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Unveiling the role of long non-coding RNAs in chicken immune response to highly pathogenic avian influenza H5N1 infection. 揭示长非编码 RNA 在鸡对高致病性禽流感 H5N1 感染的免疫反应中的作用。
IF 3.8 1区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-11-06 DOI: 10.1016/j.psj.2024.104524
Thi Hao Vu, Chaeeun Kim, Anh Duc Truong, Jun-Mo Kim, Hyun S Lillehoj, Yeong Ho Hong

Avian influenza viruses (AIVs) pose a significant threat to global poultry production, necessitating effective control strategies to mitigate economic losses and ensure animal welfare. Long non-coding RNAs (lncRNAs) have emerged as crucial regulators of immune responses, yet their roles in AIV-infected chickens remain poorly understood. This study aimed to investigate the expression profiles of lncRNAs and their targets in Vietnamese Ri chickens infected with the highly pathogenic AIV (HPAIV) H5N1. Through RNA sequencing, we identified novel lncRNAs and analyzed differentially expressed (DE) transcripts at 1 and 3 days post-infection (dpi) in chicken lung tissue. Our results revealed a higher number of DE lncRNAs and mRNAs at 1 dpi and 3 dpi, respectively, compared to control, with resistant chickens exhibiting a notably stronger immune response than susceptible chickens at 3 dpi. Functional analysis implicated these lncRNAs in immune-related pathways crucial for host responses to H5N1 viral infection. Furthermore, we identified lncRNA-mRNA interactions associated with antiviral responses and immune function. Notably, several genes involved in antiviral resistance and immune responses showed higher expression in resistant chickens, confirming their stronger antiviral response. Overall, our study provides insights into the role of lncRNAs in the host's response to HPAIV H5N1 infection in chickens and highlights potential candidates for further investigation into host-pathogen interactions. These findings could drive the development of novel control strategies for AIVs, significantly enhancing poultry health and biosecurity.

禽流感病毒(AIVs)对全球家禽生产构成了重大威胁,因此必须采取有效的控制策略来减少经济损失并确保动物福利。长非编码 RNA(lncRNA)已成为免疫反应的关键调节因子,但它们在 AIV 感染鸡中的作用仍鲜为人知。本研究旨在调查lncRNA及其靶标在感染高致病性AIV(HPAIV)H5N1的越南里鸡中的表达谱。通过RNA测序,我们鉴定了新型lncRNAs,并分析了感染后1天和3天(dpi)鸡肺组织中的差异表达(DE)转录本。我们的结果显示,与对照组相比,1 dpi 和 3 dpi 的 DE lncRNA 和 mRNA 数量更多,3 dpi 时,耐药鸡的免疫反应明显强于易感鸡。功能分析表明,这些lncRNA参与了对宿主应对H5N1病毒感染至关重要的免疫相关通路。此外,我们还发现了与抗病毒反应和免疫功能相关的lncRNA-mRNA相互作用。值得注意的是,一些参与抗病毒和免疫反应的基因在耐药鸡中的表达量更高,这证实了它们更强的抗病毒反应能力。总之,我们的研究深入揭示了lncRNA在宿主对鸡感染高致病性禽流感病毒(HPAIV)H5N1的反应中的作用,并突出了进一步研究宿主-病原体相互作用的潜在候选基因。这些发现可能会推动新型禽流感病毒控制策略的开发,从而大大提高家禽健康和生物安全水平。
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引用次数: 0
Low protein diet influences mineral absorption and utilization in medium-growing yellow-feathered broilers from 1 to 30 days of age. 低蛋白日粮影响 1 至 30 日龄中生长黄羽肉鸡对矿物质的吸收和利用。
IF 3.8 1区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-11-05 DOI: 10.1016/j.psj.2024.104512
Xiaoran Li, Chong Wang, Shunying Li, Liyang Zhang, Xiudong Liao, Lin Lu

Reduced-protein diet can save protein ingredients and reduce nitrogen (N) losses. However, the effect of low protein diet on the mineral uptake and utilization in broilers needs to be explored. The aim of this study was to investigate the effect of low-protein diet on the growth performance, N deposition, mineral contents in serum, tissues and excreta, and the activities and gene expression of related enzymes in tissues of medium-growing yellow-feathered broilers, so as to elucidate the relationship between dietary protein level and the absorption and utilization of minerals in broilers. A total of 72 1-d-old Spotted-Brown male broilers were randomly allotted to 1 of 2 treatments with 6 replicate cages of 6 birds per cage for each treatment. The dietary crude protein (CP) levels for the two treatments were 21 % (the control treatment) and 19 % (low protein treatment), respectively. The experimental period was 30 d. The results showed that no differences (P > 0.05) were detected in average daily gain, average daily feed intake and feed: gain ratio of broilers during 1 to 30 d between the two treatments. However, low protein intake increased (P < 0.05) N retention rate, serum P, Cu and Mn, and excreta Cu, Mn and Zn, and decreased (P < 0.05) liver P and excreta P. In addition, birds fed low protein diet had higher (P < 0.05) manganese superoxide dismutase, and total superoxide dismutase activities in liver, and total antioxidant capacity and malondialdehyde content in heart, and lower (P < 0.05) copper-zinc superoxide dismutase (CuZnSOD) and succinate dehydrogenase activities in liver and CuZnSOD mRNA level in heart. In conclusion, the reduction of dietary CP content from 21 % to 19 % improved N retention, the absorption of P, Cu and Mn, as well as the antioxidant ability of liver and heart, and influenced metabolic utilization of P, Cu, Zn, Fe and Mn in medium-growing yellow-feathered broilers from 1 to 30 d of age.

低蛋白日粮可以节省蛋白质原料,减少氮(N)的损失。然而,低蛋白日粮对肉鸡矿物质吸收和利用的影响仍有待探讨。本研究旨在探讨低蛋白日粮对中生长黄羽肉鸡生长性能、氮沉积、血清、组织和排泄物中矿物质含量以及组织中相关酶的活性和基因表达的影响,从而阐明日粮蛋白质水平与肉鸡矿物质吸收和利用的关系。将 72 只 1 日龄斑褐雄性肉鸡随机分配到 2 个处理中的 1 个处理,每个处理 6 个重复笼,每笼 6 只。两种处理的日粮粗蛋白(CP)水平分别为 21%(对照处理)和 19%(低蛋白处理)。实验结果表明,两种处理的肉鸡在 1 至 30 天内的平均日增重、平均日采食量和料重比均无差异(P > 0.05)。然而,低蛋白日粮增加了(P < 0.05)氮的保留率、血清中的铅、铜和锰以及排泄物中的铜、锰和锌,降低了(P < 0.05)肝脏中的磷和排泄物中的磷。05),而肝脏中铜锌超氧化物歧化酶(CuZnSOD)和琥珀酸脱氢酶活性以及心脏中铜锌超氧化物歧化酶 mRNA 水平较低(P < 0.05)。总之,日粮中的 CP 含量从 21% 降至 19%,可提高 1 至 30 日龄中生长黄羽肉鸡的氮保留率、磷、铜和锰的吸收率以及肝脏和心脏的抗氧化能力,并影响磷、铜、锌、铁和锰的代谢利用。
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引用次数: 0
Research note: Simultaneous detection of GPV, H5 AIV, and GoAstV via TaqMan probe-based multiplex qPCR 研究说明:通过基于 TaqMan 探针的多重 qPCR 同时检测 GPV、H5 AIV 和 GoAstV。
IF 3.8 1区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-11-05 DOI: 10.1016/j.psj.2024.104511
Xiaoquan Wang , Miao Cai , Xiaolong Lu , Qianqian Xu , Yanhong Wang , Wenhao Yang , Kaituo Liu , Ruyi Gao , Yu Chen , Jiao Hu , Min Gu , Shunlin Hu , Xiufan Liu , Xiaowen Liu
The endemic status of goose parvovirus (GPV), H5 subtype avian influenza virus (AIV), and goose astrovirus (GoAstV) infections continues to devastate the poultry industry in China. Despite this, there exists a notable gap in the application of molecular diagnostic techniques. This investigation described the development of a multiplex qualitative polymerase chain reaction (qPCR) assay capable of concurrently detecting GPV, H5 AIV, and GoAstV, with no cross-reactivity observed with other avian viral pathogens. The assay exhibited a detection threshold of 10 copies/μL for both GPV and GoAstV, and 1 copy/μL for H5 AIV. The intra- and inter-assay coefficients of variation were < 3.0%, signifying high repeatability within and across assay batches. Utilizing this multiplex qPCR assay, a batch of 60 clinical samples was analyzed to assess its practical utility. The detected prevalence rates for GoAstV, GPV, and H5 AIV were 35.0% (21/60), 21.7% (13/60), and 15.0% (9/60), respectively. Concurrent infections were also identified, with rates for GPV + GoAstV, GPV + H5 AIV, GoAstV + H5 AIV, and GPV + GoAstV + H5 AIV being 6.7% (4/60), 3.3% (2/60), 3.3% (2/60), and 3.3% (2/60), respectively. The developed multiplex qPCR assay exhibited a diagnostic concordance rate equivalent to that of traditional PCR techniques. This novel assay serves as a rapid, efficient, specific, and sensitive tool for the detection of prevalent goose viruses, thereby enhancing disease management strategies and epidemiological monitoring efforts.
鹅细小病毒(GPV)、H5 亚型禽流感病毒(AIV)和鹅星状病毒(GoAstV)感染的地方性流行状况继续对中国的家禽业造成严重破坏。尽管如此,分子诊断技术的应用仍存在明显差距。这项调查描述了一种多重定性聚合酶链反应(qPCR)检测方法的开发过程,该方法能够同时检测 GPV、H5 AIV 和 GoAstV,而且与其他禽类病毒病原体没有交叉反应。该检测方法对 GPV 和 GoAstV 的检测阈值为 10 拷贝/μL,对 H5 AIV 的检测阈值为 1 拷贝/μL。测定内和测定间的变异系数均小于 3.0%,表明测定批次内和测定批次间的重复性很高。利用这种多重 qPCR 检测方法,对一批 60 份临床样本进行了分析,以评估其实用性。检测到的 GoAstV、GPV 和 H5 AIV 感染率分别为 35.0%(21/60)、21.7%(13/60)和 15.0%(9/60)。还发现了并发感染,GPV + GoAstV、GPV + H5 AIV、GoAstV + H5 AIV 和 GPV + GoAstV + H5 AIV 的感染率分别为 6.7%(4/60)、3.3%(2/60)、3.3%(2/60)和 3.3%(2/60)。所开发的多重 qPCR 检测法的诊断吻合率与传统 PCR 技术相当。这种新型检测方法是一种快速、高效、特异和灵敏的工具,可用于检测流行的鹅病毒,从而加强疾病管理策略和流行病学监测工作。
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引用次数: 0
A PLIN1 polymorphism is associated with fat production in male emus PLIN1多态性与雄性鸸鹋的脂肪产量有关。
IF 3.8 1区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-11-05 DOI: 10.1016/j.psj.2024.104513
Yuichi Koshiishi , Ryo Takahashi , Michiko Murata-Okubo , Yuichi Kameyama , Kousaku Souma , Hiroki Hirayama , Kenta Wada
The emu (Dromaius novaehollandiae) is a novel poultry species that produces meat, eggs, and fat. Although emus have recently been domesticated, genetic improvements to establish strains have scarcely progressed. In this study, we investigated the relationship between production traits and perilipin 1-encoding gene (PLIN1) polymorphisms in the emus. We determined the partial complementary DNA (cDNA) sequence of the PLIN1, which is involved in lipid droplet formation. We identified four nucleotide substitution sites (c.270C>T, c.321T>C, c.587A>T, and c.639C>T) in the PLIN1 gene of emus. Of these, c.587A>T is a non-synonymous substitution that converts lysine to methionine at the 196th codon (p.K196M). Although p.K196M was predicted to affect the production traits of emus, a large deflection in genotype frequency was observed in this study; thus, we could not investigate the relationship between genotypes and phenotypes. In males, the fat yields of the CC, CT, and TT genotypes in c.270C>T were 0.25 ± 0.06, 0.22 ± 0.06, and 0.21 ± 0.07 kg, respectively, while the meat yields of the CC, CT, and TT genotypes in c.270C>T were 0.15 ± 0.01, 0.16 ± 0.02, and 0.16 ± 0.03 kg, respectively. These results indicate that male emus with the CC genotype had a significantly higher fat content and lower meat productivity than male emus with the other genotypes (P < 0.05). Therefore, c.270C>T in PLIN1 affects fat and meat production in males. Our findings may contribute to the effective genetic improvement of the emus.
鸸鹋(Dromaius novaehollandiae)是一种新型家禽,可产肉、蛋和脂肪。虽然鸸鹋最近已被驯化,但建立品系的遗传改良工作进展甚微。本研究调查了鸸鹋的生产性状与围脂素 1 编码基因(PLIN1)多态性之间的关系。我们测定了参与脂滴形成的 PLIN1 的部分互补 DNA(cDNA)序列。我们在鸸鹋的 PLIN1 基因中发现了四个核苷酸替换位点(c.270C>T、c.321T>C、c.587A>T 和 c.639C>T)。其中,c.587A>T 是一个非同义替换,可将第 196 个密码子上的赖氨酸转换为蛋氨酸(p.K196M)。虽然p.K196M预计会影响鸸鹋的生产性状,但在本研究中观察到基因型频率有很大偏差,因此我们无法研究基因型与表型之间的关系。在雄性鸸鹋中,c.270C>T的CC、CT和TT基因型的脂肪产量分别为0.25 ± 0.06、0.22 ± 0.06和0.21 ± 0.07千克,而c.270C>T的CC、CT和TT基因型的肉产量分别为0.15 ± 0.01、0.16 ± 0.02和0.16 ± 0.03千克。这些结果表明,与其他基因型的雄性鸸鹋相比,CC基因型的雄性鸸鹋脂肪含量明显较高,肉产量较低(P < 0.05)。因此,PLIN1中的c.270C>T会影响雄性鸸鹋的脂肪和肉产量。我们的研究结果可能有助于鸸鹋的有效遗传改良。
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引用次数: 0
Melatonin nuclear receptors mediate monochromatic light-induced T-lymphocyte proliferation of thymus through the AKT/GSK3β/β-catenin pathway in chick 褪黑激素核受体通过AKT/GSK3β/β-catenin途径介导单色光诱导的小鸡胸腺T淋巴细胞增殖
IF 3.8 1区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-11-05 DOI: 10.1016/j.psj.2024.104507
Juanjuan Xiong , Zixu Wang , Yulan Dong , Jing Cao , Yaoxing Chen
Based on previous research, it's unclear about the signaling pathway involved in the negative regulation of T-lymphocyte proliferation in thymus by monochromatic red light. Newly hatched chicks were randomly assigned divided into white (WL), red (RL), green (GL), and blue (BL) light treatments. Three days later, each light treatment group was further divided into intact, sham operation, and pinealectomy groups. The findings revealed that RL led to an increase in the expression of RORα and RORγ, while p-AKT/p-GSK3β/β-catenin/CyclinD1 expression in the thymus of chicks were decreased. Conversely, GL showed opposite results compared to RL. After pinealectomy, accompanied with the expression of RORα and RORγ increased under four light, p-AKT/ p-GSK3β/ β-catenin/ CyclinD1 expression were decreased. In vitro, exogenous melatonin increased the p-AKT/β-catenin/CyclinD1 expression in the thymic lymphocytes of chick reared under RL. The stimulative effect of melatonin was enhanced by SR3335 (RORα antagonist) or GSK298 (RORγ antagonist), while it was attenuated by SR1078 (RORα/RORγ agonist), LY-294 (PI3K antagonist) and HY-102 (AKT antagonist). These results demonstrate that RORα/RORγ negatively regulate monochromatic red light induced-T-lymphocyte proliferation in the thymus, possibly through the PI3K/AKT/p-GSK3β (Ser9) signaling pathway.
根据以往的研究,单色红光对胸腺T淋巴细胞增殖负调控的信号通路尚不清楚。将刚孵出的小鸡随机分为白光(WL)、红光(RL)、绿光(GL)和蓝光(BL)处理组。三天后,每个光照处理组又被分为完整组、假手术组和松果体切除组。研究结果显示,RL导致小鸡胸腺中RORα和RORγ的表达增加,而p-AKT/p-GSK3β/β-catenin/CyclinD1的表达减少。相反,GL的结果与RL相反。松果体切除后,伴随着四光下RORα和RORγ表达的增加,p-AKT/ p-GSK3β/ β-catenin/CyclinD1的表达减少。在体外,外源性褪黑激素增加了RL饲养小鸡胸腺淋巴细胞中p-AKT/β-catenin/CyclinD1的表达。SR3335(RORα拮抗剂)或GSK298(RORγ拮抗剂)增强了褪黑激素的刺激作用,而SR1078(RORα/RORγ激动剂)、LY-294(PI3K拮抗剂)和HY-102(AKT拮抗剂)则减弱了这种作用。这些结果表明,RORα/RORγ可能通过PI3K/AKT/p-GSK3β(Ser9)信号通路负向调节单色红光诱导的胸腺T淋巴细胞增殖。
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引用次数: 0
Research Note: Proteases effect on soybean meal: enzyme product and area of production differences on standardized ileal amino acids digestibility of broiler chickens. 研究说明:蛋白酶对豆粕的影响:酶产品和产地对肉鸡标准化回肠氨基酸消化率的影响。
IF 3.8 1区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-11-04 DOI: 10.1016/j.psj.2024.104493
Hong Liu, Murtala Umar Faruk, Levy Teixeira, André Fávero, Sergio L Vieira

This study evaluated the effects of two protease products added to feeds having two soybean meal (SBM) sources (South and North of Brazil). A total of five hundred and sixty, 21 d male broiler chickens were allocated into 7 dietary treatments: a nitrogen-free diet (NFD) and other six diets prepared with a 40% replacement of the NFD with each SBM source. Two commercial protease products (RONOZYME® ProAct and ProAct 360TM) were added at 15,000 PROT and 30,000 NFP per kg feed, respectively. After 5-day diet adaptation, all birds were euthanized for ileal digesta collection. Results show that the North SBM sample was inherently higher (P < 0.05) in apparent ileal digestibility (AID) of dry matter (DM), nitrogen (N), and the standardized ileal amino acid digestibility (SIAAD) compared to South SBM sample. The DM digestibility and SIAAD of North SBM sample were not different from the un-treated control and protease treatments (P > 0.05, except Val and Ile, which had higher digestibility with ProAct 360). The DM digestibility and SIAAD from South SBM sample were increased (P < 0.05, except Met, Asp and Cys) with both supplemental proteases. Correlation analysis showed that AA digestibility of the two SBM samples were negatively associated with the effect of protease products. These data demonstrated that the SIAAD of SBM was influenced by SBM source with the South SBM sample being lower than that from the North while the protease effects were more evident in the SBM sample from South; responses of ProAct 360 were superior to ProAct. Exogenous proteases can enhance nutritional value of SBM, particularly for those with inherently low amino acid digestibility.

本研究评估了在两种豆粕(SBM)来源(巴西南部和北部)的饲料中添加两种蛋白酶产品的效果。总共有五百六十只 21 日龄的雄性肉鸡被分配到 7 种日粮处理中:一种无氮日粮 (NFD),以及用每种 SBM 来源替代 40% NFD 的其他六种日粮。每公斤饲料中分别添加 15,000 PROT 和 30,000 NFP 的两种商用蛋白酶产品(RONOZYME® ProAct 和 ProAct 360TM)。经过 5 天的饮食适应期后,所有禽类均被安乐死,以收集回肠消化液。结果表明,与南方 SBM 样品相比,北方 SBM 样品的干物质(DM)、氮(N)的表观回肠消化率(AID)和标准化回肠氨基酸消化率(SIAAD)都要高(P < 0.05)。北区 SBM 样品的 DM 消化率和 SIAAD 与未处理对照组和蛋白酶处理组相比没有差异(P > 0.05,但 Val 和 Ile 除外,ProAct 360 的消化率更高)。两种补充蛋白酶都提高了南方 SBM 样品的 DM 消化率和 SIAAD(P < 0.05,Met、Asp 和 Cys 除外)。相关分析表明,两种 SBM 样品的 AA 消化率与蛋白酶产品的影响呈负相关。这些数据表明,SBM 的 SIAAD 受 SBM 来源的影响,南方 SBM 样品的 SIAAD 低于北方 SBM 样品,而南方 SBM 样品的蛋白酶效果更明显;ProAct 360 的反应优于 ProAct。外源蛋白酶可以提高 SBM 的营养价值,特别是对那些氨基酸消化率低的 SBM。
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引用次数: 0
Effect of black soldier fly (Hermetia illucens) larvae meal and oil on the performance, biochemical profile, intestinal health and gut microbial dynamics in laying hens. 黑飞虱幼虫粉和油对蛋鸡生产性能、生化指标、肠道健康和肠道微生物动态的影响
IF 3.8 1区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-11-03 DOI: 10.1016/j.psj.2024.104460
Samiullah Khan, Xiaoli Shi, Renlian Cai, Zhao Shuai, Wei Mao, Ibrar Muhammad Khan, Ayman A Swelum, Jianjun Guo

This study investigated the effect of incorporating black soldier fly (BSF) larvae meal and oil on laying hens' performance, egg quality, serum profile, intestinal structure, and gut health. A total of 378 Lohmann laying hens (age 48 wk) were randomly assigned to 6 treatments with 3 replicates of 21 hens each. Following 7 d acclimation, the trail was conducted for 8 weeks. The dietary groups include: basal corn-soybean meal diet (S) without BSF (BSO) oil (S+BSO 0), S with BSF oil (S+BSO 100), BSF meal (9 %) without BSF oil (BSF 9+BSO 0), BSF meal (9 %) with BSF oil (BSF 9+BSO 100), BSF meal (18 %) without BSF oil (BSF 18+BSO 0), and BSF meal (18 %) with BSF oil (BSF 18+BSO 100). The results showed that the BSF 18 + BSO 100 diet significantly reduced egg weight (P < 0.001) compared to other dietary treatments. The addition of BSF meal reduced feed intake (P < 0.001) and the Haugh units (P < 0.05) in hens fed 18 % BSF meal with and without BSO. The jejunum villus area, crypt depth, and intestinal wall thickness increased with the increase in the inclusion of BSF larvae meal (P < 0.001). The ileum villus height, crypt depth and intestinal wall thickness increased (P < 0.001) at 9 % BSF meal and then decreased at 18 % BSF meal with and without BSF oil. The bacteria genera Ruminococcus, Clostridiales, Bacteroidales, Ruminococcus torques, and Intestinimonas were positively associated with the dietary treatments, while Prevotellaceae UCG-001, Clostridium, and Faecalibacterium were negatively associated with the dietary treatments. The inclusion of BSF meal and oil enriched the functional network of several pathways, including ascorbate and aldarate metabolism, D-arginine and D-ornithine metabolism, and fatty acid metabolism, highlighting the positive effects of BSF larvae meal and oil on the chicken gut microbiota. In conclusion, BSF meal at 9 % with BSF oil and BSF meal at 18 % without BSF oil can be incorporated into the diet without impairing the performance and gut health of laying hens.

本研究调查了添加黑斑潜蝇(BSF)幼虫粉和油对蛋鸡生产性能、鸡蛋质量、血清概况、肠道结构和肠道健康的影响。将 378 只 Lohmann 蛋鸡(48 周龄)随机分配到 6 种处理中,每种处理设 3 个重复,每个重复 21 只母鸡。经过 7 天的适应期后,进行为期 8 周的试验。日粮组包括不含 BSF(BSO)油的基础玉米-豆粕日粮(S)(S+BSO 0)、含 BSF 油的基础玉米-豆粕日粮(S)(S+BSO 100)、不含 BSF 油的 BSF 粉(9 %)(BSF 9+BSO 0)、含 BSF 油的 BSF 粉(9 %)(BSF 9+BSO 100)、不含 BSF 油的 BSF 粉(18 %)(BSF 18+BSO 0)和含 BSF 油的 BSF 粉(18 %)(BSF 18+BSO 100)。结果表明,与其他日粮处理相比,BSF 18 + BSO 100 日粮显著降低了蛋重(P < 0.001)。添加 BSF 粉降低了母鸡的采食量(P < 0.001),降低了母鸡的哈氏单位(P < 0.05)。空肠绒毛面积、隐窝深度和肠壁厚度随着 BSF 幼虫粉添加量的增加而增加(P < 0.001)。回肠绒毛高度、隐窝深度和肠壁厚度在添加 9% BSF 幼虫粉时增加(P < 0.001),然后在添加和不添加 BSF 油的 18% BSF 幼虫粉时减少。反刍球菌属、梭状芽孢杆菌属、类杆菌属、瘤胃反刍球菌属和肠杆菌属与日粮处理呈正相关,而前鞭毛菌科 UCG-001、梭状芽孢杆菌属和粪杆菌属与日粮处理呈负相关。添加 BSF 粉和油丰富了多个途径的功能网络,包括抗坏血酸和醛酸代谢、D-精氨酸和 D-鸟氨酸代谢以及脂肪酸代谢,凸显了 BSF 幼虫粉和油对鸡肠道微生物群的积极影响。总之,在蛋鸡日粮中添加含 9% BSF 幼虫粉和 BSF 油的 BSF 幼虫粉和含 18% BSF 幼虫粉但不含 BSF 油的 BSF 油不会影响蛋鸡的生产性能和肠道健康。
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引用次数: 0
The proteome and metabolome changes distinguish the effect of dietary energy levels on the development of ovary in chicken during sexual maturity. 蛋白质组和代谢组的变化区分了日粮能量水平对性成熟鸡卵巢发育的影响。
IF 3.8 1区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-11-03 DOI: 10.1016/j.psj.2024.104495
Lingqian Yin, Xinyu Wang, Donghao Zhang, Zhongzhen Lin, Yan Wang, Chunlin Yu, Hang Jie, Feng Xu, Chaowu Yang, Yiping Liu

To deeply understanding the impact of peripheral energy level on the development of ovaries during the sexual maturation of chicken, in this study, the ovaries and serum of sexually mature and immature chickens at the same age from different energy level groups were collected, and the proteome and metabolome were detected. The results of ovarian and serum metabolomics revealed that dietary energy levels affected the energy metabolism and fatty acid oxidation of ovary in chicken, including the up-regulated expression of dihydroacetone phosphate and α-linolenic acid in high energy level groups. The results of proteomics showed that peripheral energy levels affected the catecholamine biosynthesis and metabolism in ovary before sexual maturation. The integrating analysis revealed that increased energy flux may influence ovarian development by regulating cholesterol reserves and steroid hormone synthesis in the ovaries. In vitro, the cultivation of chicken primary granulosa cells showed that sterol carrier protein 2 played a role in fatty acid synthesis and metabolism but did not significantly affect progesterone synthesis. Overall, dietary energy levels may be involved in the development of the ovaries during sexual maturation by influencing energy metabolism, biosynthesis of unsaturated fatty acids and steroid hormone within the ovaries.

为深入了解外周能水平对鸡性成熟过程中卵巢发育的影响,本研究采集了不同能水平组同龄性成熟鸡和未成熟鸡的卵巢和血清,并对其蛋白质组和代谢组进行了检测。卵巢和血清代谢组学结果显示,日粮能量水平影响鸡卵巢的能量代谢和脂肪酸氧化,其中高能量组磷酸二氢丙酮和α-亚麻酸表达上调。蛋白质组学研究结果表明,外周能量水平影响了性成熟前卵巢中儿茶酚胺的生物合成和代谢。整合分析表明,能量通量的增加可能通过调节卵巢中胆固醇的储备和类固醇激素的合成来影响卵巢的发育。体外培养鸡原代颗粒细胞的结果表明,固醇载体蛋白2在脂肪酸合成和代谢中发挥作用,但对孕酮的合成没有显著影响。总之,膳食能量水平可能通过影响卵巢内的能量代谢、不饱和脂肪酸的生物合成和类固醇激素,参与卵巢在性成熟过程中的发育。
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引用次数: 0
Characterization of a novel IGFBP-2 transcript in the ovarian granulosa cells of chicken follicles: mRNA expression, function and effect of reproductive hormones and IGF1 鸡卵泡颗粒细胞中新型 IGFBP-2 转录本的特征:mRNA 表达、功能以及生殖激素和 IGF1 的影响
IF 3.8 1区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-11-02 DOI: 10.1016/j.psj.2024.104501
Longxiao Hu , Dandan Li , Qingqing Wei , Li Kang , Yi Sun , Yunliang Jiang
Insulin-like growth factor binding protein-2 (IGFBP-2), a binding protein of insulin-like growth factor (IGF) system, regulates the activity of IGFs and also influences cellular function with endogenous activity. In mammals, IGFBP-2 is reported to affect ovarian follicle development and steroidogenesis; however, its role in the chicken ovary is unknown. In this study, we investigated the mRNA expression and function of a novel IGFBP-2 transcript and the effect of reproductive hormones and insulin-like growth factor 1 (IGF1) on its expression in the ovarian granulosa cells of chicken follicles. The mRNA expression of IGFBP-2 was significantly increased in granulosa cells after follicle selection and was higher in hierarchical granulosa cells (Post-GCs) than in pre-hierarchical granulosa cells (Pre-GCs). IGFBP-2 promoted the proliferation and inhibited the apoptosis of both Pre-GCs and Post-GCs, enhanced the mRNA expression of genes involved in progesterone (P4) synthesis in Pre-GCs. However, in Post-GCs, IGFBP-2 inhibited the mRNA expression of these genes and suppressed P4 secretion. The mRNA expression of IGFBP-2 was inhibited by estradiol (E2) and follicle-stimulating hormone (FSH), but enhanced by P4 in Pre-GCs. In Post-GCs, FSH and IGF1 stimulated the mRNA expression of IGFBP-2 synergistically. Knockdown of IGFBP-2 attenuated the stimulatory effect of IGF1 on the mRNA expression of the side chain cleavage enzyme cytochrome P450 family 11 subfamily A member 1 (CYP11A1). These findings indicate that IGFBP-2 is regulated by FSH and IGF1, exerts different functions in Pre-GCs and Post-GCs in regulating IGF1 and plays an important role in chicken follicle development by affecting granulosa cell proliferation and P4 synthesis.
胰岛素样生长因子结合蛋白-2(IGFBP-2)是胰岛素样生长因子(IGF)系统的一种结合蛋白,它调节 IGFs 的活性,并以内源性活性影响细胞功能。据报道,IGFBP-2 在哺乳动物中影响卵泡发育和类固醇生成,但在鸡卵巢中的作用尚不清楚。本研究调查了一种新型 IGFBP-2 转录本的 mRNA 表达和功能,以及生殖激素和胰岛素样生长因子 1(IGF1)对其在鸡卵泡颗粒细胞中表达的影响。卵泡选择后颗粒细胞中IGFBP-2的mRNA表达量明显增加,分层颗粒细胞(Post-GCs)中IGFBP-2的mRNA表达量高于分层前颗粒细胞(Pre-GCs)。IGFBP-2能促进Pre-GCs和Post-GCs的增殖并抑制其凋亡,增强Pre-GCs中参与孕酮(P4)合成的基因的mRNA表达。然而,在后前叶细胞中,IGFBP-2 会抑制这些基因的 mRNA 表达并抑制 P4 的分泌。雌二醇(E2)和促卵泡激素(FSH)会抑制 IGFBP-2 的 mRNA 表达,但 P4 则会增强前 GCs 中 IGFBP-2 的 mRNA 表达。在Post-GCs中,FSH和IGF1协同刺激IGFBP-2的mRNA表达。敲除 IGFBP-2 可减轻 IGF1 对侧链裂解酶细胞色素 P450 家族 11 亚家族 A 成员 1(CYP11A1)mRNA 表达的刺激作用。这些研究结果表明,IGFBP-2受FSH和IGF1调控,在前GCs和后GCs中发挥不同的调节IGF1的功能,并通过影响颗粒细胞增殖和P4合成在鸡卵泡发育中发挥重要作用。
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引用次数: 0
Comparative transcriptomic analysis revealed potential mechanisms regulating the hypertrophy of goose pectoral muscles. 比较转录组分析揭示了调节鹅胸肌肥大的潜在机制。
IF 3.8 1区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2024-11-02 DOI: 10.1016/j.psj.2024.104498
Xinyue Hu, Yali Liu, Bincheng Tang, Jiwei Hu, Hua He, Hehe Liu, Liang Li, Shenqiang Hu, Jiwen Wang

Pectoral muscle development is an important economic trait. According to the different essence, muscle development can be divided into 2 processes: embryonic muscle fiber generation and postnatal muscle fiber hypertrophy, and postnatal muscle fiber hypertrophy has a greater impact on muscle development than the number of muscle fibers formed during the embryonic phase in poultry. However, the underlying mechanisms regulating the hypertrophy of goose pectoral muscles have not been elucidated. Therefore, the purpose of the present study was to conduct transcriptome sequencing in pectoral muscles of both Landes (LD) and Sichuan White (SW) geese at 6, 10, and 30 weeks of age to reveal the molecular mechanisms regulating pectoral muscle hypertrophy through intra-breed and inter-breed bioinformatics analyses. Phenotypically, the pectoral muscle weight/index of LD and SW geese increased from 6 to 30 weeks of age, and except for the pectoral muscle index at 10 weeks of age (P = 0.962), at the same age, the pectoral muscle weight/index of LD geese were significantly higher than that of SW geese (P < 0.05). In transcriptional regulation, intra-breed bioinformatics analysis identified 3331 genes whose expression levels were opposite to the trend of pectoral muscle hypertrophy both in LD and SW geese, and the 3331 genes were mainly enriched into abundant KEGG pathways related to lipid metabolism, proliferation/apoptosis, and immune response. Moreover, 23 genes (including SLC2A10, TNFRSF1A, PRKAA1, SLC27A4, ITGB2, THY1, RHOA, MYL10, ACTB, PRKCB, PIK3R2, RAC2, DMD, LATS2, YAP1, WWTR1, SMAD7, CTGF, FGF1, AXIN2, GLI2, ID2, and CCND2) who were enriched in 6 crosstalk pathways named viral myocarditis, insulin resistance, sphingolipid signaling pathway, hippo signaling pathway, chemokine signaling pathway, and leukocyte transendothelial migration were identified as the key candidate genes regulating the hypertrophy of goose pectoral muscles. In inter-breed bioinformatics analysis, abundant different expression genes (DEGs) related to lipid metabolism, immune response, and proliferation/apoptosis were identified between LD and SW geese too, and compared with SW geese, the expression level of MYL10 in LD geese was lower, while the expression levels of GLI2/CTGF/SMAD7 in LD geese were higher. These results suggested that the hypertrophy of goose pectoral muscles might be achieved through more lipid deposition and less leukocyte infiltration to promote the proliferation of cells within the muscles, and the low expression of MYL10 and high expressions of GLI2/CTGF/SMAD7 might the keys to induce the pectoral muscle hypertrophy of LD geese from 6 to 30 weeks of age over that of SW geese. All data the present study obtained will provide new insights into the molecular mechanisms regulating the hypertrophy of goose pectoral muscles.

胸肌发育是一项重要的经济性状。根据不同的本质,肌肉发育可分为胚胎期肌纤维生成和产后肌纤维肥大两个过程,而产后肌纤维肥大对家禽肌肉发育的影响要大于胚胎期形成的肌纤维数量。然而,调节鹅胸肌肥大的内在机制尚未阐明。因此,本研究的目的是对6周龄、10周龄和30周龄的兰德斯鹅(LD)和四川白鹅(SW)的胸肌进行转录组测序,通过种内和种间生物信息学分析揭示调控胸肌肥大的分子机制。从表型上看,6-30周龄LD鹅和SW鹅的胸肌重量/指数均有所增加,除10周龄的胸肌指数(P = 0.962)外,同龄LD鹅的胸肌重量/指数均显著高于SW鹅(P < 0.05)。在转录调控方面,种内生物信息学分析发现3331个基因的表达水平与LD鹅和SW鹅胸肌肥大的趋势相反,这3331个基因主要富集在与脂质代谢、增殖/凋亡和免疫应答相关的丰富的KEGG通路中。此外,23个基因(包括SLC2A10、TNFRSF1A、PRKAA1、SLC27A4、ITGB2、THY1、RHOA、MYL10、ACTB、PRKCB、PIK3R2、RAC2、DMD、LATS2、YAP1、WWTR1、SMAD7、CTGF、FGF1、AXIN2、GLI2、ID2 和 CCND2),它们在 6 个名为病毒性心肌炎的串联通路中富集、胰岛素抵抗、鞘脂信号通路、河马信号通路、趋化因子信号通路和白细胞跨内皮迁移这 6 个交叉通路中富集的基因被确定为调控鹅胸肌肥大的关键候选基因。在种间生物信息学分析中,也发现了大量与脂质代谢、免疫反应和增殖/凋亡相关的不同表达基因(DEGs),与西南鹅相比,MYL10在西南鹅中的表达水平较低,而GLI2/CTGF/SMAD7在西南鹅中的表达水平较高。这些结果表明,鹅胸肌的肥大可能是通过更多的脂质沉积和更少的白细胞浸润来促进肌肉内细胞的增殖,而MYL10的低表达和GLI2/CTGF/SMAD7的高表达可能是诱导6-30周龄LD鹅胸肌肥大超过SW鹅的关键。本研究获得的所有数据将为调控鹅胸肌肥大的分子机制提供新的见解。
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引用次数: 0
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Poultry Science
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