander testicular cell apoptosis, proliferation, and spermatogenesis. This study provides novel insights into the function and molecular mechanisms of ceRNAs in regulating the gander testicular development and semen quality.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 3","pages":"Article 104863"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143190235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.psj.2024.104751
Xuehui Zhang , Lihua Tang , Linqing Duan , Ruihua Yang , Kangchengyin Liu , Jing Zhao , Ye Zhao , Guozhong Zhang
The aim of this study was to investigate the molecular characteristics and pathogenicity of recently isolated ILTV strains from China, thereby augmenting our understanding of its prevalence. The complete genome sequences of seven ILTV strains obtained from China between 2015 and 2019 were determined by high-throughput sequencing. Phylogenetic analysis showed that six isolates (SD2015, GD2017, SYB2018, HB201812, HB201806, and TJ2019) were classified together with CEO vaccine strains, while only one isolates LN2018 belonged to the wild-type cluster. Recombination analysis revealed compelling evidence of probable recombination events existing in SD2015, HB201806, and LN2018. Compared to TJ2019 and LN2018, chickens infected with HB201806 exhibited more severe conjunctivitis symptoms and tracheal damage, as well as higher viral load and viral shedding in the trachea. These findings will provide valuable references for further understanding the epidemiological status of ILTV and developing effective control measures.
{"title":"Molecular characteristics and pathogenicity analysis of infectious laryngotracheitis virus isolated in China from 2015 to 2019","authors":"Xuehui Zhang , Lihua Tang , Linqing Duan , Ruihua Yang , Kangchengyin Liu , Jing Zhao , Ye Zhao , Guozhong Zhang","doi":"10.1016/j.psj.2024.104751","DOIUrl":"10.1016/j.psj.2024.104751","url":null,"abstract":"<div><div>The aim of this study was to investigate the molecular characteristics and pathogenicity of recently isolated ILTV strains from China, thereby augmenting our understanding of its prevalence. The complete genome sequences of seven ILTV strains obtained from China between 2015 and 2019 were determined by high-throughput sequencing. Phylogenetic analysis showed that six isolates (SD2015, GD2017, SYB2018, HB201812, HB201806, and TJ2019) were classified together with CEO vaccine strains, while only one isolates LN2018 belonged to the wild-type cluster. Recombination analysis revealed compelling evidence of probable recombination events existing in SD2015, HB201806, and LN2018. Compared to TJ2019 and LN2018, chickens infected with HB201806 exhibited more severe conjunctivitis symptoms and tracheal damage, as well as higher viral load and viral shedding in the trachea. These findings will provide valuable references for further understanding the epidemiological status of ILTV and developing effective control measures.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 2","pages":"Article 104751"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11758210/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.psj.2024.104745
Yujia Yang , Zhiyi Yang , Xinkui Zhang , Beibei Niu , Qiuhong Huang , Yan Li , Huifang Yin , Xianpeng Zhang , Ming Liao , Weixin Jia
Avian Influenza Virus (AIV) has been prevalent worldwide in recent years, resulting in substantial economic losses in the poultry industry. More importantly, AIV is capable of cross-species transmission among mammals, posing a dormant yet considerable threat to human health and safety. In this study, two rapid detection methods for AIV based on the CRISPR-Cas13a were developed. These methods can identify AIV through the M gene and differentiate the H5, H7, and H9 subtypes via the HA gene. The first method utilizes RT-RAA isothermal amplification of the target sequence in combination with the “collateral effect” of the Cas13a protein. The results are measured using a real-time quantitative PCR instrument, with a Limit of Detection (LOD) as low as 1 copy/μL. The second method combines RT-RAA with Cas13a and a lateral flow assay, allowing results to be visually observed with the naked eye, with a LOD of 10 copies/μL. Both methods demonstrated specificity and sensitivity comparable to or exceeding that of qRT-PCR, suggesting strong potential for clinical application.
{"title":"Rapid detection of Pan-Avian Influenza Virus and H5, H7, H9 subtypes of Avian Influenza Virus using CRISPR/Cas13a and lateral flow assay","authors":"Yujia Yang , Zhiyi Yang , Xinkui Zhang , Beibei Niu , Qiuhong Huang , Yan Li , Huifang Yin , Xianpeng Zhang , Ming Liao , Weixin Jia","doi":"10.1016/j.psj.2024.104745","DOIUrl":"10.1016/j.psj.2024.104745","url":null,"abstract":"<div><div>Avian Influenza Virus <strong>(AIV)</strong> has been prevalent worldwide in recent years, resulting in substantial economic losses in the poultry industry. More importantly, AIV is capable of cross-species transmission among mammals, posing a dormant yet considerable threat to human health and safety. In this study, two rapid detection methods for AIV based on the CRISPR-Cas13a were developed. These methods can identify AIV through the M gene and differentiate the H5, H7, and H9 subtypes via the HA gene. The first method utilizes RT-RAA isothermal amplification of the target sequence in combination with the “collateral effect” of the Cas13a protein. The results are measured using a real-time quantitative PCR instrument, with a Limit of Detection <strong>(LOD)</strong> as low as 1 copy/μL. The second method combines RT-RAA with Cas13a and a lateral flow assay, allowing results to be visually observed with the naked eye, with a LOD of 10 copies/μL. Both methods demonstrated specificity and sensitivity comparable to or exceeding that of qRT-PCR, suggesting strong potential for clinical application.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 2","pages":"Article 104745"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750554/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.psj.2024.104743
Shuo Wang , Chunbo Wei , Jiaxin Yan , Ying Zhang
This study aimed to investigate the effects of dietary metabolizable energy levels on various performance indicators of medium-sized geese, with the goal of predicting the optimal range of these energy levels. A comprehensive literature search was conducted across several databases, including Chinese National Knowledge Infrastructure, Wanfang, China Science and Technology Journal Database, PubMed, Web of Science, and ScienceDirect, covering the period from January 1, 2000, to July 1, 2024. The gathered studies focused on the impact of dietary metabolizable energy levels on the production performance of medium-sized geese. Following the literature review, a network meta-analysis was conducted by Stata software (StataCorp version 14.0). The analysis included tests for inconsistencies in the results by inconsistency model, assessment of publication bias by funnel plots, and data sorting through the robust error meta-regression model. Additionally, nonlinear dose-response curves were plotted by the restricted cubic spline method to explore the relationships between metabolizable energy levels and production performance indicators. Out of 2267 identified studies, 25 met the inclusion criteria and were incorporated into the systematic review, representing a total sample of 3538 geese. The production performance indicators examined included average daily gain, average daily feed intake, feed-to-gain ratio, and abdominal fat percentage. The results of the network meta-analysis revealed that both direct and indirect comparisons could be made across different dietary metabolizable energy levels reported in the studies. It was observed that increasing dietary metabolizable energy levels significantly impacted certain performance indicators of medium-sized geese. The findings were robust and reliable for further analysis. The dose-response meta-analysis indicated optimal dietary metabolizable energy levels within the existing ranges, specifically 13 to 13.5 MJ/kg for medium-sized geese during the brooding stage, and 12 to 13 MJ/kg during the growing stage. Future research should explore the effects of dietary metabolizable energy levels in large-sized geese, small-sized geese, and diets with higher energy levels to broaden the understanding of energy optimization across different goose sizes and developmental stages.
本试验旨在研究饲粮代谢能水平对中型鹅各生产性能指标的影响，以预测各代谢能水平的最佳范围。在中国国家知识基础设施、万方、中国科技期刊库、PubMed、Web of Science和ScienceDirect等数据库中进行了全面的文献检索，检索时间为2000年1月1日至2024年7月1日。所收集的研究主要集中在饲粮代谢能水平对中型鹅生产性能的影响。在文献综述之后，采用Stata软件（StataCorp version 14.0）进行网络meta分析。分析包括通过不一致模型检验结果的不一致性，通过漏斗图评估发表偏倚，并通过稳健误差元回归模型对数据进行排序。利用有限三次样条法绘制非线性剂量-响应曲线，探讨代谢能水平与生产性能指标之间的关系。在确定的2267项研究中，有25项符合纳入标准，纳入系统评价，代表3538只鹅的总样本。试验的生产性能指标包括平均日增重、平均日采食量、料重比和腹脂率。网络荟萃分析的结果显示，可以对研究中报告的不同饮食代谢能水平进行直接和间接比较。由此可见，饲粮代谢能水平的提高显著影响了中等鹅的某些生产性能指标。这些发现对于进一步的分析是可靠的。剂量-效应荟萃分析表明，饲粮代谢能水平在现有范围内为适宜水平，育雏期为13 ~ 13.5 MJ/kg，生长期为12 ~ 13 MJ/kg。未来的研究应进一步探讨饲粮代谢能水平对大鹅、小鹅和高能量饲粮的影响，以拓宽对不同体型鹅和不同发育阶段能量优化的认识。
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Pub Date : 2025-02-01DOI: 10.1016/j.psj.2024.104703
Khaled H. El-Kholy , Mohamed E. Sokariaa , Mohammed F. El Basuini , Sara F. Fouda , Tarek A. Ebeid , Ibrahim T. El-Ratel , Mahmoud M. Azzam , Alessandro Di Cerbo , Ahmed A. Elolimy , Mahmoud Madkour , Mahmoud Alagawany
To enhance the health and performance of poultry, novel approaches have to be created. Using appropriate nutritional interventions to enhance body physiology and thus enhance productivity is one of these approaches. The purpose of the present investigation intended to examine how growing quail physiology and growth is affected by supplementing diets with tryptophan (Trp) and/or canthaxanthin (CX). The sum of 200 unsexed, 1-week-age Japanese quails (Coturnix coturnix japonica), with a nearly similar body weight (BW) of 33.50 ± 1.20 g, were assigned, in random, to four experimental groups. Each group consisted of five replicates, with 10 birds per replicate. Chicks in group 1 (T1) served as the control and were fed a basal diet without any supplementation from week 1 to week 5. The second (T2) and third (T3) groups received feed supplemented with 0.01 % Trp and 0.005 % CX, respectively. The fourth group (T4) was given a diet containing a combination of 0.01 % Trp and 0.005 % CX. Results indicated that supplementation with Trp, CX, or their combination significantly (P < 0.05) enhanced live BW and body weight gain (BWG) at 5 weeks. No noticeable variations in carcass characteristics were found across all treatments over the whole trial duration. Blood levels of high-density lipoprotein were considerably greater in the Trp and/or CX-fed group than in the control group. Adding Trp and/orCX to quail diets significantly (P < 0.05) decreased the activity of liver enzymes (alanine transaminase, ALT; aspartate transaminase, AST; alkaline phosphatase, ALP), along with reduced low-density lipoprotein (LDL) levels. Birds received diets with Trp and/or CX had higher values of antioxidant indices in serum and liver (P < 0.05), accompanied by low values of malondialdehyde compared to control group. We concluded that adding quail diet with Trp and/orCX had positive consequences on the growth performance and some physiological indices.
{"title":"Tryptophan and/or canthaxanthin in quail diets: effects on performance, carcass traits, hematology, blood chemistry and hepatic antioxidant capacity","authors":"Khaled H. El-Kholy , Mohamed E. Sokariaa , Mohammed F. El Basuini , Sara F. Fouda , Tarek A. Ebeid , Ibrahim T. El-Ratel , Mahmoud M. Azzam , Alessandro Di Cerbo , Ahmed A. Elolimy , Mahmoud Madkour , Mahmoud Alagawany","doi":"10.1016/j.psj.2024.104703","DOIUrl":"10.1016/j.psj.2024.104703","url":null,"abstract":"<div><div>To enhance the health and performance of poultry, novel approaches have to be created. Using appropriate nutritional interventions to enhance body physiology and thus enhance productivity is one of these approaches. The purpose of the present investigation intended to examine how growing quail physiology and growth is affected by supplementing diets with tryptophan (Trp) and/or canthaxanthin (CX). The sum of 200 unsexed, 1-week-age Japanese quails (<em>Coturnix coturnix japonica</em>), with a nearly similar body weight (BW) of 33.50 ± 1.20 g, were assigned, in random, to four experimental groups. Each group consisted of five replicates, with 10 birds per replicate. Chicks in group 1 (T1) served as the control and were fed a basal diet without any supplementation from week 1 to week 5. The second (T2) and third (T3) groups received feed supplemented with 0.01 % Trp and 0.005 % CX, respectively. The fourth group (T4) was given a diet containing a combination of 0.01 % Trp and 0.005 % CX. Results indicated that supplementation with Trp, CX, or their combination significantly (<em>P</em> < 0.05) enhanced live BW and body weight gain (BWG) at 5 weeks. No noticeable variations in carcass characteristics were found across all treatments over the whole trial duration. Blood levels of high-density lipoprotein were considerably greater in the Trp and/or CX-fed group than in the control group. Adding Trp and/orCX to quail diets significantly (<em>P</em> < 0.05) decreased the activity of liver enzymes (alanine transaminase, ALT; aspartate transaminase, AST; alkaline phosphatase, ALP), along with reduced low-density lipoprotein (LDL) levels. Birds received diets with Trp and/or CX had higher values of antioxidant indices in serum and liver (<em>P</em> < 0.05), accompanied by low values of malondialdehyde compared to control group. We concluded that adding quail diet with Trp and/orCX had positive consequences on the growth performance and some physiological indices.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 2","pages":"Article 104703"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754116/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142927159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.psj.2025.104784
Guoming Li
Computer vision has progressively advanced precision poultry farming. Despite this substantial increase in research activity, computer vision in precision poultry farming still lacks large-scale, open-access datasets with consistent evaluation metrics and baselines, which makes it challenging to reproduce and validate comparisons of different approaches. Since 2019, several image/video datasets have been published and open-accessed to alleviate the issue of dataset scarcity. However, there is no a dedicated survey summarizing the existing progress. To fill this gap, the objective of this research was to provide the first survey and analysis of the open-access image/video dataset for precision poultry farming. A total of 20 qualified images/video datasets were summarized, including 4 for behavior monitoring, 6 for health status identification, 3 for live performance prediction, 4 for product quality inspection, and 3 for animal trait recognition. Critical points of creating a new image/video dataset, consisting of data acquisition, augmentation, annotation, sharing, and benchmarking, were discussed. The survey provides options for selecting appropriate datasets for model development and optimization while delivering insights into building new datasets for precision poultry farming.
{"title":"A survey of open-access datasets for computer vision in precision poultry farming","authors":"Guoming Li","doi":"10.1016/j.psj.2025.104784","DOIUrl":"10.1016/j.psj.2025.104784","url":null,"abstract":"<div><div>Computer vision has progressively advanced precision poultry farming. Despite this substantial increase in research activity, computer vision in precision poultry farming still lacks large-scale, open-access datasets with consistent evaluation metrics and baselines, which makes it challenging to reproduce and validate comparisons of different approaches. Since 2019, several image/video datasets have been published and open-accessed to alleviate the issue of dataset scarcity. However, there is no a dedicated survey summarizing the existing progress. To fill this gap, the objective of this research was to provide the first survey and analysis of the open-access image/video dataset for precision poultry farming. A total of 20 qualified images/video datasets were summarized, including 4 for behavior monitoring, 6 for health status identification, 3 for live performance prediction, 4 for product quality inspection, and 3 for animal trait recognition. Critical points of creating a new image/video dataset, consisting of data acquisition, augmentation, annotation, sharing, and benchmarking, were discussed. The survey provides options for selecting appropriate datasets for model development and optimization while delivering insights into building new datasets for precision poultry farming.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 2","pages":"Article 104784"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762189/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142966509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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