Poultry Science最新文献

{"title":"Salvage pathway of vitamin B12 absorption in chickens with mutant tumor virus a receptor","authors":"Yun Ji Shin ,&nbsp;Jin-Kyoo Kim ,&nbsp;Seung Je Woo ,&nbsp;Byung Chul Park ,&nbsp;Jae Yong Han","doi":"10.1016/j.psj.2024.104744","DOIUrl":"10.1016/j.psj.2024.104744","url":null,"abstract":"<div><div>The tumor virus A receptor (TVA), a member of the low-density lipoprotein receptor (LDLR) family, serves as an entry receptor for Avian Leukosis Virus (ALV) subgroups A and K, as well as a receptor for vitamin B₁₂ bound to transcobalamin. Naturally occurring genetic variants in the <em>TVA</em> gene determine susceptibility or resistance to ALV-A and -K, but the effects of these mutated <em>TVA</em> on vitamin B₁₂ uptake have not been investigated systemically. We found four <em>TVA</em> variants comprising the wild type (<em>TVA</em>^WT), a single nucleotide polymorphism variant (<em>TVA</em>^SNP), and two partial deletions in the splicing branch point region (<em>TVA</em>^R). This study investigates the relationship between the various genotypes of <em>TVA</em> alleles and uptake of vitamin B₁₂ in chickens. A protein interaction model suggested that mutant <em>TVA</em>s (i.e., <em>TVA</em>^SNP, <em>TVA</em>^R) may have reduced ability to take up vitamin B₁₂ due to a disrupted LDL-A domain, a pivotal region involved in vitamin B₁₂ uptake; however, we found no significant difference in absorption of vitamin B₁₂ in <em>TVA</em>^WT and <em>TVA</em>^SNP chickens, or levels of its metabolite in serum. Notably, <em>TVA</em>^R chickens had significantly higher levels of vitamin B₁₂ than <em>TVA</em>^WT chickens, a finding contrary to the predicted lower uptake. Expression of vitamin B₁₂ carrier related genes (i.e., <em>AMN, GIF,</em> and <em>TCN2</em>) in chickens showed a stepwise increase: <em>TVA</em>^WT &gt; <em>TVA</em>^SNP &gt; <em>TVA</em>^R. These results suggest a mechanism by which mutant <em>TVA</em> chickens with a disrupted TVA protein acquire natural resistance to ALV-A -K, with no impairment of vitamin B₁₂ metabolism.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 2","pages":"Article 104744"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11758413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142927067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement of the accuracy of breeding value prediction for egg production traits in Muscovy duck using low-coverage whole-genome sequence data","authors":"Haoqiang Ye ,&nbsp;Congliang Ji ,&nbsp;Xiaoqi Liu ,&nbsp;Semiu Folaniyi Bello ,&nbsp;Lijin Guo ,&nbsp;Xiang Fang ,&nbsp;Duo Lin ,&nbsp;Yu Mo ,&nbsp;ZhiLin Lei ,&nbsp;Bolin Cai ,&nbsp;Qinghua Nie","doi":"10.1016/j.psj.2025.104812","DOIUrl":"10.1016/j.psj.2025.104812","url":null,"abstract":"<div><div>Low-coverage whole genome sequencing (lcWGS) is an effective low-cost genotyping technology when combined with genotype imputation approaches. It facilitates cost-effective genomic selection (GS) programs in agricultural animal populations. GS based on lcWGS data has been successfully applied to livestock such as pigs and donkeys. However, its effectiveness in poultry is poorly reported. Furthermore, due to the high linkage disequilibrium (LD) between markers and the high marker density in lcWGS data, it is necessary to explore how to effectively utilize lcWGS data for genomic prediction. Phenotypic data for egg production traits were collected from a population of 1491 Muscovy ducks, with 975 of them sequenced using low-coverage whole genomic sequencing at an average depth of ∼0.84x. In the prediction, we compared the pedigree-based best linear unbiased prediction (PBLUP) method, the genomic best linear unbiased prediction (GBLUP) method utilizing SNP marker data, and the single-step genomic best linear unbiased prediction (SSGBLUP) method, which integrates both pedigree and SNP marker information. Among the SNP-based approaches, we further extended our analysis by applying LD-based weighting of SNPs and employing a Gaussian kernel model to capture epistatic genetic effects. The result showed that the estimated heritability of egg production traits in Muscovy duck ranged from 0.071 to 0.573. Compared to the PBLUP, integrating lcWGS data and pedigree data through a single-step genetic evaluation improved the accuracy of genomic prediction for all traits in this study, with accuracy improvement ranging from 12.3 % to 43.9 % in random cross-validation. Additionally, compared to the GBLUP, the extended method of GBLUP that controls for LD heterogeneity and accounts for epistatic effects using lcWGS data showed a superior prediction performance, with accuracy improvement ranging from 0.6 %∼75.1 % in the optimal scenario. This study demonstrates that utilization of lcWGS data is a promising approach for genomic prediction of egg production traits in Muscovy duck. Our findings provide valuable strategies for optimizing genomic prediction methods using lcWGS data.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 2","pages":"Article 104812"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11786738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging and characterization of a novel fowl adenovirus 4 strain with open reading frame 19 (ORF19) in diseased chickens from China","authors":"Jing-Ying Liao ,&nbsp;Yin Shi ,&nbsp;Hui Tang,&nbsp;Ping Fu,&nbsp;Jie-Yu Li,&nbsp;Yi-Shuai Zhang,&nbsp;Qian Wu,&nbsp;You-Song Peng,&nbsp;Chao-Ting Xiao","doi":"10.1016/j.psj.2024.104702","DOIUrl":"10.1016/j.psj.2024.104702","url":null,"abstract":"<div><div>The highly virulent genotype fowl adenovirus 4 (FAdV-4), associated with severe hydropericardium hepatitis syndrome (HHS) in poultry from China, is characterized by a large deletion of 1966 bp (1966-del), including losses of ORF19 and ORF27. However, how this virus originated remains unclear. In this study, a novel FAdV-4 strain, HNU-XXY-2019, from diseased chickens, which has a genome size of 45669 bp and is approximately 1966 bp longer than the known FAdV-4 genome from China, was isolated and characterized. Forty-eight unique amino acid substitutions were revealed in protein-coding regions, including 10 substitutions in the Fiber 2 protein, which is reported to reside in the virulence gene. Phylogenetic analyses revealed that HNU-XXY-2019 was located in an evolutionary branch between clusters of highly pathogenic FAdV-4 1966-del strains and traditional FAdV-4 strains, with a closer relationship to Indian and Parkistan strains, suggesting that it may be an intermediate strain or an ancestral strain of the FAdV-4 1966-del strains. Further animal experiments demonstrated that HNU-XXY-2019 has moderate virulence and is pathogenic to SPF chickens at high challenge doses. This study is the first discovery and characterization of a novel FAdV-4 strain with a genome similar to that of traditional FAdV-4 strains but with a close genetic relationship to the FAdV-4 1966-del strains in China, indicating that it may play an important role as an intermediate evolutionary link between the highly virulent FAdV-4 1966-del strains prevalent in China and the traditional strains circulating outside. These data also help to understand the confounding circulation of FAdV-4 and to develop new prevention methods.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 2","pages":"Article 104702"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential gene expression in Clostridium perfringens during pre-and post-infection phases and in jejunal tissues of broilers with necrotic enteritis induced by Clostridium perfringens alone and its coinfection with Eimeria","authors":"Chiao-Hsu Ke ,&nbsp;Cheng-En Wu ,&nbsp;Fan Lin ,&nbsp;Wen-Yuan Yang","doi":"10.1016/j.psj.2024.104682","DOIUrl":"10.1016/j.psj.2024.104682","url":null,"abstract":"<div><div>The pathogenesis of necrotic enteritis (<strong>NE</strong>) involves complex gene regulation at both the bacterial cell and host tissue levels, yet many aspects remain incompletely understood. This study aims to compare the differential transcriptome of the <em>netB</em>-positive <em>Clostridium perfringens</em> strain, CP54, before and after infection. Differentially expressed genes and pathways were also examined in jejunal tissues from CP54-induced and CP54-<em>Eimeria</em> coinfected NE models to identify potential targets for mitigating NE. Forty-one-day-old Cobb straight-run broilers were assigned to four groups: CP and Eimeria coinfection group (<strong>EimCP</strong>), <em>Eimeria</em> infection group (<strong>Eim</strong>), CP54 infection group (<strong>CP</strong>), and untreated control (<strong>CTL</strong>). Subclinical and severe NE models were established by oral infection with CP54 alone and CP54-<em>Eimeria</em> coinfection, respectively. Three post-infection CP54 strains (CPd1, CPd2, and CPd3) were isolated from necrotic jejunal mucosa in the EimCP group and analyzed alongside pre-infection CP54 using 4-plex bacterial RNA sequencing (<strong>RNA-seq</strong>). Jejunal tissues were collected and analyzed for differentially expressed genes between groups via tissue RNA-seq. The results showed that post-infection CP54 strains exhibited unique gene regulation patterns associated with environmental adaptation, including upregulation of oxidation-reduction processes, oxidoreductase activity, and downregulation of pyrimidine metabolism. However, no differential expressed virulence genes, including cpa<em>, colA, lepB, luxS, nanI,</em> netB, and <em>cpb2</em>, were identified between the pre- and post-infection CP54 cells. In host tissues, the analysis revealed significant activation of cytokine-cytokine receptor interactions and Toll-like receptor pathways that contribute to inflammatory responses. Upregulating IL8, IL12B, and INHBA played a key role in activating these pathways. Additionally, NE-infected jejunal tissues displayed suppressed PPAR pathway activity and increased p53 signaling. These changes suggest a significant role for apoptosis, immune regulation, and lipid metabolism in the progression of the disease. In summary, this study identifies key genes and transcripts associated with NE at both the bacterial and host levels, offering perspectives on the pathways driving disease progression and host-pathogen interactions. These findings provide crucial insights for developing effective prevention and control strategies, ultimately reducing NE risks and associated losses in the broiler industry.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 2","pages":"Article 104682"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11730944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamics of immune responses following duck Tembusu virus infection in adult laying ducks reveal the effect of age-related immune variation on disease severity","authors":"Teerawut Nedumpun ,&nbsp;Kanana Rungprasert ,&nbsp;Patchareeporn Ninvilai ,&nbsp;Benchaphorn Limcharoen ,&nbsp;Wikanda Tunterak ,&nbsp;Duangduean Prakairungnamthip ,&nbsp;Navapon Techakriengkrai ,&nbsp;Wijit Banlunara ,&nbsp;Sanipa Suradhat ,&nbsp;Aunyaratana Thontiravong","doi":"10.1016/j.psj.2024.104731","DOIUrl":"10.1016/j.psj.2024.104731","url":null,"abstract":"<div><div>Duck Tembusu virus (DTMUV), an emerging avian pathogenic flavivirus, is notably associated with neurological disorders and acute egg drop syndrome in ducks. We previously demonstrated that the susceptibility of ducks to DTMUV infection varies significantly with age, with younger ducks (4-week-old) exhibiting more severe disease than older ducks (27-week-old). However, the immunological mechanisms underlying these age-related differences in disease severity remain unclear. In this study, we investigated the dynamics of immune responses following DTMUV infection in adult laying ducks (27-week-old) and compared them to our previous findings on young ducks (4 weeks old). The numbers of T helper, cytotoxic T, B, and non-T and B lymphocytes, as well as neutralizing antibody levels, were measured in parallel with DTMUV loads in the blood and target organs. Our results revealed that the number of non-T and B lymphocytes/myeloid cells in 27-week-old adult laying ducks infected with DTMUV remained consistently stable throughout the observation period, in contrast to findings in 4-week-old younger ducks, where myeloid cell responses were implicated in disease progression. Regarding lymphocyte responses, unlike in 4-week-old younger ducks, only cytotoxic T lymphocyte responses in 27-week-old older ducks showed a significant negative correlation with the reduction of viremia and viral loads in target organs, indicating their role in controlling viral replication in older ducks. Additionally, 27-week-old adult laying ducks infected with DTMUV exhibited high levels of neutralizing antibodies, which were significantly correlated with reduced viral loads in blood and target organs. Overall, the presence of robust DTMUV-specific neutralizing antibody and CTL responses, along with a finely tuned myeloid cell response likely plays a significant role in controlling severe neurological outcomes in 27-week-old adult laying ducks. This study highlights the age-related differences in immune responses following DTMUV infection, which potentially contribute to the varying disease severity among ducks of different ages. Understanding the interplay between the host and DTMUV provides significant implications for disease management strategies and vaccine development.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 2","pages":"Article 104731"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750524/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of two in vitro methods progressed in a computer-controlled simulated digestion system to determine amino acid digestibility of feed ingredients for yellow-feathered roosters","authors":"Yao Yu ,&nbsp;Yuming Wang ,&nbsp;Kaijing Ge ,&nbsp;Jiang Chen ,&nbsp;Jingjing Xie ,&nbsp;Yi Zou ,&nbsp;Songbai Liu ,&nbsp;Huize Tan ,&nbsp;Feng Zhao","doi":"10.1016/j.psj.2024.104738","DOIUrl":"10.1016/j.psj.2024.104738","url":null,"abstract":"<div><div>This experiment compared amino acid (<strong>AA</strong>) digestibility assessed by 2 in vitro methods using a computer-controlled simulated digestion system and in vivo assay for corn, soybean meal, casein, corn gluten meal, cottonseed meal, rapeseed meal and a corn-soybean meal diet. In vitro method 1 simulated gizzard digestion at pH 2.0, followed by small intestinal digestion, and the subsequent clearance of the digested product from dialysis tubing. In vitro method 2 was similar to the first method, except that pH in gizzard digestion was 3.5 and there was an enzymatic inactivation stage before digested product clearance. Each in vitro method included 5 replicates per treatment, with 1 digestion tube per replicate. Cecectomized Chinese yellow-feathered roosters (average body weight of 2.73 kg) were assigned to 1 of the following treatments: corn, soybean meal, or a corn-soybean meal diet (<em>n</em> = 6 replicates of 3 roosters per treatment); or casein, corn gluten meal, cottonseed meal, or rapeseed meal (<em>n</em> = 5 replicates of 3 roosters per treatment) in a completely randomized design to assess digestibility of AA. The relative deviation was within 5% comparing methods 1, 2 and in vivo method for 87.5% and 92.0% of all AA digestibility measured of 7 samples, respectively. Significant linear relationships were observed between in vitro methods 1 and 2 for the digestibility of 14 AAs (except for Cys) and total amino acid (<strong>TAA</strong>) (<em>r</em> ≥ 0.778; <em>P</em> &lt; 0.05). Significant linear relationships were found between in vitro method 1 and in vivo results for 9 AAs (except for His, Ile, Asp, Cys, Glu and Ser) and TAA (<em>r</em> ≥ 0.866; <em>P</em> &lt; 0.05). Similarly, significant linear relationships between in vitro method 2 and in vivo findings were observed for 11 AAs (except for His, Lys, Cys and Glu) and TAA (<em>r</em> ≥ 0.776; <em>P</em> &lt; 0.05). The linear regression of in vivo assay on in vitro method 1 or 2 overlapped with <em>Y</em> = <em>X</em> for 7 AA and TAA or 10 AA and TAA, respectively. Our findings suggest in vitro method 2 is superior to method 1 for estimating AA digestibility of yellow-feathered roosters, this indicates that gizzard pH or inactivation of enzymes modulates the effectiveness of in vitro digestibility assays.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 2","pages":"Article 104738"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11758556/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142927897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined transcriptome and metabolome analysis of stable knockdown and overexpression of the CD8A gene in chicken T lymphocytes","authors":"Yanli Du ,&nbsp;Kun Wang ,&nbsp;Xiannian Zi ,&nbsp;Xiao Wang ,&nbsp;Meiquan Li ,&nbsp;Bo Zhang ,&nbsp;Jinshan Ran ,&nbsp;Wei Huang ,&nbsp;Jing Wang ,&nbsp;Cuilian Dong ,&nbsp;Hanyi Xiang ,&nbsp;Li Lei ,&nbsp;Changrong Ge ,&nbsp;Yong Liu","doi":"10.1016/j.psj.2024.104686","DOIUrl":"10.1016/j.psj.2024.104686","url":null,"abstract":"<div><div>CD8 subunit alpha (<strong>CD8A</strong>) is an important gene in immunity and is involved in the functional regulation of T lymphocytes. However, the specific role and regulatory mechanism of CD8A in chicken T lymphocytes remain unknown. In this study, we overexpressed and interfered with CD8A in chicken T lymphocytes and found that interfering with CD8A expression inhibited the proliferation and induced the apoptosis of T lymphocytes and that the overexpression of CD8A promoted T lymphocyte activation. Additionally, transcriptomic and metabolomic analyses of chicken T lymphocytes with CD8A overexpression or interference were performed. The overexpression and interference of the CD8A gene caused widespread changes in gene and metabolite expression in chicken T cells. The results of the transcriptome analysis revealed that differentially expressed genes (<strong>DEGs</strong>) caused by altered expression of the CD8A gene were associated with multiple “neuroactive ligand-receptor interaction”, “cell adhesion molecules”, “calcium signaling pathway”, etc. The metabolome analysis results revealed that different metabolites (<strong>DMs</strong>) caused by altered CD8A gene expression were associated with “Glutathione metabolism”, “Arginine biosynthesis”, “D-amino acid metabolism”, etc. The combined transcriptional and metabolic analysis revealed one metabolically related pathway, “Glutathione metabolism”. Our findings further revealed that interference and overexpression of CD8A plays a role in the metabolism of Glutathione. Thus, CD8A may be a critical regulator of “Glutathione metabolism” and may subsequently affect T-cell function in chickens. These results provide an important reference for further research on the effect of CD8A on the immune performance of chickens.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 2","pages":"Article 104686"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11748709/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The characterization of outer membrane vesicles (OMVs) and their role in mediating antibiotic-resistance gene transfer through natural transformation in Riemerella anatipestifer","authors":"Mengying Wang ,&nbsp;Yizhou Yao ,&nbsp;Yuhao Yang ,&nbsp;Dekang Zhu ,&nbsp;Mingshu Wang ,&nbsp;Renyong Jia ,&nbsp;Shun Chen ,&nbsp;Xinxin Zhao ,&nbsp;Qiao Yang ,&nbsp;Ying Wu ,&nbsp;Shaqiu Zhang ,&nbsp;Juan Huang ,&nbsp;Xumin Ou ,&nbsp;Bin Tian ,&nbsp;Di Sun ,&nbsp;Ling Zhang ,&nbsp;Yanling Yu ,&nbsp;Yu He ,&nbsp;Zhen Wu ,&nbsp;Anchun Cheng ,&nbsp;Mafeng Liu","doi":"10.1016/j.psj.2024.104730","DOIUrl":"10.1016/j.psj.2024.104730","url":null,"abstract":"<div><div><em>Riemerella anatipestifer</em> (<em>R. anatipestifer</em>, RA) is the etiological agent of duck serositis, an acute multisystemic disease in ducks that is globally distributed and causes serious economic losses in the duck industry. Despite exhibiting multidrug resistance, the transmission mechanism of its antibiotic resistance genes (ARGs) remains incompletely identified. To contribute to addressing this gap, in this study, outer membrane vesicles (OMVs) from the RA strain CH-1 were isolated and characterized to investigate their involvement in ARG transfer in RA. Sequencing and data analysis revealed that RA CH-1 OMVs had ∼2.04 Mb genomic size, representing 88.3 % of the RA CH-1 genomic length. Proteomic analysis showed that OMVs contained 577 proteins, representing 27.2 % of the bacterial proteins. Subsequent investigations demonstrated that OMVs from antibiotic-resistant strains transferred ARG fragments and plasmids to the sensitive strain RA ATCC11845, relying on the natural transformation system, and the transformants exhibited corresponding resistance. Overall, OMV-mediated horizontal transfer of ARGs serving as a significant mechanism for acquiring multiple resistance genes in <em>R. anatipestifer</em>.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 2","pages":"Article 104730"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of quantitative trait loci and candidate genes associated with growth curve parameters in chinese wenshang barred chickens","authors":"Yan Zhou ,&nbsp;Jie Liu ,&nbsp;Qiuxia Lei ,&nbsp;Haixia Han ,&nbsp;Wei Liu ,&nbsp;Dapeng Li ,&nbsp;Yan Sun ,&nbsp;Dan Hao ,&nbsp;Fuwei Li ,&nbsp;Dingguo Cao ,&nbsp;Jie Wang","doi":"10.1016/j.psj.2025.104767","DOIUrl":"10.1016/j.psj.2025.104767","url":null,"abstract":"<div><div>The growth curve is a vital instrument for assessing and forecasting weight and developmental shifts in livestock and poultry, which reflects the changes of bodyweight traits with time and plays a key role in guiding breeding and production approaches. This study performed a genome-wide association study (GWAS) for growth curve parameters generated by nonlinear models which fit original weight-age records, to discover the SNPs and candidate genes correlated with growth traits. Data from 362 Chinese Wenshang Barred Chickens weighed at the age of 0, 2, 4, 6, 8, 10, 12, 14, 16, and 18 weeks were used to fit the Gompertz, Logistic, and von Bertalanffy growth models. The Gompertz model showed the highest coefficient of determination (R² = 0.974). The mature body weight (<strong>A</strong>), time scale (<strong>b</strong>), and maturity rate (<strong>k</strong>) were treated as phenotypes for single-trait GWAS. The GWAS identified 44, 103, and 5 significant single nucleotide polymorphisms (<strong>SNPs</strong>) associated with A, b, and K, respectively. Among them, several candidate genes, including <em>LDB2, TOB2, RCBTB1, KPNA3, SLIT2, LCORL, LAP3</em>, and <em>TPRA1</em>, were previously reported to be associated with growth and development. Two lead SNPs (4:76022389, 4:76070237) on the <em>LDB2</em> gene were significantly associated with the growth curve. Further research of these candidate genes could help explore the full genetic architecture underlying growth and development traits in poultry.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 2","pages":"Article 104767"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11761894/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"JTF-SqueezeNet: A SqueezeNet network based on joint time-frequency data representation for egg-laying detection in individually caged ducks","authors":"Siting Lv ,&nbsp;Yuanyang Mao ,&nbsp;Youfu Liu ,&nbsp;Yigui Huang ,&nbsp;Dakang Guo ,&nbsp;Lei Cheng ,&nbsp;Zhuoheng Tang ,&nbsp;Shaohai Peng ,&nbsp;Deqin Xiao","doi":"10.1016/j.psj.2025.104782","DOIUrl":"10.1016/j.psj.2025.104782","url":null,"abstract":"<div><div>Accurate individual egg-laying detection is crucial for eliminating low-yielding breeder ducks and improving production efficiency. However, existing methods are often expensive and require strict environmental conditions. This study proposes a data processing method based on wearable sensors and joint time-frequency representation (TFR), aimed at accurately identifying egg-laying in ducks. First, the sensors continuously monitor the ducks' activity and collect corresponding X-axis acceleration data. Next, a sliding window combined with Short-Time Fourier Transform (STFT) is applied to convert the continuous data into spectrograms within consecutive windows. SqueezeNet is then used to detect spectrograms containing key features of the egg-laying process, marking these as egg-laying state windows. Finally, Kalman filtering was used to continuously predict the detected egg-laying status, allowing for the precise determination of the egg-laying period. The best detection performance was achieved by applying the 10-fold cross-validation to a dataset of 59,135 spectrograms, using a window size of 50 min and a step size of 3 min. This configuration yielded an accuracy of 95.73 % for detecting egg-laying status, with an inference time of only 2.1511 milliseconds per window. The accuracy for identifying the egg-laying period reached 92.19 %, with a precision of 93.57 % and a recall rate of 91.95 %. Additionally, we explored the scalability of the joint time-frequency representation to reduce the computational complexity of the model.</div></div>","PeriodicalId":20459,"journal":{"name":"Poultry Science","volume":"104 2","pages":"Article 104782"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11782797/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}